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2021 Vol. 18, No. 33
Published: 2021-11-25
 
 
7 Study on the mechanism of Bushen Jiangzhi Formula regulating autophagy and cholesterol efflux on the intervention of atherosclerosis in ApoE-/- mice#br#
YAN Li SHEN Dingzhu
Objective To explore the mechanism of Bushen Jiangzhi Formula in regulating autophagy and cholesterol efflux in the intervention of atherosclerosis (AS). Methods One hundred ApoE-/- mice induced by high fat diet were divided into model group, kidney tonifying group, statin group and 3-methyladenine (3-MA) group by using random number table method, with 25 mice in each group. Twenty-five C57BL/6J mice were normal controls. Hematoxylin-eosin (HE), oil red O and FILIPIN staining were used to detect the pathological morphology, lipid accumulation and free cholesterol (FC) staining of the aortic sinus. Autophagosomes and lipid droplets were observed by electron microscopy. The content of serum total cholesterol (TC) and FC were detected by colorimetry. The expression of liver X receptor alpha (LXRα), ATP-binding membrane cassette transport protein A1 (ABCA1), microtubule-associated protein 1 light chain 3 (LC3) and p62 protein were detected by Western blot. Results The model group had more aortic sinus AS plaque, lipid accumulation and FC positive staining area than the normal group, and the differences were highly statistically significant (P ＜ 0.01). The normal group had intact aortic endothelial cell structure and autophagosome. The endothelial cells of aorta in model group were swollen with lipid droplets, but no autophagosomes were observed. The contents of TC, FC, and cholesterol ester (CE) in model group were higher than those in normal group, and the differences were highly statistically significant (P ＜ 0.01). The expression of LXRα, ABCA1, and LC3 Ⅱ/Ⅰ in aorta of model group was lower than that of normal group, and the expression of p62 was higher than that of normal group, the differences were highly statistically significant (P ＜ 0.01). AS plaque, lipid accumulation, and FC positive staining area of aortic sinus in the kidney tonifying group were less than those in the model group, and the differences were highly statistically significant (P ＜ 0.01). In the kidney tonifying group, autophagosomes were found in the endothelial cells of aorta. The contents of TC, FC, and CE in kidney tonifying group were lower than those in model group, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The expression of LXRα, ABCA1, LC3Ⅱ/Ⅰ in kidney tonifying group was higher than that in model group, while the expression of p62 was lower than that in model group, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Conclusion Bushen Jiangzhi Formula may effectively intervene in the treatment of AS by regulating autophagy and cholesterol efflux.
2021 Vol. 18 (33): 7-11 [Abstract] ( 94 ) HTML (1 KB)  PDF (1006 KB)  ( 65 )
12 Therapeutic effect of Shenyuan Yiqi Huoxue Capsules on cerebral thrombosis induced by Punatinib in zebrafish#br#
QIN Xiaomei1 GUO Shuaijie1 LI Yue1 LI Sinai1 LIU Weihong1 WANG Xuyun2 LIU Hongxu3 ZHOU Mingxue1▲
Objective To study the therapeutic effect of Shenyuan Yiqi Huoxue Capsules (hereinafte referred to as “Shenyuandan Capsules”) on cerebral thrombosis induced by Punatinib in zebrafish. Methods A total of 720 zebrfish were divided into four batches， 180 zebrfish were selected from each batch and placed in a six-well plate with 30 fish in each well. They were divided into six groups: normal control group, model group, positive control group and Shenyuandan Capsules low, middle, and high dose group, which were respectively given fish culture water, Prunatinib, Prunatinib and water-dissolved Aspirin (50 μg/ml) or 55.6, 167.0, 500.0 μg/ml Shenyuandan Capsules. After treatment for 18 h, the number of zebrafish forming cerebral thrombosis was observed; the kinematic velocity of zebrafish was measured by a behavioral analyzer; the blood flow video of zebrafish was recorded by the blood heartbeat analysis software to analyze the blood flow velocity of zebrafish; apoptosis number of brain cells in zebrafish was analyzed by acridine orange staining. Results Compared with model group, the incidence of cerebral thrombosis of zebrafish in Shenyuandan Capsules low, middle, and high dose groups were significantly decreased, and the differences were highly statistically significant (P < 0.01); the kinematic velocity of zebrafish in Shenyuandan Capsules low, middle, and high groups were significantly increased, and the differences were statistically significant (P < 0.01); blood flow velocity of zebrafish in Shenyuandan Capsules medium and high dose groups were significantly increased, and the differences were highly statistically significant (P < 0.01); apoptosis number of zebrafish brain cells in Shenyuandan Capsules high dose group were significantly decreased, and the difference was highly statistically significant (P < 0.01). Conclusion Shenyuandan Capsules can significantly improve the incidence of cerebral thrombosis and kinematic velocity induced by Pratinib in zebrafish, and the mechanism may be related to inhibiting brain cell apoptosis in zebrafish.
2021 Vol. 18 (33): 12-15,34 [Abstract] ( 182 ) HTML (1 KB)  PDF (709 KB)  ( 189 )
16 Construction of arsenic-resistant A216T missense mutant PML-RARα plasmid and the effect of realgar on its protein products and its mechanism#br#
PAN Yiming1 LI Ruibai1 HUANG Ziming1 LIU Yu1 FEI Mingming2 CHEN Lingyan2 HOU Li1
Objective To construct PMLA216T-RARα overexpression plasmid and to investigate the effect of realgar on its protein product and its mechanism. Methods The mRNA sequences of PML and RARα, and the fusion sites of PML-RARα were obtained from the database and literature. After designing primers, the target fragments were obtained by polymerase chain reaction, and the two fragments were joined by seamless cloning technique, inserted with mutations, and ligated with vector plasmids, and verified by sequencing. The plasmid was transfected into HL-60 cells, and the changes of PML-RARα, p-mTOR, p62, and LC3B levels were observed by Western blot after 24 h treatment with serum starvation method; and the changes of PML-RARα, p-mTOR, p62, and LC3B levels were observed by Western blot after 24 h treatment with different concentrations of realgar. Results The sequencing results showed that the plasmid was successfully constructed and the sequence conformed to the expected design. The levels of PMLWT-RARα and PMLA216T-RARα after 1, 2, 4, and 8 μmol/L realgar treatment were lower than those without realgar treatment (0 μmol/L), and the differences were statistically significant (P < 0.05); the level of PMLWT-RARα after 0.5 μmol/L realgar treatment was lower than that without realgar treatment, and the difference was statistically significant (P < 0.05). The level of PMLA216T-RARα was higher than that of PMLWT-RARα after the same realgar concentration treatment, and the difference was statistically significant (P < 0.05). The levels of PMLA216T-RAR α and p-mTOR in 5% FBS and untreated FBS were lower than those in 10% FBS, and the levels of p62 and LC3B were higher than those in 10% FBS, and the differences were statistically significant (P < 0.05). The levels of PMLA216T-RAR α after 4, 8, 16, 32, and 64 μmol/L realgar treatment were lower than those without realgar treatment, and the differences were statistically significant (P < 0.05); after 8, 16, 32, and 64 μmol/L realgar treatment, the levels of p-mTOR and p62 were lower than those without realgar treatment, and the LC3B level was higher than that without realgar treatment, and the differences were statistically significant (P < 0.05); the levels of p-mTOR and p62 after 16 μmol/L realgar treatment were lower than those after 8 μmol/L realgar treatment, and the LC3B level was higher than that after 8 μmol/L realgar treatment, and the differences were statistically significant (P < 0.05). Conclusion The missense mutant PMLA216T-RARα overexpression plasmid is successfully constructed, and high concentration of realgar may downregulate PMLA216T-RARα through selective autophagy.
2021 Vol. 18 (33): 16-20 [Abstract] ( 124 ) HTML (1 KB)  PDF (873 KB)  ( 90 )
21 Effects of low concentration bisphenol A on calcium spark of female adult rat cardiac myocytes and its mechanism#br#
YAN Sujuan1 CHENG Xiaoshu2 LI Juxiang2 CHEN Yamei3 DONG Min3 WANG Hongsheng3
Objective To investigate the effects and the mechanisms of bisphenol A (BPA) on calcium spark of the female adult rat cardiac myocytes. Methods Twenty healthy female SD rats weighing 200-250 g at 10-16 weeks old were selected and their cardiac myocytes were isolated in vitro after death. According to the experimental requirements, the cells were divided into control group and BPA group (10-9 mmol/L BPA was added). Cells in each group were obtained from at least three rats, and eight to ten cells were taken from each rat. Confocal microscope was used to detect calcium sparks in both groups, and calcium ion imaging system was used to detect calcium transients and calcium load of sarcoplasmic reticulum in two groups. Results Under confocal microscope, bright calcium sparks appeared in both groups. Calcium spark frequency and calcium transient peak value in BPA group were higher than those in control group, while calcium transient tau value was shorter than that in control group, the differences were highly statistically significant (P ＜ 0.01). There was no significant difference between the two groups in calcium spark peak value (P ＞ 0.05). In steady state, the calcium load of sarcoplasmic reticulum in BPA group was higher than that in control group, and the difference was statistically significant (P ＜ 0.05). There was no significant difference in tau value of sarcoplasmic reticulum calcium load between the two groups (P ＞ 0.05). At static state, there was no significant difference in the calcium load of sarcoplasmic reticulum between the two groups (P ＞ 0.05). Conclusion Under the action of BPA, the calcium load in the sarcoplasmic reticulum of female adult rat cardiomyocytes gradually increased with the contraction of cardiomyocytes, and the opening rate of renotin receptor also gradually increased, promoting Ca2+ leakage from the sarcoplasmic reticulum of cardiomyocytes during diastolic period, and increasing the frequency of calcium spark.
