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2019 Vol. 16, No. 23 Published: 2019-08-15

	7	Bioinformatics analysis of the relationship between miRNA-494 and myocardial fibrosis in chronic heart failure
		LIANG Shan1 HE Yazhou1 LI Lijuan1 HUANG Caiyi1 WANG Qinggao2
		Objective To explore the mechanism of myocardial fibrosis in chronic heart failure patients by using bioinformatics analysis. Methods The GSE104150 dataset through GEO database was filtered out, the differentially expressed miRNAs between heart failure and healthy control was analyzed. The target genes of miR-494 were predicted, which should be performed the gene ontology (GO) enrichment analysis and signal pathway enrichment analysis. Results Compared with the healthy control, miRNA-494 was differentially expressed in the chronic heart failure patients. The GO analysis showed the target genes of miR-494 were enriched in cell components such as cytoplasm and nucleus, molecular functions as protein binding and biological functions as cell proliferation and migration, phosphatidylinositol-mediated signaling. The KEGG biological pathway was mainly enriched in TGFβ, PI3K/Akt, and MAPK signaling pathways. Conclusion It is speculated that miRNA-494 is differentially expressed in patients with chronic heart failure, and it has a certain correlation with the occurrence and development of myocardial fibrosis, further research is needed to confirm.
		2019 Vol. 16 (23): 7-11 [Abstract] ( 212 ) HTML (1 KB) PDF (1072 KB) ( 54 )

	12	Discussion on cellular and molecular mechanism of Fuzheng Huayu Recipe against myocardial fibrosis from the point of view of matrix metabolism
		ZHU Ke1 LOU Lixia1 QI Yifei1,2 GAO Yonghong1 LI Chunhong1 GUO Qianqian1 WU Dandan1 ZHANG Dongmei1
		Objective To observe the effect of Fuzheng Huayu Recipe (FZHY) on the extracellular matrix (ECM) metabolism of rat cardiac fibroblasts (CFs) induced by angiotensin Ⅱ (Ang Ⅱ), and to explore the mechanism of FZHY in treating myocardial fibrosis. Methods Differential adherent method was used to extract primary CFs from neonatal rats and subculture them. Cells can be divided into three groups, each group of 6 samples, add 2% FBS-DMEM (blank control group), 1 × 10-7 mol/L Ang Ⅱ (Ang Ⅱ group), 1 × 10-7 mol/L Ang Ⅱ + 100 μg/mL FZHY (FZHY group). Intervention after 48 h, collagentype Ⅰ(ColⅠ), ColⅢ, matrix metalloproteinases (MMP)-2, MMP-9 and TIMP-1 and TIMP-2 secretion were detected by the method of ELISA; ColⅠ, ColⅢ, MMP-2 and MMP-9 and TIMP 1 and TIMP-2 mRNA expression were detected by real-time fluorescent quantitative PCR method. Results Compared with blank control group, Col Ⅰ, Col Ⅲ content and mRNA expression of Ang Ⅱ group increased (P ＜ 0.01). Compared with Ang Ⅱ group, Col Ⅰ, Col Ⅲ content and its mRNA of FZHY group expression decreased (P ＜ 0.05 or P ＜ 0.01). Compared with blank control group, MMP-2, MMP-9 and TIMP 1 and TIMP-2 and its mRNA expression of Ang Ⅱ group increased (P ＜ 0.05). MMP-2/TIMP-2 and MMP-9/TIMP-1 showed a decreasing trend. Compared with Ang Ⅱ group, MMP-2, MMP-9 and TIMP 1 and TIMP-2 and its mRNA expression, and its MMP-2/TIMP-2 of FZHY group decreased (P ＜ 0.05 or P ＜ 0.01), and MMP-9/TIMP-1 had a tendency to decrease, but there was no statistical significance (P > 0.05). Conclusion FZHY can reduce the CFs ColⅠ, ColⅢ expression, its mechanism may be related to downgrade MMP-2, MMP-9 and TIMP 1 and TIMP-2 and its mRNA expression, reduce the MMP-2/TIMP-2, MMP-9/TIMP-1 ratio, regulating the metabolism of ECM.
		2019 Vol. 16 (23): 12-15 [Abstract] ( 174 ) HTML (1 KB) PDF (516 KB) ( 144 )

	16	Effect of Edaravone on acute lung injury in seawater drowning rats
		CHEN Zhuo1 SHAN Qi1 LI Hong2
		Objective To observe the therapeutic effect of Edaravone on acute lung injury in seawater drowning rats and explore its mechanism. Methods Forty healthy male SD rats were divided into control group (group C), Edaravone group (group E), seawater drowning group (group S) and treatment group (group SE) according to random number table method, with 10 rats in each group. Artificial seawater was injected into trachea to establish animal models: group C was injected with 2 mL/kg saline via tail vein after tracheal exposure; group E was injected with 2 mL/kg Edaravone injection via tail vein after tracheal exposure; group S was injected with 4 mL/kg artificial seawater after tracheal exposure + 2 mL/kg saline via tail vein; group SE was injected with 4 mL/kg artificial saline via trachea after tracheal exposure + 2 mL/kg Edaravone injection via tail vein. Six hours after the establishment of the model, rats were killed. The wet/dry weight ratio, protein concentration in bronchoalveolar lavage fluid, interleukin (IL)-6, IL-10, serum tumor necrosis factor-α (TNF-α) content and pathological changes of lung tissue were detected. The mRNA and protein expression of aquaporin (AQP)-1 and AQP5 were detected by RT-PCR and Western blot. Results The wet-dry ratio of lung tissue in group S was significantly higher than that in group C (P < 0.05), and that in group SE was lower than that in group S (P < 0.05). The protein content of bronchoalveolar lavage fluid in group S was significantly higher than that in group C (P < 0.05); the protein content of bronchoalveolar lavage fluid in group SE was lower than that in group S (P < 0.05). The serum levels of IL-6, IL-10 and TNF-α in group S and SE were significantly higher than those in group C (P < 0.05); the serum levels of IL-6 and TNF-α in group SE were significantly lower than those in group S, and the serum level of IL-10 was significantly higher than that in group S (P < 0.05). The expression of AQP1 and AQP5 in lung tissue of rats in group S was higher than that in group C (P < 0.05); the expression of AQP1 and AQP5 in lung tissue of rats in group SE was lower than that in group S (P < 0.05). Conclusion Edaravone can alleviate the acute lung injury caused by seawater drowning by regulating the expression of AQP1 and AQP5.
		2019 Vol. 16 (23): 16-20 [Abstract] ( 181 ) HTML (1 KB) PDF (744 KB) ( 69 )

	21	Protective effect of IL-1RA on airway inflammation and airway remodeling in COPD model rats
		LIU Jing WEI Fengqin ZHAO Weiye LUAN Nianxu LIU Xuedong▲
		Objective To investigate the protective effect of interleukin-1 receptor antagonist (IL-1RA) on airway inflammation and airway remodeling in rats with chronic obstructive pulmonary disease (COPD). Methods Thirty Wistar rats were induced by smoking and intratracheal injection of lipopolysaccharide (LPS). They were divided into control group, methylprednisolone treatment group and IL-1RA treatment group according to random number table method, with 10 rats in each group. Rats in each group were continuously intervened for 20 days starting on the 20th day. The control group was injected with 0.5 mg/(kg·d) saline, the methylprednisolone treatment group was injected with 0.5 mg/(kg·d) methylprednisolone, and the IL-1RA treatment group was subcutaneously injected with IL-lRA l00 pg per rat. The appearance of rats in each group was observed, blood gas was measured, lung histopathological examination was carried out, and mean alveolar number (MAN) and mean lining interval (MLI) were calculated. The content of High mobility group protein B1 (HMGB1) in bronchoalveolar lavage fluid (BALF) of rats in each group was detected by enzyme-linked immunosorbent assay, and the expression of HMGB1 in lung tissue of rats in each group was detected by Western blot. Results After treatment, there were significant differences in MAN and MLI among the three groups (P < 0.05). MAN in IL-1RA group was higher than that in methylprednisolone group and control group (P < 0.05), and MLI in IL-1RA group was significantly lower than that in methylprednisolone group and control group (P < 0.05). The expression of HMGB1 in BALF and lung tissue of three groups was significantly different (P < 0.05). The expression of HMGB1 in BALF of IL-1RA group was lower than that of methylprednisolone group and control group (P < 0.05), and the expression of HMGB1 in lung tissue was lower than that of methylprednisolone group and control group (P < 0.05). Conclusion IL-RA can reduce the expression of HMGB1 in lung tissue and alveoli of COPD rats, reduce airway inflammation, improve blood gas function and protect airway remodeling.
		2019 Vol. 16 (23): 21-25 [Abstract] ( 202 ) HTML (1 KB) PDF (767 KB) ( 117 )

	26	Research progress of circulating long chain non-coding RNA in colorectal cancer
		XU Wei1 LIU Yawen1 GONG Aihua2 XU Min1
		Long noncoding RNA (lncRNA) is dysregulated in many human cancers, including colorectal cancer (CRC), which is the third most common cancer in the world and is associated with poor prognosis; at the same time, some lncRNAs are involved in the development of CRC as tumor suppressor genes or oncogenes and are of great value in diagnosis, treatment and prognosis. LncRNA can enter the human circulatory system in the form of microbubbles, exosomes or protein complexes, forming stable circulating lncRNA. Its concentration level is different between healthy subjects and patients, which can be used as a novel, affordable, lightly accessible, non-invasive detection tool. This article briefly reviews recent advances in the biological properties of circulating lncRNA, focusing on the potential applications of circulating lncRNA as a biomarker for colorectal cancer screening and prognosis monitoring.
		2019 Vol. 16 (23): 26-29,37 [Abstract] ( 237 ) HTML (1 KB) PDF (628 KB) ( 125 )

