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2018 Vol. 15, No. 32
Published: 2018-11-15
 
 
4 Effect of vaccum sealing drainage in C57BL/6J mice with burn sepsis
LIU Yang BAI Xiaozhi LI Xiaoqiang CHEN Ziyue HE Ting▲
Objective To investigate the effect of vaccum sealing drainage (VSD) on C57BL/6J mice with burn sepsis. Methods A total of 300 male C57BL/6J mice were used to establish the model of burn sepsis. Eighty burned sepsis mice successfully were divided into experimental group and control group according to random number table, 40 mice in each group. In the experimental group, the wound was disposed and treated with VSD, the negative pressure apparatus was connected, and the negative pressure was adjusted to -120 mmHg (1 mmHg=0.133 kPa). In the control group, only wound treatment was performed, and dressing was replaced every 24 h. The fluorescence intensity and blood flow of wound skin, the levels of inflammatory cytokines such as IL-1β, IL-6, IL-8, IL-10, TNF-α and so on were statistically calculated in the two groups, and the cumulative survival rate of 7 d was observed. Results Before treatment, the fluorescence intensity of wound was similar between the two groups, and the difference was not statistically significant (P > 0.05). Compared with before treatment, the fluorescence intensity of the control group increased significantly after 24 h of treatment (P < 0.05), while the fluorescence intensity of the experimental group decreased compared with before treatment (P < 0.05). The difference between the two groups was statistically significant (P < 0.05). Compared with before treatment, the control group showed a decrease on the 1st and 2nd day after treatment, and an increase on the 3rd, 5th and 7th day after treatment, there were statistically significant differences (P < 0.05 or P < 0.01). The blood flow to the wounds on the back of the experimental group gradually increased and were significantly higher than those of the control group after treatment (P < 0.05 or P < 0.01). In the experimental group, the contents of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α were significantly reduced and IL-8 was significantly increased compared with the control group (all P < 0.05), while the changes of IL-10 were not significant (all P > 0.05). From day 2, the survival rates of the experimental group were significantly higher than those of the control group, with statistically significant differences (all P < 0.05). Conclusion VSD treatment for C57BL/6J mice with burn sepsis can reduce bacteria content of wounds, improve blood supply, reduce systemic inflammatory response level of sepsis and improve survival rate.
2018 Vol. 15 (32): 4-8 [Abstract] ( 210 ) HTML (1 KB)  PDF (837 KB)  ( 71 )
9 Experimental animal study of injury and kidney pelvis line suture in Chinese rural dogs
LIU Gao1,2 FU Dian3 CHENG Wen3
Objective To investigate the effect of continuous suture of renal pelvis, renal parenchyma and renal capsule on wound suture after removal of renal pelvis tumor. Methods Four healthy male Chinese rural dogs were selected. After successful anesthesia, the operation was performed according to the human open kidney partial resection procedure. The sutures of the renal pelvis mucosa, submucosa, muscular layer and renal layer were sutured continuously. The operation time, intraoperative blood loss, arterial block time, postoperative recovery time, and perioperative complications were observed. Results The operation time of renal artery occlusion, local occlusion time, renal artery occlusion time and local occlusion time were (39.00±2.16), (37.50±4.30), (10.25±2.50), (8.45±1.81) min, respectively. The amount of bleeding in the renal artery block partial nephrectomy and suture was (54.50±4.21) mL, and the amount of hemorrhage during local partial nephrectomy and suturing was (57.50±9.58) mL. After the anesthesia waken up, they could move in place. The next day, they were given food and returned to normal after a week. Conclusion The operation time is short, the amount of bleeding is small, the recovery is fast, and there are no serious complications such as major bleeding and urinary leakage after operation. It effectively prevents and eliminates two common complications after partial nephrectomy, which lays a foundation for its clinical application.
2018 Vol. 15 (32): 9-12 [Abstract] ( 196 ) HTML (1 KB)  PDF (800 KB)  ( 68 )
13 Cloning, expression, purification of human blood-brain barrier permeability related gene Caveolin-1
ZHAO Shutao XU Yongjian CHEN Haoyun
Objective To clone Caveolin-1, a gene related to human blood-brain barrier permeability, and express it in Escherichia coli. and to purify and identify the protein finally. Methods According to the sequence of Caveolin-1 gene contained in the gene bank, the upstream and downstream primers were designed. The Caveolin-1 double strand was synthesized by PCR and then recombined with pET28a (+) vector. Positive clones were screened and identified by DNA sequence analysis. The constructed recombinant plasmid pET28a-Caveolin-1 was transfected into E. coli BL21 (DE3), and the expression of the fusion protein was induced by IPTG. The expressed product was purified by Ni2+-NTA affinity chromatography, identified by SDS-PAGE and Western blot. Results The Caveolin-1 DNA fragment amplified by PCR was identified as human Caveolin-1 gene. The recombinant human Caveolin-1 fusion protein was expressed by IPTG-induced DE3 strain containing the recombinant plasmid pET28a-Caveolin-1. The recombinant protein was purified by Ni2+-NTA affinity chromatography to obtain a fusion protein of higher purity. Conclusion This study successfully cloned the blood-brain barrier permeability-related gene Caveolin-1, and expressed and purified the protein to obtain a higher purity fusion protein, which laid a foundation for further relevant clinical research.
2018 Vol. 15 (32): 13-15,23 [Abstract] ( 214 ) HTML (1 KB)  PDF (666 KB)  ( 103 )
16 Experimental study on seborrheic alopecia in animals of Sanshen Yangfa Sprays
SHI Xiaofang1 LI Jian1 ZHAO Shengxia1 ZHAO Zhangguang1 SUN Jianhui2 HUO Hairu2 LI Xiaoqin2 LI Hongmei2
Objective To investigate the therapeutic effect and anti-inflammatory effct of Sanshen Yangfa Sprays on the seborrheic alopecia animal model. Methods Sixty SPF male Wistar rats and sixty SPF male Institute of Cancer Research (ICR) mice were injected with testosterone propionate for 60 consecutive days, resulting in experimental seborrheic alopecia model. According to the weight block, the rats and mice were randomly divided into normal control group, model control group, high dose (0.290 g crude drug/mL), medium dose (0.145 g crude drug/mL), low dose (0.073 g crude drug/mL) of Sanshen Yangfa Sprays group, and Minoxidil Tincture group (0.05 g/mL), with 10 animals in each group, the length of hair was measured, the hair follicles and sebaceous glands were examinated by pathologic histology. According to the weight block, 50 SPF male C57BL/6 mice were randomly divided into blank control group, high dose (0.290 g crude drug/mL), medium dose (0.145 g crude drug/mL), low dose (0.073 g crude drug/mL) of Sanshen Yangfa Sprays group, and Minoxidil Tincture group (0.05 g/mL), with 10 mice in each group, the time of mouse hair entering the growth period from the rest period, the time from entering the growth period to growing the skin surface, and the length of hair growth were observed. The model of capillary permeability of mice induced by acetic acid and the model of mouse ear swelling induced by croton oil were used to research the anti-inflammatory effect of Sanshen Yangfa Sprays. According to weight block, 50 SPF male ICR mice were randomly divided into model control group, high dose(0.290 g crude drug/mL), medium dose (0.145 g crude drug/mL), low dose (0.073 g crude drug/mL) of Sanshen Yangfa Sprays group, and Yunnan Baiyao Tincture group, with 10 mice in each group, the absorbance of peritoneal fluid and value of ear swelling were measured. Results At the 15th, 30th, 45th, 60th day of drug administration, the hair length of rats in the high, medium and low dose of Sanshen Yangfa Sprays group were longer than those in the model control group (P < 0.05); the lesion degree of dermal tissue cells, hair follicles and sebaceous glands in rats of the high and medium dose of Sanshen Yangfa Sprays group were lower than those of the model control group (P < 0.05); the number of damaged hair follicles in the rats of the high and medium dose of Sanshen Yangfa Sprays group was lower than those of the model control group (P < 0.05). At the 15th, 30th and 45th day of administration, the hair length of the mice of the high and medium dose of Sanshen Yangfa Sprays group were longer than those of the model control group (P < 0.05); the number of damaged hair follicles in the mice of the high and medium dose of Sanshen Yangfa Sprays group were significantly lower than those of the model control group (P < 0.05); the lesion degree of dermal tissue cells, hair follicles and sebaceous glands in mice of the high dose of Sanshen Yangfa Sprays group was lower than that of the model control group (P < 0.05). The time of hair follicles from resting period to growing period, from entering growing period to growing surface of mice in high and medium dose of Sanshen Yangfa Sprays group were significantly shorter than those of blank control group (P < 0.05); the hair length was significantly higher than that of the blank control group at the 15th, 30th, 45th day of administration (P < 0.05). The absorbance of peritoneal fluid of mice in the high dose of Sanshen Yangfa Sprays group was significantly lower than that in the model control group (P < 0.05). The value of ear swelling of mice in the high and medium dose of Sanshen Yangfa Sprays group was significantly lower than that in the model control group (P < 0.01). Conclusion Sanshen Yangfa Sprays have the effect of promoting hair growth and anti-inflammatory effect on sebrrheic alopecia.
