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2019 Vol. 16, No. 28
Published: 2019-10-05
 
 
7 Studies of miR-361-3p in regulating the function of glioblastoma cells
GUO Xiaoye MAO Ping WANG Jia LIAN Haiping WANG Wei BAI Xiaobin SONG Jinning
Objective To study the role of miR-361-3p in glioblastoma (GB) cells. Methods LN299, U87MG and normal human astrocytes (NHA) were cultured. miR-361-3p mimic or inhibitor and MTCP1 siRNA (si-MTCP1) were transfected into U87MG cells, respectively. Gene expression was tested by RT-PCR and Western blot. Cell proliferation and apoptosis were measured by CCK-8 and Caspase3 kits, respectively. The binding of miR-361-3p and MTCP1 were detected by luciferase reporter assay. Results Relative to NHA, miR-361-3p levels were lower in LN299 and U87MG. Transfected with mimic, MTCP1 levels and the proliferation of U87MG cells were decreased, and the Caspase3 activity was increased, and luciferase activity in WT-MTCP1 was decreased. Furthermore, transfected with inhibitor, the proliferation of U87MG cells were increased, while the apoptosis rate in the si-MTCP1 group was increased. Conclusion MiR-361-3p may inhibit the proliferation and promote apoptosis of GB cells by inhibiting MTCP1.
2019 Vol. 16 (28): 7-11 [Abstract] ( 228 ) HTML (1 KB)  PDF (910 KB)  ( 177 )
12 Study on anti-HBV effect of total phenolics acid from Oenanthe Javanica in vitro and its mechanism
LIU Qingchuan1 WANG Panli2 YANG Xinbo1 WANG Renjie1 WANG Mengmeng1 HUANG Zhengming1
Objective To observe the inhibitory effect of total phenolics acid from Oenanthe Javanica on hepatitis B virus (HBV) in vitro and explore its mechanism. Methods The toxicity of total phenolics acid from Oenanthe Javanica to Hep AD38 cells was divided into the total phenolics acid from Oenanthe Javanica group (subdivided into 1600, 800, 400, 200, 100 μg/mL concentration groups) and the blank control group. Hep AD38 cells were cultured in vitro and the toxicity range of total phenolics acid from Oenanthe Javanica was determined by MTT assay. In vitro anti-HBV effects of total phenolics acid from Oenanthe Javanica were divided into the total phenolics acid from Oenanthe Javanica group (subdivided into 200, 100, 50 and 25 μg/mL concentration groups), the positive control group [Entecavir (ETV) 10 μmol/L] and the virus control group. Hep AD38 cells were treated with total phenolics acid from Oenanthe Javanica solution with a concentration below the non-toxic concentration for 6 consecutive days. The supernatant and cells were collected. The titer of HBsAg and HBeAg in the supernatant was detected by enzyme linked immune sorbent assay (ELISA). The intracellular HBV DNA load was detected by real-time fluorescent quantitative PCR. The effects of total phenolics acid from Oenanthe Javanica on duck hepatitis B virus (DHBV) DNA polymerase were divided into the total phenolics acid from Oenanthe Javanica group (subdivided into 400, 200, 100, 50 and 25 μg/mL concentration groups), the negative control group and the DHBV DNA polymerase control group. DHBV DNA polymerase was extracted from duck liver infected with duck hepatitis B virus, different concentrations of total phenolics acid from Oenanthe Javanica solution were given, and the effects of total phenolics acid from Oenanthe Javanica on DHBV DNA polymerase activity were detected by ELISA. Results The non-toxic concentration of total phenolics acid from Oenanthe Javanica in Hep AD38 cells was 400 μg/mL. Compared with the virus control group, HBsAg, HBeAg titer and HBV DNA load were significantly decreased in the 200, 100 and 50 μg/mL concentration groups of the total phenolics acid from Oenanthe Javanica group, the differences were highly statistically significant (P ＜ 0.01). Compared with the DHBV DNA polymerase control group, DHBV DNA polymerase activity was inhibited in the 400, 200, and 100 μg/mL concentration groups of the total phenolics acid from Oenanthe Javanica group, the differences were highly statistically significant (P ＜ 0.01). Conclusion Total phenolics acid from Oenanthe Javanicahas a good anti-HBV effect in vitro, and its mechanism includes inhibition of HBV DNA polymerase activity.
2019 Vol. 16 (28): 12-16,34 [Abstract] ( 234 ) HTML (1 KB)  PDF (1030 KB)  ( 29 )
17 Electron staining of myocardial amyloidosis by different preparation methods and tea instead of uranium
ZHAO Ranxu1 WEI Yingjie1▲ LI Li2 WANG Qingzhi2 LU Yang2 XU Hongyu2 YOU Yiwei2
Objective To test whether oolong tea extract can be used as an electronic stain instead of uranyl acetate (UA) for myocardial amyloidosis staining. In the process of staining, different staining methods were used and improve the preparation methods and compare their safety and efficiency. Methods A total of 40 cases of clinically suspected amyloidosis were collected from Fuwai Hospital of the Chinese Academy of Medical Sciences from January to December 2017, and 10 cases of cardiac tissue positive after pathological observation and special staining. The myocardial tissues were prepared by conventional encapsulation and improvement of resin 812, and then two kinds of different stains (3% uranyl acetate solution, 0.5% oolong tea extract) were applied to the ultrathin section samples under the conditions of counterstaining lead or not counterstaining lead, and two different staining methods (drip method, slice box staining) electronic staining, and then the differences were compared under a transmission electron microscope. Results Under the condition of repeated staining of lead, the results of staining of amyloid substance in myocardium with two kinds of electron staining agents and the improved preparation method were all satisfactory. The background of the slice was clean, the contrast was high and the obtained image was clear. Conclusion In the observation of myocardial amyloidosis, oolong tea extract with section box staining and the improved preparation method can more safely and quickly replace dioxide-uranium acetate for transmission electron microscope electron staining.
2019 Vol. 16 (28): 17-20 [Abstract] ( 154 ) HTML (1 KB)  PDF (933 KB)  ( 74 )
21 Effect of EZH2 on testicular ischemia-reperfusion injury in rats
ZHONG Yunping ZHANG Jianjian WANG Lei LIU Xiuheng▲
Objective To investigate the effects of EZH2 on testicular ischemia-reperfusion injury in rats. Methods Eighteen adult healthy male SD rats were randomly divided into control group, ischemia group and EZH2 inhibition group, 6 rats in each group. The free testicles were immediately repositioned and fixed after clockwise torsion of 720° in control group. The free testicles were rotated torsion of 720° and maintained for 2 h in ischemia group and EZH2 inhibition group. Before clockwising torsion, EZH2 inhibitor group was intraperitoneally injected with E2H2 inhibitor UNC1999 [50 mg/(kg·d)] for 3 days, while the control group and ischemia group were intraperitoneally injected with the phosphate buffer saline (PBS) of same volume. Rats in each group were sacrificed after 4 h of torsion and reposition, and the ischemic testicular tissues were obtained. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. Histopathoiogical changes and germ cell apoptosis indices (AI) were observed using hematoxylin-eosin staining and in situ terminal transferase labeling technique. The expressions of EZH2, NF-E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were detected using Western blot. Results Compared with control group, the activity of SOD and the expression of Nrf2 and HO-1 were decreased after reduction, the expressions of MD, AI and EZH2 were increased in ischemic group and EZH2 inhibitor group, the differences were statistically significant (all P < 0.05). Compared with ischemic group, the activity of SOD and the expression of Nrf2 and HO-1 were increased after reduction, the expressions of MDA, AI and EZH2 were decreased in EZH2 inhibitor group, the differences were statistically significant (all P < 0.05). Conclusion EZH2 plays an important role in testicular ischemia-reperfusion injury. EZH2 inhibitor exerts a beneficial effect of testicles torsion repositioned after ischemia-reperfusion injury. This effect may be achieved through enhancing Nrf2/HO-1 expression and inhibiting apoptosis.
