|
|
|
| Electron staining of myocardial amyloidosis by different preparation methods and tea instead of uranium |
| ZHAO Ranxu1 WEI Yingjie1▲ LI Li2 WANG Qingzhi2 LU Yang2 XU Hongyu2 YOU Yiwei2 |
1.Department of Electron Microscopy Platform of National Key Laboratory of Cardiovascular Diseases, Chinese Academy of Medical Sciences Peking Union Medical College National Center for Cardiovascular Diseases Fuwai Hospital, Beijing 100037, China;
2.Department of Pathology, Chinese Academy of Medical Sciences Peking Union Medical College National Center for Cardiovascular Diseases Fuwai Hospital, Beijing 100037, China |
|
|
|
Abstract Objective To test whether oolong tea extract can be used as an electronic stain instead of uranyl acetate (UA) for myocardial amyloidosis staining. In the process of staining, different staining methods were used and improve the preparation methods and compare their safety and efficiency. Methods A total of 40 cases of clinically suspected amyloidosis were collected from Fuwai Hospital of the Chinese Academy of Medical Sciences from January to December 2017, and 10 cases of cardiac tissue positive after pathological observation and special staining. The myocardial tissues were prepared by conventional encapsulation and improvement of resin 812, and then two kinds of different stains (3% uranyl acetate solution, 0.5% oolong tea extract) were applied to the ultrathin section samples under the conditions of counterstaining lead or not counterstaining lead, and two different staining methods (drip method, slice box staining) electronic staining, and then the differences were compared under a transmission electron microscope. Results Under the condition of repeated staining of lead, the results of staining of amyloid substance in myocardium with two kinds of electron staining agents and the improved preparation method were all satisfactory. The background of the slice was clean, the contrast was high and the obtained image was clear. Conclusion In the observation of myocardial amyloidosis, oolong tea extract with section box staining and the improved preparation method can more safely and quickly replace dioxide-uranium acetate for transmission electron microscope electron staining.
|
|
|
|
|
|
[1] 孙洋，赵红.电镜在心脏疾病中的研究及诊断意义[J].临床与病理杂志，2017，37（7）：1524-1528.
[2] 李莉，段雪晶，孙洋，等.免疫组织化学指标在心脏淀粉样变性分型中的应用价值[J].中华病理学杂志，2018，47（2）：105-109.
[3] 赵娟，张彦妹，郑乃智.β-淀粉样蛋白增加Wistar大鼠癫痫发作易感性的行为学研究[J].中国医药导报，2017， 14（27）：25-25，36.
[4] 于洋，王鸿，田俊萍，等.心率变异性在冠心病中的应用价值[J].中国医药导报，2017，14（11）：56-59.
[5] HE Xiao-Hua，Bin L. LR-White resin and tea：a quick and safe bacterial cells preparation Method for transmission electron microscopy [J]. Microbiology China，2015，42（10）：2041-2047.
[6] Watson ML. Staining of tissue sections for electron microscopy with heavy metals [J]. Journal of Biophysical and Biochemical Cytology，1958，4（6）：727-730.
[7] Kajikawa K，Yamaguchi T，Katsuda S，et al. An improved electron stain for elastic fibers using tannic acid [J]. J Electron Microsc（Tokyo），1975，24（4）：287-289.
[8] Tanaka K，Nakamura H. A comparison of four electron staining methods for elastic fibers [J]. Journal of Saitama Medical School，1986，13：219-226.
[9] Sato S，Sasaki Y，Adachi A，et al. Use of oolong tea extract（OTE）for elastin staining and enhancement in ultrathin sections [J]. Med Electron Microsc，2003，36（3）：179-182.
[10] Carpentier A，Abreu S，Trichet M，et al. Microwaves and tea：new tools to process plant tissue for transmission electron microscopy [J]. J Electron Microsc（Tokyo），2012， 247（1）：94-105.
[11] Nakakoshi M，Nishioka H，Katayama E. New versatile staining reagents for biological transmission electron microscopy that substitute for uranyl acetate [J]. J Electron Microsc（Tokyo），2011，60（6）：401-407.
[12] Miller AA，Simakova AV. Application of method of OTE staining of ultrathin sections based on example of microsporidia（Protozoa：Microsporidia）[J]. Cell and Tissue Biology，2010，4（1）：109-115.
[13] 宋西娇，陈浙，何步远，等.西瓜花叶病毒和小西葫芦黄花叶病毒复合侵染南瓜的透射电镜诊断[J].电子显微学报，2015，34（2）：126-131.
[14] 张旭，程明，徐进，等.透射电镜在胸膜恶性肿瘤诊断中的作用[J].中国体视学与图像分析，2015，20（1）：74-84.
[15] 黄远洁，莫肖敏，孟春梅，等.3种常规透射电子显微镜超薄切片染色方法的对比分析[J].广西医科大学学报，2015，32（6）：912-913.
[16] Yamaguchi K，Suzuki K，Tanaka K. Examination of electron stains as a substitute for uranyl acetate for the ultrathin sections of bacterial cells [J]. J Electron Microsc（Tokyo），2010，59（2）：113-118.
[17] Sato S，Adachi A，Sasaki Y，et al. Oolong tea extract as a substitute for uranyl acetate in staining of ultrathin sections [J]. J Microsc，2008，229（1）：17-20.
[18] Miller AA，Simakova AV. Use of the OTE-staining method for ultrathin sections on the example of microsporidia（Protozoa:Microsporidia）[J]. Tsitologiia，2009，51（9）：741-747.
[19] Sato S，Kishikawa T. Ultrastructural study of the alveolar lining and the bronchial mucus layer by block staining with oolong tea extract：the role of various surfactant materials [J]. Med Electron Microsc，2001，34（2）：142-151.
[20] Stempak JG，Ward RT. An improved staining method for electron microscopy [J]. J Cell Biol，1964，22（3）：697-701 |
|
|
|