2021 Vol. 18 (33): 21-24 [Abstract] ( 67 ) HTML (1 KB)  PDF (695 KB)  ( 31 )
25 Network pharmacological prediction and molecular docking verification of the mechanism of Yueju Pills in the treatment of dyslipidemia#br#
CHANG Yan1,2 LIN Jianguo1 YAO Kuiwu1
Objective To analyze the mechanism of Yueju Pills in the treatment of dyslipidemia by network pharmacology and molecular docking. Methods The active components of Yueju Pills were searched by traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) and pertinent literatures, and their target genes were collected, the related gene targets of dyslipidemia were searched and screened by GeneCards, DrugBank and OMIM databases. Cytoscape 3.8.2 software was used to draw the network diagram of “active component-affect target” of Yueju Pills, and protein-protein interaction (PPI) network diagram was draw by STRING platform. The gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis of the drug-disease intersection targets were performed by Metascape database, and molecular docking was carried out by Autodock Vina. Results A total of 56 active components of Yueju Pills were collected, 113 drug-disease intersection targets were obtained, GO and KEGG enrichment analysis results showed that drug-disease intersection targets mainly enriched in cellular response to lipid, cellular response to hormones stimulus, cellular response to lipopolysaccharide and other biological processes, and mainly enriched in AGE-RAGE, TNF, PPAR, insulin, and other related signaling pathways. The results of molecular docking showed that the main active components of Yueju Pills in the treatment of dyslipidemia had strong affinity with the core targets. Conclusion Yueju Pills can correct body lipid metabolism disorder by reducing oxidative damage, promoting lipidemia metabolism, improving liver function, thus playing a role in dyslipidemia, which provides pharmacological basis for future clinical application and research.
2021 Vol. 18 (33): 25-30 [Abstract] ( 112 ) HTML (1 KB)  PDF (2157 KB)  ( 28 )
31 Mechanism of Huperzine A on encephalopathy in septic rats#br#
ZHOU Haiyin1 LONG Caixia1 LUO Lan1 CHEN Yanying1 LIU Pingping1 XIAO Zhenghui1 ZHANG Shuju2
Objective To investigate the protective effect of huperzine A (HupA) on septic encephalopathy in rats and the effect of autophagy. Methods A total of 60 Wistar rats were divided into three groups according to the random number table method: sham operation group (no cecal ligation and no cecal puncture), model group (modeled by cecal ligation perforation [CLP]) and treatment group (HupA+CLP), with 20 rats in each group. Biological signal recorder was used to monitor the vital signs (mean arterial pressure [MAP], heart rate [HR]) of rats 12 h after surgery. HE staining was used to observe the pathological changes of rat hippocampal tissue. The number of LC3 positive cells in hippocampal tissues of rats was observed by immunofluorescence. The expression of nuclear factor kappa-B(NF-κB) and autophagy-related proteins (LC3, Beclin1, LAMP-1, and Rab7) in hippocampus were detected by Western blot. Results The MAP of model group was lower than that of sham operation group, and the HR was higher than that of sham operation group, and the differences were statistically significant (P < 0.05). The MAP of the treatment group was higher than that of the model group, the HR was lower than that of the model group, and the differences were statistically significant (P ＜ 0.05). Hippocampal neurons in sham operation group and treatment group were basically normal, while hippocampal cells in model group were disordered with unclear structure and acute traumatic changes of neurons. The number of LC3 positive cells in the treatment group was higher than that in the model group, the difference was the difference were statistically significant (P ＜ 0.05). The expression levels of Beclin1, LAMP-1, and Rab7 in the model group were lower than those in the sham operation group, the expression levels of NF-κB and LC3 were higher than those in the sham operation group, and the expression levels of Beclin1, Rab7, and LAMP-1 in the treatment group were higher than those in the model group, the differences were statistically significant (P ＜ 0.05). Conclusion HupA inhibited NF-κB signaling pathway and promoted autophagy in hippocampus of sepsis rats, showing neuroprotective effects.
2021 Vol. 18 (33): 31-34 [Abstract] ( 84 ) HTML (1 KB)  PDF (707 KB)  ( 109 )
35 Research progress of autophagy in the prevention and treatment of malignant hematologic diseases#br#
WANG Wenru TANG Xudong
Autophagy can remove abnormal substances and organelles in cells in the early stage of malignant hematopathy, and help malignant cells to obtain various substances and energy in the later stage, and promote the growth and proliferation of tumor cells, which has a two-way effect. In clinical practice, chemoradiotherapy, demethylation, and targeted therapy can all activate autophagy, induce type Ⅱ programmed cell death of tumor cells and clear malignant cells, but a large amount of substances produced can help the survival of residual tumor cells and lead to drug resistance. It has been found that the combination of autophagy inhibitors can effectively improve the therapeutic effect of malignant hematologic diseases, especially for patients with relapsed and refractory diseases. In clinical treatment, how to effectively regulate the level of autophagy, remove tumor cells, and inhibit the progression of the disease are the key to the prevention and treatment of malignant hematologic diseases.
2021 Vol. 18 (33): 35-39 [Abstract] ( 151 ) HTML (1 KB)  PDF (596 KB)  ( 84 )
40 New advances in cognitive impairment associated with depressive disorder and its evaluation#br#
HAO Wensi1 WEI Min1 LIU Jianghong1 SONG Penghui1 WANG Jing2 WANG Yuping1
Depressive disorder is a common mental disorder characterized by emotional symptoms, such as low mood, slow thinking, and decreased will activity. More and more evidences indicate that the cognitive impairment in patients with depressive disorder is mainly related to the impairment of the frontal parietal cognitive control network. Of these, executive function, memory, and attention are the most commonly affected areas and can be assessed by specific neuropsychological tests. Executive function can be evaluated by trail making test part B, Stroop color-word test, etc. Memory is assessed by Wechsler memory scale and Rey auditory verbal learning test, and attention is assessed by continuous performance test, Delis-Kaplan executive function system test, CogitEx Ⅱ. At present, there are new comprehensive assessment methods for the cognitive function of patients with depressive disorder, such as THINC-it, severe cognitive impairment profile, cognitive component of rating bipolar disorder. A more detailed assessment of specific areas of cognitive impairment in patients with depressive disorders will improve the accuracy of screening and diagnosis, and have great implications for molecular studies and future targeted therapies.
2021 Vol. 18 (33): 40-44 [Abstract] ( 122 ) HTML (1 KB)  PDF (584 KB)  ( 144 )
45 Progress in the relationship between stem cell growth factors and tumor development#br#
XIE Qingqing1 SUN Yifan2▲
Hepatocyte growth factor (HGF) is a polypeptide growth factor with various biological activities, which is generally highly expressed in tumors. HGF exerts its effects in promoting tumor cell proliferation, inducing tumor cell invasion and metastasis by affecting apoptosis, regulating adhesion between cells, and promoting vascularization and lymphangiogenesis through the HGF/c-Met signaling pathway. In depth analysis of the status and role of HGF in tumor regulatory networks in different systems to uncover its molecular mechanism in tumorigenesis and development will provide new ideas for the diagnosis and treatment of cancer.
2021 Vol. 18 (33): 45-48,67 [Abstract] ( 66 ) HTML (1 KB)  PDF (892 KB)  ( 64 )
49 Progress in the treatment of polycystic ovary syndrome with spleen deficiency and phlegm dampness and insulin resistance#br#
QU Yunqing1 FENG Xiaoling2 HAN Zhiyu1
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women, moreover, insulin resistance (IR) plays a key role in the development of metabolic dysfunction, is the basis of obesity-related diseases such as PCOS. By optimizing your lifestyle and habits or taking drugs like Metformin, PCOS can be significantly improved, and IR can also be reversed to a large extent. According to Chinese medicine, PCOS belongs to the category of “late menstruation” and “amenorrhea”. It is caused by spleen deficiency and phlegm dampness. Invigorating the spleen and regulating qi, transporting phlegm and dampness. This paper reviews the progress of the treatment of spleen deficiency and phlegm dampness PCOS-IR by combining traditional Chinese medicine with Western medicine, in order to provide reference for clinical diagnosis and treatment.
2021 Vol. 18 (33): 49-52 [Abstract] ( 185 ) HTML (1 KB)  PDF (590 KB)  ( 118 )
53 Mechanism of pyroptosis and its role in various kidney diseases#br#
SUN Xiaoyi1 JIANG Yuqin1 TANG Yuyan2 LIU Hejing1 LU Xun3 WEI Minggang2
Pyroptosis is a newly discovered method of pro-inflammatory programmed cell death in recent years, which can be caused by inflammasome, caspase-1, human caspase-4/5, and mouse caspase- 11, and its downstream response factor gasdermin D (GSDMD) co-mediated. When the body is invaded by foreign substances, it can initiate the upstream reaction element of pyroptosis, which promotes the structural change of GSDMD. After more GSDMD N-terminal necrotic domains accumulate to form a membrane pore-forming active substance that causes cell membrane damage and causes inflammatory factors, such as interleukin-18 (IL-18) and IL-1β are released to cause inflammation. The mechanism of pyroptosis is involved in crystal-related nephropathy, ischemia-reperfusion kidney injury, diabetic nephropathy, and renal fibrosis. This article will review the specific mechanism of pyroptosis and its role in the occurrence and development of a variety of kidney diseases.
