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| Analysis of Treg cells and lymphocyte subgroup in 131 patients with ankylosing spondylitis |
| WEI Yunyu1 HAN Zhijun1▲ HUANG Hongyu1 DU Wei2 REN Tianli3 GAO Mingzhu1 |
1.Department of Central Laboratory, Wuxi Second People′s Hospital of Nanjing Medical University, Jiangsu Province, Wuxi 214002, China;
2.Department of Orthopaedics, Wuxi Second People′s Hospital of Nanjing Medical University, Jiangsu Province, Wuxi 214002, China;
3.Department of Rheumatism, Wuxi Second People′s Hospital of Nanjing Medical University, Jiangsu Province, Wuxi 214002, China |
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Abstract Objective To analyze the changes of regulatory T cells (Treg cells) and the subsets of lymphocytes as well as the HLA-B27 expression in peripheral blood of ankylosing spondylitis (AS) patients, and then explore their roles in the development of AS. Methods Flow cytometry was applied. From February 2013 to December 2016, Department of Orthopedic and Rheumatology in Wuxi Second People's Hospital, 131 cases of AS patients were selected as AS group, 127 healthy subjects were selected as normal control group. The peripheral blood Treg cells, lymphocyte subsets and HLA-B27 were detected. The relationship of Treg cells, lymphocyte subsets, HLA-B27 and AS patients were analyzed. Results 131 cases of AS group, HLA-B27 positive for 123 cases (93.89%), the positive rate of HLA-B27 in normal control group was 2 cases (1.57%), the difference was statistically significant (P < 0.05). The positive rate of CD3+CD4+, CD4/CD8 and CD3-CD19+ in AS group were significantly higher than those in normal control group (P < 0.05). The positive rate of CD3+CD8+, CD16+/CD56+ in AS group were lower than those in normal control group (P < 0.05). The positive rate of CD4+CD25high and CD4+CD25highCD127low in AS group were lower than those in normal control group, the differences were statistically significant (P < 0.05). There was no significant difference in the positive rate of CD3+ and CD3+CD4+ between two groups (P > 0.05). Conclusion HLA-B27 has a strong correlation with AS. There are significant differences in the subgroups of lymphocytes in peripheral blood between AS and normal control group. At the same time, peripheral blood Treg cells in AS patients are significantly reduced. The above findings suggest that cellular immunity and humoral immunity may play an important role in AS.
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