中国医药导报
      Home                                                                                    中文
中国医药导报
 
Forthcoming Articles
Current Issue
Archive
Advanced Search
Read Articles
Download Articles
Email Alert
 
Quick Search  
  Adv Search
2017 Vol. 14, No. 28
Published: 2017-10-05
 
 
4 The effects of γ-secretase inhibitor DAPT on proliferation and apoptosis of U2OS
HU Guohai DAI Guo LIU Gaiwei SONG Qi YU Ling YANG Jian GUO Weichun
Objective To observe the effect of gamma secretase inhibitor DAPT on proliferation and apoptosis of human osteosarcoma cell line U2OS, and to explore the possible mechanism of DAPT inhibiting osteosarcoma proliferation and inducing apoptosis. Methods Osteosarcoma cell line U2OS was cultured in vitro. Cell proliferation was tested by cell counting kit-8 (CCK-8) assay. Double stained by Annexin V-FITC and Propidium Iodide (PI), the cells were detected by flow cytometry (FCM) for apoptosis. Hoechst 33258 was used to detect the morphological change of typical apoptotic cells. The apoptosis related mRNA and protein levels in U2OS were detected by RT-qPCR and Western blot. The activities of Caspase-3, Caspase-8 and Caspase-9 were measured with Caspase activity assay kit. Results CCK-8 assay showed that 5-50 μmol/L DAPT inhibited the proliferation of human osteosarcoma U2OS cells and in a time- and dose-dependent manners. Flow cytometry showed that 5, 10 and 20 μmol/L DAPT could significantly induce apoptosis of osteosarcoma U2OS cells, compared to the control group, the differences were statistically significant (P < 0.05). Apoptotic body could be observed by Hoechst 33258 staining after treatment with 5, 10 and 20 μmol/L DAPT. RT-PCR and Western blot analysis showed that after treatment with DAPT (5, 10, 30 μmol/L), the expression of Caspase-3, 8 ,9 mRNA were higher than those of control group (P < 0.05), the pro-apoptotic protein Bax increased (P < 0.05), and anti-apoptosis protein Bcl-2 protein decreased (P < 0.05). The activities of Caspase-3, Caspase-8 and Caspase-9 promoted obviously after treatment with DAPT (5, 10, 20, 30 μmol/L) when compared with the control group (P < 0.05). Conclusion DAPT can significantly inhibit the proliferation and induce apoptosis of human osteosarcoma cell line U2OS. Its mechanism of action may be related to activation of Caspase dependent apoptosis pathway.
2017 Vol. 14 (28): 4-8,12 [Abstract] ( 199 ) HTML (1 KB)  PDF (850 KB)  ( 163 )
9 Effects of 3-n-butylphthalide on memory and Caspase-3 expression in the hippocampal CA1 region of vascular dementia rats 
MAO Xijing ZHU Bochi YU Tingmin YAO Gang▲
Objective To study the effects of 3-n-butylphthalide (NBP) on memory and Caspase-3 expression in the hippocampal CA1 region of vascular dementia rats, in order to explore protective effects and mechanisms of NBP on rats with vascular dementia (VD). Methods A total of 80 SPF Wistar rats were divided into vascular dementia models group (VD group), vascular dementia models + NBP injection group (NBP treatment group), sham surgery + NBP injection group (NBP control group), sham surgery group (Sham group), according to random group method. Then each group was divided into four subgroups (n = 5): 1, 2, 4, and 8 weeks after surgery. VD models were established by ligating bilateral common carotid artery. Then the rats in the NBP treatment group and NBP control group were intraperitoneally injected with NBP 5 mg/（kg·d） for 7 consecutive days. The rats in the VD group and Sham group were intraperitoneally injected with saline (0.2 mL/d) for 7 consecutive days. Memory ability of 8 weeks subgroup rats were tested by Morris water maze at 4 and 8 weeks after surgery. At 1, 2, 4, and 8 weeks after surgery, the rats in each group were decapitated. The brains were obtained, and then hippocampus were isolated. Caspase-3 expression in the hippocampal CA1 region were determined by immunohistochemistry. Results Compared with VD group, escape latency in the NBP treatment group were significantly shorter (P < 0.05). At 1, 2, 4 and 8 weeks after surgery, Caspase-3 expression in the VD group and NBP treatment group were significantly increased than those in the Sham group (P < 0.05). Compared with VD group, Caspase-3 expression in the NBP treatment group were significantly lower at 4 and 8 weeks after surgery (P < 0.05). In VD group, Caspase-3 expression at 8 weeks after surgery was significantly higher than at 1 weeks (P < 0.05). Conclusion NBP can improve the memory ability of VD rats, which may be connected with the overexpression of caspase-3 in the hippocampus CA1 region of VD rats.
2017 Vol. 14 (28): 9-12 [Abstract] ( 241 ) HTML (1 KB)  PDF (643 KB)  ( 182 )
13 Experimental research on effect of hypertrophy of primary cardiomyocytes in suckling mouse induced by salt loading and aldosterone
YOU Shuping1 JIANG Hong1 HE Lijuan2 TAO Ning2 WANG Zhong2 CHEN Qingjie3 LIU Tao2
Objective To investigate the synergistic effects of salt loading and aldosterone on hypertrophy of primary cardiomyocytes in neonatal rats. Methods Neonatal rat cardiomyocytes were culturedin the experiment, the cell was divided into normal control group, Aldosterone 10-7 mol/L (Ald) group, normal sodium concentration 140 mmol/L (NS) group, high sodium concentration 146 mmol/L (HS) group, 140 mmol/L+Ald 10-7 mol/L (NS+Ald) group, 146 mmol/L+Ald 10-7mol/L (HS+Ald) group. At first Ald was not added into (NS+Ald) group and (HS+Ald) group, after 48 h culture, Ald was added, then all treatment groups were continued to culture for 24 h. The difference of myocardial cross-sectional area was measured by immunofluorescence. The survival rate was detected by CCK-8. The apoptosis rate was detected by flow cytometry. ET-1 and eNOS mRNA expressions were assayed by qRT-PCR. Results The cross-sectional area of myocardial cells in NS+Ald group and HS+Ald group were significantly higher than Ald group, NS group, HS group. Compared with normal control group, the survival rate of cardiomyocytes decreased significantly (P < 0.01) and apoptosis rate increased significantly (P < 0.01) in NS+Ald group and HS+Ald group; except for NS group, ET-1 mRNA expression increased and eNOS mRNA expression decreased in other groups, the effect of HS+Ald group was the most significant (P < 0.01). Conclusion Salt loading and aldosterone are important participation factors which induced cardiomyocyte hypertrophy, they have synergistic effects. Among them, aldosterone-induced cardiomyocyte hypertrophy is the most significant in high salt condition.
2017 Vol. 14 (28): 13-16 [Abstract] ( 260 ) HTML (1 KB)  PDF (894 KB)  ( 110 )
17 Effects of MiR-30c-2-3p on podocyte viability and expression of Nephrin and Podocin
CHEN Qi1 MIN Jingjing2 WANG Xiaoyi1▲
Objective To investigate the effect of MiR-30c-2-3p on podocyte viability and Nephrin and Podocin protein expression. Methods The model of mice podocyte injury  was induced by puromycin aminonucleoside (PAN) in vitro, the podocytes were divided into four groups: blank group, PAN group, negative plasmids+PAN group, positive plasmids+PAN group. The MiR-30c-2-3p mimics were transiently transfected with LipofectamineTM 2000, the cell viability was detected by Cell Counting Kit-8 (CCK-8), the expression of MiR-30c-2-3p was detected by real-time quantitative PCR (qRT-PCR), the expression of Nephrin and Podocin protein was detected by Western blot. Results ①CCK-8 showed that the viability of PAN group was significantly lower than that of the blank group, the differences were statistically significant (P < 0.01). There was no significant difference between the negative plasmid+PAN group and the PAN group (P > 0.05). The viability of positive plasmid+PAN group was significantly increased than that of the PAN group and the negative plasmid+PAN group, the differences were statistically significant (P < 0.05 or P < 0.01). ②The expression of MiR-30c-2-3p in the PAN group was significantly lower than that in the blank group, the differences were statistically significant (P < 0.01). There was no significant difference between the negative plasmid group and the PAN group (P > 0.05). The content of MiR-30c-2-3p in the positive plasmid+PAN group was significantly higher than that of the PAN group and the negative plasmid+PAN group, the differences were statistically significant (P < 0.05 or P < 0.01). ③The expression of Nephrin and Podocin in the PAN group were significantly lower than those in the blank group, the differences were statistically significant (P < 0.01). There was no significant difference between the negative plasmid+PAN  group and the PAN group (P > 0.05). Compared with the PAN group and the negative plasmid+PAN group, the expression of Nephrin and Podocin in the positive plasmid+PAN group increased, the differences were statistically significant (P < 0.05). Conclusion The PAN intervention inhibits the podocyte activity, MiR-30c-2-3p content, and the Nephrin and Podocin  expression, but the exogenous expression of MiR-30c-2-3p can improve the viability of podocytes and increase the expression of Nephrin and Podocin protein.