	30	New progress in the study of differentiation of bone marrow mesenchymal stem cells into cardiomyocytes induced by Chinese medicine monomer
		DU Nannan1 WANG Yinfang2 ZHANG Peng2 LIU Zongjun1
		For a long time, cardiovascular diseases have threatened human life and health, and myocardial infarction is one of the most lethal diseases. Myocardial injury and apoptosis after myocardial infarction largely affect the quality of life of patients. Cardiomyocyte regeneration is the key to solve this problem. Based on the physiological characteristics of cardiomyocytes and bone marrow mesenchymal stem cells, this paper elaborates the signaling pathways and molecular mechanisms related to the directional differentiation of bone marrow mesenchymal stem cells into cardiomyocytes, and summarizes the regulatory mechanism and functional characteristics of traditional Chinese medicine in inducing differentiation, in order to provide reference for the prevention and treatment of cardiovascular diseases by traditional Chinese medicine.
		2019 Vol. 16 (23): 30-33 [Abstract] ( 257 ) HTML (1 KB) PDF (605 KB) ( 106 )

	34	Research progress of miRNA in the Toll-like receptor signaling pathway of rheumatoid arthritis
		ZHANG Na ZHANG Liyun ZHANG Gailian GAO Jinfang MA Dan LI Juan XU Ke
		Rheumatoid arthritis (RA) is an autoimmune disease with progressive destruction of cartilage and bone caused by chronic synovial hyperplasia. It has a high rate of disability and teratogenesis, and the etiology is not fully understood. At present, the treatment of RA mainly includes non-steroidal anti-inflammatory drugs, hormones, slow-acting anti-rheumatic drugs and biological agents. Although it improves the remission rate of the disease, it can not repair the damaged joints that have been formed, and there are many disadvantages, such as large side effects and high price. Therefore, it is urgent to find a new treatment method, and to understand the pathogenesis of RA in depth. Mechanisms are particularly critical. This review summarizes the regulatory role of miRNA in RA Toll-like receptor signaling pathway in order to provide reference for finding new therapeutic targets for RA.
		2019 Vol. 16 (23): 34-37 [Abstract] ( 190 ) HTML (1 KB) PDF (604 KB) ( 101 )

	38	Reserch progress of serum paraoxonase-1
		HE Lan1，2 TANG Wei3 YU Qin1，4
		Paraoxonase-1 (PON1) is a high density lipoprotein-related esterase. PON1 plays an important physiological role in human serum. Its expression and activity are mainly affected by PON1 gene single nucleotide polymorphism. The relationship between single nucleotide polymorphism and disease is not consistent with individuals or populations. The occurrence of disease is the result of combination of genetical, physical and chemical factors. The relationship and mechanism between PON1 and many diseases remain unclear. This article aims to explore the relationship between PON1 and coronary heart disease, diabetes mellitus, stroke and cancer, and the possible pathogenesis, and provide new ideas for exploring the treatment and prevention of related diseases.
		2019 Vol. 16 (23): 38-40,56 [Abstract] ( 276 ) HTML (1 KB) PDF (615 KB) ( 130 )

	41	Reserch progress of preoperative radiotherapy for locally advanced breast cancer
		YANG Siqi SONG Qibin GONG Hongyun
		Breast cancer is one of the most common malignant tumors in women. Despite the continuous improvement of current diagnosis and treatment technologies and the increasing proportion of early breast cancer in the new cases of breast cancer, local advanced breast cancer (LABC) is still a prominent clinical problem. LABC has become an important factor affecting the treatment effect of breast cancer due to its low overall survival rate, high recurrence rate and distant metastasis rate. Radiotherapy, including preoperative radiotherapy and postoperative radiotherapy, is currently recognized as an effective treatment that can control the metastasis of breast cancer and improve the survival rate of breast cancer patients. However, there are few clinical studies on preoperative radiotherapy. Preoperative radiotherapy is known to reduce tumor diameter, make tumor tissue and surrounding normal tissue fibrosis and prevent intraoperative tumor spread, which can improve the quality of life of LABC patients. In this paper, the research status of LABC and preoperative radiotherapy of LABC are reviewed.
		2019 Vol. 16 (23): 41-44 [Abstract] ( 213 ) HTML (1 KB) PDF (600 KB) ( 199 )

	45	Progress in evaluation of left ventricular systolic function by two-dimensional speckle tracking imaging
		WU Danni1 LI Ping2 TIAN Haiyan3
		Two-dimensional color Doppler echocardiography has been applied to evaluate cardiac function and structure for its advantages of fast, portable and economical operation. The measurement of ventricular systolic and diastolic function and ventricular volume has been completed by M-mode echocardiography and two-dimensional Simpson biplane method. However, for some special diseases or slight changes in left ventricular systolic function, the parameters of two-dimensional color Doppler echocardiography can not truly reflect the changes. With the gradual development of modern ultrasound medical imaging, two-dimensional speckle tracking imaging (2D-STI) makes up for many shortcomings of conventional two-dimensional color Doppler echocardiography. It can dynamically track the real-time echo speckle movement of myocardial tissue, and then evaluate left ventricular systolic function. This article reviews the research progress of 2D-STI on left ventricular systolic function.
		2019 Vol. 16 (23): 45-48 [Abstract] ( 174 ) HTML (1 KB) PDF (600 KB) ( 76 )

	49	Research progress on the mechanism of T and B lymphocytes involved in autoimmune thyroid disease
		LI Yang ZUO Xinhe HUA Chuan ZHAO Yong
		The incidence rate of autoimmune thyroid diseases (AITD) is on the rise. The interaction of complex environmental factors, genetic factors and endocrine hormone may be the causes of the disease. The pathogenesis of AITD is mainly the activation of T lymphocytes and B lymphocytes, which produce autoantibodies targeted at thyroid tissues, so as to cause different degrees of thyroid injury and produce functional changes. In T lymphocytes, helper T lymphocytes are closely related to AITD. It has many subtypes, such as Th1, Th2, Th17, Treg, and so on. Th1/Th2 cell imbalance and Th17 cell activation are involved in the occurrence of AITD, while Treg cells can play an important role in immune suppression function; B lymphocyte activation is the core pathological process of the occurrence of Graves′ disease, while Breg cells can inhibit the autoimmune response and regulate the effect of T lymphocytes, which is a potential therapeutic target of AITD. This paper reviews the mechanism of T and B lymphocytes involved in AITD, which provides some ideas for finding effective therapeutic approaches.
		2019 Vol. 16 (23): 49-52 [Abstract] ( 224 ) HTML (1 KB) PDF (609 KB) ( 177 )

	53	Research progress on the related factors of embryo damage
		ZHOU Qing LIU Shuaimei ZHANG Ruijin SHI Hui LIN Ning WU Yulin XU Haoqin
		With the development of society, people′s eating habits, working, lifestyle and living environment have all changed, resulting in the decline of physical quality of people of childbearing age. Nowadays, embryo damage is a common disease in obstetrics and gynecology, and its incidence rate is increasing year by year. The causes of this disease include chromosome abnormality, environmental factors, sperm status, abnormal endocrine, microbial infection, abnormal uterus, maternal immune abnormality, maternal and fetal blood group incompatibility and other risk factors. Its pathogenesis is still unclear and has become a hot spot in obstetrics and gynecology at present. Embryo damage seriously affects women′s physical and mental health, so it is necessary to provide theoretical basis for early diagnosis and intervention.
		2019 Vol. 16 (23): 53-56 [Abstract] ( 177 ) HTML (1 KB) PDF (590 KB) ( 216 )

	57	Study on the correlation between depression and parenting style in medical college students
		PENG Xiao1 ZHANG Jinpeng2 KONG Junhui2
		Objective To understand the relationship between depression of medical students and parenting style and provide evidence for improving medical school students′ mental health. Methods From January to March 2017, a questionnaire survey was conducted on depression and parenting styles among medical students in Beijing. The relationship between Self-rating Depression Scale (SDS) score and Egna Minnen Betr?覿ffende Uppfostran for Children (EMBU) score was analyzed among 5798 medical students. Results After controlling the general demographic variables related to the dependent variable depression score, it was found that the father′s emotional warmth, understanding, punishment, severeness, excessive interference, rejection, denial, and excessive protection five dimensional coefficients were -0.285, 0.524, 0.470, 0.975, 0.767, respectively; mother′s emotional warmth, understanding, overprotection, excessive interference, rejection, denial, punishment, and severe four dimensional coefficients were -0.278, 0.283, 0.726, 0.646, respectively (all P < 0.01). Conclusion More attention is required to pay on parenting style when consulting a depressive medical student.
		2019 Vol. 16 (23): 57-60 [Abstract] ( 168 ) HTML (1 KB) PDF (428 KB) ( 148 )