2021 Vol. 18 (33): 53-56 [Abstract] ( 99 ) HTML (1 KB)  PDF (739 KB)  ( 20 )
57 Analysis of the status quo and influencing factors of nursing research self-efficacy of nurses in district level hospitals#br#
ZHENG Jianing1,2 PENG Youqing3 HE Xiujun4 XIE Meng5 YAO Hongmei6
Objective To investigate the status quo of nurses’ self-efficacy in nursing research in district-level hospitals and to analyze influencing factors. Methods Cluster sampling was used to select 1075 nurses from eight district-level general hospitals in the three districts of Zhenhai, Beilun, and Yinzhou in six districts of Ningbo, Zhejiang Province from June to December 2020. The general information questionnaire and nursing research self-efficacy scale for investigation were used to survey. Results A total of 1075 questionnaires were distributed, and 1049 valid questionnaires were returned. The effective response rate was 97.58%. The total score of nursing research self-efficacy of survey subjects was 113.00 (56.50) points, and the score rate was 59.47%. The main ways most nurses choose to consult literature were Wanfang and the CNKI, which accounted for 30.03% and 26.11%, respectively. Multivariate linear regression analysis showed that educational background, professional title, receive scientific research course training, frequency of participating in scientific research lectures, literature reading time, publication of papers, and the entry of the subject into the regression equation were related factors that affected nurses’ research self-efficacy (P ＜ 0.05), it could explain 32.8% of the total variation. Conclusion The self-efficacy of nurse nursing research in district level hospitals needs to be improved. Nursing managers should strengthen nursing research training, formulate personalized and advanced research training programs that encourage nurses to participate in research activities, and enhance nurses’ research self-efficacy.
2021 Vol. 18 (33): 57-61 [Abstract] ( 215 ) HTML (1 KB)  PDF (654 KB)  ( 129 )
62 Analysis of the current status and influencing factors of treatment compliance of young and middle-aged patients with hypertension #br#
YOU Ziwei1 WU Lirong1 ZHOU Yang1 LIU Juan2 ZUO Guohua3 HU Hongjuan4
Objective To understand the current status of treatment compliance of young and middle-aged patients with hypertension and to analyze influencing factors. Methods From May to June 2020, a multi-stage sampling method was adopted to select a total of 227 young and middle-aged hypertensive patients managed by three community health service centers in Renmin Street, Yanfeng Street, and Lianhe Street, Hengyang City, Hunan Province as the research objects. The general information questionnaire, hypertension knowledge level scale, medical social support scale, hypertension self-care motivation subscale, hypertension treatment compliance scale were used to investigate the influencing factors of patients with hypertension treatment compliance. Results The treatment compliance score of young and middle-aged patients with hypertension was (93.00±11.66) points, which was at a low level. There were statistically significant differences in treatment compliance of young and middle-aged hypertensive patients with different genders, smoking, drinking, and comorbidities (P ＜ 0.05). Compliance with hypertension treatment was positively correlated with hypertension knowledge (r = 0.284), medical and social support (r = 0.379), and motivation for hypertension self-care (r = 0.570) (P ＜ 0.01). The results of multiple stepwise regression analysis showed that smoking, comorbidities, motivation for self-care of hypertension, and medical social support were related factors that affect patient compliance with treatment (P ＜ 0.05), which explained the 37.3% variation in hypertension treatment compliance. Conclusion The treatment compliance of young and middle-aged hypertensive patients is at a low level. Smoking, comorbidities, motivation for self-care of hypertension and medical social support are the influencing factors of treatment compliance of young and middle-aged hypertensive patients.
2021 Vol. 18 (33): 62-67 [Abstract] ( 229 ) HTML (1 KB)  PDF (574 KB)  ( 237 )
68 Self-concealment and influencing factors of patients with diabetic nephropathy on hemodialysis#br#
WU Yuanyuan TAO Rong XU Yaju ZHAO Xin
Objective To analyze the self-concealment and influencing factors of hemodialysis (HD) patients with diabetic nephropathy (DN). Methods Prospectively enrolled 100 patients with DN who received HD treatment from Maanshan Hospital of Traditional Chinese Medicine in Anhui Province from April 2019 to December 2020. The self-concealment scale (SCS) was used to assess the self-concealment of patients. The baseline data filling form, ask and record the relevant baseline data of patients were designed, the self-concealment status of patients with different data characteristics were compared, and the relevant factors that might affect the self-concealment status of HD patients with DN were analyzed. Results The self-concealment of 100 HD patients with DN was at a moderate level, and the SCS score was (32.80±2.22) points. There were statistically significant difference in self-concealment among patients with different dialysis age, self-esteem level, social support, and coping style (P ＜ 0.05). After multiple linear regression tests, dialysis age ≥ one year, low self-esteem, average social support, and negative coping were influencing factors for self-concealment tendency of HD patients with DN (P ＜ 0.05). The regression equation was as follows: Y = 5.992+4.244X1+9.698X2+5.889X3+10.401X4. Conclusion The self-concealment of HD patients with DN is at a moderate level. Dialysis age ≥one year, low self-esteem, average social support, and negative coping may be the main factors affecting the self-concealment of patients.
2021 Vol. 18 (33): 68-71 [Abstract] ( 379 ) HTML (1 KB)  PDF (553 KB)  ( 14 )
72 Related factors of autonomous perception level in patients with bronchial asthma#br#
LIU Xiaohui JIN Xiaoyan XIE Juyan
Objective To analyze the related factors of self-perceived level in patients with bronchial asthma (BA). Methods Prospectively included a total of 100 patients with BA who were treated in Hangzhou First People’s Hospital from January 2019 to January 2021 as the research objects. The autonomous perception scale was used to assess the autonomous perception level of all patients, and the baseline data statistics table was designed. The patients were asked in detail about the relevant baseline data and recorded, the scores of the autonomous perception scale of patients with different data characteristics were compared, and the relevant factors that may affect the autonomous perception level of BA patients were analyzed. Results The autonomous perception level of 100 patients with BA cases was average, and the autonomous perception score was (61.28±6.26) points. There was a statistically significant difference in the scores of the autonomous perception scale of patients with different education levels, other chronic diseases, anxiety and depression, and social support (P ＜ 0.05). Multiple linear regression analysis showed that low education, other chronic diseases, anxiety and depression, and low social support might be the influencing factors of BA patients’ perception of autonomy (P ＜ 0.05). The regression equation was as follows: Y = 48.745+2.693X1+1.250X2+1.379X3+1.932X4. Conclusion The autonomous perception level of BA patients may generally be related to low education, other chronic diseases, anxiety and depression, and low social support.
2021 Vol. 18 (33): 72-75 [Abstract] ( 340 ) HTML (1 KB)  PDF (552 KB)  ( 110 )
76 Cleaning effect and influencing factors of medical equipment in the central sterile supply department #br#
BAO Chun YAN Xing TIAN Lu
Objective To observe the cleaning effect of the central sterile supply department (CSSD) medical equipment, and to analyze the influencing factors of the unqualified cleaning. Methods A prospective sample selected 182 medical devices recovered by CSSD from Beijing Tongren Hospital Affiliated to Capital Medical University from January 2017 to December 2019. After cleaning, the cleaning effect of all medical devices and they were divided into qualified and unqualified groups according to the evaluation of cleaning effect. The baseline data questionnaire was designed, the influencing factors of CSSD medical device cleaning failure was analyzed. Results Ten of the 182 medical devices failed to be cleaned, with a failure rate of 5.49%, were regarded as the unqualified group; 172 medical devices were qualified, with a pass rate of 94.51%, which was regarded as the qualified group. There were statistically significant differences in the average daily frequency of use, equipment type, pollution degree, contaminated equipment placement time, lack of relevant knowledge of staff, and poor pretreatment of two groups (P ＜ 0.05). The logistic regression analysis showed that the average daily frequency of use (OR = 11.020), fine equipment (OR = 15.012), heavy pollution (OR = 31.652), long placement time of contaminated equipment (OR = 4.921), and the lack of relevant knowledge of staff (OR = 41.886) and poor pretreatment (OR = 15.657) were the influencing factors of CSSD medical device cleaning failure (P ＜ 0.05). Conclusion The rate of non-compliance of cleaning of medical devices in CSSD is higher, which may be related to the high daily use frequency of the equipment, fine equipment, heavy pollution, long placement time of contaminated equipment, lack of relevant knowledge of staff and poor pretreatment.
