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| Research for adjust TNF-α of medical collagen for the treatment of allergic contact dermatitis mechanism |
| SUN Weijun1△ WANG Fuqiang2△ XU Wenlong1 WANG Zhang3 SONG Wenjie3 WANG Jie3 LIU Guangli1 LIU Jingsong3 CHEN Yilong1 |
1.College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Sichuan Province, Chengdu 611137, China;
2.Meishan Food and Drug Control Center, Sichuan Province, Meishan 620010, China;
3.College of Ethnomedicine, Chengdu University of Traditional Chinese Medicine, Sichuan Province, Chengdu 611137, China |
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Abstract Objective To study the adjust TNF-α of medical collagen treats ACD and mechanism. Methods Duplication ACD models were made, the mouse skin was smeared with this medical collagen for about 14 days as a therapy, ear tissue were given histopathological examination, ELISA was used to test the levels of TNF-α,IL-2 inflammatory mediators. Results Compared with pre attack, ear allergy were very obvious in all group after attack 12, 24 h (P < 0.01). Compared with blank control group, the ear allergy increased in medical collagen low does group after attack 12 h (P < 0.05), the ear allergy increased in model control group after attack 24 h (P < 0.05). Compared with model control group, the ear allergy reduced in medical collagen low, middle, high does group after attack 24h (P < 0.05). Compared with blank control group, inflammatory cells of the right ear increased in all group mouse (P < 0.05). Compared with model control group, inflammatory cells of the right ear reduced in dexamethasone control group, medical collagen low, high does group (P < 0.05 or P < 0.01). Compared with blank control group, the left and right ear piece thickness difference enlarged obviously in model control group, dexamethasone control group, medical collagen middle and high does group (P < 0.01). Compared with model control group, the left and right ear piece thickness difference decreased in medical collagen low does group mouse (P < 0.05). Compared with blank control group, TNF-α level increased obviously in model control group and medical collagen low does group mouse (P < 0.05). Compared with model control group, TNF-α's level reduced in dexamethasone control group and medical collagen high does group mouse (P < 0.01 or P < 0.05). Meanwhile, this therapy did not influenced the animals' weight. Conclusion Medical collagen can be a certain medical treatment for ACD, but it may connect with the adjust of the level of TNF-α and then decrease of the ear inflammation.
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