2017 Vol. 14 (28): 17-20,30 [Abstract] ( 254 ) HTML (1 KB)  PDF (795 KB)  ( 228 )
21 Study on the antimicrobial activity in vitro of Feiyang Changweiyan Capsules
LI Yao1 YANG Qian2 ZHOU Dan2 LI Hua2 SHI Zhihui3 WANG Siwang2 XIE Yanhua2
Objective To investigate the antimicrobial activity in vitro of Feiyang Changweiyan Capsules. Methods The tube double dilution method and agar plate method were used to determine the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of Feiyang Changweiyan Capsules against Escherichia coil, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, β-hemolytic streptococcus and Candida albicans, taking Ceftriaxone as positive control drug. Results The antibacterial activity of Feiyang Changweiyan Capsules against Escherichia coil (MIC was 6.25 mg/mL), Staphylococcus aureus (MIC was 1.56 mg/mL), Enterococcus faecalis (MIC was 25.00 mg/mL), Pseudomonas aeruginosa (MIC was 6.25 mg/mL) and β-hemolytic streptococcus (MIC was 12.50 mg/mL) was stronger, which of Candida albicans (MIC was 100.00 mg/mL) was weaker. Conclusion Feiyang Changweiyan Capsules have stronger antimicrobial activity in vitro for Escherichia coil, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and β-hemolytic streptococcus.
2017 Vol. 14 (28): 21-24 [Abstract] ( 263 ) HTML (1 KB)  PDF (605 KB)  ( 141 )
25 Protective effects of different processes of Qikui Particles for model rats with diabetic nephropathy
LIU Qing1,2 ZHU Xuanxuan2 WANG Qiong2 LIU Zhihui2 YU Jiangyi2 YANG Chen2 FU Rui2 ZHANG Di2
Objective To compare the protective effects of different processes of Qikui Particles for model rats with diabetic nephropathy. Methods Fifty-four healthy SD rats (male, 8 weeks old) were selected. The model rats with diabetic nephropathy were induced by high sugar/fat diet + unilateral nephritic ligation + Streptozotocin (STZ, 40 mg/kg, ip). After successful establishment of model rats, they were divided into 5 groups: model group, Irbesartan group, process 1 (water boiled concentrated decoction) group, process 2 (water boiled and alcohol extraction concentration) group, process 3 (the mixed of above two processes) group, and the blank group was set up. Qikui Particles prepared under 3 different processes were orally administered at the same dose (18.67 g/kg ) for 5 consecutive weeks. The effects of different processes of Qikui Particles on blood sugar, urinary protein, creatinine and endogenous creatinine clearance rate in rats with diabetic nephropathy were observed, and histopathology was detected. Results Compared with blank group, the body weight in model group was decreased significantly (P ＜ 0.01), the food-intake, water consumption and urine volume were increased (P ＜ 0.05 or P ＜ 0.01), the blood sugar was improved significantly (P ＜ 0.01). After drug intervention, compared with model group, the body weight in each drug administration group was increased more or less, the food-intake, water consumption and urine volume were decreased more or less; the blood sugar in process 2 group and process 3 group was decreased significantly (P ＜ 0.05); the serum creatinine in Irbesartan group and process 3 group was decreased significantly (P ＜ 0.05 or P ＜ 0.01); the endogenous creatinine clearance rate in each drug administration group was increased significantly (P ＜ 0.05 or P ＜ 0.01); the urinary protein excretion rate in Irbesartan group and process 2, 3 group was decreased significantly (P ＜ 0.01 or P ＜ 0.05). The results of histopathology showed that the main performance of kidney in model groups was glomerular hypertrophy, tubular dilation, interstitial and renal pelvis focal infiltration of inflammatory cells; compared with model group, the scope and extent of renal injury were reduced slightly in each drug administration group, especially in Irbesartan group and process 2 group. Conclusion The protective effects of different processes of Qikui Particles for diabetic nephropathy are related to the decrease of blood sugar, creatinine, urinary protein and alleviation of renal injury, especially in process 2 group and process 3 group.
2017 Vol. 14 (28): 25-30 [Abstract] ( 276 ) HTML (1 KB)  PDF (1023 KB)  ( 189 )
31 Research on quality standard of Gouju Capsules
ZHU Heping HAN Xiaoke WANG Quan QI Jun
Objective To establish the quality standard of Gouju Capsules. Methods The main ingredients of Ramudus Uncaria Cum Uncis, Epimedium were identified by TLC. Content of icariin was determined by UV-HPLC. The content of the berberine was determined by HPLC; the column of Agilent ZORBAX SB-C18(150 mm×4.6 mm, 5 μm) was used. The mobile phase was ocetonitrile-water (25∶75), the column temperature of 30℃, at the flow of 1.0 mL/min and detection wavelength was 270 nm. Results The spots on TLC plates were distinct, without interference. A good linearity of icariin was in the ranges of 15-150 μg/mL (r=0.9996). The average recovery was 100.9% (RSD =2.77%). Conclusion The results of this experiment are accurate, simple and easy to control. It can be used as a quality standard for Gouju Capsules.
2017 Vol. 14 (28): 31-33,37 [Abstract] ( 213 ) HTML (1 KB)  PDF (571 KB)  ( 163 )
34 Studies on the extracting and antioxidant activities of total flavonoids in Mongolian medicine Gymnadenia conopsea
WU Weidong1 YANG Laixiu2 WU Junting2 SU Kemeng2 WEN Aiping2
Objective To optimize the extraction process of total flavonoids in Gymnadenia conopsea, establish a method of its content determination and research its antioxidant activities. Methods The orthogonal experiments was used to optimize the extraction process of total flavonoids in Gymnadenia conopsea. The content of total flavonoids were determined using rutin as reference by colorimetric. The antioxidant activity was evaluated by hydroxyl free radical scavenging activity. Results The optimized extraction conditions were 90% ethanol as solvent, material -liquid ratio of 1∶20, reflux extraction 2 times, each time 60 min. Rutin showed good linear relationship in the range of 0.1094-0.8752 mg, the average recovery was 98.8%, RSD was 1.05%. The total flavonoids possessed potential scavenging activity of hydroxyl free radical. Conclusion The optimum extraction process is stable, reliable, the method of content determination is simple, accurate, reproducible. The content of total flavonoids is quite different in Gymnadenia conopsea of different areas. The total flavonoids in Gymnadenia conopsea have obvious antioxidant activities.
2017 Vol. 14 (28): 34-37 [Abstract] ( 214 ) HTML (1 KB)  PDF (534 KB)  ( 128 )
38 Characteristics and clinical application of nalbuphine hydrochloride
YU Qiao1 CHEN Chun2
Nalbuphine Hydrochloride ("Nalbuphine" for short) is a synthetic opioid agonist-antagonist analgesic derivative of the phenanthree group, it can be combined with μ, κ, δ receptor, activating κ receptors, providing central analgesic and sedative effects; partially antagonized the μ opioid receptor, thus reducing the side effects associated with the excitement of opioid receptor, especially onrespiratory depression, nausea, vomiting and pruritus. Nalbuphine is mainly metabolized by liver, clearanced by intestinal, a small part clearanced by the renal. The analgesic effect of nalbuphineis rapid onset , long duration of action. On abroad, it has been used for postoperative analgesia and analgesia in gynecological and pediatric surgery; in the domestic, since the market Nalbuphine has attracted a lot of attention, mainly used for mild to moderate pain caused by various reasons, due to the low rate of adverse reactions, so it has broad application prospects.
2017 Vol. 14 (28): 38-41 [Abstract] ( 289 ) HTML (1 KB)  PDF (417 KB)  ( 330 )
42 Advances in pharmacological effects of ginsenoside Rh2
XU Wen CAO Hui YANG Yuanrong
Ginsenoside Rh2 has be extracted from red ginseng as a diol-based low-sugar saponin monomer and one of the main active ingredients in ginseng. Ginsenoside Rh2 is discovered that it has a variety of pharmacological activity by pharmacy workers in the depth of the study , and was made some progress especially in anti-tumor activity. the pharmacological effects of ginsenoside Rh2 are analyzed and summarized by The authors from a lot of literature at home and abroad on the aspects of anti-tumor, improving myocardial ischemia, anti-endometriosis, anti-allergy, immune function, radiation protection, anti-asthma and antidepressant activity and mechanism of action, this review studies and develops the use of ginsenoside Rh2 in order to provide reference.