	61	Correlation analysis of mental health of medical staff and physical exercise in Shanghai
		TIAN Weijuan
		Objective To explore the relationship between physical exercise and mental health of medical staff. Methods From June to December 2018, multicenter methods and questionnaire were used for the investigating of the physical and exercise situation of 150 medical workers in Putuo District People′s Hospital (50 cases), Tongji Ten Hospitals Group (50 cases) and the Community Health Service Center (50 cases), the medical staff mental health and physical exercise were analyzed. Results A total of 150 questionnaires were distributed and 146 effective questionnaires were recovered, the effective recovery rate was 97.33%. Among them, 20 medical workers were functional section members, 15 medical workers of deputy section grade, and 15 medical workers of department grade; 18 clinical physicians, 15 attending physicians and 15 chief physicians; 24 nursing teachers and 24 nurses. From section member, deputy section level to regular section level, the higher the position of medical staff in functional section was, the lower the number of people who participate in exercise and had the intention to exercise, and the higher the number of people who feel pressure (P < 0.05). Among the clinicians, the higher the professional title was, the more they participated in and had the intention to exercise, while the less they had the feeling of pressure (P < 0.05). Among clinical nurses, nurses and senior nurses were under great pressure, while the numbers of exercise participants and those with exercise intention were poor, with no statistically significant difference (P > 0.05). Correlation analysis showed that the sense of pressure of medical staff were negatively correlated with their willingness to exercise and their participation in exercise (r = -0.535, P < 0.01; r = -0.852, P < 0.01). Conclusion The mental health status of medical staff is closely related to their participation in physical exercise, and the mental health status of medical staff who actively participate in physical exercise is obviously better than that of medical staff who do not participate in physical exercise and occasionally participate in physical exercise.
		2019 Vol. 16 (23): 61-63,72 [Abstract] ( 174 ) HTML (1 KB) PDF (700 KB) ( 115 )

	64	Clinical study on different manifestations of myocardial ischemia-reperfusion injury
		ZHANG Ying WANG Yazhu ZHANG Haoran LIU Chao ZHANG Aiwen SUN WANG Lexian DING Zhenjiang
		Objective To explore the risk factors and prognosis of different manifestations of myocardial ischemia-reperfusion injury. Methods Ninety-one patients who underwent direct percutaneous coronary intervention in Affiliated Hospital of Chengde Medical College from January to November 2015 were divided into groups according to different clinical manifestations of ischemia-reperfusion injury. Twenty-one cases in bradyarrhythmia group and 70 cases in control group 1; 9 cases in tachyarrhythmia group and 82 cases in control group 2; 28 cases in reperfusion hypotension group and 63 cases in control group 3. Medical history, diagnosis, operation and treatment plan, follow-up and summary were recorded. Results The incidence of right coronary artery and collateral circulation of criminals in bradyarrhythmia group was higher than that in control group 1, the incidence of left anterior descending coronary artery of criminals in bradyarrhythmia group was lower than that in control group 1, the differences were statistical significance (P < 0.05). The symptoms-visiting time of tachyarrhythmia group was earlier than that of control group 2, and the incidence of right coronary artery was higher in criminals than that of control group 2, the differences were statistical significance(P < 0.05). The incidence of anterior descending branch of left coronary artery, ejection fraction, 24 h β receptor blocker and 24 h ACEI/ARB of criminals in reperfusion hypotension group were lower than those in control group 3, while the incidence of right coronary artery and total cholesterol level of criminals in reperfusion hypotension group were higher than those in control group 3, the differences were statistical significance (P < 0.05). The survival curves were drawn according to the follow-up results. The Kaplan-Meier survival curves of patients with different clinical manifestations were obtained by Log Rank test, there was no significant difference (P > 0.05). Conclusion The criminals′ right coronary artery is a risk factor for ischemia-reperfusion injury. The presence of collateral circulation is of great significance in predicting bradyarrhythmia, short symptom-visit time, rapid arrhythmia and high total cholesterol level.
		2019 Vol. 16 (23): 64-68 [Abstract] ( 182 ) HTML (1 KB) PDF (513 KB) ( 85 )

	69	Effect observation of Astragalus Injection combined with large amount of Gamma Globulin in the treatment of acute severe viral myocarditis in children
		NIU Feng1 YAO Huihui2 CAO Su3▲
		Objective To explore the effect of Astragalus Injection combined with large amount of Gamma Globulin in the treatment of acute severe viral myocarditis in children. Methods A total of 78 children with acute severe viral myocarditis in the 988th Hospital of PLA Joint Logistics Support Force from January 2016 to January 2019 were selected and divided into control group (n = 39) and observation group (n = 39) according to random number table method. The control group received routine treatment, while the observation group received Astragalus Injection combined with a large amount of Gamma Globulin. The clinical efficacy, inflammatory factors, myocardial enzymes levels and safety of two groups were compared before and after treatment. Results Total effective rate of observation group was significantly higher than that of control group (P < 0.05). After treatment, levels of IL-8, IL-17 and TNF-α in two groups were significantly lower than those before treatment, and those in observation group were significantly lower than the control group (P < 0.01). After treatment, CK-MB, LDH and CK levels in observation group were significantly lower than those before treatment, and those in observation group were significantly lower than the control group (P < 0.01). There were no obvious adverse drug reactions such as abnormal liver and kidney function in both groups. Conclusion Astragalus Injection combined with a large amount of Gamma Globulin in the treatment of acute severe viral myocarditis in children has a definite effect, which is worthy of popularization and application.
		2019 Vol. 16 (23): 69-72 [Abstract] ( 182 ) HTML (1 KB) PDF (429 KB) ( 94 )

	73	Effect of different doses of Methylprednisolone in children with autoimmune encephalitis
		CHEN Fang SUN Suzhen WANG Wei DU Yakun MA Guilin TANG Hongxia ZHANG Xiaoqing LIU Kang
		Objective To study the effect of different doses of Methylprednisolone in children with autoimmune encephalitis. Methods One hundred and eighty patients with autoimmune encephalitis admitted to Children′s Hospital of Hebei Province from February 2014 to February 2018 were selected as research objects and treated with Methylprednisolone, in addition, they were divided into low dose group [10 mg/(kg·d), 60 cases], medium dose group [20 mg/(kg·d), 60 cases] and high dose group [30 mg/(kg·d), 60 cases] by random number table method. The levels of against N-methyl-Daspartic acid receptor (NMDAR) antibody in serum and cerebrospinal fluid and the levels of immunoglobulin indices before and after treatment were compared among the three groups. The incidence of adverse reactions and mortality rate during treatment was recorded among the three groups. Results Six and twelve months after treatment, the levels of anti NMDAR antibodies in serum and cerebrospinal fluid of three groups were lower than those before treatment and 1 month, 3 months after treatment (P ＜ 0.05), which of the high, medium dose group were significantly lower than the low dose group (P ＜ 0.05), however, there was no significant difference between the high dose group and the medium dose group (P ＞ 0.05). Six months after treatment, the levels of immunoglobulin A, immunoglobulin G and immunoglobulin M in serum of the three groups were lower than those before treatment (P ＜ 0.05), which of the high dose group and medium dose group were significantly lower than the low dose group (P ＜ 0.05), however, there was no significant difference between the high dose group and the medium dose group (P ＞ 0.05). There were no significant differences in the incidence of cutaneous pruritus, impaired liver and kidney function, gastrointestinal bleeding and mortality rate among the three groups (P ＞ 0.05). How ever, the incidence of increased blood pressure and weight gain in the high dose group and medium dose group was higher than that in the low dose group (P ＜ 0.05). Conclusion The 20, 30 mg/(kg·d) doses of Methylprednisolone in the treatment of children with autoimmune encephalitis can ensure the efficacy of immunotherapy, and make the side effects of hormones within the tolerance range, which is worthy of clinical promotion.
		2019 Vol. 16 (23): 73-77,81 [Abstract] ( 235 ) HTML (1 KB) PDF (514 KB) ( 95 )

	77	Level changes and clinical significance of serum amyloid A, lipoprotein phospholipase A2 and homocysteine in patients with acute ischemic stroke
		ZHANG Tan LUO Yi ZHANG Qiang
		Objective To investigate the level changes, clinical significance and diagnostic efficacy of serum amyloid A (SAA), lipoprotein phospholipase A2 (Lp-PLA2) and homocysteine (Hcy) in patients with acute ischemic stroke. Methods One hundred and twenty-two patients with acute ischemic stroke admitted to Suzhou Hospital of Integrated Chinese and Western Medicine (“our hospital” for short) from May 2017 to May 2018 were selected as the experimental group, and their serum levels of SAA, Lp-PLA2 and Hcy were measured. The patients were divided into mild group (n = 44), moderate group (n = 25) and severe group (n = 53) according to the National Institutes of Health stroke scale (NIHSS), and the patients were divided into large area group (n = 59), medium area group (n = 25) and small area group (n = 38) according to the area of the infarct. Another 50 healthy people in the same period in our hospital were selected as the control group. The serum levels of SAA, Lp-PLA2 and Hcy were compared between the experimental group and the control group, and between different subgroups of the experimental group. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic values of serum SAA, Lp-PLA2, Hcy in patients with acute ischemic stroke. Results The serum levels of SAA, Lp-PLA2 and Hcy in the experimental group were significantly higher than those in the control group (P ＜ 0.01). In the experimental group, there were highly significant differences in serum levels of SAA, Lp-PLA2 and Hcy in groups with different degrees of severity and groups with different infarct areas (P ＜ 0.01), the serum levels of SAA, Lp-PLA2 and Hcy gradually increased with the aggravation of nerve function injury and the increase of infarction area. The diagnostic efficacy of combined detection of the three indicators for acute stroke was better than that of single detection. Conclusion The serum levels of SAA, Lp-PLA2 and Hcy in patients with acute ischemic stroke are significantly increased, which are increased with the increase of severity and infarct area. The combined detection of the three indicators is of great significance for the early diagnosis, screening and determination of the severity of cerebral infarction in patients with acute ischemic stroke.
		2019 Vol. 16 (23): 77-81 [Abstract] ( 228 ) HTML (1 KB) PDF (577 KB) ( 114 )

