中国医药导报
      Home                                                                                    中文
中国医药导报
 
Forthcoming Articles
Current Issue
Archive
Advanced Search
Read Articles
Download Articles
Email Alert
 
Quick Search  
  Adv Search
2018 Vol. 15, No. 31
Published: 2018-11-05
 
 
4 Effect of miRNA-1 and miRNA-133 overexpression on myocardial differentiation of inducible pluripotent stem cells
WANG Fangfang BAI Baobao▲ ZENG Di CHU Yi LI Xue
Objective To investigate the effect of miRNA-1 and miRNA-133 overexpression on myocardial differentiation of inducible pluripotent stem cells (iPSC). Methods Embryos from two-week-old American Institute of Cancer Research (ICR) pregnant mice were collected, 0.5 g/L of trypsin was used for digestion of vaccination, mitomycin was used after spreading to three generations, mouse induced pluripotent stem cells (miPSC) was incubated and cultivated at 37℃, miPSC growed on embryonic fibroblasts was digested and passaged. Medium was replaced, miRNA (U6) (10 nmol/L, marked as control group), miRNA-1 (10 nmol/L, marked as miRNA-1 group), miRNA-133 (10 nmol/L, marked as miRNA-133 group), miRNA-1 and miRNA-133 (10 nmol/L respectively, marked as miRNA-1+133 group) were added and transfected for 24 hours, and were washed by PBS, then miPSC differentiation medium was added, and its differentiation was daily observed, cell pulse rate was calculated. RT-PCR was used to detect the expression of miRNA-1, miRNA-133 and cardiac troponin T (cTnT). Results The cTnT was barely expressed in undifferentiated miPSC, compared with 0 day  of differentiation, the expreesion level of cTnT of miRNA-1 group and miRNA-133 group was increased after 5 days of differentiation (P ＜ 0.05), the increase was more obvious after 10 days of differentiation (P ＜ 0.01). After 10 days of differentiation, the expression level of cTnT of miRNA-1+133 group was obviously higher than those of miRNA-1 group and miRNA-133 group (P ＜ 0.01). Compared with control group, the cell pulse rate of miRNA-1 group and miRNA-133 group were not increased (P ＞ 0.05), while the cell pulse rate of miRNA-1+133 group was significantly increased (P ＜ 0.01). Conclusion miRNA-1 or miRNA-133 alone does not promote myocardial differentiation, only after the co-expression of miRNA-1 and miRNA-133, it can significantly improve the directional differentiation efficiency of miPSC to cardiac cells.
2018 Vol. 15 (31): 4-7,15 [Abstract] ( 290 ) HTML (1 KB)  PDF (804 KB)  ( 110 )
8 Effect of  IGF-1 on learning and memory ability in rats with global cerebral ischemia
LI Yongli1 ZHAO Jing2 LIU Liming1 ZHANG Quanbo1 LIU Li1 WU Bihua1
Objective To investigate the effect of exogenous insulin-like growth factor-1 (IGF-1) on learning and memory ability in rats with global cerebral ischemia and its possible mechanism. Methods A total of 110 Wistar male rats were divided into normal group, sham operation group, global cerebral ischemia model group, model administration group 1 and model administration group 2; there were 10 rats in each of the normal group and sham operation group. There were 30 rats in each of the whole cerebral ischemia model group, the model drug administration group 1 and the model drug administration group 2. All operated groups were embedded micro-injection catheter into the lateral ventricle, and the whole cerebral ischemia model was established on the 6th day after catheterization with modified Pusinelli four-vessel blockade. Rats in the global cerebral ischemia model group, model drug administration group 1, and model drug administration group 2 were injected with 10 μL of normal saline, IGF-1 (0.2 μg/μL) 10 μL, and IGF-1+PPP (after intraperitoneal injection of blocker PPP 20 mg/kg for 30 min, IGF-1 (0.2 μg/μL) 10 μL was injected into the lateral ventricle), once a day for 7 d. In the sham operation group, NS 10 μL was injected into the lateral ventricle of rats. Morris water maze test was used to test the learning and memory ability of rats in each group before and after administration. HE staining was used to observe the apoptosis of hippocampal CA1 neurons. Immunohistochemistry was used to detect the expression of p-Akt and p-mTOR proteins in hippocampus. Results Compared with the global cerebral ischemia model group, the average escape latency of the model administration group 1 was significantly shortened (P < 0.05), the number of straddle platform and the original platform quadrant swimming time increased significantly (P < 0.05); the number of cells in the hippocampal CA1 area increased. The arrangement was uniform and the structure was clear; the expression of p-Akt and p-mTOR protein were higher (P < 0.05). Conclusion The learning and memory ability in rats with global cerebral ischemia was improved by exogenous IGF-1. This may be related to the inhibition of apoptosis in the hippocampal CA1 region and the enhancement of the expression of p-Akt and p-mTOR proteins in the PI3K/Akt signaling pathway.
2018 Vol. 15 (31): 8-11 [Abstract] ( 200 ) HTML (1 KB)  PDF (672 KB)  ( 115 )
12 Effect of Qizi Yishen Lichong Decoction on inflammatory cytokines and insulin resistance of ovarian epithelial cells in PCOS insulin-resistant rats
CHEN Haiyan TANG Ling▲ XIAO Chengcong
Objective To analyze the effect of Qizi Yishen Lichong Decoction on inflammatory cytokines and insulin resistance in ovarian epithelial cells of rats with polycystic ovary syndrome (PCOS) insulin resistance. Methods A total of 60 SPF Wistar male rats were selected to prepare insulin-resistant PCOS model rats. A total of 54 rats were enrolled in this study. Random number table method was used to divide them into observation group (27 rats) and control group (27 rats). The observation group was given  intragastric administration of 6 mL/kg of Qizi Yishen Lichong Decoction, the control group was given intragastric administration of distilled water of equal dose, both groups were given gastric lavage once a day for 20 days. The level of serum follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (T) were detected by chemical immunofluorescence method. The content of inflammatory cytokine pigment epithelium derived factor (PEDF), Visfatin and interleukin-6 (IL-6) in ovarian epithelial cells were detected by enzyme-linked immunosorbent assay (ELISA). The levels of serum fasting insulin (FINS) and fasting blood glucose (FBG) were measured by radioimmunoassay, gastric tube glucose after 2 hours of blood glucose (OGTT-2 h BG) content was detected by glucose oxidase method, insulin resistance index (HOMA-IR) and insulin sensitivity index (ISI) were calculated. Results The levels of LH, T, LH/FSH of the observation group were lower than those of the control group (P ＜ 0.05). The contents of serum FBG, OGTT-2 h BG, FINS and HOMA-IR of the observation group were significantly lower than those of the control group, while ISI was higher than that of the control group, the differences were statistically significant (P ＜ 0.05). The contents of PEDF, Visfatin and IL-6 of the epithelial cells of the observation group were lower than those of the control group, and the differences were statistically significant (P ＜ 0.05). Conclusion Qizi Yishen Lichong Decoction can reduce the content of inflammatory cytokines in ovarian epithelial cells of PCOS insulin-resistant rats, regulate the ratio of LH/FSH, improve hyperinsulinemia and hyperandrogenism, and adjust the disorder of glucose metabolism.
2018 Vol. 15 (31): 12-15 [Abstract] ( 301 ) HTML (1 KB)  PDF (612 KB)  ( 136 )
16 Study on the inhibition effect of Tiaojing Zanyu Pills for oxytocin induced contraction of uterine smooth muscle in vitro in rats
WANG Jingjing1 WANG Zhang2
Objective To observe the effect of Tiaojing Zanyu Pills on isolated uterine smooth muscle in rats. Methods A certain dose of Estradiol Benzoate was administered intraperitoneally to induce allergy in rats. After 24 h, the rats were killed and the smooth muscle of the uterus was removed, and then oxytocin was added to induce constriction in the smooth muscle of the uterus. Finally, suspension with three different concentrations (0.1854, 0.3705, 0.7394 mg/mL) of Tiaojing Zanyu Pills were added respectively. Results Tiaojing Zanyu Pills had no obvious effects on normal rats in terms of the average tension, frequency and activity of uterine smooth muscle contraction (P ＞ 0.05). However, the medium concentration (0.3705 mg/mL) and high concentration (0.7394 mg/mL) of Tiaojing Zanyu Pills could obviously inhibit the oxytocin which thereby induced contraction of uterine smooth muscle in rats, thus resulting in decrease in the average tension and activity (P ＜ 0.05), but no obvious effect on the contraction frequency was observed (P ＞ 0.05). Conclusion Tiaojing Zanyu Pills can directly inhibit the pathological conditions of the uterine smooth muscle contraction, which may be one of the mechanism of clinical treatment of dysmenorrhea.
