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2019 Vol. 16, No. 31
Published: 2019-11-05
 
 
4 Effect and mechanism of oleanolic acid on proliferation and apoptosis of lung adenocarcinoma A549 cells
LI Kairui1 LIU Jie1 LI Xiaona1 HE Yingchun2,3,4 XU Zhaojun5,6 SONG Lan2,3,4
Objective To investigate the effects of oleanolic acid on proliferation and apoptosis of lung adenocarcinoma A549 cells, and to explore its mechanism. Methods A549 cells were divided into control group (DMSO), different concentrations of oleanolic acid group (0.625, 1.25, 2.5, 5, 10 μmol/L) and positive control group (Cisplatin, 0.004 g/L) according to different culture methods. MTT and real-time cellular analysis technology (RTCA) were used to detect cell proliferation. Hoechst 33342 staining, mitochondrial membrane potential test, Annexin V-FITC/PI double fluorescent staining were used to detect cell apoptosis. Western blot was used to detect protein expression. Results MTT assay showed that the proliferation rate of A549 cells treated with oleanolic acid at different concentrations for 24, 48 and 72 hours was lower than that of the control group (P < 0.01). The results of RTCA showed that oleanolic acid could inhibit the proliferation of A549 cells. The results of Hoechst 33342 staining showed that compared with the control group, the fluorescence intensity of each group increased significantly (P < 0.05 or P < 0.01). The results of JC-1 staining showed that compared with control group, the red fluorescence intensity decreased significantly and the green fluorescence intensity increased significantly (P < 0.05 or P < 0.01). Annexin V-FITC/PI double fluorescent staining results showed that compared with the control group, the apoptosis of the treated groups increased significantly (P < 0.05 or P < 0.01). Oleanolic acid can down regulate the expression of anti apoptotic protein XIAP, survivin, Bcl-2, up regulate the expression of apoptotic protein Bax, cleaved casepase 3, cleaved caspase 8. Compared with control group, the difference was statistically significant (P < 0.05 or P < 0.01). Conclusion Oleanolic acid can inhibit the proliferation and induce apoptosis of lung adenocarcinoma cells, and its induction of apoptosis may be through the death receptor pathway or the mitochondrial pathway.
2019 Vol. 16 (31): 4-9 [Abstract] ( 209 ) HTML (1 KB)  PDF (1323 KB)  ( 81 )
10 Study of mitochondrial protein quality control in gastric smooth muscles of rats in spleen qi deficiency
XU Xinzhu1 LI Zhenyu2 LIU Wenjun3 WANG Yixuan1 CHEN Wenna4 SONG Nan5 SHAN Dehong6
Objective To explore the possible mechanism of stomach losing dropping in deficiency of spleen qi around mitochondrial protein quality control (MPQC) in gastric smooth muscles. Methods A total of 16 male SD rats were randomly divided into normal group and deficiency of spleen qi group (model group), with 8 rats in each group. The gastric residual rate was detected by charcoal powder propelling method, the ATP level in the gastric antrum smooth muscles(GASMs) was measured by ELISA, the mitochondrial structures in GASMs was observed by transmission electron microscopy, the expressions of hot shock protein 70 (Hsp70), Lon protease (LonP), caseinolytic protease (Clp)XP complex in GASMs were investigated by Western blot. Results Compared with the normal group, the gastric residual rate was greater (P ＜ 0.01), the ATP level was lower (P ＜ 0.05), the number of mitochondria was declined and some mitochondria became vacuolar, the expression of Hsp70 was unaltered, the expressions of LonP, ClpX and ClpP were all increased (P ＜ 0.01) in the model group. Conclusion The stomach losing dropping deficiency of spleen qi may be related to the decrease of mitochondrial productivity caused by MPQC abnormality of gastric antrum.
2019 Vol. 16 (31): 10-13 [Abstract] ( 219 ) HTML (1 KB)  PDF (648 KB)  ( 129 )
14 Effect of OPG-HSP70 on serological parameters of CIA mouse model and its correlation with efficacy
XIA Sisi1,2 ZHAO Wenming2 RONG Yujia2
Objective To investigate the relationship between serum parameters and disease status in collagen-induced arthritis (CIA) mice after OPG-HSP70 treatment. Methods Forty-four male DBA/1 mice were divided into OPG-HSP70 treatment group (n = 20), OPG treatment group (n = 12) and model control group (n = 12). CIA model was induced by intradermal immunization of bovine CⅡ in the tail root. OPG or OPG-HSP70 fusion protein was injected intraperitoneally every other day after arthritis, whereas model control group was injected with the same amount of PBS solution. The mice were sacrificed after 4 weeks of administration, the arthritis index was evaluated, and the relative gray value of bone was measured by Micro-CT. The serum was collected to detect Ca2+, RANKL and type Ⅱ collagen (CⅡ) antibodies. The linear regression was analysis between arthritis index and bone density values and serum data. Results Compared with the model control group, arthritis index of OPG-HSP70 treatment group was significantly reduced (P ＜ 0.01), the BMD value increased obviously (P ＜ 0.05), the CⅡ antibodies lower significantly (P ＜ 0.01). However, OPG did not significantly improve inflammation and bone damage. Linear regression analysis showed that, the serum Ca2+ and RANKL were negatively correlated with the bone density value (r = -0.582, -0.588, all P ＜ 0.01); while the serum CⅡ antibody level was positively correlated with the arthritis index (r = 0.892, P ＜ 0.01). Compared with the OPG treatment group, serum anti-OPG antibodies were significantly lower in the OPG-HSP70 treatment group 1, 2, 3, 4 weeks after administration (P ＜ 0.01). Conclusion The OPG-HSP70 fusion protein adds anti-inflammatory effect and reduces immunogenicity, and the protection of CIA mice is better than that of OPG.
2019 Vol. 16 (31): 14-17 [Abstract] ( 209 ) HTML (1 KB)  PDF (617 KB)  ( 79 )
18 Expression and significance of IL-17 and NF-κB in the rats with acute gout arthritis
SHEN Ruiming ZHONG Liangbao ZHENG Yanping LIANG Haiqin
Objective To investigate the expression and significance of interleukin-17 (IL-17) and nuclear factor kappa-B (NF-κB) in the acute gouty arthritis (AGA) rat model. Methods A total of 45 SD rats were divided into control group (9 rats) and model group (including four subgroups: 6, 12, 24 h and 72 h, 9 rats in each group) by random number table. A rat model of AGA was induced with the injection of monosodium urate (MSU) into the right posterior ankle joint cavity. The rats of the control group were injected with an equal dose of saline. The model group was sacrificed at 6, 12, 24 h and 72 h, respectively. The expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-17 (IL-17) in serum and synovium was detected by the enzyme-linked immunosorbent assay. The expression of NF-κB in synovial membrane was detected by Western blot. Results Compared with the control group, the expression of IL-1β, TNF-α and IL-17 in the model group was significantly increased at 6 h after AGA (P < 0.01), peaked at 12 h, and began to decrease at 24 h. The relative expression level of p-NF-κB p65 in the synovial membrane of the model group was significantly higher than that of the control group, and gradually decreased with time. Conclusion After AGA, the expression levels of IL-17 and p-NF-κB p65 are significantly increased, and the inflammatory response is aggravated. The differential expression of IL-17 and NF-κB is closely related to the occurrence and development of AGA.
2019 Vol. 16 (31): 18-21 [Abstract] ( 226 ) HTML (1 KB)  PDF (668 KB)  ( 45 )
22 Pharmacokinetic interaction of Biapenem with Methotrexate and Furosemide in rats
DONG Jing1 LI Tingting2 QIU Zhixia3 LU Yan1 LI Wenyan1
Objective To investigate the pharmacokinetic interaction of Biapenem with Methotrexate and Furosemide in rats. Methods Fifteen male SD rats were randomly divided into group A, B and C (5 rats in each group) according to random number table method. Rats in group A were administered 30 mg/kg Biapenem by tail vein, rats in group B were administered 30 mg/kg Biapenem and 3 mg/kg Methotrexate by tail vein, and rats in group C were administered 30 mg/kg Biapenem and 30 mg/kg Furosemide by tail vein. Plasma and urine were collected at different time points after administration. The concentrations of Biapenem in rat plasma and urine were determined by HPLC and the pharmacokinetic parameters were calculated. Results Compared with group A, area under the curve of Biapenem decreased, CL and CLr of Biapenem increased significantly (P ＜ 0.01) in group B; compared with group A, there was no significant difference in pharmacokinetic parameters of Biapenem in group C (P ＞ 0.05). Conclusion When Biapenem is administered combined with Methotrexate and Furosemide, Methotrexate can significantly reduce the exposure and increase the clearance of Biapenem, while Furosemide cannot significantly affect the pharmacokinetic of Biapenem in rats.