2021 Vol. 18 (33): 76-79 [Abstract] ( 123 ) HTML (1 KB)  PDF (381 KB)  ( 43 )
80 Meta-analysis of lncRNAs for diagnosis of type 2 diabetes mellitus#br#
TAN Xiaoyong1 ZHAO Jichang2 TIAN Mingying3 ZHAO Enhui3 ZHU Mei3 XING Yong1 RAN Qijun4
Objective To systematically evaluate the diagnostic value of long noncoding RNA (lncRNA) in type 2 diabetes by meta analysis. Methods PubMed, Embase, Cochrane Library, CNKI, Wanfang Data, and VIP were searched by computer, and clinical studies on lncRNAs in the diagnosis of type 2 diabetes mellitus published from inception to February 2021 were collected for screening, data extraction, and literature quality assessment. Meta-analysis was performed on the included studies by using Meta-disc 1.4 and Stata 16 software. Results A total of 12 studies were included, including 1776 patients with type 2 diabetes mellitus and 1508 healthy subjects. Meta-analysis results showed that the combination sensitivity was 0.75(95%CI: 0.73-0.77, P ＜ 0.001), the combination specificity was 0.70(95%CI: 0.67-0.72, P ＜ 0.001), and the area under the summary receiver operating characteristic curve was 0.79, Q* = 0.73. Subgroup analysis showed that the selection of lncRNAs with different variation trends was the source of heterogeneity in the diagnosis of type 2 diabetes mellitus (P ＜ 0.05). Sensitivity analysis results showed that the combined diagnostic odds ratio was 7.93 to 9.75 after the exclusion of literatures one by one, indicating that the included literatures had good stability. The results of publication bias analysis showed that there was no significant publication bias among the studies (P ＞ 0.05). Conclusion The expression level of circulating lncRNAs has important value and significance for the early diagnosis of type 2 diabetes mellitus.
2021 Vol. 18 (33): 80-85 [Abstract] ( 207 ) HTML (1 KB)  PDF (1192 KB)  ( 26 )
86 Correlation analysis between onset age of type 2 diabetes mellitus and late onset hypogonadism in male#br#
ZHAO Lei YUAN Mingxia LI Ruqiang
Objective To study the correlation between different onset ages of type 2 diabetes mellitus and late onset hypogonadism in male (LOH). Methods A total of 120 male patients with type 2 diabetes mellitus who were hospitalized in Beijing Friendship Hospital, Capital Medical University from October 2018 to March 2020 were selected. According to the age of onset of type 2 diabetes mellitus, they were divided into early onset group (＜ 40 years old, 54 cases) and late onset group (≥40 years old, 66 cases). The differences of clinical indicators between the two groups were collected and analyzed, and the correlation between total testosterone and age-related metabolic indicators was analyzed by Pearson and Spearman correlation coefficients. Results The disease course of the early onset group was shorter than that of the late onset group, the proportion of family history of diabetes, the proportion of new diabetes, body mass index, systolic blood pressure, glycosylated hemoglobin, fasting blood glucose, and triglyceride (TG) were higher than those of the late onset group, and high density lipoprotein cholesterol (HDL-C), follicle stimulating hormone, luteinizing hormone, the proportion of LOH were lower than those of the late onset group, the differences were statistically significant (P ＜ 0.05). Serum total testosterone level was negatively correlated with body mass index, fasting blood glucose, and TG (r or rs ＜ 0, P ＜ 0.05), but positively correlated with HDL-C (rs ＞ 0, P ＜ 0.05). Conclusion The onset age and related indexes of type 2 diabetes mellitus patients were correlated with LOH.
2021 Vol. 18 (33): 86-89 [Abstract] ( 120 ) HTML (1 KB)  PDF (468 KB)  ( 24 )
90 Effect of Levothyroxine Sodium on serum irisin level, blood lipid, and coagulation function of pregnant metaphase patients with subclinical hypothyroidism#br#
DENG Wenjuan1 NING Gaijun1 WANG Jing2 ZUO Lijuan1
Objective To study the effect of Levothyroxine Sodium on serum irisin level, blood lipid, and coagulation function of pregnant metaphase patients with subclinical hypothyroidism (SHT). Methods From June 2018 to June 2020, 80 pregnancy patients with SHT who received regular pregnancy checkups and perinatal care at the First Affiliated Hospital of Hebei North University were selected and divided into study group and control group by the random number table method, with 40 cases in each group. The control group was given regular diet guidance and other treatments, and the study group was given oral Levothyroxine Sodium Tablets. The levels of thyroid-stimulating hormone (TSH), free thyroxine (FT4), irisin, triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), prothrombin time (PT), activated partial thrombin time (APTT), fibrinogen were compared between the two groups before and after the treatment. Results After treatment, the levels of TSH, irisin, TG, TC, LDL-C, and fibrinogen in the study group were lower than before treatment, HDL-C was higher than before treatment, PT and APTT were longer than before treatment, and the levels of TSH, irisin, TG, TC, LDL-C, and fibrinogen in the study group were lower than those in the control group, while PT and APTT were longer than those in the control group, with statistical significances (P ＜ 0.05). Conclusion Levothyroxine Sodium can improve thyroid function and reduce serum irisin level in SHT patients during the pregnant metaphase, and also improve blood lipid and coagulation function to a certain extent.
2021 Vol. 18 (33): 90-93 [Abstract] ( 100 ) HTML (1 KB)  PDF (466 KB)  ( 70 )
94 Effect of Osteoking combined with closed reduction and percutaneous locking plate fixation on postoperative healing of tibial fracture#br#
LI Chaoyi WU Guozhi CHEN Rong ZHANG Wei ZHANG Peijun▲ TANG Jie
Objective To study the effect of Osteoking combined with closed reduction and percutaneous locking plate fixation (CRPLPF) on postoperative healing of tibial fracture. Methods From January 2017 to March 2019, 144 patients with middle and lower tibial fracture surgery in the Second Affiliated Hospital of Hainan Medical College were selected as research objects. According to the random number table method, they were divided into the control group and the observation group, with 72 cases in each group. The control group was treated with CRPLPF, and the observation group was treated with Osteoking on the basis of the control group. The detumescence time, fracture begin healing time, fracture healing time, serum insulin-like growth factor-1 (IGF-1), fibroblast growth factor-2 (FGF-2), bone gla-protein (BGP), bone alkaline phosphatase (BALP) levels, and hemorheology indexes of the two groups were observed. Results The observation group included 71 cases, and the control group included 69 cases. The postoperative detumescence time, fracture begin healing time and fracture healing time of the observation group were lower than those of the control group (P < 0.05). At four weeks after operation, the whole blood high shear viscosity, whole blood low shear viscosity, plasma viscosity, and erythrocyte aggregation index of the two groups were lower than those immediately after operation, and the observation group was lower than the control group (P < 0.05). At four weeks after operation, the levels of IGF-1, FGF-2, BGP, and BALP in the two groups were higher than those immediately after operation, and the observation group was higher than the control group (P < 0.05). The excellent and good rate in the observation group was higher than that in the control group (P < 0.05). Conclusion Application of Osteoking after CRPLPF can promote fracture healing and functional recovery.
2021 Vol. 18 (33): 94-98 [Abstract] ( 117 ) HTML (1 KB)  PDF (447 KB)  ( 33 )
99 Characteristics of multiple infection of human papillomavirus and its relationship with cervical lesions#br#
WANG Zici CHEN Xiaojing CHEN Jinjiao FAN Liangsheng WANG Wei XIAO Haiqun
Objective To investigate the characteristics of multiple infection of human papillomavirus (HPV) and its relationship with cervical lesions, which can provide references for the screening, treatment and vaccine development of cervical diseases. Methods The clinical data of 1175 patients with cervical pathology in the First Affiliated Hospital of Guangzhou Medical University from January 2014 to January 2020 were retrospectively analyzed. According to the different pathological diagnosis, they were divided into cervicitis group (107 cases), cervical intraepithelial neoplasia (CIN)Ⅰ group (364 cases), CIN Ⅱ-Ⅲ group (447 cases) and cervical cancer group (257 cases). The number of multiple infection of HPV cases, age distribution characteristics, infection type, and characteristics form of patients with different pathological types were compared. Results There were 291 cases of multiple infection of HPV, and the multiple infection rate was 24.77%. There were statistical differences in the incidence of multiple infection among different pathological types (P < 0.05). CIN Ⅰ was the predominant type of multiple infection of HPV in people under 30 years old, and CIN Ⅱ-Ⅲ was the predominant multiple infection of HPV in people after 30 years old. The highest HPV type detection rate of the first three kinds of subtypes were high-risk, respectively, HPV16, 52 and 58. Different cervical lesions were dominated by high-risk HPV mixed with other types of infection, and the proportion of double infection was the highest. Conclusion Multiple infection of HPV are found to be associated with the progression of cervical intraepithelial neoplasia, the prevention and treatment should be focused on patients under 30 years of age and patients with multiple HPV infections of HPV16, 52 and 58.
