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| Study on the curative effect and the best concentration of Galium verum L. Solution on chronic skin ulcer in diabetic rats |
| YUAN Haigang1,2 ZHAO Gang2 JIA Zhen2 LYU Bochuan2 HUANG Yanhong2 ZHANG Bailiang2 JIANG Deyou2 WANG Xuejun1 |
1.Heilongjiang Academy of Traditional Chinese Medicine, Heilongjiang Province, Harbin 150036, China;
2.Department of Peripheral Vascular Disease, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China |
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Abstract Objective To explore the curative effect and the best therapeutic concentration of Galium verum L. Solution on chronic skin ulcer in diabetic rats. Methods Sixty healthy male Wistar rats were selected to establish diabetic model by intraperitoneal injection of streptozotocin. Random number table method was used to divided the Wistar rats into six groups: high concentration group (30 mg/mL), medium concentration group (3 mg/mL), low concentration group (0.3 mg/mL), negative control group, model control group and Befuji group, 10 per group. Treatment was taken on the second day after modeling. On the basis of routine dressing change on the wound, the treatment groups of the scutellaria chinensis were sprayed with the corresponding concentration of Galium verum L. Solution. The negative control group was sprayed with normal saline, while the Befuji group was sprayed with Befuji (concentration was 4200 U/mL). The transparent membrane was used to tracing film along the ulcer edge and the software was taken to calculate the remaining area on the third, seventh and tenth day after modeling. All the rats were sacrificed and local wound tissues were stained with HE to observe the pathological changes on the tenth day after modeling. Results The ulcer surface of each group decreased gradually with time. The wound areas of various concentrations of Galium verum L. groups and the Beifuji group were significantly smaller than those of the negative control group and the model control group at the same time point, and the differences were statistically significant (P ＜ 0.05). The wound area of various concentrations of Galium verum L. groups were significantly smaller than those of the Beifuji group at the same time point, while the wound area of medium concentration Galium verum L. group decreased most obviously, and the differences were statistically significant (P ＜ 0.05). There are interactions between different processing factors and time factors (P ＜ 0.05). The wound repair of beifuji group was better than that of negative control group and model control group, and each treatment group with different concentration of Galium verum L. Solution were superior to the Befuji group, while the wound healing and repair of the medium concentration group was the best, and the differences were statistically significant (P ＜ 0.05). Conclusion The Galium verum L. Solution is more effective than Beifuji in the treatment of skin ulcer in rats at the different points. And the therapeutic optimal concentration of Galium verum L. Solution in promoting wound healing in rats was 3 mg/mL.
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