2018 Vol. 15 (32): 16-23 [Abstract] ( 185 ) HTML (1 KB)  PDF (2204 KB)  ( 229 )
24 Research progress on atherosclerosis promoted by endoplasmic reticulum stress of adipose tissue in children with obesity
WANG Mingxia JIA Lihong ZHAI Lingling WEI Wei SUN Qi BAI Yinglong
Cardiovascular disease is the most serious public health problem that currently threatens the health of people in our country. Atherosclerosis is the main pathological basis and can occur in childhood, and the risk of obesity in children is increasing. Endoplasmic reticulum stress is an important mechanism for the development of atherosclerosis. Macrophage infiltration in obese adipose tissue increases, endoplasmic reticulum stress aggravates, interaction between adipocytes and macrophages accelerates atherosclerotic progression, which plays an important role in the development of atherosclerotic cardiovascular disease. For deepening people′s knowledge of obesity accelerating atherosclerosis and raising awareness of preventing childhood obesity, and exploiting new ideas for treating atherosclerosis in children with obesity, this review introduced the relationship between endoplasmic reticulum stress of adipose tissue and the development of atherosclerosis in children with obesity.
2018 Vol. 15 (32): 24-27 [Abstract] ( 209 ) HTML (1 KB)  PDF (647 KB)  ( 66 )
28 Progress in treatment of femoral neck fracture in children
LYU Jing1 SUN Hanrui1 FAN Yanan1 WEN Yangyang1 LI Wenlong2 LIU Youwen3
Children′s femoral neck fracture is a disease with a low clinical incidence. With the continuous development of society, children with femoral neck fractures caused by high-energy injuries are increasing. If the disease is not effectively treated, it will seriously affect the development of hip joints in children. At present, there are many treatments for femoral neck fractures in children, but clinically there is no unified and effective treatment plan. In this paper, the anatomical characteristics, treatment methods and complications of children′s femoral neck are discussed. It focuses on the analysis of the choice of different types of fracture treatment options and the feasibility of 3D printing technology and the development of a new type of child-specific fixation device for this disease, and in order to provide ideas for the treatment and research of femoral neck fractures in children.
2018 Vol. 15 (32): 28-31 [Abstract] ( 177 ) HTML (1 KB)  PDF (694 KB)  ( 71 )
32 Advances in the study of neuromyelitis optic spectrum disease
WANG Chunchen1 CHEN Zhigang2▲ WANG Hong1 LI Bin1 XING Yi3 WANG Guiling1 LIU Chunhong4
Neuromyelitis optica is an inflammatory demyelinating disease of central nervous system involving the optic nerve and spinal cord. As a demyelinating disease of the central nervous system, it has high incidence in Asia. It has high recurrence rate, severe clinical symptoms and poor prognosis, which affect the life quality of patients seriously. Recently, it has become a hot topic in the study of neuroimmunology area. In this paper, we summarize and analyze the characteristics, epidemiology, pathophysiological basis, clinical manifestation, diagnostic evaluation, diagnostic criteria, differential diagnosis, acute stage treatment, prevention of attack treatment and prognosis of the disease, in order to provide ideas for clinical diagnosis and further scientific research.
2018 Vol. 15 (32): 32-37,49 [Abstract] ( 232 ) HTML (1 KB)  PDF (694 KB)  ( 136 )
38 Progress of traditional Chinese medicine and Western medicine treatment of uterine prolapse
PAN Dingchen1 YANG Birong2 MA Qingliang2 JIANG Guojing2
Uterine prolapse is also called Yinting, Yinjun or Yintuo in Chinese medicine. Yizong Jinjian proposed "if women suffered from uterine prolapse, it maybe due to uterine collaterals′ damage or delivery forcibly or collapse of qi or damp-heat in lower jiao". So there are three kinds of TCM syndromes, collapse of qi, collapse of kidney′s qi and damp-heat in lower jiao. With the trend of urbanization, the incidence of this disease for aged patients has increased year by year, which has caused great damage to the daily life of women. Therefore, uterine prolapse should be paid more attention. Since ancient times, traditional Chinese medicine has had an indispensable advantage in this field. With the rapid development of modern medicine, the cure rate of uterine prolapse has been improved significantly. This paper will classify the progress of traditional Chinese medicine and Western medicine treatment in uterine prolapse, including oral Chinese medicine, fumigation of traditional Chinese medicine, acupuncture, electro-acupuncture, moxibustion, acupoint injection, acupoint embedding, modern surgical treatment, and non-surgical treatment etc. We hope to help the development of Chinese medicine and modern medicine in this field.
2018 Vol. 15 (32): 38-41 [Abstract] ( 185 ) HTML (1 KB)  PDF (693 KB)  ( 202 )
42 Advances in pain assessment tools for elderly cancer patients
CUI Shuang1 GUO Renxiu2 ZHENG Ying3▲
This article searches the databases of Web of Science, PubMed, CNKI, Viper,Wanfang debases, literature is looked up, to look for the existing pain assessment tools for elderly cancer patients. The existing pain assessment scales for elderly cancer patients are divided into 5 self-descriptive assessment scales. VDS assessment is preferred for the elderly with severe cognitive impairment or mild or moderate cognitive impairment. There are 6 objective pain observation scales, and four scales of which are recommended to be used first, but not widely. Objective evaluation of elderly patients with pain has not been paid much attention. Foreign scales are not widely used in China. Attention should be paid to the pain evaluation of elderly cancer patients.
2018 Vol. 15 (32): 42-45 [Abstract] ( 230 ) HTML (1 KB)  PDF (649 KB)  ( 122 )
46 Research progress of the correlation of thyroid and breast diseases
ZHAO Yingjie1 ZHANG Hehuan2
The incidence of thyroid and breast diseases is increasing， thyroid and breast belong to hormone response organ. There is a clear correlation between the two diseases， thyroid disease can increase the risk of breast disease and vice versa. The same pathogenesis of them is still under study. It is clear that estrogen and thyroxine promote the occurrence and development. This paper reviews the progress of researchs concerning the role of estrogen and thyroid hormone in thyroid and breast diseases.
2018 Vol. 15 (32): 46-49 [Abstract] ( 280 ) HTML (1 KB)  PDF (647 KB)  ( 54 )
50 Advances in studies on pharmacological effect of arctigenin
WANG Zhe WANG Jiahe
Arctigenin (ARC-G) is an effective active ingredient of traditional Chinese medicine Arctii Fructus. In recent years, it has been confirmed by scholars that it has a variety of pharmacological effects, such as anti-inflammatory, immunomodulatory, anti-virus, anti-tumor activity, and it also has a certain effect on diabetic nephropathy and some neurodegenerative diseases. This article reviews a large number of domestic and foreign literatures in recent years, systematically analyzes the various pharmacological effects and mechanisms of action of ARC-G, highlights the influence of ARC-G on tumor cells, provides a theoretical basis for the further studies and clinical application of ARC-G.
2018 Vol. 15 (32): 50-53 [Abstract] ( 283 ) HTML (1 KB)  PDF (645 KB)  ( 124 )
54 Study on the cognition, attitude and influencing factors of painless induced abortion in college students
XIE Min1 ZHAO Miao2
Objective To understand the college students′ cognition and attitude of painless induced abortion and the influencing factors. Methods Chengdu Medical College and Southwest Petroleum University were chosen as the survey sites. The questionnaire survey was conducted with stratified cluster random sampling method from March 6 to 15, 2017. The survey data were analyzed by SPSS 17.0. Results A total of 693 questionnaires were distributed, 664 valid questionnaires were obtained after the invalid questionnaires were removed. The efficiency was 95.8%. Only 85 of the respondents (12.8%) had contacted the information of painless induced abortion initiatively. In the aspect of contacting the information of painless induced abortion initiatively, the proportion of students in different age groups and different grades had a statistically significant difference (P ＜ 0.05). The main route for respondents to contact the information of painless induced abortion was advertising (100%). Respondents were less aware of painless induced abortion, the dominant situation was "very little understanding", accounting for 64.2%. Respondents′ main attitude towards painlessness was "neutral", accounting for 62.7%. The scores of basic test about painless induced abortion were very low, the average score of the whole test was (1.05±1.28) scores. The cognition level of painless induced abortion was positively correlated with grade, and negatively related to gender (female as reference) (P ＜ 0.05). Conclusion College students lack the knowledge of painless induced abortion, and students′ reproductive health education should be done well in colleges and universities.