2021 Vol. 18 (33): 99-101,106 [Abstract] ( 164 ) HTML (1 KB)  PDF (567 KB)  ( 171 )
102 Clinical characteristics and risk factors of children with influenza A complicated with different complications#br#
LIU Yang1 WANG Aibin2 SONG Meihua2 WANG Caiying1 ZHANG Yanlan1 PANG Lin1▲
Objective To analyze the clinical characteristics and risk factors of children with influenza A complicated with different complications. Methods A retrospective analysis was performed on the clinical data of 1985 children under 18 years of age who were treated in the Infection Emergency Eepartment of Beijing Ditan Hospital Capital Medical University from December 2018 to February 2019, and the age distribution and clinical characteristics of children with influenza A complicated with different complications were analyzed, multivariate logistic regression was used to analyze the risk factors of different diseases associated with influenza A in children. Results Among the 437 children with influenza A complicated with other diseases, neurological diseases were the most common (10.8%), followed by pneumonia (8.0%) and bacterial infection (7.2%). The proportion of influenza-related complications was highest in children aged 1-3 years, few neurological complications occurred in children younger than 6 months, and the proportion of pneumonia was highest in children aged 1-2 years. Except for the complicated bacterial infection group, there were statistically significant differences in age distribution between the other complication groups and the simple influenza group (P < 0.05). The median age in the complication group was significantly lower than that in the simple influenza group, while the proportion of  boys, the time of first visit > 48 h, the time of first nueraminidase inhibitor (NAI) > 48 h, and complicated underlying diseases were significantly higher than those in simple influenza group (P < 0.05). Age, sex, underlying diseases, and time of first  NAI > 48 h were the risk factors of children with influenza complicated with different complications (OR > 1, P < 0.05). Compared with children aged 5-17 years, the risk of influenza-related complications in other age groups were significantly higher, and the risk of complications in children aged 1-3 years was 3.330 times higher than that in children aged 5-17 years. The risk of neurological complications in children aged 1-5 years was higher, and the risk of neurological complications in children with underlying diseases was 4.691 times higher than that in children without underlying diseases (P < 0.05). The risk of pneumonia was higher in 0-3 years old, male, complicated with underlying diseases and time of first NAI > 48 h (P < 0.05). However, influenza complicated with bacterial infection mostly occurred in children under 5 years old, combined with underlying diseases and time of first NAI > 48 h were independent risk factors (P < 0.05). Conclusion Nervous system diseases, pneumonia and bacterial infection are the most common complications of influenza in children. Male, children under 5 years old, complicated with underlying diseases and delayed antiviral treatment for more than 48 h after onset are the high risk factors of influenza complicated with other diseases.
2021 Vol. 18 (33): 102-106 [Abstract] ( 169 ) HTML (1 KB)  PDF (566 KB)  ( 38 )
107 Effect of Wujiajian Zhengqi Powder in the treatment of cold-damp type infantile rotavirus enteritis and its influence on serum myocardial enzyme spectrum and inflammatory factors#br#
WANG Zhenghua DING Pei XU Qiaowen ZHU Hui▲
Objective To explore the effect of Wujiajian Zhengqi Powder in treating cold-damp type infantile rotavirus enteritis and its influence on serum myocardial enzyme spectrum and inflammatory factors. Methods Eighty-eight cold-damp type infantile rotavirus enteritis admitted to Nantong Traditional Chinese Medicine Hospital of Jiangsu Province from June 2015 to June 2020 were selected as research subjects, and they were divided into control group (44 cases) and study group (44 cases) by random number table method. The control group was treated with conventional Western medicine, and study group was treated with Wujiajian Zhengqi Powder on the basis of control group. Both groups were treated for 14 days. The scores of cold-damp type Chinese medicine symptoms, serum creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), creatine kinase (CK), and tumor necrosis factor-α (TNF-α) levels were observed, and the clinical efficacy of two groups were compared, and the occurrence of adverse reactions during the treatment of two groups were observed. Results After treatment, cold-damp type Chinese medicine syndrome scores, CK-MB, LDH, CK, and TNF-α levels of two groups were lower than those before treatment, and study group was lower than control group (P ＜ 0.05). There was statistically significant difference in clinical efficacy between two groups (P < 0.05). There was no statistically significant difference in the incidence of adverse reactions between two groups (P > 0.05). Conclusion Wujiajian Zhengqi Powder combined with conventional Western medicine in the treatment of cold-damp type infantile rotavirus enteritis has definite curative effect, which can improve the serum myocardial enzyme spectrum, reduce inflammation, and is safe and reliable.
2021 Vol. 18 (33): 107-110 [Abstract] ( 204 ) HTML (1 KB)  PDF (540 KB)  ( 103 )
111 Effect of highly purified human menopausal gonadotrophin on in vitro fertilization in patients with diminished ovarian reserve at different ages#br#
ZHAO Haijun1 LU Jing1 ZHANG Yijiao1▲ WANG Yuhong1 CHEN Jing1 JING Li1 CHANG Yueting2 ZHANG Hongfeng3
Objective To investigate the effect of highly purified human menopausal gonadotropin (HP-hMG) on in vitro fertilization-embryo transfer in patients with diminished ovarian reserve (DOR) at different ages. Methods A total of 133 DOR patients from Handan Central Hospital of Hebei Province from July 2015 to December 2019 were selected, and they were divided into recombinant follicle stimulating hormone group (group A, 67 cases) and HP-hMG group (group B, 66 cases) according to treatment regimen; the two groups were further divided according to age (<35 years or ≥35 years), the ovarian response and pregnancy outcomes of the two groups at different ages were compared. Results Patients aged <35 years old: the amount of gonadotropin (Gn) in group B was higher than that in group A, and estrogen (E2) level on trigger day was lower than that in group A, with statistical significances (P < 0.05). There were no significant differences in cumulative pregnancy rate and cumulative live birth rate between two groups (P > 0.05). Patients aged ≥35 year old: the amount of Gn and top quality embryo rate in group B were higher than those in group A, E2 level on trigger day and egg number obtained were lower than those in group A, with statistical significances (P < 0.05). There were no significant differences in cumulative pregnancy rate and cumulative live birth rate between two groups (P > 0.05). Conclusion HP-hMG can significantly improve top quality embryo rate of DOR patients ≥35 years old.
2021 Vol. 18 (33): 111-114 [Abstract] ( 85 ) HTML (1 KB)  PDF (559 KB)  ( 26 )
115 Clinical effect of Liyan Kaiyin Decoction combined with conventional Western medicine in the treatment of vocal cord leukoplakia#br#
AN Jingjuan WU Kunmin▲ LI Zeqing ZHU Chunhui YANG Qi SHEN Jie FEI Youpeng WANG Lirong
Objective To evaluate the clinical efficacy of Liyan Kaiyin Decoction in the treatment of vocal cord leukoplakia. Methods A total of 69 patients with vocal cord leukoplakia admitted to the Jiangsu Second Hospital of Traditional Chinese Medicine from March 2017 to October 2019 were selected as the research objects. They were divided into study group (35 cases) and control group (34 cases) by random number table method. The control group received conventional Western medicine treatment, the study group received conventional Western medicine combined with Liyan Kaiyin Decoction treatment, and computer voice objective analysis was carried out. Objective voice, such as fundamental frequency perturbation (Jitter), amplitude perturbation (Shimmer), pitch perturbation quotient (PPQ), noise-to-harmonic ratio (NHR) and maximum phonation time (MPT), typing of intraepithelial papillary capillary loops (IPCL), clinical efficacy and adverse events were observed in two groups. Results  After treatment, the effective rate of study group was higher than that of control group (P ＜ 0.05). After treatment, there was a statistically significant difference in IPCL typing between the two groups (P ＜ 0.05). After treatment, Jitter, Shimmer, PPQ, NHR, and MPT in the two groups decreased compared with before treatment, and the observation group was lower than the control group, the differences were statistically significant (P ＜ 0.05). The incidence of adverse events in the study group was lower than that in the control group, the difference was statistically significant (P ＜ 0.05). Conclusion Liyan Kaiyin Decoction is effective in treating vocal cord leukoplakia and improving voice after objective voice evaluation.
2021 Vol. 18 (33): 115-118 [Abstract] ( 128 ) HTML (1 KB)  PDF (374 KB)  ( 26 )
119 Discussion on the pathogenesis of Chaihu Guizhi Ganjiang Decoction in Treatise on Cold Pathogenic Diseases based on quantitative analysis#br#
WU Qianting1 LIU Min2
Objective To quantitatively analyze the pathogenesis characteristics of Chaihu Guizhi Ganjiang Decoction. Methods The specific composition and dosage of classical prescriptions in Treatise on Cold Pathogenic Diseases were summarized, and the same drugs with different treatment dosage and processing methods were distinguished. The efficacy weight of each drug was calculated by double-layer frequency weight cross algorithm, and then the efficacy weight of Chaihu Guizhi Ganjiang Decoction was calculated in order to judge its efficacy level, so as to analyze its law of concerted application and finally deduce the pathogenesis characteristics. Results In Chaihu Guizhi Ganjiang Decoction, the level Ⅲ efficacies were purging gallbladder, activating qi, tonifying heart, treating palpitations, and relieving pain;the level Ⅱ efficacies were clearing heat, resolving masses, dissipating phlegm, and relieving cough; the level Ⅰ efficacies were dispersing liver, warming spleen, moistening lung, relieving superficies, eliminating carbuncle sore, and preventing malaria; the level -Ⅰ efficacies were clearing heart, suppressing hyperactive liver, eliminating dampness, inducing diuresis, relieving sore throat, and stopping bleeding. Conclusion The core pathogenesis of Chaihu Guizhi Ganjiang Decoction is Shaoyang cardinal disadvantage, gallbladder fire depression, qi and body fluid movement disorder, combined with taiyin spleen cold; Secondary pathogenesis includes body fluid injury and solar surface syndrome.