2019 Vol. 16 (28): 21-24 [Abstract] ( 175 ) HTML (1 KB)  PDF (847 KB)  ( 122 )
25 Research on the correlation of AFP gene polymorphism with the susceptibility of hepatocellular carcinoma
HUANG Yuliang WU Junrong ZHAO Huiliu LIU Zhimin LIANG Yihua HUANG Zhaodong ZHU Bo▲
Objective To investigate the relationship between the single nucleotide polymorphisms of alpha fetal protein (AFP), serum AFP levels and the onset risk of primary hepatocellular carcinoma (HCC). Methods Two hundred and six patients in the Affiliated Tumor Hospital of Guangxi Medical University from July to December 2017 were enrolled, among which, 118 cases were HCC patients (HCC group), 88 cases were non-tumor patients (control group). The serum AFP levels were detected by chemiluminescence immunoassay, and the polymorphism of rs4024 and rs737241 of AFP gene was analyzed with SNaPshot technique. Results The frequency of genotype and allele at site rs737241 in the HCC group and control group had a significant difference (P ＜ 0.05). The risk of HCC in the subjects carrying GA or AA genotype was 0.543 times as big as the individuals carrying GG genotype (P = 0.037, OR = 0.543, 95%CI: 0.306-0.965). The onset risk of HCC in individuals carrying A allele was 0.573 times as big as those carrying G allele (P = 0.008, OR = 0.573, 95%CI: 0.379-0.865). The serum AFP levels were correlated with age, Barcelona clinic liver cancer (BCLC) stage and Edmondson-Steiner (EDSON) grade (P ＜ 0.05), the older the age, the lower the serum AFP levels (P = 0.001, OR = 0.172, 95%CI: 0.059-0.501); the higher the BCLC stage, the higher the serum AFP levels (P = 0.000, OR = 4.420, 95%CI: 2.003-9.752); the higher the EDSON grade, the higher the serum AFP levels (P = 0.033, OR = 2.335, 95%CI: 1.068-5.102). Conclusion The polymorphisms of rs737241 for AFP gene may be correlated with the genetic susceptibility of HCC. The serum AFP level may be correlated with the age, the development and deterioration of HCC.
2019 Vol. 16 (28): 25-29 [Abstract] ( 237 ) HTML (1 KB)  PDF (359 KB)  ( 90 )
30 Detection of macaamide，macaene and glucosinolate in Maca from different producing areas
LI Aimin1,2 ZHAO Kexin3,4 DANG Yanting2,3,4 LIU Ming3 CAO Mengsi3 WEN Lin2 LI Zijie1 GAO Xiaodong1▲
Objective To investigate the content of active ingredients and main influencing factors in Maca from different producing areas in China. Methods Samples of 40 Maca fresh fruit were collected from five regions in Yunnan and Sichuan provinces. The fresh Maca fruits was sliced, dried, crushed and sieved to obtain maca powder. The content of glucosinolates, macaenines and macaenes in Maca was determined by high performance liquid chromatography. The determination of glucosinolates was carried out by C18 chromatographic column (3.9 mm×150 mm, 5 microns)， acetonitrile-water was used as the mobile phase of the gradient elute, with the detection wavelength of 229 nm and the flow rate of 1.0 mL/min. The determination of macaene and macaamide was carried out by Waters XTerra C18 column (250 mm × 4.6 mm, 5 μm), acetonitrile-water (all containing 0.005% trichloroacetic acid) was as the mobile phase of the gradient elute, macaene with the detection wavelength of 210 nm and macaamide with the detection wavelength 273 nm and the flow rate of 0.6 mL/min. Results The content of macaene, macaamide and glucosinolate were respectively 49.72-204.36 mg/100 g, 10.22-66.73 mg/100 g and 256.56-936.65 mg/100 g. Correlation and significance analysis showed that the content of active ingredients in Maca was not related to color, but the origin. The results of correlation and significance analysis indicated that the content of active ingredients in Maca had nothing to do with the color of Maca(P ＞ 0.05). The content of active ingredients in Maca in Sichuan Province and other areas of Yunnan Province was higher than that in Kunming(P ＜ 0.05). Conclusion The content of macaamide and macaene in different producing areas showed a positive correlation with altitude, but has nothing to do with the color of Maca fruit. The origin is also an important factor affecting the active ingredients of Maca.
2019 Vol. 16 (28): 30-34 [Abstract] ( 215 ) HTML (1 KB)  PDF (736 KB)  ( 287 )
35 Quantitative determination of seven components of Fufang Ganmaoling Fluid Extract by HPLC
LI Ke1 CHEN Dan2 LI Jun3 HOU Xi1 QI Jianzhong3 LI Ruocun1
Objective To establish simultaneous determination of 7 components in Fufang Ganmaoling Fluid Extract method by high performance liquid chromatography. Methods Kromasil C18 column（4.6 mm×250 mm, 5 μm） column was used as the column, acetonitrile- 0.4% phosphoric acid solution was used as the mobile phase of the gradient elute, with the detection wavelength of 327 nm and the flow rate of 1.0 mL/min. The colomn temperature maintained at 30℃. Results The neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 4,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, in the range of 5.681-113.62 μg/mL (r = 0.9996), 6.42-128.4 μg/mL (r = 0.9995), 6.806-136.12 μg/mL (r = 0.9996), 3.924-78.48 μg/mL (r = 0.9996), 4.863-97.26 μg/mL (r = 0.9994), 2.273-45.46 μg/mL (r = 0.9991), 43.08-861.6 μg/mL (r = 0.9993) showed good linear relationship with the peak area. The average recovery rates (n = 6) of the seven components were 97.79%, 98.24%, 96.92%, 97.84%, 99.23%, 99.00% and 100.04% respectively. Conclusion The method could be used for the comprehensive quality control of Fufang Ganmaoling Fluid Extract.
2019 Vol. 16 (28): 35-38,43 [Abstract] ( 218 ) HTML (1 KB)  PDF (667 KB)  ( 36 )
39 Rapid identification research on Angelica sinensis from different producing areas based on electronic nose technology
GONG Jianting1,2 ZOU Huiqin3 WANG Jiayu4 WU Haozhong3 WANG Daqian1,2 LI Jiahui3 LIU Changli5 LI Li1,2
Objective To establish a fast, accurate and reliable method for identification of Angelica sinensis of different origin, based on the odor fingerprint of Angelica sinensis of different origin, and the discrimination model was established based on the difference odor. Methods Odor analysis of Angelica sinensis samples from different habitats was carried out by electronic nose. The maximum, average, maximum slope and integral values of sensor signals were extracted as characteristic parameters. Principal component analysis and support vector machine were used to analyze the characteristic parameters. The discriminant model was established. And examine the identification effect of combination characteristics on Angelica sinensis from different habitats. Results Principal component analysis could not distinguish the origin of Angelica sinensis. Support vector machine could quickly and accurately identify the habitat of Angelica sinensis. When a single feature was used as a feature parameter, the identification effect was general, and the average positive detection rate on the test set was 86.03%. When combined features were adopted as the feature parameters, the effect was better. The average positive detection rate of the combination of two features and three features was 86.76% and 89.71% respectively, and the positive detection rate of the combination of four features was the highest, which was 91.18%. Conclusion Electronic nose technology can accurately identify Angelica sinensis samples from different regions, providing new technologies and methods for rapid identification of the origin of traditional Chinese medicine. The selection and optimization of different pattern recognition algorithms and characteristic parameters provide more ideas for the application of electronic nose data mining in traditional Chinese medicine research.
2019 Vol. 16 (28): 39-43 [Abstract] ( 152 ) HTML (1 KB)  PDF (934 KB)  ( 65 )
44 Research progress in the treatment of IgA nephropathy by Chinese and Western medicine
FAN Peipei1 TIAN Yun2
IgA nephropathy is a common primary glomerular disease in China, with a high incidence. Its etiology and pathogenesis are not yet clear, and there is no unified treatment plan at present. Drugs commonly used in Western medicine for IgA nephropathy include renin-angiotensin system blockers, glucocorticoids, immunosuppressants and so on. In the treatment of IgA nephropathy, traditional Chinese medicine carries out individualized differentiation of symptoms and signs on the basis of stages and types, and combines with personal experience, the clinical effect is remarkable. In the current situation of integrated medicine, when the advantages of Chinese and Western medicine in the treatment of IgA nephropathy are given full play and combined treatment is carried out. This paper reviews the research progress in the treatment of IgA nephropathy with Western medicine and traditional Chinese medicine.