2017 Vol. 14 (28): 42-45 [Abstract] ( 245 ) HTML (1 KB)  PDF (419 KB)  ( 292 )
46 Analysis of Treg cells and lymphocyte subgroup in 131 patients with ankylosing spondylitis
WEI Yunyu1 HAN Zhijun1▲ HUANG Hongyu1 DU Wei2 REN Tianli3 GAO Mingzhu1
Objective To analyze the changes of regulatory T cells (Treg cells) and the subsets of lymphocytes as well as the HLA-B27 expression in peripheral blood of ankylosing spondylitis (AS) patients, and then explore their roles in the development of AS. Methods Flow cytometry was applied. From February 2013 to December 2016, Department of Orthopedic and Rheumatology in Wuxi Second People's Hospital, 131 cases of AS patients were selected as AS group, 127 healthy subjects were selected as normal control group. The peripheral blood Treg cells, lymphocyte subsets and HLA-B27 were detected. The relationship of Treg cells, lymphocyte subsets, HLA-B27 and AS patients were analyzed. Results 131 cases of AS group, HLA-B27 positive for 123 cases (93.89%), the positive rate of HLA-B27 in normal control group was 2 cases (1.57%), the difference was statistically significant (P < 0.05). The positive rate of CD3+CD4+, CD4/CD8 and CD3-CD19+ in AS group were significantly higher than those in normal control group (P < 0.05). The positive rate of CD3+CD8+, CD16+/CD56+ in AS group were lower than those in normal control group (P < 0.05). The positive rate of CD4+CD25high and CD4+CD25highCD127low in AS group were lower than those in normal control group, the differences were statistically significant (P < 0.05). There was no significant difference in the positive rate of CD3+ and CD3+CD4+ between two groups (P > 0.05). Conclusion HLA-B27 has a strong correlation with AS. There are significant differences in the subgroups of lymphocytes in peripheral blood between AS and normal control group. At the same time, peripheral blood Treg cells in AS patients are significantly reduced. The above findings suggest that cellular immunity and humoral immunity may play an important role in AS.
2017 Vol. 14 (28): 46-48 [Abstract] ( 236 ) HTML (1 KB)  PDF (654 KB)  ( 121 )
49 The efficacy of the interferon-γ release assay for diagnosing cervical tuberculous lymphadenitis
XU Hui1 YAN Guangpeng1 MA Jin2 TANG Hui2 XIA Zhigang2 YU Jing3 LI Jun1
Objective To evaluate the performance of interferon-γ release assay (IGRAs) in patients with suspected cervical tuberculous lymphadenitis. Methods From December 2014 to April 2016, 94 patients with abnormal cervical lymph node in People′s Hospital of Xinjiang Uygur Autonomous Region were categorized into cervical tuberculous lymphadenitis group (52 cases) and nonactive cervical tuberculous lymphadenitis group (42 cases) according to histopathologic and microbiological findings. IGRAs using peripheral blood mononuclear cells was performed to examine the IFN-γ response to the Mycobacterium tuberculosis-specific antigens. The number of cells (SFCs) in two groups was compared. Results SFCs median in cervical tuberculous lymphadenitis group (90) was significantly higher than that in non-cervical tuberculous lymphadenitis group (0), the difference was statistically significant (P ＜ 0.05). The sensitivity of peripheral blood gamma-interferon release assay was 92.31% (48/52), the specificity was 69.05% (29/42), the positive predictive value was 78.69%, the negative predictive value was 87.88%. Conclusion In a high tuberculosis burden setting, results of IGRAs are of limited value in the diagnosis of cervical tuberculous lymphadenitis.
2017 Vol. 14 (28): 49-52 [Abstract] ( 285 ) HTML (1 KB)  PDF (638 KB)  ( 140 )
53 Partial correlation analysis of various chronic disease and osteoporosis in hospitalized patients
ZHAO Lei LIU Xueyuan CHEN Richao HE Jie LU Shuihuan LIAO Li LI Cheng
Objective To discuss the correlation between osteoporosis and various chronic diseases in the elderly. Methods 373 patients in Geriatrics Department of 181th Hospital of PLA from April 2013 to September 2016 were selected as the whole patients, in which the elderly patients over 60 years old were selected as the elderly. Within 24 hours of admission, age, sex , height and BMI, at the same time, according their anamnesis and hospital examination, whether suffered from common chronic diseases such as type-2 diabetes, hypertension, coronary heart disease, COPD, cerebral apoplexy, malnutrition, chronic kidney disease, cataract, spinal degeneration, urinary tract stones and biliary calculi of these patients were investigated. The BMD of L2-L4, bilateral collum femoris, oblique eminence of cuboid bone and Ward′s triangle were measured, the low values of T-scores were taken. Then the relation between the foregoing investigation′s data and BMD in all patients and the elderly were analyzed by partial correlation analysis. Results Partial correlation analysis showed that in all patients BMD was related with sex, age, BMI, type-2 diabetes, stroke, cataracts, thoracic vertebra degeneration, knee-joint degeneration, biliary calculi (P < 0.05). But in the elderly, the BMD was related with sex, BMI, type-2 diabetes, hypertension, coronary heart disease, cerebral apoplexy, knee-joint degeneration, biliary calculi (P < 0.05). Conclusion BMD is related with multiple factors and various chronic diseases such as gender, BMI, type-2 diabetes, stroke , knee-joint degeneration, biliary calculi and so on, meanwhile, BMD in the elderly is related to hypertension and others, which prompts that prevention and treatment of these chronic diseases may contribute to osteoporosis.
2017 Vol. 14 (28): 53-56 [Abstract] ( 235 ) HTML (1 KB)  PDF (653 KB)  ( 135 )
57 Anxiety and depression in patients with inflammatory bowel disease and its relationship with quality of life
HU Yiting1 ZHANG Jian1 JIA Guicong2 HOU Hongtao1 SU Shaohui1 BAI Yun1 WANG Yuzhen1
Objective To analyze the anxiety and depression of patients with inflammatory bowel disease, and explore their relationship with the quality of life. Methods 60 patients with inflammatory bowel disease treated in Hebei People's Hospital from September 2015 to January 2017 were selected as IBD group, and 60 cases with physical examination in the same period were selected as normal group. Self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were used to evaluate the anxiety and depression status of patients, and the quality of life was evaluated using the inflammatory bowel disease quality of life questionnaire (IBDQ). Results The incidence rates of anxiety and depression in IBD group were 45.0% and 60.0%, which were significantly higher than 3.3% and 6.7% in normal group (P < 0.05). The anxiety and depression scores in IBD group were significantly higher than those in normal group (P < 0.05). In IBD group, with different levels of anxiety and depression, the IBDQ scores of patients were also statistically significant (P < 0.05). There were significant negative correlations between SAS, SDS scores and IBDQ score in IBD patients (P < 0.05). Conclusion The incidence rates of anxiety and depression in patients with inflammatory bowel disease are significantly higher than the normal population, and the quality of life decreased with the serious anxiety and depression, with the targeted treatment can help to improve the prognosis of patients.
2017 Vol. 14 (28): 57-59,68 [Abstract] ( 274 ) HTML (1 KB)  PDF (668 KB)  ( 131 )
60 Research on intervention effect of nano silver dressing in combination with antibiotics for chronic wound infection
GUO Chunlan XI Zuyang DAI Delan
Objective To evaluate the effect of nano silver dressing in combination with antibiotics for chronic wound infection treatment, in order to provide the basis for rational use of silver dressing and optimization of wound treatment in clinic. Methods 189 cases of patients with chronic wound infection treated in Wound Outpatient of the Second Hospital of Lanzhou University, People's Hospital Affiliated to Hubei University of Medicine, the First Clinical Medical College of China Three Gorges University from January 2012 to June 2016 were selected. According to multi-center randomized controlled method, patients were divided into group A, B and C, each group had 63 cases. After the operation process unified assessment and debridement, according to the test design requirements, with the doctor's advice, group A was locally used systemic sensitive antibiotic treatment in combination with nano silver dressing process, group B was only partial used nano silver dressing treatment, group C was given intravenous infusion of sensitive antibiotics, and locally used vaseline dressing processing with 30 days intervention. All patients followed up for 30 days, they were treated by overall intervention, such as full nutrition, activity, pain, stress. The pathogens and resistant bacteria positive rates, wound scores and pH value at different time after intervention, healing time and wound healing rate within 60 days were recorded. Results With the longer duration of intervention, except the group C had no significant decreasing of positive rate of resistant bacteria, in other groups the positive rates of pathogenic bacteria and resistant bacteria, and pH values were all decreased, and wound scores increased (P < 0.05). Comparison in the three groups: after the intervention for 14 d and 21 d, the observation indexes of group A were significantly better than group B and group C, in which, group B were better than those of group C, the differences were statistically significant (P < 0.01). The wound healing rate of group A was higher than that of group B and group C, and the healing time was shorter than group B and group C, with statistically significant differences (P < 0.01). Conclusion Antibiotic treatment in combination with nano silver dressing for chronic chronic wound infection has synergistic antibacterial effect, which can optimize the wound microacid environment, promot wound repair, in order to provide the basis for the evidence-based treatment.