2018 Vol. 15 (31): 16-19 [Abstract] ( 303 ) HTML (1 KB)  PDF (609 KB)  ( 110 )
20 Establishment of decoction kinetic model of Astragalus membranaceus of Buyang Huanwu Decoction
LIANG Shunguang1,2 WANG Shengxin1 WANG Lisheng1
Objective To determine the content of calycosin-7-glucoside in Buyang Huanwu Decoction, and to establish the decoction kinetic model of Astragalus membranaceus of Buyang Huanwu Decoction. Methods The content of calycosin-7-glucoside in Buyang Huanwu Decoction was determined by high performance liquid chromatography. The chromatographic column was Agilent C18 column (250 mm×4.6 mm, 5 μm), mobile phase was acetonitrile (A)-0.05% aqueous solution of phosphoric acid (B) with gradient elution, flow rate was 1 mL/min and detection wavelength was 230 nm, the sample volume was 10 μL. Based on the Fick′s diffusion law, the equation parameters were deduced and fitted. Results Calycosin-7-glucoside was in a good linear relationship in the range of 0.10-0.60 μg, the regression equation was y = 35.95x - 0.3605, R2 = 0.9999, the kinetic equation of decoction was C■=■t■■, the verification result was good. Conclusion The kinetic model established in this study is in good agreement with the experimental results. The standard deviation (0.3013%) of the experimental values and the calculated values under each condition can basically meet the requirements of industrial standard deviation.
2018 Vol. 15 (31): 20-24,29 [Abstract] ( 260 ) HTML (1 KB)  PDF (1002 KB)  ( 126 )
25 Optimization of extraction process of total terpenoids from Tripterygium wilfordii suspension cells based on orthognal design and multi-index comprehensive evaluation method
WU Xiaoyi ZHANG Keyi ZHANG Rui WANG Changhai
Objective To optimize the extraction process of total terpenoids from Tripterygium wilfordii suspension cells. Methods The contents of triptolide, triptophenolide and celastrol were determinated by UPLC-QQQ-MS. The separation and analysis were performed on Waters HSS T3 chromatographic column (2.1 mm ×100 mm, 1.8 μm), with 0.1% acetic acid solution-acetonitrile as the mobile phase for gradient elution. The column temperature was set at 30℃ with flow rate of 0.3 mL/min, and the sample volume was 5 μL. The composite score of 3 kinds of terpenes contents was used as index, based on single factor tests, three parameters (methanol concentration, extraction time and solvent volume) were evaluated with an L9 (34) orthogonal design. The feasibility was checked by validation tests. Results The optimum extracting conditions of total terpenoids from Tripterygium wilfordii suspension cells were 50 times volumes of 80% methanol as the solvent and ultrasonic extracting 0.5 h after dipping 12 h. In this condition, the average yields of triptolide, triptophenolide and celastrol were (52.36±0.60), (170.73±1.57), (388.14±5.89) μg/g, respectively. The comprehensive score was stable. Conclusion The extraction process of total terpenoids from Tripterygium wilfordii suspension cells based on orthognal design and multi-index comprehensive evaluation is reasonable, repeatable and suitable for massive extraction of total terpenoids from Tripterygium wilfordii suspension cells.
2018 Vol. 15 (31): 25-29 [Abstract] ( 235 ) HTML (1 KB)  PDF (741 KB)  ( 64 )
30 Study on preparation of high purity ginkgolide by atmospheric pressure silica gel column chromatography
ZHENG Xiangwei1 WU Peiying2 WANG Jun2 WANG Dandan2 RUAN Kefeng1 LIANG Shuang1 FENG Yi1
Objective To explore an economical and practical scale preparation technique for high purity ginkgolide (≥80%). Methods Based on the content and transfer rate, the key parameters affecting the chromatographic separation were investigated by single factor, and the optimal technological route of separating and purifying ginkgolide from extract of ginkgo biloba leaves (GBE) was screened out. The total lactones of ginkgo was detected by high performance liquid chromatography (Chinese pharmacopoeia 2010 edition 1 appendix Ⅵ D). Chromatographic conditions included octadecyl silane bonded silica gel being filler, methanol-water (21∶79) being mobile phase, detection by differential refractive index detector, column temperature being at 30℃. The number of theoretical plates should be no less than 2500 according to the peak of bilobalide, ginkgolide A, ginkgolide B and ginkgolide C. Results The results showed that the use of silica gel column chromatography combined with recrystallization process achieved the purpose. The optimal process parameters were as follows: silica gel with particle size of 200-300 mesh, packed column by way of wet method (diameter to diameter ratio 1∶10), loaded sample by way of dry method (loading ratio 1∶50), eluted by means of dichloromethane-methanol (40∶1, 35∶1, V/V) successively, the eluted products were dissolved in acetone-water (1∶1, V/V) and allowed to stand overnight and filter crystallization, so as to obtain the total ginkgolides with the purity of ≥80%, in which the total recovery was 55.4%. Conclusion The process requires simple equipments, large processed capacity, and low production costs. It has industrial production potential.
2018 Vol. 15 (31): 30-33 [Abstract] ( 307 ) HTML (1 KB)  PDF (846 KB)  ( 125 )
34 Study on the improving the quality standard of Shuqing Granules
CHE Hongwei YANG Haining WANG Dengcai ZHENG Wensheng
Objective To improve the quality standard of Shuqing Granules. Methods The qualitative identification experiments of Folium Isatidis, Glycyrrhizae Radix et Rhizoma, Mori Folltfm and Phragmitis Rhizoma were carried out by thin-layer chromatography. The content of indirubin and glycyrrhizin were detected by reverse high performance liquid chromatography useing Diamonsil (diamond) C18 (250 mm×4.6 mm, 5 μm) as chromatographic column. The content of indirubin was detected with the mobile phase being methanol-acetonitrile-0.4% phosphoric acid solution (33.5∶33.5∶33), column temperature being at 35℃, detection wavelength being at 238 nm. The content of glycyrrhizin was detected with mobile phase being methanol-glacial acetic acid-0.2 mol/L ammonium acetate solution (67∶1∶33), column temperature being at 35℃, detection wavelength being at 238 nm. Results Thin-layer chromatogram of Folium Isatidis, Glycyrrhizae Radix et Rhizoma, Mori Folltfm and Phragmitis Rhizoma were clear spots with suitable resolution and proper Rf value, no interference with negative control. The regression equation of indirubin and glycyrrhizin were y = 1471.2799x - 52.6798 (r ＝ 0.9992) and y = 356 584x - 2427.7 (r ＝ 0.9995), respectively. The linear range of indirubin and glycyrrhizin ranged from 0.057-0.46 μg/mL and 0.2-1.4 mg/mL respectively, and showed a good linear relationship. The average recovery rate of indirubin and glycyrrhizin were 99.43% and 100.00%, RSD were 0.81% and 0.65% (n = 6). Conclusion All the experimental methods are simple and reproducible, and can be used for the quality control of Shuqing Granules.
2018 Vol. 15 (31): 34-37,52 [Abstract] ( 265 ) HTML (1 KB)  PDF (1175 KB)  ( 86 )
38 Study on quality standard of Weiyanling Granules
QIAN Mengxi1 ZHANG Yongmei1 KANG Zhen1 QIAN Wei1 YU Hui1 LI Jianfeng2▲
Objective To establish the quality standard of Weiyanling Granules. Methods Astragali Radix, Coptidis Rhizoma, Glycyrrhizae Radix et Rhizoma in Weiyanling Granules were identified by thin-layer chromatography (TLC). The contents of geniposide and paeoniflorin in Weiyanling Granules were determinated by high performance liquid chromatography (HPLC) simultaneously. The HPLC method was established with Diamonsil C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was consisted of methyl alcohol-water (20∶80, V/V) with the flow rate of 1 mL/min. The detective wavelength was set at 235 nm, and the column temperature was at 25℃. Results TLC spots were clear and well-separated without interference from negative sample. The linear range of geniposide and paeoniflorin were 1.030-10.300 μg and 1.032-10.320 μg with the average recovery being 98.92% and 97.75%, RSD being 1.39% and 1.19%, respectively. Conclusion The method is simple, feasible, reproducible and accurate, and is suitable for the quality control of Weiyanling Granules.