2019 Vol. 16 (28): 44-47 [Abstract] ( 209 ) HTML (1 KB)  PDF (618 KB)  ( 145 )
48 Research progress on correlation between CD4+ new subsets of Th9 cells and common autoimmune diseases
ZHANG Huining1 WANG Jiajun2
Th9 cells are a newly discovered subset of differentiated CD4+T cells that participate in the body′s immune response by secreting interleukin-9 (IL-9). In patients with autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus, the proportion of Th9 cells and the content of IL-9 in the blood and tissues will change greatly, indicating that it is closely related to the occurrence and development of autoimmune diseases. This paper reviews the research progress on the correlation between Th9 cells and common autoimmune diseases in recent years.
2019 Vol. 16 (28): 48-51 [Abstract] ( 157 ) HTML (1 KB)  PDF (587 KB)  ( 158 )
52 Tumor immune escape from NK cells and immunomodulatory effects of traditional Chinese medicine on NK cells
SUN Yufang NI Zhongya WANG Lixin ZHU Shiguo
Malignant tumers are one of the diseases with the highest morbidity and mortality in the world. In recent years, tumor immunotherapy as a targeted therapy using its own immune system to induce anti-tumor effects has attracted more and more attention. Natural killer (NK) cells are lymphoid cells with a variety of immunological functions. They are important members of natural immunity. NK cells can directly or indirectly kill tumor cells through cytotoxicity and immune regulation, and play an important role in immune surveillance. However, there are many mechanisms in many malignant tumors to help the tumor escape the recognition and killing of NK cells, leading to immune escape. Therefore, how to restore and enhance the anti-tumor effect of NK cells is particularly important. Traditional Chinese medicine can regulate a variety of immune cell functions in the treatment of malignant tumors. This article reviews how tumor cells escape NK cells immune surveillance and the regulation of NK cells by traditional Chinese medicine.
2019 Vol. 16 (28): 52-55 [Abstract] ( 165 ) HTML (1 KB)  PDF (587 KB)  ( 167 )
56 Research progress of Keap1-Nrf2/ARE antioxidant pathway in tumors
YUN Tuya SU Xiulan
Reactive oxygen species can cause cell mutation and induce cell carcinogenesis by attacking deoxyribonucleic acid or ribonucleic acid. Keap1-Nrf2/ARE is an important oxidative stress pathway in the body. As the main effector of this system, Nrf2 can prevent malignant transformation of normal cells and play an anti-cancer role by activating and regulating the expression of various phase Ⅱ detoxifying enzymes in the body. Cellular and animal experiments have shown that Nrf2 can promote the development of tumors and increase the tolerance of cancer cells to chemotherapeutic drugs. Research on signal transduction of Keap1-Nrf2/ARE pathway is essential for cancer prevention, development and targeted therapy.
2019 Vol. 16 (28): 56-58,70 [Abstract] ( 362 ) HTML (1 KB)  PDF (602 KB)  ( 96 )
59 Progress in the role of autophagy in the pathogenesis of rheumatoid arthritis
QIN Qian1 QING Yufeng2 ZHOU Wenjun2 WANG Dan2 XIONG Qin3 YANG Xiaohong1，4
Rheumatoid arthritis is a chronic, inflammatory autoimmune disease, its pathogenesis is not yet fully clear. Autophagy widely exists in eukaryotes. It is a metabolic process that maintains normal physiological activity and homeostasis in cells. Its abnormalities are related to the occurrence and development of many diseases. In recent years, autophagy has attracted wide attention and become one of the research hotspots. Autophagy can affect the occurrence and development of rheumatoid arthritis in many ways. This article reviews this aspect.
2019 Vol. 16 (28): 59-62 [Abstract] ( 182 ) HTML (1 KB)  PDF (585 KB)  ( 81 )
63 Research progress of Pemetrexed nanoformulation
LING Jianxin1,2,3 NING Qian2 TANG Shengsong1,2
Pemetrexed (PEM) is a new generation of anti-tumor drug that blocks multiple targets of folate metabolism pathway, and it is more effective than other traditional anti-folate drugs. However, due to the lack of tumor specificity and dose-dependent toxicity, its clinical application is limited. Nanocarriers have attracted wide attention because of their unique advantages in reducing toxicity and improving therapeutic effect. In recent years, studies on PEM nanoparticles, including liposomes, nanoparticles, micelles, nanoemulsions and hydrogels, have made some progress in improving the solubility of PEM, prolonging the circulation time in vivo, improving targeting, reducing adverse reactions and enhancing anti-tumor activity, and different drug delivery methods have been developed. This paper reviews the research progress of PEM nanopreparation, and provides the theoretical basis for the development and application of new PEM nanopreparation.
2019 Vol. 16 (28): 63-66 [Abstract] ( 180 ) HTML (1 KB)  PDF (586 KB)  ( 143 )
67 Updates on neuroprotective effects and mechanisms of neuroglobin in epilepsy
DU Huan1 MI Man2 GUO Xiangyang3 LIU Manling2 LIU Na3
This study reviews and discusses the biological functions of neuroglobin and the neuroprotective effects of neuroglobin in epilepsy and its specific mechanisms. Neuroglobin is mainly involved in the proliferation of neural stem cells, promoting axonal regeneration and reducing oxidative stress levels, regulating apoptosis and autophagy pathways, thereby alleviating acute neuronal damage. The specific mechanism of neuroprotection of neuroglobin in epilepsy is regulating the Pik3k/Akt signaling pathway, the Jak2/Stat3 pathway, and the inhibition of activation of caspase-3 activity, and cooperating with the erythropoietin receptor to initiate the relevant signal transduction pathway. This review provides new ideas for preventing the death of neuronal cells caused by epilepsy and reference for treating brain damage caused by epilepsy.
2019 Vol. 16 (28): 67-70 [Abstract] ( 196 ) HTML (1 KB)  PDF (588 KB)  ( 146 )
71 The application value of Bayes′ theorem in diagnostic clinical reasoning training
DONG Jiaqiang1 WANG Rui2 ZHANG Wenyao3
Clinical diagnostic thinking is a process of making diagnostic decisions based on the collection and analysis of diagnostic clues. In the traditional teaching of diagnostics, the collection of diagnostic evidence is often emphasized, while the training of diagnostic evidence analysis and diagnosis decision-making is neglected. From the perspective of cognition, diagnostic analysis and decision-making involve complex neuropsychological activities and accumulation of experience, which often have significant individual differences. Therefore, it is of great significance to add the theory teaching of evidence analysis and diagnosis decision formation in diagnosis teaching to improve the training efficiency of diagnosis thinking. This paper analyzes the application value of bayesian statistics in diagnosis analysis and decision making.
2019 Vol. 16 (28): 71-74 [Abstract] ( 287 ) HTML (1 KB)  PDF (677 KB)  ( 143 )
75 Thinking about 3D reconstruction technique based on MIMICS software in teaching application of Dens Invaginatus in pediatric dentistry
WU Li′an ZHOU Zilin BAI Yudi GAO Lei ZHANG Caidi
Objective To explore the teaching efficiency and quality of Dens Invaginatus in pediatric dentistry using a three-dimensional reconstruction technique based on MIMICS software. Methods The three-dimensional reconstruction technique based on MIMICS software could present the digitized three-dimensional model of an abstract anatomy of dens invaginatus to the dental undergraduates, the multiangle and stereoscopic inside structure was observed with breaking through the limitation of plain vision, and easy to understand and master. The teaching effect of the undergraduates was evaluated after class and compared with the one using traditional teaching manner. Results The understanding of dens invaginatus of the undergraduates was improved obviously using this three-dimensional reconstruction technique based on MIMICS software. Conclusion This technique can effectively enhance the teaching efficiency and quality, which is easy to manipulate and master.
2019 Vol. 16 (28): 75-78 [Abstract] ( 209 ) HTML (1 KB)  PDF (682 KB)  ( 41 )
79 Application of advanced simulation human in clinical skills training of specialized resident doctors in obstetrics and gynecology
YAN Ping Li Hongyu YAN Xiaoli YU Meijia ZENG Xiaoqian CHANG Qing WANG Dan▲
Obstetrics and gynecology is a clinical subject with strong specialty and high requirement of clinical skills, but conventional situational simulation teaching methods can not fully meet the needs of clinical skills training for its main operation and surgical treatment relates to female concealed sites. Simulated teaching training based on advanced simulation human can not only make up for the shortcomings mentioned above, but also set up individualized case, stimulate students′ interest in clinical skills learning, improve the teaching quality of clinical thinking ability and operational skills.
