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2017 Vol. 14, No. 18 Published: 2017-06-25

	4	Optimization of microbial limit test method for purified water
		SUN Linlin WANG Hailiang LIU Yiming LU Huifen WU Guoqiang
		Objective To establish a more scientific and reasonable purified water microbial limit test method. Methods Membrane filtration method (sample quantity: 1 mL and 10 mL) were used to do the test, and nutrient agar, rose bengal agar medium, plate count agar and R2A medium were used to demonstrate the microbiology separately. Results Membrane filtration method (sample quantity: 10 mL) and R2A were better than the routine method and the other medium with a much higher occurrence of total aerobic plate count. The detection rate was 1-2 times higher than the rate of other method and medium. Conclusion The selected testing sample quantity and culture medium are optimal, and the optimized test method will reflect the real quality of the purified water. The study will provide support for the testing improvement and quality control level.
		2017 Vol. 14 (18): 4-6 [Abstract] ( 266 ) HTML (1 KB) PDF (567 KB) ( 96 )

	7	Role of panax notoginoside in rats with hypoxic hypercapnia pulmonary hypertension induced by JNK signal transduction pathway
		ZHOU Xiaoxiong YE Taochun LUO Chuanjin WU Hui CHU Qingmin ZHAO Xinjun
		Objective To investigate the dynamic change of JNK signaling pathway in the occurrence and development of hypoxic hypercapnia pulmonary hypertension in rats, discuss the mechanism of panax notoginoside (PNS) in the protection of hypoxic hypercapnia pulmonary hypertension. Methods Rats pathological models of hypoxic hypercapnia pulmonary hypertension were established: SD rats were randomly divided into three groups (n=10): normal group (N group), hypoxic hypercapnia for 4 week group (H4w group), and PNS-injected group (Hp4w group). RT-qPCR and Western blot were used to detect the expression of JNK. Primary cultured PASMCs, isolated from SD rats, were incubated in logarithmic growth phase from the 2nd to 5th generation. PASMCs were divided into five groups: normal group (N group), hypoxic hypercapnia group (H group), and R1 treated group with different concentrations (10, 50 and 100 mg/L) of notoginsenoside monomer R1 under the condition of 6% CO2 plus 1% O2 for 24 h (R10, R50 and R100 groups). The expressions of JNK mRNA and protein levels were detected by RT-qPCR and Western blot. PASMCs were treated with R1 and JNK inhibitor SP600125 for 5 days, CCK8 was used to detect the proliferation of PASMCs. Results ①The mPAP in H4w and Hp4w group was higher than that of N group (P < 0.05), but mPAP in Hp4w group was obviously lower than that of H4w group (P < 0.05). ②The mRNA and protein levels of JNK were significantly increased in H4w and Hp4w groups, compared with N group (P < 0.05), and Hp4w group was lower than H4w group (P < 0.05). ③The protein and mRNA expression levels of JNK in PASMCs were significantly higher in H group than those in N group (P < 0.05); compared with H group, in R1 treatment (10, 50 and 100 mg/L) groups, the expression levels of JNK were markedly decreased (P < 0.05). ④The proliferation of PASMCs were significantly inhibited in groups treated with R1 and JNK inhibitor SP600125 (P < 0.05). Conclusion JNK may play an important role in the development of hypoxia induced pulmonary hypertension. The effect of PNS on reducing pulmonary hypertension and improving pulmonary vascular wall remodeling may be partly related to its inhibition of JNK pathway.
		2017 Vol. 14 (18): 7-11 [Abstract] ( 176 ) HTML (1 KB) PDF (817 KB) ( 49 )

	12	Effect of 1, 25 (OH)2D3 on apoptosis and VDR protein expression of human leukemia 6T-CEM cell line
		Gulibaha·Maimaiti LIU Yu ZHAO Li YAN Mei
		Objective To investigate the effect of 1, 25-dihydroxyvitamin D3 [1, 25 (OH)2D3] on apoptosis of 6T-CEM cell lines and expression of vitamin D receptor (VDR) protein human leukemia. Methods The 6T-CEM cell lines were cultured and randomly divided into group A, B, C, D. Group A, B, C were added with 1, 25 (OH)2D3 with the concentration of 10-6, 10-7, 10-8 mol/L respectively, and group D was added with DMSO. The absorbance A value and cell proliferation inhibition rate were detected by MTT method, the cell morphology was observed by AO-EB fluorescence staining, and the cell apoptosis was detected by flow cytometer, the expression of VDR mRNA and protein were detected by RT-PCR and Western blot method. Results The absorbance of group A was apparently lower than that of group B, C and D (P ＜ 0.05); the inhibitory rate of cell proliferation of group A, B, C was A>B>C; the CEM cell apoptosis index of group D was apparently lower than that of group A, B and C (P ＜ 0.05); the apoptosis index of group A was apparently higher than other three groups (P ＜ 0.05); there was no statistically significant difference in VDR mRNA expression among the four groups (P ＞ 0.05). The VDR protein expression of group A was apparently higher than that of other three groups (P ＜ 0.05). Conclusion 1, 25 (OH)2D3 can effectively inhibit the proliferation of 6T-CEM cells, induce its apoptosis, up-regulate the VDR protein expression.
		2017 Vol. 14 (18): 12-15 [Abstract] ( 143 ) HTML (1 KB) PDF (611 KB) ( 13 )

	16	Peimine inhibits cell proliferation in human leukemia cell line K562 through redox imbalance
		QI Yan LIAO Bin XU Chengbo HUANGFU Zhenping CHEN Jiawei
		Objective To investigate the effect of peimine on cell proliferation of human leukemia K562 cells and the function of redox imbalance in this progress. Methods The human leukemia K562 cells were cultured in vitro. After K562 cells were treated with peimine at concentrations of 50, 100, 200, 400 and 800 μmol/L for 24 h, the cell proliferation was measured by thiazolyl blue tetrazolium bromide (MTT) assay. And the half maximal inhibitory concentration (IC50) of peimine was calculated using the Bliss method. The changes of intracellular reactive oxygen species (ROS) and glutathione (GSH) concentration were detected using spectrophotometry. Results Peimine inhibited the proliferation of K562 cells in a dose-dependent manner. And the IC50 of peimine was 238 μmol/L. Furthermore, peimine could promote the ROS production and glutathione (GSH) depletion. The balance of oxidation-antioxidation function destroyed. Pre-incubation with antioxidants GSH or N acetyl cysteine (NAC) almost abolished the effects of peimine. Conclusion The present study therefore shows that peimine inhibits the proliferation activity in K562 cells. The redox imbalance may play a key role in this process.
		2017 Vol. 14 (18): 16-19 [Abstract] ( 202 ) HTML (1 KB) PDF (728 KB) ( 87 )

	20	Protective effect of naringenin pretreatment on isolated heart ischemia/reperfusion injury in rat
		MENG Limin1 WU Shaohua2 XI Shuqiang3 YANG Hongliang1 ZHANG Yi4▲
		Objective To investigate the protective effect of naringenin on myocardial ischemia/reperfusion injury in isolated rat hearts. Methods 56 SD rats were randomly divided into: normal control (CON) group, naringenin (NARI) of 1, 2.5, 5, 10, 20, 40 μmol/L groups. After balance perfusion for 30 min in each group, CON group was treated with 30 min global ischemia followed by 60 min reperfusion (or 120 min for determination of the infarct size). The NARI rats hearts were pretreated with 1, 2.5, 5, 10, 20, 40 μmol/L Nari perfusion for 10 min before ischemia, and then 30 min global ischemia and 60 min reperfusion (or 120 min for determine the infarct size). The left ventricular function was obseved; levels of myocardial lactate dehydrogenase, myocardial superoxide dismutase (SOD) activity and malondialdehyde (MDA) were measured; and the percentage of myocardial infarct size was calculated in each group. Results Compared with the CON group, the recovery of heart function was increased, the content of lactate dehydrogenase and the area of myocardial infarction were decreased after ischemia/reperfusion in 2.5 μmol/L and above NARI pretreatment group (P < 0.05 or P < 0.01). Compared with the control group, the SOD activity of myocardium significantly increased and the content of MDA significantly decreased in 2.5 μmol/L and above NARI pretreatment group(P < 0.05 or P < 0.01). Conclusion Naringenin has protective effect for isolated rats hearts with ischemia/reperfusion injury, its mechanism may be associated with enhanced myocardial antioxidant capacity.
		2017 Vol. 14 (18): 20-24 [Abstract] ( 161 ) HTML (1 KB) PDF (1071 KB) ( 81 )

	25	Butein induces EC109 cell apoptosis via activating AMP-activated protein kinase/forkhead class box O3a pathway and cellular oxidative stress
		CHEN Shaoyang YUE Honglin LIU Xu
		Objective To investigate the effects of Butein on esophagus cancer EC109 cells and the role of AMP-activated protein kinase (AMPK)/forkhead class box O3a (FOXO3a) and oxidative stress in this process. Methods EC109 cell was routine cultured and given Butein, the cell vitality, migration ability, cellular oxidative stress level of EC109 cell and Caspase 3 activity were detected. The AMPK, FOXO3 aphosphorylation level and the expressions of Bim and Bax were detected by Western blot. After Compound C inhibit AMPK, related indicators were detected. The nude mice tumor bearing model was made, Butein and Compound C were given, the weight and tumor volume of nude mice were detected. Results After Butein treatment, cell vitality reduced, the distance between cell boundaries increased, ROS concentration and NADPH oxidase activity increased, total GSH level and Caspase 3 activity reduced (P < 0.05); AMPKand FOXO3 aphosphorylation and cell apoptosis increased (P < 0.05); Compound C treatment reversed this process. The nude mice tumor study showed that Butein inhibited the tumor growth and Compound C reversed this effect. Conclusion Butein can induce EC109 cell apoptosis, and this process may be mediated by activation of AMPK/FOXO3a and cellular oxidative stress.
		2017 Vol. 14 (18): 25-29 [Abstract] ( 170 ) HTML (1 KB) PDF (1135 KB) ( 128 )