2021 Vol. 18 (33): 119-122 [Abstract] ( 78 ) HTML (1 KB)  PDF (687 KB)  ( 91 )
123 Visual analysis of domestic research status of Aflatoxin in traditional Chinese medicine based on CiteSpace#br#
YE Yaomin LEI Houxing ZHANG Xiaoqin LIN Na
Objective To analyze the research status of Aflatoxin in traditional Chinese medicine in China. Methods Literatures related to Aflatoxin research published on China National Knowledge Internet from January 1981 to December 2020 were searched, and CiteSpace software was used for bibliometric analysis of 327 included literatures. The visual network showed the knowledge graph constructed by authors, publishing institutions and keywords. Results From 2014 to 2020, the number of literatures published on Aflatoxin in traditional Chinese medicine showed a wave upward trend. Yang Meihua published the most articles, and Hangzhou Institute of Food and Drug Control published the most articles. The development trend of Aflatoxin research was Aflatoxin content detection method; the hot topic was the rapid detection of Aflatoxin in traditional Chinese medicine and Chinese patent medicine. Conclusion This paper provides scientific basis for the follow-up study of Aflatoxin.
2021 Vol. 18 (33): 123-126,160 [Abstract] ( 149 ) HTML (1 KB)  PDF (1513 KB)  ( 124 )
127 Clinical effect of modified Linggui Zhugan Decoction combined with Metformin in the treatment of obesity type 2 diabetes mellitus with phlegm-dampness syndrome#br#
LUO Li1 LIU Chungu1 HUANG Yu2 ZHANG Zhenyu2 YU Yaohe1 LONG Shuni1
Objective To observe the clinical effect of modified Linggui Zhugan Decoction combined with Metformin in the treatment of obesity type 2 diabetes mellitus (T2DM) with phlegm-dampness syndrome. Methods Seventy obesity T2DM patients with phlegm-dampness syndrome in Guangzhou Xinhai Hospital from February 2018 to May 2021 were selected and divided into control group and experimental group by random number table method, with 35 cases in each group. All patients were treated with health education and lifestyle intervention. The control group were treated with Metformin, and the experimental group were treated with modified Linggui Zhugan Decoction combined with Metformin. Both groups were treated for six weeks. The changes of body mass index (BMI), waist-to-hip ratio (WHR), fasting blood glucose (FPG), 2 hours postprandial blood glucose (2hPG), glycosylated hemoglobin (HbA1c), triglyceride (TG), and low density lipoprotein cholesterol (LDL-C) in two groups were compared before and after treatment, and the clinical efficacy of the two groups was evaluated. Results After treatment, BMI, WHR, FPG, 2hPG, HbA1c, TG, and LDL-C in two groups were lower than before treatment, and FPG, 2hPG, HbA1c, TG, and LDL-C in the experimental group were lower than those in the control group (P ＜ 0.05). After treatment, the clinical effect of the experimental group was better than the control group (P ＜ 0.05). Conclusion The clinical efficacy of modified Linggui Zhugan Decoction combined with Metformin in the treatment of obesity T2DM patients with phlegm-dampness syndrome is superior to that of Metformin alone, and it has advantages in regulating glucolipid metabolism disorder, which is worthy of clinical promotion.
2021 Vol. 18 (33): 127-130 [Abstract] ( 110 ) HTML (1 KB)  PDF (688 KB)  ( 82 )
131 Meta analysis of clinical effect of Zhenwu Decoction on diabetic kidney disease#br#
CHEN Shifei GAO Fangying▲ LU Wen SUN Chengli TAO Huilin
Objective To systematically evaluate the clinical effect of Zhenwu Decoction on diabetic kidney disease. Methods CBM, China National Knowledge Internet, Wanfang, VIP, PubMed, Cochrane Library and EMbase databases were searched for randomized controlled trials of Zhenwu Decoction in the treatment of diabetic kidney disease. The retrieval period is from inception to November 2020 of each datobse. Quality evaluation and meta-analysis were performed using RevMan 5.3 software. Results A total of 11 randomized controlled trials involving 838 diabetic kidney disease patients were included. Meta-analysis results showed that the total effective rate of the experimental group (OR = 4.62, 95%CI: 3.05 to 7.02, P ＜ 0.05) was higher than that of the control group, and the difference was statistically significant (P ＜ 0.05). The 24 h urine protein quantification (MD = -0.73, 95%CI: -0.90 to -0.57, P < 0.05), serum creatinine (MD = -16.61, 95%CI:-17.10 to -16.12, P ＜ 0.05), fasting blood glucose (MD = -0.42, 95%CI: -0.61 to -0.23, P ＜ 0.05) in the experimental group were lower than those in the control group, and the differences were statistically significant (P ＜ 0.05). Conclusion Zhenwu Decoction combined with conventional Western medicine can effectively treat diabetic kidney disease.
2021 Vol. 18 (33): 131-134 [Abstract] ( 392 ) HTML (1 KB)  PDF (1415 KB)  ( 36 )
135 Effect of Jianpi Qingre Shengjiang Decoction combined with Western medicine on reflux esophagitis#br#
SHENG Zhenxing1 WANG Yizhou2 REN Lijun3▲
Objective To investigate the effect of Jianpi Qingre Shengjiang Decoction combined with Western medicine on reflux esophagitis. Methods A total of 80 cases of reflux esophagitis patients with syndrome of qi inversion in middle deficiency admitted to Anhui Wuhu Chinese Medicine Hospital from April 2019 to July 2020 were selected and divided into combined group and control group according to random number table method, with 40 cases in each group. The control group was treated with conventional Western medicine, and the combined group was treated with Jianpi Qingre Shengjiang Decoction combined with Western medicine. Both groups were treated for eight weeks. Traditional Chinese medicine symptom score, clinical efficacy, serum gastrin (GAS) and plasma pepsin (PG) Ⅰ and Ⅱ levels and adverse reactions were compared between the two groups. Results There was no significant difference in traditional Chinese medicine symptom score between the two groups before treatment (P ＞ 0.05). After treatment, the scores of traditional Chinese medicine symptoms in both groups were higher than those before treatment, and the combined group was higher than the control group, the differences were statistically significant (P ＜ 0.05). After treatment, the total effective rate of combined group was higher than that of control group, the difference was statistically significant (P ＜ 0.05). There were no significant differences in serum GAS and plasma PG Ⅰ and PG Ⅱ levels between the two groups before treatment (P ＞ 0.05). After treatment, the levels of serum GAS and plasma PG Ⅰ in both groups were higher than those before treatment, and those in the combined group were higher than those in the control group. The levels of plasma PG Ⅱ in both groups were lower than those before treatment, and those in the combined group were lower than those in the control group, with statistical significances (P ＜ 0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P ＞ 0.05). Conclusion The combination of Jianpi Qingre Shengjiang Decoction combined with Western medicine in the treatment of reflux esophagitis can improve the clinical symptoms, with definite curative effect, and improve serum GAS and plasma PG levels, which is safe and reliable.
2021 Vol. 18 (33): 135-138 [Abstract] ( 69 ) HTML (1 KB)  PDF (641 KB)  ( 25 )
139 Clinical effect of Dingchuan Decoction in the treatment of asthma-chronic obstructive pulmonary disease overlap syndrome with syndrome of phlegm heat obstructing lung#br#
LI Yue1 ZHENG Lan1 GAO Yue2
Objective To investigate the clinical effect of Dingchuan Decoction in the treatment of asthma-chronic obstructive pulmonary disease overlap syndrome (ACOS) with syndrome of phlegm heat obstructing lung. Methods A total of 95 ACOS patients with syndrome of phlegm heat obstructing lung admitted to Hubei Provincial Hospital of TCM from January 2018 to September 2020 were selected. According to random number table method, they were divided into control group (47 cases) and study group (48 cases). The control group was treated with routine treatment and Salmeterol Xinafoate and Fluticasone Propionate Powder for Inhalation, and the study group was treated with Dingchuan Decoction on the basis of control group. Both groups were treated for 3 months. The clinical efficacy, inflammatory factor, lung function, clinical symptom score and incidence of adverse reactions were compared between the two groups. Results After treatment, the total effective rate in the study group was higher than that in the control group (P ＜ 0.05). After treatment, interleukin-6, interleukin-10, and high-sensitivity C-reactive protein in the two groups were lower than those before treatment, and study group was lower than control group (P ＜ 0.05). After treatment, forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), and FEV1/FVC in the two groups were higher than those before treatment, and study group was higher than control group (P ＜ 0.05). After treatment, asthma control test scores in the two groups were higher than those before treatment, and the study group was higher than the control group (P ＜ 0.05); chronic obstructive pulmonary disease assessment test scores in the two groups were lower than those before treatment, and the study group was lower than the control group (P ＜ 0.05). After treatment, there was no significant difference in the incidence of adverse reactions between the two groups (P ＞ 0.05). Conclusion Dingchuan Decoction has a significant curative effect in the curative of ACOS with syndrome of phlegm heat obstructing lung, which can effectively improve the clinical symptoms, lung function and inflammatory factor levels of patients, with good safety and high clinical application value.