2019 Vol. 16 (28): 79-81,90 [Abstract] ( 172 ) HTML (1 KB)  PDF (684 KB)  ( 152 )
82 Exploration and practice of "three-stage" guidance based on mobile terminal in medical microbiology teaching
LIU Zhixin YU Chunfang WANG Jingjie XIAO Xiao LI Moran YANG Jing JIN Zhixiong LIU Long▲
Objective To explore the application effect of "three-stage" guidance in medical microbiology teaching. Methods From March to June 2018, 56 students came from two clinical medicine classes of Hubei University of Medicine "our school" for short in 2017 were randomly selected as experimental groups, and the internet tools such as WeChat group, rain classroom and tencent were used to conduct "three-stage" guidance before, during and after class, on the basis of traditional teaching. Fifty-eight students who majored in clinical medicine came from two classes of our school in 2017 were randomly selected as the control group, the traditional teaching methods and multimedia teaching methods were used, the pre-study content was arranged before class, and the relevant review content and homework were finished after class. Students of both groups had the same curriculum background before observation, and the two groups also offered courses with the same name and explained by the same teacher, and also had the same extracurricular tasks in other courses. Through the  daily assessment, final assessment, questionnaire survey, expert group assessment, the teaching effect, teaching satisfaction, teaching process and student achievement of "three-stage" guidance were evaluated. Results The scores of daily assessment theoretical results and the total score of final examination of students in experimental group were higher than those in the control group, the differences were statistically significant (P < 0.05). The satisfaction rate of students in the experimental group was higher than that in the control group, the difference was statistically significant (P < 0.05). And teaching experts were satisfied with the teaching method of "three-stage" guidance. Conclusion The use of mobile terminals for "three-stage" guidance at pre-class, during-class and after-class can stimulate students′ learning interest, and improve the teaching effectiveness of medical microbiology.
2019 Vol. 16 (28): 82-85 [Abstract] ( 234 ) HTML (1 KB)  PDF (672 KB)  ( 88 )
86 Logical expression and structural analysis of thinking mode in clinical diagnosis
WANG Yuehua WANG Yajun LIU Dongbin ZHAO Jing MA Shenghai
By analyzing the syllogism rules of clinical diagnosis, the logical expression and structural diagnosis process of clinical thinking mode is constructed. The thinking process of clinical diagnosis is a combination of vertical and lateral thinking analysis and judgment process. The thinking mode in clinical diagnosis can be represented by a logical expression of circular judgment, the diagnostic process can be represented by a structured diagnostic flow chart. Taking acute abdomen as an example, the structural flow chart of the established thinking model can clearly indicate the process of patient data collection and the process of deductive reasoning in syllogism and the relationship between them. The establishment of the thinking mode in clinical diagnosis is helpful to the design of teaching course and teaching method of clinical thinking. It is also helpful to the cultivation of the ability of clinical thinking in the process of transforming medical students into clinicians.
2019 Vol. 16 (28): 86-90 [Abstract] ( 202 ) HTML (1 KB)  PDF (883 KB)  ( 183 )
91 Exploration of “double teaching and research section” in the construction of higher vocational pharmacy curriculum system reform
LIU Yuanfen
The reform of the curriculum system is the core of major construction. Focusing on the development of the pharmaceutical industry, building a proper curriculum system is of great significance to professional construction and personnel training. However, the curriculum system that emphasizes vocational ability training has encountered difficulties, such as limited teachers and limited resources. In order to promote the reform of the curriculum system, this paper implements the establishment of the curriculum system of the “double teaching and research section” of schools and enterprises (hospitals), and summarizes the relevant mechanisms to guarantee the implementation of the “double teaching and research section”. Practice has proved that the establishment of the “double teaching and research section” has promoted the opening of courses in the curriculum system with strong practical skills, the preparation of teaching materials for school-enterprise cooperation, the enthusiasm of cooperation between enterprises (industries), and the construction of curriculum teaching reform projects.
2019 Vol. 16 (28): 91-94,102 [Abstract] ( 147 ) HTML (1 KB)  PDF (770 KB)  ( 59 )
95 Research on the application of “flip classroom” teaching mode in teching infectious diseases integrated Chinese and Western medicine
GAO Lijuan1 ZHANG Yingying1 LI Zeguang1 ZHANG Chunfang2 JIANG Ning1 ZHENG Nan3
With the advent of the “Internet +” era, the traditional medical teaching model can not fully satisfy the current teaching situation, especially in the teaching of infectious diseases between Chinese and Western medicine, it is necessary to combine the flipped classroom teaching mode to meet the needs of personnel training. In this paper, the flipped classroom mode is applied to the undergraduate teaching of infectious diseases integrated Chinese and Western. Through the evaluation of student satisfaction and the comparison of the average scores of four consecutive students, the advantages of flipped classroom mode in the teaching of infectious diseases integrated Chinese and Western medicine are fully explained, and at the same time put forward the requirements for both teachers and students. It will lay a solid foundation for the cultivation of clinical and practical medical talents.
2019 Vol. 16 (28): 95-98 [Abstract] ( 204 ) HTML (1 KB)  PDF (671 KB)  ( 40 )
99 Practice and thinking on the mode of combining situational simulation and case discussion in the clinical teaching of standardized training for TCM resident
YU Lu
With the implementation and deepening of the standardized training policy for TCM residents, clinical teaching is especially important for strengthening the teaching quality and cultivating high-level talents of Chinese and Western medicine. The mode of combining scenario simulation and case discussion is a relatively new teaching method. Compared with traditional clinical teaching, it pays more attention to the reform of ideas, enrichment of contents and updating of methods. This teaching mode comprehensively optimizes the clinical teaching program of integrated Chinese and Western medicine, and gives full play to the purpose of teaching and learning and paying equal attention to each other. This paper aims at the common problems in the standardized training of TCM residents in clinical teaching, and focuses on the characteristics and advantages of the combination of scenario simulation and case discussion in clinical teaching to carry on thinking and discussion, including scene reproduction, easy teaching; guidance-based; guidance first, instillation second; promotion theory, combine practice.
2019 Vol. 16 (28): 99-102 [Abstract] ( 229 ) HTML (1 KB)  PDF (670 KB)  ( 32 )
103 The correlation between salivary macrophage migration inhibitory factor levels and primary Sjögren′s syndrome
QIANG Lu WANG Yumeng PEI Tingting
Objectives To detect salivary macrophage migration inhibitory factor (MIF) level in patients with primary Sjögren′s syndrome (pSS) and investigate the correlation between MIF level and disease activity. Methods The pSS patients diagnosed in Beijing University of Chinese Medicine Third Affiliated Hospital (hereinafter referred to as "our hospital") from October 2009 to October 2018 were enrolled as pSS group (96 cases); the other age- and sex- matched healthy people were included as control group (70 people) during the same period of our hospital. The disease activity of patients with pSS were evaluated, and the salivary MIF level of each group were compared. The relationship between salivary MIF level and the incidence of pSS was evaluated, while the correlation between saliva MIF levels and EULAR Sjogren′s syndrome disease activity index (ESSDAI) were analyzed. Results The salivary MIF level in the pSS group was significantly higher than that in the control group, and the differences were highly statistically significant (P ＜ 0.01). Salivary MIF level was the independent risk factors for pSS [OR（95%CI）=1.996（1.339-2.977），P ＜ 0.01]. Salivary MIF level was positively correlated with ESSDAI (r = 0.276，P ＜ 0.01). Conclusion Salivary MIF level is expected to be an important biomarkers for predicting the development of pSS.
2019 Vol. 16 (28): 103-105,117 [Abstract] ( 214 ) HTML (1 KB)  PDF (632 KB)  ( 71 )
106 Application effect of comprehensive management in chronic kidney disease patients in stage 3-4
ZHANG Heping1 XU Dan1 YUE Yanlin1 ZHANG Jie1 LIU Jiali1 WANG Xiaohong2 ZHAO Zongyang3 LIAO Huawei1▲
Objective To investigate the application effect of comprehensive management in patients with stage 3-4 chronic kidney disease. Methods Eighty-one patients with chronic kidney disease in stage 3-4 were selected from Affiliated Hospitals of North Sichuan Medical College, Kangqiao Hospital of Xichong Country and Nanchong Youhao Hospital from March 2016 to February 2018. The patients were randomly divided into the control group (40 cases) and the observation group (41 cases) by lottery, and they were treated with routine management and comprehensive management respectively. The differences in management effects between the two groups were observed for 3 months. Results The scores of diet management, treatment management, somatic activity management and psychosocial management in the observation group were higher than those in the control group, and the differences were statistically significant (P ＜ 0.05). After 3 months of management, the proportion of nutritional normal patients in the observation group were significantly higher than those in the control group, while the level of serum uric acid was lower than that in the control group, and the differences were statistically significant (P ＜ 0.05). Conclusion The application of comprehensive management in patients with chronic kidney disease in stage 3-4 is very effective. It can significantly improve the nutritional status of patients and reduce the level of serum uric acid, while enhance the ability of disease management. It is worthy to promote.