2018 Vol. 15 (32): 54-57 [Abstract] ( 276 ) HTML (1 KB)  PDF (362 KB)  ( 61 )
58 Correlation between heart rate variability and the severity of coronary artery disease evaluated by Syntax score in patients with primary hypertension
LI Ke1,2 LIU Jianxiong2 LIANG Wei2 LIN Lanlan2 YANG Shijian2 WANG Xin2
Objective To investigate the relationship between severity of coronary artery disease and heart rate variability (HRV) in patients with primary hypertension. Methods From January 2016 to December 2017, 307 patients who were diagnosed as primary hypertension in Chengdu Second People′s Hospital Affiliated to Zunyi Medical University and underwent coronary angiography (CAG) were enrolled in this study. According to the principle of CAG diagnosis of coronary heart disease (CHD), the samples were divided into non-CHD group (121 cases) and CHD group (186 cases), the general clinical data, the biochemical indexes of serology and the 24 h dynamic electrocardiogram of the two groups were compared. Syntax score system was used to evaluate the severity of coronary artery disease, and the CHD group was divided into severe group (53 cases), moderate group (64 cases) and mild group (69 cases) according to the Syntax score. The differences on HRV of the three groups and the relationship between the severity of coronary artery disease and HRV was analyzed. Results The ratio of male, smoking, high cholesterol history, age and high density lipoprotein cholesterol levels of the non-CHD group were lower than those of the CHD group, the differences were statistically significant (P < 0.05). There were no statisticaly significant differences on body mass index, drinking alcohol, history of hyperuricemia, uric acid, total cholesterol, triglyceride and low density lipoprotein cholesterol between non-LDH group and LDH group (P > 0.05). Multivariate two element Logistic regression analysis showed that smoking, age and hyperlipidemia were related to CHD in patients with primary hypertension, the differences were statistically significant (P < 0.05). When comparing the HRV indexes [standard diviation of NN intervals (SDNN), standard deviation of the mean of all 5 minutes segments of normal RR intervals (SDANN), root mean square successive difference between adjacent normal RR intervals (rMSSD), percent of difference between adjacent normal RR intervals that was longer than or equal to 50 ms (PNN50) and low frequency (LF), high frequency (HF), LF/HF] in each group, the indexes of non-CHD group were significantly higher than those of the other groups (P < 0.05), the indexes of the mild group were significantly higher than those of the moderate group and severe group, and the indexes of the moderate group were significantly higher than those of the severe group, the differences were statistically significant (P < 0.05). The time domain and frequency domain indicators in HRV were negatively correlated with the severity of coronary artery disease in patients with CHD (SDNN: r = -0.52, P = 0.000; SDANN: r = -0.50, P = 0.007; rMSSD: r = -0.41, P = 0.018; PNN50: r = -0.45, P = 0.017; LF: r = -0.67, P = 0.009; HF: r = -0.58, P = 0.021). Conclusion Age, smoking and history of hyperlipidemia are the risk factors of CHD in hypertensive patients. The HRV is negatively correlated with severity of coronary artery disease. HRV index can be used to evaluate the severity of coronary artery disease in patients with hypertension.
2018 Vol. 15 (32): 58-62 [Abstract] ( 258 ) HTML (1 KB)  PDF (485 KB)  ( 77 )
63 Correlation analysis of N-terminal pro-brain natriurctic peptide and left atrial volume with recurrence after radiofrequency catheter ablation of atrial fibrillation
GUO Fei XU Jian SU Hao ZHU Hongjun CHEN Kangyu ZHU Jing
Objective To investigate the correlation of N-terminal pro-brain natriurctic peptide (NT-proBNP) and left atrial volume (LAV) with the recurrence after atrial fibrillation catheter radiofrequency ablation in patients with atrial fibrillation. Methods One hundred and six patients with paroxysmal atrial fibrillation treated in First Affiliated Hospital of University of Science and Technology of China from May 2017 to January 2018 were selected as the research subjects, all patients were performed radiofrequency ablation of atrial fibrillation catheter. The plasma NT-proBNP level before operation was detected by chemiluminescent immunoassay, LAV of the patients was measured by transesophageal echocardiography. Three months was followed-up after operation, according to whether recurrence occurred, the patients were divided into recurrence group and non-recurrence group. Logistic multivariate regression analysis was used to analyze the risk factors for recurrence after radiofrequency catheter ablation of atrial fibrillation. Results Follow-up observation showed that 16 cases recurred after operation, and the recurrence rate was 14.68%. The plasma NT-proBNP and LAV of the recurrence group were higher than those of the non-recurrence group, the differences were statistically significant (P < 0.05). Multivariate Logistic regression analysis showed that plasma NT-proBNP and LAV were independent risk factors for recurrence after radiofrequency catheter ablation of paroxysmal atrial fibrillation (P < 0.05). Conclusion The levels of plasma NT-proBNP and LAV before operation in patients with recurrent atrial fibrillation after radiofrequency catheter ablation are independent risk factors for recurrence after operation, detection of plasma NT-proBNP and LAV before operation can help predict the recurrence of patients after operation.
2018 Vol. 15 (32): 63-66 [Abstract] ( 282 ) HTML (1 KB)  PDF (503 KB)  ( 108 )
67 The value of NT-proBNP and P wave dispersion in the diagnosis and prognosis of patients with paroxysmal atrial fibrillation
YAN Chao1 WANG Ling2
Objective To analyse the changes of N-terminal pro-brain natriuretic peptide (NT-proBNP) level and P wave dispersion (Pd) in patients with paroxysmal atrial fibrillation (PAF), and provide the reference for the diagnosis and prognosis of PAF. Methods One hundred and thirty PAF patients in Inner Mogolia People′s Hospital were selected as the atrial fibrillation group and 50 healthy physical examination people were selected as control group. The changes of NT-proBNP level and Pd value of the two groups before and after cardioversion were analyzed, patients in the atrial fibrillation group were followed up for 6 months after discharge. The relationship between the NT-proBNP level and the Pd value before and after the cardioversion of atrial fibrillation was analyzed. Results The level of NT-proBNP before cardioversion in the atrial fibrillation group was significantly higher than that in the control group (P < 0.05); the level of NT-proBNP after cardioversion in the atrial fibrillation group was lower than that before cardioversion, which was still higher than that in the control group, the differences were statistically significant (P < 0.05). The Pd value before cardioversion in the atrial fibrillation group was significantly higher than that in the control group (P < 0.05); the Pd value after cardioversion in the atrial fibrillation group was lower than that before cardioversion (P < 0.05), but it had no significant difference compared with that in the control group (P > 0.05). After diacharge for 3 months, the level of NT-proBNP before cardioversion in the recurrent PAF group was higher than that in the non recurrent PAF group before cure, the difference was statistically significant (P < 0.05). After discharge for 3, 4, 5, 6 months, the level of NT-proBNP after cardioversion in the recurrent PAF group was higher than that in the non recurrent PAF group after cardioversion, the differences were statistically significant (P < 0.05). Conclusion The NT-proBNP level and Pd value have good application value for the diagnosis and prognosis of patients with PAF.
2018 Vol. 15 (32): 67-70 [Abstract] ( 223 ) HTML (1 KB)  PDF (459 KB)  ( 108 )
71 The effect of mirror therapy combined with electromyographic biofeedback on lower extremity motor function and balance function of subacute ischemic stroke patients.
XU Leyi SUO Guanghu LIN Zuchen JIN Haimin WANG Zhewei
 Objective To observe the effective of mirror therapy combined with electromyographic biofeedback on lower extremity motor function and balance function and activities of daily living of subacute ischemic stroke patients. Methods From November 2016 to March 2018， 29 cases of subacute ischemic stroke patients in the Department of Rehabilitation Medicine, the First Affiliated Hospital of Wenzhou Medical University were selected, and they were divided into mirror therapy combined with electromyographic biofeedback group (MT+EMGBF, 14 cases） and electromyographic biofeedback group (EMGBF, 15 cases）. The two groups were treated with conventional methods. On the basis of the former treatment, EMGBF group was given knee extension and ankle flexion training with myoelectric biofeedback therapy apparatus, and MT+EMGBF group was given the above training by using lower limb mirror treatment combined with myoelectric biofeedback therapy apparatus. The treatment sustained for 30 min， 1/d， 5 d/week， and the course was 8 weeks. Fugl-Meyer assessment lower extremity（FMA-LE）， Berg balance score（BBS） and modified Barthel index（MBI） was adopted to evaluate the motor function， balance function and activity of daily living, respectively. Results After treatment, scores of FMA-LE， BBS and MBI of the two groups had improved than those before treatment （P ＜ 0.01）. Compared with the EMGBF group, motor function and balance function in the MT+EMGBF group improved significantly (P ＜ 0.05), while there was no significant difference between the two groups in the improvement of activity of daily living （P ＞ 0.05）. Conclusion In the early stage, electromyographic biofeedback can significantly improve the motor function of the lower extremity, balance function and ability of daily living in subacute ischemic stroke patients. On that basis, electromyographic biofeedback combined with mirror therapy has more significant improvement in motor function and balance function in the lower extremity.