2018 Vol. 15 (31): 38-42 [Abstract] ( 274 ) HTML (1 KB)  PDF (948 KB)  ( 68 )
43 Determination of the acid amino acid in the Compound Amino Acid Injections with dynamic pH junction-sweeping combined with indirect UV detection method
GUO Hailong XU Xiaoying LI Yuzhen
Objective To detect the content of glutamic acid (Glu) and aspartic acid (Asp) in the Compound Amino Acid Injections. Methods Dynamic pH junction-sweeping in combination with indirect UV detection method was adopted. The running buffer (pH 7.5) consists of 10 mmol/L Tris, 8 mmol/L benzoic acid and 10 mmol/L β-cyclodextrin. The sample matrix was 3 mmol/L H3PO4 solution (pH 2.5). Pressure was adopted for sample injection, and the sample injection pressure and time was 1.0 psi and 53 s, respectively. The applied voltage was 12 kV. Indirect UV detection using benzoic acid as UV probe was adopted. And the detection wavelength was set at 214 nm. Results The Glu and Asp in the Compound Amino Acid Injections were 95.9%-104.7% of their labeled amount, and the results agreed with the content limit range (95.0%-105.0%) in Chinese Pharmacopeia (2015 edition). Conclusion Dynamic pH junction-sweeping combined with indirect UV detection method can be used to determine the content of acid amino acid in Compound Amino Acid Injections. And the contents of Glu and Asp in the Compound Amino Acid Injections are in accordance with the regulations.
2018 Vol. 15 (31): 43-46 [Abstract] ( 223 ) HTML (1 KB)  PDF (641 KB)  ( 119 )
47 Research progress on chemical composition and pharmacological effects of Kalimeris indica (L.) Sch-Bip
ZHANG Mei1 ZHOU Xiaowei2 ZHANG Yuxin3 LI Li1 RAO Gaoxiong1
Kalimeris indica (L.) Sch-Bip is one of the most popular common traditional Chinese plants. It is not only rich in resources, but also diverse in chemical composition. The chemical composition of sterides, terpenoids, flavonoid, phenolic acid, volatile oils for Kalimeris indica (L.) Sch-Bip have been reported according to the recent research. Kalimeris indica (L.) Sch-Bip is also with wide range of pharmacological effects, such as antibacterial, anti-inflammatory, anti-oxidation, and so on, which is determined by its rich chemical composition. This paper summarizes and expounds the research status chemical composition and pharmacological action of Kalimeris indica (L.) Sch-Bip at home and abroad by referring to a large number of  literatures, in order to provide references for the comprehensive understanding and rational development of Kalimeris indica (L.) Sch-Bip resources.
2018 Vol. 15 (31): 47-52 [Abstract] ( 353 ) HTML (1 KB)  PDF (614 KB)  ( 403 )
53 The research progress of lncRNA and cardiovascular diseases
ZHANG Haitao LIN Wenyong WANG Xiaolong XIE Manman WANG Yingjie
Cardiovascular disease is the leading cause of death worldwide. The prevalence and mortality of cardiovascular disease in China is still rising year by year. Long-chain non-coding RNAs (lncRNAs) are a new class of non-coding RNAs that are involved in a variety of biological processes. Abnormalities of lncRNAs are directly related to human cardiovascular diseases. Abnormal lncRNA regulation loops are associated with cardiac hypertrophy, vascular disease, atherosclerosis, and dyslipidemia. This article reviews recent advances in long-chain non-coding RNA and cardiovascular disease.
2018 Vol. 15 (31): 53-55,59 [Abstract] ( 448 ) HTML (1 KB)  PDF (600 KB)  ( 162 )
56 Risk factors and interventions for maternal anxiety and depression
XIA Huanhuan HE Yanling
Pregnancy and childbirth are very important physiological stage in a women′s life. Pregnant women have a corresponding psychological stress response during this period. Among them, anxiety and depression are the most common. This is closely related to the physical, social and psychological factors of women during pregnancy and childbirth. Experience anxiety and depression for a long-term can harm maternal physical and mental health, and affect family life, and can lead to increased risk of cesarean section, low birth weight, postpartum lactation, postpartum hemorrhage, and postpartum depression. At present, prenatal health education, psychosomatic intervention and other measures are often used to alleviate maternal anxiety and depression. This article reviews the factors affecting maternal anxiety and depression and intervention methods to provide a basis for clinical treatment of maternal anxiety and depression.
2018 Vol. 15 (31): 56-59 [Abstract] ( 244 ) HTML (1 KB)  PDF (586 KB)  ( 106 )
60 Advances in the interaction of Dax1 with embryonic stem cells multipotential network factors Oct4, Sox2, Nanog, and Esrrb
SUN Huilin YANG Dan HU Zhe▲
The self-renewal and pluripotency of embryonic stem cells (ESCs) are maintained by the large and complex interconnected transcriptional regulatory network formed by transcription factors such as Oct4, Sox2, Nanog, and Esrrb, and Dax1 can also participate in the regulation of ESCs. Dax1 interacts with core factors such as Oct4, Sox2, Nanog and Esrrb, and participates in maintaining characteristics of ESCs and inhibiting ESCs differentiation. Dax1 can also produce a similar promotion to Nanog in the later stages of induction of induced pluripotent stem cells (IPSCs). To study the role, function, status and interaction of Dax1 in the ESCS transcriptional regulatory network and the induction of IPSCs, and the specific mechanisms of interaction with other transcription factors can be helpful for stem cell mechanism research and clinical therapeutic transformation research. At present, there are few studies on the role of Dax1 in embryonic stem cells. This paper reviews the existing research results in order to provide reference for further research and clinical treatment transformation of embryonic stem cells.
2018 Vol. 15 (31): 60-63 [Abstract] ( 262 ) HTML (1 KB)  PDF (616 KB)  ( 112 )
64 Research progress of traditional Chinese medicine on anti-Staphylococcus aureus
DU Chunyu WANG Jiahe▲
Staphylococcus aureus (SA) is one of the most common pathogenic bacteria of hospital and community acquisition. In recent years, the trend of outbreak of drug-resistant strains of Staphylococcus aureus is gradually serious, but the effective treatment program is extremely limited, resulting in high morbidity and mortality of its infection. Numerous practices have proved that the antibacterial effect of traditional Chinese medicine is beyond doubt, especially for the resistant strains that are ineffective in antibiotic treatment, traditional Chinese medicine has a broad application prospect. This paper reviews the research progress of Chinese medicine in the treatment of Staphylococcus aureus and provides a new idea for guiding the clinical development of effective treatment.
2018 Vol. 15 (31): 64-67 [Abstract] ( 366 ) HTML (1 KB)  PDF (595 KB)  ( 139 )
68 Application and evaluation of BOPPPS combined with CBL teaching methods in the clinical practice of gynecology and obstetrics
LI Jia ZHANG Wei CHEN Biliang YANG Hong LIU Shujuan
Objective To investigate the application effect of combination of BOPPPS and case-based learning (CBL) teaching methods in clinical practice of obstetrics and gynecology. Methods Two hundred and seventeen grade 2014 five-year undergraduates who attended clinical practice in Department of Gynecology and Obstetrics, Xijing Hospital were selected from May to October in 2017. They were divided into experimental group (108 undergraduates) and control group (109 undergraduates) by random number table method. The experimental group was taught by combination of BOPPPS and CBL teaching method, and the control group was taught by traditional teaching method. Both groups were in clinical practice for two weeks. The teaching effect was evaluated via examination and questionnaire investigation after teaching. Results Undergraduates accepted the teaching method of the combination of BOPPPS and CBL. Case analysis scores, clinical operating scores and the total scores of the experimental group were much higher than those of the control group with significant differences (P ＜ 0.05). The acceptable degree of the experimental group was better than those of control group in fields such as inspiring learning interest, mastery of the key points and difficulties, ability of clinical thought, clinical operation skill, summary and literature review ability, rationality of teaching organization, interaction between teachers and students, and the differences were statistically significant (P ＜ 0.05). Conclusion Combination of BOPPPS and CBL teaching methods can improve learning efficiency, interest and activity, and enhance the clinical thought, improve clinical skill, which contributes to satisfied teaching results and is worth promotion.
2018 Vol. 15 (31): 68-71 [Abstract] ( 243 ) HTML (1 KB)  PDF (485 KB)  ( 193 )
72 Research on value and current situation of literature education in the universities of Chinese medicine
ZHANG Lingli
Literature education has considerable important position in higher Chinese medical education. On the basis of special properties of Chinese medicine, the paper aims at probing into relationship between Chinese medical education and literature education; analyzing present situation of literature education in Chinese medicine universities; in consideration of mingled hope and fear situation of literature education in Chinese medicine universities, focusing on what and how the activity should be taken by literature education teachers. The priorities of this article is not only in view of the efficacy from literature education on the Chinese medicine education, but also on the analysis via differentiation on the two aspects of teaching object and courses in the Chinese medicine education. Meanwhile, this article concentrates on the concrete practitioner-teachers, and further studies the better methods to achieve the great value of literature education.
