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| Effect of Edaravone on acute lung injury in seawater drowning rats |
| CHEN Zhuo1 SHAN Qi1 LI Hong2 |
1.Logistics College of Armed Police, Tianjin 300309, China;
2.Department of Anesthesia, Special Medical Center of Armed Police Force, Tianjin 300162, China |
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Abstract Objective To observe the therapeutic effect of Edaravone on acute lung injury in seawater drowning rats and explore its mechanism. Methods Forty healthy male SD rats were divided into control group (group C), Edaravone group (group E), seawater drowning group (group S) and treatment group (group SE) according to random number table method, with 10 rats in each group. Artificial seawater was injected into trachea to establish animal models: group C was injected with 2 mL/kg saline via tail vein after tracheal exposure; group E was injected with 2 mL/kg Edaravone injection via tail vein after tracheal exposure; group S was injected with 4 mL/kg artificial seawater after tracheal exposure + 2 mL/kg saline via tail vein; group SE was injected with 4 mL/kg artificial saline via trachea after tracheal exposure + 2 mL/kg Edaravone injection via tail vein. Six hours after the establishment of the model, rats were killed. The wet/dry weight ratio, protein concentration in bronchoalveolar lavage fluid, interleukin (IL)-6, IL-10, serum tumor necrosis factor-α (TNF-α) content and pathological changes of lung tissue were detected. The mRNA and protein expression of aquaporin (AQP)-1 and AQP5 were detected by RT-PCR and Western blot. Results The wet-dry ratio of lung tissue in group S was significantly higher than that in group C (P < 0.05), and that in group SE was lower than that in group S (P < 0.05). The protein content of bronchoalveolar lavage fluid in group S was significantly higher than that in group C (P < 0.05); the protein content of bronchoalveolar lavage fluid in group SE was lower than that in group S (P < 0.05). The serum levels of IL-6, IL-10 and TNF-α in group S and SE were significantly higher than those in group C (P < 0.05); the serum levels of IL-6 and TNF-α in group SE were significantly lower than those in group S, and the serum level of IL-10 was significantly higher than that in group S (P < 0.05). The expression of AQP1 and AQP5 in lung tissue of rats in group S was higher than that in group C (P < 0.05); the expression of AQP1 and AQP5 in lung tissue of rats in group SE was lower than that in group S (P < 0.05). Conclusion Edaravone can alleviate the acute lung injury caused by seawater drowning by regulating the expression of AQP1 and AQP5.
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