2018 Vol. 15 (32): 71-74 [Abstract] ( 242 ) HTML (1 KB)  PDF (389 KB)  ( 67 )
75 Effect of Shenqi Tiaoti Decoction combined with Western medicine in the treatment of polycystic ovarian syndrome with spleen deficiency and phlegmatic hygrosis
DING Chunli1 XU Fang2 LI Yan3 HOU Lihui2▲
Objective To observe the clinical effect of Shenqi Tiaoti Decoction combined with Western medicine in the treatment of polycystic ovarian syndrome with spleen deficiency and phlegmatic hygrosis. Methods A total of 86 polycystic ovarian syndrome patients with spleen deficiency and phlegmatic hygrosis, who cured in Outpatient Service of Gynaecology Department in the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine from June 2014 to March 2017, were divided into control group and observation group according to registration order, with 43 cases in each group. The control group was given lifestyle intervention and drug therapy, while the observation group was given Shenqi Tiaoti Decoction on the base of control group. The TCM symptom score, serum hormone, insulin resistance and ovary indexes of two groups before and after treatment were recorded. And the total effective rates of two groups were calculated. Results After treatment, the symptom scores delayed menorrhea, nausea phlegm, mentally fatigued and tongue thick and greasy of two groups were all obviously lower than before, and those in observation group were all lower than control group, the differences were statistically significant (P < 0.05). After treatment, the testosterone, estradiol, luteinizing hormone, follicle stimulating hormone, fasting blood glucose, fasting insulin and insulin resistance index of two groups were all obviously lower than before, and those in observation group were all obviously lower than control group, the differences were statistically significant (P < 0.05). After treatment, the ovarian volume and number of basal sinus follicles of two groups were both obviously less than before, and the ovarian volume, number of basal sinus follicles in observation group were lower than control group, the mature follicles in observation group was more than control group, the differences were statistically significant (P < 0.05). The total effective rate of clinical treatment in observation group was obviously higher than control group, the difference was statistically significant (P < 0.05). Conclusion Shenqi Tiaoti Decoction combined with Western medicine has curative effect in the treatment of polycystic ovarian syndrome with spleen deficiency and phlegmatic hygrosis, it can effectively improve symptoms of spleen deficiency and phlegmatic hygrosis, and regulate endocrine disorders and blood sugar metabolism, promote the recovery of ovarian function.
2018 Vol. 15 (32): 75-79 [Abstract] ( 217 ) HTML (1 KB)  PDF (577 KB)  ( 88 )
80 Study on high-risk HPV E6 / E7 mRNA combined with liquid - based cytology to improve the detection rate of cervical lesions
MA Na ZHANG Yan▲ QIAO Na
Objective To study the application value of the high-risk human papillomavirus (HPV) E6/E7 mRNA combined with liquid based cytology in the improvement of detection rate of cervical lesion. Methods From January 2016 to December 2017, 249 patients with cervical lesions in Yan′an People′s Hospital were selected as the subjects. There were 170 cases of cervical inflammation, 32 cases of cervical intraepithelial neoplasia Ⅰ (CIN Ⅰ), 28 cases of CIN Ⅱ, 14 cases of CIN Ⅲ, 5 cases of cervical cancer. The detection of high-risk HPV E6/E7 mRNA, liquid based cytology and histopathological biopsy were carried among all patients. The detection rate, sensitivity, and specificity of different examination methods were compared with the results of pathological biopsy. Results The detection rate of high risk type HPV E6/E7 mRNA examination in the diagnosis of cervical inflammation, CIN was 83.53%, 71.62%, which was significantly higher than liquid based cytology of 68.24%, 56.76%, and was significantly lower than the joint detection of 94.71% and 90.54% respectively, the differences between groups were statistically significant (P < 0.05). The detection rate of high-risk type HPV E6/E7 mRNA examination in the diagnosis of CIN Ⅰwas 68.75%, which was significantly higher than the liquid based cytology of 43.75%, the joint detection was significantly lower than the 90.63%, the differences between groups were statistically significant (P < 0.05). The sensitivity of the high-risk HPV E6/E7 mRNA examination and the diagnosis of precancerous lesions was 76.60% and 68.09% respectively, which was significantly lower than 91.49% of the joint detection. The specificity of high-risk HPV E6/E7 mRNA in diagnosing precancerous lesions was 81.19%, which was significantly higher than the liquid based cytology of 64.36%, was significantly lower than the joint detection of 94.06%, the differences between groups were statistically significant (P < 0.05). Conclusion The high risk HPV E6/E7 mRNA combined with cytological examination can significantly improve the detection rate of cervical lesions, which has higher clinical diagnostic value, and worth popularizing.
2018 Vol. 15 (32): 80-83 [Abstract] ( 196 ) HTML (1 KB)  PDF (577 KB)  ( 42 )
84 Analysis on pathogen distribution and immunological monitoring results of recurrent respiratory infection in 3-6 years old children in Tongzhou District
LIU Wei WU Hongbo
Objective To analyze the pathogen distribution and immunological monitoring results of recurrent respiratory infection in 3-6 years old children in Tongzhou District. Methods A total of 135 children with recurrent respiratory infection admitted to Beijing Luhe Hospital, Capital Medical University ("our hospital" for short) from March 2015 to July 2017 were selected as the observation group, the samples of children′s secretions were extracted and pathogenic bacteria were cultured and identified, and the distribution of pathogenic bacteria was analyzed, another 87 healthy children carried physical examination in our hospital in the same period were selected as control group, the humoral immunity index, cellular immunity index and inflammatory cytokine level were compared between the two groups. Results Ninety-four pathogenic bacteria were detected in the observation group, Gram-positive bacteria accounted for 27.66%, Gram-negative bacteria accounted for 72.34%, detection rate of Gram-positive bacteria was significantly lower than that of Gram-negative bacteria(P < 0.01); there was no significant difference in immunoglobulin A (IgA) and C4 between the two groups (P > 0.05), the immunoglobulin M (IgM), immunoglobulin G (IgG) and C3 in observation group were significantly lower than those in control group (P < 0.01); there was no significant difference in CD4+ between the two groups (P > 0.05), the CD3+ and CD4+/CD8+ in observation group were significantly lower than those in control group, and the CD8+ was significantly higher than control group (P < 0.01); the interleukin-4 (IL-4), IL-6 and IL-10 in the observation group were significantly higher than those in the control group, the IL-12 was significantly lower than the control group (P < 0.01). Conclusion 3-6 years old children with recurrent respiratory tract infection have low immunologic function, Gram-negative bacteria are the main pathogens of infection, according to the detection results of pathogenic bacteria and immunologic function, appropriate antibiotics or immunologic enhancers should be selected to treat the children and improve their prognosis.
2018 Vol. 15 (32): 84-87 [Abstract] ( 213 ) HTML (1 KB)  PDF (530 KB)  ( 92 )
88 Diagnostic value of egg component protein, galactose agglutinin-3 and allergen IgE antibody applied in the diagnosis of children with egg allergy
TANG Chuanting
Objective To investigate the value of combined detection of egg component protein, galactose agglutinin-3 and allergen IgE antibody in the diagnosis of egg allergy. Methods Forty-seven patients with food allergy who were screened in West China-Guang′an Hospital, Sichuan University from February 2017 to April 2018 were selected as study group. Another 20 patients without allergie history and negative results venous blood test and egg serum-specific IgE were selected as the control group. Serum egg component protein, galactose agglutinin-3 and allergen IgE antibody were detected in two groups. Results According to anaphase follow-up, there were 42 cases of egg allergy and the others were misdiagnosed in 47 suspected children with egg allergy. The levels of sIgE and total IgE in the study group were higher than those in the control group, the differences were statistically significant (P < 0.05). In the study group, the positive rates of IgE and inhales were 91.49% and 82.98%, respectively. The positive rates of ingestion and inhaled IgE were 70.21%. The OD values of ovalbumin, ovomucoid, otransferrin and lysozyme in the study group were significantly lower than those in the control group, the differences were statistically significant (P < 0.05), and the OD value of the control group was close to the blank hole. The level of galactose agglutinin-3 in the study group was significantly higher than that in the control group, the difference was statistically significant (P < 0.05). The sensitivity, accuracy and specificity of egg component protein in the diagnosis of children with egg allergy were 0.905, 0.872, 0.601, respectively. The sensitivity, accuracy and specificity of allergen IgE in the diagnosis of children with egg allergy were 0.929, 0.894, 0.600, respectively. The sensitivity, accuracy and specificity of combined detection of egg allergy were 0.976, 0.957, 0.831, respectively. Conclusion The combined detection of egg component protein, galactose agglutinin-3 and allergen IgE antibody is of high sensitivity, accuracy and specificity in the diagnosis of egg allergy, has high value in clinical application.