2018 Vol. 15 (31): 72-75 [Abstract] ( 251 ) HTML (1 KB)  PDF (455 KB)  ( 74 )
76 Relationships of MYC/Bcl-2 co-expression with clinical features and prognosis of diffuse large B-cell lymphoma
HUANG Ya′ni GE Wei▲
Objective To analyze the relationships of MYC/Bcl-2 proteins co-expression of clinical features and prognosis of diffuse large B-cell lymphoma (DLBCL), in order to explore the significance with MYC/Bcl-2 protein co-expression in DLBCL． Methods From January 2012 to January 2014, in Renmin Hospital of Wuhan University, clinical data of 59 DLBCL patients were collected, and the expression of MYC and Bcl-2 protein was analyzed by immunohistochemistry for their pathological tissue samples,  the relationships of MYC/Bcl-2 co-expression with clinical features and prognosis were analyzed. Results Among 59 patients, there were 19 MYC/Bcl-2 co-expression patients, accounting for 32.2%． MYC/Bcl-2 co-expression was more easily detected in patients with later stage and higher IPI scores, the differences were statistically significant (P < 0.05). The 5-year OS of MYC/Bcl-2 co-expression patients was 26.3%, it was significantly lower than that of non-co-expressed patients (77.5%), the difference was statistically significant (P < 0.01), the Cox proportional hazards model showed MYC/Bcl-2 co-expression was associated with prognosis (OR = 0.170, 95%CI: 0.077-0.376, P < 0.01). Conclusion MYC/Bcl-2 co-expression is associated with the prognosis of diffuse large B-cell lymphoma.
2018 Vol. 15 (31): 76-79 [Abstract] ( 268 ) HTML (1 KB)  PDF (550 KB)  ( 92 )
80 Comparison of efficacy of extracorporeal shockwave and autologous perididymal patch grafting for Peyronie′s disease
ZHU Guangyuan1* ZHANG Zhiguo2* ZHANG Wenda2 HAN Conghui1，2▲
Objective To compare and analyze the clinical efficacy of extracorporeal shockwave and autologous perididymal patch grafting for Peyronie′s disease. Methods From June 2010 to January 2016, in Xuzhou Central Hospital, l20 patients with  Peyronie′s disease  were selected, according to the random number method, they were divided into control group and observation group, with 10 cases in each group. The control group was treated with extracorporeal shock wave, and the observation group was treated with autologous perididymal patch grafting. The international index of erectile function-5 (IIEF-5), penile curvature between the two groups before and after treatment were compared and analyzed. Results The IIEF-5 score, induration size and penile curvature before and after treatment in control group were compared, the differences were not statistically significant (P > 0.05). After treatment, in the observation group, the IIEF-5 score was significantly higher than that before the treatment, the difference was statistically significant (P < 0.05); penile induration was no recurrence after treatment, the size of penile induration before and after treatment was compared, and the difference was statistically significant (P < 0.05); the penile curvature after treatment was lower than that before treatment, the difference was statistically significant (P < 0.05). The IIEF-5 score, induration size and penile curvature in the two groups after treatment were compared, the differences were statistically significant (P < 0.05). Conclusion The efficacy of extracorporeal shock wave therapy for penile induration is still doubtful. Autologous perididymal patch grafting is a safe and effective surgical method for the treatment of Peyronie′s disease.
2018 Vol. 15 (31): 80-82,110 [Abstract] ( 289 ) HTML (1 KB)  PDF (912 KB)  ( 133 )
83 Effect of ultrasound-guided ozone injection combined with polarized light irradiation in treatment of plantar fasciitis
GUO Yu′na1 YAN Wei2 ZHANG Jie3 DOU Zhi1 LI Xiaolin1 LIU Jingjie1 YANG Liqiang1▲ NI Jiaxiang1
Objective To observe the clinical effect of ultrasound-guided ozone injection combined with polarized light irradiation in treatment of plantar fasciitis. Methods A total of 90 patients with plantar fasciitis treated in Department of Pain Management, Xuanwu Hospital, Capital Medical University from October 2016 to July 2017 were collected and divided into three groups by random number table method, with 30 cases in each group. Group A was treated with injection therapy of plantar pain point, Group B was treated with the pain point injection + infrared polarized light irradiation, Group C was treated by ultrasound-guided pain point injection + ozone injection+ infrared polarized light irradiation. The pain relief degree and quality of life (QOL) score were compared among the three groups before and after treatment for 1 week, 3 months, 6 months. Results The pain of the three groups was significantly relieved after treatment for 1 week, 3 months, 6 months. After treatment for 1 week, amount of cases with superior effect in Group B and C were larger than that in Group A (P ＜ 0.05). After treatment for 3 months, amount of cure cases in Group C was larger than that in Group A and B, and amount of cases with superior effect in Group B and C were larger than that in Group A (P ＜ 0.05). After treatment for 6 months, amount of cure cases in Group C was larger than that in Group A and B, and amount of cases with superior effect in Group C was larger than that in Group A (P ＜ 0.05). The QOL scores in the three groups after treatment for 1 week, 3 months, 6 months were higher than those before treatment (P ＜ 0.01), and after treatment for 6 months, the QOL score in Group C was higher than that in Group A (P ＜ 0.05). Conclusion Ultrasound-guided ozone injection combined with polarized light irradiation is a safe and effective therapy in treating plantar fasciitis, that is worth of clinical promotion.
2018 Vol. 15 (31): 83-86 [Abstract] ( 280 ) HTML (1 KB)  PDF (663 KB)  ( 147 )
87 The relationship between constitution type of traditional Chinese medicine and chronic diseases in the elderly with lower weight
SHI Mai1 SHI Xuefeng2 WANG Dan2 CHEN Yu1 BAI Qian2 WANG Xi2 GUO Yiwei1 JIN Jiangli3
Objective To investigate the relationship among the traditional Chinese medicine constitution type, chronic disease and skeletal muscle quality and strength of elderly people with low weight (body mass index < 18.5 kg/m2). Methods A total of 229 elderly with low weight who had first visit to outpatient of China-Japan Friendship Hospital from October to December 2014 were enrolled in this study. Constitution type of traditional Chinese medicine scale combined with four diagnosis were used to confirm the constitution type, related medical records were looked up to confirm the diagnosis, and body composition analyzer, grip test were used to judge the sarcopenia. Results The sequence of traditional Chinese medicine constitution type of low weight elderly were Qi-depression, Yang-deficiency, Qi-deficiency, blood stasis, gentleness, Wet-heat, special diathesis, Yin-deficiency type. There were 178 elderly people suffered from chronic disease (77.73%), and there were relationships between chronic disease and the distribution of traditional Chinese medicine constitution type (P ＜ 0.001). There were 168 elderly people were diagnosed with sarcopenia (73.36%), and there was relationship between sarcopenia and the distribution of traditional Chinese medicine (P ＜ 0.001). There were 161 elderly people were diagnosed with chronic disease and sarcopenia (70.31%), and there was correlation between chronic disease and sarcopenia in low weight elderly people (P ＜ 0.001). Conclusion Physical characteristics of traditional Chinese medicine should be taken into account of prevention and treatment of chronic diseases in low weight elderly people and the muscle quality and function should be concerned with.
2018 Vol. 15 (31): 87-89 [Abstract] ( 278 ) HTML (1 KB)  PDF (509 KB)  ( 123 )
90 Effect of laparoscopic appendectomy on oxidative stress and inflammatory response in patients with acute perforated appendicitis
ZHANG Ying CAO Yu LI Zhongmin
Objective To investigate the effect of laparoscopic appendectomy on oxidative stress and inflammatory response in patients with acute perforated appendicitis. Methods A total of 97 patients with acute perforated appendicitis admitted to Liaoyang Central Hospital from February 2013 to March 2018 were selected. According to random number table method, the patients were divided into control group (open appendectomy, 48 cases) and study group (laparoscopic appendectomy, 49 cases). Clinical indices in the two groups after operation were compared, as well as levels of oxidative stress index [superoxide dismutase (SOD), malondialdehyde (MDA)], levels of inflammatory cytokines [interleukin-6 (IL-6), C reactive protein (CRP), tumor necrosis factor-α (TNF-α)] before and 1 day after operation. At the same time, the complications of the two groups were observed. Results The amount of bleeding, the time of operation, the time of anus exhaust, the time of body temperature recovery and the time of hospitalization in the study group were all lower than those in the control group, the differences were statistically significant between the two groups (P ＜ 0.05). One day after operation, SOD in the two groups were lower than those before operation, and SOD in the control group was lower than that in the study group (P ＜ 0.05). One day after operation, MDA in the two groups were higher than those before operation, and MDA in the control group was higher than that in the study group (P ＜ 0.05). The levels of IL-6, CRP and TNF-α in the two groups were higher at 1 d after operation than those before operation, but these indices in the study group were lower than those in the control group (P ＜ 0.05). The incidence of complications after operation in the study group was 10.20%, which was significantly lower than that in the control group (35.42%), and the difference was statistically significant (P ＜ 0.05). Conclusion Laparoscopic appendectomy for acute perforated appendicitis can significantly improve the clinical indices, decrease the oxidative stress and inflammatory response, and reduce the incidence of complications after operation.