2019 Vol. 16 (28): 106-109 [Abstract] ( 158 ) HTML (1 KB)  PDF (544 KB)  ( 95 )
110 Clinical effect of autologous platelet-rich blood plasma gel combined with skin flap transplantation in the treatment of refractory wound surface
TIAN Meng1 WANG Gangren2 XU Xiaojing3 ZHANG Yingchuan4 ZHU Xiaohai1
Objective To investigate the effect of autologous platelet-rich blood plasma gel combined with skin flap transplantation in the treatment of refractory wound surface. Methods One hundred and twenty patients with refractory wound surface admitted to Changzheng Hospital Affiliated to The Second Military Medical University from May 2017 to May 2018 were selected and divided into the observation group and the control group according to the random number table method, with 60 patients in each group. The observation group was treated with autologous platelet-rich gel combined with skin flap transplantation in the treatment of refractory wound surface, while the control group was treated with conventional methods. After treatment, patients in the two groups were compared with dressing replacement and the number of dressing changes within one week, average wound surface healing rate, survival rate after skin flap transplantation, incidence of complications and the recurrence rate, inflammatory factor level in the wound, the degree of wound surface pain and scar injury before and after treatment. Results The number of wetted gauze layers, inner gauze replacement and dressing changes in the observation group within one week were significantly lower than those in the control group (all P ＜ 0.05). The average wound surface healing rate and skin flap survival rate in the observation group were significantly higher than those in the control group (all P ＜ 0.05). However, the incidence of complications, recurrence rate and the levels of inflammatory factor tumor necrosis factor α, interleukin-1β and γ-interferon were significantly lower in the observation group than those in the control group (all P ＜ 0.05). At the same time, the score of wound surface pain after treatment and scar injury during the 2 monthes of follow-up in the observation group was lower than those in the control group (all P ＜ 0.05). Conclusion The application of autologous platelet-rich blood plasma gel combined with skin flap transplantation in the treatment of refractory wound surface has a good therapeutic effect, which can significantly improve the wound healing rate, reduce the pain of patients, and improve the quality of life of patients. Therefore, it is worthy of clinical application.
2019 Vol. 16 (28): 110-113 [Abstract] ( 191 ) HTML (1 KB)  PDF (589 KB)  ( 83 )
114 Clinical effect of massage combined with deep spine popliteus in the treatment of patellofemoral pain syndrome
LI Hai ZHANG Hanyuan ZHANG Yuan QI Wei
Objective To investigate the effect of massage combined with deep spine popliteus in the treatment of patellofemoral pain syndrome. Methods Sixty-four patients with patellofemoral pain syndrome admitted to the Third Affiliated Hospital of Changchun University of Chinese Medicine from January to June 2018 were selected. According to the random number table, they were divided into the treatment group and the control group, 32 patients in each group. Patients of two groups were received rehabilitation training. Patients of the treatment group were received ring push patella and deep spine popliteus treatment on the basis of rehabilitation training. Three months after treatment, the pain degree and knee joint function of patients were evaluated by visual analogue scales (VAS), Lysholm knee joint function score scale (Lysholm), the international knee documentation committee knee evaluation form (IKDC) and Tegner knee exercise level score (Tegner). The therapeutic effects of the two groups were compared. Results After 3 months of treatment, VAS scores in the treatment group were significantly lower than that before treatment, IKDC scores were better than that before treatment, Lysholm scores and Tegner scores were significantly higher than those before treatment, the differences were statistically significant (all P ＜ 0.05); VAS in the treatment group were significantly lower than those in the control group after 3 months of treatment, while Tegner scores and Lysholm scores were significantly higher than that in the control group, the differences were statistically significant (all P ＜ 0.05). The IKDC scores of the treatment group was superior to that of the control group after 3 months of treatment, but the difference between the two groups was not statistically significant(P ＞ 0.05). Conclusion Massage combined with deep spine popliteus in the treatment of patellofemoral pain syndrome can effectively improve the cure rate of pain, with more significant clinical efficacy, which is of great significance in improving the knee joint function of patients and is worthy of clinical promotion.
2019 Vol. 16 (28): 114-117 [Abstract] ( 160 ) HTML (1 KB)  PDF (591 KB)  ( 103 )
118 Analgesic effect of Dexmedetomidine combined with Tramadol intraperitoneal injection on bone cancer pain rats
YUAN Xiaomin DONG Xiang▲
Objective To study analgesic effect of Dexmedetomidine (DEX) combined with Tramadol (TRA) intraperitoneal injection on bone cancer pain rats. Methods One hundred female Wistar rats were randomly divided into 5 groups: T5 group (TRA 5 mg/kg), T10 group (TRA 10 mg/kg), D1 group (DEX 1 μg/kg), T5+D1 group (TRA 5 mg/kg+DEX 1 μg/kg) and NS group (saline of equal volume, 0.5 mL), 20 rats in each group. At 21 days after establishment of bone cancer pain model, 10 rats in each group were injected intraperitoneally with drugs in above groups. The mechanical pain threshold was measured in 10 rats in each group, and the number of spontaneous leg lifting was measured in 10 rats 2 hours before and 2 hours after intraperitoneal administration. Results At 1, 4, 7, 10, 14, 17 and 21 days after model establishment, the mechanical pain threshold of the tibia operation side was lower than that of control side (P < 0.05). There was no significant difference in mechanical pain threshold between D1 group and NS group at different time after administration (P > 0.05). The peak time of analgesia in T5 group and T10 group was 60 minutes after administration, and the threshold of mechanical pain in T10 group was higher than that in T5 group (P < 0.05). The mechanical pain threshold of bone cancer pain rats in T5+D1 group was significantly higher than that in T5 group. Although the peak time of mechanical pain threshold elevation remained unchanged, it was still 60 minutes after administration, but the time of pain threshold elevation was prolonged to 180 minutes after administration (P < 0.05). There was no significant difference in mechanical pain threshold between T10 group and T5+D1 group at different time after administration (P > 0.05). T5 group, T10 group and T5+D1 group had fewer spontaneous leg lifting times 2 hours after administration than 2 hours before administration, and the differences were statistically significant (P < 0.05). At 2 hours after administration, the number of spontaneous leg lifting in T5+D1 group was less than that in T5 group (P < 0.05). Conclusion DEX combined with TRA can reduce the amount of TRA used at the target analgesic level, prolong the analgesic time and reduce the occurrence of adverse reactions at a single dose.
2019 Vol. 16 (28): 118-121 [Abstract] ( 182 ) HTML (1 KB)  PDF (478 KB)  ( 116 )
122 The clinical efficacy of invigorating the kidney, strengthening spleen and eliminating phlegm in the treatment of amnestic mild cognitive impairment
WU Dongyue1 WEI Mingqing2 TIAN Jinzhou2 SHI Jing2
objective To evaluate the efficacy of invigorating the kidney, strengthening spleen and eliminating phlegm in the treatment of amnestic mild cognitive impairment(aMCI). Methods Using a prospective, open and blank-controlled clinical research method, patients admitted to Ward 3, the Department of Neurology of Dongzhimen Hospital of Beijing University of Chinese Medicine and surrounding communities with a cognitive decline function from January 2014 to January 2017 were screened. A total of 122 patients with aMCI were selected according to the diagnosis and inclusion criteria of this study. The subjects were divided into two groups by number table method: the experimental group (105 cases) received the drugs of invigorating the kidney, strengthening spleen and eliminating phlegm, while the control group (17 cases) was not used any medication or therapy to improves cognitive levels. The observation period was 12 weeks, while the mini mental state examination (MMSE) scores and the pattern element scale for dementia (PES-D/11) scores were recorded before and after treatment. Results In terms of overall cognitive assessment, the MMSE score after 12 weeks of treatment in the experimental group was higher than before treatment, and the difference was statistically significant (P ＜ 0.05). The MMSE score in the experimental group was higher than that in the control group, and the difference was statistically significant (P ＜ 0.05). In terms of Chinese medicine syndromes, the score of kidney deficiency, spleen deficiency and phlegm turbid syndrome in experimental group after 12 weeks of treatment were lower than before treatment, and the differences were statistically significant (P ＜ 0.05). The score of kidney deficiency, spleen deficiency and phlegm turbid syndromes in the experimental group were lower than those in the control group, and the differences were statistically significant (P ＜ 0.05). There were 5 cases (6.4%) of adverse reactions in experimental group, and the patients were relieved spontaneously for 1 week without serious adverse events. Conclusion The curative effect and safety of invigorating the kidney, strengthening spleen and eliminating phlegm for the treatment of aMCI for 12 weeks is good. The long-term curative effect can be verified by large-sample and long-course clinical trials.