2018 Vol. 15 (32): 88-91 [Abstract] ( 210 ) HTML (1 KB)  PDF (575 KB)  ( 93 )
92 Value of NSE, ProGRP, CYFRA21-1 and CEA for chemotherapy efficacy evaluation in patients with limited and extensive stage of small cell lung cancer
ZHOU Xusheng1,2 CUI Zhaolei1,2 LIN Yingying1,2 HU Minhua1,2 CHEN Yan1,2
Objective To investigate the value of neuron specific enolase (NSE), pro-gastrin-releasing peptide (ProGRP), soluble fragment of cytokeratin 19 (CYFRA21-1) and carcinoembryonic antigen (CEA) for chemotherapy efficacy evaluation in patients in limited stage (LS) or extensive stage (ES) of small cell lung cancer (SCLC). Methods A total of 247 SCLC patients, first visit and after 2 cycles of chemotherapy, were selected from January 2014 to December 2016 in Fujian Cancer Hospital, and 86 patients in LS group (first visit 44 cases, after 2 cycles of chemotherapy 42 cases) and 161 in ES group (first visit 98 cases, after 2 cycles of chemotherapy 63 cases). Expression levels of serum NSE, ProGRP, CYFRA21-1 and CEA of patients were detected via electrochemiluminescence assay before and after treatment. Results Levels of serum NSE, ProGRP, CYFRA21-1 in the two groups were significantly lower after treatment than those before treatment (P ＜ 0.05). Spearman correlation analysis manifested that, in LS group NSE level was positively correlated to ProGRP and CYFRA21-1 levels, and the expression of CYFRA21-1 was positively correlated to ProGRP and CEA levels (P ＜ 0.05). In ES group either two indicators were positively correlated to the other (P ＜ 0.01). NSE had the highest value of areas under the ROC curve in both groups (LS group: 0.924, ES group: 0.920), with secondly ProGRP (LS group: 0.823, ES group: 0.820), that were both higher than CYFRA21-1 (LS group: 0.748, ES group: 0.806) and CEA (LS group: 0.589, ES group: 0.660). Moreover, the sensitivity of NSE in assessing treatment efficacy in LS group AND ES group was 86.4% and 95.9%, respectively, and the specificity was 92.9% and 79.4%, respectively. Conclusion NSE, ProGRP, CYFRA21-1 and CEA could be biomarkers in evaluation of chemotherapy efficacy in patients with SCLC, and NSE and ProGRP retain relatively higher sensitivity and specificity.
2018 Vol. 15 (32): 92-96 [Abstract] ( 223 ) HTML (1 KB)  PDF (733 KB)  ( 81 )
97 Expression and significance of microRNA-221 in non-small cell lung cancer
HE Jiayong1 YANG Chenchen2 XU Qian2
Objective To investigate the expression and significance of microRNA-221 in non-small cell lung cancer (NSCLC). Methods A total of 53 NSCLC cases′ clinical material were collected from Cancer Hospital Affiliated of Xinjiang Medical University from January 2010 to January 2011, and all the cases accepted pulmonary lobectomy and systematic mediastinal lymphadenectomy. Levels of miRNA-221 in the NSCLC and adjacent tissues were examined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The relationship between expression level of miRNA-221 and clinic pathological parameters were analyzed. Kaplan-Meier was used to analyze the relationship between miRNA-221 expression and overall survival (OS). Results The relative expression of miRNA-221 in NSCLC tissues was (0.0234±0.0002), and it was (0.0095±0.0003) in adjacent tissues. The relative expression of miRNA-221 in NSCLC tissues was higher than that in adjacent tissues (P ＜ 0.05). There were 31 cases having relative higher expression. The level of miRNA-221 was significantly higher in TNM stage Ⅲ than that in TNM stage Ⅰ-Ⅱ(P ＜ 0.05). Kaplan-Meir survival analysis results manifested that the median OS of patients with miRNA-221 high expression was 36.4 months. There was no significant difference in OS between miRNA-221 low expression and high expression patients(P ＞ 0.05). Conclusion miRNA-221 is a potential molecular marker for the prognosis of lung cancer, which may be involved in the development and progression of NSCLC.
2018 Vol. 15 (32): 97-100 [Abstract] ( 235 ) HTML (1 KB)  PDF (528 KB)  ( 122 )
101 Expressions of claudin-3， claudin-7 in nasopharyngeal carcinoma
CHEN Ru1 SU Xiaoqing1 PAN Shenghua2 CHEN Liaozhi1 ZHOU Jing1 HUANG Yideng3▲
Objective To explore the expression of claudin-3, claudin-7 in patients with nasopharyngeal carcinoma(NPC) and to analyze the relationship between the proteins and clinical significance. Methods Fifty-two archival paraffin embedded tissue specimens of nasopharyngeal were retrospectively analyzed from January 2014 to January 2016 at the Third Affiliated Hospital of Wenzhou Medical University. Another 20 samples of Histologically normal nasopharyngeal tissues were also obtained as control group. Immunohistochemstry was used to detect the expression of claudin-3 and claudin-7 in the different tissues, and the relationship was analyzed between the expression and clinic pathological parameter. The relationship of the expression levels of claudin-3 and claudin-7 in nasopharyngeal carcinoma tissues was analyzed. Results There was no statistically significant difference in the positive expression rate of claudin-3 (51.92%) between nasopharyngeal carcinoma and adjacent non-neoplastic tissues (45.00%) (P ＞ 0.05). While the positive expression rate of claudin-7 in nasopharyngeal carcinomas (26.92%), in comparison with the adjacent non-neoplastic tissues (55.00%), there was significant difference (P = 0.025). The positive expression rate of claudin-3 in the Ⅲ+Ⅳ stage patients was significantly higher than that in the Ⅰ+Ⅱ stage patients (P = 0.002). Compared with the positive expression rate of claudin-7 in nasopharyngeal carcinomas without cervical lymph node metastasis (9/15), the positive expression rate in nasopharyngeal carcinomas with cervical lymph node metastasis was remarkably lower (5/37) (P = 0.001). Correlation analysis results showed that there was no relationship between expression of claudin-3 and claudin-7 (r = 0.2696, P = 0.0532). Conclusion claudin-3 relates to the TNM tumor staging, in consideration of the effect in the nasopharyngeal carcinoma progress. claudin-7 decreased expression plays a role in the occurrence and development of nasopharyngeal carcinoma. claudin-3 and claudin-7 may be novel biomarkers for the new target for nasopharyngeal carcinoma treatment.
2018 Vol. 15 (32): 101-105 [Abstract] ( 189 ) HTML (1 KB)  PDF (458 KB)  ( 124 )
106 Effect of hyperbaric oxygen combined with vacuum sealing drainage for treating refractory bedsore
SHEN Chen1 GUO Dazhi2▲
Objective To investigate the efficacy of vacuum sealing drainage (VSD) combined with hyperbaric oxygen in treating refractory bedsore. Methods From January 2016 to December 2017, in Beijing Fengsheng Special Hospital of Traditional Medical Traumatology and Orthopaedics and Navy General Hospital, the clinical data of 37 patients with Ⅲ and Ⅳ degree bedsores was retrospectively analyzed, 20 cases were treated with VSD only (regarded as VSD group), 17 cases were treated with VSD combined with hyperbaric oxygen (regarded as VSD combined with HBO group), patients of both groups had the same basic nursing plan. At 2 weeks, 3 weeks, 1 month after treatment, specimens of wound from two groups were taken respectively, the CD34 immunohistochemistry was performed by streptavidine-biotin complex (SABC), the number of wild vascular cross was observed as capillary density value under high magnification microscope to assess the hyperplasia of granulation tissues of wound. The capillary density value and the visual wound observation were indicators for evaluation of clinical efficacy. Results The capillary density of VSD combined with HBO group was higher than that of VSD group at each time point, the differences were statistically significant (P < 0.05). Compared with the VSD group, the VSD combined with HBO group had higher effective rate and less average time, the differences were statistically significant (P < 0.05). Conclusion VSD combined with hyperbaric oxygen can effectively promote the rapid growth of new granulation tissue. It has good curative effect and short course of treatment, and provides a new strategy for clinical treatment of refractory bedsore.
