脓肿分枝杆菌临床分离株抗生素敏感性与基因型的关系

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Abstract Objective To observe the relationship between antibiotic susceptibility and genotype of clinical isolates of Mycobacterium abscessus. Methods From January 2016 to September 2018, 87 strains of Mycobacterium abscess isolated from Shanghai Pulmonary Hospital were selected for the study. Among them, 72 strains were sputum and 15 strains were bronchoalveolar lavage fluid. All strains were identified as Nontuberculous mycobacteria by MGIT960 medium growth and p-nitrobenzoic acid test, while further identified as Mycobacterium abscessus by sequencing rPOB gene. All strains were subjected to quantitative polymerase chain reaction amplification, while single nucleotide polymorphism analysis was performed using NCBI Nucleotide blast program. Antibiotic sensitivity was determined by microdilution method, while the relationship between antibiotic sensitivity and genotype was analyzed. Results Eighty-seven isolates included 81 subsp.abscessus, 6 subsp.massiliense. A total of 72 ERM (41) T28 sequences and 9 ERM (41) C28 sequences in 81 subsp.abscessus, and ERM (41) sequences to 10 species were sensitivity to antibiotics. 81 subsp.abscessu subtypes included 41 Claroycin (CLA) -sensitive strains and 10 CLA-mediated strains, 8 ERM (41) C28 and 43 ERM (41) T28 isolates without rrl 2058/2059 mutation. 30 resistant isolates were consisted of 29 ERM (41) T28 isolates (5 of them had rrl 2058/2059 mutations) and 1 ERM (41) C28 isolate (1 occurred the rrl 2058/2059 mutation). There were 2 subsp.massiliense resistant isolates, and 1 had a rrl 2058/2059 mutation. Conclusion The rrl and ERM (41) genotypes have a certain predictive effect on CLA resistance of clinical isolates of abscess abacillus. The treatment of Mycobacterium abscessus with Amikacin may have better effect and it is worthy of further study.

Key words： Mycobacterium abscessus Isolated strains Genotype Antibiotics Sensitivity

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MAO Yanhua CHU Haiqing. Relationship between antibiotic susceptibility and genotype of Mycobacterium abscessus clinical isolates[J]. 中国医药导报, 2019, 16(31): 94-97.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I31/94

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