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| Study of mitochondrial protein quality control in gastric smooth muscles of rats in spleen qi deficiency |
| XU Xinzhu1 LI Zhenyu2 LIU Wenjun3 WANG Yixuan1 CHEN Wenna4 SONG Nan5 SHAN Dehong6 |
1.Graduate School, Liaoning University of Traditional Chinese Medicine, Liaoning Province, Shenyang 110847, China;
2.Department of Nursing, Liaoning Vocational College of Medicine, Liaoning Province, Shenyang 110101, China;
3.Department of Functional Laboratory, College of Teaching Experiment Center, Liaoning University of Traditional Chinese Medicine, Liaoning Province, Shenyang 110847, China;
4.College of Medical Laboratory, Liaoning University of Traditional Chinese Medicine, Liaoning Province, Shenyang 110847, China;
5.Major Scientific Research Platform, Liaoning University of Traditional Chinese Medicine, Liaoning Province, Shenyang 110847, China;
6.Department of Physiology and Psychology, College of Basic Medicine, Liaoning University of Traditional Chinese Medicine, Liaoning Province, Shenyang 110847, China |
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Abstract Objective To explore the possible mechanism of stomach losing dropping in deficiency of spleen qi around mitochondrial protein quality control (MPQC) in gastric smooth muscles. Methods A total of 16 male SD rats were randomly divided into normal group and deficiency of spleen qi group (model group), with 8 rats in each group. The gastric residual rate was detected by charcoal powder propelling method, the ATP level in the gastric antrum smooth muscles(GASMs) was measured by ELISA, the mitochondrial structures in GASMs was observed by transmission electron microscopy, the expressions of hot shock protein 70 (Hsp70), Lon protease (LonP), caseinolytic protease (Clp)XP complex in GASMs were investigated by Western blot. Results Compared with the normal group, the gastric residual rate was greater (P ＜ 0.01), the ATP level was lower (P ＜ 0.05), the number of mitochondria was declined and some mitochondria became vacuolar, the expression of Hsp70 was unaltered, the expressions of LonP, ClpX and ClpP were all increased (P ＜ 0.01) in the model group. Conclusion The stomach losing dropping deficiency of spleen qi may be related to the decrease of mitochondrial productivity caused by MPQC abnormality of gastric antrum.
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