2018 Vol. 15 (31): 90-93 [Abstract] ( 228 ) HTML (1 KB)  PDF (555 KB)  ( 115 )
94 Effects of different doses of Dexmedetomidine on Morphine consumption in patients with frequent angina before coronary artery bypass grafting
WANG Yuanyuan1 HU Haijuan2 LIU Lei1 LI Bin1 LI Zhaobin1
Objective To investigate the effect of different doses of Dexmedetomidine (Dex) on Morphine consumption in patients with frequent angina before coronary artery bypass grafting. Methods A total of 100 patients undergoing coronary artery bypass grafting in the Third Hospital of Hebei Medical University from January 2016 to January 2018 were selected. They were divided into group C, Dex 1 group, Dex 2 group, Dex 3 group, by random number table method, 25 cases in each group. The group C was given the 0.1 mg/kg load of Morphine intravenously at the time of angina pectoris; the Dex group was treated with different doses of Dex [Dex 1 group with 0.2 μg/(kg·h), Dex 2 group with 0.5 μg/(kg·h), Dex 3 group with 0.7 μg/(kg·h)], and on the basis of the patients′ angina pectoris condition, the amount of 0.1 mg/kg load was given. Intravenous injection of morphine. Before (T0) and 1 h (T1), 3 h (T2), 6 h (T3), 12 h (T4), 24 h (T5) after the heart rate (HR), mean arterial pressure (MAP), pulse oxygen saturation (SpO2), total frequency of angina pectoris, Morphine dosage and Ramsay score were recorded. Results The HR at T2-T5, Morphine dosage, and frequency of angina pectoris of Dex group were lower than group C (P < 0.05). From T0 to T5, MAP, RR, SpO2 in each groups had no statistically significant difference (P > 0.05), HR at T2-T5, Morphine dosage, frequency of angina pectoris of Dex 3 group were lower than Dex 1 group (P < 0.05), but, there was no difference between Dex 1 group and Dex 2 group (P > 0.05). From T2 to T5, Ramsay score of Dex 3 group was higher than Dex 1 group and Dex 2 group significantly (P < 0.05). Conclusion Continuous administration of a small dose of Dex [0.2 μg/(kg·h)] to patierts with frequent angia before coronary artery bypass grafting can effectinely control the onset of angina pectories.
2018 Vol. 15 (31): 94-98 [Abstract] ( 242 ) HTML (1 KB)  PDF (567 KB)  ( 161 )
99 Effect of continuous pump injection of Methoxamine Hydrochloride Injection assisted intravenous-inhalation combined general anesthesia on intraoperative hemodynamics and stroke work in elderly patients with transcervical fracture
SU Han1 LEI Jinlai2
Objective To explore the effect of continuous sputum injection of Methoxamine Hydrochloride Injection assisted intravenous-inhalation combined with general anesthesia on intraoperative hemodynamics and stroke work in elderly patients with transcervical fracture. Methods From February 2017 to February 2018, 82 patients with transcervical fracture treated by hip arthroplasty in Xi′an Red Conference Hospital were selected and divided into the control group (n = 41) and the observation group (n = 41) by the random number table method. Both groups were treated with intravenous-inhalation combined general anesthesia. The control group was given continuous infusion of normal saline before induction of anesthesia, and the observation group was given continuous pump injection of Methoxamine Hydrochloride Injection before induction of anesthesia. The hemodynamic parameters including mean arterial pressure (MAP), heart rate (HR) at the time of 5 min before anesthesia induction (T0), endotracheal intubation (T1) and 5 min (T2) and 15 min (T3) after trachea cannula, and the cardiac performance index including left ventricular beats (LVSW), stroke index (SVI) at the end of anesthesia induction and at the end of operation of two groups were compared. And the adverse reactions of two groups were compared. Results There was no significant difference between two groups in MAP and HR at T0 (P ＞ 0.05). There was no significant difference in MAP and HR at T1, T2 and T3 compared with those at T0 in the observation group (P ＞ 0.05). The MAP at T1, T2 and T3 of observation group was higher than that of control group, while HR was lower than that of control group (P ＜ 0.05). The LVSW and SVI of the observation group were lower than those of the control group at the end of anesthesia induction and at the end of operation (P ＜ 0.05). The incidence of adverse reactions in the observation group was 9.76%, lower than 29.27% in the control group (P ＜ 0.05). Conclusion Continuous pump injection of Methoxamine Hydrochloride Injection assisted intravenous-inhalation combined general anesthesia in treating elderly patients with transcervical fracture can stabilize hemodynamics in patients and reduce cardiac work, reduce the occurrence of adverse reactions such as hypotension and tachycardia.
2018 Vol. 15 (31): 99-102 [Abstract] ( 262 ) HTML (1 KB)  PDF (551 KB)  ( 137 )
103 Comparison of the anesthetic effect and hemodynamic changes of Mivacurium Chloride and Cisatracurium Besylate in ear-nose-throat surgery
ZHANG Xiaodong WANG Huiming QI Jiao SHANG Ying WEN Jing
Objective To compare the anesthetic effect and hemodynamic changes of Mivacurium Chloride and Cisatracurium Besylate in ear-nose-throat surgery. Methods From January 2017 to January 2018, 80 patients with planed operation of ear, nose and throat under general anesthesia in the Second Hospital of Baoding were divided into observation group and control group according to random number table, 40 cases in each group. Patients were given muscle relaxants for general anesthesia induction, and the control group was given the Cisatracurium Besylate, the observation group was given Mivacurium Chloride. The MAP, HR, SpO2, BIS and four TOF value at seven time points of the home invasion (T1), anesthetic induction immediately (T2), intubation (T3), end of the surgery (T4), consciousness recovery (T5), tube drawing (T6), leaving the room (T7) were recorded. The time of recover, open eyes, extubation, and the restlessness and complications of patients were recorded. Results There were no significant differences in MAP, HR, and SpO2 between the two groups from T1 to T7 (P > 0.05). Compared with T1 and T2 of the same group, the levels of SpO2 in the control group at T5, T6 and the observation group at T7 decreased, and the differences were statistically significant (P < 0.05). Compared with the control group, the BIS values at T4, T7 and the TOF values at T4 to T7 were increased in the observation group, and the differences were statistically significant (P < 0.05); the BIS and TOF values of other time points between two groups were not statistically significant (P > 0.05). Compared with T1 and T2 of the same group, TOF values of T4, T5, T6, T7 in the control group and T4, T5, T6 in the observation group decreased, and the BIS of T3, T4, T5 in the two groups decreased and the differences were statistically significant (P < 0.05). The time of recovery, open eyes, extubation in observation group were shorter than those in control group, the incidence of extubation after agitation and complications was lower than those in control group, the differences were statistically significant (P < 0.05). Conclusion The effects of Mivacurium Chloride on hemodynamics perioperative anesthesia is small, work quickly, short induction time, fast recovery of muscle contraction function, and without the effect of neuromuscular blockade accumulation, it has less postoperative muscle relaxant residual, and is suitable for the ear, nose and throat surgery, safe and effective, which is worth popularizing clinically.