2017 Vol. 14 (28): 60-64 [Abstract] ( 198 ) HTML (1 KB)  PDF (674 KB)  ( 200 )
65 Clinical effect of anti-thrombosis on improved subcutaneous injection of Low Molecular Weight Heparin Calcium
JIN Dapeng1 WANG Wenfeng1 ZHAO Xiujuan2 WANG Haiyan3▲
Objective To observe clinical effect and complication of anti-thrombosis on improved subcutaneous injection of Low Molecular Weight Heparin Calcium. Methods 96 patients treated in Outpatient, Affiliated Hospital of Chengde Medical College and need to accept Low Molecular Weight Heparin Calcium antithrombotic therapy from March 2015 to February 2017 were selected as research objects. All patients were grouped according to treatment method. Low Molecular Weight Heparin Calcium modified subcutaneous injection was used in study group (48 cases), conventional injection treatment was applied in control group (48 cases). The platelet aggregation function, blood routine and coagulation function between two groups were observed before and after treatment. Complications, treatment satisfaction between two groups were compared. Results There were no significant difference in PLT, APTT, PAGm-ADP, PT levels between two groups before treatment and after treatment (P > 0.05). PAGm-ADP of two groups after treatment were lower than before treatment, the differences were statistically significant (P ＜ 0.05). The incidence rate of subcutaneous hemorrhage was 6.25% in study group, lower than that in control group (27.08%), the difference was statistically significant (P < 0.05). The incidence rate of induration was 0.00% in study group, lower than that in control group (6.25%), the difference was statistically significant (P < 0.05). Total satisfaction rate of study group was 93.75%, higher than that of control group (81.25%), the difference was statistically significant (P < 0.05). Conclusion The improved subcutaneous injection procedure applied in patients receiving Low Molecular Weight Heparin Calcium antithrombotic therapy can significantly reduce the risk of complication, ensure clinical efficacy, improve treatment satisfaction of patient.
2017 Vol. 14 (28): 65-68 [Abstract] ( 699 ) HTML (1 KB)  PDF (657 KB)  ( 81 )
69 Influence of fast track surgery on postoperative stress reaction and blood glucose level in patients with colorectal cancer and diabetes mellitus  
HE Fang WANG Pingyun LI Xinghua CHANG Weiwei
Objective To explore the influence of fast track surgery (FTS) on stress reaction and blood glucose level in the perioperative patients with colorectal cancer and diabetes mellitus. Methods A total of 124 patients with colorectal cancer and diabetes mellitus, who were admitted in the Second Hospital of Qinhuangdao from June 2013 to June 2016 were selected. All patients were divided into the control group (60 cases) and the observation group (64 cases) according to the perioperative treatment. The patients in the control group were performed with routine perioperative treatment, while the patients in the observation group were given FTS perioperative treatment. The defecation time, anal exhaust time, time of wallowing half-solid food in the two groups after operation were observed. And the stress reaction indexes (CRP, IL-6, Cor) and fasting blood glucose (FPG) level in the two groups before and after the operation were compared. Results The defecation time, anal exhaust time, time of wallowing half-solid food in the observation group were significantly shorter than those in the control group (P < 0.05). There were no significant differences in the levels of CRP, IL-6, Cor and FPG before operation in the two groups (P > 0.05), but after operation there were statistically significant differences between the two groups (P < 0.01); except the FPG in observation group on postoperative 3 day (P > 0.05), compared with before surgery of the above indicators, there were also significant differences in each group on postoperative 1 and 3 day(P < 0.01). Conclusion FTS concept used for the perioperative management of colorectal carcinoma complicated with diabetes mellitus, can reduce the stress response and the level of blood glucose, and accelerate the patients′ postoperative rehabilitation.
2017 Vol. 14 (28): 69-73 [Abstract] ( 221 ) HTML (1 KB)  PDF (664 KB)  ( 108 )
74 Clinical study of early goal-directed sedation in mechanical ventilation patients with acute exacerbation of chronic obstructive pulmonary disease
WANG Hongzhou DENG Lijuan▲ TANG Qin CHENG Senzhong
Objective To study the clinical application of early goal-directed sedation in mechanical ventilation patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Methods 90 patients with AECOPD given mechanical ventilation from July 2015 to May 2017 in Intensive Care Unit of Sichuan Mianyang 404th Hospital were randomly divided into control group (standard sedation regimen) and observation group (Dexmedetomidine early goal-directed sedation), 45 cases in each group. Sedation target was RASS -2 to 1 points. The first dose of load and maintenance doses were carried out according to the instructions. The reaching ideal sedation time, wake-up time, ICU hospitalization time, duration of mechanical ventilation, adverse reactions and levels of inflammatory cytokine were compared between the two groups. Results There was no significant difference between the two groups in reaching ideal sedation time (P > 0.05), but the wake-up time of the observation group was significantly shorter than the control group (P < 0.05). There was no significant difference between the two groups in ICU hospitalization time (P > 0.05), but the duration of mechanical ventilation of the observation group was significantly shorter than the control group (P < 0.05). The adverse reactions of the observation group were lower than those of the control group (P < 0.05). There was no statistically significant difference in plasma TNF-α level between the two groups when they were stabilized by 24 h (P > 0.05), however, the level of plasma IL-1 in the observation group was lower than that of the control group at the stabilization of 24 h (P < 0.05). Conclusion It is feasible to perform early goal-directed sedation in mechanical ventilation AECOPD patients with satisfactory sedative effect, which can shorten the duration of ICU hospitalization and reduce the adverse reactions.
2017 Vol. 14 (28): 74-77 [Abstract] ( 241 ) HTML (1 KB)  PDF (455 KB)  ( 76 )
78 Effects of different doses of Dexmedetomidine on stress response and cytokines level in patients underwent gynecological laparoscopic surgery
SHI Jianwen
Objective To explore the effect of different doses of Dexmedetomidine in gynecological laparoscopic operation and the influence on stress response and cytokines. Methods 80 cases of patients underwent gynecological laparoscopic operation in Women and Children's Hospital of Qingdao City from February 2014 to February 2016 were selected. According to the doses of Dexmetrine before anesthesia, patients were divided into two groups, each groups had 40 cases. Patients in the control group were treated with a moderate doses of Sufentanil, Rocuonium Bromide and Propofol. 0.2 μg/kg Dexmedetomidine was given 10 min before anesthesia induction, maintained by 0.5 μg/(kg·h) until 30 min before the end of the surgery. Patients in the study group were given 0.5 μg/kg Dexmedetomidine at 10 min before anesthesia induction, maintained by 1 μg/(kg·h) until 30 min before the end of the surgery. The HR, MAP and levels of TNF-α, IL-6 and IL-10 were detected at 10 min before anesthesia induction (T1), 1 min after tracheal intubation (T2), 1 min after pneumoperitoneum (T3) and the end of the operation (T4). Results At T2-T4, the MAP levels of the study group were lower than those of the control group, at T2-T3 the HR levels of the study group were lower than those of the control group, with statistically significant differences (P < 0.05). The results of inflammatory cytokine comparison within groups showed that compared with T1, at T2-T4 the levels of TNF-α, IL-6 and IL-10 significantly increased in the two groups (P < 0.05); the results of comparison among groups showed that at T1 there were no statistically significant differences in TNF-α, IL-6 and IL-10 levels between the two groups (P > 0.05), compared with the control group, at T2-T4 the levels of TNF-α and IL-6 significantly decreased and IL-10 levels significantly increased in the study group (P < 0.05). Conclusion The application of 0.5 μg/kg Dexmedetomidine in gynecological laparoscopic operation can reduce inflammatory cytokine levels, decrease inflammatory response, which is conducive to the operation and has great effects.
2017 Vol. 14 (28): 78-81 [Abstract] ( 286 ) HTML (1 KB)  PDF (456 KB)  ( 148 )
82 Application of acupuncture guided by Fuyang theory in cardiac rehabi?鄄litation
SHI Jiaheng1 LI Haixia2
Cardiac rehabilitation of integrated Chinese and western medicine is aimed at improving the quality of life in patients with cardiac diseases by various treatment methods of Chinese and western medicine. According to the characters of cardiac diseases, the Fuyang theory is put forward to restore the normal physiological function of human body by helping and adjusting Yang qi, which is consistent with the acupuncture mechanism of dredging the meridian, strengthening body resistance and eliminating evil, regulating Yin and Yang. This paper mainly expounds the application of acupuncture guided by Fuyang theory in cardiac rehabilitation from the origin of acupuncture, principle of acupuncture, Fuyang theory and the mechanism of acupuncture guided by Fuyang theory, in order to help patients with cardiac diseases restore health.