	30	Research progress of traditional Chinese medicine in regulating the DNA methylation
		TANG Chenchen LIU Yamin SHI Yulin CHEN Qilong
		Epigenetics is a genetic change of the organism phenotype or gene expression, but its alterations do not involve the changes of DNA sequences, which emphasizes the interaction between genes and environment, and it has been becoming a hot content in the frontier research of life sciences. As one content of the research progress of epigenetics and a kind of reversible modification in response to internal and external environmental changes, the abnormal of DNA methylation pattern is associated with many diseases. Previous studies show that the traditional Chinese medicine plays a role for disease treatment by DNA methylation modification. This paper summarizes the recent studies of traditional Chinese medicine in regulating the DNA methylation from the aspects of different drug properties of traditional Chinese medicine, traditional Chinese medicine monomer and Chinese compound formula, so as to provide theoretical basis for further studying the targets and mechanism of traditional Chinese medicine in the process of treatment.
		2017 Vol. 14 (18): 30-33 [Abstract] ( 193 ) HTML (1 KB) PDF (599 KB) ( 92 )

	34	Research progress on influencing factors and intervention strategies of family members' uncertainty of patients in ICU
		LI Na1 GUAN Hong2
		Influence factors of uncertainty in illness of family members mainly includes family sex, education level、economic level、relationship with patients，the patient's condition changes and patients entered the ICU. Related factors include social support, coping style, quality of life, care needs. The strategies reduce families' uncertainty mainly includes improving the visit system, meeting the needs of families to visit; actively communicate with their families, to win the families′ trust; mobilizing the social support system, and actively encouraging families should cope positively; diversified education, provide information support, so as to provide reference for the implementation of the interventions.
		2017 Vol. 14 (18): 34-37,52 [Abstract] ( 157 ) HTML (1 KB) PDF (668 KB) ( 83 )

	38	Research progress of effect of glucagon-like peptide-1 receptor agonists on vascular complications in patients with type 2 diabetes mellitus
		LIU Jingjing1 WEI Li2 WANG Jianbo2
		Type 2 diabetes is a major risk factor of atherosclerotic disease. Thus, the prevention and treatment of diabetic vascular lesions is another important purpose in addition to hypoglycemic for diabetes patients. Exogenous administration of glucagon-like peptide-1 (GLP-1) or GLP-1 receptor agonist is a new kind of antidiabetic drugs, its hypoglycemic effect has been very clear, mainly through the combination with GLP-1 receptors, thereby promoting insulin secretion to reduce blood glucose. In recent years, domestic and foreign studies have found that GLP-1 receptor agonists also have cardiovascular protection. The mechanism may be achieved by improving the function of vascular endothelial cells, antioxidant stress, lowering blood pressure, lowering blood lipid, inhibiting the apoptosis of myocardial cells and so on. However, whether GLP-1 receptor agonists can improve the development of vascular complications in patients with T2DM remains to be confirmed for a long time.
		2017 Vol. 14 (18): 38-41 [Abstract] ( 190 ) HTML (1 KB) PDF (601 KB) ( 99 )

	42	Research progress on the pathogenesis and TCM therapy of coagulation disorders in sepsis
		XU Zhibing1 SONG Li1 ZHAO Wairong2
		Sepsis is life-threatening organ dysfunction caused by a deregulated host response to infection, which is critical and can develop into septic shock. There is an inseparable relationship between coagulation disorders and the degree of disease severity and prognosis in patients with sepsis, coagulation disorders runs through the whole pathological process of sepsis. This article mainly reviews the pathogenesis and TCM therapy of coagulation disorders in sepsis, so as to provide new ideas and methods for treating sepsis in clinic.
		2017 Vol. 14 (18): 42-44 [Abstract] ( 190 ) HTML (1 KB) PDF (630 KB) ( 203 )

	45	Application progress of Etomidate
		YANG Tianqi WANG Hui
		Etomidate is a kind of nonbarbiturate intravenous anesthetic. Due to the unique pharmacological characteristics, Etomidate is especially suitable for the shock, heart disease, high blood pressure and the anesthesia of elderly patients. For a long time, the inhibition effect of Etomidate on the adrenocortical function of the patients limits its application scope. In recent years, with the continuous study of Etomidate, it is found that the inhibition effect on the adrenal cortex is reversible, and a large number of clinical trials have confirmed that Etomidate can be used to maintain the normal adrenal function, and the anesthesia for the patients with the operation time less than 3 hours. At the same time, the study of new dosage forms of Etomidate will broaden the clinical indications of Etomidate.
		2017 Vol. 14 (18): 45-48 [Abstract] ( 177 ) HTML (1 KB) PDF (638 KB) ( 92 )

	49	Research progress of minipigs in reproductive toxicity studies
		ZHANG Yuanhui GAO Hong▲
		As to anatomy and pathophysiology, there are lots of similarities between human and minipig. Because of its short estrus cycle and large number of piglets in a litter, minipig has become an ideal animal model for medical research in human. However, minipig has been restrictedly used in reproductive toxicity studies because of its large body size and limited background data. In this review summarizes the characteristics of minipig as well asits current applications in reproductive toxicity studies, intend to expand the range of selecting non-rodents and provide new ideas for nonclinical reproductive toxicity studies.
		2017 Vol. 14 (18): 49-52 [Abstract] ( 200 ) HTML (1 KB) PDF (602 KB) ( 142 )

	53	Research of status and effect factors of ICU nurses' knowledge, attitudes and practices about sedation in Shiyan
		WANG Rong YANG Baoyi LI Yaling DENG Chunyan MA Xiaofang GUO Xuezhen
		Objective To explore the status and influencing factors of ICU nurses' knowledge, attitudes and practices (KAP) about sedation in Shiyan. Methods This study was a convenience study, from January to June 2016, a total of 147 ICU nurses from Shiyan Taihe Hospital, Shiyan People's Hospital and Dongfeng General Hospital were interviewed. The measurement instrument included self-designed basic information questionnaire about ICU nurses and ICU nurses' KAP questionnaire. Results ICU nurses' sedation nursing KAP total score was (62.08±10.42) points, the score of each dimension were (10.79±2.05), (27.74±3.51), (23.37±4.36) points. Education, length of ICU working, professional ranks of nurses and ICU specialized nurses or not were the main factors influencing the level of ICU nurses' KAP (P < 0.05). Conclusion ICU nurses' KAP are in the middle levels, which are influenced by education, length of ICU working, professional ranks and ICU specialized nurses or not. ICU managements should be combined with the weak link of different nurses to carry out targeted training to improve clinical quality of sedation.
		2017 Vol. 14 (18): 53-55,63 [Abstract] ( 167 ) HTML (1 KB) PDF (516 KB) ( 70 )

	56	Analysis on basic health status of family in Heilongjiang Province
		JIA Yuehui QI Yanbo HAN Yunfeng XIE Zhiping GE Jie▲
		Objective To explore the basic health status of residents in Heilongjiang Province, in order to provide data for the relevant departments formulating health policies. Methods The method of stratified-cluster random sampling was used to obtain samples. A total of 6601 households which contained 18 016 residents were investigated in 2013 in Heilongjiang Province. By the training of qualified investigators to conduct surveys and collect questionnaires. SAS 9.3 software was used for statistical analysis, and the data were compared with the fourth health service survey in 2008. Results In Heilongjiang Province, the housing type in urban areas were mainly about building and brick house. The housing type in rural areas were mainly about brick and adobe house, the housing type had improved, but the proportion of adobe housing was still up to 28.1% in rural areas. Compared with the results in 2008 (37.5% of adobe housing), the living conditions were still poor. The proportion of tap water for residents in urban and rural areas were 96.7% and 56.2%. The proportion of sanitary latrines were 92.5% and 69.6%. The proportions of tap water and sanitary latrines in rural areas were significantly lower than those of cities, which had tough task. Disease and injury was the primary cause of poverty, followed by less labor force. The medical institutions in the urban areas were mainly general hospitals, clinics/village clinics and community health service centers. Rural areas were mainly in clinics or village clinics, accountted for 88.4%. Conclusion The medical conditions in rural areas are poor, the of living conditions, tasks of tap water and sanitary latrines are difficulty. Regardless of urban or rural, disease and injury are the primary cause of poverty.
		2017 Vol. 14 (18): 56-58 [Abstract] ( 147 ) HTML (1 KB) PDF (555 KB) ( 35 )

	59	A survey of professional cognition and learning effect of general practice in postgraduates of general practice major
		FAN Hui JIA Yumei ZHANG Pengrui WANG Guang▲
		Objective To investigate and compare the understand and learning effect of general family medicine (GFM) in master postgraduates major in general practice (GP) and other directions. Methods Through the questionnaire survey, this study compared the understanding and identification of GFM, study method and effects and the willingness serve in a community health center in master postgraduates of GP (40 cases) and other majors (40 cases) of Capital Medical University from September 2014 to July 2016. Results GP master postgraduates were more interesting in chronic disease management than other majors, so did the understanding and identification of GFM (P < 0.05). GP master postgraduates thought learning GEM would be benefit for future occupation. The willingness to serve in a community health center and to engage in chronic disease management was stronger in GP master postgraduates than other majors, although there were concerns about the working environment, income and academic status, professional development, but this ratios in GP master postgraduates were significantly lower than other professional graduate students (P < 0.05). A case-report based learning and practice in community hospitals was well accepted by all of the master postgraduates, and they expected more international communications (P > 0.05). Conclusion Postgraduate education for GP provides sufficient professional knowledge and clinical understanding of GFM, which are helpful for master postgraduates in employment preparation and chronic disease management. So postgraduate education for GP should be further supported.
		2017 Vol. 14 (18): 59-63 [Abstract] ( 172 ) HTML (1 KB) PDF (512 KB) ( 87 )