2018 Vol. 15 (31): 103-106 [Abstract] ( 265 ) HTML (1 KB)  PDF (554 KB)  ( 95 )
107 Effect of ultrasound guided femoral nerve catheter on the of analgesia and delirium in elderly patients after unilateral total knee arthroplasty
WANG Qian1 DONG huiling1 ZHANG Chong1 QI Yinghua1 CHEN Qingguo1 ZHANG Changqing2 LIU Yongjian1
Objective To observe the effect of ultrasound guided femoral nerve catheter on the of analgesia and delirium in elderly patients after unilateral total knee arthroplasty. Methods From January 2015 to July 2017, 300 elderly patients with single knee replacement in the No.4 People′s Hospital of Hengshui City were divided into observation group, group A and group B according to random number table method, with 100 cases in each group. Patients in the observation group were treated with ultrasonic guided catheterization under the guidance of ultrasound, patients in group A were given blind detective femoral catheterization after the operation, and the patients in group B were given sustained intravenous analgesia. The postoperative analgesia, sedative, delirium and adverse reactions of each group were recorded. Results Compared with the group A and B, the VAS score of the observation group was significantly lower (P < 0.05), the degree of sedation was not significantly different (P > 0.05), the incidence of delirium was significantly decreased (P < 0.05), the incidence of adverse reactions, such as nausea, vomitin after operation decreased (P < 0.05). Conclusion Compared with the traditional blind detective catheterization and continuous intravenous analgesia, ultrasound guided femoral nerve catheterization improves the postoperative analgesic effect and reduces the incidence of postoperative delirium and adverse reactions, such as nausea, vomiting.
2018 Vol. 15 (31): 107-110 [Abstract] ( 267 ) HTML (1 KB)  PDF (585 KB)  ( 118 )
111 The influence of laparotomy hepatectomy patients after operation by Ropivacaine continue incision infiltration anesthesia
YU Peng1 LI Jiajun2 YIN Tianxiang3
Objective To investigate the effect of laparotomy hepatectomy patients after operation by Ropivacaine continue incision infiltration anesthesia. Methods A total of 100 patients with Ropivacaine continue incision infiltration anesthesia who were admitted to the Department of Anesthesiology, Yichang Central People′s Hospital from January 2017 to March 2018 were selected and divided into control group (salt incision infiltration) and observation group according to different anesthesia methods. The pain score of different time points after operation for two groups laparotomy hepatectomy patients were detected; the alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum albumin (ALB), and the serum albumin (ALB), direct bilirubin (DBIL), total bilirubin (TBIL), prothrombin time (PT), activated partial thrombin time (APTT) of two groups before and 3 d after operation were detected; the hospital stays after operation, the first venting time, the first cacation time, the first get out bed time, intravenous analgesic drugs, nausea, emesis, incision infection rate of two groups were observed. Results The pain score of detection group patients 6, 12, 24 h, and 48 h after operation were lower than control group, the differences were statistical significance(P < 0.05). The ALT, AST, ALB, DBIL, TBIL, PT, and APTT of two groups patients before operation and 3 d after operation were compared, the differences were no statistical significance (P > 0.05). The ALT, AST, PT, APTT of two groups 3 d after operation were higher than before operation in the same group, the hospital stays after operation, the first venting time, the first cacation time, the first get out bed time of observation group patients were shorter than control group, intravenous analgesic drugs was less than control group, the differences were statistical significance (P < 0.05). The nausea, emesis, incision infection rate of two groups patients were compared, the differences were no statistical significance (P > 0.05). Conclusion The application of laparotomy hepatectomy by Ropivacaine continue incision infiltration anesthesia, which can reduce the pain of patients, have little effect on liver function and coagulation function, and increase therapeutic effect, it is worth to be used.
2018 Vol. 15 (31): 111-114 [Abstract] ( 227 ) HTML (1 KB)  PDF (596 KB)  ( 126 )
115 Study on the medication rules of LIU Weisheng in the treatment of primary bronchogenic carcinoma of lung based on the human-computer cooperation method
CAI Yun1 CHEN Yuanbin2 WU Lei2 DENG Qingping2 LIN Lin2▲
Objective To explore the medication rules of Professor LIU Weisheng in treating primary bronchogenic carcinoma of lung ("lung cancer" for short). Methods Lung cancer cases treated at Professor LIU′s clinic in Guangdong Provincial Hospital of Chinese Medicine from 2009 to 2014 were collected. The frequency of symptoms, tongue and pulse using in diagnosis were counted. The frequency and respective ratio of surgery, radiotherapy or chemotherapy, cancer metastasis along with the specific metastasis part were calculated and the frequency of treating herbs were counted, and differences of rules among the different treating method were compared and analyzed. The results were revised and supplemented according to special interviews on Professor LIU and pertinent reference literature. Results Totally, 408 cases were included and the most common symptoms were: cough, insomnia, expectoration, asthma, etc. The colors of tongue were mostly dark red, pale and red; most of the tongue moss were thin-yellow, white-greasy and yellow-greasy. The pulses were mainly string, thin, and slippery. The most common parts of cancer metastasis were lymph gland, bone and brain. Conclusion The basic pathogenesis of lung cancer in the cases of Professor LIU is considered asthenia in origin and excess in superficiality. Herbs of supplementing Qi and nourishing Yin are mainly used in the patients after surgery while herbs of resolving phlegm and stasis are commonly used in the patients who have taken radiotherapy or chemotherapy. The method of supplementing kidney is emphasized in the treatment of bone metastasis of lung cancer while the method of resolving phlegm and wind, invigorating the circulation of blood and resolving stasis is often used in brain metastasis.
2018 Vol. 15 (31): 115-118,123 [Abstract] ( 252 ) HTML (1 KB)  PDF (675 KB)  ( 161 )
119 Realistic difficulties of traditional Chinese medicine culture propagation and countermeasures
FU Wendi
The inheritance and development of traditional Chinese medicine is highly valued by the government and it has been a national strategy. Nowadays, it has made brilliant achievements in confidence, overseas propagation, education, modernization of traditional Chinese medicine and traditional Chinese medicine culture entering the campus. However, there is the gap between the propagation and development of traditional Chinese medicine, request of strategy of cultural power, the requirements from people, national strategy and historical mission. Some development problems still exist in the aspects of core values, cross culture communication, communication platform construction, optimization of the legal environment, traditional Chinese medicine education innovation, publicity team construction and so on. In new era, culture ideal, traditional Chinese medicine confidence and its internal value should be insisted on and some countermeasures should be taken, including raising awareness, talent training, theoretical research, campus infiltration, cultural exchange, standardized construction, brand effect and new media publicity, to realize the innovative development of traditional Chinese medicine culture.
2018 Vol. 15 (31): 119-123 [Abstract] ( 319 ) HTML (1 KB)  PDF (582 KB)  ( 770 )
124 Analysis on the treatment of erectile dysfunction of diabetes by Chinese medicine dietary therapy
WANG Xuyun1 SHANG Jianwei2 SHENG Wen2 WU Songli2 WANG Ying3 YANG Liu3
Incidence of diabetes mellitus complicated with erectile dysfunction is high. Kidney deficiency and blood stasis is confirmed as its basic pathogenesis. The reasonable diet therapy around this pathology has certain curative effect. Traditional Chinese medicine holds that the principle of erectile dysfunction dietary therapy is the diet on the basis of diabetes dietary therapy. When the drug has removed most of the symptoms, dietary therapy intervenes to remove the last pathogens. The basic treating principle is differentiation of food, balanced diet, regular diet, integrated Chinese and Western diet. So that dietary therapy will play a role to a extent and improve the therapeutic effect.
2018 Vol. 15 (31): 124-126 [Abstract] ( 286 ) HTML (1 KB)  PDF (569 KB)  ( 172 )
127 Differential diagnostic value of gadoxetic acid disodium enhanced 3.0T MRI for hepatic nodules in patients with liver cirrhosis
DONG Liwei WANG Fei LUO Shishi ZHAO Yingman CHEN Feng LI Jianjun
Objective To investigate the differential diagnostic value of gadoxetic acid disodium (Gd-EOB-DTPA) enhanced MRI for hepatic nodules in patients with liver cirrhosis. Methods From February 2015 to February 2017, in Hainan General Hospital, 43 patients with liver cirrhosis were selected, and they were given Gd-EOB-DTPA enhanced 3.0T dynamic MRI and conventional Gd-DTPA enhancement. The characteristics of the images in various phases were analyzed, the results of surgical specimen pathology were as gold standard,  the test sensitivity and specificity of Gd-EOB-DTPA were evaluated. Results Gd-EOB-DTPA enhanced 3.0T MRI imaging could detected malignant nodules in the liver- specific stage, which were not found by conventional Gd-DTPA enhancement imaging. Compared with conventional Gd-DTPA enhancement, Gd-EOB-DTPA enhanced MRI detected more malignant nodules and it was not better than Gd-DTPA enhancement in detecting benign nodules. During the Gd-EOB-DTPA enhanced liver-gladder phase, most lesions appeared low signal intensity, and others appeared equal or high signal intensity (P < 0.01). Gd-EOB-DTPA enhanced MRI was better than Gd-DTPA enhancement in detecting malignant nodules (P < 0.01). The specificity of Gd-EOB-DTPA enhanced MRI was 100.00%, and the sensitivity was 93.18%. Conclusion Gd-EOB-DTPA enhanced MRI has high sensitivity and speeificity for the diagnosis of benign and malignant of liver′s abnormal perfusion nodules, and has differential diagnostic value.