2019 Vol. 16 (31): 22-25 [Abstract] ( 270 ) HTML (1 KB)  PDF (666 KB)  ( 260 )
26 Study on the curative effect and the best concentration of Galium verum L. Solution on chronic skin ulcer in diabetic rats
YUAN Haigang1,2 ZHAO Gang2 JIA Zhen2 LYU Bochuan2 HUANG Yanhong2 ZHANG Bailiang2 JIANG Deyou2 WANG Xuejun1
Objective To explore the curative effect and the best therapeutic concentration of Galium verum L. Solution on chronic skin ulcer in diabetic rats. Methods Sixty healthy male Wistar rats were selected to establish diabetic model by intraperitoneal injection of streptozotocin. Random number table method was used to divided the Wistar rats into six groups: high concentration group (30 mg/mL), medium concentration group (3 mg/mL), low concentration group (0.3 mg/mL), negative control group, model control group and Befuji group, 10 per group. Treatment was taken on the second day after modeling. On the basis of routine dressing change on the wound, the treatment groups of the scutellaria chinensis were sprayed with the corresponding concentration of Galium verum L. Solution. The negative control group was sprayed with normal saline, while the Befuji group was sprayed with Befuji (concentration was 4200 U/mL). The transparent membrane was used to tracing film along the ulcer edge and the software was taken to calculate the remaining area on the third, seventh and tenth day after modeling. All the rats were sacrificed and local wound tissues were stained with HE to observe the pathological changes on the tenth day after modeling. Results The ulcer surface of each group decreased gradually with time. The wound areas of various concentrations of Galium verum L. groups and the Beifuji group were significantly smaller than those of the negative control group and the model control group at the same time point, and the differences were statistically significant (P ＜ 0.05). The wound area of various concentrations of Galium verum L. groups were significantly smaller than those of the Beifuji group at the same time point, while the wound area of medium concentration Galium verum L. group decreased most obviously, and the differences were statistically significant (P ＜ 0.05). There are interactions between different processing factors and time factors (P ＜ 0.05). The wound repair of beifuji group was better than that of negative control group and model control group, and each treatment group with different concentration of Galium verum L. Solution were superior to the Befuji group, while the wound healing and repair of the medium concentration group was the best, and the differences were statistically significant (P ＜ 0.05). Conclusion The Galium verum L. Solution is more effective than Beifuji in the treatment of skin ulcer in rats at the different points. And the therapeutic optimal concentration of Galium verum L. Solution in promoting wound healing in rats was 3 mg/mL.
2019 Vol. 16 (31): 26-29 [Abstract] ( 164 ) HTML (1 KB)  PDF (666 KB)  ( 67 )
30 Isolation and purification of verbascoside by macroporous resin
SU Ya1 KONG Zheng1 HUO Shixia2 JIANG Meng1 YAN Ming2
Objective To study the purification process of the glycoside in Cistanche tubulosa (Schenk) Wight by macroporous resin. Methods The content of glycosides in Cistanche tubulosa (Schenk) Wight was determined by high performance liquid chromatography (HPLC). Three kinds of kinetic equations were applied to fit the experimental data. The isotherm adsorption lines at different temperatures were fitted by Freundlich and Langmuir equations, and the thermodynamic parameters were calculated. Finally, the purification of glycosides in Cistanche tubulosa (Schenk) Wight by macroporous resin was investigated by single factor test. Results D101 macroporous resin was selected. The purification conditions were as followed: the concentration of sample solution was 1.5 mg/mL, the maximum sample loading rate was 6 BV, and the sample speed was 2 BV/h. It was eluted with 11 BV purified water and 30% ethanol of 10 BV. The content of glucoside in the flower was 25.1% after purification by D101 resin. Conclusion Under the conditions of this process, the purification of glycosides in Cistanche tubulosa (Schenk) Wight by D101 macroporous resin is simple and stable, and it can increase the content of glycosides in Cistanche tubulosa (Schenk) Wight.
2019 Vol. 16 (31): 30-33,41 [Abstract] ( 162 ) HTML (1 KB)  PDF (724 KB)  ( 47 )
34 Effect of VKORC1 and CYP2C9 gene polymorphism on the lasting dose and dose fluctuation at the initial anticoagulation phase
ZHANG Shuyu1,2 XU Zhe3 ZHOU Shouning2 ZHENG Zhuoling2 TANG Shifan2 ZHANG Xi3 HUANG Min2 LI Jiali2
Objective To investigate the effect of VKORC1 and CYP2C9 gene polymorphism on the lasting dose and dose fluctuation of warfarin at the initial anticoagulation phase (90 d after administration). Methods A total of 201 patients who underwent mechanical heart valve replacement in the First Affiliated Hospital, Sun Yat-sen University from June 2014 to December 2016 were recruited. The genotypes of VKORC1 rs7294 and CYP2C9*3 were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The effects of VKORC1 and CYP2C9 genotypes on daily warfarin lasting dose (LD) and daily warfarin dose difference (DD) were  analyzed. Results The mutation frequency of VKORC1 rs7294 C＞T and CYP2C9*3 was 12.5% and 2.5%, respectively, meeting the hardy weinberg equilibrium. There was no significant effect of CYP2C9*3 on LD and DD （P ＞ 0.05）. LD and DD of VKORC1 rs7294 T mutant carriers were significantly higher than that of CC wild homozygous carriers (P ＜ 0.001,P ＜ 0.01). Conclusion The mutation of VKORC1 rs7294 C＞T leads to the increase of warfarin anticoagulant lasting dose required by patients, which is more likely to cause dose fluctuation. Therefore, the monitoring of international standardized ratio should be strengthened.
2019 Vol. 16 (31): 34-36,45 [Abstract] ( 252 ) HTML (1 KB)  PDF (839 KB)  ( 157 )
37 Study on transdermal absorption and anti-inflammatory activity of Compound Sanguis Draxonis Microemulsion in vitro
DANG Xueliang1 JIA Lihua2 SONG Yanfeng1 ZHAO Jun1 WANG Qingwei1
Objective To investigate the transdermal absorption of main active components of Compound Sanguis Draxonis Microemulsion with different concentrations in vitro, and provide experimental basis for further study of the preparation. Methods According to the random number table, twenty-four SD rats were randomly divided into 3 groups, which were high-dose group (5.0%), medium-dose group (2.5%) and low-dose group (1.0%) of Compound Sanguis Draxonis Microemulsion, with 8 rats in each group. The isolated rat skin of the same part was taken as the permeation barrier, and the content of ligustilide, the main active ingredient of Compound Sanguis Draxonis Microemulsion, was determined by HPLC using modified Franz diffusion cell. The transdermal absorption of Compound Sanguis Draxonis Microemulsion was investigated by calculating the cumulative permeation amount of ligustilide in vitro. Forty SD rats were randomly divided into 5 groups according to their body weight, which were model group, Dexamethasone Acetate Cream group, high-dose group (5.0%), medium-dose group (2.5%) and low-dose group (1.0%) of Compound Sanguis Draxonis Microemulsion with 8 rats in each group. Experiment of paw swelling in rats induced by carrageenan was operated to observe inflammation inhibited by Compound Sanguis Draxonis Microemulsion, Dexamethasone Acetate Cream group as positive control group. Results The cumulative permeabilities of ligustilide, the main active ingredient of low-dose, medium-dose and high-dose groups of Compound Sanguis Draxonis Microemulsion, within 24 h were (153.000±6.586), (311.200±14.520) and (454.100±23.440) μg/cm2 respectively. The transdermal rate constants Jss were (6.8130±0.2196), (13.7100±0.5953), (20.1900±0.9491) μg/(cm2·h), respectively. Compared with the positive control group, the high-dose group of Compound Sanguis Draxonis Microemulsion significantly inhibited carrageenan-induced paw swelling in rats (P ＜ 0.01). Conclusion Compound Sanguis Draxonis Microemulsion has good transdermal performance and anti-inflammatory activity in vitro. The cumulative permeability per unit area within 24 h increased with the content of ligustilide, and the transdermal permeation behavior conformed to the zero-order kinetic equation.