	82	Influencing factors of delayed cure after microvascular decompression for hemifacial spasm
		WANG Mo LIANG Xingbo LIU Jiawen LUO Guoxuan ZHANG Yang ZHANG Yong
		Objective To investigate the influencing factors of delayed cure of hemifacial spasm (HFS) after microvascular decompression (MVD). Methods A total of 598 patients with HFS who underwent MVD surgery at the Guangdong Second People′s Hospital from January 2013 to December 2016 were enrolled. Among them, 97 cases of delayed cure were recorded as observation group. And 501 cases who were cured immediately recorded as control group. The general data of the two groups were compared and the risk factors of delayed cure was analyzed by Logistic regression analysis. Results The proportion of course of disease ≥5 years, and the long-term use of Carbamazepine, botulinum toxin and composite vascular compression in the observation group were higher than those in the control group (P < 0.05). There were no significant difference in gender, age and other data between the two groups (P > 0.05). Logistic regression analysis showed that the course of disease and intraoperative vascular compression were the risk factors for delayed cure (OR = 1.364, 2.551, P < 0.05). Conclusion The main influencing factors of the delayed cure after MVD in HFS patients include the course of disease and the degree of intraoperative vascular compression.
		2019 Vol. 16 (23): 82-85 [Abstract] ( 677 ) HTML (1 KB) PDF (684 KB) ( 121 )

	86	Pregnancy outcome of patients with high-grade squamous intraepithelial lesion after conization
		MA Quanfu1 DAI Xuan1 ZOU Miao1 WANG Chaonan1 ZHAO Yun2 HU Junbo3 WU Xufeng1
		Objective To discuss the effect of cervical conization on pregnancy outcome of patients with high-grade squamous intraepithelial lesion (HSIL). Methods One hundred and fourteen patients with HSIL treated by cervical conization in Hubei Meternal and Child Health Hospital were included from December 2003 to December 2017, and all the patients were primigravid and successful delivery. Among them, 74 cases received high frequency wave loop resection (LEEP) and 40 cases received cold knife conization (CKC). The pregnancy outcome, delivery mode were compared between the two groups, and the effect of the interval time from pregnancy to operation on pregnancy outcome was observed. Results There were no significant differences in gestational weeks, preterm birth rate, premature rupture of membranes rate, low birth weight infants rate, average neonatal weight, cesarean section rate, and distribution of cesarean section indications between the two groups (P > 0.05). Among 114 delivery cases, the cesarean section rate was 70.2%. The interval time from pregnancy to operation had no significant effect on pregnancy outcome (P > 0.05). Conclusion Different cervical conization methods and the interval time from conization to pregnancy had no significant effect on the postoperative pregnancy outcome, but these women have tendency to choose cesarean section after conization.
		2019 Vol. 16 (23): 86-89 [Abstract] ( 211 ) HTML (1 KB) PDF (535 KB) ( 176 )

	90	Effect of two non-invasive positive pressure ventilation modes combined with Poractant Alfa in the treatment of neonatal respiratory distress syndrome
		HUANG Dan WANG Weijie GENG Shanshan HUA Jing▲
		Objective To approach effect of two non-invasive positive pressure ventilation modes combined with Poractant Alfa (Curosurf) in the treatment of neonatal respiratory distress syndrome. Methods Clinical data of 120 children with neonatal respiratory distress syndrome in Shanghai First Maternity and Infant Hospital, Tongji University Medicine School from December 2017 to December 2018 were analyzed, and they were divided into two groups by different ventilation modes, treatment group Ⅰ (NCPAP ventilation mode) 60 cases and treatment group Ⅱ (SiPAP ventilation mode) 60 cases. The PaO2, partial pressure of carbon dioxide in artery (PaCO2), forced expiratory volume in 1 s (FEV1), FEV1%Pred and FEV1/forced vital capacity (FVC) of children in two groups before and after treatment were observed; the non-invasive ventilation time, oxygen therapy time, clinical symptom remission time, hospitalization duration, clinical treatment total effect rate of children in two groups were observed. Results The PaO2, PaCO2, FEV1, FEV1%Pred, FEV1/FVC% of children in two groups before treatment were compared, the differences were no statistical significance (P > 0.05). After treatment, the PaO2, FEV1, FEV1%Pred, FEV1/FVC% of children in two groups were higher than before treatment, PaCO2 were lower than before treatment, and PaO2, FEV1, FEV1%Pred, FEV1/FVC% of children in treatment group Ⅱ were higher than treatment group Ⅰ, PaCO2 were lower than treatment group Ⅰ; the non-invasive ventilation time, oxygen therapy time, clinical symptom remission time, hospitalization duration of children in treatment group Ⅱ were shorter than treatment group Ⅰ, clinical treatment total effect rate of children in treatment group Ⅱ was higher than treatment group Ⅰ, the differences were statistical significance (P < 0.05). Conclusion SiPAP ventilation mode combined with Curosurf in the treatment of neonatal respiratory distress syndrome, can improve blood oxygen partial pressure, pulmonary function of children obviously, decrease treatment time, and increase clinical treatment efficiency, it is worthy of clinical application.
		2019 Vol. 16 (23): 90-93 [Abstract] ( 212 ) HTML (1 KB) PDF (534 KB) ( 40 )

	94	Effect of health education combined with sex hormone supplement in 130 patients with functional hypothalamic amenorrhea
		LI Jun1 MAO Lele2 BAI Wenpei2▲
		Objective To observe the effect of health education combined with sex hormone supplement in the treatment of patients with functional hypothalamic amenorrhea (FHA). Methods From January 2016 to August 2017, 130 cases with FHA in the Gynecological Outpatient Department of Beijing Shijitan Hospital and Beijing Aerospace General Hospital were selected, including 70 cases of clear history of weight loss, 36 cases of mental stimulation and 24 cases of hyperactivity. After the exclusion of sex hormone contraindications, patients were treated with Fenmoxone (2/10) for 28 days, one course of treatment, all were treated for 6 courses of treatment. Five sex hormones, such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone (T), pituitary prolactin (PRL), and progesterone (P) were measured before and after 6 months of administration. The recovery of menstrual cycle and the improvement of symptoms were observed during the 6-12 months followe-up. Results There were 99 patients with menstruation recovery in 130 patients after treatment, and the total effective rate was 76.2%. The levels of FSH, LH and E2 decreased before administration, and LH decreased more significantly. The levels of FSH, LH, E2 and P increased to different degrees at 6 months after administration (P < 0.01). The level change of T and PRL was not obvious (P > 0.05). Compared with before administration, the uterine volume increased and endometrium thickened after 6 courses of administration, and the differences were highly statistically significant (P < 0.01). Conclusion Sex hormone supplementation while the health education can significantly improve the hormone level, uterine volume, endometrium thickening and recover menstruation as soon as possible in patients with functional hypothalamic amenorrhea, and prevent a series of problems caused by osteoporosis and genital atrophy, improve the low estrogen caused symptoms such as menopause syndrome.
		2019 Vol. 16 (23): 94-97 [Abstract] ( 157 ) HTML (1 KB) PDF (505 KB) ( 152 )

	98	Discussion on the relevant factors influencing the prognosis of postoperative gastroparesis syndrome of digestive system malignant tumor
		TAN Yabin1* ZHOU Qin2* HU Ye3 YANG Ying1 FENG Baoyue1 ZUO Minghuan2
		Objective To explore the influencing factors of prognosis of postoperative gastroparesis syndrome (PGS) in patients with digestive system malignant tumor. Methods A clinical retrospective analysis was conducted on 91 patients with PGS of digestive system malignant tumor treated in four grade A tertiary hospitals, namely, Dongfang Hospital of Beijing University of Chinese Medicine, Cancer Hospital of Chinese Academy of Medical Sciences, Chinese PLA General Hospital and Peking University People′s Hospital from January 2013 to December 2015. 51 cases of patients with PGS of digestive system malignant tumor who achieved the favorable prognosis criteria within 7 days (7 day included) were selected as observation group; and 40 cases of patients failed in reaching the favorable prognosis of gastroparesis syndrome within 14 days (14 day included) were regarded as control group. 13 relevant factors that may influence the prognosis of PGS of digestive system malignant tumor were analyzed by the adoption of single factor chi-square test and t test. Results There were no statistically significant differences in terms of patients′ age, gender, body mass index, tumor metastasis, the placement of nasogastric tube, patterns of nutrition support, serum albumin amounts three days before admitting, hypertension, coronary heart disease and diabetes(P ＞ 0.05). The hemoglobin content 3 day before admitting in the observation group was higher than that of control group, the mean gastric tube drainage 3 day before admitting in the observation group was significantly lower than that of control group, the differences were statistically significant (P ＜ 0.05). Conclusion The improvement on hemoglobin levels and the control of the gastric tube drainage in patients with PGS of digestive system malignant tumor may exert a positive effect on the improvement of patients′ prognosis and have a referential significance to the clinical treatment.
		2019 Vol. 16 (23): 98-101 [Abstract] ( 190 ) HTML (1 KB) PDF (646 KB) ( 46 )