	64	Study on the standardization of diagnostic name of outpatient diseases
		LI Jingnan1 DUAN Qingchuan2 ZHAO Jinshun1 LIU Jing1 CHEN Jianming1 XIA Zhiwei1▲
		Objective To analyze the type of non-standard diagnosis writing of outpatient diseases, and to explore the effective measures for physicians' writing standards. Methods A retrospective study was conducted on the outpatient disease diagnosis writing in Peking University Third Hospital from September 2007 to March 2014. Results There were 4804 non-standard outpatient diagnosis writing. The types of non-standard diagnosis could be divided into abbreviated disease name (137 items), disease name written by English (99 items), non-standardized medical terms (2021 items), reversed the order of diagnostic name (24 items), undefined or too detailed diagnosis (1143 items), and written by non-classified anatomical site in ICD-10 (1380 items). Conclusion In view of the irregular type of diagnosis writing of outpatient diseases, the clinicians should be trained to standardize the clinical thinking habits, improve the classification of diseases and promote the support of health information development.
		2017 Vol. 14 (18): 64-67 [Abstract] ( 231 ) HTML (1 KB) PDF (571 KB) ( 103 )

	68	Predictive value of plasma baseline thyrotropin levels in nonthyroid illness syndrome patients with mechanical ventilation
		LIU Ping1 ZANG Xuefeng1 ZHEN Jie1 ZHU Xi2 CHEN Wei1▲
		Objective To analyze predictive value of plasma baseline thyrotropin levels in nonthyroid illness syndrome (NTIS) patients with mechanical ventilation during hospitalization. Methods From January 2015 to August 2016, 188 patients received incubation and had no history of thyroid illness in ICU of Beijing Shijitan Hospital were continuously enrolled. All patients were divided into NTIS group of 156 cases and without NTIS group of 32 cases according to thyroid function. The morbidity and clinical characters of NTIS patients were summarized. 156 patients with NTIS were divided into two subgroups of low T3 syndrome and low T4 syndrome according to T3 and T4 levels. 156 patients with NTIS were divided into three subgroups of ＜0.27 mIU/L, 0.27-＜2.50 mIU/L, 2.50-＜4.50 mIU/L according to TSH level. Clinical data and prognosis of each group were compared. Results The morbidity of NTIS was 83%. The levels of PCT and CRP in NTIS group were higher than those in without NTIS group, while the levels of ALB and Ca2+ were lower than those in without NTIS group (P < 0.05). The levels of ALB, Ca2+ and Hb in low T4 syndrome group were lower than those in low T3 syndrome group (P < 0.05). TSH 2.50-<4.50 mIU/L group had the lowest T3 level, TSH 0.27-<2.50 mIU/L group had the shortest mechanical ventilation time (P < 0.05). COX survival analysis showed that TSH 2.50-<4.50 mIU/L was an independent predictor of good prognosis for 14 days (P ＜ 0.05). There was no significant difference in prognosis between different levels of TSH group (P > 0.05). Conclusion For patients with mechanical ventilation with NTIS, TSH 2.50-<4.50 mIU/L is an independent predictor of good prognosis for 14 days.
		2017 Vol. 14 (18): 68-71,75 [Abstract] ( 167 ) HTML (1 KB) PDF (442 KB) ( 103 )

	72	Changes of taste sensibility in patients with type 2 diabetes mellitus
		ZHANG Lu1 BI Huimin2▲ GAO Ling3
		Objective To explore sensitivity changes of sweetness, salty and sour taste in patients with type 2 diabetes mellitus. Methods Endocrinology outpatient and inpatient patients with type 2 diabetes mellitus and outpatient physical examination of healthy people from March 2015 to September 2016 in People's Hospital of Wuhan University were selected as study subjects. According to WHO (1999) diagnostic criteria for diabetes, the subjects were divided into type 2 diabetes mellitus group and control group, 20 patients in each group. The sex, age, height, weight, abdominal circumference, fasting blood glucose, glycosylated hemoglobin, history of diabetes, history of drug use, history of alcohol and so on of subjects were collected. So that subjects were tasting the concentration from low to high preparation of the sweet (sucrose), salty (sodium chloride), sour agent (citric acid). The concentrations were divided into different digital levels in response to the lowest concentration levels that can be recorded. The resulting data was processed by using the statistical software SPSS 20.0. The changes of taste (sweet, salty and acid) sensitivities in patients with type 2 diabetes mellitus and its relationship with the relevant measurement indicators were analyzed. Results The sweetness sensitivity of type 2 diabetes mellitus group was lower than that of control group (4.700±1.337 vs 6.500±1.179, P < 0.01). The sour sensitivity of type 2 diabetes mellitus group was lower than that of control group (6.200±0.919 vs 5.100±0.994, P < 0.05). There was no significant difference in salty susceptibility between type 2 diabetes mellitus group and control group (4.900±1.101 vs 5.400±0.843，P > 0.05). Fasting blood glucose levels were negatively correlated with sweetness (r = －0.622, P < 0.01). Abdominal circumference, body mass index, alcohol and tobacco history had no significant correlation with taste sensitivity (P > 0.05). Conclusion The sensitivity of sweetness in patients with type 2 diabetes is lower than that in healthy people, and the sensitivity of acidity is higher than that of healthy people. Fasting blood glucose and the sensitivity of sweetness is negatively correlated. Abdominal circumference, body mass index, whether smoking and drinking and taste sensitivity have nothing to do.
		2017 Vol. 14 (18): 72-75 [Abstract] ( 154 ) HTML (1 KB) PDF (446 KB) ( 77 )

	76	Effect of micro percutaneous nephrolithotomy combined with high power holmium laser in the treatment of complex nephritic stone
		YU Rutong1 JIANG Quan1 ZHANG Jian1 CHEN Zhiqiang1 CUI Yujie2 SUN Guoliang1
		Objective To investigate the efficacy and safety of micro percutaneous nephrolithotomy combined with high power holmium laser in the treatment of complex nephritic stone. Methods 60 patients with complex nephritic stone in Chengde Central Hospital, Hebei Province from March 2015 to September 2016 were selected as research subjects. They were randomly divided into observation group (n=30) and control group (n=30) by random number table, observation group was treated with micro percutaneous nephrolithotomy combined with high power holmium laser, control group was treated with percutaneous nephrolithotomy, the clinical indexs (intraoperative blood loss, operation time, hospitalization time and postoperative time to get out of bed, stone clearance, recurrence and complications, the levels of serum creatinine (Cr) and urea nitrogen (BUN) at preoperative and postoperative 2 h, 24 h, 7 d, 30 d between two groups were compared. Results The intraoperative blood loss in observation group was less than that in control group, time of operation, the time of hospitalization and the time of getting out of bed in observation group were significantly shorter than those in control group, the differences were statistically significant (P < 0.05). The removal rate of stone in observation group (93.3%) was significantly higher than that in control group (73.3%), the recurrence rate in observation group (3.3%) was significantly lower than that in control group (20.0%), the differences were statistically significant (P < 0.05). The incidence of postoperative complications in observation group (6.6%) was significantly lower than that in control group (36.7%), the differences was statistically significant (P < 0.05). The levels of serum Cr and BUN at postoperative 24 h, 7 d, 30 d in observation group were significantly lower than those in control group, the differences were statistically significant (P < 0.05). Conclusion Micro-renal mirror combined with high-power holmium laser in the treatment of complex kidney stones is good, safe, contribute to renal function recovery. It is worthy of clinical promotion and application.
		2017 Vol. 14 (18): 76-79 [Abstract] ( 197 ) HTML (1 KB) PDF (388 KB) ( 98 )

	80	Clinical effect of percutaneous endoscopic lumbar discectomy for lumbar disc herniation
		FU Zuchang1 WANG Qingkeng1 YOU Ruijin1▲ ZHOU Panyu2
		Objective To observe the clinical effect of percutaneous endoscopic lumbar discectomy (PELD) in the treatment of patients with lumbar disc herniation. Methods From January 2013 to January 2016, in 180 Hospital of PLA, 126 patients with lumbar disc herniation were divided into two groups according to the random number table, with 63 cases in each group. The observation group was given PELD, the control group was given fenestration discectomy, they were followed up for 12 months. The operative incision length, operation time, hemorrhage volume and hospitalization days of two groups were compared. The clinical outcome were evaluated by visual analogue score (VAS), Oswestry disability index (ODI) and modified MacNab standard. Results Operative incision length, hemorrhage volume and hospitalization days in observation group was significantly less than control group, the differences were statistically significant (P < 0.05). The ODI and VAS of two groups were improved after operation, and the improvement in observation group was significantly more obvious than control group, the differences were statistically significant (P < 0.05). The curative effect evaluated modified MacNab standard of observation group was better than control group, the difference was statistically significant (P < 0.05). Conclusion The application of PELD in the treatment of lumbar disc herniation can reduce hemorrhage volume, decrease operative incision length, shorten operation time, improve treatment efficacy and improve patients, quality of life. It is worth of clinical promotion.
		2017 Vol. 14 (18): 80-83 [Abstract] ( 395 ) HTML (1 KB) PDF (601 KB) ( 100 )

