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| Based on intestinal alkaline phosphatase signaling pathway to discuss the mechanism of acupuncture maintaining intestinal flora-immune stability |
| MAO Wenjiao1 ZHANG Hongmei2 |
1.Department of Acupuncture, the Second Hospital of Nanjing, Jiangsu Province, Nanjing 210003, China;
2.Department of Clinical Testing Center, the Second Hospital of Nanjing, Jiangsu Province, Nanjing 210003, China |
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Abstract The alteration of intestinal microflora is considered to be the cause of the pathogenesis of Irritable bowel syndrome (IBS). Intestinal flora-immune disorders lead to changes in the intestinal microenvironment, which induce low-grade inflammation of the intestines. Intestinal alkaline phosphatase (IAP) considered as a potential regulator of intestinal microflora, protects the host from intestinal inflammation and tissue damage mainly in the colon, while is an important mucosal defense factor necessary to maintain the stability of the intestinal environment. Based on the above poinits, IAP-related pathway may be a new entry point to elucidate the molecular biological mechanism of acupuncture on intestinal flora-immune stability, to promote the understanding of the relationship between treatment from spleen and regulation of intestinal flora, and to enrich the scientific connotation of treatment from spleen in Chinese medicine.
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[1] Drossman DA. Functional Gastrointestinal Disorders：History，Pathophysiology，Clinical Features and Rome Ⅳ [J]. Gastroenterology，2016，150（6）：1262-1279.
[2] Su T，Liu R，Lee A，et al. Altered Intestinal Microbiota with Increased Abundance of Prevotella Is Associated with High Risk of Diarrhea-Predominant Irritable Bowel Syndrome [J]. Gastroenterol Res Pract，2018，2018：6961783.
[3] Lalles JP. Intestinal alkaline phosphatase：multiple biological roles in maintenance of intestinal homeostasis and modulation by diet [J]. Nutr Rev，2010，68（6）：323-332.
[4] Fawley J，Koehler S，Cabrera S，et al. Intestinal alkaline phosphatase deficiency leads to dysbiosis and bacterial translocation in the newborn intestine [J]. J Surg Res，2017，218：35-42.
[5] Martinez-Moya，P，Ortega-Gonzalez，M，Gonzalez，R，et al. Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats [J]. Pharmacol Res，2012，66（2）：144-153.
[6] Alpers DH，Zhang Y，Ahnen DJ. Synthesis and parallel secretion of rat intestinal alkaline phosphatase and a surfactant-like particle protein [J]. Am J Physiol，1995，268（6 PT 1）：E1205-E1214.
[7] Goldberg RF，Austen WG Jr，Zhang X，et al. Intestinal alkaline phosphatase is a gut mucosal defense factor maintained by enteral nutrition [J]. Proc Natl Acad Sci USA，2008，105（9）：3551-3556.
[8] Yang WH，Heithoff DM，Aziz PV，et al. Recurrent infection progressively disables host protection against intestinal inflammation [J]. Science，2017，358（6370）：eaao5610.
[9] Lalles JP. Intestinal alkaline phosphatase： novel functions and protective effects [J]. Nutr Rev，2014，72（2）：82-94.
[10] Fawley J，Gourlay DM. Intestinal alkaline phosphatase：a summary of its role in clinical disease [J]. J Surg Res，2016，202（1）：225-234.
[11] Goldberg RF，Austen WJ，Zhang X，et al. Intestinal alkaline phosphatase is a gut mucosal defense factor maintained by enteral nutrition [J]. Proc Natl Acad Sci USA，2008，105（9）：3551-3556.
[12] Malo MS，Moaven O，Muhammad N，et al. Intestinal alkaline phosphatase promotes gut bacterial growth by reducing the concentration of luminal nucleotide triphosphates [J]. Am J Physiol Gastrointest Liver Physiol，2014， 306（10）：G826-G838.
[13] Bates JM，Akerlund J，Mittge E，et al. Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota [J]. Cell Host Microbe，2007，2（6）：371-382.
[14] Lalles JP. Luminal ATP：the missing link between intestinal alkaline phosphatase，the gut microbiota，and inflammation? [J]. Am J Physiol Gastrointest Liver Physiol，2014，306（10）：G824-G825.