	102	Relationship between traditional Chinese medicine syndrome differentiation and RAD51 in stage ⅢB/Ⅳ non-small cell lung cancer patients before and after radiotherapy
		HUANG Qian1 XIE Fangwei2
		Objective To investigate the relationship between traditional Chinese medicine syndrome differentiation and serum levels of RAD51 in patients with stage ⅢB/Ⅳ non-small cell lung cancer (NSCLC) before and after radiotherapy. Methods From May 2015 to May 2018, 85 patients with stage ⅢB/Ⅳ NSCLC admitted to the Department of Oncology of No.476 Hospital of the Joint Logistics Team of PLA were selected. The syndrome differentiation of traditional Chinese medicine was carried out in these NSCLC patients before and after radiotherapy, and serum levels of RAD51 were measured by the enzyme-linked immunosorbent assay method. The changes of traditional Chinese medicine syndromes, clinical efficacy, occurrence of radioactive pneumonia, expression changes of RAD51 and the relationship between radiotherapy efficacy and RAD51 in NSLCL patients were compared. Results After radiotherapy, the percentage of NSLCL patients with syndrome of lung stagnation and phlegm and blood stasis, yin deficiency phlegm heat, spleen deficiency with damp phlegm had statistically significant differences compared with those before radiotherapy (P ＜ 0.05 or P < 0.01). The differences of clinical efficacy in different syndrome types were statistically significant (P ＜ 0.05). There was no statistically significant difference in the classification of radiation pneumonitis (P ＞ 0.05). After radiotherapy, the expression of RAD51 in patients with the syndrome of lung stagnation phlegm and blood stasis, yin deficiency phlegm heat, spleen deficiency with damp phlegm were significantly lower than those before radiotherapy, the differences were highly statistically significant (P ＜ 0.01). There was no significant difference in the expression of RAD51 in the patients with syndrome of deficiency of both qi and yin before and after radiotherapy (P ＞ 0.05). Conclusion Traditional Chinese medicine syndrome differentiation and RAD51 expression before and after radiotherapy are important for predicting the efficacy of NSCLC radiotherapy. The expression of RAD51 after radiotherapy can be used as the basis of traditional Chinese medicine syndrome differentiation. RAD51 and traditional Chinese medicine syndromes can complement each other to guide individualized radiotherapy. The expression of RAD51 after radiotherapy can be used as the evidence of traditional Chinese medicine syndrome differentiation. RAD51 and traditional Chinese medicine syndromes complement each other and serve as an effective way to guide individualized radiotherapy.
		2019 Vol. 16 (23): 102-105 [Abstract] ( 199 ) HTML (1 KB) PDF (693 KB) ( 105 )

	106	Clinical study of Bevacizumab combined with Paclitaxel Liposome and Tegafur Gimeracil and Oteracil Potassium in the treatment of medium and advanced gastric cancer
		XU Zhen ZUO Yun LU Weidong
		Objective To study the clinical effect of Bevacizumab combined with Paclitaxel Liposome and Tegafur Gimeracil and Oteracil Potassium in the treatment of medium and advanced gastric cancer. Methods Ninety patients with medium and advanced gastric cancer who were treated in the Oncology Department of Zhangjiagang First People′s Hospital from April 2012 to December 2017 were selected and divided into study group and control group according to different treatment methods, with 45 cases in each group. The control group was treated with Paclitaxel Liposome combined with Tegafur Gimeracil and Oteracil Potassium, while the study group was treated with Bevacizumab on the basis of the control group. The clinical efficacy, serum carbohydrate antigen 724 (CA724), carbohydrate antigen 199 (CA199) levels, adverse reactions, quality of life and immune function were compared between the two groups. Results The total effective rate of the study group (88.89%) was higher than that of the control group (68.89%), and the difference was statistically significant (P ＜ 0.05). The levels of CA724 and CA199 in the two groups after treatment were significantly lower than before treatment, and the differences were all highly statistically significant (all P ＜ 0.01). The levels of serum CA724 and CA199 in the study group were all significantly lower than those in the control group, and the differences were all highly statistically significant (all P ＜ 0.01). There were no significant differences in the incidence of leukopenia, nausea and vomiting, abnormal renal and liver function and alopecia between the two groups (all P ＞ 0.05). The Karnofsky performance score of the study group was significantly higher than that of the control group after treatment, and the difference was highly statistically significant (P ＜ 0.01). The levels of CD4+ and CD4+/CD8+ in the study group were significantly higher than those in the control group, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Conclusion Bevacizumab combined with Paclitaxel Liposome and Tegafur Gimeracil and Oteracil Potassium is effective in the treatment of medium and advanced gastric cancer. It is worth popularizing and applying.
		2019 Vol. 16 (23): 106-109,117 [Abstract] ( 340 ) HTML (1 KB) PDF (669 KB) ( 125 )

	110	Effect of 2-methoxyestradiol on proliferation, migration and radioresistance of nasopharyngeal carcinoma stem cells
		LIU Jieqiong CAO Yongqing LI Ke QI Hui
		Objective To investigate the effect of 2- methoxyestradiol (2-ME2) on proliferation, migration and resistance to radiotherapy in nasopharyngeal carcinoma stem cells. Methods Nasopharyngeal carcinoma CNE-2 cells were selected to construct nasopharyngeal stem cell model, they were randomly divided into 0 μmol/L group, 4 μmol/L group and 8 μmol/L group by using random number table method. The proliferation of cells in each group was detected by CCK-8 cell proliferation test, Transwell cell migration test was used to detect the cell migration in each group, clonogenic assay was used to detect cell radiotherapy resistance, Western blot method was used to detect the expression of nuclear factor-k-gene-binding (NF-κB) p65 and hypoxia inducible factor-1α (HIF-1α) protein. Results OD value of cells in 4 μmol/L group and 8 μmol/L group was significantly lower than that in 0 μmol/L group (P < 0.05), and that in 8 μmol/L group was significantly lower than that in 4 μmol/L group (P < 0.05). The number of perforating cells in 4 μmol/L group and 8 μmol/L group was significantly less than that in 0 μmol/L group (P < 0.05); the number of perforating cells in 8 μmol/L group was significantly less than that in 4 μmol/L group (P < 0.05). The colony-forming ability of cells in 4 μmol/L group and 8 μmol/L group after 0 and 8 Gy irradiation was significantly lower than that in 0 μmol/L group (P < 0.05); the colony-forming ability of cells in 8 μmol/L group after 0 and 8 Gy irradiation was significantly lower than that in 4 μmol/L group (P < 0.05). The expression of NF-κB p65 and HIF-1α in 4 μmol/L group and 8 μmol/L group was significantly lower than that in 0 μmol/L group (P < 0.05); the expression of NF-κB p65 and HIF-1α in 8 μmol/L group was significantly lower than that in 4 μmol/L group (P < 0.05). Conclusion 2-ME2 can inhibit the proliferation and migration of nasopharyngeal carcinoma stem cells, and reduce the radiation resistance, the mechanism may be related to the inhibition of HIF-1/NF-κB signal transduction pathway, it is worthy of further study.
		2019 Vol. 16 (23): 110-113,126 [Abstract] ( 180 ) HTML (1 KB) PDF (818 KB) ( 73 )

	114	Clinical effect of acupuncture combined with medicine in the treatment of insomnia caused by cancer in patients with non-small cell lung cancer after chemotherapy
		CHI Wencheng1 WANG Binbin2 YANG Fan2 JIANG Qinghui3 JIANG Jiakang1
		Objective To observe clinical effect of acupuncture combined with medicine in the treatment of insomnia caused by cancer in patients with non-small cell lung cancer after chemotherapy. Methods From June 2017 to June 2018, 110 patients with cancer-related insomnia after chemotherapy were selected from the Oncology Clinic of the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine. They were divided into treatment group (55 patients) and control group (55 patients) according to different treatment methods. Treatment group was treated with acupuncture combined with Tianwang Buxin Dan plus or minus prescription, while control group was treated with oral Estazolam Tablets. After 14 days, total effective rate, Pittsburgh sleep quality index (PSQI) and adverse reactions were observed. Results Total effective rate of treatment group was higher than that of control group (P < 0.05). There was significant difference in total PSQI score between two groups after treatment (P < 0.01). After treatment, PSQI scores and total scores of treatment group were lower than those before treatment, and the differences were statistically significant (P < 0.05 or P ＜ 0.01). The sleep time, sleep quality, sleep time, hypnotic drug application score and total score of control group after treatment were lower than those before treatment, and the differences were statistically significant (P < 0.05 or P ＜ 0.01). The incidence of adverse reactions in treatment group was lower than that in control group (P < 0.05). Conclusion Clinical effect of acupuncture combined with medicine in the treatment insomnia caused by cancer in patients with non-small cell lung cancer after chemotherapy is significant.
		2019 Vol. 16 (23): 114-117 [Abstract] ( 165 ) HTML (1 KB) PDF (695 KB) ( 79 )

