七子益肾理冲汤对多囊卵巢综合征胰岛素抵抗大鼠卵巢上皮细胞炎性因子及胰岛素抵抗状态的影响

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Abstract Objective To analyze the effect of Qizi Yishen Lichong Decoction on inflammatory cytokines and insulin resistance in ovarian epithelial cells of rats with polycystic ovary syndrome (PCOS) insulin resistance. Methods A total of 60 SPF Wistar male rats were selected to prepare insulin-resistant PCOS model rats. A total of 54 rats were enrolled in this study. Random number table method was used to divide them into observation group (27 rats) and control group (27 rats). The observation group was given intragastric administration of 6 mL/kg of Qizi Yishen Lichong Decoction, the control group was given intragastric administration of distilled water of equal dose, both groups were given gastric lavage once a day for 20 days. The level of serum follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (T) were detected by chemical immunofluorescence method. The content of inflammatory cytokine pigment epithelium derived factor (PEDF), Visfatin and interleukin-6 (IL-6) in ovarian epithelial cells were detected by enzyme-linked immunosorbent assay (ELISA). The levels of serum fasting insulin (FINS) and fasting blood glucose (FBG) were measured by radioimmunoassay, gastric tube glucose after 2 hours of blood glucose (OGTT-2 h BG) content was detected by glucose oxidase method, insulin resistance index (HOMA-IR) and insulin sensitivity index (ISI) were calculated. Results The levels of LH, T, LH/FSH of the observation group were lower than those of the control group (P ＜ 0.05). The contents of serum FBG, OGTT-2 h BG, FINS and HOMA-IR of the observation group were significantly lower than those of the control group, while ISI was higher than that of the control group, the differences were statistically significant (P ＜ 0.05). The contents of PEDF, Visfatin and IL-6 of the epithelial cells of the observation group were lower than those of the control group, and the differences were statistically significant (P ＜ 0.05). Conclusion Qizi Yishen Lichong Decoction can reduce the content of inflammatory cytokines in ovarian epithelial cells of PCOS insulin-resistant rats, regulate the ratio of LH/FSH, improve hyperinsulinemia and hyperandrogenism, and adjust the disorder of glucose metabolism.

Key words： Polycystic ovary syndrome Insulin resistance Inflammatory cytokine Qizi Yishen Lichong Decoction

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	CHEN Haiyan TANG Ling▲ XIAO Chengcong

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CHEN Haiyan TANG Ling▲ XIAO Chengcong. Effect of Qizi Yishen Lichong Decoction on inflammatory cytokines and insulin resistance of ovarian epithelial cells in PCOS insulin-resistant rats[J]. 中国医药导报, 2018, 15(31): 12-15.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I31/12

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