2017 Vol. 14 (28): 82-85,128 [Abstract] ( 231 ) HTML (1 KB)  PDF (1063 KB)  ( 244 )
86 Clinical effect of acupuncture combined with herb fumigation in the treatment of external hemorrhoids
HE Lifeng1 DONG Bo2 OU Guofeng3 LI Xiaoqun2 YUAN Puwei2
Objective To explore the clinical effect of acupuncture combined with herb fumigation in the treatment of external hemorrhoids. Methods The clinical data of 201 patients with external hemorrhoids in the Affiliated Hospital of Shaanxi University of Chinese Medicine, from January 2014 to December 2015 were reviewed. They were divided into acupuncture group, Chinese medicine group and acupuncture combined with Chinese medicine group according to therapies, with 67 cases in each group. 2 courses after treatment, the efficacy criteria, focusing on clinical symptoms and VAS score and recurrence after treatment, and comparative evaluation were compared. Results The total effective rate was 73.85% in the acupuncture group and 79.69% in the Chinese medicine group. The total effective rate was 91.15% in the acupuncture combined with Chinese medicine group. Comparison between acupuncture combined with Chinese medicine group and acupuncture group, the difference was statistically significant (P < 0.05) while there was no significant difference between Chinese medicine group and acupuncture combined with Chinese medicine group (P > 0.05). The VAS scores of the 3rd day, the 7th day and the 14th day were significantly lower than those before the treatment (P < 0.01), and the acupuncture combined with Chinese medicine group was significantly lower than the acupuncture group and the Chinese medicine group (P < 0.05). The patients were followed-up for 6,9,12 months after treatment. The recurrence rate of acupuncture group was lower than that of acupuncture group and Chinese medicine group, while the difference was not statistically significant (P > 0.05). Conclusion Acupuncture acupoints combined with traditional Chinese medicine is a good method to treat external hemorrhoids, which is effective and simple.
2017 Vol. 14 (28): 86-89 [Abstract] ( 240 ) HTML (1 KB)  PDF (821 KB)  ( 143 )
90 Current situation and development suggestions of traditional Chinese medicine industry in Pudong New Area
LI Huazhang1,2 LUO Zhiqin1 YU Donghai3 LIU Gang4 CAO Li4
With constant inclination and support from the national policy and the ongoing construction of a globally influential science and technology innovation center, the development of the traditional Chinese medicine industry in Pudong New Area has ushered in a new period of opportunities, forming the characteristics and advantages of strong research & development strength, prominent innovation achievements, public service platform aggregation and increasingly perfect innovation systems. On the basis of introducing the current development status of traditional Chinese medicine industry in Pudong New Area, this paper analyzes the existing problems of traditional Chinese medicine industry in Pudong New Area, including the insufficiency of the space for property management, the wide prevalence of the bottleneck of development fund, the weakness of traditional Chinese medicine industry service platform and specialized services, and the weakness of the overall strength of the district, so as to give recommendations including putting forward policies to support the industry, actively promoting the comprehensive support and pilot test, perfecting the multi-level system of talent introduction and cultivation and sounding multi-level and corresponding service platform system for future development.
2017 Vol. 14 (28): 90-92 [Abstract] ( 262 ) HTML (1 KB)  PDF (798 KB)  ( 93 )
93 Comparative study of the medical development history of Chinese and western "aromatherapy"
LI Zhiyue1 LI Feng1 ZHANG Yu1 SHAO Haozhen2 ZHANG Weiyue1 YU Jiaojiao1
"Aromatherapy" is originated from ancient civilizations - ancient Egypt and ancient China, which is originally used as customs and experiences in medicine; with the born of different historic cultures, philosophy and medicine in China and the West, Chinese and western "aromatherapy" becomes different: traditional Chinese medicine "aromatherapy" is classified into the category of external treatment of Chinese medicine - "aromatherapy therapy" and "fumigation therapy", it has been developed and matured in the aspects of drug type, dosage form, usage and theory, and has been used so far; western medicine "aromatherapy" has experienced the development and maturity of extracting essential oils, replacing essential oils by chemical synthesis, returning plant extracts and carrying out clinical research. There are such academic differences between Chinese medicine and western medicine "aromatherapy". In the final analysis, they are the differences of thinking patterns: the traditional Chinese medicine pays attention to the integrity of the body itself, and the unity and connection between human, nature and social environment; however, the reduction theory of western medicine divides the whole into several elements, and then makes a detailed analysis of the elements in order to trace the evidence and essence. To understand the similarity and difference of medical development paths between Chinese and western, and to seek common ground and fit points between them will bring about breakthroughs in the development and innovation of traditional Chinese medicine.
2017 Vol. 14 (28): 93-96 [Abstract] ( 329 ) HTML (1 KB)  PDF (817 KB)  ( 197 )
97 Clinical efficacy of traditional Chinese medicine combined with rectal ultrasound phoresis in the treatment of chronic non-bacterial prostatitis
WANG Peng WU Shaoqin HUANG Jie LONG Xiangqian LU Dandan
Objective To explore clinical efficacy of traditional Chinese medicine combined with rectal ultrasound phoresis in the treatment of chronic non-bacterial prostatitis. Methods From July 2013 to September 2014, 138 patients with chronic non-bacterial prostatitis in Jiangning TCM Hospital were divided into control group and observation group by random number table, with 69 cases in each group. The control group was treated with compound Chinese medicine blood stasis Sanjie blood activating agent, while the observation group was treated with traditional Chinese medicine combined with rectal ultrasound phoresis. Total efficiency, chronic prostatitis symptom (NIH-CPSI) score, TCM syndromes, urinary flow rate (UFR), residual urine volume (RVU), white blood cell count, interleukin-8 (IL-8) and tumor necrosis factor α (TNF-α) of patients in two groups were compared. Results ①Total efficiency in observation group after treatment was significantly higher than control group (P < 0.05). ②NIH-CPSI score and TCM syndromes scores in observation group after treatment were significantly lower than before treatment and control group (P < 0.05). ③MFR in observation group after treatment was significantly higher than before treatment and control group (P < 0.05). RVU and EPS-WBC in observation group after treatment were significantly lower than before treatment and control group (P < 0.05). ④The levels of IL-8 and TNF-α in observation group after treatment were significantly lower than before treatment (P < 0.05). And the level of IL-8 in observation group after treatment was significantly lower than control group (P < 0.05). Conclusion Traditional Chinese medicine combined with rectal ultrasound phoresis can improve clinical outcomes in patients with chronic non-bacterial prostatitis, reduce pain and voiding symptoms, improve quality of life for chronic nonbacterial prostatitis patients.
2017 Vol. 14 (28): 97-100 [Abstract] ( 194 ) HTML (1 KB)  PDF (751 KB)  ( 139 )
101 Study on Ge Shi Fang cited by Yi Xin Fang
XIAO Yongzhi HE Huiling
Yi Xin Fang was compiled by the Japanese medical home, Danbokonase, before the Chinese Song Dynasty. In the book, the citation indicates the source of the document, which includes a large collection of the original texts of Ge Hong's Ge Shi Fang in the Eastern Jin Dynasty of China. This paper starts from the provisions of distribution, the main way and main cure of syndrome, treatment characteristics, makes analysis and research of Ge Shi Fang cited by Yi Xin Fang preliminarily. According to the preliminary statistical analysis, among the 30 volumes of Yi Xin Fang, 26 volumes have cited the original texts of Ge Shi Fang; Ge Shi Fang cited by Danbo is the medical prescriptions of Ge Hong doctor that has not been cleaned up by the Jin-Dynasty scholar. Through the quote of Yi Xin Fang, the article reflects the characteristics of Ge's medical prescriptions, which is extensive, diverse, convenient to draw materials and easy to operate.
2017 Vol. 14 (28): 101-104 [Abstract] ( 196 ) HTML (1 KB)  PDF (825 KB)  ( 115 )
105 Analysis on the improvement effects of TCM syndrome differentiation + acupuncture for the clinical symptoms of herpes zoster neuralgia and its safety
CHEN Jingyao
Objective To observe the improvement effects of TCM syndrome differentiation + acupuncture for the clinical symptoms of herpes zoster neuralgia and its safety. Methods Ninety-eight cases with herpes zoster neuralgia admitted to Wangjing Hospital of China Academy of Chinese Medical Sciences from February 2014 to October 2016 were selected as research objects, and they were randomly divided into observation group and control group by random number table, with 49 cases in each group. Both groups were given routine treatment + TCM syndrome differentiation, on basis of which, observation group was added with acupuncture therapy. 7 d was as a course, for 2 courses. The clinical effects of the two groups were observed, the visual analogue scale (VAS) of the two groups before and after treatment were compared, the over time of herpes, escharosis time, the time of pain start to relieve, and the occurrence or not of adverse reactions in the two groups were recorded. All patients were followed-up for 3 months to observe the recurrence or not of neuralgia. Results The total effective rate of observation group was higher than that of control group, the difference was statistically significant (P ＜ 0.05). The VAS scores in the two groups after treatment were significantly lower than those before treatment (P ＜ 0.05), and the VAS scores in the observation group after treatment were significantly lower than those of control group, the difference was statistically significant (P ＜ 0.05). The over time of herpes, escharosis time, the time of pain start to relieve in the observation group was all shorter than that of control group, the differences were statistically significant (P ＜ 0.05). Followed-up for 3 months, both groups had no occurrence of neuralgia. There were no serious adverse reactions in the two groups during treatment. Conclusion TCM syndrome differentiation + acupuncture can improve the clinical symptoms of herpes zoster neuralgia effectively, and the safety is high, which is worthy of clinical promotion and application.