2019 Vol. 16 (31): 37-41 [Abstract] ( 211 ) HTML (1 KB)  PDF (672 KB)  ( 67 )
42 Research progress on the relationship between bowel cleansing and intestinal microbiota
LI Qiang1 SUN Yi2 SHANG Yulong3
Adequate bowel cleansing is an important preoperative preparation for colonoscopy. With the deepening understanding of the association between intestinal microbiota and intestinal diseases. The influence of bowel cleansing on intestinal microbiota has also become an important factor for healthy people and patients with intestinal diseases to receive colonoscopy. Intestinal lavage fluids can directly wash intestinal microbiota, resulting a significant changes in intestinal microbiota structure in a short time. However, the long-term effects of different intestinal lavage fluids and different bowel cleansing schemes on intestinal microbiota remain unclear. In addition, the application of probiotics might play a potential role in the recovery of intestinal microbiota after bowel cleansing. Therefore, this paper focuses on the short-term and long-term effects of bowel cleansing on intestinal microbiota and discusses mechanism of microbiota recovery after receiving bowel cleansing. Demonstration of this issues and continuing in-depth research will help optimize cleansing solutions strategies.
2019 Vol. 16 (31): 42-45 [Abstract] ( 258 ) HTML (1 KB)  PDF (509 KB)  ( 71 )
46 Research progress on the correlation between high mobility group box chromosomal protein l and rheumatoid arthritis
LIU Wei1* HUANG Jian1* CHEN Caixia2 WANG Dan3
Rheumatoid arthritis is a chronic non-specific inflammatory disease of the joint synovium with joint lesions as the main manifestation, and the course of disease is progressive. High mobility group box chromosomal protein 1 is involved in the evolution and progression of various autoimmune diseases as a potential inflammation-inducing factor. And the abnormal expression of high mobility group box chromosomal protein 1 is closely related to the occurrence and development of rheumatoid arthritis. This article further elaborates and demonstrates that the expression of high mobility group box chromosomal protein 1 in serum of patients with rheumatoid arthritis promotes the up-regulation of inflammatory factor-related receptors, but its correlation with clinical indicators is still unclear. The expression of high mobility group box chromosomal protein 1 in synovial membrane in patients with rheumatoid arthritis promotes synovial inflammatory expression. High mobility group box chromosomal protein 1 promotes the synthesis and release of inflammatory cytokines, pathological proliferation, apoptosis, autophagy and irreversible progression of phenotypic transformation in synovial fibroblasts in rheumatoid arthritis. In theory, it provides a new target for the clinical diagnosis and disease intervention of rheumatoid arthritis.
2019 Vol. 16 (31): 46-51,55 [Abstract] ( 254 ) HTML (1 KB)  PDF (555 KB)  ( 80 )
52 Progress of diagnosis and treatment of transumbilical single site laparoscopy in gynecological operation
ZHANG Yayun LIANG Baoquan▲
Minimally invasive surgery has been a research hotspot in gynecological surgery in recent years. Transumbilical laparoscopic single site surgery has become one of the common research directions of many gynecologists because of its unique advantages, such as extreme minimally invasive and satisfactory surgical results. In recent years, with the constant struggle of many gynecologists in China and abroad to explore and improve, transumbilical laparoscopic single site surgery has gradually become mature. This article reviews the progress of transumbilical laparoscopic single site surgery in gynecology.
2019 Vol. 16 (31): 52-55 [Abstract] ( 217 ) HTML (1 KB)  PDF (505 KB)  ( 65 )
56 Research progress of therapies of Duchenne muscular dystrophy
YANG Yixian1 LI Changsheng1 WANG Beilei1 DANG Suying2
Duchenne muscular dystrophy (DMD) is a kind of X-linked recessive hereditary disease caused by mutations of the dystrophin gene. There is no effective therapy method at present. In recent years, with the increasing knowledge of the mechanisms and pathologic process of the disease, researchers have conducted studies of DMD therapy method and the effectiveness. These studies include developing pharmaceutical therapies for reducing inflammation, regulating calcium levels, promoting muscle growth and anti-fibrosis, developing auxiliary equipment to train or improve the life quality of patients, developing gene therapy strategies for DMD gene replacement, exon-skipping and gene editing, and investigating stem cell therapy. This paper summarizes the latest progress of each therapy for DMD.
2019 Vol. 16 (31): 56-59 [Abstract] ( 210 ) HTML (1 KB)  PDF (550 KB)  ( 84 )
60 Application of blending teaching based on rain classroom in the teaching of pathophysiology
LIU Huan WANG Shuang GUO Yufang HUO Jian GUO Chihua ZHAO Yan
The blending teaching based on rain classroom is a new teaching mode in the context of mobile internet and big data, which changes the traditional teaching patterns and methods. Based on the analysis of the function and characteristics of rain classroom, this paper introduces the construction of the blending teaching mode based on rain classroom in the teaching of pathophysiology, analyzes the teaching effect and discusses the advantages and disadvantages of this mode in teaching. Practice shows that the application of the blending teaching mode based on rain classroom in the teaching of pathophysiology truly realizes student-centered teaching, stimulates the enthusiasm and initiative of students, makes the communication between teachers and students more convenient and efficient, and improves the teaching quality.
2019 Vol. 16 (31): 60-63 [Abstract] ( 240 ) HTML (1 KB)  PDF (648 KB)  ( 73 )
64 Exploration and reflection on improving the comprehensive quality of medical students during clinical internship
LIU Yanquan1 SHEN Jianzhen1，2 FU Haiying1 CHEN Weihong3 ZHOU Huarong1，2
As a key period for the transition and transformation of medical students from the role of “student” to the role of “doctor”, the clinical internship period has an important impact on the career and life pattern of medical students. With the rapid development of social economy and medical science and technology, the medical education of China has also become modernized and internationalized. At the same time, the standards and requirements of the Chinese people in the medical industry are getting higher and higher, and the relationship between doctors and patients is becoming increasingly tense. As an intern, the internship is a young force in the medical fields. The improvement of its comprehensive quality is a hot spot of concern in today′s society, and it is also a problem worthy of exploration and consideration by various training units. This paper combines the current situation and existing problems of clinical practice teaching of undergraduates in medical colleges, through in-depth analysis of clinical, scientific research, humanities and other perspectives. Besides, it also discusses how to improve the comprehensive quality of medical students during clinical internships, and provides reference for the medical schools to improve the comprehensive quality of medical students during clinical practice.
2019 Vol. 16 (31): 64-68 [Abstract] ( 224 ) HTML (1 KB)  PDF (664 KB)  ( 43 )
69 Influence of Chinese folk martial arts inheritance teaching on Chinese medicine education
YE Xide1 LE Lixia1 HE Wei1 ZHOU Ming1 PENG Qiaozhen2 ZHANG Jiankang3
In order to discuss the influence of inheritance teaching of Chinese folk martial arts on the development of Chinese medicine education (CME). This paper discusses the influence of folk martial arts on the development of CME from its origin and relationship between folk martial arts and the development of CME. As for the aspects of teaching mode and effect, this paper analyzes the influence of Chinese folk martial arts on CME. Meanwhile, in view of the existing problems of this course in Chinese medicine colleges and universities, the author combines with his own teaching experience and proposes the development idea of integrating Chinese folk martial arts into CME, such as strengthening the construction of teaching materials, innovating teaching modes, strengthening the construction of teaching staff, and strengthening the course management and its capital investment. It is the consistency between the organic unity of mind and body movements emphasized by Chinese folk martial arts and the consistency of the overall concept emphasized by the basic theory of CME, which makes them correlation in personnel training, educational concepts and inheritance methods. It shows that the curriculum can promote the development of CME and folk martial arts, cultivate excellent talents in the field of CME for the society. While, it provides the possibility for the organic combination and in-depth development of the curriculum and traditional CME, and has a profound impact on the development of CME.
2019 Vol. 16 (31): 69-72 [Abstract] ( 204 ) HTML (1 KB)  PDF (652 KB)  ( 63 )
73 Practice and exploration of the trinity teaching model reform of parasitology inspection under the background of five-year to four-year reform
ZHAO Liping1 WU Qian1 WANG Li2 MA Tianyi1 LI Yankui1 QUAN Zhibo1
Within the context of the policy of adjusting the five-year to four-year reform for medical laboratory science, and in respect of a variate of problems that exist in the teaching of parasitological laboratory medicine in Shaanxi University of Chinese Medicine, such as the out of date teaching content, the dull teaching form, the defective evaluation mechanism, this article proposes the reform of the teaching mode from three dimensions, namely the teaching content and teaching method, the practice teaching and the evaluation method. Based on the characteristics of medical laboratory technology, the article introduces a lot of specific measures to carry out the reform, including adjusting the teaching content, focusing on the key subjects, improving the teaching form, enriching the teaching method (this involves the flexible use of teaching methods, conducting the seminar, building the interactive bridge, and adding the ideological and political education), strengthening the teaching practice, cultivating students′ comprehensive ability and innovation consciousness (by building a digital specimen resource pool, adding more comprehensive and design experiments, conducting social practice teaching, encouraging more involvement in scientific research and training), reforming the evaluation method, and diversifying the assessment indicators. The objectives of this article are to mobilize students and enhance their enthusiasm in learning, to help them master the basic theoretical knowledge of parasitological laboratory medicine in a limited period, to improve their operational skills, to give full play to their innovation, to cultivate their practical and problem-solving ability, and to provide reference on reforming the teaching of parasitological laboratory medicine.
