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| Relationship between the expression quantity of miR-21 and the degree of atherosclerosis and the influence of Vinpocetine on miR-21 |
| WANG Shan YANG Huayu YAO Lan WANG Yunchao CHEN Haiping MA Qing▲ |
| Medical Care Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China |
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Abstract Objective To study the correlation between different degrees of atherosclerosis and the expression quantity of miR-21, and the influence of Vinpocetine application on the expression quantity of miR-21. Methods Forty patients with atherosclerosis hospitalized in Medical Care Center, Beijing Friendship Hospital, Capital Medical University from January 2014 to December 2016 were selected, and they were divided into mild (9 cases), moderate (6 cases) and severe (25 cases) according to the degree of atherosclerosis, and they were also divided into medication group (17 cases) and control group (23 cases) according to the treatment method. The serum RNA of patients was extracted and the levels of serum miR-21 in the patients with different degrees of atherosclerosis were analyzed by real-time quantitative PCR, the changes of miR-21 and haematocrit before and after treatment in the medication group and control group were compared. Results The expression of miR-21 in patients with severe atherosclerosis was higher than that in patients with mild atherosclerosis, the difference was statistically significant (P ＜ 0.05). After treatment, the haematocrit and relative contents of miR-21 in the medication group were lower than those before treatment, the differences were statistically significant (P ＜ 0.05), there was no statistically significant difference of the relative contents of miR-21 after treatment between medication group and control group (P ＞ 0.05). Conclusion The levels of serum miR-21 in patients with atherosclerosis are increased with the increase of atherosclerosis, and the expression of miR-21 is significantly decreased after treatment with Vinpocetine, which indicates that miR-21 has correlation with atherosclerosis.
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