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| Effect of OPG-HSP70 on serological parameters of CIA mouse model and its correlation with efficacy |
| XIA Sisi1,2 ZHAO Wenming2 RONG Yujia2 |
1.Department of Rheumatology and Immunology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China;
2.Department of Immunology, School of Basic Medicine, Capital Medical University, Beijing 100069, China;
3.Laboratory Blood Bank, the 731 Hospital Affiliated to China Aerospace Science and Industry Group, Beijing 100074, China |
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Abstract Objective To investigate the relationship between serum parameters and disease status in collagen-induced arthritis (CIA) mice after OPG-HSP70 treatment. Methods Forty-four male DBA/1 mice were divided into OPG-HSP70 treatment group (n = 20), OPG treatment group (n = 12) and model control group (n = 12). CIA model was induced by intradermal immunization of bovine CⅡ in the tail root. OPG or OPG-HSP70 fusion protein was injected intraperitoneally every other day after arthritis, whereas model control group was injected with the same amount of PBS solution. The mice were sacrificed after 4 weeks of administration, the arthritis index was evaluated, and the relative gray value of bone was measured by Micro-CT. The serum was collected to detect Ca2+, RANKL and type Ⅱ collagen (CⅡ) antibodies. The linear regression was analysis between arthritis index and bone density values and serum data. Results Compared with the model control group, arthritis index of OPG-HSP70 treatment group was significantly reduced (P ＜ 0.01), the BMD value increased obviously (P ＜ 0.05), the CⅡ antibodies lower significantly (P ＜ 0.01). However, OPG did not significantly improve inflammation and bone damage. Linear regression analysis showed that, the serum Ca2+ and RANKL were negatively correlated with the bone density value (r = -0.582, -0.588, all P ＜ 0.01); while the serum CⅡ antibody level was positively correlated with the arthritis index (r = 0.892, P ＜ 0.01). Compared with the OPG treatment group, serum anti-OPG antibodies were significantly lower in the OPG-HSP70 treatment group 1, 2, 3, 4 weeks after administration (P ＜ 0.01). Conclusion The OPG-HSP70 fusion protein adds anti-inflammatory effect and reduces immunogenicity, and the protection of CIA mice is better than that of OPG.
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