2017 Vol. 14 (28): 105-108 [Abstract] ( 233 ) HTML (1 KB)  PDF (812 KB)  ( 123 )
109 Clinical study on the treatment of YiQi Huoxue Decoction in senile patients with H type hypertension with syndrome of Qi deficiency and blood stasis
YU Rucui LU Zhihui
Objective To observe the effect of YiQi Huoxue Decoction on the blood pressure and homocysteine (Hcy) in senile patients with H type hypertension with syndrome of Qi deficiency and blood stasis. Methods This was arandomized single-blind control test. From January 2015 to December 2016, 60 senile patients with H type hypertension with syndrome of Qi deficiency and blood stasis in Anhui Provincial Hospital were selected and divided into control group (30 cases) and treatment group (30 cases), according to random number table. The control group was treated with conventional antihypertensive therapy, and the treatment group was treated with YiQi Huoxue Decotion on the basis of the control group. The antihypertensive effect, traditional Chinese medicine symptoms efficacy, traditional Chinese medicine symptom score and consulting room blood pressure (CBP)，ambulatory blood pressure (ABPM)，level of Hcy in the two groups four weeks after treatment were compared. Results The antihypertensive effect rate was 83.3% in the treatment group and 60.0% in the control group, with statistically significant difference (P < 0.05). The effective rate of Chinese medicine symptoms was 86.6% in the treatment group and 50.0% in the control group, with statistically significant difference (P < 0.05). Compared with before treatment, the CBP, ABPM significantly decreased in the control group after treatment (P < 0.05), excepted for Hcy and traditional Chinese medicine symptom score; and Hcy, CBP, ABPM and traditional Chinese medicine symptom score were significantly lower in the treatment group after treatment (P < 0.05); at the same time, the difference between two groups have statistical significance after treatment (P < 0.05). Conclusion YiQi Huoxue Decoction in the treatment of senile patients with H type hypertension with syndrome of Qi deficiency and blood stasis has obvious antihypertensive effect and clinical symptoms can be relieved.
2017 Vol. 14 (28): 109-112 [Abstract] ( 208 ) HTML (1 KB)  PDF (820 KB)  ( 162 )
113 Analysis of accuracy of preoperative MRI staging
LIN Weida LUO Yanxin
Objective To evaluate the accuracy of preoperative MRI staging in primary rectal cancer patients. Methods From December 2012 to April 2014, in Sixth Affiliated Hospital of Sun Yat-Sen University, 154 primary rectal cancer patients underwent MRI before surgery  were selected. The accuracy of preoperative MRI was analyzed by comparing with postoperative pathological stage. Results T stage was divided into T3-4 stage and T0-2 stage, the accuracy, sensitivity, specificity, PPV, NPV were 77.9%, 93.3%, 46%, 78.2%, 76.7%. N stage was divided into N+ stage and N0 stage, the accuracy, sensitivity, specificity, PPV, NPV were 57.1%, 78.2%, 45.5%, 44.3%, 78.9%. TNM stage was divided into Ⅱ-Ⅲ stage and 0-Ⅰ stage, he accuracy, sensitivity, specificity, PPV, NPV were 79.9%, 93.1%, 39.5%, 82.4%, 65.2%. Conclusion MRI can be applied as a useful tool in rectal cancer staging. Both T category and nodal metastasis has high sensitivity. But its low specificity may lead to over-staging.
2017 Vol. 14 (28): 113-116 [Abstract] ( 197 ) HTML (1 KB)  PDF (435 KB)  ( 201 )
117 Susceptibility-weighted imaging of clinical application of brain iron depositionin in patients with Parkinson disease
HE Juan1 Huriletemuer1 HAN Xiaodong2 SUN Ming1 ZHANG Chunyu1 DUAN Meiqing3 WANG Zhiguang1 ZHANG Guohua1 LIU Guiying1
Objective To clinical value of iron deposition in brain of Parkinson disease (PD) by susceptibility weighted imaging (SWI). Methods From June 2010 to June 2016, in Department of Neurology, Affiliated Hospital of Inner Mongolia Medical University, 40 PD patients were selected as PD group, 20 healthy volunteers were enrolled into the control group (NC group) at the same time. All PD patients were graded by Hoehn&Yahr, stage≤1.5 as early phase group (PD1 group) 23 cases, stage > 1.5 as middle and advanced phase (PD2 group) 17 cases. brain nuclei of each group were examinated by MRI and SWI with 3.0TMR system, and the results were compared and analyzed. Results The phase values of caudate nucleus (CN), globus pallidus (GP), putamen (PU), red nucleus (RN) and substantia nigra (SN) of patients in PD group increased compared with NC group, which indicated there was more iron deposits. The phase values of SN, GP and RN in PD1 group, PD2 group and NC group were compared, the differences were statistically significant (P < 0.05), but the phase values of PU, CN in three groups were compared, the differences were not statistically significant (P > 0.05); the phase values of SN, GP, CN in PD2 group were compared with NC group, the differences were statistically significant (P < 0.05), but these in PD1 group and PD2 group were compared, the differences were not statistically significant (P > 0.05). Conclusion SWI can reflect the iron deposition in the brain, and it can be used to evaluate the brain iron deposition by measuring the signal intensity of related brain nuclei and calculating the phase value. It can provide some help for diagnosis and treatment of the patients.
2017 Vol. 14 (28): 117-120 [Abstract] ( 223 ) HTML (1 KB)  PDF (767 KB)  ( 87 )
121 Application value of echocardiography in screening of cardiovascular disease in patients with chronic kidney disease
DONG Xin1 LI Renwu2 JI Limei2
Objective To investigate the value of echocardiography in screening of cardiovascular disease in patients with chronic kidney disease (CKD). Methods From September 2013 to July 2016, in Shunyi Hospital of Beijing Chinese Medicine Hospital ("our hospital" for short), 78 CKD patients were selected as the CKD group, and 78 healthy volunteers in our hospital were selected as the control group at the same period, the general survey data, echocardiography imaging and renal function index were recorded and given correlation analysis. Results There were no statistically significant differences in gender, age, systolic blood pressure, diastolic blood pressure, body mass index between the two groups (P > 0.05). The LAD, LVDd, LVDs, LVESV, LVEDV, E/A in the CKD group were (37.11±2.19) cm, (5.11±0.44) cm, (3.69±0.83) cm, (49.20±11.49) mL, (108.33±22.19) mL and (1.02±0.32), thoes in the control group were (34.11±3.10) cm, (4.72±0.44) cm, (2.93±0.44) cm, (25.02±5.66) mL and (76.20±13.10) mL and (1.27±0.44), there were statistically significant differences between the two groups in this index (P < 0.05). The Cr and BUN values of the CKD group were significantly higher than those of the control group, the differences were statistically significant (P < 0.05). There were no significant differences in UA, Hb, TG and LDL-C between the two groups (P > 0.05). In the CKD group, Pearson coefficient correlation analysis showed that LAD, LVDd, LVDs, LVESV, LVEDV were significantly positively correlated with Cr (r = 0.334, 0.514, 0.442, 0.298, 0.332, P < 0.05), E/A was negatively correlated with Cr (r = -0.345, P < 0.05), LAD, LVDd were positively correlation to BUN(r = 0.442, 0.301, P < 0.05). Multivariate regression analysis showed that LAD, LVDd, LVDs, E/A were the main independent risk factors for Cr (P < 0.05), LAD and LVDd were the main independent risk factors for BUN (P < 0.05). Conclusion The application of echocardiography in the screening of cardiovascular disease in patients with chronic kidney disease can effectively reflect the state of the heart function and the condition of the patients, and it is closely related to the routine indexes of renal function.
2017 Vol. 14 (28): 121-124,132 [Abstract] ( 254 ) HTML (1 KB)  PDF (649 KB)  ( 111 )
125 Curative efficacy of Weifuchun Tablets in combination with Esomeprazol in treating elderly patients with chronic atrophic gastritis and its effects on serum inflammatory factors
FAN Chunmei QIAO Jinwan
Objective To investigate the curative efficacy of Weifuchun Tablets in combination with Esomeprazol in treating elderly patients with chronic atrophic gastritis (CAG) and its effects on serum inflammatory factors. Methods From January to December 2016, 100 elderly patients with CAG in the Department of Gastroenterology, the Fifth People's Hospital of Qinghai Province were selected and divided into control group and observation group by random number table, with 50 cases in each group. Both groups were treated with routine therapy, besides, the control group was treated with Esomeprazole, and the observation group was treated with Weifuchun Tablets based on the control group therapy. The clinical efficacy of the two groups were observed. The levels of serum inflammatory factors between the two groups before and after treatment were compared. The adverse reactions were observed before and after treatment in two groups. Results The total effective rate of the observation group was significantly higher than that of the control group, with statistically significant difference（P < 0.05）.After treatment, the serum inflammatory factors (serum tumor necrosis factor alpha, interleukin 6, interleukin 8, etc.) of  two groups were significantly lower than before treatment, with statistically significant difference (P < 0.05). After treatment, the serum inflammatory factors in the observation group were significantly lower than those in the control group, with statistically significant difference (P < 0.05). There were no serious adverse reactions in the two groups of gastritis patients. There were no statistically significant differences at the incidence of adverse reactions in the two groups (P > 0.05). Conclusion Weifuchun Tablets combined with Esomeprazol has well curative efficacy in elderly patients with CAG. And it can increase therapeutic efficiency ratio and alleviate inflammatory reaction with not increasing incidence of adverse reactions.