	84	long-term effect observation of neck roadside block screw fixation in the treatment of lower cervical vertebrae fracture dislocation
		JIA Yongpeng LIN Jie SUN Junjian JIA Jinlong WANG Chaoyang▲
		Objective To observe the long-term efficacy of neck roadside block screw fixation in the treatment of lower cervical vertebrae fracture dislocation. Methods 90 cases of patients with lower cervical vertebrae fracture dislocation treated in the 98th Hospital of PLA from December 2014 to December 2016 were selected. All the patients were divided into the control group and the observation group according to the different operation types, 45 cases in each group. Patients in the control group were given anterior cervical plate fixation treatment, while patients in the observation group were given neck roadside block screw fixation. The operation time, intraoperative blood loss, fracture healing time, postoperative complications, the spinal cord function score and neural function grading after 12 months of follow-up were compared between the two groups. Results Compared with the control group, the observation group had shorter operation time, less intraoperative blood loss, with statistically significant differences (P < 0.05). There were no significant differences in fracture healing time and postoperative complication rate between the two groups (P > 0.05). The JOA scores of the two groups increased after treatment compared with before treatment, with statistically significant differences (P < 0.05). 12 months after treatment JOA score of the two groups had no significant difference (P > 0.05). The neurologic ASIA grading of two groups after treatment dramatically improve compared with before treatment, and the improvement of observation group were more remarkable after treatment than the control group after treatment, with statistically significant difference (P < 0.05). Conclusion The long-term efficacy of neck roadside block screw fixation in the treatment of lower cervical vertebrae fracture dislocation is better than the anterior cervical plate fixation, but it need strictly comply with the clinical indications to ensure the satisfied clinical efficacy.
		2017 Vol. 14 (18): 84-87,90 [Abstract] ( 154 ) HTML (1 KB) PDF (591 KB) ( 114 )

	87	Change of interleukin-17 levels in both synovial fluid and plasm of patients with primary knee osteoarthritis accompanied by meniscus injuries after arthroscopic surgery
		ZHANG Haisen1 BAI Yuming1 LIU Chang1 JIN Shengli1 SU Ke1 LIU Ying2 LYU Zhichang3
		Objective To investigate interleukin-17 (IL-17) levels in both synovial fluid and plasm of patients with primary knee medial osteoarthritis (OA) accompanied by medial meniscus injuries after arthroscopic surgery. Methods 46 patients with primary knee OA accompanied by medial meniscus injuries underwent arthroscopic surgery in Central Hospital of Cangzhou from January 2014 to June 2015 were selected as the experimental group, and 30 healthy individuals were selected as the control group. Visual analog scale (VAS) pain score and Lysholm score were used to evaluate the pain level and function of the knees of patients with OA. The IL-17 concentrations in both plasma and synovial fluid of patients were measured at preoperative period and postoperative 3, 6, 12 months, and in plasma of controls were determined using auantitative sandwich enzyme-linked immunoassay. Results Postoperative incisions were Ⅰ stage healing for patients. All patients were followed up for 12-18 months, average of 15 months. Postoperative VAS pain scores significantly decreased and Lysholm scores significantly increased compared with preoperative periods (P < 0.05). For VAS scores and Lysholm scores, there were no statistically significant differences between postoperative points (P > 0.05). Patients before arthroscopic surgery had higher plasma IL-17 concentrations compared to control group (P < 0.05). Average postoperative IL-17 levels in plasma at each time point of patients were significant lower than preoperative periods (P < 0.05), but higher than control group (P < 0.05). Postoperative plasm and synovial fluid IL-17 levels of patients after the surgery had no statistically significant differences among postoperative points (P > 0.05). Conclusion Arthroscopic surgery can significantly improve the pain symptoms and joint function and reduce IL-17 levels in both synovial fluid and plasm of the patients with knee OA accompanied by medial meniscus injuries, but IL-17 can not back to healthy level.
		2017 Vol. 14 (18): 87-90 [Abstract] ( 162 ) HTML (1 KB) PDF (580 KB) ( 79 )

	91	Effect on hip replacement in treatment of intertrochanteric fracture with severe comminuted displacement in elderly patients
		LI Guijian LIU Peijuan LUO Jiawu SHEN Xuehao
		Objective To the effect of hip replacement in elderly patients with severe intertrochanteric fractures with severe comminuted displacement. Methods From June 2014 to June 2016, in Department of Orthopedics, Nanxiong People's Hospital, 94 elderly patients (≥60 yeras old) with intertrochanteric fractures were selected, and they were divided into two groups according to different surgical methods, 45 patients underwent internal fixation were as control group, 49 patients underwent hip replacement were as test group. The prognosis (fracture healing time, postoperative joint activity time) of the two groups of patients were compared, the overall curative effect was evaluated by Harris score, the postoperative complications of the two groups during the follow-up 3 months were observed and recorded. Results After treatment, the fractures of the two groups were healed. In the test group, the duration of fracture healing was (32.4±9.2) d, the time of postoperative joint movement was (7.3±1.6) d, after 2 months Harris quantitative score of hip function was (93.6±2.0) scores, those in the control group were (42.7±10.6) d, (18.5±2.7) d, (82.6±3.4) scores respectively, the differences were statistically significant (P < 0.05).The total effective rates of the test group and the control group were 95.9% and 75.6%, the clinical curative effect of the test group was significantly better than that of the control group, the difference was statistically significant (P < 0.05). The incidence of postoperative complications in the control group and the test group were 17.78% and 6.12%, the difference was statistically significant (P < 0.05). Conclusion After the hip replacement, middle-aged and elderly patients with severe intertrochanteric fractures with severe comminuted displacement are better healed in fracture and their healing cycle is shorter with higher safety, thus effectively improving the quality of life of patients.
		2017 Vol. 14 (18): 91-93,105 [Abstract] ( 125 ) HTML (1 KB) PDF (590 KB) ( 109 )

	94	Posterior interbody fusion and intervertebral foramen fusion therapy comparison of clinical curative effect of degenerative lumbar spondylolisthesis with vertebral fracture
		HOU Gang1 WANG Siqing2▲ SUN Dafeng2 LU Xiao1 ZHOU Jinjun1
		Objective To investigate the clinical effect of posterior interbody fusion and intervertebral foramen fusion in the treatment of degenerative lumbar spondylolisthesis with vertebral fracture. Methods From January 2011 to December 2015, in Department of Orthopedics, Dongtai People's Hospital, the clinical data of 120 patients with vertebral fracture degeneration lumbar spondy lolisthesis were analyzed retrospectively. according to the operation method they were divided into observation group and control group, 60 cases with posterior interbody fusion were as control group, and 60 cases with intervertebral foremen fusion were as observation group. The operative time, hospitalization cost and intraoperative blood loss of the two groups were compared. 1 year after operation, the JOA scores were compared, the X-ray films were regularly reviewed to observe the fusion and lumbar stability. Results The operation time, intraoperative blood loss and hospitalization cost were in the observation group lower than those in the control group, the differences were statistically significant (P < 0.05). The ODI of the observation group was lower than that of the control group, the difference was statistically significant (P <0.05). There was no significant difference of fusion rate between the two groups (P > 0.05). There was no significant difference in vertebral activity and translucent band width between the two groups (P > 0.05), but the average height of the intervertebral space of control group was lower than that of the observation group, the difference was statistically significant (P <0.05). In the control group, there were 2 cases of cerebrospinal fluid leakage, which was related to the separation of scar adhesions during operation. During the follow-up, there was no dislocation of the fusion, sinking and nail bar loosening, and the fracture occurred. No infection in both groups. Conclusion Intervertebral forumen fusion is superior to lumbar spondylolisthesis in patients with lumbar spondylolisthesis. The clinical effect is satisfactory, the stability of lumbar vertebra is good and the fusion rate is high. It is worthy of clinical application.
		2017 Vol. 14 (18): 94-97 [Abstract] ( 142 ) HTML (1 KB) PDF (704 KB) ( 122 )

	98	Changes of perioperative blood sedimentation and serum inflammatory cytokines concentrations in patients with femoral neck fracture and artificial femoral head replacement treatment and its clinical significance
		WANG Chuangming ZHENG Xun CHEN Haibo ZENG Qingqiang
		Objective To study the changes of perioperative blood sedimentation and serum inflammatory cytokines concentrations in patients with femoral neck fracture and artificial femoral head replacement treatment and its clinical significance. Methods From May 2013 to May 2015, in Traditional Chinese Medical Hospital of Shantou City, 66 patients with femoral neck fracture and artificial femoral head replacement treatment were selected, they were given postoperative 1 year follow-up. Perioperative blood sedimentation and serum inflammatory cytokines concentrations of the were detected. Results Perioperative blood sedimentation and serum IL-6, TNF-α and PCT and other inflammatory markers levels in patients with infection were higher than that of patients without infection, the differences were statistically significant (P < 0.05). After 1 year follow-up, perioperative blood sedimentation and inflammatory markers levels in patients with Harris score ≥80 points were lower than that of patients with Harris score <80 points, the differences were statistically significant (P < 0.05). Perioperative blood sedimentation and serum IL-6, TNF-α and PCT levels in patients with femoral neck fracture and artificial femoral head replacement treatment were correlated with the infection incidence and Harris scores (P < 0.05). ROC curve analysis results showed that value of perioperative blood sedimentation and serum inflammatory factors levels in patient with femoral neck fracture and had artificial femoral head replacement treatment unitedly diagnosing the infection was high; and the value of perioperative blood sedimentation and serum inflammatory factors levels in patient with femoral neck fracture and had artificial femoral head replacement treatment unitedly predicting the hip function recovery was also good. Conclusion Perioperative blood sedimentation and serum inflammatory factors levels in patient with femoral neck fracture and had artificial femoral head replacement treatment are closely related to the infection and hip function, and it may help to evaluating the infection and the hip joint function recovery.
		2017 Vol. 14 (18): 98-101 [Abstract] ( 137 ) HTML (1 KB) PDF (674 KB) ( 41 )