2021 Vol. 18 (33): 139-142,168 [Abstract] ( 173 ) HTML (1 KB)  PDF (1078 KB)  ( 50 )
143 Study on the medication law of traditional Chinese medicine in the treatment of membranous nephropathy based on data mining#br#
WANG Rumeng ZHAO Mingming YU Yi CHANG Meiying MA Sijia ZHANG Yu
Objective To explore the medication law of traditional Chinese medicine in the treatment of membranous nephropathy. Methods Periodical literatures collected by CNKI were used as data sources. The literature studies about traditional Chinese medicine in the treatment of membranous nephropathy from January 2000 to September 2020 were collected and summarized. Excel 2013, SPSS Modeler 18.0 and SPSS Statistics 21.0 software were used, and the frequency of drug use, nature and flavour, channel tropism, related association rule and clustering results were used as observation indexes for statistical analysis. Results Through screening, 72 formulas and 116 kinds of herbs were obtained. Among them, 26 herbs were used at least 10 times, four nature of drugs were mainly warm, cold and calm, five flavours were mainly sweet, bitter and spicy, the mainly meridians were kidney meridian, lung meridian, spleen meridian and liver meridian. Radix Astragali seu Hedysari, Poria, Radix Salviae Miltiorrhizae, Rhizoma Atractylodis Macrocephalae, Radix Angelicae Sinensis were commonly used. Four cluster formulas were obtained by high-frequency drug clustering, which showed that traditional Chinese medicine in the treatment of membranous nephropathy by using the therapies of dissipating wind and unblocking collaterals, activating blood and resolving stasis, resolving dampness and disinhibiting water, clearing heat and removing toxin, and others on the basis of tonifying spleen and kidney to syndrome differentiation. Conclusion Traditional Chinese medicine in the treatment of membranous nephropathy follows the pathogenesis nature of “root deficiency and manifestation excess”. Medications should pay attention to the application of both warming and clearing therapies, reinforcing and reducing. Kidney, lung and spleen are regulated simultaneously and paid attention, and other organs are gave consideration. The summary of the characteristics of modern doctors in the treatment of membranous nephropathy has reference significance for the clinical treatment of membranous nephropathy.
2021 Vol. 18 (33): 143-146 [Abstract] ( 138 ) HTML (1 KB)  PDF (945 KB)  ( 164 )
147 Effect of individualized psychological intervention in cesarean section perioperative period and its influence on self-efficacy#br#
MU Na1 SHI Xiaoli2 WANG Shihong3
Objective To explore the effect of individualized psychological intervention in cesarean section perioperative period and its influence on self-efficacy. Methods From January 2019 to January 2020, 266 women who underwent cesarean section in Shandong Rizhao Maternal and Child Health Hospital were selected as the research objects, they were divided into control group and observation group by random number table method, with 133 cases in each group. The control group received routine perioperative nursing, while the observation group received individualized psychological intervention. Self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were used to evaluate and compare the psychological mood of the two groups before and after intervention, visual analogue scale (VAS) was used to compare the pain degree of the two groups at 12 and 24 h after intervention, general self-efficacy scale (GSES) was used to compare the self-efficacy of the two groups after intervention, operation time, postoperative exhaust time and hospital stay were compared between the two groups, the incidence of postoperative complications such as abdominal distention and urinary retention was compared between the two groups. Results After intervention, SAS and SDS scores of the two groups were lower than those before intervention, and observation group was lower than control group, and the differences were statistically significant (P ＜ 0.05). After intervention, VAS score of observation group was lower than that of control group, GESE score was higher than that of control group, and the differences were statistically significant (P ＜ 0.05); the operation time, postoperative exhaust time and hospital stay in the observation group were shorter than those in the control group, and the differences were statistically significant (P ＜ 0.05); after intervention, the total incidence of complications in the observation group was lower than that in the control group, and the difference was statistically significant (P ＜ 0.05). Conclusion Individualized psychological intervention during the perioperative period of cesarean section can enhance maternal self-efficacy, improve maternal bad mood, contribute to improve the recovery after childbirth and obstetrics, which is worthy of clinical reference.
2021 Vol. 18 (33): 147-150 [Abstract] ( 278 ) HTML (1 KB)  PDF (628 KB)  ( 44 )
151 Meta analysis of the effect of self-management intervention in elderly patients with urinary incontinence#br#
ZHANG Liya1 REN Pengna2 LIN Mengyue2 HU Xiaoyi3 TANG Ailing3 ZHANG Yue3 XU Fanglei3
Objective To systematically evaluate the effect of self-management intervention in elderly patients with urinary incontinence. Methods A computer search of Embase, PubMed, Web of Science, Wanfang, CNKI, VIP and China Biomedical Literature Database were conducted to collect randomized controlled trials on the application of self-management intervention in elderly patients with urinary incontinence from the establishment of the database to December 2020. The relevant indexes included in the literature were analyzed by meta with RevMan 5.3 software. Results A total of 697 patients were included in 10 literatures. The results of meta analysis showed that the incidence of urinary incontinence in the intervention group was lower than that in the control group (MD = -4.53, 95%CI: -5.63, -3.43, P ＜ 0.000 01), and the quality of life (MD = 12.14, 95%CI: 2.60, 21.68, P = 0.01), self-efficacy (MD = 20.14, 95%CI: 0.39, 39.89, P = 0.05) and knowledge level of urinary incontinence (MD = 7.2, 95%CI: 1.38, 13.01, P = 0.02) were higher than those in the control group. Conclusion Self-management intervention can reduce the incidence of elderly patients with urinary incontinence, improve the quality of life and improve self-efficacy, but limited by the number and quality of studies, large samples and high-quality studies are still needed toverify its long-term effect.
2021 Vol. 18 (33): 151-155 [Abstract] ( 159 ) HTML (1 KB)  PDF (1278 KB)  ( 127 )
156 Research hotspots and development trend of traditional Chinese medicine nursing research of tumor patients based on CiteSpace#br#
XIAO Chun1 LI Wenya2 LIU Yajian2 PENG Xiaoling3
Objective To understand the research status, hot spots and development trend in the field of traditional Chinese medicine oncology nursing in China, and provide reference for future research. Methods Literatures on traditional Chinese medicine oncology nursing from CNKI, Wanfang and VIP from January 1996 to May 2021 were searched, CiteSpace 5.7 R1 software was used to visually analyze the number of articles, authors, institutions, and keywords. Results After retrieval and screening, 904 articles were included, and the number of articles presented an upward trend, the research hotspots mainly include chemotherapy, emotional nursing, quality of life, etc. Research frontiers include sleep quality, cancer-related fatigue, and negative mood. Conclusion It is necessary to strengthen cooperation among traditional Chinese medicine tumor nursing teams and institutions, and carry out more interdisciplinary high-quality research to provide evidence for clinical practice.
2021 Vol. 18 (33): 156-160 [Abstract] ( 157 ) HTML (1 KB)  PDF (1328 KB)  ( 32 )
161 Effect of IMB mode nursing intervention on quality of life of patients with indwelling PICC#br#
LI Xinru ZHOU Tingting ZHOU Jing
Objective To analyze the effect of nursing intervention of information-motiveation-behavioral skills model (IMB) on quality of life of patients with peripherally inserted central catheter (PICC). Methods A total of 120 tumor patients treated in Huaian First People’s Hospital of Jiangsu Province from February 2020 to February 2021 were selected, they were divided into control group and experimental group by random number table method, with 60 cases in each group. Negative psychological status, quality of life, adherence to catheter maintenance and incidence of complications were compared between the two groups. Results After treatment, anxiety and depression scores of the two groups were lower than before those treatment, and the experimental group was lower than the control group, and the differences were statistically significant (P ＜ 0.05); the scores of physical function, social function, psychological function, and material life of the two groups were higher than those before treatment, and the experimental group was higher than the control group, and the differences were statistically significant (P ＜ 0.05); the adherence rate of catheter maintenance in the experimental group was higher than that in the control group, the difference was statistically significant (P ＜ 0.05); the incidence of total complications in the experimental group was lower than that in the control group, and the difference was statistically significant (P ＜ 0.05). Conclusion The application of IMB mode nursing intervention in patients with PICC catheterization can improve patients’ compliance with catheterization and self-care ability, relieve adverse emotions, reduce the occurrence of complications during catheterization, and improve the quality of life.
2021 Vol. 18 (33): 161-164 [Abstract] ( 114 ) HTML (1 KB)  PDF (659 KB)  ( 29 )
165 Influence of PDCA mode and same level training on nurses’ skill operation level#br#
SHEN Lanfang1 YU Shaoting1 CHEN Linxia2
Objective To explore the influence of PDCA mode and the same level training on nurses’ skills operation level. Methods From January 2017 to December 2018, 40 psychiatric nurses in the Seventh People’s Hospital of Shaoxing, Zhejiang Province (“our hospital” for short) were selected as the control group, and the routine training mode was adopted. A total of 40 psychiatric nurses in our hospital were selected as the observation group from January, 2019 to December, 2020. The qualified rate of skill training, scores of teaching quality, scores of comprehensive nursing ability and nurse satisfaction were compared between the two groups. The training lasted for three months. Results The qualified rate of nurses’ skill training in the observation group was 100% (40/40), which was significantly higher than that in the control group 70% (28/40), and the difference was highly statistically significant (P ＜ 0.01). The score of teaching quality in the observation group were significantly than those in the control group, the differences were statistically significant (P < 0.05). The comprehensive nursing ability score of the two groups was significantly higher than before training, and the observation group was higher than the control group, the differences were statistically significant (P ＜ 0.05); the satisfaction of nurses in the observation group was significantly higher than that in the control group, the difference was statistically significant (P ＜ 0.05). Conclusion PDCA mode and the same level training can improve the skill level of nurses, which is well recognized and can be promoted.
