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| Effects of low concentration bisphenol A on calcium spark of female adult rat cardiac myocytes and its mechanism#br# |
| YAN Sujuan1 CHENG Xiaoshu2 LI Juxiang2 CHEN Yamei3 DONG Min3 WANG Hongsheng3 |
1.Department of Cardiology, Beijing Electric Power Hospital, Beijing 100073, China;
2.Department of Cardiovascular Medicine, the Second Affiliated Hospital of Nanchang University, Jiangxi Province, Nanchang 330006, China;
3.College of Medicine, University of Cincinnati, State of Ohio, Cincinnati 45267, the United States of America |
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Abstract Objective To investigate the effects and the mechanisms of bisphenol A (BPA) on calcium spark of the female adult rat cardiac myocytes. Methods Twenty healthy female SD rats weighing 200-250 g at 10-16 weeks old were selected and their cardiac myocytes were isolated in vitro after death. According to the experimental requirements, the cells were divided into control group and BPA group (10-9 mmol/L BPA was added). Cells in each group were obtained from at least three rats, and eight to ten cells were taken from each rat. Confocal microscope was used to detect calcium sparks in both groups, and calcium ion imaging system was used to detect calcium transients and calcium load of sarcoplasmic reticulum in two groups. Results Under confocal microscope, bright calcium sparks appeared in both groups. Calcium spark frequency and calcium transient peak value in BPA group were higher than those in control group, while calcium transient tau value was shorter than that in control group, the differences were highly statistically significant (P ＜ 0.01). There was no significant difference between the two groups in calcium spark peak value (P ＞ 0.05). In steady state, the calcium load of sarcoplasmic reticulum in BPA group was higher than that in control group, and the difference was statistically significant (P ＜ 0.05). There was no significant difference in tau value of sarcoplasmic reticulum calcium load between the two groups (P ＞ 0.05). At static state, there was no significant difference in the calcium load of sarcoplasmic reticulum between the two groups (P ＞ 0.05). Conclusion Under the action of BPA, the calcium load in the sarcoplasmic reticulum of female adult rat cardiomyocytes gradually increased with the contraction of cardiomyocytes, and the opening rate of renotin receptor also gradually increased, promoting Ca2+ leakage from the sarcoplasmic reticulum of cardiomyocytes during diastolic period, and increasing the frequency of calcium spark.
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