	102	Application value of fibula support ankle arthrodesis in Kashin-Beck disease talus necrosis
		XIE Xiaomin ZHOU Xiaoqing JIA Bin GOU Bo▲
		Objective To study the effect of fibula support ankle arthrodesis in the treatment of Kashin-Beck disease talus necrosis (KTN). Methods A total of 45 cases with KTN treated in Department of Orthopaedics, Baoji Hospital of Traditional Chinese Medicine from January 2012 to January 2016 were selected. All patients were given fibula support ankle arthrodesis treatment. The follow-up conditons were recorded. X ray of the anklelateral was taken to observe the bone fusion, and given histological observation. Bone mineral density (BMD), the maximum compression strength and the American association of ankle surgery (AOFAS) score were compared before surgery and 6, 12 months after surgery. Results All patients were followed-up after surgery, with the average time of (8.5±1.1) months. The incisions were removed the stitches in 2 weeks after surgery. X ray showed bone callus formation in 4 weeks after surgery. Pain was relieved in 5 weeks after surgery, foot shape had improvement. BMD test and the corresponding biomechanics test results showed that, the BMD and the maximum compression strength had increased, the differences were statistically significant (P < 0.05). The differences in different time of AOFAS scores were statistically significant (P < 0.05). Conclusion Fibula support ankle arthrodesis in the treatment of KTN has fast healing and fusion, can achieve complete osseous healing, and fewer complications, postoperative recovery of BMD and biomechanics function are good, pain has relief, comprehensive clinical effect is good. It is worthy of popularization and widely application.
		2017 Vol. 14 (18): 102-105 [Abstract] ( 177 ) HTML (1 KB) PDF (717 KB) ( 78 )

	106	Analysis of maternal and neonatal outcomes of different chorionic twins
		LI Ting FENG Bibo▲ ZHAI Jianjun
		Objective To analyze maternal and neonatal outcomes of different chorionic twins, in order to investigate the effect of chorionic villi on the complications of pregnant women. Methods The clinical data of 559 twin pregnancy women in Beijing Tongren Hospital from January 2011 to December 2015 were collected, according to the results of chorionic nature by ultrasound in early trimester and pathologic checking the placenta and membrane after delivery, they were divided into two groups: monochorionic diamniotic twin pregnancy group (monochorionic twin group, 198 cases) and diachorionic diamniotic twin pregnancy group (diachorionic twin group, 361 cases), the pregnancy complications of pregnant women and outcomes of fetal-neonate of two groups were compared. Results The incidence of complications during pregnancy such as gestational hypertension，gestational diabetes, polyhydramnios, premature rupture of membranes and placenta abrupture and sevene postpartum hemorrhage of monochorionic twin group was 24.7%, 16.7%, 14.6%, 18.2%, 4.6%, 4.0% respectively, those of the diachorionic twin group was 21.6%, 15.5%, 13.6%, 17.5%, 3.9%,4.7% respectively, there was a statistically significant only in the incidence of preterm of two groups (P < 0.05). The fetal loss, complicated twin, asphyxia rate of neonatal and mortality rate of the monochorionic twin group (17.7%, 54.6%, 13.1%, 4.6% respectively) were significantly higher than those of the diachorionic twin group (4.4%, 21.3%, 4.4%, 2.8% respectively), the difference were statistically significant (P < 0.05). Conclusion Chorionic properties of pregnancy complications in two groups have no difference, but monochorionic twin pregnancies has a higher risk, it should be accurately determine about the chorionic nature in the first trimester, setting up monitoring and intervention measures during pregnancy to improve the outcomes of fetal-newborn.
		2017 Vol. 14 (18): 106-108 [Abstract] ( 142 ) HTML (1 KB) PDF (457 KB) ( 92 )

	109	Value of high risk HPV combined liquid-based cytology testing in screening cervical disease in pregnancy women
		NI Qian ZHOU Yuehua NI Yunxiang▲
		Objective To investigate the necessity of routine screening of cervical diseases during pregnancy through the high-risk HPV detection combined with TCT examination. Methods From January 2013 to December 2015, in outpatinet of Obstetrics, Shanghai Tongren Hospital, 7316 pregnant women received the high-risk HPV detection and TCT examination in the first prenatal examination were selected. Pregnant women with high-risk HPV positive or abnormal TCT results received the colposcopy assessment, and those abnormal women further received the microscopic histological biopsy. Results In 7316 pregnant women, there were 584 high-risk HPV positive pregnant women, accounting for 7.98%, and 180 pregnant women with abnormal TCT examination, accounting for 2.46%. The sensitivity, specificity, positive predictive value and negative predictive value of high-risk HPV detection for cervical lesions was 91.40%, 93.09%, 14.55% and 99.88%, respectively, those of TCT examination was 86.02%, 98.62%, 44.44% and 99.82%, respectively, and those of high-risk HPV detection combined with TCT examination was 100.00%, 92.55%, 14.74% and 100.00%, respectively. There was no significant correlation between HPV load and severity of cervical lesions (P > 0.05). Conclusion In terms of cervical disease screening for pregnant women, the high-risk HPV detection combined with TCT examination has a higher sensitivity and negative predictive value than that of single application of HPV detection and TCT examination, which can find the cervical lesions timely, so that the disease can be detected and treated early.
		2017 Vol. 14 (18): 109-112 [Abstract] ( 170 ) HTML (1 KB) PDF (509 KB) ( 35 )

	113	Efficacy of three approaches for hysterectomy in patients with uterine myoma
		LIU Yusheng ZHANG Ying DUAN Hua GUO Yinshu▲
		Objective To compare the efficacy of three operative approaches (laparotomy, laparoscopy, vaginal) for hysterectomy in patients with uterine myoma. Methods From September 2009 to January 2015, in Beijing Obstetricsand Gynecology Hospital, Capital Medical University, the clinical data of 725 patients received hysterectomy for uterine myoma were retrospectively analyzed, they were divided into three groups: trans-abdominal hysterectomy group (TAH group, 283 cases), trans-laparoscopic hysterectomy group (TLH, 251 cases) and trans-vaginal hysterectomy group (TVH, 191 cases). The operative time, intraoperative bleeding, postoperative exhaust time, eating time, body temperature, complications, hospitalization time and costs of three groups were compared. Results Three groups were compared, intraoperative bleeding of TAH group was the highest, operative time was the longest, hospitalization costs was the highest, postoperative highest body temperature, exhaust time, eating time, postoperative hospitalization time and total hospitalization time were the lowest, the differences were statistically significant (P < 0.05). The complication rates of three groups were compared, the differences were not statistically significant (P > 0.05). Conclusion TLH is superior to TVH and TAH in terms of intraoperative bleeding, postoperative recovery, hospitalization time and total hospitalization time, except of a longer operative time and higher hospitalization costs. All three hysterectomy approaches have their own advantages and disadvantages, suitable surgical procedure should be chosen according to different condition.
		2017 Vol. 14 (18): 113-116,120 [Abstract] ( 148 ) HTML (1 KB) PDF (531 KB) ( 84 )

	117	Research of ovarian reserve by counting the number of antral follicle count, anti Mullerian hormone and sex hormone
		HAN Xiaoxia ZUO Ruiju MA Xiao
		Objective To compare the difference between antral follicle count (AFC), anti Mullerian hormone (AMH) and sex hormone in predicting ovarian function. Methods From January 2014 to December 2015, in Reproductive Center, Cangzhou People's Hospital, 284 female infertility patients received in vitro fertilization and embryo transfer (IVF-ET) treatment were selected as the research object, according to the number of oocytes, they were divided into groups of poor ovarian response group (193 cases) and normal ovarian response group (91 cases). Before treatment by transvaginal ultrasound, the diameter 3-10 mm AFC number were calculated, on the third day of menstrual blood test AMH and basal hormone levels (FSH, LH, E2, FSH/LH ratio), the sensitivity and specificity of AFC, AMH and sex hormone in the evaluation of ovarian reserve function were analyzed. Results AMH and AFC of poor ovarian response group were less than the normal ovarian response group, FSH and E2 value of poor ovarian response group was higher than the normal ovarian response group, FSH/LH value of poor ovarian response group was more than the normal ovarian response group, the differences were statistically significant (P < 0.05). The sensitivity and specificity of AMH projecting poor ovarian response were 0.91 and 0.72, the sensitivity and specificity of AFC were 0.88 and 0.93, the sensitivity and specificity of FSH/LH were 0.78 and 0.72, these were higher than the levels of single basal sex hormones. Conclusion The sensitivity and specificity of AFC, AMH and FSH/LH predicting ovarian reserve function are stronger than those of single hormone FSH, LH and E2.
		2017 Vol. 14 (18): 117-120 [Abstract] ( 132 ) HTML (1 KB) PDF (510 KB) ( 90 )

	121	Clinical study of the visual quality after implantation of an aspherical intraocular lens ZCB00
		LIAN Huifang1 MA Weisong2 AN Lin1 CHEN Chen1 WEI Qiuhong1▲
		Objective To compare the impact on functional vision of the aspheric Tecnis ZCB00 lens with the spherical Sensar AR40e lens. Methods From January to July in 2015, 60 patients with cataract in the First Central Hospital of Baoding were selected and divided into observation group and control group according to random number table, with 30 cases in each group. All cataract patients underwent phacoemulsification were randomized to receive an aspheric IOL (AMO Tecnis ZCB00 , as observation group) or a spherical IOL (AMO AR40e, as control group). Uncorrected visual acuity, best corrected visual acuity (BCVA), contrast sensitivity function (CSF), spherical aberration of the whole eye and internal optics (IOL) at 5 mm pupil diameter and modulation transfer function（MTF) were examined 3 months after postoperatively. Results The postoperative visual activity was not significantly different between the two groups (P > 0.05).The postoperative high order aberration of the eye at 5 mm pupil diameter in the observation group was less than that in the control group (P ＜ 0.01). In the bulk of spatial frequencies, including photopic vision (6, 12, 18 cpd), photopic vision with glare conditions (except 1.5 cpd), scotopic vision (1.5, 6, 12 cpd) and scotopic vision with glare, mean CSF generally in the observation group was higher than that in the control group, with statistical significance (P < 0.05). The values of MTF in the observation group were higher than those in the control group except 30 cpd in 5.0 mm pupil sizes (P ＜ 0.05). Conclusion Comparing to the conventional spherical IOL, the negative aspheric IOL may reduce the spherical aberration and improve visual performance of pseudophakic eyes.
		2017 Vol. 14 (18): 121-124,129 [Abstract] ( 383 ) HTML (1 KB) PDF (554 KB) ( 130 )