2018 Vol. 15 (31): 127-130 [Abstract] ( 269 ) HTML (1 KB)  PDF (728 KB)  ( 214 )
131 Application of color Doppler ultrasound and ultrasound elastography in diagnosis of lymphatic metastasis of breast cancer
JIN Qiang1 ZHENG Xia1 SHAO Xia2
Objective To investigate the application of color doppler ultrasound and ultrasound elastography in diagnosis of lymphatic metastasis of breast cancer. Methods From January 2017 to March 2018, in Huzhou Central Hospital, 90 patients with breast cancer were selected, they were detected by color Doppler ultrasound and ultrasound elasticity imaging on preoperative and histological pathology examination postoperative. Results Among 90 patients, 49 patients were lymphatic metastasis, and 41 patients without lymphatie metastasis. The color Doppler flow grade and score of ultrasound elasticity imaging in the patients with lymphatic metastasis were significantly higher than the patients without lymphatic metastasis, the differences were statistically significant (P < 0.05). The sensitivity and specificity of color doppler ultrasound combined with ultrasonic elastography were significantly higher than the color Doppler ultrasound and ultrasonic elastography, the differences were statistically significant (P < 0.05). Conclusion Color Doppler ultrasound and ultrasound elastography, were not the same for patients with lymphatic metastasis of breast cancer and non-metastic patients and the ultrasonic elastography has high sensitivity and specificity for lymphatic metastasis of breast cancer. It is worthy of clinical popularization and application.
2018 Vol. 15 (31): 131-134 [Abstract] ( 274 ) HTML (1 KB)  PDF (561 KB)  ( 97 )
135 Clinical research on MRI evaluating right heart structure and function in patients with chronic heart failure
LI Yan HUANG Lingbo FU Bing YANG Zhi ZHAO Zhiyong WANG Bo
Objective To study the value of cardiac magnetic resonance (CMR) in the evaluation of right heart structure and function in chronic heart failure. Methods From January 2016 to December 2017, in the Fifth People′s Hospital of Chengdu, 32 patients with CHF were chosen as observation group, another 32 healthy person who received physical examination at the same period were chosen as control group. CMR examination was performed in both groups, and the right heart structure and function indexes of two groups were compared, such as right ventricular thickness (RVT), right ventricular mass (RVM), right ventricular papillary muscle mass (RVPM), right ventricular end diastolic volume (RVEDV), right ventricular systolic volume (RVESV), right ventricular stroke volume (RVSV), right ventricular ejection fraction (RVEF). Results The RVT, RVM, RVPM, RVEDV, RVESV and RVSV of the right ventricle in the observation group were significantly higher than those in the control group, the RVEF was significantly lower than that in the control group, the differences were statistically significant (P < 0.05 or P < 0.01). Conclusion CMR can quantitatively analyze the right ventricular structure and function of patients with CHF, which helps to determine the patients′ disease progression.
2018 Vol. 15 (31): 135-137 [Abstract] ( 232 ) HTML (1 KB)  PDF (524 KB)  ( 58 )
138 Comparative study of imaging and pathological manifesations of inflammatory myofibroblastic tumor
LIAO Jiang CHEN Jiayou ZHENG Xiang FANG Yanhong XU Shugui RAO Yanying CHEN Ying▲
Objective To investigate the image features combined with pathological changes of inflammatory myofiroblastic tumor (IMT), in order to improve diagnostic level. Methods From May 2008 to December 2017, in Fujian Province Tumor Hospital, the CT and MRI findings of 11 patients with IMT confirmed by histopathology on surgically resected specimen were retrospectively. The location, size, shape, density or signal, and contrast enhancement of these tumors were analyzed. Results In 11 cases of IMT, 3 cases occurred in lung, 2 cases oceurred in stomach, abdominal cavity, abdominal wall, mesentery, bladder, ureter and leg soft tissue was 1 case respectively. Tumor characterized by solid soft tissue mass 7 cases, 4 cases of cystic or solid mass. The density or signal of the most tumor was uneven, and 1 case lesion could be seen calcification. The majority of cases had a clear boundary. After contrast administration, moderate or marked homogeneous or heterogeneous enhancement, it was found. IMT occurred in abdominal cavity, abdominal wall and mesentery had invasive imaging features. The tumors were mainly composed of spindle shaped fibrous cells and inflammatory cells on pathology, and positive staining for muscle deriving proteins was observed immunohistochemically. The wave type protein (Vimentin) was positive in 10 patients, the smooth muscle actin (SMA) was positive in 8 patients, the muscle actin (MSA) was positive in 5 patients, and ALK, S-100 protein, CD117 and CD34 were all negative. Conclusion IMT is a rane tumor, and its imaging features hawe certain value for localization and qualitation diagnosis, but the diagnosis stiu needs to rely on pathological examination.
2018 Vol. 15 (31): 138-141,154 [Abstract] ( 290 ) HTML (1 KB)  PDF (1103 KB)  ( 123 )
142 Ultrsonographic appearances of ovarian thecoma and diagnosis analysis
WANG Guanjie CAO Yunyun GONG Xiaoping WANG Haifei YANG Tian KONG Xiaoxiao WANG Yamei NIU Jianmei▲
Objective To study ultrasonographic appearances and clinical features of ovarian thecoma, in order to raise its diagnosis rate. Methods From January 2015 to October 2016, in International Peace Maternity and Child Health Hospital, the clinical related data and ultrasonographic appeareaces of 47 patients with ovarian thecoma proved by pathology were retrospectively analyzed. Results 95.7% (45/47) of all the tumors were unilateral lesions (26 cases were on the left, 19 cases were on the right). 72.3% (34/47) of the patients showed no obviously clinical symptoms. 86.7% tumors appeared hypoechoic echo. 51.1% (23/45) of the tumor boundaries appeared unclearly or indistinctly. Posterior echo attenuation rate was 44.4% (20/45). Color doppler flow imaging showed that the blood flow signal could be detected in 37.8% (17/45) tumors. The ultrasound detection rate was 95.7% (45/47). Positioning accuracy rate was 100.0% (45/45). Misdiagnosis rate was 17.8% (8/45). Missed diagnosis rate was 4.3% (2/47). The clinical symptoms of the tumor were not specific and could be misdiagnosed as out projecting uterine myoma, ovarian fibroma or malignancy ovaria tumor. Conclusion By mastering the ultrasonographic appearances and clinical characteristics of ovarian thecoma, it is expected to improve the accuracy of ultrasound diagnosis and reduce missed diagnosis and misdiagnosis.
2018 Vol. 15 (31): 142-145 [Abstract] ( 276 ) HTML (1 KB)  PDF (735 KB)  ( 65 )
146 Systematic review on effects of prophylactic use of antibiotics in lateral perineal incision
BAI Xue YANG Youyun HAN Feng HUANG Jinyue
Objective To systematically evaluate the relevance between the prophylactic use of antimicrobial drugs and the incision infection after episiotomy, and to guide rational medication in clinic. Methods PubMed, Embase, Cochrane Library, Chinese Biomedical Literature Database, Chinese Academic Journal Full-text Database and Wanfang Database were used for searching all relevant literatures since the libraries were built up to December 2017, and the studies of correlation between prophylactic use of antimicrobial drugs and incision infection after episiotomy were collected. RevMan 5.3 software was used for Meta-analysis. Results A total of 15 articles were included with 4355 patients. Meta-analysis showed that prophylactic use of antimicrobial drugs was associated with the incision infection after episiotomy[OR = 0.50, 95%CI (0.35, 0.71), P = 0.0001]. Subgroup analysis about the modes of action of antimicrobial drugs suggested that the use of focal irrigation had a relevance to the incision infection after episiotomy [OR = 0.25, 95%CI (0.12, 0.53), P = 0.0003], while there was no significant association between systemic application and incision infection [OR = 0.78, 95%CI (0.48, 1.26), P = 0.31]. Conclusion Prophylactic use of antimicrobial drugs may reduce the incidence of incision infections after episiotomy, especially in focal irrigation applications. However, due to the limited number of documents and sample size, large-sample, multi-center and well-designed clinical trials are still needed to confirm and improve the conclusions of this study.
