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Study on quality standard of Weiyanling Granules |
QIAN Mengxi1 ZHANG Yongmei1 KANG Zhen1 QIAN Wei1 YU Hui1 LI Jianfeng2▲ |
1.Graduate Workstation of Enterprises in Jiangsu Province, Department of Pharmacy, Zhangjiagang Hospital Affiliated to Nanjing University of Chinese Medicine, Jiangsu Province, Zhangjiagang 215600, China;
2.Department of Pharmacy, Wujin Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province, Wujin 213161, China |
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Abstract Objective To establish the quality standard of Weiyanling Granules. Methods Astragali Radix, Coptidis Rhizoma, Glycyrrhizae Radix et Rhizoma in Weiyanling Granules were identified by thin-layer chromatography (TLC). The contents of geniposide and paeoniflorin in Weiyanling Granules were determinated by high performance liquid chromatography (HPLC) simultaneously. The HPLC method was established with Diamonsil C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was consisted of methyl alcohol-water (20∶80, V/V) with the flow rate of 1 mL/min. The detective wavelength was set at 235 nm, and the column temperature was at 25℃. Results TLC spots were clear and well-separated without interference from negative sample. The linear range of geniposide and paeoniflorin were 1.030-10.300 μg and 1.032-10.320 μg with the average recovery being 98.92% and 97.75%, RSD being 1.39% and 1.19%, respectively. Conclusion The method is simple, feasible, reproducible and accurate, and is suitable for the quality control of Weiyanling Granules.
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