2019 Vol. 16 (28): 122-125 [Abstract] ( 184 ) HTML (1 KB)  PDF (762 KB)  ( 83 )
126 Based on intestinal alkaline phosphatase signaling pathway to discuss the mechanism of acupuncture maintaining intestinal flora-immune stability
MAO Wenjiao1 ZHANG Hongmei2
The alteration of intestinal microflora is considered to be the cause of the pathogenesis of Irritable bowel syndrome (IBS). Intestinal flora-immune disorders lead to changes in the intestinal microenvironment, which induce low-grade inflammation of the intestines. Intestinal alkaline phosphatase (IAP) considered as a potential regulator of intestinal microflora, protects the host from intestinal inflammation and tissue damage mainly in the colon, while is an important mucosal defense factor necessary to maintain the stability of the intestinal environment. Based on the above poinits, IAP-related pathway may be a new entry point to elucidate the molecular biological mechanism of acupuncture on intestinal flora-immune stability, to promote the understanding of the relationship between treatment from spleen and regulation of intestinal flora, and to enrich the scientific connotation of treatment from spleen in Chinese medicine.
2019 Vol. 16 (28): 126-129 [Abstract] ( 210 ) HTML (1 KB)  PDF (767 KB)  ( 265 )
130 Discussion on the mechanism and acupuncture treatment of vertigo in Lingshu
WU Min1 LIANG Jiaqi2 LIU Longtao3▲
Lingshu is an important part of Huangdi Neijing, in which, the etiology, pathogenesis and treatment of vertigo are discussed in detail. Mentioned etiology of vertigo includes heart and kidney attacked by pathogenic qi, marrow sea insufficiency, abnormal circulation of ying (nutrient qi) and wei (guardian qi), and lack of qi in upper part of body. The principles of treatment to vertigo include adjusting the qi of organs, regulating deficiency and excess of channels and collaterals, and guiding the ying qi and wei qi. Lingshu emphasizes the important role of Taiyang Bladder Meridian of Foot in the treatment of vertigo. In acupuncture treatment, the acupoints of Taiyang Bladder Meridian of Foot are selected more often. The treatise about vertigo introduced in Lingshu is of great value to the diagnosis and treatment of vertigo in Chinese medicine.
2019 Vol. 16 (28): 130-132,137 [Abstract] ( 205 ) HTML (1 KB)  PDF (747 KB)  ( 116 )
133 Review on the etiology, pathogenesis and the thought of typing treatment of functional dyspepsia based on syndrome differentiation theory
FAN Mingming1 BAI Yan1 XUE Yinghua2 WANG Shun1
Based on the theory of treatment based on syndrome differentiation, taking the five-phase theory and the connection of the zang-fu organs and holism as the guidance, this paper analyzes the literatures related to functional dyspepsia in recent 10 years, summarizes the etiology and pathogenesis, syndrome differentiation and therapeutic method, aiming at making up for insufficiency that the symptomatic treatment of Western medicine can not improve symptoms significantly, can not achieve radical effects, and has more adverse reactions. Chinese medicine treatment can effectively relieve the clinical symptoms of patients with functional dyspepsia, and can achieve the goal of radical treatment. The purpose of this paper is to provide reference for related researches and clinical treatment.
2019 Vol. 16 (28): 133-137 [Abstract] ( 161 ) HTML (1 KB)  PDF (846 KB)  ( 112 )
138 Meta-analysis of Tianqi Jiangtang Capsules in the treatment of prediabetes
SUN Lu1 TANG Xianyu1 LIU Na2
Objective To systematically evaluate the effects of Tianqi Jiangtang Capsules on diabetic conversion rate, blood sugar, blood lipid and body mass index in pre-diabetic patients. Methods Computerized Chinese and English databases were retrieved and included in the randomized controlled trial of Tianqi Jiangtang Capsules in the treatment of prediabetes. The retrieval time was 1970-2018 years in December. After literature screening, data extraction, bias risk and evidence quality evaluation, RevMan 5.3 was used for Meta-analysis. Results Included in 7 studies, 1061 patients. Meta-analysis showed that diabetic conversion rate [OR = 0.43, 95%CI (0.32, 0.58), P < 0.000 01], fasting blood sugar [MD = -0.32, 95%CI (-0.49, -0.16), P < 0.0001], postprandial blood sugar [MD = -1.24, 95%CI (-1.86, -0.61), P = 0.0001] in Tianqi Jiangtang Capsules group were lower than those in control group. Conclusion Tianqi Jiangtang Capsules can delay the progression of pre-diabetes to diabetes, but a large number of high-quality studies are needed to confirm it.
2019 Vol. 16 (28): 138-141,146 [Abstract] ( 233 ) HTML (1 KB)  PDF (1641 KB)  ( 90 )
142 Clinical research status and analysis of acupuncture in the treatment of peripheral neuritis
YU Pei1，2 LIU Lu1 LI Zhijuan1 LI Bin1▲
Objective To investigate the present clinical research status of acupuncture in the treatment of peripheral neuritis, and analyze the future research directions. Methods Twenty-one clinical randomized controlled trials (RCTs) of acupuncture treatment for peripheral neuritis from January 2000 to January 2019 were conducted included through the Chinese journal full text database (CNKI), Wanfang database and PubMed database search. The diagnostic criteria, therapeutic criteria, experimental observation period, experimental control design, treatment methods and characteristics of acupoint selection were reviewed and discussed. Results Local acupoint selection was the main treatment for peripheral neuritis by acupuncture. Yangming Meridian of Hand and Foot was the main point selection, which showed the short-term curative effect advantage. However, there was a lack of standardized criteria in diagnostic criteria, therapeutic evaluation, experimental observation cycle and control design. Conclusion Current research shows that acupuncture treatment for peripheral neuritis is effective, but clinical studies are still not enough. Therefore, more precise RCTs are necessary. Meanwhile, adopting more international evaluation standards and objective monitoring indicators to strengthen the evidence and enhance the overall level of research is also required.
2019 Vol. 16 (28): 142-146 [Abstract] ( 162 ) HTML (1 KB)  PDF (827 KB)  ( 82 )
147 Discussion on the related factors influencing the clinical therapeutic effect of infantile massage in children
WANG Wenyi WANG Guimao CAI Junhao JI Qing
Infantile massage is an important part of traditional Chinese medicine. As a special external therapy of traditional Chinese medicine, infantile massage is widely used in the prevention and treatment of children′s diseases because of its unique advantages in the treatment of children′s health care and common clinical diseases in pediatrics. As present, in the course of clinical treatment with infantile massage, sometimes unsatisfactory clinical effects may occur, and even the therapeutic effects of different doctors on the same diseases will be quite different. This article starts with the various factors that influence the clinical therapeutic effect of infantile massage, including intervention time, indications and contraindications, syndrome differentiation, acupoint selection and prescription, manipulation, psychological state of infantile children, physician-patient relationship, daily diet, daily living and so on, and makes an in-depth analysis and elaboration, in order to provide a theoretical basis for doctors engaged in pediatric massage to better grasp the therapy, further improve the clinical efficacy, and open up the thinking of diagnosis and treatment.