	118	Analysis of composition of hospitalization expenses and its influencing factors in patients with colorectal cancer
		WANG Shuqiang1 ZHONG Wenzhou2
		Objective To explore the composition of hospitalization expenses of patients with colorectal cancer and analyze the related factors affecting hospitalization expenses, so as to provide basis for clinical control of hospitalization expenses and prevention and treatment of colon cancer. Methods The data of 1400 patients with colorectal cancer admitted to Haikou People′s Hospital of Hainan Province from January 2013 to January 2018 were collected, and the composition of hospitalization expenses of patients with colorectal cancer was summarized, and the influencing factors of hospitalization expenses were analyzed by variance analysis and multiple regression analysis. Results The hospitalization time of the 1400 patients with colorectal cancer was 21 days (median), and the hospitalization cost was 19 287.94 yuan (median). Due to price changes and other reasons increasing year by year, the hospitalized patients were mainly farmers, accounting for 64.86% (908/1400), among which the drug cost accounted for the largest proportion of 33.62%. The length of stay, self-paid medical treatment, operation, drug proportion, age, gender and infection in the hospital had significant influences on the hospital ization expenses, and the differences were statistically significant (all P ＜ 0.05). Among them, the length of stay had the greatest impact on the hospitalization expenses of colorectal cancer and the cost increased with the length of stay. Conclusion In the composition of hospitalization expenses, drugs account for a large proportion, so it is necessary to reasonably control the use of drugs, formulate effective management measures, and effectively adjust the influencing factors of hospitalization expenses to reduce the economic burden of patients.
		2019 Vol. 16 (23): 118-121 [Abstract] ( 642 ) HTML (1 KB) PDF (644 KB) ( 84 )

	122	Analysis of the expression and clinical significance of LncRNA LINC00319 expression in lung adenoarinoma utilizing TCGA datasets
		CHEN Shaoting CAO Pengju YANG Yang CHEN Falin
		Objective To investigate the expression and clinical significance of long non-coding RNA (lncRNA) LINC00319 in lung adenocarcinoma and its adjacent tissues. Methods The RNAseq data of 516 cases of lung adenocarcinoma tissues (tumor group) and 59 cases of paracancer tissues (para-cancer control group) and the corresponding clinical data of lung adenocarcinoma patients were downloaded from the cancer genome atlas (TCGA) database, and the expression of LINC00319 gene in the data set was analyzed and processed. The correlation between its expression and the clinicopathological features of lung adenocarcinoma and its effect on prognosis were analyzed and compared. Independent risk factors for prognosis of patients with lung adenocarcinoma were assessed by COX risk ratio model. Gene set enrichment analysis (GSEA) was used to predict the functional enrichment of LINC00319 molecular pathways. Results The expression level of LINC00319 in tumor group was significantly higher than that in the para-carcinoma control group (P ＜ 0.05). The expression level had no correlation with gender, age and N-staging (P ＞ 0.05), and significantly correlated with smoking, T-staging, M-staging and Stage grading (P ＜ 0.05). Survival analysis showed that high LINC00319 expression predicted worse prognosis in lung adenocarcinoma patients (P ＜ 0.05). The results of multiariable COX risk scale model showed that, high expression level, stage Ⅲ+Ⅳ and T3+T4 were independent risk factors for poor prognosis of patients with lung adenocarcinoma (P ＜ 0.05). In GSEA analysis, it was found that LINC00319 mainly enriched in cell adhesion, cytokine interaction, vascular endothelial growth factor signaling pathway, apoptosis and other pathways. Conclusion LINC00319 may promote the occurrence and development of lung adenocarcinoma through various ways, thereby affecting the prognosis and survival of lung adenocarcinoma patients. Therefore, LINC00319 can be used as a prognostic marker or potential therapeutic target for lung adenocarcinoma patients.
		2019 Vol. 16 (23): 122-126 [Abstract] ( 210 ) HTML (1 KB) PDF (1037 KB) ( 113 )

	127	Relationship between leukocyte differentiation antigen 147, vascular endothelial growth factor protein expression and clinical stage, lymph node metastasis in patients with lung adenocarcinoma
		YANG Jilan HU Fengdi XIE Lin LI Yang
		Objective To investigate the relationship between the expression of leukocyte differentiation antigen 147 (CD147) and vascular endothelial growth factor (VEGF) protein and clinical stage, lymph node metastasis in patients with lung adenocarcinoma. Methods A total of 78 patients with lung adenocarcinoma from October 2015 to October 2018 in the Third Affiliated Hospital of Kunming Medical University were enrolled. The lung adenocarcinoma tissues and corresponding normal lung tissues were taken and the lung glands were detected by immunohistochemical SP method. The expressions of CD147 and VEGF protein in cancer tissues and corresponding normal lung tissues were analyzed, and the relationship between CD147, VEGF protein expression and lymph node metastasis and clinical stage was analyzed. Results The positive expression rates of CD147 and VEGF protein in lung adenocarcinoma tissues were higher than those in corresponding normal lung tissues, the differences were statistically significant (P < 0.05). The positive expression rates of CD147 and VEGF protein in lung adenocarcinoma tissues with lymph node metastasis and clinical stage Ⅲ-Ⅳ were higher than those in non-lymph node metastasis and clinical stage Ⅰ-Ⅱ patients, the differences were statistically significant (all P < 0.05). Conclusion The expressions of CD147 and VEGF in lung adenocarcinoma are highly expressed, and their expression levels are related to lymph node metastasis and clinical stage. They can be used as an important index for the diagnosis and malignant degree evaluation of lung adenocarcinoma.
		2019 Vol. 16 (23): 127-130 [Abstract] ( 187 ) HTML (1 KB) PDF (683 KB) ( 129 )

	131	Relationship between serum level of IL-1β and MGP and cardiac valve calcification in maintenance hemodialysis patients
		CHEN Jie1 LUO Minhong2 CHEN Xingqiang1 ZHANG Meng1 FU Weiwei1
		Objective To study the relationship between serum level of interleukin-1β(IL-1β), matrix Gla protein (MGP) and cardiac valve calcification (CVC) in maintenance hemodialysis (MHD) patients. Methods Eighty patients who received MHD treatment in Sanya People′s Hospital of Hainan Province from January 2016 to November 2018 were selected as the study subjects and recorded as the observation group. Patients were divided into CVC group (n = 37) and non-CVC group (n = 43) according to whether they had CVC or not. Another 80 volunteers who received health examination during the same period were selected as the control group. The clinical data of the two groups was counted and compared. The clinical data of the subgroup in observation group was analyzed. The correlation between IL-1β, MGP and age, dialysis age was analyzed. The influencing factor of CVC in MHD patients were calculated by Logistic regression analysis. Results The ratio of CVC and the level of IL-1β in the observation group were higher those that in the control group, while the level of MGP was lower than that in the control group (P < 0.01). Age, dialysis age and IL-1β level in CVC group were higher than those in non-CVC group, while MGP level was lower than that in non-CVC group (P < 0.05 or P < 0.01). Pearson analysis showed that, IL-1β was positively correlated with age and dialysis age (r = 0.614, 0.679, all P < 0.05), while MGP was negatively correlated with age and dialysis age (r = -0.702, -0.715, all P < 0.05). Logistic regression analysis showed that, IL-1β≥160 pg/mL (OR = 1.244，95%CI: 1.021-4.820，P < 0.01), dialysis age≥30 months (OR = 1.319，95%CI: 1.039-10.512，P < 0.05) and MGP<2.55 ng/mL (OR = 1.108，95%CI: 1.007-11.287，P < 0.01) were risk factors for CVC in MHD patients. Conclusion Serum levels of IL-1β and MGP are closely related to CVC in MHD patients, and both of them and dialysis age are the influencing factors of CVC in MHD patients. Clinical monitoring of these indicator can better assess patient′s condition.
		2019 Vol. 16 (23): 131-134 [Abstract] ( 167 ) HTML (1 KB) PDF (568 KB) ( 24 )

	135	Clinical effect of pulse point penetrating needling on lumbar intervertebral disc herniation
		DENG Yue1 WANG Shiguang1 WANG Guiling2 MAO Xuewen1 ZHOU Xiaoping1 YANG Yi1 YAO Jin1 LI Mingna1
		Objective To observe clinical effect of pulse point penetrating needling on lumbar intervertebral disc herniation. Methods From June 2017 to April 2018, 80 patients with lumbar intervertebral disc herniation who met the inclusion criteria in outpatient department of acupuncture of Shunyi Hospital of Beijing Traditional Chinese Medicine Hospital were selected and randomly divided into observation group and control group with 40 cases in each group. Observation group was treated with pulse point penetrating needling method, while control group was treated with conventional needling method. Both groups were treated continuously for 4 weeks. Visual analogue scale (VAS) was used to assess the degree of pain before treatment, 2 weeks after treatment, 4 weeks after treatment and at follow-up. JOA scale was used to evaluate the improvement of low back pain. Clinical efficacy of two groups after 4 weeks of treatment was compared. Results VAS scores of two groups at 2, 4 weeks of treatment and follow-up were lower than those before treatment, and the differences were statistically significant (P < 0.05). VAS scores of observation group were lower than those of control group at 2, 4 weeks of treatment and follow-up, and the differences were statistically significant (P < 0.05). JOA scores of two groups at 2, 4 weeks of treatment and follow-up were higher than those before treatment, and the differences were statistically significant (P < 0.05). JOA scores of observation group were higher than that of control group at 2, 4 weeks of treatment and follow-up, and the differences were statistically significant (P < 0.05). After 4 weeks of treatment, the recovery rate of observation group was significantly higher than that of control group, and the difference was statistically significant (P < 0.05). Conclusion Puncture through pulse acupoints can relieve patients′ pain faster and improve the symptoms of low back pain, which is worthy of clinical promotion.
		2019 Vol. 16 (23): 135-138 [Abstract] ( 161 ) HTML (1 KB) PDF (602 KB) ( 141 )