2019 Vol. 16 (31): 73-76,87 [Abstract] ( 194 ) HTML (1 KB)  PDF (712 KB)  ( 60 )
77 The psychological health education for college students under new media environment
CHEN Yiting1 ZHANG Hui2
As the result of the rapid development of science, new media frequently appears in people′s daily life. College students are the main force in using new media who′s lives have a close contact with it. Meanwhile, in recent years, mental illness has an increasing impact on people′s physical and mental health. How to alleviate and release psychological pressure has become a priority. New media, as a new scientific means, has an extraordinary impact on the mental health of college students, with both advantages and disadvantages. On the one hand, the new media can promote the development and popularization of mental health education, help college students solve psychological illness or alleviate negative emotions caused by study and life. On the other hand, improper use of new media can easily make college students indulge in virtual environment and produce new or aggravate the original mental illness. By analyzing the double effects of new media on college students′ mental health, we can prescribe remedies for the illness and find ways to optimize psychological education by using new media, such as establishing a psychological education network platform, developing psychological education from online to offline, so as to help students develop physically and mentally well. This article discusses the impacts for college students′ psychological health, as well as proposes some suggestions on mental health education under new media environment.
2019 Vol. 16 (31): 77-80 [Abstract] ( 177 ) HTML (1 KB)  PDF (760 KB)  ( 92 )
81 Study on the influencing factors of satisfaction degree of traditional Chinese medicine culture integrating into humanities and social sciences education
LI Ang LI Baoqin
Objective To understand the students′ satisfaction with the integration of traditional Chinese medicine culture into humanities and social sciences education, analyze the influencing factors, and provide suggestions for improving teaching quality and strengthening management. Methods Undergraduate students from Heilongjiang University of Chinese Medicine, Guangzhou University of Chinese Medicine, Zhejiang Chinese Medical University and Tianjin University of Traditional Chinese Medicine were selected by convenience sampling to conduct self-designed questionnaire survey in January and February 2018. Multiple linear regression was used to analyze the influencing factors of students′ satisfaction with the integration of traditional Chinese medicine culture into humanities and social sciences education. Results Whether the teachers of humanities and social sciences courses have the corresponding knowledge of traditional Chinese medicine culture, whether the content of traditional Chinese medicine culture in the courses they have learned is sufficient, and whether the school attaches importance to the classroom education of traditional Chinese medicine culture had impacts on the students′ satisfaction, and the differences were highly statistically significant (all P ＜ 0.001). Conclusion In order to improve the students′ satisfaction, the school should pay more attention to improving the traditional Chinese medicine cultural knowledge level of the teachers who teach humanities and social sciences courses, and enhance the integration of traditional Chinese medicine cultural teaching content in humanities and social science education, so as to improve the humanistic quality of medical students.
2019 Vol. 16 (31): 81-83,102 [Abstract] ( 190 ) HTML (1 KB)  PDF (971 KB)  ( 66 )
84 Exploration on the cultivating of clinical thinking ability of medical students by Zhiqu App
HUANG Guotao HONG Li HU Ming ZHOU Min LU Danhua
Our country aims at cultivating clinical application-oriented medical talents with good clinical thinking and meeting social needs. Early cultivating of scientific clinical thinking mode is the primary goal of every medical student and clinical worker. This paper analyzes the shortcomings of the current teaching schemes, discusses the application of Zhiqu APP to teaching, integrates artificial intelligence technology with the teaching system, maximizes the use of massive data resources in the medical field, and completely restores clinical diagnosis and treatment, including consultation, physical examination, auxiliary examination, diagnosis and treatment, aiming to cultivate scientific and healthy clinical thinking in the early stage and provide theoretical basis for the reform of teaching system.
2019 Vol. 16 (31): 84-87 [Abstract] ( 173 ) HTML (1 KB)  PDF (654 KB)  ( 92 )
88 Meta-analysis of the efficacy of glucocorticoid in the treatment of autoimmune thyroiditis
YIN Xietian1,2,3 ZHAO Shichao4 XIANG Nan1,3 ZHOU Guangwen3,5 ZENG Mingxing3,6
Objective To systematic review the efficacy and safety of glucocorticoid in the treatment of autoimmune thyroiditis (AITD) through Meta-analysis. Methods Through the Cochrane Collaborative Network Manual, the retrieval strategy is formulated and the literature quality is evaluated. By searching the databases of CNKI, Wanfang, VIP, CBM, PubMed, Embase, Cochrane Library, SCI and Clinical trial were searched for randomized controlled trials of glucocorticoid for autoimmune thyroiditis published before March 20, 2019. Revman 5.3 was used for Meta-analysis. Results A total of 15 studies were included. The Meta-analysis showed that: the thyroid peroxidase antibody (TPOAb) in the treatment group was significantly lower than that in the control group, and the difference was statistically significant (SMD=-1.41, 95%CI：-1.97, -0.85, P ＜ 0.05). The thyroglobulin antibody (TGAb) in the treatment group was significantly lower than that in the control group, and the difference was statistically significant (SMD=-1.51, 95%CI：-2.11, -0.91, P ＜ 0.05). The thyroid volume in the treatment group was significantly smaller than that in the control group, and the difference was statistically significant (SMD=-1.66, 95%CI：-1.94, -1.37, P ＜ 0.05). There was no significant difference in the incidence of adverse reactions between two groups (OR=0.38, 95%CI：0.06, 2.51, P ＞ 0.05). Conclusion Glucocorticoid treatment of AITD has a significant effect. It can reduce the autoantibody level of AITD and the thyroid volume. Moreover, it has short-term or local application, small adverse reactions, and it can be used clinically.
2019 Vol. 16 (31): 88-93 [Abstract] ( 223 ) HTML (1 KB)  PDF (1546 KB)  ( 139 )
94 Relationship between antibiotic susceptibility and genotype of Mycobacterium abscessus clinical isolates
MAO Yanhua CHU Haiqing
Objective To observe the relationship between antibiotic susceptibility and genotype of clinical isolates of Mycobacterium abscessus. Methods From January 2016 to September 2018, 87 strains of Mycobacterium abscess isolated from Shanghai Pulmonary Hospital were selected for the study. Among them, 72 strains were sputum and 15 strains were bronchoalveolar lavage fluid. All strains were identified as Nontuberculous mycobacteria by MGIT960 medium growth and p-nitrobenzoic acid test, while further identified as Mycobacterium abscessus by sequencing rPOB gene. All strains were subjected to quantitative polymerase chain reaction amplification, while single nucleotide polymorphism analysis was performed using NCBI Nucleotide blast program. Antibiotic sensitivity was determined by microdilution method, while the relationship between antibiotic sensitivity and genotype was analyzed. Results Eighty-seven isolates included 81 subsp.abscessus, 6 subsp.massiliense. A total of 72 ERM (41) T28 sequences and 9 ERM (41) C28 sequences in 81 subsp.abscessus, and ERM (41) sequences to 10 species were sensitivity to antibiotics. 81 subsp.abscessu subtypes included 41 Claroycin (CLA) -sensitive strains and 10 CLA-mediated strains, 8 ERM (41) C28 and 43 ERM (41) T28 isolates without rrl 2058/2059 mutation. 30 resistant isolates were consisted of 29 ERM (41) T28 isolates (5 of them had rrl 2058/2059 mutations) and 1 ERM (41) C28 isolate (1 occurred the rrl 2058/2059 mutation). There were 2 subsp.massiliense resistant isolates, and 1 had a rrl 2058/2059 mutation. Conclusion The rrl and ERM (41) genotypes have a certain predictive effect on CLA resistance of clinical isolates of abscess abacillus. The treatment of Mycobacterium abscessus with Amikacin may have better effect and it is worthy of further study.