2017 Vol. 14 (28): 125-128 [Abstract] ( 245 ) HTML (1 KB)  PDF (379 KB)  ( 75 )
129 Changes and significance of myocardial enzyme in the patients with acute cerebrovascular disease
REN Yongxia
Objective To investigate the changes and significance of myocardial enzyme in the patients with acute cere?鄄brovascular disease. Methods The clinical data of 68 patients with acute cerebrovascular disease (acute cerebrova?鄄scular disease group) admitted to People′s Hospital of Linyi City ("our hospital" for short) from August 2015 to December 2016 was analyzed retrospectively, 55 cases of healthy control in our hospital at the same time were selected as normal control group. The levels of aspartate aminotransferase (AST), lactic dehydrogenase (LDH), α-hydroxybutyrate dehydrogenase (α-HBDH), creatine kinas (CK), creatine Kinase Isoenzyme (CK-MB) were detected, and electrocardio?鄄gram was examined. Results The levels of AST, LDH, α-HBDH, CK in the acute cerebrovascular disease group were all higher than those of acute cerebrovascular disease group, the differences were all statistically significant (P < 0.05). There were no statistically significant differences of all indexes of myocardial enzyme between cerebral infarction group and cerebral ischemia group (P > 0.05). According to China stroke scale, the levels of CK in the medium group were signi?鄄ficantly higher than those of light group (P ＜ 0.05), the levels of all indexes of myocardial enzyme in the severe group were all higher than those of light group, the differences were all statistically significant (P ＜ 0.05). The levels of all indexes of myocardial enzyme in the non-conscious disturbance group were all lower than those of conscious disturbance group, the differences were all statistically significant (P ＜ 0.05). There were no statistically significant differences of all indexes of myocardial enzyme between normal electrocardiogram group and abnormal electrocardiogram group (P > 0.05). Conclusion Acute cerebrovascular disease can damage cardiac function, cause the changes of myocardial enzyme, which may be related to the severity of illness, conscious disturbance or not. Myocardial enzyme can be considered as the indexes of judging the severity degree of illness and prognosis of patients with acute cerebrovascular disease.
2017 Vol. 14 (28): 129-132 [Abstract] ( 186 ) HTML (1 KB)  PDF (358 KB)  ( 119 )
133 The value of general education in promoting the research study of medical students
ZHENG Xiang1 BI Wenjie2▲
General education which has a long history is a perfect form of education. But the effect of courses of general education are not ideal in medical education where there are strong professional factors and the students face the burden of hard work. This article analyzes the significance and conditions of carrying out general education in medical colleges and universities, based on the teaching experience of medical courses, student basic ability training and laboratory training, elaborates the concept and experience of decentralized fusion of general education. General education can be carried out in medical colleges and universities, and can promote the students' ability of research learning which is conducive to the future development of medical students.
2017 Vol. 14 (28): 133-136 [Abstract] ( 696 ) HTML (1 KB)  PDF (585 KB)  ( 90 )
137 Course design and practice of cell engineering for biotechnology
GAO Qiangguo YANG Jin LI Yuhong▲
Cell engineering is a required course for students major in biotechnology. To design a course of cell engineering that is suitable for the situation of Third Military Medical University, the authors have investigated on the course status of several universities and proposed the basic characteristics of cell engineering is high in technique, and large scale in content. The teaching thoughts of the course are thoroughly using the previous courses, making use of small class teaching and practical teaching. the authors have constructed the outline of teaching contents, including basic knowledge of cell engineering, animal cell culture, cell fusion, embryo engineering, chromosome engineering and stem cell engineering. Combined with the background of Third Military Medical University, the authors propose a set of effective course design, and take it into practice, in addition, summarize the teaching practice of cell engineering for biotechnology during these years, the major experiences are applying variegated teaching methods, small class teaching, training the scientific research and experimental abilities.
2017 Vol. 14 (28): 137-139 [Abstract] ( 201 ) HTML (1 KB)  PDF (607 KB)  ( 133 )
140 Reform and practice of Military Medical English Course from perspective of mutual integration
WANG Qingyi ZHOU Yumei
A blended learning model was designed to foster the professional English proficiency among militaries medical cadets in hope that they can become eligible military doctors capable of completing international exchanges and overseas missions. Micro-lectures, combined with classroom teaching and micro-lectures competitions, performed from the perspective of "mutual integration", they are the main features highlighted in this model. Feedbacks from two experimental classes, which included 80 students admitted in grade 2013 and grade 2014 respectively, they are collected after teaching-practice for two consecutive years. The results showed that this blended teaching approach could affect cadets cognition of their future jobs, reinforce their knowledge of the military medical care in foreign countries, and help them adapt to a renewed thinking pattern in the digital age.
2017 Vol. 14 (28): 140-143 [Abstract] ( 172 ) HTML (1 KB)  PDF (638 KB)  ( 191 )
144 Learning pathology and clinical practice in digital pathology times
FANG Wei CHEN Dong
The information technology has been developed rapidly, that led a huge transformation to many disciplines and research field. It is tend to combine information technology with different specialties. Traditional pathology is getting into digital time. Digital pathology brings so many differences in surgical pathology, teaching and scientific research, especially for the approaches of learning and teaching. This article discusses three contents in this review: digital virtual (slides) with pathology curriculum, three-dimensional print, virtual reality applying in anatomy and artificial intelligence in histology-from image analysis to automatic diagnosis. The prospect of application modern information technology to pathology would be analyzed.
2017 Vol. 14 (28): 144-147 [Abstract] ( 249 ) HTML (1 KB)  PDF (592 KB)  ( 146 )
148 Exploration and attempt of new teaching mode in oral medical professional English
LI Bo1 DONG Chunling2 YU Weixian1 GUO Xiaodong1 LI Baoquan1 LI Daowei1 SUN Hongchen1
Traditional professional English teaching in oral medicine can not meet the needs of international high-level medical talent under the new situation. It is necessary to change teaching thought, update teaching content and improve teaching quality. The new teaching mode is explored and practiced on several links including the focus on first class, novel teaching content, effective teaching method.
2017 Vol. 14 (28): 148-151 [Abstract] ( 219 ) HTML (1 KB)  PDF (586 KB)  ( 82 )
152 Teaching practice and exploration of pathological laboratory technology in higher vocational college
WANG Min1 LI Juntao1 ZHANG Xia1 LEI Weihua2
To meet the desperate need of high-quality pathological technicians in basic-level medical units, pathology laboratory technology has been opened in Zhaoqing Medical College for the students majoring in medical laboratory technology. In the course, various new teahing means and methods such as optimized the teaching contents, integrated on-and-off-campus resourses, module teaching, integration of theory and practice teaching, network teaching and opening laboratory, have greatly aroused the students′ interest in learning and effectively developed their practical ability, exploring ability, autonomous learning ability and communicative and cooperative ability. A systematic study of the course has enabled the students to master the basic knowledge and skills of pathology technology, and to strengthen their employ ability .    
2017 Vol. 14 (28): 152-154 [Abstract] ( 184 ) HTML (1 KB)  PDF (577 KB)  ( 88 )
155 Study on project risk management of hospital information system
WANG Hongyu1 LIU Chunxiu2
This article studied the general approaches of the risk management of hospital information system projects, aiming to provide methods of risk management focusing on important links of the process by analysing the domestic and foreign research data. Reference models and management processes of hospital information system project risk management are also put forward to discuss the components and relationship between models and processes. This research provides theoretical basis and reference models of risk management for developing and deploying hospital information system projects of China during the 13th Five-Year Plan period.
2017 Vol. 14 (28): 155-158 [Abstract] ( 183 ) HTML (1 KB)  PDF (518 KB)  ( 120 )
159 Effects of individualized nursing care on compliance behavior and nursing satisfaction in hypertensive cerebral hemorrhage patients with minimally invasive surgery
LIU Xin ZHANG Xiangtong
Objective To explore and analyze the effects of individualized nursing care on compliance behavior and nursing satisfaction in hypertensive cerebral hemorrhage patients with minimally invasive surgery. Methods Ninety cases of patients with hypertensive cerebral hemorrhage underwent minimally invasive surgery in the First Affiliated Hospital of Harbin Medical University from January 2015 to January 2017 were selected, and they were divided into control group and observation group by random number table, with 45 cases in each group. The control group was given routine nursing care, while the observation group was given individualized nursing care. Both groups were given continuous nursing for 1 month. The compliance behavior, nursing satisfaction and the incidence of complications between the two groups were compared. Results The total rate of compliance behavior in the observation group (93.33%) was higher than that of control group (75.56%), with statistically significant difference (P ＜ 0.05). The rate of nursing satisfaction in the observation group (91.11%) was higher than that of control group (73.33%), with statistically significant difference (P ＜ 0.05). The incidence of complications in the observation group (8.89%) was higher than that of control group (22.22%), with statistically significant difference (P ＜ 0.05). Conclusion The individualized nursing care can improve the compliance behavior of hypertensive cerebral hemorrhage patients with minimally invasive surgery, promote the nursing satisfaction, reduce the incidence of complications, and it is more safer.