2019 Vol. 16 (28): 147-150 [Abstract] ( 163 ) HTML (1 KB)  PDF (719 KB)  ( 53 )
151 Effect of acupuncture cooperate with pricking and cupping therapy on wind-heat type peripheral facial paralysis
LIU Haiyong1 WANG Ran1▲ JIN Guangming2 YAN Jinsheng2
Objective To explore the clinical application effect of acupuncture combined with pricking and cupping in the treatment of wind-heat type peripheral facial paralysis. Methods Two hundred patients with wind-heat type peripheral facial paralysis admitted to the out-patient and in-patient treatment of Sanhe City Hospital and Yanda Hospital in Hebei Province from April 2017 to December 2018 were selected as subjects. According to different treatment methods, they were divided into two groups. One hundred patients in the observation group were treated with acupuncture combined with pricking and cupping, while 100 patients in the control group were treated with conventional western medicine. In both groups, 10 days was one course of treatment, with a total of 2 courses of treatment. The facial nerve function of the patients was evaluated with the score table of the treatment of peripheral facial neuritis (Portmann scale), and the time consumption of the improvement of facial paralysis was recorded. Clinical efficacy, recurrence rate and clinical efficacy satisfaction were compared. Results The treatment time of facial paralysis in the observation group was significantly shorter than that in the control group, while the Portmann score was significantly higher than that in the control group, the differences were statistically significant(all P ＜ 0.05). The recurrence rate of the observation group was lower than that of the control group, and the effective rate was significantly higher than that of the control group, the differences were statistically significant (all P ＜ 0.05). The satisfaction of the observation group was significantly higher than that of the control group, the difference was statistically significant(P ＜ 0.05). Conclusion Acupuncture combined with pricking and cupping to treat wind-heat type peripheral facial paralysis is simple, effective and safe, which can be popularized in clinic.
2019 Vol. 16 (28): 151-153,161 [Abstract] ( 227 ) HTML (1 KB)  PDF (998 KB)  ( 150 )
154 The diagnostic value of marginal zone enhancement features of contrast-enhanced ultrasound in the benign and malignant breast masses identification of BI-RADS 4
CHEN Fei JIA Chunmei LYU Qi WANG Jingjie HU Tian
Objective To explore the diagnostic value of two-dimensional ultrasound (2D-US) and contrast-enhanced ultrasound features in marginal zone for benign and malignant breast masses in BI-RADS 4. Methods From January 2016 to July 2018, 76 patients were collected from the Department of Ultrasound, the First Affiliated Hospital of Shanxi Medical University. A total of 77 breast masses were confirmed by biopsy or surgical pathology. Benign and malignant tumors were divided into two groups. 2D-US and contrast-enhanced ultrasound characteristics of marginal zone in benign and malignant tumors were compared. And the marginal zone and the inside of the masses time-intensity curve (TIC) parameters of the benign and malignant groups were analyzed. Base strength (BI), peak intensity (PI), area under the curve (AUC), peak time (TTP), and gradient (Grad) were observed. They were analyzed for 3 times and the average value was taken. Results Compared with benign group, 2D-US of malignant group showed more irregular edge, spiculate margin, acoustic halo and lobulated edge, the differences were statistically significant (P < 0.05). Contrast-enhanced ultrasound showed radial enhancement, perforator enhancement and circular enhancement, perforation enhancement, unclear boundary, enlarged range after angiography were more common in malignant group, the differences were statistically significant (P < 0.05). In malignant group, BI, PI, AUC and Grad were bigger in marginal zone than the inside of the masses, and TTP was faster than the inside of the mass, the differences were statistically significant (P < 0.05). In benign group, the marginal zone of lesion was not significantly different from the internal mass in the above parameters (P > 0.05). Conclusion There are significant differences between the benign and malignant masses in the 2D-US and contrast-enhanced ultrasound of the marginal zone. The ultrasound signs of the marginal zone of the malignant masses contribute more to the diagnosis of breast cancer than the internal, therefore, focusing on the marginal zone ultrasound characteristics can improve the ability of differential diagnosis between benign and malignant breast mass.
2019 Vol. 16 (28): 154-157 [Abstract] ( 229 ) HTML (1 KB)  PDF (513 KB)  ( 25 )
158 Clinical effect of Cefaclor and Bromhexine Hydrochloride for Suspension in the treatment of acute suppurative tonsillitis in children
XU Minghao LIU Honglin
Objective To observe the clinical efficacy of Cefaclor and Bromhexine Hydrochloride for Suspension in the treatment of acute suppurative tonsillitis in children. Methods One hundred and twenty children with acute suppurative tonsillitis in Kunming Children′s Hospital from June 2017 to June 2018 were randomly divided into the observation group (60 cases) and the control group (60 cases) by random number table method, which were treated with Cefaclor and Bromhexine Hydrochloride for Suspension, Amoxicillin and Clavulanate Potassium Suspension, respectively. Both group continued treatment for 7 days. The clinical efficacy, disappearance time of symptoms and signs, the chamge of tonsil enlargement and white blood cell count, neutrophil and C-reactive protein levels before and after treatment were compared between the two groups. Results The total effective rate of treatment in the observation group was significantly higher than that in the control group, and the difference was statistically significant (P ＜ 0.05). The time of body temperature returning to normal, the disappearance of tonsil purulent secretion and the pharyngeal congestion of the observation group were significantly shorter than those of the control group, and the differences were statistically significant (P ＜ 0.05). After treatment, the tonsil enlargement of the observation group was significantly better than that of the control group, while serum white blood cell count, neutrophil and C-reactive protein levels in the observation were significantly lower than those in the control group, and the differences were statistically significant (P ＜ 0.05). There were no significant differences in the incidence of adverse reactions between the two groups (P ＞ 0.05). Conclusion The treatment of acute suppurative tonsillitis inflammation in children with Cefaclor and Bromhexine Hydrochloride for Suspension is faster and better than Amoxicillin and Clavulanate Potassium Suspension.
2019 Vol. 16 (28): 158-161 [Abstract] ( 272 ) HTML (1 KB)  PDF (397 KB)  ( 108 )
162 Changes and clinical significance of serum Nesfatin-1 level in patients with obstructive sleep apnea syndrome
CAI Wencan LI Tong LIU Yanming QIAN Chunyan CHEN Weijuan
Objective To investigate the expression of serum Nesfatin-1 in patients with obstructive sleep apnea syndrome (OSAS) and its clinical significance. Methods Fifty patients with OSAS admitted to Yuebei People′s Hospital of Shaoguan City, ("our hospital" for short) from June 2016 to June 2017 were selected as OSAS group and divided into mild subgroup (10 cases), moderate subgroup (24 cases) and severe subgroup (16 cases) according to apnea hypopnea index (AHI). Another 30 healthy subjects visited the physical examination center of our hospital in the same period were selected as control group. Serum Nesfatin-1 level, Epworth sleepiness scale (ESS) score, AHI, minimum oxygen saturation (LSaO2) and maximum apnea time (Tmax) were compared between OSAS group and control group. Serum Nesfatin-1 level, ESS score, LSaO2 and Tmax in different subgroups of OSAS were observed. Pearson correlation analysis was used to analyze the correlation between ESS score, AHI, Tmax and LSaO2 and serum Nesfatin-1 level. Subject working characteristic curve (ROC) was used to evaluate the diagnostic efficacy of Nesfatin-1 in OSAS. Results Serum Nesfatin-1 level, ESS score and AHI in OSAS group were significantly higher than those in control group, LSaO2 in OSAS group was significantly lower than that in control group, and Tmax in OSAS group was significantly longer than that in control group (P < 0.05). Serum Nesfatin-1 level, ESS score and Tmax in mild, moderate and severe subgroups increased with the severity of the disease, while LSaO2 decreased with the severity of the disease (P < 0.05). ESS score, AHI and Tmax in OSAS group were positively correlated with serum Nesfatin-1 level (r = 0.711, 0.621, 0.357, all P < 0.05), and LSaO2 was negatively correlated with serum Nesfatin-1 (r = 0.477, P < 0.05). The sensitivity and specificity of serum Nesfatin-1 (≥ 4.8 μg/L) for diagnosis of OSAS were 81.52% and 76.17% respectively. Conclusion The level of serum Nesfatin-1 in OSAS patients is abnormally elevated, and the degree of elevation is related to the severity of the disease, which has guiding significance for clinical diagnosis and treatment.