2019 Vol. 16 (31): 94-97 [Abstract] ( 256 ) HTML (1 KB)  PDF (489 KB)  ( 48 )
98 Effect of Etomidate continuous infusion on perioperative hemodynamics and adrenocortical function in elderly patients undergoing total hip arthroplasty combined with lumbosacral plexus block
GONG Wenyi1 XU Bing1 ZOU Feng2 YUE Xiaofang2 FAN Kun2
To investigate the safety and feasibility of Etomidate administered by continuous infusion combined with lumbosacral plexus block in elderly patients undergoing total hip arthroplasty. Methods From January to December 2018, 102 elderly patients undergoing total hip arthroplasty in Baoshan Branch of Shanghai First People′s Hospital were randomly divided into the following groups: Propofol induction + Sevoflurane maintenance inhalation group (group A), Etomidate induction + maintenance infusion group (group B). Lumbosacral plexus block was performed in both groups before induction of general anesthesia. The recovery time, the use of deoxyepinephrine, etc. were recorded. Heart rate (HR), mean arterial pressure (MAP), stroke volume (SV), and systemic vascular resistance (SVR) were measured and recorded before anesthesia induction (T1), after anesthesia induction (T2), at the beginning of operation (T3), before reaming cavity (T4), after femoral head prosthesis placement (T5), and at the end of operation (T6). The concentration of cortisol (COR) and adrenocorticotropic hormone (ACTH) were measured before anesthesia (t0), at the end of operation (t1), 24 h after operation (t2), 48 h after operation (t3). The occurrence of adverse reactions was recorded. Results There were 92 patients were enrolled in the study: 45 patients in group A and 47 patients in group B. There was no significant difference between the two groups in operative time, removal time of laryngeal mask after operation and bleeding volume (P > 0.05). The recovery time of group B was significantly longer than that of group A (P < 0.01). The number and total amount of deoxyepinephrine used in group A were significantly higher than those in group B (P < 0.01). There was no significant difference in HR and SV between the two groups at different time points during operation (P > 0.05). MAP and SVR were significantly higher in group B than in group A at T2-T5 (P < 0.01). MAP, SV and SVR in group A decreased at T2-T5 compared with T1 (P < 0.05), while MAP in group B decreased at T5 (P < 0.01). At t1, COR in group B was lower than that in group A (P < 0.01), and ACTH was higher than that in group A (P < 0.01). In group B, COR decreased at t1 compared with t0 (P < 0.01) and ACTH increased at t1 compared with t0 (P < 0.01). There was no significant difference in COR and ACTH in group A at t1-t3 (P > 0.05). The incidence of injection pain, post-operative restlessness and nausea and vomiting in group B were lower than that in group A (P < 0.05), but the incidence of myoclonus was higher than that in group A (P < 0.05). Conclusion Etomidate induction and maintenance has little effect on hemodynamics in elderly patients undergoing total hip arthroplasty on the basis of lumbosacral plexus block, the inhibition of Etomidate on adrenocortical function is transient. It is advantageous to anesthesia safety.
2019 Vol. 16 (31): 98-102 [Abstract] ( 170 ) HTML (1 KB)  PDF (451 KB)  ( 36 )
103 Effect of Sevoflurane combined with Remifentanil anesthesia on stress response and hemodynamics in patients undergoing laparoscopic hysteromyomectomy
AI Jinlian XIAO Jinhui LIU Rong WANG Minhua GUI Xinxing
Objective To study the effects of Sevoflurane combined with Remifentanil anesthesia on stress response and hemodynamics in patients undergoing laparoscopic hysteromyomectomy. Methods Eighty-six female patients who were scheduled to undergo laparoscopic hysteromyomectomy in 908th Hospital of the Joint Logistics Support Force of the Chinese People′s Liberation Army from October 2016 to October 2018 were selected as the study subjects. They were divided into the study group and the control group according to the random number table method, 43 cases in each group. The control group was anesthetized by Remifentanil combined with Propofol, while the study group was anesthetized with Remifentanil combined with Sevoflurane. The levels of cortisol (COR), adrenaline (A), norepinephrine (NA), C-reactive protein (CRP), and blood glucose (GLU) at 5 min before anesthesia (T0), 30 min after pneumoperitoneum (T1), the end of operation (T2), postoperative 2 h (T3), and hemodynamic parameters such as heart rate (HR), mean arterial pressure (MAP), systolic blood pressure (SBP) and oxygen saturation (SpO2) at T0, T1 and T2, and the incidence of postoperative adverse reaction was compared between the two groups. Results The levels of COR, A, NA and GLU at T0-T3 in the two groups were increased at first and then decreased, while those at T1 and T2 of the study group were lower than those of the control group (P < 0.05); the CRP level of the two groups showed an increasing trend at each time period, and the study group was lower than that of the control group in the same period (P < 0.05). HR, MAP and SBP at T0 - T2 was increased at first and then decreased, while those at T1 and T2 of the study group were lower than those of the control group (P < 0.05). There was no significant difference in SpO2 level between the two groups at all stages (P > 0.05). The incidence of postoperative adverse reactions in the study group (13.95%) was lower than that in the control group (32.56%) (P < 0.05). Conclusion Sevoflurane combined with Remifentanil anesthesia in laparoscopic hysteromyomectomy can effectively reduce the stress reaction and hemodynamic fluctuation of patients during operation, and reduce the occurrence of adverse reactions after operation.
2019 Vol. 16 (31): 103-106 [Abstract] ( 202 ) HTML (1 KB)  PDF (439 KB)  ( 91 )
107 Discussion on the teaching experience of “analogical selection of Chinese and foreign drugs” in dermatology teaching of traditional Chinese medicine based on image pharmacology
XU Jingna1 DENG Bingxu1 XU Xuying2 SUN Liyun1
The teaching group of Beijing Chinese Medicine Hospital, Capital Medical University, in the teaching of traditional Chinese medicine dermatology, grasps the characteristics of traditional Chinese medicine dermatology, based on the theory of “faxiang pharmacology”, and the “analogical selection of Chinese and foreign drugs” Chinese medicine external treatment. The method is an innovative teaching method, and at the same time, the teaching mode is rich in fun and stereoscopic image. The research content includes the understanding of various characteristics of the corresponding surgical external treatment applied to the treatment of the disease, the application steps of the specific therapy, the familiarity of the features and the corresponding. In order to adapt to the taboos, the above research results are summarized and analyzed into classroom and practice teaching, and achieve good teaching results.
2019 Vol. 16 (31): 107-110 [Abstract] ( 166 ) HTML (1 KB)  PDF (706 KB)  ( 101 )
111 Professor Cui Shusheng′s experience summary in treating neurological insomnia with angle medicine theory
LIU Dianlong1 DING Honglei2 CUI Shusheng2
Professor Cui Shusheng believes that neurological insomnia is a common and frequently-occurring disease, and belongs to the category of internal medicine. The clinical application of Chaihu series prescription, Shenqi Pill series prescription and Suanzaoren Decoction as the basis of the prescription, and multiple angle drug groups for symptomatic addition and subtraction. Professor Cui believes that there are four main types of neurological insomnia. The disease is located in the heart and brain, and is closely related to the liver, spleen and kidney. The overall syndrome is not separated from the yin and yang camp, the treatment starts from the whole, the heart and kidney are treated together, the qi and blood are adjusted, and the care is combined. Based on professor Cui′s understanding of the etiology and pathogenesis of neurological insomnia, the authors summarize the experience of professor Cui Shusheng′s use of horn medicine theory in the treatment of neurological insomnia in terms of clinically used main parties, addition and subtraction, and clinical typical cases.
2019 Vol. 16 (31): 111-114 [Abstract] ( 193 ) HTML (1 KB)  PDF (756 KB)  ( 50 )
115 Clinical efficacy of modified Chinese medicine iontophoresis in the treatment of nonalcoholic fatty liver disease
YIN Xiaofei SUN Min LI Huiling LU Gendi
Objective To investigate the effect of modified Chinese medicine iontophoresis on patients with nonalcoholic fatty liver disease. Methods Seventy-two patients with nonalcoholic fatty liver disease were selected from July 2017 to May 2018 in Department of Liver Disease, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine. According to the random number table method, they were randomly divided into control group and observation group, with 36 cases in each group. Control group was treated with conventional Chinese medicine iontophoresis on the basis of conventional treatment, while observation group was treated with improved Chinese medicine iontophoresis. Body mass index (BMI), traditional Chinese medicine syndrome integral, plasma total cholesterol, high-density lipoprotein, low-density lipoprotein, triglyceride levels and CAP value were compared between two groups. Results BMI and traditional Chinese medicine syndrome scores of two groups after treatment were significantly lower than before treatment (P < 0.05), but there was no significant difference between two groups after treatment (P > 0.05). The levels of total cholesterol, low-density lipoprotein and triglyceride in two groups were significantly lower than those before treatment (P < 0.05), and high-density lipoprotein was significantly higher than before treatment (P < 0.05). Triglyceride in observation group was lower than that in control group after treatment, the difference was statistically significant (P < 0.05). There was no significant difference in the levels of total cholesterol, high-density lipoprotein and low-density lipoprotein between two groups after treatment (P > 0.05). The CAP value of liver in two groups after treatment was significantly lower than that before treatment, observation group was significantly lower than control group, and the differences were statistically significant (P < 0.05). Conclusion Modified Chinese medicine iontophoresis can improve the curative effect of nonalcoholic fatty liver disease, and has the value of promotion.
