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| Effect and mechanism of oleanolic acid on proliferation and apoptosis of lung adenocarcinoma A549 cells |
| LI Kairui1 LIU Jie1 LI Xiaona1 HE Yingchun2,3,4 XU Zhaojun5,6 SONG Lan2,3,4 |
1.School of Postgraduate, Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410208, China;
2.School of Medicine, Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410208;
3.Hu′nan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hu′nan Province, Changsha 410208, China;
4.Hu′nan Provincial Ophthalmology and Otolaryngology Diseases Prevention and Treatment with Traditional Chinese Medicine and Visual Function Protection Engineering and Technological Research Center, Hu′nan Province, Changsha 410208, China;
5.Department of Cardiothoracic Surgery, the First Affiliated Hospital of Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410000, China;
6.the Domestic First-class Discipline Construction Project of Chinese Medicine of Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410000, China |
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Abstract Objective To investigate the effects of oleanolic acid on proliferation and apoptosis of lung adenocarcinoma A549 cells, and to explore its mechanism. Methods A549 cells were divided into control group (DMSO), different concentrations of oleanolic acid group (0.625, 1.25, 2.5, 5, 10 μmol/L) and positive control group (Cisplatin, 0.004 g/L) according to different culture methods. MTT and real-time cellular analysis technology (RTCA) were used to detect cell proliferation. Hoechst 33342 staining, mitochondrial membrane potential test, Annexin V-FITC/PI double fluorescent staining were used to detect cell apoptosis. Western blot was used to detect protein expression. Results MTT assay showed that the proliferation rate of A549 cells treated with oleanolic acid at different concentrations for 24, 48 and 72 hours was lower than that of the control group (P < 0.01). The results of RTCA showed that oleanolic acid could inhibit the proliferation of A549 cells. The results of Hoechst 33342 staining showed that compared with the control group, the fluorescence intensity of each group increased significantly (P < 0.05 or P < 0.01). The results of JC-1 staining showed that compared with control group, the red fluorescence intensity decreased significantly and the green fluorescence intensity increased significantly (P < 0.05 or P < 0.01). Annexin V-FITC/PI double fluorescent staining results showed that compared with the control group, the apoptosis of the treated groups increased significantly (P < 0.05 or P < 0.01). Oleanolic acid can down regulate the expression of anti apoptotic protein XIAP, survivin, Bcl-2, up regulate the expression of apoptotic protein Bax, cleaved casepase 3, cleaved caspase 8. Compared with control group, the difference was statistically significant (P < 0.05 or P < 0.01). Conclusion Oleanolic acid can inhibit the proliferation and induce apoptosis of lung adenocarcinoma cells, and its induction of apoptosis may be through the death receptor pathway or the mitochondrial pathway.
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