2018 Vol. 15 (31): 146-150 [Abstract] ( 283 ) HTML (1 KB)  PDF (1089 KB)  ( 114 )
151 Comparation of disease assessment and prediction efficacy of CAT scoring system and CAPS scoring system in patients with AECOPD
Hadiya·aierke Dilinuer·wupusu YAN Fang Muyeshaer·pidayi HE Yuanbing▲
Objective To compare the disease assessment and prediction efficacy of CAT (chronic obstructive pulmonary disease assessment test) score system and CAPS (the chronic obstructive pulmonary disease and asthma physiology score) system on patients with AECOPD (acute exacerbation of chronic obstructive pulmonary disease). Methods Prospective analysis was adopted, 117 patients with AECOPD from a first-class hospital in Urumqi were received from July 2014 to August 2017 were selected, 105 cases were survived and 12 cases were died. All patients were evaluated by CAT score and CAPS, and statistical SPSS and MedCalc software were used to analyze these evaluation index, and the ROC curve analysis. Results The sensitivity of CAT score was 88.12%, specificity was 75.53%, positive likelihood ratio was 3.89, negative likelihood ratio was 0.11, Youden index was 0.698; the sensitivity of CAPS score was 74.32%, specificity was 63.57%, positive likelihood ratio was 2.15, negative likelihood ratio was 0.31, Youden index was 0.439; there was statistically significant difference in the CAT and CAPS score between death and survival patients (P < 0.05). The area under the curve (AUC) of CAT score was 0.648, the AUC of CAPS score was 0.680, and the comparison of the AUC of two scores was statistically significant (P < 0.05). Conclusion Compared with the CAPS score, CAT score can be used to evaluate the patients with AECOPD in a rapider, more specific and more efficient disease assessment method, which has a good predictive value and guiding significance.
2018 Vol. 15 (31): 151-154 [Abstract] ( 271 ) HTML (1 KB)  PDF (404 KB)  ( 72 )
155 Research on management information platform of scientific research reagent consumables in public hospitals based on business process reengineering theory
ZHANG Yi LIU Yuping CHEN Lianghua LIU Yuanjie YANG Weihong
The establishment of the management model for the informatization of scientific research consumables in hospitals is an important part of the realization of digital hospital construction. It is of great significance in improving the efficiency of scientific research activities, standardizing the procurement process of consumables, and avoiding the corruption of scientific researchers. Aiming at the blueprint for the development of public hospital management informatization under the direction of the new medical reform policy, we analyze the status quo of reagents and supplies procurement management in hospital research institutes, point out the insufficiency in the management of reagent consumables in domestic hospitals, explore the reconstruction of scientific research reagent consumables procurement process and the research is in line with the implementation strategy of scientific reagent consumables management information system in the First Hospital Affiliated to Guangzhou Medical University. It provides reference for domestic hospitals and medical institutions at all levels to establish a scientific research reagent consumables management information platform.
2018 Vol. 15 (31): 155-159 [Abstract] ( 295 ) HTML (1 KB)  PDF (762 KB)  ( 116 )
160 An analysis on the construction of humanistic hospital in public hospitals under the background of "Internet + Medicine"
JIN Feng1 SUN Hongliang2 REN Longxi3
Humanistic hospital is a sustainable development hospital with the core value of hospital culture construction. The traditional "extensive" hospital development does not pay attention to the construction of hospital culture and the role of human in organization. While the background of "internet + medicine" contributes to the construction of hospital informatization, it also provides a new starting point and platform for the construction of hospital culture. Based on the background of "internet + medicine", this paper analyzes the construction of humanistic hospitals and explores the theoretical basis and path of the construction of humanistic hospitals in public hospitals.
2018 Vol. 15 (31): 160-163 [Abstract] ( 298 ) HTML (1 KB)  PDF (519 KB)  ( 112 )
164 Analysis of the current situation and countermeasures of intangible assets management in public hospitals under the new situation
WANG Yiru
In order to further realize financial fine accounting, improve the level of hospital economic management. From the actual case, this paper expounds the status of the management of the intangible assets in the public hospitals in recent years, and points out that there are some outstanding problems in the management of the intangible assets of the hospital, such as the indifference of management consciousness, the unsound accounting system and the imperfect management. In view of the above problems, this paper puts forward multiple measares to strengthen the management consciousness of intangible assets, the construction of the work system of intangible assets management, the scientific selection of intangible assets evaluation method, the promotion of the application of intangible assets, the construction of the concept of goodwill and so on in order to promote the efficient and orderly development of the management of the intangible assets in the hospital.
2018 Vol. 15 (31): 164-167 [Abstract] ( 168 ) HTML (1 KB)  PDF (518 KB)  ( 85 )
168 Status, analysis and countermeasures of community nursing staff in Xiamen
DAI Qingxia
The community nursing teams in Xiamen have existed many problems in development: insufficiency of personnel, lack of occupation stability, team aging, unreasonable structure, complicated task request, lack of further education, etc. This article aims at through an practical investigation and learning advanced experience from developed countries, from the perspective of serious aspects including talents resource, entry qualification, further education and pay, putting forward reasonable measures to improve the dilemma and overall strength, so that it will promote the integral improvement of community health service.
2018 Vol. 15 (31): 168-171,180 [Abstract] ( 234 ) HTML (1 KB)  PDF (498 KB)  ( 99 )
172 Application of collaborative cognitive behavioral nursing in patients with chronic kidney disease
HUO Tingting REN Xiaodong YANG Xiaojing LENG Yuqing
Objective To investigate the application of collaborative cognitive behavioral nursing in patients with chronic kidney disease (CKD). Methods A total of 120 patients with CKD treated in the First Affiliated Hospital of Hebei North University from May 2015 to May 2017 were collected and divided into observation group and control group according to the random number table method, with 50 cases in each group. The observation group was treated with collaborative cognitive behavioral nursing and the control group was treated with conventional care. Renal function index, self-care ability and compliance before and after interlention were compared between the two groups. Results Before intervention, self-care ability containing self-care skill, self-concept, self-responsibility, health knowledge level of the two groups had no significant difference (P ＞ 0.05); after intervention, self-responsibility and health knowledge level were improved compared to those before intervention in the control group (P ＜ 0.05), self-concept, self-responsibility, health knowledge level in the observation group were improved compared with those before intervention (P ＜ 0.05); after intervention, scores of self-care skill and self-responsibility in the observation group were higher than those in the control group (P ＜ 0.05). Before intervention, there was no significant difference in the renal function between the two groups (P ＞ 0.05), while after intervention, the two groups′ renal function improved compared with those before intervention (P ＜ 0.05), and urea nitrogen (BUN), 24 h urine protein and serum creatinine (Scr) in obseroation group were lower than those in the control group (P ＜ 0.05). The total compliance rate in the observation group was 96.0%, significantly higher than that in the control group (66.0%) (P ＜ 0.05). Conclusion Collaborative cognitive behavioral nursing is helpful to improve the renal function of patients with CKD, which improves their nursing ability and the compliance, and it is suggested to be promoted promotion in clinic.
2018 Vol. 15 (31): 172-175 [Abstract] ( 258 ) HTML (1 KB)  PDF (515 KB)  ( 86 )
178 Related factors survey of self perceived burden of cancer patients and effect of supportive psychotherapy
YE Shengchang1 LIU Min2
Objective To study the clinical effect of supportive psychotherapy in improving patients′ SPB on the basis of analyzing the related factors of the self-perception burden (SPB) in cancer patients. Methods One hundred eighteen cancer cases from January 2016 to December 2017 treated in Shanghai Ninth People′s Hospital, Affiliated of School of Medicine, Shanghai Jiao Tong University were selected randomly. The patients′ general information, attitude to disease, SBP were investigated, then related factors of SBP were analyzed. All the 118 cases were divided into observation group and control group by random number table method, with 59 cases in each group. The control group was given conventional psychological interventions, and the observation group was given psychological intervention treatment on the basis of the control group. Improvement of SBP scores and attitude to disease were compared between the two groups. Results The primary caregivers being the siblings, surgical treatment, and the chemotherapy frequency≥7 times were the risk factors for the SPB scores of cancer patients (P ＜ 0.05). After psychological intervention, SBP scores in the observation group was lower than that in the control group, and active confrontation scores in the observation group was higher than that in the control group, while passive avoidance scores and disappointment to yield scores were lower than those in the control group (P ＜ 0.05). Conclusion Cancer patients are common with SPB negative experience, the negative experience relates to the intimate degree with caregivers and surgery, chemotherapy treatment concerns, and they should be given supportive psychotherapy, to ensure that cancer patients′ SPB is released, which can keep patients′ positive attitude towards the disease.
2018 Vol. 15 (31): 178-180 [Abstract] ( 266 ) HTML (1 KB)  PDF (532 KB)  ( 141 )
Copyright © Editorial Board of 中国医药导报
Supported by:Beijing Magtech