2019 Vol. 16 (31): 115-118 [Abstract] ( 177 ) HTML (1 KB)  PDF (755 KB)  ( 93 )
119 The example of Professor Zhao Shuhua′s experience in treating gastricepigastralgia
LIU Minghui ZHAO Shuhua XUE Junlai
Professor Zhao Shuhua, a famous doctor of Chinese medicine in Jilin Province, is a tutor who inherits the academic experience of the sixth batch of national experts of Chinese medicine. He has been a doctor for more than 40 years, and has plenty of experience in the treatment of digestive system diseases. The entry points of this article are five common pathogenesis, including liver-qi invading stomach, heat stagnation in liver and stomach, deficient cold of spleen and stomach, obstruction of middle jiao by damp-heat and deficiency of stomach-yin. Furthermore, he clarify the cognition and the diagnosis of epigastralgia by means of the combination of the pathogenesis and the clinical symptoms.
2019 Vol. 16 (31): 119-122 [Abstract] ( 210 ) HTML (1 KB)  PDF (707 KB)  ( 202 )
123 Effect of Niuhuang Ninggong Tablets combined with Aripiprazole on schizophrenia
LEI Xiuping1 GUO Yan1 ZHAO Lingdan2 XU Xiaoming3
Objective To investigate the clinical efficacy of Niuhuang Ninggong Tablets combined with Aripiprazole in the treatment of schizophrenia. Methods One hundred schizophrenic patients admitted to Sichuan Province Zigong Mental Health Center from February 2017 to October 2018 were selected. They were divided into control group and study group with 50 cases in each group by random number table method. The control group was treated with Aripiprazole, while the study group was added Niuhuang Ninggong Tablets on the basis of the control group. The clinical efficacy, psychiatric symptoms, depression, quality of life, related serological indicator and adverse reaction were compared between the two groups. Results The total effective rate of the study group was significantly higher than that of the control group, and the difference was statistically significant (P ＜ 0.05). After 6 weeks of treatment, the score of positive and negative symptoms scale (PANSS) and Hamilton depression scale (HAMD) in the two groups were decreased, while those in the study group were lower than the control group, and the differences were statistically significant (P ＜ 0.05); the score of general quality of life inventory-17 (GQOLI-17) was increased, while the study group was higher than that of the control group, and the differences were statistically significant (P ＜ 0.05). After 6 weeks of treatment, the Neuron-specific enolase (NSE) and S100β protein in the two groups were decreased, while the study group were lower than those in the control group, and the differences were statistically significant (P ＜ 0.05); brain-derived neurotrophic factor (BDNF) was increased, while the study group was higher than that in the control group, and the differences were statistically significant (P ＜ 0.05). There was no significant difference in the total incidence of adverse reactions between the two groups (P ＞ 0.05). Conclusion Niuhuang Ninggong Tablets combined with Aripiprazole in the treatment of schizophrenia has definite curative effect. It can effectively improve the serum NSE, S100β and BDNF levels of patients, and does not increase the incidence of adverse reactions. It has a certain clinical application value.
2019 Vol. 16 (31): 123-126 [Abstract] ( 221 ) HTML (1 KB)  PDF (709 KB)  ( 68 )
127 Relation between traditional Chinese medicine constitution and prevention and treatment of malignant tumor
ZHAO Hongbo GONG Weijun
Malignant tumor has now become the main disease that threatens human health and causes death. How to prevent and treat cancer disease is a common problem faced by human beings. The constitution is closely related to the occurrence, development, and prognosis of malignant tumor. Therefore, the prevention and treatment of tumor disease also needs the participation of traditional Chinese medicine (TCM) constitutional theory. The identification of TCM constitution has great significance for the prevention, treatment and rehabilitation of malignant tumor. This article focuses on the relationship between tumor disease and lopsided-constitution, and how to apply TCM to regulate and improve TCM constitution, so that it tends to be normal-constitution. Normal-constitution is not only beneficial to the prevention of tumor diseases, but also has a certain effect on the rehabilitation of tumor diseases as well as reduce the side effects of chemotherapy. It is worthy of further clinical research.
2019 Vol. 16 (31): 127-130 [Abstract] ( 199 ) HTML (1 KB)  PDF (710 KB)  ( 90 )
131 Clinical observation on treatment of rheumatoid arthritis with Wujin Qushi Decoction
LI Juanhong1 LIU Hongfu1 LI Guiying2
Objective To observe the clinical effect of Wujin Qushi Decoction on patients with rheumatoid arthritis (RA). Methods A total of 87 patients with RA admitted to Beijing Geriatric Hospital from March 2014 to March 2015 met the inclusion criteria were selected. They were divided into the observation group (44 cases) and the control group (43 cases) randomly according to the random number table method. The observation group was given Wujin Qushi Decoction once a day in the morning and once in the evening, each time taking 10 mL orally. And the control group was given Methotrexate 10 mg once a week. Both groups had treatment for 8 weeks. During the treatment, VAS score, number of joint pain, swollen joint count, morning stiffness duration, adverse reactions, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), rheumatoid factor (RF) and the use of non-steroidal anti-inflammatory drug (NSAIDs) were measured. Results After the treatment, the control rate of the observation group was higher than that of the control group, the difference was statistically significant (all P ＜ 0.05). Compared with before treatment, VAS score, number of joint pain, swollen joint count, morning stiffness duration, ESR, CRP, RF and other laboratory indicators in the two groups were all reduced after treatment (P ＜ 0.05), and the observation group was lower than control group (P ＜ 0.05). The use rate of non-steroidal anti-inflammatory drugs and incidence of adverse reactions in the observation group were lower than those in the control group, and the differences were statistically significant (P ＜ 0.05). Conclusion Wujin Qushi Decoction is effective in the treatment of rheumatoid arthritis, and has more advantages than Methotrexate in relieving clinical symptoms and signs, and the adverse reactions are less than Methotrexate.
2019 Vol. 16 (31): 131-134 [Abstract] ( 156 ) HTML (1 KB)  PDF (711 KB)  ( 78 )
135 MRI-based three-dimensional reconstruction of pelvic levator ani muscle and bony pelvis on male patients with rectal prolapse
CUI Guoce1 LI Yufei2 LI Huashan2
Objective To study the three-dimensional reconstruction of pelvic levator supporting tissue (levator ani muscle and bony pelvis) based on magnetic resonance imaging (MRI), and to observe and measure the parameters of two-dimensional MRI images and three-dimensional models of levator ani muscle and pelvic reconstruction. Methods From October 2016 to December 2018, 11 male patients admitted to Guang′anmen Hospital and South District, Guang′anmen Hospital, China Academy of Chinese Medical Sciences with rectal prolapse as the main diagnosis were selected as the observation group, and 13 healthy male volunteers were recruited as the control group at the same time. MRI scan was performed in both groups to observe original data on levator ani muscle hiatus shape, iliococcygeus form and break of levator ani muscle. Mimics 19.0 three-dimensional reconstruction software was used for three-dimensional reconstruction, and morphological characteristics of three-dimensional models of levator ani muscle and bony pelvis were observed. The levator hiatus width (LHW), levator hiatus length (LHL), levator symphysis gap of left and right side (LSG-L, LSG-R), levator plate angle (LPA), levator ani muscle volume (LVOL), iliococcygeus width of left and right side (ICW-L, ICW-R), iliococcygeal angle of left and right side (ICA-L, ICA-R), pubococcygeal line (PCL), pubic arch angle (PAA), interspinous diameter (IS), intertuberous diameter (IT) were compared and measured. Results Compared with the control group, the levator ani muscle hiatus shape, iliococcygeus form in the observation group were changed to some extent, and the levator ani muscle had a large degree of rupture defect. Compared with the control group, LHW, LHL, LSG-L, LSG-R, LPA were increased, LVOL was decreased in the observation group, the differences were statistically significant (P < 0.05). The differences of ICW-L, ICW-R, ICA-L, ICA-R between the two groups were not statistically significant (P > 0.05). PCL and IT in the observation group were higher than those of the control group, the differences were statistically significant (P < 0.05). The differences of IS and PAA between the two groups were not statistically significant (P > 0.05). Conclusion MRI two-dimensional and three-dimensional data sets reflect the spatial structure and adjacent relationship of pelvic levator ani muscle between healthy men and male patients with rectal prolapse, and evaluate the functional status of pelvic levator supporting structure, which provides an important reference for clinical classification diagnosis, surgical method selection and postoperative efficacy evaluation.