2021 Vol. 18 (33): 165-168 [Abstract] ( 145 ) HTML (1 KB)  PDF (631 KB)  ( 109 )
169 Effect of problem oriented nursing intervention combined with mindfulness decompression training on patients after radical gastrectomy#br#
LI Qian1 SUN Lianhong2 SHA Jiamei3▲
Objective To analyze the nursing effect of problem-based nursing intervention combined with mindfulness decompression training for patients with gastric cancer after radical gastrectomy. Methods The medical records of 100 patients receiving conventional care after radical gastrectomy in Qingdao Municipal Hospital of Shandong Province from January 2017 to June 2018 were retrospectively analyzed as the control group. During the same period, medical records of 100 patients after radical gastrectomy who received conventional nursing + problem-oriented nursing intervention combined with mindfulness decompression training from July 2018 to January 2020 were included as observation group. The stress perception scale (CPSS) and mindful attention awareness scale (MAAS) were used to assess the levels of stress and mindfulness before and at six weeks of intervention. At each time point, self-rating anxiety scale (SAS) and Hamilton depression scale (HAMD) were used to evaluate the degree of anxiety and depression. At six weeks of intervention, nursing satisfaction was recorded and compared between the two groups. Results After six weeks of intervention, the CPSS score of both groups was decreased than that before intervention, and the MAAS score was increased than that before intervention, and the CPSS score of the observation group was lower than that of the control group, and the MAAS score was higher than that of the control group, with statistical significant differences (P ＜ 0.05); after six weeks of intervention, the SAS and HAMD scores of both groups were decreased than those before intervention, and the observation group was lower than the control group, with statistical significant differences (P ＜ 0.05); the total nursing satisfaction rate of the observation group was higher than that of the control group, with a statistical significant difference (P ＜ 0.05). Conclusion Problem oriented nursing intervention combined with mindfulness decompression training can improve the mindfulness level of patients after radical gastrectomy, improve the psychological state, reduce the pressure of patients, and the nursing satisfaction of patients is high.
2021 Vol. 18 (33): 169-172 [Abstract] ( 107 ) HTML (1 KB)  PDF (661 KB)  ( 23 )
173 Application of intervention mode based on IMB model in nursing of patients with postoperative chemotherapy for gastric cancer#br#
ZOU Mengmeng XU Wei▲
Objective To analyze the application effect of the intervention model based on information motivation behavioral skills model (IMB) in nursing of patients with postoperative chemotherapy for gastric cancer. Methods A total of 60 patients with postoperative chemotherapy for gastric cancer in the Department of General Surgery, Nanjing First Hospital (“our hospital” for short) from August 2020 to January 2021 were prospectively selected as subjects. They were divided into observation group and control group by random number table method, with 30 cases in each group. The observation group was given IMB model intervention combined with routine nursing, while the control group was given routine nursing. Before nursing and two months after nursing, self-care ability (assessed by the exercise of self care ability [ESCA] and self-efficacy assessed by general self-efficacy scale [GSES]) of the two groups were evaluated and compared. The nursing satisfaction of the two groups at two months was statistically compared (evaluated by the self-made nursing satisfaction questionnaire of our hospital). Results After two months of nursing, the scores of ESCA dimensions and GSES in both groups increased, and the observation group was higher than those in the control group (P ＜ 0.05). There was no significant difference in total nursing satisfaction between the two groups (P ＞ 0.05). Conclusion The application of intervention mode based on IMB model in nursing of patients with postoperative chemotherapy for gastric cancer can improve the self-care ability and self-efficacy sense of patients, and improve nursing satisfaction.
2021 Vol. 18 (33): 173-176,188 [Abstract] ( 182 ) HTML (1 KB)  PDF (988 KB)  ( 10 )
177 Research on application of cluster nursing based on pain assessment and management in rehabilitation of fracture patients#br#
LIU Keqiong
Objective To explore the application effect of cluster nursing based on pain assessment management in the rehabilitation of fracture patients. Methods A total of 88 patients with fracture treated in Hefei First People’s Hospital from July 2017 to December 2019 were selected and they were divided into observation group and control group by random number table method, with 44 cases in each group. The control group received routine nursing, and the observation group received cluster nursing based on pain assessment management. The compliance of functional exercise was compared between the two groups after seven days of intervention. Visual analog scale (VAS), self-rating depression scale (SDS), and self-rating anxiety scale (SAS) were used to evaluate the scores of pain and psychological status of patients before and after seven days of intervention, and the nursing satisfaction of the two groups after intervention for sevendays was compared. Results After seven days of intervention, the compliance rate of the observation group was higher than that of the control group, the difference was statistically significant (P < 0.05). After seven days of intervention, pain scores in both groups were lower than before, and the observation group was lower than the control group, the differences were statistically significant (P ＜ 0.05). After seven days of intervention, psychological status scores of both groups were lower than before, and the observation group was lower than the control group, the differences were statistically significant (P ＜ 0.05). After seven days of intervention, the nursing satisfaction score of the observation group was higher than that of the control group, the differences were statistically significant (P ＜ 0.05). Conclusion Cluster nursing based on pain assessment management can relieve postoperative pain of fracture patients, improve the mental state of the patient, promote rapid recovery of patients, and increase satisfaction, which  is worth promoting.
2021 Vol. 18 (33): 177-180 [Abstract] ( 99 ) HTML (1 KB)  PDF (626 KB)  ( 166 )
181 Primary small cell carcinoma of breast with three cases literature review#br#
BI Renjie1 HUANG Di1 WANG Likun2 PENG Tao3 LI Shuguang1
Primary small cell carcinoma of the breast (SmCC) is an extremely rare malignancy of the breast. It is characterized by difficult diagnosis, high malignancy and poor prognosis. In clinical practice, SmCC is usually treated with reference to small cell carcinoma of the lung, but SmCC itself has special biological characteristics and clinical characteristics. In this paper, the case data of three cases of breast small cell malignant tumor admitted to the First Affiliated Hospital of Hebei North University from January 2010 to January 2020 were reviewed. Literature reports related to SmCC in recent 20 years at home and abroad were retrieved. In order to improve the understanding of this disease, literature review was carried out on the diagnosis, pathological features and treatment plan of this disease.
2021 Vol. 18 (33): 181-183,196 [Abstract] ( 125 ) HTML (1 KB)  PDF (755 KB)  ( 59 )
184 Bibliometric and visualization analysis of Chinese health expenditure research#br#
LI Yanna WANG Yiqiao CHE Yushi
Objective To analyze the research status and development trends of health expenditure in China, including research cooperation, research hotspots, and research frontiers. Methods Based on the relevant literatures in CNKI of health expenditures from 1992 to 2020, Gephi software was used to visualize the networks of scientific research cooperation and keywords co-occurrence, and CiteSpace was used to perform the burst terms detection. Results The health expenditure of China has undergone two stages of development from 1992 to 2006 and from 2007 to 2020, and had formed a number of institutions and scholars with important scientific influence. Total health expenditure, health expenditure, health expenditure accounting, influencing factors, health expenditure, and health resource allocation were the research hotspots of continuous attention. Analysis of influencing factors, forecast of growth trend, and analysis of recurrent health costs had become the research frontiers in this field. Conclusion The authors of health expenditure research in China have obtained abundant research results, but the future research should strengthen the horizontal cooperation between different institutions, deepen the research on determinants of health expenditure, forecast of health expenditure growth trend, and analysis of recurrent health expenditure.
2021 Vol. 18 (33): 184-188 [Abstract] ( 80 ) HTML (1 KB)  PDF (1361 KB)  ( 117 )
189 Analysis of the ways to enhance the confidence of traditional Chinese medicine culture in the new era#br#
YU Haoran HONG Shuo WU Dongxia
Confidence in traditional Chinese medicine culture means a high degree of recognition of traditional Chinese medicine culture, confidence in the development of traditional Chinese medicine and the universal pursuit of traditional Chinese medicine cultural value. It is the common aspiration of the people and the trend of the times. Confidence in traditional Chinese medicine culture is the premise and basis for inheriting and carrying forward traditional Chinese medicine culture, and the key to the great rejuvenation of Chinese civilization. It has a profound impact on all traditional Chinese medicine people and the Chinese nation. In the new era, it is necessary to enhance traditional Chinese medicine people’s sense of identity by strengthening their consciousness of traditional Chinese medicine culture; to realize the creative transformation and development of traditional Chinese medicine culture by tracing its origin; to enhance the “soft power” and “hard kung fu” of traditional Chinese medicine culture through exchange and reference, so as to lead the great rejuvenation of the Chinese nation from the cultural field.
2021 Vol. 18 (33): 189-192 [Abstract] ( 157 ) HTML (1 KB)  PDF (559 KB)  ( 101 )
193 Comparison and reference of medical science and technology budget management in typical developed countries#br#
LI Mingsui WANG Zhuoran YANG Juntao
Typical developed countries have established national authoritative institutes of medical sciences such as National Institutes of Health, the British Medical Research Council and the Institut national de la Santé et de la Recherche Médicale, etc., to adopt advanced budget management mode and to set up special fund for medical sciences and technology, etc., to provide financial support on medical sciences, and to promote the establishment of the national medical and scientific innovation system. By comparing and learning from the beneficial practices and good experiences of typical developed countries, it is helpful to clarify the operation path and key nodes of medical science and technology innovation funding system in China. That is, the establishment of a new National Academy of Medical Sciences, the establishment of a medical health science fund, and the use of public budget management tools to coordinate medical science and technology innovation resources.
2021 Vol. 18 (33): 193-196 [Abstract] ( 158 ) HTML (1 KB)  PDF (519 KB)  ( 52 )
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