	125	Study on the effect of adrenaline on gingiva based on the gingival biotype
		SONG Yuanying1 ZHAO Qiang2▲
		Objective To study the effect on gingival after treating with retraction cord of adrenaline for 5 minutes based on the gingival biotype. Methods From June to December in 2015, 15 internships with thick type and 15 internships with thin type from Beijing Aviation General Hospital were selected as volunteers, their bilateral maxillary central incisors were selected as experimental objects. Gingival retraction was performed by placing retraction cords with or without adrenaline on left and right maxillary central incisor respectively. The distance from incisal to free gingival margin of each sample was measured 3 times by vernier caliper before gingival retraction and 5 minutes, 1 day, 3 days, 7 days and 14 days after gingival retraction. The average value of the data measured above was taken as the final data. Results ①There was no significant difference in the distance from incisal to free gingival margin between 3 days after gingival retraction and before gingival retraction when treated with retraction cord of non-drug in thin type (P > 0.05). ②There was no significant difference in the distance from incisal to free gingival margin between 7 days after gingival retraction and before gingival retraction when treated with retraction cord of adrenaline in thin type (P > 0.05). There was no significant difference in the distance from incisal to free gingival margin between 3 days after gingival retraction and before gingival retraction when treated with retraction cord of adrenaline in thicktype (P > 0.05). ③After treated with retraction cord of adrenaline, the ratio of gingival recession distance to the distance from incisal to free gingival margin of thin typewere significantly changed compared with thick type at 5 mintues, 1 day and 3 days (P < 0.05). Conclusion ①It takes longer time for the gingival margin to recover the previous height in thin type when treats with retraction cord of adrenaline for 5 minutes compares with retraction cord of non-drug. ②No matter thick or thin type, the treatment with retraction cord of adrenaline for 5 mintues will not cause permanent damage to the gingiva. ③The gingival recession distance of thin type is longer than thick type when treats with retraction cord of adrenaline for 5 minutes.
		2017 Vol. 14 (18): 125-129 [Abstract] ( 152 ) HTML (1 KB) PDF (609 KB) ( 121 )

	130	Effect of anterior apical osteotomy on occlusal relationship in patients with bimaxillary protrusion
		XIE Fang XU Jiajie LU Jianjian ZHANG Chao YANG Liya LI Shuyuan TENG Li▲
		Objective To study effect of anterior apical osteotomy on occlusal relationship in patients with bimaxillary protrusion. Methods From September 2003 to October 2014, 20 patients with bimaxillary protrusion were included in this study, who underwent anterior subapical osteotomy of both the maxilla and mandible in Plastic Surgery Hospital, Chinese Academy of Medical Sciences. In addition, the pre- and postoperative cephalometric radiographs were systematically analyzed. Results After surgery, the levels of U1-OP, L1-OP, IMPA, U1E-Apog, L1E-Apog, U1E-NA, L1-NB, L1E-NB were decreased than before surgery, and the levels of ⅡA and U1-NA were increased than before surgery, with high statistically significant difference (P < 0.01). Conclusion With the use of a precise preoperative model surgical design, orthognathic surgery, a simple and time-saving technique, can be used to correct bimaxillary protrusion with satisfactory postoperative occlusal relationship, and minimal postoperative complications.
		2017 Vol. 14 (18): 130-133 [Abstract] ( 124 ) HTML (1 KB) PDF (703 KB) ( 46 )

	134	The effect observation of high-dose Methylprednisolone treatment on Vogt-Koyanagi-Harada disease
		ZHU Maoli HUANG Liang XUE Min DONG Jianhong▲
		Objective To observe the effect of high-dose Methylprednisolone treatment on Vogt-Koyanagi-Harada disease. Methods Sixty-nine hospitalized patients (135 eyes) diagnosed with Vogt-Koyanagi-Harada disease from January 2013 to January 2016 in Xuhui District Central Hospital of Shanghai were analyzed retrospectively. All patients received high-dose Methylprednisolone treatment, with Methylprednisolone 1.0 g/d in early mornings for three consecutive days, and then the dosage was tapered based on the condition improvement. Before and after being treated for three and seven days, all patients underwent vision acuity examination, fundus examination, optical coherence tomography (OCT) and fundus fluorescein angiography (FFA). The changes were analyzed, and the patients were followed up for one year. Results After treatment for one week, there were 100 eyes (74.1%) whose vision acuity were improved significantly [best corrected vision acuity (BCVA)≥0.6], retina edema subsided, retinal detachment disappeared in OCT and fluorescence leakage reduced in FFA. All of the 32 eyes (23.7%) with vision acuity slowly improved were treated after an onset of no less than two weeks. The BCVA was improved slowly after treatment for two weeks. There were 3 eyes (2.2%) that showed no response to the high-dose methylprednisolone treatment and then received immunosuppressant therapy. Analysis of logMAR vision converted from BCVA, vision acuity of all patients was improved obviously after treatment for three days and seven days compared to before treatment, with statistically significant differences (P ＜ 0.01); moreover, vision acuity of all patients was improved markedly after treatment for seven days compared to that after treatment for three days, and the difference was of statistical significance (P ＜ 0.01). Analysis of OCT: compared to conditions before treatment, foveal thickness (ILM-RPE), macular retinal volume and average volume thickness were decreased greatly after treatment for three days and seven days, and the difference was of statistical significance (P ＜ 0.01). Compared to results after treatment for three days, macular foveal thickness (ILM-RPE), macular retinal volume and average volume thickness were decreased greatly after treatment for seven days, and the difference was of statistical significance (P ＜ 0.01). During the one-year follow-up, no serious corticosteroid complications occurred to all eyes. Three patients (6 eyes) had a relapse within six months after treatment, and were given high-dose Methylprednisolone treatment again, which ended well. Conclusion High-dose Methylprednisolone treatment can effectively control the Vogt-Koyanagi-Harada disease, with fast visual function recovery, low recurrence rate and rare adverse reaction. It is safe and effective. The earlier the treatment is given, the better the curative effect will be.
		2017 Vol. 14 (18): 134-137 [Abstract] ( 171 ) HTML (1 KB) PDF (680 KB) ( 109 )

	138	Clinical effects of Gelan Xinning Soft Capsules in the treatment of angina pectoris in coronary heart disease and the influence on lipid metabolism
		RUAN Xiaofen WANG Yingjie SHAO Chunlin GAO Junjie CHEN Tiejun XIA Nan WANG Xiaolong
		Objective To observe the clinical effects of Gelan Xinning Soft Capsules in the treatment of angina pectoris in coronary heart disease and the influence on lipid metabolism. Methods One hundred and twenty patients with angina pectoris in coronary heart disease hospitalized in Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Xinhua Hospital Affiliated to Shanghai Jiao Tong University, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, Putuo Central Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from October 2015 to September 2016 were selected and randomly divided into control group (40 cases) and Gelan Xinning group (80 cases) by the ratio of 1∶2. Both groups were treated with conventional western medicine, on basis of which, Gelan Xinning group was treated with Gelan Xinning Soft Capsules, the course was 12 weeks. The efficacy of TCM syndromes, scores of angina pectoris, changes of blood lipid in the two groups were observed. Results The total effective rate of Gelan Xinning group was 78.6%, which was higher than that in the control group (29.4%), the difference was highly statistically significant (P ＜ 0.01). After treatment, the scores of angina pectoris in the two groups were all significantly lower than those before treatment (P ＜ 0.01), and the scores of angina pectoris in the Gelan Xinning group was significantly lower than that in the control group (P ＜ 0.01). After treatment, the levels of total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) in the two groups were all statistically significantly lower than those before treatment (P ＜ 0.05 or P ＜ 0.01), the levels of triglyceride (TG), non-high-density lipoprotein cholesterol (non-HDL-C) in the Gelan Xinning group were significantly lower than those before treatment (P ＜ 0.01), while there were no statistically significant differences of TG, non-HDL-C within the control group before and after treatment (P ＞ 0.05). There were no statistically significant differences of high density lipoprotein cholesterol (HDL-C), apolipoprotein A (ApoA), apolipoprotein B (ApoB) within the two groups before and after treatment (P ＞ 0.05). After treatment, the TG in Gelan Xinning group was lower than that of control group, ApoA was higher than that of control group, the differences were all statistically significant (P ＜ 0.05). Conclusion On basis of conventional treatment, Gelan Xinning Soft Capsules in the treatment of patients with angina pectoris in coronary heart disease can significantly improve the clinical symptoms and regulate the lipid metabolism, and it is safe and effective.
		2017 Vol. 14 (18): 138-141,152 [Abstract] ( 162 ) HTML (1 KB) PDF (555 KB) ( 81 )

	142	Clinical study of Zhikang Granules in the treatment of nonalcoholic fatty liver disease
		ZHAO Xiumin LI Fengxue GU Jing
		Objective To observe the clinical curative effect of Zhikang Granules in the treatment of nonalcoholic fatty liver disease. Methods Eighty patients with nonalcoholic fatty liver disease admitted to Department of Integrated Chinese and Western Medicines, the Second Hospital of Hebei Medical University from October 2014 to October 2015 were randomly divided into the treatment group (42 cases) and the control group (38 cases). The control group was treated with Atorvastatin orally, while the treatment group was treated with Atorvastatin and Zhikang Granules orally. The course of the two groups was 12 weeks. Results The total effective rate of treatment group and control group was 92.86%, 55.26% respectively, the difference was statistically significant (P ＜ 0.05). After treatment, the levels of triacylglycerol, total cholesterol, glutamic oxalacetic transaminase, glutamic-pyruvic transaminase in the two groups were all lower than those before treatment (P ＜ 0.05), and the levels of triacylglycerol, total cholesterol, glutamic oxalacetic transaminase, glutamic-pyruvic transaminase in the treatment group after treatment were lower than those of control group, the differences were statistically significant (P ＜ 0.05). After treatment, the levels of insulin resistance index, fasting blood-glucose in the two groups were all lower than those before treatment (P ＜ 0.05), and the levels of insulin resistance index, fasting blood-glucose in the treatment group after treatment were lower than those of control group, the differences were statistically significant (P ＜ 0.05). After treatment, the subjective uncomfortable symptoms of giddiness and tinnitus, fullness in the head, chest distress in the two groups were significantly improved compared with those before treatment (P ＜ 0.05), and the improvement of treatment group was significantly better than that of control group (P ＜ 0.05). Conclusion The curative effect of Zhikang Granules in the treatment of nonalcoholic fatty liver disease is distinct, which is worthy of clinical promotion and application.
		2017 Vol. 14 (18): 142-144 [Abstract] ( 166 ) HTML (1 KB) PDF (498 KB) ( 40 )

