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| Discussion on cellular and molecular mechanism of Fuzheng Huayu Recipe against myocardial fibrosis from the point of view of matrix metabolism |
| ZHU Ke1 LOU Lixia1 QI Yifei1,2 GAO Yonghong1 LI Chunhong1 GUO Qianqian1 WU Dandan1 ZHANG Dongmei1 |
| 1.Key Laboratory of TCM Internal Medicine, Ministry of Education, Dongzhimen Hospital of Beijing University of Tra Chinese Medicine, Beijing 100700, China; 2.Institute of Clinical Basic Medicine of Traditional Chinese Medicine, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China |
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Abstract Objective To observe the effect of Fuzheng Huayu Recipe (FZHY) on the extracellular matrix (ECM) metabolism of rat cardiac fibroblasts (CFs) induced by angiotensin Ⅱ (Ang Ⅱ), and to explore the mechanism of FZHY in treating myocardial fibrosis. Methods Differential adherent method was used to extract primary CFs from neonatal rats and subculture them. Cells can be divided into three groups, each group of 6 samples, add 2% FBS-DMEM (blank control group), 1 × 10-7 mol/L Ang Ⅱ (Ang Ⅱ group), 1 × 10-7 mol/L Ang Ⅱ + 100 μg/mL FZHY (FZHY group). Intervention after 48 h, collagentype Ⅰ(ColⅠ), ColⅢ, matrix metalloproteinases (MMP)-2, MMP-9 and TIMP-1 and TIMP-2 secretion were detected by the method of ELISA; ColⅠ, ColⅢ, MMP-2 and MMP-9 and TIMP 1 and TIMP-2 mRNA expression were detected by real-time fluorescent quantitative PCR method. Results Compared with blank control group, Col Ⅰ, Col Ⅲ content and mRNA expression of Ang Ⅱ group increased (P ＜ 0.01). Compared with Ang Ⅱ group, Col Ⅰ, Col Ⅲ content and its mRNA of FZHY group expression decreased (P ＜ 0.05 or P ＜ 0.01). Compared with blank control group, MMP-2, MMP-9 and TIMP 1 and TIMP-2 and its mRNA expression of Ang Ⅱ group increased (P ＜ 0.05). MMP-2/TIMP-2 and MMP-9/TIMP-1 showed a decreasing trend. Compared with Ang Ⅱ group, MMP-2, MMP-9 and TIMP 1 and TIMP-2 and its mRNA expression, and its MMP-2/TIMP-2 of FZHY group decreased (P ＜ 0.05 or P ＜ 0.01), and MMP-9/TIMP-1 had a tendency to decrease, but there was no statistical significance (P > 0.05). Conclusion FZHY can reduce the CFs ColⅠ, ColⅢ expression, its mechanism may be related to downgrade MMP-2, MMP-9 and TIMP 1 and TIMP-2 and its mRNA expression, reduce the MMP-2/TIMP-2, MMP-9/TIMP-1 ratio, regulating the metabolism of ECM.
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