2017 Vol. 14 (28): 159-161,169 [Abstract] ( 187 ) HTML (1 KB)  PDF (570 KB)  ( 111 )
162 Influence of attribution intervention basing on WeChat on self-management level of young and middle-aged patients with community hypertension
ZHANG Qing1 DING Fengmei2 BAO Jianli1 GAO Qiong1 LIN Cuixia1
Objective To explore the influence of attribution intervention on self-management level of young and middle-aged patients with community hypertension through WeChat. Methods From June to December in 2016, 100 young and middle-aged patients with community hypertension attended to community clinic in Zhucheng Ma Zhuang were  selected and divided into control group (50 cases) and observation group (50 cases) by random number table. Patients in control group were treated by routine nursing and health education, while patients in observation group were treated by attribution intervention through Wechat. Then the self-management ability and blood pressure level in the groups were compared. Results After the intervention, the self-management ability in observation group was higher than that in control group (P < 0.01), including the aspects of food management, emotional management, medication management, disease monitoring and exercise management. However, there was no statistically significant difference in the work and rest score between the groups (P > 0.05). In the blood pressure control, the scores of blood pressure in the observtion group were significantly lower than that in the control group(P < 0.01). Conclusion The implementation of attribution intervention through WeChat can improve patients′ self-management ability, help for blood pressure control, and improve the therapeutic effect and reuce the occurrence of complications.
2017 Vol. 14 (28): 162-165,173 [Abstract] ( 261 ) HTML (1 KB)  PDF (609 KB)  ( 236 )
166 The effect of continuous nursing on the recent recurrence and quality of life of patients  with non-small-cell lung cancer chemotherapy
SHEN Huimin1,2 LU Jibin1
Objective To explore and analyze the effect of continuous nursing on the recent recurrence and quality of life of patients with non-small-cell lung cancer chemotherapy. Methods From April 2015 to April 2016, 80 cases of patients with non-small cell lung cancer chemotherapy treated in Shengjing Hospital of Chinese Medical University were selected and divided into control group ( 40 cases) and observation group (40 cases) by random number table. The control group was treated by routine nursing, the observation group was treated by continuous nursing based on the control group. The follow-up lasted for 1 year, the recurrence, nursing satisfaction and quality of life in the two groups were observed. Results The recurrence rate in the observation group was lower than that in the control group, with statistically significant difference (P < 0.05). The nursing satisfaction in the observation group was higher than that in control group, with statistically significant difference (P < 0.05). After nursing, the scores of physical function, role function, cognitive function, emotional function and social function in the two groups was increased than before nursing, and the improvement of observation group was more remarkable than the control group, with statistically significant difference (P < 0.05). Conclusion The application of continuous nursing to patients with non-small cell lung cancer chemotherapy can reduce the recent recurrence rate, improve nursing satisfaction, and promote the improvement of quality of life.
2017 Vol. 14 (28): 166-169 [Abstract] ( 675 ) HTML (1 KB)  PDF (559 KB)  ( 87 )
170 Integrated care in the clinical applications of chemotherapy for advanced pancreatic cancer
XUE Xiaojing WANG Xiaozhen▲ DUAN Honghong WANG Wei XIE Jin
Objective To explore and analyze the clinical application of integrated care in the combination of advanced pancreatic cancer radiotherapy. Methods From April 2015 to April 2017, 90 patients with advanced pancreatic cancer underwent three dimensional conformal radiotherapy synchronous chemotherapy treatment in Xingtai City People's Hospital were selected and divided into control group and observation group by random number table, with 45 cases in each group. The control group was treated by the routine care, the observation group was treated by the comprehensive care, they were treated for two months. The adverse reaction, anxiety, depression and quality of life in the groups were compared. Results The observation group had a lower incidence of nausea and vomiting, diarrhea, constipation, liver and kidney dysfunction, abnormal blood picture, hair loss, and stomatitis compared with the control group, with statistically significant difference (P < 0.05). The SAS and SDS scores of the two groups after treatment were decreased than before treatment, the improvement of observation group was more remarkable, with statistically significant difference (P < 0.05). The physical function, role function, emotional function, cognitive function, social function and overall health in the observation group were higher than those in the control group, with statistically significant difference (P < 0.05). Conclusion The application of integrated care in advanced pancreatic cancer with radiotherapy and chemotherapy can reduce the incidence of toxic side effects, improve the anxiety and depression and improve the quality of life.
2017 Vol. 14 (28): 170-173 [Abstract] ( 163 ) HTML (1 KB)  PDF (587 KB)  ( 72 )
174 Effect of comprehensive nursing intervention combined with routine nursing care in patients after artery coronary bypass grafting
WANG Jing ZHANG Li▲
Objective To investigate the effect of comprehensive nursing intervention combined with routine nursing care in patients with artery coronary bypass grafting and its influence on prognosis. Methods From November 2014 to December 2016, 80 patients with artery coronary bypass grafting in the First Affiliated Hospital of Xinjiang Medical University were divided into control group (40 cases) and observation group (40 cases) by random number table. The control group received routine nursing care, while the observation group received comprehensive nursing intervention based on the control group. Hamilton anxiety scale (HAMA) and Hamilton depression scale (HAMD) were used to evaluate the psychological conditions of the two groups before and after nursing. Short form 36-item health survey (SF-36) was used to evaluate the quality of life in the two groups before and after nursing. The complications between the two groups were compared. Results Before nursing, there were no statistically significant differences of the scores of HAMA and HAMD between the two groups (P ＞ 0.05). After nursing, the scores of HAMA and HAMD were lower than those before nursing, and the observation group was reduced more significantly (all P ＜ 0.05). Before nursing, there were no statistically significant differences of the scores of SF-36 between the two groups (P ＞ 0.05), after nursing, the scores of SF-36 in the two groups were all higher than those before nursing, and the observation group was increased more significantly (all P ＜ 0.05). The incidence of postoperative complications in the observation group (10.00%) was lower than that of control group (17.50%), with statistically significant difference (P ＜ 0.05). Conclusion The effect of comprehensive nursing intervention combined with routine nursing care in patients with artery coronary bypass grafting is better, which can improve the prognosis of patients signficantly, and it is worthy of popularization and application.
2017 Vol. 14 (28): 174-177 [Abstract] ( 180 ) HTML (1 KB)  PDF (560 KB)  ( 131 )
178 Comparative study on the effects of three tube-sealing fluids for the tube-sealing of central venous catheters in patients with hemofiltration
JIN Yanhong YIN Lu
Objective To compare the effectiveness and safety of tube-sealing fluids of Sodium Heparin, Sodium Citrate and Urokinase for the tube-sealing of central venous catheters in patients with hemofiltration. Methods Sixty hemofiltration patients with central venous catheters admitted to Department of Critical Care Medicine, Beijing Friendship Hospital, Capital Medical University from May to October 2016 were selected, and they were divided into three groups by random table number, with 20 cases in each group. Group A was given Sodium Heparin to seal tube, group B was given Sodium Citrate to seal tube, group C was given Urokinase to seal tube. The effectiveness (including catheter jam, catheter dysfunction) and safety (including the incidence of gastrointestinal bleeding, drug allergy, catheter-related infection, hypocalcemia, errhysis around puncture point or subcutaneous hematoma) of the three groups after central vena catheterization for 21 d were compared. Results Among 60 patients, the incidence of catheter dysfunction (61.67%), errhysis around puncture point or subcutaneous hematoma (58.33%) was high and the incidence of drug allergy, catheter jam, catheter-related infection, gastrointestinal bleeding and hypocalcemia was low (＜5.00%). There were statistically significant differences in the incidence of catheter dysfunction and errhysis around puncture point or subcutaneous hematoma among the three groups (P ＜ 0.05). The incidence of catheter dysfunction in the group B and C was lower than that of group A, with statistically significant differences (P ＜ 0.05). The incidence of errhysis around puncture point or subcutaneous hematoma in the group B was lower than those of group A and C, with statistically significant differences (P ＜ 0.05). Conclusion The tube-sealing of Sodium Citrate in central venous catheters of patients with hemofiltration is more safe and effective, which is worthy of clinical popularization.
2017 Vol. 14 (28): 178-181 [Abstract] ( 264 ) HTML (1 KB)  PDF (728 KB)  ( 159 )
Copyright © Editorial Board of 中国医药导报
Supported by:Beijing Magtech