2018 Vol. 15 (32): 106-108,117 [Abstract] ( 244 ) HTML (1 KB)  PDF (687 KB)  ( 123 )
109 Effects of psycho-educational multifamily group therapy on family burden of patients with schizophrenia
QIAO Yunshuan ZHANG Shiqiao▲ CUI Weidong LI Jing
Objective To explore the effect of psycho-educational multifamily group (PMFG) therapy on family burden of patients with schizophrenia. Methods In June 2015, 200 patients with severe mental disorder in rural areas managed by hospitals of towns and villages in Hengshui City of Hebei Province were randomly selected, and 73 patients with schizophrenia were confirmed after re-examination diagnosis and exclusion. They were divided into research group and control group by random number table method, with 36 cases in the research group and 37 cases in the control group. All patients in two groups were systematically terated with antipsychotic drugs, the research group was given the model of PMFG, the control group was given conventional pattern of community management administrative intervention. The family burden of the two groups was assessed by using symptom checklist 90 (SCL-90), family burden interview schedule (FBIS) and social support revalued scale (SSRS). Results The negative rates of family financial burden, home entertainment and physical health of family members in the FBIS of the research group were significantly higher than those of the control group, the differences were statistically significant (P < 0.05 or P < 0.01). After intervention, all the scores of SCL-90 of the research group were lower than the national norm, and the scores of depression and hostility factors of the research group were lower than the national norm (P < 0.05), the scores of compulsion, interpersonal relationship, anxiety and psychiatric factors of the research group were significantly lower than the national norm (P < 0.01). After intervention, among all factors of SCL-90, the scores of compulsion, hostility and psychiatric factors of the research group were significantly lower than those of the control group (P < 0.05). The scores of somatization, interpersonal relationship, depression, anxiety, terror and paranoia of the research group were significantly lower than those of the control group (P < 0.01). The total score of the SSRS of the research group was higher than that of the control group, the difference was statistically significant (P < 0.05). Conclusion PMFG can reduce the residual symptoms of patients with mental illness, at the same time, it can improve the family function of the families of mental illness patients, reduce the psychological pressure of the families of the patients and improve their quality of life.
2018 Vol. 15 (32): 109-113 [Abstract] ( 193 ) HTML (1 KB)  PDF (642 KB)  ( 151 )
114 Effect of electroacupuncture on metabolic state of patients with metabolic syndrome caused by atypical antipsychotics agents
ZHANG Peng ZHU Xianghua HE Qing
Objective To explore the effect of electroacupuncture on metabolic state of patients with metabolic syndrome caused by atypical antipsychotics agents. Methods Ninety-two patients with metabolic syndrome caused by atypical antipsychotics agents in Xuzhou Oriental People′s Hospital in Jiangsu Province from January 2016 to December 2017 were selected as research subjects. They were randomly divided into study group (n = 46) and control group (n = 46) according to envelope method. On the basis of atypical antipsychotic agents, both groups were treated with diet control and exercise intervention. In addition, the study group was treated with electroacupuncture, 30 minutes each time, once a day, every 5 d treatment was followed by 2 d interval, 1 month was as a course, total for 3 courses. The changes of blood glucose, blood lipids, blood pressure, liver and kidney function, body mass index and waist circumference before and after treatment were detected and compared between the two groups. During treatment, the side effect of the two groups was compared by the treatment emergent symptom scale (TESS). Results Compared with before treatment, blood glucose, blood lipids, blood pressure, body mass index, waist circumference and other indicators were improved after treatment in the study group (P < 0.05). Compared with the control group, blood glucose, blood lipid, blood pressure, body mass index, waist circumference and other indicators were improved in the study group after treatment, the differences were statistically significant (P ＜ 0.05). There was no significant difference in liver and kidney function between the two groups before and after treatment (P ＞ 0.05). There was no significant difference in TESS score between the two groups (P ＞ 0.05). Conclusion On the basis of diet control and exercise intervention, electroacupuncture can effectively improve the metabolic state of patients with metabolic syndrome caused by atypical antipsychotics agents. It has good safety and is worthy of clinical trial.
2018 Vol. 15 (32): 114-117 [Abstract] ( 234 ) HTML (1 KB)  PDF (588 KB)  ( 74 )
118 Clinical effect of the comprehensive treatment for postoperative intracranial infection based on the characteristics of pathogenic microorganisms
YUAN Huaitao1,2 HUANG Liangwen2 REN Jianwei2 LIU Jianmin2
Objective To summarize the experience of clinical diagnosis and treatment about the intracranial infection after craniocerebral operation, and to explore an effective method for the treatment of postoperative intracranial infection. Methods By retrospective analysis, 26 patients with intracranial infections after operation admitted to the First Affiliated Hospital of Guangzhou University of Chinese Medicine from January 2007 to March 2015 were selected. All of the cases were treated with comprehensive treatment, such as cerebrospinal fluid cytology examination, bacterial culture, intravenous and intrathecal use of Ceftriaxone Sodium or other sensitive antibiotics. The conditions of cerebrospinal fluid bacterial culture, choice of antibacterial agents, choice of cerebrospinal fluid drainage and intrathecal administration, healing cases and healing time were observed. Results The results of cerebrospinal fluid bacterial culture in 14 cases were positive (53.8%), among which, there were 4 cases of Staphylococcus aureus, 4 cases of Staphylococcus epidermidis, 2 cases of Enterobacter aeruginosa, 2 cases of Escherichia coli and 2 cases of Pseudomonas aeruginosa. All of 12 cases of patients with negative cerebrospinal fluid bacterial culture results of Staphylococcus aureus and Epidermis staphylococcus, Enterobacter aeruginosa, Escherichia coli and cerebrospinal fluid culture were taken intravenous Ceftriaxone Sodium and intrathecal drug delivery. The effect of Ceftriaxone Sodium on patients whose cerebrospinal fluid bacterial culture result was Pseudomonas aeruginosa was poor. Therefore, 1 case was converted to Cefoperazone Sodium and Tazobactam, and 1 case was converted to Meropenem. Fifteen patients selected continued lumbar cistern drainagee, 11 cases selected lumbar puncture, cerebrospinal fluid replacement. Twenty-six patients were all cured, the difference of the healing time between lumbar puncture patients and lumbar cistern drainagee patients was not statistically significant (P ＞ 0.05). Conclusion Based on the characteristics of pathogenic microorganisms, comprehensive therapy has a good effect on postoperative intracranial infection.
2018 Vol. 15 (32): 118-120,124 [Abstract] ( 234 ) HTML (1 KB)  PDF (599 KB)  ( 105 )
121 Clinical effect of Danqing Capsules combined with Ursodeoxycholic Acid Capules in the treatment of stone recurrence after cholecystolithotomy
ZHANG Zhiyou LIU Wensheng LI Ying ZHANG Shihai
Objective To investigate the clinical effect of Danqing Capsules combined with Ursodeoxycholic Acid Capules in the treatment of stone recurrence after gallbladder preserving lithotomy. Methods Sixty-nine patients who underwent endoscopic minimally invasive gallbladder preserving lithotomy in Nanjing Integrated Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine from April 2014 to April 2016 were selected as the research subjects. The patients were divided into control group (n = 34) and study group (n = 35) according to the random number table method. The patients in the control group were treated with Ursodeoxycholic Acid Capules, and the study group was treated with Ursodeoxycholic Acid Capules and Danqing Capsules. The patients′ clinical efficacy in the two groups after 2 months of treatment was compared, the patients′ gallbladder index in the two groups before and after treatment was compared, the patients′ adverse reactions in the two groups during the treatment were observed. After two years of follow-up, the recurrence was observed. Results The total effective rate of patients in the study group was higher than that of control group, there was a significant difference between the two groups (P ＜ 0.05). After treatment the thickness of the gallbladder wall in the two groups was lower than that before treatment, and the study group was lower than the control group (P ＜ 0.05), the gallbladder contraction rate was higher than that before treatment, and the study group was higher than the control group (P ＜ 0.05). The recurrence rate after treatment for 2 years of study group was lower than that of control group, the difference was statistically significant (P ＜ 0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P ＞ 0.05). Conclusion Danqing Capsules combined with Ursodeoxycholic Acid Capules in the treatment of gallstone recurrence after cholecystolithiasis is effective and safe, it can effectively reduce the recurrence of gallstones and improve the function of gallbladder, and it has certain clinical value.