	139	Treatment effect of emergency dispatch telephone guidance for cardiopulmonary resuscitation on patients with sudden prehospital death
		LI Liping XU Meiling WU Shuhong
		Objective To investigate the effect of emergency dispatch telephone guidance cardiopulmonary resuscitation on patients with sudden prehospital death. Methods A total of 143 cases of sudden death treated by Haikou 120 Emergency Center of Hainan Province ("our hospital" for short) from September 2017 to March 2018 were selected as observation group. Another 126 cases of cardiopulmonary resuscitation without emergency dispatch telephone guidance in our hospital from March to August 2017 were selected as control group. Observation group was given emergency dispatch telephone to guide cardiopulmonary resuscitation, while control group was not given medical guidance after receiving emergency telephone. The success rate, survival rate and self-care rate of cardiopulmonary resuscitation were compared between two groups. Results The success rate of cardiopulmonary resuscitation in observation group was significantly higher than that in control group, the difference was statistically significant （P < 0.05）. The survival rate and self-care rate of observation group were higher than those of control group, and the differences were statistically significant (P < 0.05). Conclusion Emergency dispatch telephone can improve the success rate and prognosis of CPR in sudden death patients. It can make up for the lack of knowledge of cardiopulmonary resuscitation before hospital, but because of the specialty, it can improve the prognosis of patients with sudden death. It still needs the joint efforts of the social sector to fundamentally improve the success rate of pre-hospital treatment.
		2019 Vol. 16 (23): 139-141,145 [Abstract] ( 129 ) HTML (1 KB) PDF (576 KB) ( 52 )

	142	Clinical effect of Xinmailong Injection in the treatment of sepsis combined with heart failure
		YANG Xixi CAO Dong▲ LIU Qian DENG Kai LIANG Qing TIAN Xin AN Hongming LI Lei
		Objective To analyze the clinical effects of Xinmailong Injection in the treatment of sepsis complicated with heart failure. Methods From December 2016 to November 2018, 100 patients with sepsis and heart failure admitted to the First Central Hospital of Baoding City in Hebei Province were selected as subjects. According to the principle of random number table, patients were divided into control group and observation group, 50 cases in each group. Patients in control group received anti-infective and anti-heart failure treatments. Patients in observation group received Xinmailong Injection on this basis. Both groups were treated for 7 days. Lee′s heart failure score, cardiac function index [Left ventricular ejection fraction (LVEF), cardiac output (CO), shortening fraction of left ventricle (SFLV), and stroke volume (SV)] were used to evaluate the degree of heart failure and cardiac function. The differences between the above indicators and PCT and BNP levels were compared between two groups. Results After treatment, Lee′s heart failure score in both groups was lower than that before treatment, and observation group was lower than that in control group, the difference was statistically significant (P < 0.05). After treatment, LVEF, CO, SFLV and SV of two groups were higher than those before treatment, and observation group was higher than control group, the difference was statistically significant (P < 0.05). After treatment, the serum PCT and BNP levels of two groups were lower than those before treatment, and observation group was lower than control group, the difference was statistically significant (P < 0.05). Conclusion Xinmailong Injection can effectively reduce the score of Lee′s heart failure in patients with sepsis and heart failure, improve heart function, and reduce serum PCT and BNP levels.
		2019 Vol. 16 (23): 142-145 [Abstract] ( 219 ) HTML (1 KB) PDF (603 KB) ( 104 )

	146	Analysis of clinical features and risk state of children with esophageal button-battery impaction
		DUAN Qingchuan WANG Guixiang WANG Hua LIU Zhiyong LIU Yuwei LI Hongbin YANG Xiaojian ZHAO Jing ZHANG Jie▲
		Objective To analyze the clinical features of 28 children with esophageal foreign body due to button-battery ingestion in order to guide the clinical work in these situation. Methods Clinical data of 28 children with esophageal foreign body due to button-battery ingestion in Department of Otorhinolaryngology Head and Neck Surgery of the Beijing Children′s Hospital, Capital Medical University from March 2016 to March 2019 were retrospectively analyzed, the age, impaction time, symptoms and complications of children were analyzed. Results There were 21 boys and 7 girls with a median age of 19 months were included, the median esophageal impaction time was 7.5 h. The most common symptoms were vomiting (10 cases) and dysphagia (9 cases). 24 cases (85.7%) were diagnosed with X-ray, while 3 cases (10.7%) were diagnosed with endoscopy, the other one was diagnosed with CT scan. Batteries of all children were taken out underwent surgery and given nasogastric tube until 2 to 4 weeks after sugery. The average time from chidren presentation to removal of the battery was 2 h (range of 1 to 5 h). Most batteries (19 cases, 67.9%) were impacted at the upper portion of the esophagus, followed by the lower portion (6 cases, 21.4%), and only 3 cases were at the middle portion. The median hospital stay was 7 d (range of 2 to 21 d). 3 cases (10.7%) had tracheoesophageal fistula. 2 cases of them recovered with effective treatment and one died. The median age and impaction time between children with and without complication had no statistics significant difference (P > 0.05). The median of hospital stay of children with complication was longer than the children without complication, the difference was highly statistically significant (P ＜ 0.01). Conclusion Children with esophageal button-battery impaction should be taken into emergent operation to get rid of the battery and indwelling gastric tube, close observation is necessary for monitoring and managing the complications.
		2019 Vol. 16 (23): 146-149,155 [Abstract] ( 144 ) HTML (1 KB) PDF (843 KB) ( 114 )

	150	Analysis of clinical efficacy, safety and prognosis of docetaxel combined with nedaplatin in local advanced nasopharyngeal carcinoma
		LIU Tongxin SUN Quanquan CHEN Jing LI Bin QIN Weifeng WANG Fangzheng FU Zhenfu HU Fujun
		Objective To evaluate the efficacy and safety of Docetaxel combined with Nedaplatin (TP) and Fluorouracil combined with Cisplatin (PF) in the treatment of locally advanced nasopharyngeal carcinoma. Methods Retrospective analysis of 271 patients who received TP or PF induction chemotherapy in Zhejiang Cancer Hospital from January 2012 to May 2014, 8 cases were lost to follow-up, and 263 cases were finally included in the analysis, including 92 cases in PF group and 171 cases in TP group. Efficacy, safety and prognosis of the two groups were compared. Results The 5-year overall survival rate (OS), progression-free survival rate (PFS), local recurrence free survival rate (LRFS), and distant metastasis free survival rate (DMFS) were compared, the differences were not statisticdly significant between the two groups (all P ＞ 0.05). Further multivariate analysis showed that N staging was an independent prognostic factor for 5-year OS, PFS and DMFS (P ＜ 0.05). Adverse reaction analysis showed that grade 3-4 leukopenia, neutrophilia and thrombocytopenia were more likely to occur in the TP group, while grade 3-4 nausea and vomiting were more likely to occur in the PF group, with statistically significant differences (P ＜ 0.05). There were no statistically significant differences in other adverse reactions between the two groups (P ＞ 0.05). Conclusion The efficacy of TP and PF induction chemotherapy in the treatment of locally advanced nasopharyngeal carcinoma is similar, and the adverse reactions are tolerable, which can be used in the treatment of locally advanced nasopharyngeal carcinoma.
		2019 Vol. 16 (23): 150-155 [Abstract] ( 182 ) HTML (1 KB) PDF (564 KB) ( 93 )

	156	Clinical observation of Cetuximab combined with Chemotherapy in the treatment of middle and advanced esophageal cancer
		LIU Zhijun HE Zhongliang CHEN Guoxing HE Xueming
		Objective To investigate the clinical observation of Cetuximab combined with Chemotherapy in the treatment of middle and advanced esophageal cancer. Methods Eighty patients with advanced esophageal cancer admitted to Tongde Hospital Affiliated to Zhejiang Chinese Medical University from February 2013 to May 2016 were selected as research objects. Patients were divided into control group (n = 40) and study group (n = 40) by random number table method. The control group was given Fluorouracil combined with cisplatin (FP) chemotherapy, and the study group was combined with Cetuximab on the basis of the conttol group. The clinical efficacy, tumor marker level, quality of life, survival rate and adverse reactions of the two groups were compared. Results The total clinical response rate of the study group was higher than that of the control group (P ＜ 0.05). After 4 cycles of treatment, SCC and CEA were decreased in both groups, and the study group were lower than those in the control group (P ＜ 0.05). EORTC QLQ-C30 scale score was increased in both groups after 4 cycles of treatment, and the group was higher than the control group (P ＜ 0.05). The survival rate of the study group was higher than that of the control group after follow-up of 12, 18 and 24 months, but there were no statistically significant difference between the two groups (P ＞ 0.05). There was no statistically significant difference between the two groups in the incidence of adverse reactions during treatment (P ＞ 0.05). Conclusion Cetuximab combined with Chemotherapy in the treatment of advanced esophageal cancer has a definite effect, can effectively improve the level of tumor markers, improve the quality of life of patients, without increasing the incidence of adverse reactions, and has a high clinical application value.
		2019 Vol. 16 (23): 156-159 [Abstract] ( 175 ) HTML (1 KB) PDF (418 KB) ( 144 )