	145	Clinical efficacy of Butylphthalide in combination with Alprostadil Injection in the treatment of posterior circulation ischemic vertigo
		LIU Yafang1,2 HUANG Yongmei3▲ DAI Jun1 CAO Xiongbin1,2 SHI Yunqiong1
		Objective To observe the curative efficacy of Butylphthalide in combination with Alprostadil Injection in patients with posterior circulation ischemic vertigo. Methods One hundred and ten patients with posterior circulation ischemic vertigo who received therapy in Huangshi Central Hospital of Edong Healthcare Group from January to October 2016 were collected as observational objects and randomly divided into the control group (55 cases) and the observation group (55 cases) according to random number table. Besides conventional treatment, the control group was treated with Alprostadil Injection, while the observation group was treated with Butylphthalide in combination with Alprostadil Injection. After treatment of 2 weeks, the curative efficacy, blood velocity of posterior circulation before and after treatment and adverse reactions were compared. Results Assessment of curative efficacy of vertigo showed that the total effective rate in the observation group was statistically higher than that in the control group (94.5% vs 81.8%, P ＜ 0.05). Before treatment, there were no significant differences of blood velocity of basilar artery, left vertebral artery and right vertebral artery between the two groups (P ＞ 0.05). After treatment, the indexes above in the two groups were all significantly faster than those before treatment (all P ＜ 0.05), and blood velocity of basilar artery, left vertebral artery and right vertebral artery in the observation group was higher than those of control group (all P ＜ 0.05). During the treatment, there was no case with severe adverse reactions, and the incidence of adverse reactions in the control group and the observation group had no statistically significant difference (5.4% vs 7.3%, P ＞ 0.05). Conclusion Butylphthalide in combination with Alprostadil Injection is effective for patients with posterior circulation ischemic vertigo, which can improve the curative efficacy, speed up the blood velocity of posterior circulation, with low incidence of adverse reactions, it is worthy of clinical application.
		2017 Vol. 14 (18): 145-148 [Abstract] ( 203 ) HTML (1 KB) PDF (506 KB) ( 65 )

	149	Effect of Shenqi Fuzheng Injection combined with Simvastatin in the treatment of sepsis and its influence on vascular endothelial function
		XUE Yulin1 WANG Yujuan2
		Objective To investigate the effect of Shenqi Fuzheng Injection combined with Simvastatin in the treatment of sepsis and its influence on vascular endothelial function. Methods The clinical data of 92 patients with sepsis admitted to Xinjiang Cardiovascular Hospital from January to December 2016 was analyzed retrospectively, and the patients were divided into control group (46 cases) and observation group (46 cases) according to different treating methods. On basis of conventional treatment, the control group was given Simvastatin, while the observation group was treated with adjuvant therapy of Shenqi Fuzheng Injection based on the treatment of control group. The clinical efficacy, the scores of acute physiology and chronic health evaluation scoring system (APACHEⅡ) and sequential organ failure assessment (SOFA), and the vascular endothelial function of the two groups were compared. Results After treatment, the total effective rate of observation group was statistically significantly higher than that of the control group (89.1% vs 71.7%, P ＜ 0.05). After treatment, the scores of APACHEⅡ and SOFA in the two groups were all lower than those before treatment (all P ＜ 0.05), and the scores of APACHEⅡ and SOFA in the observation group after treatment were all lower than those of the control group, the differences were all statistically significant (all P ＜ 0.05). The indexes of vascular endothelial function showed that the levels of endothelin-1 (ET-1) after treatment in the two groups were all lower than those before treatment, the levels of nitric oxide (NO) after treatment in the two groups were all higher than those before treatment (all P ＜ 0.05), and the levels of ET-1 after treatment in the observation group were lower than those of control group, the levels of NO after treatment in the observation group were higher than those of control group (all P ＜ 0.05). Conclusion Application of Shenqi Fuzheng Injection combined with Simvastatin in sepsis has good effects, which can increase the clinical efficacy, relieve the clinical symptoms and improve the vascular endothelial function, it is worthy of clinical application.
		2017 Vol. 14 (18): 149-152 [Abstract] ( 163 ) HTML (1 KB) PDF (509 KB) ( 48 )

	153	A case of acute myocardial pericarditis after radiofrequency ablation of atrial fibrillation
		HAN Yujuan1 DING Ligang2 ZHENG Lihui2 CHEN Gang2 LV Chunmei1
		Acute myocarditis involving pericarditis is rare in clinical practice, because of its diagnosis is based on endomyocardial biopsy and pathological examination. Clinical application is limited, grassroots hospitals more difficult to do. Clinically often encountered a high degree of suspected acute myocardial pericarditis, because of its clinical manifestations and acute myocardial infarction similar to the auxiliary examination, such as continuous not relieve chest pain, increases myocardial enzymes and electrocardiogram ST segment elevation, clinical misdiagnosis rate is high. so in such patients should ask for details of the history, analysis of the illness, improve the level of clinical diagnosis and increase diagnosis rate.
		2017 Vol. 14 (18): 153-156,164 [Abstract] ( 155 ) HTML (1 KB) PDF (1609 KB) ( 106 )

	157	Literature research on TCM medication law of HBV-related hepatocirrhosis
		YANG Xianzhao1,2 JIANG Feng2,3 RU Shuying2,4 ZHANG Peng2,3 LI Ying2,3 ZHANG Ludan2,3 YE Yong′an2,3
		Objective To analyze the traditional Chinese medicine (TCM) medication law of HBV-related hepatocirrhosis based on the past clinical research literatures. Methods The clinical research literatures on TCM for HBV-related hepatocirrhosis from inception to September 2016 was searched in PubMed, Chinese biomedical literature database (CBM), China National Knowledge Infrastructure (CNKI), WanFang Data, VIP network. A database of TCM decoctions for HBV-related hepatocirrhosis was established. The frequency of medication was analyzed. The effect, nature and flavor of herbs were descriptively analyzed. Results A total of 118 articles were included. There were 121 prescriptions, 204 herbs and 1356 herb frequency involved. There were 11 herbs with a frequency greater than 30. The top 5 were Radix Astragali, Radix Salviae Miltiorrhizae, Carapax Trionycis, Rhizoma Atractylodis Macrocephalae and Radix Bupleuri. The 204 herbs could be divided into 19 categories according to the effect. The top 5 were deficiency-supplementing herb, blood-quickening stasis-transforming herb, heat-clearing herb, dampness-removing herb and qi-regulating herb, with a total frequency of 80.23%. In the frequency of herb nature, the top 3 were cold, warm and calm. The total frequency of cold herbs and slightly cold herbs was 37.75%. In the frequency of herb flavor, the top 3 were bitter, sweet and pungent. The frequency of bitter herbs was 32.40%. Conclusion The general rule of TCM in the treatment of HBV-related hepatocirrhosis is strengthening the body resistance and eliminating pathogenic factors. In the specific principle, on the basis of qi-boosting spleen-supplementing yin-nourishing therapy, blood-quickening stasis-transforming therapy, heat-clearing toxin-resolving therapy, dampness-clearing therapy and liver-coursing qi-rectifying therapy are often used in treating HBV-related hepatocirrhosis.
		2017 Vol. 14 (18): 157-160,180 [Abstract] ( 183 ) HTML (1 KB) PDF (833 KB) ( 80 )

	161	Relationship between the expression quantity of miR-21 and the degree of atherosclerosis and the influence of Vinpocetine on miR-21
		WANG Shan YANG Huayu YAO Lan WANG Yunchao CHEN Haiping MA Qing▲
		Objective To study the correlation between different degrees of atherosclerosis and the expression quantity of miR-21, and the influence of Vinpocetine application on the expression quantity of miR-21. Methods Forty patients with atherosclerosis hospitalized in Medical Care Center, Beijing Friendship Hospital, Capital Medical University from January 2014 to December 2016 were selected, and they were divided into mild (9 cases), moderate (6 cases) and severe (25 cases) according to the degree of atherosclerosis, and they were also divided into medication group (17 cases) and control group (23 cases) according to the treatment method. The serum RNA of patients was extracted and the levels of serum miR-21 in the patients with different degrees of atherosclerosis were analyzed by real-time quantitative PCR, the changes of miR-21 and haematocrit before and after treatment in the medication group and control group were compared. Results The expression of miR-21 in patients with severe atherosclerosis was higher than that in patients with mild atherosclerosis, the difference was statistically significant (P ＜ 0.05). After treatment, the haematocrit and relative contents of miR-21 in the medication group were lower than those before treatment, the differences were statistically significant (P ＜ 0.05), there was no statistically significant difference of the relative contents of miR-21 after treatment between medication group and control group (P ＞ 0.05). Conclusion The levels of serum miR-21 in patients with atherosclerosis are increased with the increase of atherosclerosis, and the expression of miR-21 is significantly decreased after treatment with Vinpocetine, which indicates that miR-21 has correlation with atherosclerosis.
		2017 Vol. 14 (18): 161-164 [Abstract] ( 164 ) HTML (1 KB) PDF (781 KB) ( 102 )