2018 Vol. 15 (32): 121-124 [Abstract] ( 189 ) HTML (1 KB)  PDF (627 KB)  ( 117 )
125 Clinical value of B-type natriuretic peptide combined with Tei index for evaluating the cardiac function of patients with alcoholic cirrhosis
WANG Likun1 ZHANG Sumei2 WU Xueliang3 YANG Jielin4 YANG Ruimin1 HAN Chao1 WANG Yicheng1
Objective To investigate the important value of B-type natriuretic peptide (BNP) combined with Tei index for evaluating the cardiac function of patients with alcoholic cirrhosis. Methods A total of 73 patients with alcoholic cirrhosis treated in Department of Gastroenterology, the First Affiliated Hospital of Hebei North University from October 2015 to October 2016 were selected and they were divided into compensatory stage group (Child A group with 30 cases) and noncompensatory stage group (Child B group with 23 cases, Child C group with 20 cases) according to Child-Pugh liver function grading standard. Another 25 healthy volunteers were selected as control group. The plasma BNP level were detected by ELISA and were detected by colour ultrasound doppler. Statistics were used in analyzing the Tei index and it was compared among the groups. Results The plasma BNP level in Child B and Child C group were higher than those in Child A and control group, and the plasma BNP level in Child C group was higher than that in the control group, and there were significant differences (P ＜ 0.05). The Tei index of left ventricle and IRT/ET value in Child B and Child C group were higher than those in Child A and control group, and the Tei index of left ventricle and IRT/ET value in Child C group was higher than that in the control group, and all the differences were remarkably significant (P ＜ 0.01). While there was no difference in Tei index of right ventricle and IRT/ET value among all the groups (P ＞ 0.05). There was significantly positive correlation between plasma BNP and left ventricular Tei index in patients with alcoholic liver cirrhosis (r = 0.618, P ＜ 0.01). Conclusion Plasma BNP combined with Tei index could accurately evaluate the changes of cardiac function in patients with alcoholic cirrhosis and provide a scientific reference for clinical treatment.
2018 Vol. 15 (32): 125-128,134 [Abstract] ( 183 ) HTML (1 KB)  PDF (1017 KB)  ( 102 )
129 The development and practice of the precise usage and dosage rules of drugs in the preapproval system of outpatient prescription in our hospital
CHEN Lumei LIAO Lina LI Xin TAO Xia CHEN Wansheng WU Rong▲
Objective To develop and set precise usage and dosage rules for prescription pretrial system. Methods The precise usage and dosage were set based on the description of the medicine, the latest domestic and international instruction of prescription, and the consensus of experts, in order to complete the rules from multiple aspects. The all prescription date were selected from Changzheng Hospital, the Second Military Medical University from December 2016 to August 2017, and the average ratio of disqualified, unsuitable usage and overdosed defective prescription was analyzed. Results The average ratio of defective prescription was 19.17%, which was lower than the ratio (27.83%) before the setting the system （P = 0.019）. The ratio of unsuitable usage and overdosed defective prescription decreased （P ＜ 0.05）. Conclusion When a doctor prescribes a prescription, the pre-prescription review system runs refined rules to intercept unreasonable prescription in time, so that the ratio of unqualified prescription in outpatient decreases significantly.
2018 Vol. 15 (32): 129-134 [Abstract] ( 207 ) HTML (1 KB)  PDF (1163 KB)  ( 137 )
135 Influence of construction of care ward on psychological characteristics, sleep disorders and pain beliefs of chemotherapy patients with giant cell tumor of bone
HAN Lili1 CHEN Jiangtao2 KANG Wei1 YAO Chuntao1 HUANG Zhijiao1
Objective To discuss the influence of the construction of care ward on the psychological characteristics, sleep disorders and pain beliefs of chemotherapy patients with giant cell tumor of bone. Methods From May 2015 to May 2017, in the First Affiliated Hospital of Xinjiang Medical University, 88 chemotherapy patients with giant cell tumor of bone and were selected, they were divided into control group and observation group by random number table method, with 44 cases in each group. The control group was given routine nursing care, the observation group was given the using of care ward on the basis of control group. The psychological characteristics, sleep disorders and pain beliefs of patients in two groups were compared. Results Compared with before intervention, the proportion of patients of pessimistic despair, fear, anger, fantasy, selfishness, anxiety, loneliness, and denial in the two groups after intervention decreased, which of the observation group were lower than those of the control group, the differences were statistically significant (P < 0.05). Compared with before intervention, the scores of sleep time, sleep disorder, fall-sleep time, sleep efficiency, daytime function, sleeping drug scores in the two groups after intervention decreased, which of the observation group were lower than those of the control group, the differences were statistically significant (P < 0.05). Compared with before intervention, the scores of the pain control belief, the mood faith of pain, the drug conviction of pain, the pain disability belief and the belief of pain tolerance in the two groups after intervention increased, which of the observation group were higher than those of the control group, the differences were statistically significant (P < 0.05). Conclusion The construction of care ward applied to the intervention of patients with giant cell tumor of bone can effectively alleviate adverse psychological characteristics of patients, improve the condition of sleep disorders, promote the patients′ pain beliefs.
2018 Vol. 15 (32): 135-138 [Abstract] ( 228 ) HTML (1 KB)  PDF (536 KB)  ( 102 )
139 Effects of comprehensive evaluation and individualized intervention on wound healing and quality of life in diabetic patients
CHEN Zhuo CHEN Changxiang LIANG Jing
Objective To explore the effect of comprehensive evaluation and individualized intervention on chronic wounds and quality of life of patients with diabetes mellitus. Methods From September 2016 to August 2017, in Hebei Province Kailuan General Hospital and Tangshan Gongren Hospital, 88 patients of diabetes mellitus with chronic wounds were selected and divided into intervention group and control group according to the random number table method, with 44 cases in each group. Based on the wet healing treatment, the intervention group was given comprehensive assessment and individualized intervention, the control group was given the routine nursing measures. Pressure ulcer scale for healing (PUSH) was used to evaluate the wound healing status of the two groups and compare the wound healing days at each stage.The health survey scale (SF-36) was used for comparing the quality of life in the two groups after intervention. Results After intervention for 7, 14, 21 days, the PUSH score of the two groups were significantly lower than those before intervention, the differences were statistically significant (P < 0.05). The comparison of PUSH score between the two groups on the 7th day after intervention showed no statistically significant difference (P < 0.05). After intervention for 14, 21 days, the PUSH score of the intervention group were lower than those of the control group, the differences were statistically significant (P < 0.05). The duration of wound healing at 25%, 50%, 75% and 100% of the intervention group were significantly shorter than those of the control group, the differences were statistically significant (P < 0.05). After intervention, the SF-36 scores in each dimension of the intervention group were higher than those before intervention, the differences were statistically significant (P < 0.05), while the control group showed improvement in physiological function, physiological function and body pain comparing with those before intervention, the differences were statistically significant (P < 0.05). After intervention, the physiological function, physical pain, energy, social function, mental health and overall health scores of the intervention group were higher than those of the control group, the differences were statistically significant (P < 0.05). Conclusion Through comprehensive evaluation, the individualized intervention on the diabetes mellitus patients with chronic wounds can accelerate the wound healing speed and improve the quality of life.
2018 Vol. 15 (32): 139-142,146 [Abstract] ( 198 ) HTML (1 KB)  PDF (603 KB)  ( 109 )
143 Effects of nursing information system construction on quality nursing service in level-3 general hospitals
XIE Yuqin1 WEN Xiaoping1 WEN Xianxiu2 LIAO Changju1
Objective To explore the effects of the construction of nursing information system (NIS) on quality nursing service in level-3 general hospitals. Methods From September 2016 to September 2017, in Zigong First People′s Hospital, 260 hospitalized patients were selected and divided into research group and control group by random number table method, with 130 cases in each group. Twenty-eight nurses were selected at the same period, they were assigned to research group and control group randomly, with 14 nurses in each group. The research group was given quality nursing service based on NIS, the control group was given quality nursing service only. The quality of nursing, patients' satisfaction and nurses′ satisfaction after nursing in two groups were compared. Results The health education scores, specialist nursing scores, disease severity assessment scores and basic nursing scores of nursing quality in research group were higher than those in control group, the differences were statistically significant (P < 0.05); the satisfaction scores of humanistic care, environmental facilities, professional skills and sense of responsibility of nurses in research group were higher than those in control group, the differences were statistically significant (P < 0.05); the scores of work load, nursing management, relationship with colleagues, recognition from work of nurses in research group were higher than those in control group, the differences were statistically significant (P < 0.05). Conclusion The construction of NIS can help improve the quality nursing service in level-3 general hospitals and improve the satisfaction of both the patients and nurses.