2019 Vol. 16 (28): 162-165 [Abstract] ( 160 ) HTML (1 KB)  PDF (404 KB)  ( 69 )
166 Application of management accounting in public hospitals under the new situation
ZHENG Deliang1,2,3 WANG Juzhen4
At present, the ministry of finance has issued important documents on management accounting, which greatly promotes the development of enterprise management accounting in China. However, the application of management accounting in public hospitals is relatively slow. With the in-depth development of the market economy and the reform of the medical and health system, the competition in the medical and health market has become increasingly fierce. In this environment, hospitals must recognize the need for management accounting to develop in hospitals in order to survive and achieve sustainable development. Firstly, the article introduces the main contents of the application of management accounting in public hospitals, including the principles of management accounting, hospital decision accounting, hospital control accounting, hospital responsibility accounting and performance evaluation. Secondly, it summarizes the characteristics of the application of management accounting in public hospitals, aiming at improving the efficiency and efficiency of hospital management, focusing on the future development of hospitals and making accounting functions, making accounting function rise to the height of modern comprehensive management, without howing follow the rules and regulations of financial accounting. One more, the significance of applying management accounting to the management of public hospitals is analyzed, including providing the basis for accounting information decision-making needed for the management of hospitals, deepening the performance appraisal of hospitals, and promoting the economic and social double benefits of public hospitals. Finally, this paper puts forward some suggestions on the development and application of management accounting in public hospitals, including recognizing the development situation of hospital management accounting, establishing the management accounting thought of hospital management, strengthening the training of hospital management accounting talents, promoting the informatization construction of hospital management accounting, formulating policies and standardizing the development of management accounting and other measures.
2019 Vol. 16 (28): 166-169,173 [Abstract] ( 192 ) HTML (1 KB)  PDF (519 KB)  ( 52 )
170 Practice and prospects of WeChat small program in clinic appointment registration management system 
WANG Qiuying LI Ang ZHANG Aiying
WeChat small programs take full advantage of WeChat large user volume, in order to simplify the open interface docking of various resources, and WeChat subscription, and WeChat service, parallel, allow a third party independent development scenarios and upload content, for the third party and offer service of mobile portable, is extensive information transmission and the transmission of data connection tool. The application of WeChat small program in outpatient appointment registration management system can provide users with convenient, fast and efficient services, which is conducive to optimizing the medical service process and improving the quality of mobile medical service. Through investigation and research, it is found that there are problems in the application of WeChat small program in outpatient appointment registration management system, such as the number of public medical institutions opened, the quality of registration function is not good, and the connection and cooperation between the information system and tencent medical team is not deep enough. Putting forward the prospect of "small program + public account +APP" to build the mobile medical service ecosystem and "small program + social sharing" to help medical institutions optimize their services.
2019 Vol. 16 (28): 170-173 [Abstract] ( 234 ) HTML (1 KB)  PDF (502 KB)  ( 170 )
174 The practice and thinking of human genetic resources management in drug clinical trial implementation
ZHOU Yan1 REN Yu2 YANG Hongxin1 ZHANG Yong1
This paper describes the Inner Mongolia People′s Hospital drug clinical trial institutions in human genetic resources management to strengthen the system construction, optimize the approval department and process, define the effective management method to be taken when the responsibility of each party is clear. However, there are still some problems in the implementation. In the absence of full-time managers and targeted training, it is necessary to strengthen the traceability management of human genetic sample information and the supervision of third-party testing institutions, improve the auxiliary ability of hospital testing department. It is hoped that the continuous improvement of human genetic resources management will promote the construction of drug clinical trial platform.
2019 Vol. 16 (28): 174-176,185 [Abstract] ( 244 ) HTML (1 KB)  PDF (797 KB)  ( 270 )
177 Effect of Snyder hopes theory on the early rehabilitation in patients with stroke
YOU Jia1 XI Guangjun2
Objective To explore the effect of Snyder hopes theory on the early rehabilitation in patients with stroke. Methods From July 2016 to July 2017, 55 patients with strokes who were treated with early routine rehabilitation interventions in Wuxi People′s Hospital of Jiangsu Province (hereinafter referred to as "our hospital") were used as the control group. From August 2017 to August 2018, 55 stroke patients admitted to our hospital based on Snyder′s hope theory of early rehabilitation intervention were selected as the observation group. The level of hope, daily living ability, neurological deficit degree and exercise compliance were compared before and after intervention. Results After 1 month of intervention, the total score of positive attitude towards the present and future, close relationship with others, positive action and hope level in the observation group were higher than that in the control group (all P ＜ 0.05). The daily living ability scale (ADL) score of the observation group was lower than that of the control group, and the national institutes of health stroke scale (NIHSS) score of the observation group was lower than that of the control group after 1 month of intervention, while the exercise compliance of the observation group was higher than that of the control group, and the differences were statistically significant (all P ＜ 0.05). Conclusion Snyder hopes that the application of early theory in stroke patients can improve their hope level and daily living ability, reduce the degree of neurological deficit, and improve the patient′s exercise compliance, which is worth promoting.
2019 Vol. 16 (28): 177-180 [Abstract] ( 265 ) HTML (1 KB)  PDF (477 KB)  ( 263 )
181 Application effect of nurse standardized training network information platform in ICU new nurses
ZHANG Ran1 WANG Qingzhen2 FENG Yadi1 LI Jing2▲ LIU Yisi3
Objective To explore the effect of network information platform on standardized training of new nurses in ICU. Methods From 2015 August to 2016 August, 50 new nurses recruited in ICU of Beijing Tiantan Hospital Affiliated to Capital Medical University (hereinafter referred to as "our hospital") were selected as the control group, and routine training was adopted. In 2017 August, 46 new nurses recruited in ICU of our hospital were set as intervention group, by training through the network information platform. The clinical nursing ability, theoretical achievement and professional skill achievement of the two groups were compared. Results The self-evaluation of leadership, monitoring of critically ill patients, teaching and cooperation, planning and evaluation, communication, professional development and total score of the intervention group were all higher than those of the control group, with statistically significant differences (all P ＜ 0.05). Peer evaluation of leadership, monitoring of critically ill patients, teaching and cooperation, planning and evaluation, communication, professional development and total score of the intervention group were all higher than those of the control group, with statistically significant differences (all P ＜ 0.05). The theoretical scores and professional skills of the intervention group were higher than those of the control group, with statistically significant differences (all P ＜ 0.05). Conclusion Standardized training of nurses is a crucial link to train qualified nurses. Applying the network information platform to conduct standardized training of new ICU nurses can improve the training effect of new ICU nurses, which is worthy of reference and reference by all hospitals.
2019 Vol. 16 (28): 181-185 [Abstract] ( 162 ) HTML (1 KB)  PDF (520 KB)  ( 37 )
186 Application effect of Orem model in elderly chronic gastritis patients
WANG Sheng JIN Ying LI Huizi
Objective To explore the effect of Orem model in elderly patients with chronic gastritis. Methods The clinical data of 200 elderly patients with chronic gastritis from January 2013 to January 2018 in Dalian Municipal Central Hospital Affiliated Dalian Medical University were analyzed and grouped accord to the order of admission, 90 cases in the conventional mode group and 110 cases in the Orem mode group. The attitude of elderly patients to nursing intervention, the scores of self-care level, quality of life, self-management and self-efficacy, as well as the length of hospital stay before and after the implementation of the model and nursing satisfaction of the two groups were observed. Results The proportion of patients with better attitude towards nursing intervention in Orem model group was higher than that in the conventional model group, with statistically significant difference (P ＜ 0.05). Before the implementation of the model, there was no statistically significant difference in the scores of patients′ self-care level between the two groups (P ＞ 0.05). After the implementation of the model, the scores of self-care level and quality of life of patients in both groups were higher than those before the implementation of the model, the scores of self-care level in Orem model group were higher than those in the conventional model group, with statistically significant differences (all P ＜ 0.05). There was no significant difference in quality of life scores between the two groups (P ＞ 0.05). Before the implementation of the model, there were no statistically significant differences in self-management score and self-efficacy score between the two groups (P ＞ 0.05). After the implementation of the model, the scores of self-management and self-efficacy of patients in both groups were higher than those before the implementation of the model, and the Orem model group was higher than the conventional model group, with statistically significant differences (all P ＜ 0.05). There was no statistically significant difference in the length of hospital stay between the two groups (P ＞ 0.05), while the nursing satisfaction of Orem model group was higher than that of the conventional model group (P ＜ 0.05). Conclusion The application of Orem model in elderly patients with chronic gastritis can improve their self-management level, self-management and self-efficacy, and improve patients′ attitude and satisfaction with nursing intervention, which is worthy of clinical promotion and application.
2019 Vol. 16 (28): 186-封三 [Abstract] ( 159 ) HTML (1 KB)  PDF (637 KB)  ( 41 )
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