2019 Vol. 16 (31): 135-140 [Abstract] ( 187 ) HTML (1 KB)  PDF (870 KB)  ( 58 )
141 Quantitative analysis of benign and malignant thyroid nodules under contrast-enhanced ultrasound
CHEN Jie1 ZHENG Li1 WANG Meiyu1 ZHOU Dawei1 DU Jing2 JIANG Xinhui1 XIN Jun1
Objective To explore the quantitative analysis of benign and malignant thyroid nodules under contrast-enhanced ultrasound. Methods From October 2016 to December 2018, 60 patients with a total of 62 thyroid nodules who proposed to be operated on Shanghai Baoshan District Hospital of Traditional Chinese and Western Medicine were selected, and all patients obtained definite pathological results. All patients were treated with contrast-enhanced ultrasound examination and time-intensity curve (TIC) parameters in thyroid nodules and surrounding normal tissues were quantitatively analysed. The difference of peak intensity (ΔPI), the difference of Slope (ΔSlope) and the difference of area under the curve (ΔAUC) were recorded. The ROC curve were drew to evaluate the value of differential diagnosis of benign and malignant thyroid nodules. Results When the cut-off values of ΔPI, ΔSlope and ΔAUC in differential diagnosis of benign and malignant thyroid nodules were -3.25, -1.27, -89.65, the sensitivity were 83%, 75%, 75% and the specificity were 72%, 57%, 77%, respectively. Conclusion Quantitative analysis of TIC curve parameters in contrast-enhanced ultrasound may be helpful in differential diagnosis of benign and malignant thyroid nodules.
2019 Vol. 16 (31): 141-143,147 [Abstract] ( 196 ) HTML (1 KB)  PDF (579 KB)  ( 113 )
144 Clinical application value of vaginal color Doppler ultrasound combined with real-time three-dimensional hysterosalpingography in the diagnosis of hydrosalpinx
CHEN Cai1 ZHANG Qin1 SUN Yuxi1 WANG Wenjuan1 LU Yixun1 WANG Xingmin2▲
 Objective To explore the clinical application value of svaginal color Doppler ultrasound combined with real-time three-dimensional hysterosalpingography (RT-3D-HyCoSy) in the diagnosis of hydrosalpinx. Methods From November 2015 to October 2018, 60 cases of infertile women with hydrosalpinx diagnosed by vaginal color Doppler ultrasound in Guangxi Zhuang Autonomous Region Liuzhou Maternal and Child Health Care Hospital were selected. Vaginal color Doppler ultrasound, RT-3D-HyCoSy, vaginal color Doppler ultrasound combined with RT-3D-HyCoSy methods were performed, and the results of the three methods were compared and analyzed. Laparoscopic surgery was served as golden standard. The sensitivity, specificity, positive predictive value and negative predictive value of the three methods were compared. Results The sensitivity, specificity, positive predictive value and negative predictive value of vaginal color Doppler ultrasound combined with RT-3D-HyCoSy were higher than those of vaginal color Doppler ultrasound, and sensitivity and negative predictive value were higher than those of RT-3D-HyCoSy. The area under the ROC curve was 0.995. Kappa test results showed that the consistency and coincidence rate of vaginal color Doppler ultrasound combined with RT-3D-HyCoSy was the highest. Conclusion RT-3D-HyCoSy can dynamically observe the shape of fallopian tube, hydrosalpinx and its relationship with surrounding tissues. The sensitivity and accuracy of the diagnosis of hydrosalpinx can be improved when vaginal color Doppler ultrasound combined with RT-3D-HyCoSy. It can provide valuable basis for clinical selection and formulation of reasonable treatment program.
2019 Vol. 16 (31): 144-147 [Abstract] ( 208 ) HTML (1 KB)  PDF (552 KB)  ( 104 )
148 Effect of Jinbei Tankeqing Particles combined with Diprophylline in the treatment of elderly patients with acute exacerbation of chronic bronchitis
HUANG Ying1 LIU Qiongjiao1 GONG Zonglian2
Objective To explore clinical efficacy and safety of Jinbei Tankeqing Particles combined with Diprophylline in the treatment of elderly patients with acute exacerbation of chronic bronchitis. Methods From January 2017 to January 2019， 109 cases of elderly patients with acute exacerbation of chronic bronchitis in Nanchong Mental Health Center (the Second People′s Hospital of Nanchong City) were selected. According to the random number table method, they were divided into control group (n = 54) and observation group (n = 55). Patients of control group were treated with Diprophylline, while observation group were treated with Jinbei Tankeqing Particles combined with Diprophylline, both groups were treated for 2 weeks. Clinical curative effect was evaluated after treatment. The cough up phlegm, cough disappeared, then sound and wheezing disappeared times of the two groups were compared. The arterial partial pressure of oxygen (PaO2), oxyhemoglobin saturation (SpO2), partial pressure of carbon dioxide in artery (PaCO2) and serum levels of interleukin (IL)-4, IL-6, tumor necrosis factor-α (TNF-α) of the two groups before and after treatment were compared. The adverse reactions of the two groups were recorded. Results The total clinical effective rate of observation group was higher than that of control group, the difference was statistically significant (P < 0.05). The cough up phlegm, cough, then sound and wheezing disappeared times of observation group were significantly less than those of control group, the differences were statistically significant (P < 0.05). After treatment, the PaCO2 of two groups were significantly lower than those before treatment, the PaO2, SaO2 of the two groups were significantly higher than those before treatment, the differences were statistically significant (P < 0.05). And the PaO2, SaO2, PaCO2 of observation group were significantly greater than those of control group, the differences were statistically significant (P < 0.05). After treatment, the serum levels of IL-4, IL-6, TNF-α of the two groups were significantly lower than before treatment, and those of observation group were lower than control group, the differences were statistically significant (P < 0.05). Conclusion Jinbei Tankeqing Particles combined with Diprophylline in the treatment of elderly patients with acute exacerbation of chronic bronchitis has a good efficacy and low adverse reactions. It can improve the clinical symptoms and blood gas analysis indexes, reduce the inflammatory factor levels.
2019 Vol. 16 (31): 148-151 [Abstract] ( 176 ) HTML (1 KB)  PDF (370 KB)  ( 76 )
152 Performance evaluation of two modules of XN9000 hematology analyzer
ZHAO Yan YU Chao NI Wei
Objective To verify and evaluate the performance of SysmexXN9000 automatic hematology analyzer with two analysis modules. Methods According to “Health Standard of the People′s Republic of China WS/T406-2012”, performance of SysmexXN9000 (A1+B4) automatic blood analyzer for complete blood count, leukocyte classification, reticulocyte count, etc was verification and evaluated. Results Blank counting, carrying contamination rate, precision (within and between batches), accuracy and linearity all meet the standard requirements. Leukocyte classification and reticulocyte were correlated with manual count (P ＜ 0.01), and the correlation coefficients of A1 and B4 modules were 0.675 and 0.678, respectively, which were smaller than other parameters. Conclusion The SysmexXN9000 (A1+B4) automatic blood analyzer has good performance and high clinical value.