	165	Discussion on the military-civilian combination first aid mode of trauma in major traffic accidents
		FENG Xikun1,2 LIU Wei1▲ ZHOU Caixing1 WANG Xin′gang1
		This article focused on the practical requirement for trauma care organization after fatal traffic accidents. Firstly, the complexity of serious traffic accident care and the necessity of military-civilian combination rescue are analyzed. Then, difficulties in military-civilian combination rescue are systematically analyzed, including difficulties in legislation, institution, technology, and so on. And on this basis, the paper puts forward an idea about constructing military-civilian combination first aid mode of trauma in major traffic accidents, and systematically formulates from construction principle, hardware construction, and professional training. It will be a good guidance and reference for the future traffic accident rescue.
		2017 Vol. 14 (18): 165-167 [Abstract] ( 186 ) HTML (1 KB) PDF (758 KB) ( 42 )

	168	Analysis on the development trend and characteristics of Yunnan traditional Chinese medicine health industry
		HAN Xinxin XU Yingjian ZHANG Difan GAO Jie
		Yunnan traditional Chinese medicine health industry has a profound historical heritage. Further accelerate the development has a solid foundation. However, the existence of resource advantages failed to form an industrial advantage and it lacks of a large number of research personnel and a series of constraints. The next step of Yunnan traditional Chinese medicine health industry key development is to promote the development of industrial integration, to create "Yunyao" name card, promote the combination of education and medicine, pad short board of the human resources of Chinese medicine, promote the inheritance and innovation of science and technology and lead tourism upgrade.
		2017 Vol. 14 (18): 168-171 [Abstract] ( 181 ) HTML (1 KB) PDF (769 KB) ( 27 )

	172	Analysis on the social psychological adaptation status and its influence fatctors of patients with intestinal stoma
		HUANG Meixue HUANG Yanqing LING Yufang WEI Suyu GU Yali
		Objective To study the social psychological adaptation status and its influencing factors of patients with intestinal stoma, in order to provide preconditions for the improvement of these patients′social psychological adaptation. Methods 100 patients with intestinal stoma in Affiliated Hospital of Youjiang Medical College for Nationalities from March 2014 to May 2016 were selected as the study object, and they were evaluated by OAI-20 scale . Then make the patients′OAI-20 scale assessment results were made separate comparison according to personal basic condition (gender, ages, education degree, living condition and economic condition), intestinal stoma related situation (intestinal stoma complications, intestinal stoma time, cceptance degree of intestinal stoma, self-care ability of intestinal stoma and recognition for intestinal stoma knowledge) and other situation(social support degree, emotional state, general self-efficacy and modes of medical care provider payment), and the relationship between the above mentioned factors and the social psychological adaptation status of the patients with intestinal stoma was analyzed by Logistic regression analysis. Results The OAI-20 scale results of 100 patients with intestinal stoma were bad, and the OAI-20 scale results of patients with different gender, ages, education degree, living condition, economic condition, intestinal stoma complications, intestinal stoma time, cceptance degree of intestinal stoma, self-care ability of intestinal stoma, recognition for intestinal stoma knowledge, social support degree, emotional state, general self-efficacy and ways of medical expenses had significant differences (P ＜ 0.05). The Logistic regression analysis showed those factors were all the important influencing factors for the social psychological adaptation status of the patients with intestinal stoma (P ＜ 0.05). Conclusion The social psychological adaptation status of patients with intestinal stoma are relatively bad, and the personal basic condition, intestinal stoma related situation and other situation are the important influencing factors, so they should be intervened regarding to these factors.
		2017 Vol. 14 (18): 172-176 [Abstract] ( 174 ) HTML (1 KB) PDF (734 KB) ( 58 )

	177	Effect of intellectual activity on cognitive function of retired elderly
		WEI Xiaoli1,2 WANG Suoliang3▲
		Objective To discuss the effect of intellectual activity on cognitive function of retired elderly. Methods The cross-sectional study and longitudinal follow-up study were undertaken among 188 physical examination cases in Baoji Center Hospital from October 2014 to October 2015. The intellectual activity group (94 cases of retired elderly, 60 years old and above) often took part in any one or more of the following intellectual activities for 5 years and above, including playing chess, go, mahjong, cards, checkers, calligraphy, painting, reading, etc. The frequency of such intellectual activities was more than twice per week and 1-3 hours once. According to gender, age and educational level, another 94 cases of retired elderly were screened out as control group, who never take part in the intellectual activities mentioned above or just attend occasionally. Such patients suffered from cognitive impairment were eliminated. The cognitive function of each case was evaluated by such scales as self-made questionnaire, Hamilton depression scale (HAMD), Mini mental state evaluation (MMSE), Montreal Cognitive Assessment (MoCA) and activities of daily living (ADL). When each case received health check-up one year later, the scales mentioned above were used to evaluate the cognitive function again. The score changes were compared between the two groups mentioned above. Results There were no significant differences between two groups in HAMD scores, MMSE scores, MoCA scores and ADL scores when enrolling (P > 0.05). There was still no significant differences between two groups in HAMD scores, MMSE scores and ADL scores (P > 0.05), while the MoCA scores in the intellectual activity group were significantly higher than that of the controlled group (P < 0.05). The MoCA analysis showed the scores of 3 cases (3.2%) below the critical value of cognitive impairment in the intellectual activity group, reaching the standard of the mild cognitive impairment, significantly less than that in the control group, which was 7 cases (7.4%) (P < 0.05). Conclusion The intellectual activities may be advantageous to the prevention of cognitive decline in retired elderly, and the MoCA scale may be more suitable than others for screening MCI.
		2017 Vol. 14 (18): 177-180 [Abstract] ( 144 ) HTML (1 KB) PDF (774 KB) ( 80 )

	181	The effectiveness of nursing practice reform in Department of Chest Surgery
		KANG Li1 HAO Nan2▲
		Objective To establish the specific contents of nursing parctice reform based on core competency in Department of Chest Surgery. Methods 41 nursing students were selected as control group, who were in Department of Chest Surgery, the First Affiliated Hospital of Xi′an Jiaotong University (for short "our hospital") from May 2015 to January 2016. And 43 nursing students were selected as experimental group, who were in our hospital Department of Chest Surgery from May 2016 to January 2017. The nursing practice model based on core competency was used in experimental group and original nursing practice method was used in control group. Three indicators were used to evaluate the reform effectiveness, including the comprehensive ability, teachers′ satisfaction with students and students′ satisfaction with teachers. Results The scores of basic knowledge and nursing skills in the experimental group were higher than those in the control group (P < 0.05). The scores of teachers′ satisfaction with students in the experimental group were higher than those in the control group (P < 0.05), except about nursing malpractice (P > 0.05). The students in experimental group were more satisfied with teachers (P < 0.05). Conclusion The nursing practice reform based on core competency can play a positive role in the teachers and nursing students.
		2017 Vol. 14 (18): 181-183 [Abstract] ( 146 ) HTML (1 KB) PDF (761 KB) ( 47 )

	184	Cultivation and management of clinical scientific research compound talents in hospital from the perspective of translational mMedicine
		FU Hui1,2 LU Xiang2
		With the concept of translational medicine into Chinese medical and health system and development planning, large-scale general hospital urgent need to train a number of clinical scientific research compound talents topromote the development of translational medicine and promote the transformation of scientific research. This study describes the concept and development of translational medicine, defines the concept of clinical scientific research compound talents and analyzes its characteristics, explores the training methods and management of clinical scientific research compound talents in large-scale general hospitals, and summarizes 6 aspects, including: hospital managers attaching great importance, clinicians changing their minds, building an academic platform to provide a good scientific environment, perfecting the incentive mechanism and establishing the standard of personnel evaluation, teamwork "project education" and paying attention to post-graduate education. Combined with the innovation of personnel system and scientific research system of the Second Affiliated Hospital of Nanjing Medical University, the construction of multi-dimensional evaluation system of high-level compound talents in hospital, and the talent cultivation and scientific research achievements in the past 5 years, analyze the existing problems and put forward reasonable suggestions and thinking for reference.
		2017 Vol. 14 (18): 184-187 [Abstract] ( 177 ) HTML (1 KB) PDF (731 KB) ( 53 )

	188	Research for adjust TNF-α of medical collagen for the treatment of allergic contact dermatitis mechanism
		SUN Weijun1△ WANG Fuqiang2△ XU Wenlong1 WANG Zhang3 SONG Wenjie3 WANG Jie3 LIU Guangli1 LIU Jingsong3 CHEN Yilong1
		Objective To study the adjust TNF-α of medical collagen treats ACD and mechanism. Methods Duplication ACD models were made, the mouse skin was smeared with this medical collagen for about 14 days as a therapy, ear tissue were given histopathological examination, ELISA was used to test the levels of TNF-α,IL-2 inflammatory mediators. Results Compared with pre attack, ear allergy were very obvious in all group after attack 12, 24 h (P < 0.01). Compared with blank control group, the ear allergy increased in medical collagen low does group after attack 12 h (P < 0.05), the ear allergy increased in model control group after attack 24 h (P < 0.05). Compared with model control group, the ear allergy reduced in medical collagen low, middle, high does group after attack 24h (P < 0.05). Compared with blank control group, inflammatory cells of the right ear increased in all group mouse (P < 0.05). Compared with model control group, inflammatory cells of the right ear reduced in dexamethasone control group, medical collagen low, high does group (P < 0.05 or P < 0.01). Compared with blank control group, the left and right ear piece thickness difference enlarged obviously in model control group, dexamethasone control group, medical collagen middle and high does group (P < 0.01). Compared with model control group, the left and right ear piece thickness difference decreased in medical collagen low does group mouse (P < 0.05). Compared with blank control group, TNF-α level increased obviously in model control group and medical collagen low does group mouse (P < 0.05). Compared with model control group, TNF-α's level reduced in dexamethasone control group and medical collagen high does group mouse (P < 0.01 or P < 0.05). Meanwhile, this therapy did not influenced the animals' weight. Conclusion Medical collagen can be a certain medical treatment for ACD, but it may connect with the adjust of the level of TNF-α and then decrease of the ear inflammation.
		2017 Vol. 14 (18): 188-192 [Abstract] ( 190 ) HTML (1 KB) PDF (1159 KB) ( 52 )

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