利用TCGA数据集分析LncRNA LINC00319在肺腺癌中的表达及临床意义

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Abstract Objective To investigate the expression and clinical significance of long non-coding RNA (lncRNA) LINC00319 in lung adenocarcinoma and its adjacent tissues. Methods The RNAseq data of 516 cases of lung adenocarcinoma tissues (tumor group) and 59 cases of paracancer tissues (para-cancer control group) and the corresponding clinical data of lung adenocarcinoma patients were downloaded from the cancer genome atlas (TCGA) database, and the expression of LINC00319 gene in the data set was analyzed and processed. The correlation between its expression and the clinicopathological features of lung adenocarcinoma and its effect on prognosis were analyzed and compared. Independent risk factors for prognosis of patients with lung adenocarcinoma were assessed by COX risk ratio model. Gene set enrichment analysis (GSEA) was used to predict the functional enrichment of LINC00319 molecular pathways. Results The expression level of LINC00319 in tumor group was significantly higher than that in the para-carcinoma control group (P ＜ 0.05). The expression level had no correlation with gender, age and N-staging (P ＞ 0.05), and significantly correlated with smoking, T-staging, M-staging and Stage grading (P ＜ 0.05). Survival analysis showed that high LINC00319 expression predicted worse prognosis in lung adenocarcinoma patients (P ＜ 0.05). The results of multiariable COX risk scale model showed that, high expression level, stage Ⅲ+Ⅳ and T3+T4 were independent risk factors for poor prognosis of patients with lung adenocarcinoma (P ＜ 0.05). In GSEA analysis, it was found that LINC00319 mainly enriched in cell adhesion, cytokine interaction, vascular endothelial growth factor signaling pathway, apoptosis and other pathways. Conclusion LINC00319 may promote the occurrence and development of lung adenocarcinoma through various ways, thereby affecting the prognosis and survival of lung adenocarcinoma patients. Therefore, LINC00319 can be used as a prognostic marker or potential therapeutic target for lung adenocarcinoma patients.

Key words： Lung adenoarinoma Cancer genome atlas Long noncoding RNA LINC00319

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	CHEN Shaoting CAO Pengju YANG Yang CHEN Falin

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CHEN Shaoting CAO Pengju YANG Yang CHEN Falin. Analysis of the expression and clinical significance of LncRNA LINC00319 expression in lung adenoarinoma utilizing TCGA datasets[J]. 中国医药导报, 2019, 16(23): 122-126.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I23/122

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