[15] Alam SN，Yammine H，Moaven O，et al. Intestinal alkaline phosphatase prevents antibiotic-induced susceptibility to enteric pathogens [J]. Ann Surg，2014，259（4）：715-722.
[16] Heneghan AF，Pierre JF，Kudsk KA. JAK-STAT and intestinal mucosal immunology [J]. JAKSTAT，2013，2（4）：e25530.
[17] Deplancke B，Gaskins HR. Microbial modulation of innate defense：goblet cells and the intestinal mucus layer [J]. Am J Clin Nutr，2001，73（6）：1131S-1141S.
[18] Cobo ER，Kissoon-Singh V，Moreau F，et al. Colonic MUC2 mucin regulates the expression and antimicrobial activity of beta-defensin 2 [J]. Mucosal Immunol，2015，8（6）：1360-1372.
[19] Liu W，Hu D，Huo H，et al. Intestinal Alkaline Phosphatase Regulates Tight Junction Protein Levels [J]. J Am Coll Surg，2016，222（6）：1009-1017.
[20] Hamarneh SR，Mohamed MM，Economopoulos KP，et al. A novel approach to maintain gut mucosal integrity using an oral enzyme supplement [J]. Ann Surg，2014，260（4）：706-715.
[21] Fries W，Belvedere A，Vetrano S. Sealing the broken barrier in IBD：intestinal permeability，epithelial cells and junctions [J]. Curr Drug Targets，2013，14（12）：1460-1470.
[22] Wang D，Zhou X，She R，et al. Impaired intestinal mucosal immunity in specific-pathogen-free chickens after infection with very virulent infectious bursal disease virus [J]. Poult Sci，2009，88（8）：1623-1628.
[23] Chen Z，Xie J，Wang B，et al. Effect of gamma-aminobutyric acid on digestive enzymes，absorption function，and immune function of intestinal mucosa in heat-stressed chicken [J]. Poult Sci，2014，93（10）：2490-2500.
[24] Yang Y，Wandler AM，Postlethwait JH，et al. Dynamic Evolution of the LPS-Detoxifying Enzyme Intestinal Alkaline Phosphatase in Zebrafish and Other Vertebrates [J]. Front Immunol，2012，3：314.
[25] Park BS，Song DH，Kim HM，et al. The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex [J]. Nature，2009，458（7242）：1191-1195.
[26] Yamamoto M，Akira S. Lipid A receptor TLR4-mediated signaling pathways [J]. Adv Exp Med Biol，2010，667：59-68.
[27] Huhta H，Helminen O，Kauppila JH，et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer [J]. J Histochem Cytochem，2016，64（8）：470-482.
[28] Fawley J，Gourlay DM. Intestinal alkaline phosphatase：a summary of its role in clinical disease [J]. J Surg Res，2016，202（1）：225-234.
[29] Dolowschiak T，Mueller AA，Pisan LJ，et al. IFN-gamma Hinders Recovery from Mucosal Inflammation during Antibiotic Therapy for Salmonella Gut Infection [J]. Cell Host Microbe，2016，20（2）：238-249.
[30] Estaki M，Decoffe D，Gibson DL. Interplay between intestinal alkaline phosphatase，diet，gut microbes and immunity [J]. World J Gastroenterol，2014，20（42）：15650-15656.
[31] Gunnarsson J，Simren M. Peripheral factors in the pathophysiology of irritable bowel syndrome [J]. Dig Liver Dis，2009，41（11）：788-793.
[32] 徐万里.基于肠道菌群-SCFAs-宿主互动理论探讨“调神健脾”针法对IBS-D患者内脏高敏感调节机制[D].南京：南京中医药大学，2016.
[33] 李丹丹.基于TLR2/4-MYD88-NF-κb信号通路研究调神健脾针刺法治疗腹泻型肠易激综合征的机制[D].南京：南京中医药大学，2018.
[34] 梁世杰.调神健脾针刺法对腹泻型肠易激综合征患者下丘脑-垂体-肾上腺轴的影响[D].南京：南京中医药大学，2017. |
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