胰岛素样生长因子-1对全脑缺血大鼠学习记忆能力的影响

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Abstract Objective To investigate the effect of exogenous insulin-like growth factor-1 (IGF-1) on learning and memory ability in rats with global cerebral ischemia and its possible mechanism. Methods A total of 110 Wistar male rats were divided into normal group, sham operation group, global cerebral ischemia model group, model administration group 1 and model administration group 2; there were 10 rats in each of the normal group and sham operation group. There were 30 rats in each of the whole cerebral ischemia model group, the model drug administration group 1 and the model drug administration group 2. All operated groups were embedded micro-injection catheter into the lateral ventricle, and the whole cerebral ischemia model was established on the 6th day after catheterization with modified Pusinelli four-vessel blockade. Rats in the global cerebral ischemia model group, model drug administration group 1, and model drug administration group 2 were injected with 10 μL of normal saline, IGF-1 (0.2 μg/μL) 10 μL, and IGF-1+PPP (after intraperitoneal injection of blocker PPP 20 mg/kg for 30 min, IGF-1 (0.2 μg/μL) 10 μL was injected into the lateral ventricle), once a day for 7 d. In the sham operation group, NS 10 μL was injected into the lateral ventricle of rats. Morris water maze test was used to test the learning and memory ability of rats in each group before and after administration. HE staining was used to observe the apoptosis of hippocampal CA1 neurons. Immunohistochemistry was used to detect the expression of p-Akt and p-mTOR proteins in hippocampus. Results Compared with the global cerebral ischemia model group, the average escape latency of the model administration group 1 was significantly shortened (P < 0.05), the number of straddle platform and the original platform quadrant swimming time increased significantly (P < 0.05); the number of cells in the hippocampal CA1 area increased. The arrangement was uniform and the structure was clear; the expression of p-Akt and p-mTOR protein were higher (P < 0.05). Conclusion The learning and memory ability in rats with global cerebral ischemia was improved by exogenous IGF-1. This may be related to the inhibition of apoptosis in the hippocampal CA1 region and the enhancement of the expression of p-Akt and p-mTOR proteins in the PI3K/Akt signaling pathway.

Key words： IGF-1 Global brain ischemic Learning and memory p-Akt p-mTOR

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	LI Yongli1 ZHAO Jing2 LIU Liming1 ZHANG Quanbo1 LIU Li1 WU Bihua1

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LI Yongli1 ZHAO Jing2 LIU Liming1 ZHANG Quanbo1 LIU Li1 WU Bihua1. Effect of IGF-1 on learning and memory ability in rats with global cerebral ischemia[J]. 中国医药导报, 2018, 15(31): 8-11.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I31/8

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