2019 Vol. 16 (31): 152-155,168 [Abstract] ( 173 ) HTML (1 KB)  PDF (760 KB)  ( 36 )
156 Changes of serum 25(OH)D3 and IgE level in children with respiratory syncytial virus bronchiolitis and their clinical significance
LIANG Yujuan LUO Xuebin LIU Limin ZOU Yan
Objective To study the changes of serum 25(OH) D3 and immunoglobulin E (IgE) level in children with respiratory syncytial virus (RSV) bronchiolitis and their clinical significance. Methods From January 2016 to January 2019, 100 children with RSV bronchitis admitted to Liuzhou Workers′ Hospital of Guangxi Zhuang Autonomous Region (hereinafter referred to as "our hospital") were selected as the lesion group. In addition, 100 healthy children who underwent physical examination in our hospital at the same time were selected as the control group. Serum 25(OH) D3 and IgE levels in the two groups were detected by Enzyme-linked Immuno Sorbent Assay. In addition, according to the grading scheme, the children in the lesion group were divided into 25 cases in the mild lesion group, 48 cases in the moderate lesion group and 27 cases in the severe lesion group. The serum 25(OH)D3 and IgE levels of the three groups were compared and the correlation was analyzed. Results Serum 25(OH)D3 level in the lesion group was lower than that in the control group, while serum IgE level was higher than that in the control group (all P ＜ 0.05). Serum 25(OH)D3 level of RSV bronchitis children in mild, moderate and severe lesion groups decreased gradually, while serum IgE level increased gradually. The statistically significant differences among the groups (all P ＜ 0.05). The severity of RSV bronchitis in children was negatively correlated with serum 25(OH)D3 level (r = -0.629, P ＜ 0.05). It was positively correlated with serum IgE level (r = 0.604, P ＜ 0.05). Conclusion The serum level of 25(OH)D3 in children with RSV bronchiolitis is significantly low expression, while the serum level of IgE is significantly high expression. The above two indicators may be jointly detected in clinical work to help determine the severity of the disease in children with RSV bronchiolitis.
2019 Vol. 16 (31): 156-159 [Abstract] ( 165 ) HTML (1 KB)  PDF (422 KB)  ( 26 )
160 Practice and consideration of hospital propaganda performance management
WANG Lei DU Mengkai ZHANG Guojun ZHANG Wei
Taking Xuanwu Hospital of Capital Medical University as an example, the concepts and methods of performance management are applied in hospital propaganda management. The significance, objectives and principles of hospital publicity performance management are clarified from four dimensions including publicity management, publicity workload, we-media construction and public opinion management. A hospital publicity performance evaluation index system covering 20 indicators at 2 levels was constructed. It provides a powerful starting point for the fine management of hospital publicity, forms a large pattern of hospital publicity from horizontal to bottom and vertical to the edge, and forms a closed loop system of continuous optimization of hospital publicity. This paper puts forward the ways and methods to improve the efficiency, quality and effect of hospital propaganda in the new period.
2019 Vol. 16 (31): 160-163,172 [Abstract] ( 214 ) HTML (1 KB)  PDF (796 KB)  ( 94 )
164 Rsearch on the operation practice of “enterprise custody medical care” in public hospitals
LI Xiang1 ZHONG Xumin2 XIE Jiewen2 LI Wenyuan1
At present, there are many researches on the Combination of medical treatment and maintenance in China, which is generally based on the micro level of society or policy. For the Combination of medical treatment and maintenance or operation model less mentioned. This paper is mainly aimed at the lack of motivation of service subjects in domestic public hospitals, Social capital into the lack of confidence, General operating problems. This paper makes an in-depth study on the specific operation and adopt the specific operation management of “enterprise custody medical care” of the Guangdong Second Traditional Chinese Medicine Hospital of  participating in the project of Combination of medical treatment and maintenance. It is expected to explore the scientific operation mode for public hospitals to carry out the erplicable service project of “Combination of medical treatment and maintenance”. It provides a reference for the theoretical research on the combination of medical treatment and maintenance in public hospitals with the participation of social capital.
2019 Vol. 16 (31): 164-168 [Abstract] ( 204 ) HTML (1 KB)  PDF (652 KB)  ( 83 )
169 Clinical effect of calcium alginate wound dressing on the prevention of complications at the PICC puncture site in patients with lung cancer
WAN Guangming1 WANG Yi1 PAN Luyi1 CHEN Pei2 ZHONG Hua1
Objective To investigate the clinical effect of calcium alginate wound dressing on preventing complications of puncture site of peripherally inserted central catheter (PICC) in patients with lung cancer. Methods A total of 557 patients with lung cancer who received PICC in the Chest Hospital Affiliated to Shanghai Jiao Tong University from January to December 2018 were selected as the study subjects. According to random number table method, the patients were divided into the observation group (n = 278) and the control group (n = 279). The observation group was treated with calcium alginate wound dressing nursing method, while the control group was treated with traditional nursing method. The difference of incidence and degree of complications occurred at the early stage (d≤7) and the number of cases and degree of complications after catheterization, PICC indwelling time, average daily cost, etc were analyzed between the two groups. Results The number of cases of early complications in the observation group was significantly lower than that in the control group, and the difference was statistically significant (P ＜ 0.05). The number of cases of skin injury related to puncture site in the observation group were less than those in the control group, and the difference was statistically significant (P ＜ 0.05). In addition, the observation group was superior to the control group in terms of postoperative pain, adhesion, healing time and scar size (P ＜ 0.05). Compared with the control group, the PICC retention time in the observation group was longer, the average daily cost was less, and the nursing satisfaction was higher (P ＜ 0.05). Conclusion The application of calcium alginate wound dressing after PICC catheterization can significantly reduce the incidence of early and late complications at the puncture site of lung cancer patients, while prolong the duration of PICC indwoundage, and improve postoperative recovery, which is worthy of clinical promotion.
2019 Vol. 16 (31): 169-172 [Abstract] ( 155 ) HTML (1 KB)  PDF (545 KB)  ( 69 )
173 Application of gradient elastic socks standard procedure in deep venous thrombosis of lower extremities after transurethral resection of prostate
MI Jiaxi LI Ping▲ ZHUANG Junlong
Objective To investigate the preventive effect of standard operating procedure of gradient elastic stockings on deep venous thrombosis of lower extremity after transurethral resection of prostate. Methods A total of 168 patients with transurethral resection of prostate from January 2016 to December 2018 were selected from Affiliated Drum Tower Hospital of Medicine School of Nanjing University, and randomly divided into observation group and control group with 84 patients in each group. Gradient elastic stockings were given to the control group, and the observation group was given the standard operating procedure of the gradient elastic stockings. The incidence of deep venous thrombosis and gastrocnemius pain, D-dimer level, lower limb blood flow velocity and lower limb skin temperature were compared between the two groups. Results The incidence of lower limb deep venous thrombosis and gastrocnemius pain in the observation group were significantly lower than those in the control group, with statistically significant differences (P ＜ 0.05). The postoperative D-dimer level in the observation group was lower than that in the control group, with statistically significant difference (P ＜ 0.05). The flow velocity of superficial femoral vein and popliteal vein of both lower extremities in the observation group were higher than those in the control group, and the skin temperature of both lower extremities in the observation group was higher than that in the control group, with statistically significant differences (P ＜ 0.05). Conclusion The standard operating procedure of gradient elastic socks can effectively prevent the occurrence of lower extremity deep vein thrombosis in patients undergoing transureal prostatectomy, reduce the incidence of gastrocrocker pain and D-dimer level, and improve the venous blood flow rate of lower extremity and skin temperature of lower extremity, which is worthy of promotion.
2019 Vol. 16 (31): 173-176 [Abstract] ( 173 ) HTML (1 KB)  PDF (651 KB)  ( 52 )
177 Application of individualized dietary guidance in hemodialysis patients with chronic renal failure
LIU Jing
Objective To investigate the application of individualized dietary guidance in patients with chronic renal failure on hemodialysis. Methods The clinical data of 110 patients with chronic renal failure in Yulin Second Hospital of Shaanxi Province from May 2015 to May 2018 were analyzed. According to the random number table method, the patients were divided into two groups, the control group (50 cases) received regular dietary guidance and the observation group (60 cases) received individualized dietary guidance. The simplified table of micro-nutrition evaluation (MNA-SF), quality of life score, serum albumin (ALB), serum prealbumin (PA), creatinine (Cr), nephrotic diet compliance scale (RAAQ) and behavior scale (RABQ) were observed before and after intervention. Results There was no significant difference in MNA-SF, RAAQ and RABQ between the two groups before intervention (all P ＞ 0.05). Comparison of quality of life score, PA and Cr between the two groups before and after intervention showed no statistically significant difference (all P ＞ 0.05). Compared with before intervention, MNA-SF, ALB, RAAQ and RABQ in both groups were significantly improved after intervention, and MNA-SF, ALB, RAAQ and RABQ in the observation group were higher than those in the control group, with statistically significant differences (all P ＜ 0.05). Conclusion Individualized dietary guidance in patients with chronic renal failure hemodialysis can improve their nutritional status and indicators, improve their dietary compliance, and is worthy of clinical application.
2019 Vol. 16 (31): 177-180 [Abstract] ( 190 ) HTML (1 KB)  PDF (546 KB)  ( 23 )
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