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| Peimine inhibits cell proliferation in human leukemia cell line K562 through redox imbalance |
| QI Yan LIAO Bin XU Chengbo HUANGFU Zhenping CHEN Jiawei |
| Department of Hematology, People′s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fujian Province, Fuzhou 350004, China |
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Abstract Objective To investigate the effect of peimine on cell proliferation of human leukemia K562 cells and the function of redox imbalance in this progress. Methods The human leukemia K562 cells were cultured in vitro. After K562 cells were treated with peimine at concentrations of 50, 100, 200, 400 and 800 μmol/L for 24 h, the cell proliferation was measured by thiazolyl blue tetrazolium bromide (MTT) assay. And the half maximal inhibitory concentration (IC50) of peimine was calculated using the Bliss method. The changes of intracellular reactive oxygen species (ROS) and glutathione (GSH) concentration were detected using spectrophotometry. Results Peimine inhibited the proliferation of K562 cells in a dose-dependent manner. And the IC50 of peimine was 238 μmol/L. Furthermore, peimine could promote the ROS production and glutathione (GSH) depletion. The balance of oxidation-antioxidation function destroyed. Pre-incubation with antioxidants GSH or N acetyl cysteine (NAC) almost abolished the effects of peimine. Conclusion The present study therefore shows that peimine inhibits the proliferation activity in K562 cells. The redox imbalance may play a key role in this process.
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[1] 卓诗勤，张浩，丁弋娜，等.硫熏和鲜切浙贝母的化学成分及其药理作用的比较研究[J].中华中医药学刊，2016， 34（3）：618-621.
[2] 黄长煌.蒸发光散射测定浙贝母中贝母素甲、贝母素乙的含量探究[J].中国卫生标准管理，2016，7（18）：139-141.
[3] 谌海燕，陈信义.贝母素甲抑制人乳腺癌细胞-增殖及其对细胞凋亡的影响[J].中医药学报，2012，40（4）：12-15.
[4] 王冰，宋锐，牟宗玲，等.中药逆转肿瘤多药耐药的相关研究[J].中医学报，2016，31（7）：946-948.
[5] 刘韦鋆，邹富胜，李东华.浙贝母抑制耐药肿瘤P糖蛋白的活性组分研究[J].中国中西医结合外科杂志，2015，22（4）：379-382.
[6] 唐晓勇，唐迎雪.浙贝母碱对肺癌细胞多药耐药的逆转作用观察及机制探讨[J].山东医药，2012，52（18）：4-6.
[7] 胡凯文，郑洪霞，齐静，等.浙贝母碱逆转白血病细胞多药耐药的研究[J].中华血液学杂志，1999，20（12）：33-34.
[8] 李冬云，陈信义，姜靖雯.复方浙贝颗粒研究现状与应用前景分析[J].中国药物与临床，2009，9（2）：85-87.
[9] 陈俊宇，李俊玮，梁源，等.活性氧：肿瘤进展的双刃剑[J].中国实验诊断学，2016，20（9）：1598-1600.
[10] Song W，Hu P，Shan Y，et al. Cartilage polysaccharide induces apoptosis in K562 cells through a reac，ve oxygen species-mediated caspase pathway [J]. Food Funct，2014， 5（10）：2486-2493.
[11] 段坦然，孟春春，唐兆新，等.活性氧在肿瘤发展和治疗中的作用[J].中国细胞生物学学报，2016，38（10）：1295-1301.
[12] 杨梦祺，刘盼盼，黄蓬.肿瘤氧化还原代谢与干预[J].中国生化药物杂志，2016，36（9）：16-23.
[13] Landry WD，Woolley JF，Cotter TG. Imatinib and Nilotinib inhibit Bcr-Abl-induced ROS through targeted degradation of the NADPH oxidase subunit p22phox [J]. Leuk Res，2013，37（2）：183-189.
[14] Khoshtabiat L，Mahdavi M，Dehghan G，et al. Oxidative stress-induced apoptosis in chronic myelogenous leukemia K562 cells by an active compound from the dithio-carbamate family [J]. Asian Pacific J Cancer Prev，2016，17（9）：4267-4273.
[15] 王海娟，张美芝，刘小菊，等.雷公藤内酯醇逆转肿瘤多药耐药研究进展[J].中国实验方剂学杂志，2016，22（10）：229-234.
[16] Ak?觭akaya H，Tok S，Dal F，et al. β-carotene treatment alters the cellular death process in oxidative stress-induced K562 cells [J].Cell Biol Int，2017，41（3）：309-319.
[17] Yao JY，Wei X，Lu YH.Chaetominine reduces MRP1-mediated drug resistance via inhibiting PI3KAktNrf2 signaling pathway in K562Adr human leukemia cells [J]. Biochem Bioph Res Co，2016，473（4）：867-873.
[18] Xiao R，Cui WX，Yang L，et al. Extraction of total alkaloids，peimine and peiminine from the flower of Fritillaria thunbergii Miq using supercritical carbon dioxide [J]. J CO2 Utiliz，2017，18（2）：283-293.
[19] Zhang J，Cao M，Yang WW，et al. Inhibition of glucose-6-phosphate dehydrogenase could enhance 1，4-benzoquinone-induced oxidative damage in K562 cells [J]. Oxid Med Cell Longev，2016，2016（21）：1-11.
[20] 郑智，侯丽，许亚梅，等.复方浙贝颗粒联合阿霉素影响K562/A02移植瘤细胞耐药相关酶表达研究[J].医学研究杂志，2009，38（12）：29-31，146. |
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