2018 Vol. 15 (32): 143-146 [Abstract] ( 218 ) HTML (1 KB)  PDF (542 KB)  ( 100 )
147 Efficacy observation of Sipo Pigmentum treatment for skin allergy after PICC catheterization in cancer patients
TIAN Shuiqing1 MA Hongmei2 WAN Yonghui1 YANG Liting1
Objective To investigate the efficacy of Sipo Pigmentum treatment for skin allergy after peripherally inserted central catheter (PICC) catheterization in cancer patients. Methods From April 2016 to May 2017, 60 cancer patients with skin allergy after PICC catheterization treated in Department of Oncology, Renmin Hospital of Wuhan University were selected, they were divided into experiment group and control group by random number table method, with 30 cases in each group. The experiment group was given Sipo Pigmentum, and the control group was given Eloson Cream. The improvement of allergic skin and the incidence of related complications after medication were observed and recorded. Results After medication for 5, 10 days, the curative effect of the experiment group was better than that of the control group, the differences were statistically significant (P < 0.05 or P < 0.01); the skin allergy cure time of the experiment group was significantly shorter than that of the control group, the difference was highly statistically significant (P < 0.01). The incidence of detubation, infection of the experiment group were lower than those of the control group, the differences were statistically significant (P < 0.05). Conclusion Sipo Pigmentum has an outstanding efficacy for skin allergy after PICC catheterization, which decreases the incidence of infection and other ralated complications.
2018 Vol. 15 (32): 147-150 [Abstract] ( 172 ) HTML (1 KB)  PDF (575 KB)  ( 81 )
151 Two cases of atypical amniotic fluid embolism
LI Min YANG Meilin▲
Atypical amniotic fluid embolism is a complication of delivery, which is easily misdiagnosed, unpredictable and with high maternal mortality. This article reviewed 2 cases of atypical amniotic fluid embolism, and to discusses the clinical manifestation, diagnosis and treatment of atypical amniotic fluid embolism, and summarized the key points of successful rescue. In this study, typical amniotic fluid embolism symptoms was absent in 2 patients, the main manifestation was sharp deterioration of coagulation function, both patients were successfully rescued and retained the uterus. Atypical amniotic fluid embolism lacks circulation and respiratory symptoms, and early diagnosis is difficult, which is easily confused with postpartum hemorrhage. Correcting coagulation function as soon as possible and maintaining effective contractions are the key to successful rescue and the prerequisite for successful preservation of the uterus.
2018 Vol. 15 (32): 151-154 [Abstract] ( 210 ) HTML (1 KB)  PDF (387 KB)  ( 122 )
155 Study on current situation and social work intervention of "three noes" patients in primary and outpatient emergency treatment
LIAO Shengwu1 TAN Bihui2 CHEN Bao′an3 WANG Feng′e4
In "three noes" patients emergency medical treatment, medical institutions can assume the moral responsibility, practice, implementation of the effective treatment. The main problems are medical arrears problems, and lack of professional treatment of the system of embarrassment. The basic strategy to solve these problems include accepting social work in perfect "three noes persons" treatment of social organization, to strengthen the "three noes persons" treatment of publicity, the establishment of system of "three noes persons" treatment, improve the quality and level of treatment work of "three noes persons".
2018 Vol. 15 (32): 155-159 [Abstract] ( 191 ) HTML (1 KB)  PDF (620 KB)  ( 77 )
160 Analysis of medical saffs on MBTI personality type test
XU Xiaohuan ZENG Weilong OU Ping WANG Hongmei
Objective To study the personality characteristics of four types of medical staffs, medicine staffs, nursing staffs and technical staffs. Methods From October 2016 to July 2017, 682 medical staffs from Guangxi Zhuang Autonomous Region Liuzhou Worker′s Hospital and Liuzhou Maternity and Child Healthcare Hospital were tested by Myers briggs type indicator (MBTI) personality scale, the results were collected and analyzed by SPSS 18.0 softruare. Results There was no significant difference in the dimensions of "extraverion E - introversion I" and "sensation S - intuition N" (P ＞ 0.05). There was significant difference of rational function dimension of "thinking T - emotion F" and life attitude orientation dimension of "judgment J - perception P" (P ＜ 0.05). This research found that most of them were ESTJ (101 medical staffs, accounting for 37.7%; 80 nursing staffs, accounting for 25.4%; 33 medical technicians staffs, accounting for 42.3%; 5 pharmaceutical personnel, accounting for 23.8%; a total of 219 people, accounting for 32.1%), followed by ISTJ (12.2% in total) and ENTJ (11.3% in total). Conclusion The distribution of medical staff's personality type based on MBTI scale analysis is mostly ESTJ, and the dimension of "judgment J - perception P" is the main personality characteristic of medical staffs. The test results can be applied to the team building, career development planning, training, motivation, recruitment and selection of medical personnel.
2018 Vol. 15 (32): 160-163 [Abstract] ( 272 ) HTML (1 KB)  PDF (662 KB)  ( 270 )
164 Application of evidence-based pharmacy in hospital clinical pharmacy practice
WAN Jun CUI Jie
This paper introduces the basic concepts, evidence classification and implementation methods of evidence-based pharmacy, and elaborates its application value in clinical pharmacy, including clinical drug intervention, adverse drug reaction monitoring, pharmacy counseling service and pharmacoeconomics. The evidence-based pharmacy thought can break through the limitations of traditional pharmacy methods, optimize the pharmacy practice, solve problems of pharmacy more objectively and scientifically, which is the inevitable trend of the future development of pharmacy. The purpose of this paper is to provide a reference and guidance for the clinical pharmacists to better understand and master the basic ideas and methods of evidence-based pharmacy.
2018 Vol. 15 (32): 164-167 [Abstract] ( 268 ) HTML (1 KB)  PDF (612 KB)  ( 95 )
168 Discussion on teaching reform of manufacturing production quality management course in higher vocational colleges
LIU Yongli SHI Lei XU Jun
The course "good manufacturing practices of drug" is one of the important public subjects of the major “drug manufacturing technology” in vocational college. This paper analyzes the problems existing in the teaching mode of traditional pharmaceutical production quality management course according to the training objective of pharmaceutical production technology specialty in higher vocational colleges, and mainly discusses the teaching reform of the course. Through the introduction of a variety of teaching modes, including experiential teaching, combination of work and study, task-oriented teaching, learning promotion through competition and the establishment of network platform courses, the teaching effect of drug production quality management courses was improved, so that the good manufacfacturing practices (GMP) concept was truly in the minds of students, creating a good start for students′ employment and enterprises′ employment.
2018 Vol. 15 (32): 168-171,180 [Abstract] ( 251 ) HTML (1 KB)  PDF (738 KB)  ( 174 )
172 Application of flipped classroom under the guidance of working process-orientation teaching mode in "pharmaceutical service" training
LIU Yan YAN Xin LI Suying AN Shuo ZHANG Lei
To further promote the innovation of pharmacy teaching reform in He′nan Medical College, improve the teaching level of practical training of "pharmaceutical service", enhance students′ "pharmaceutical service" professional skills, enhance students′ interest in learning, give play to students′ learning enthusiasm and innovation, we combined working process-orientation teaching mode with flipped classroom applying to the practical teaching of "pharmaceutical service" training in higher vocational college. Two classes with similar academic performance and learning ability were used as experimental subjects, and the two training contents were carried out in group experiment. The results is that, it is better than traditional teaching mode. This teaching method can increase the interaction between teachers and students, and also shorten the distance between teaching and work. It can highlight the dominant position of students, strengthen the training of students′ independent learning management ability, enhance students′ comprehensive employ ability, improve the teaching effect. It is a teaching method worthy of promotion.
2018 Vol. 15 (32): 172-175 [Abstract] ( 195 ) HTML (1 KB)  PDF (609 KB)  ( 119 )
176 Suggestions on improvement of human resources development and management in public hospitals under the background of new medical reform
HE Jingshu LI Nan HUANG Wei
With the continuous reform of Chinese medical and health service system, it has entered a deep-water zone that is difficult to overcome. Hospitals, as the main force in the medical reform process, face new opportunities and challenges, and the importance of hospital human resources management is even more prominent. In order to improve the status quo of human resources management in public hospitals, through the discussion of the problems existing in the process of human resources management in public hospitals in the process of new medical reform, the proposal to build a human-oriented human resource management system will help to improve the level of hospital medical services. Efficiency, realize modernization of public hospitals.
2018 Vol. 15 (32): 176-180 [Abstract] ( 195 ) HTML (1 KB)  PDF (617 KB)  ( 115 )
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