	160	Influence of coronary heart disease patients by “internet plus+”health management intervention model
		CHENG Jing TANG Xiumei▲
		Objective To approach influence of coronary heart disease patients by “internet plus+” health management intervention model. Methods A total of 150 cases clinical data of coronary heart disease patients in Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medical, which were divided into two group by different intervention model, control group（regular management intervention model）70 cases and detection group （“internet plus+”health management intervention model）80 cases according to intervention patterns. The self-rating anxiety scale (SAS), self-rating depression scale (SDS), control attitude scale-R (CAS-R) and self-efficacy score before and after the intervention, and the total effective rate of the two groups were observed. Results The scores of SAS, SDS, CAS-R and self-efficacy of patients before intervention were compared between two groups, the differences were no statistically significance（P ＞ 0.05）. After intervention, SAS, SDS scores of two groups were lower than those before intervention, and CAS-R, self-efficacy scores were higher than before intervention; SAS, SDS scores of observation group after the intervention were lower than the control group, the CAS-R, self-efficacy scores were higher than the control group, the clinical total effective rate of observation group was higher than the control group, the differences were statistically significant (all P ＜ 0.05). Conclusion The application of coronary heart disease patients by “internet plus+” health management intervention model could improve bad mood, increase control attitude, self-efficacy and clinical result, it is worth to be promotion and application. [Key words] “Internet plus+” health management intervention model; Coronary heart disease; Self-rating anxiety scale; Self-rating depression scale; Control attitude scale; Self-efficacy score
		2019 Vol. 16 (23): 160-163 [Abstract] ( 163 ) HTML (1 KB) PDF (454 KB) ( 67 )

	164	Application effect of CICARE communication mode in the nursing of patients with chronic heart failure nursing
		WANG Yanting CHU Junxiao CAO Xuelian
		Objective To approach effect of CICARE communication mode in the nursing of patients with chronic heart failure nursing. Methods The clinical data of 100 patients with chronic heart failure admitted to the Department of Cardiology of Medicine Shijitan Hospital Affiliated to Capital Medical University from November 2017 to November 2018 were analyzed, according to random paper method, 50 cases were divided into the control group (traditional nurse-patient communication mode) and 50 cases were divided into the observation group (CICARE communication mode). Exercise tolerance, left ventricular ejection fraction (LVEF), plasma amino-terminal brain natriuretic peptide precursor (NT-proBNP), self-efficacy score, quality of life score and self-management score were observed before and after intervention in the two groups of patients. Results Before intervention, exercise tolerance, LVEF, NT-proBNP, self-efficacy score, quality of life score and self-management score of the two groups showed no statistically significant difference (all P ＞ 0.05). After intervention, exercise tolerance, LVEF, self-efficacy scores and self-management scores of the two groups were all higher than those before intervention, NT-proBNP and life quality scores were lower than those before intervention (P < 0.05). After intervention, exercise tolerance, LVEF, self-efficacy scores and self-management scores of the observation group were all higher than those of the control group, and its NT-proBNP and quality of life scores were lower than those of the control group; the total effective rate of the observation group was higher than that of the control group, and the differences were statistically significant (all P ＜ 0.05). Conclusion The application of CICARE communication mode in patients with chronic heart failure can improve patients′ cardiac function, enhance their self-efficacy and self-management level, and reduce the impact on patients′ quality of life, which is worthy of clinical promotion and application.
		2019 Vol. 16 (23): 164-167 [Abstract] ( 321 ) HTML (1 KB) PDF (462 KB) ( 183 )

	168	Professor Liu Jianqiu′s clinical experience in treating chronic obstructive pulmonary disease with syndrome of turbid phlegm obstructing lung by Modified Maxing Ersan Decoction
		WANG Xuehui1 MA Ying2 LI Zhuying1
		This paper introduces Professor Liu Jianqiu′s experience in treating chronic obstructive pulmonary disease with syndrome of turbid phlegm obstructing lung by Modified Maxing Ersan Decoction. Professor Liu believes that the pathogenesis of chronic obstructive pulmonary disease is the interaction between "vital qi deficiency" and "pathogen excess", both are involved in the development of disease. And for the treatment of this disease, Professor Liu also points out that we should first distinguish deficiency syndrome and reality syndrome of the disease. In most cases, the stable period of chronic obstructive pulmonary disease is usually characterized by deficiency syndrome, while in its acute attack stage, it is characterized by excess syndrome, and the occurrence of its "excess" syndrome is mostly based on the premise of "deficiency", the disease is mainly caused by phlegm turbid touching the deficiency of the original. Therefore, it is necessary to treat the "manifestation" first and then take into account the "root cause", so as to achieve the effect of ventilating lung qi for dissipating phlegm and descending qi to relieve asthma. Modified Maxing Ersan Decoction was made by Professor Liu according to the characteristics and the corresponding symptoms of this disease, combined with the efficacy and compatibility of drugs and years of clinical experience. It has been applied for many years and achieved satisfactory results, which is worthy of further exploration and application in clinic.
		2019 Vol. 16 (23): 168-171,179 [Abstract] ( 159 ) HTML (1 KB) PDF (680 KB) ( 95 )

	172	Research and exploration on undergraduate information literacy of traditional Chinese medicine universities based on scientific research needs
		JIA Hongtao1 LI Jingfei1 ZHAO Nan2 LENG Dayong1 LI Baohe1 ZHANG Yu1 CHE Zhiyuan3
		In the information age, people are endowed with abundant information resources. Today, with the rapid development of information technology, it is particularly important to master information literacy knowledge, strengthen information literacy awareness and enhance information literacy ability. Especially, the development of scientific research is inseparable from the guarantee of information literacy. From the perspective of scientific research demand, this paper analyzes the information literacy education of undergraduates in Chinese medicine universities. In view of the existing problems of undergraduates' scientific research information literacy, the corresponding education programs and training measures are put forward in combination with the actual situation so as to enhance undergraduates′ awareness of information awareness, information knowledge, information skills, information morality and information security, and to achieve the improvement of overall scientific research level and comprehensive ability of information literacy.
		2019 Vol. 16 (23): 172-175,184 [Abstract] ( 287 ) HTML (1 KB) PDF (669 KB) ( 80 )

	176	Research on the construction of doctor-patient conversation based on the perspective of socialinguistics
		LIU Qi XUE Yingli
		In recent years, the relationship between doctors and patients has become increasingly tense. Newspapers, TV news, Weibo and other places often see various contradictions and conflicts caused by the disharmony between doctors and patients, which seriously affects the harmonious development of social order. The doctor-patient relationship was established during the communication between doctors and patients. The doctor-patient conversation is a direct reflection of the doctor-patient relationship. At present, doctor-patient conversation has become a topic of extensive research in linguistics, medical management and sociology. This paper analyzes the study field of feasibility about doctor-patient conversation from the unique perspective of psychology in sociolinguistics. From the perspective of doctor-patient conversation, this paper discusses the relationship between sociolinguistics and doctor-patient conversation in detail, and further proposes the principle of doctor-patient conversation based on the perspective of sociolinguistics, which intended to effectively promote conversational communication between doctors and patients and achieve harmonious development of doctor-patient relationship.
		2019 Vol. 16 (23): 176-179 [Abstract] ( 184 ) HTML (1 KB) PDF (650 KB) ( 138 )

	180	Explorations and practices of the Labor Union of Beijing Municipal Administration of Hospitals in the new era
		ZHAO Hong
		Faced with new conditions and tasks, guided by the goal of training "healthy, happy and promising staff" and inspired by the philosophy of "political guidance, staff first as well as reform and innovation", the Labor Union of Beijing Municipal Administration of Hospitals focuses on organizational building, ideological guidance, skill enhancement, health care, vitality promotion as well as rights and interests guarantee, engaging staff of municipal hospitals in medical reforms so as to make contributions to the health improvement of local residents. And four working experiences were summarized, namely: insisting on taking "rely on the organization to win support" as the starting point of work, "focus on the central task and serve the overall interests" as the basic point of work, "serve the workers and unite them" as the foothold in the work, "advance with the times, stick to reform and innovation" as the focus of work. Striving to make the trade union a harbor for the hearts and minds, a cradle of growth, a strong reliance and a reliable family of the employees.
		2019 Vol. 16 (23): 180-184 [Abstract] ( 158 ) HTML (1 KB) PDF (661 KB) ( 46 )

	185	SWOT analysis of the human resources in a third-grade class-A traditional Chinese medicine hospital in Beijing
		XIANG Guoliang1 ZENG Jia1 XIE Pengcheng1 LIU Ying1 ZHANG Jiayi2 LIU Longtao1
		Under the background of deepening medical system reform and "healthy China", the importance of hospital human resource management has become increasingly prominent, and the scientificity of hospital human resource management has become an important factor influencing the improvement of hospital performance and long-term development. This paper takes a third-grade class-A hospital of traditional Chinese medicine in Beijing as the research object and uses SWOT analysis method to comprehensively analyze the advantages, disadvantages, opportunities and threats of human resource development in the sample hospital. On the premise of fully understanding and analyzing the current situation of human resources in hospitals, this paper proposes the development direction and key points of human resources in the third-grade class-A of traditional Chinese medicine hospitals under the current historical background, and promotes suggestions on promoting the development of human resource management and formulating human resource planning for third-grade class-A of traditional Chinese medicine hospitals from the four aspects of "selection, education, utilization and retention".
		2019 Vol. 16 (23): 185-188 [Abstract] ( 235 ) HTML (1 KB) PDF (700 KB) ( 28 )

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