miR-361-3p调节恶性胶质瘤细胞功能的研究

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Abstract Objective To study the role of miR-361-3p in glioblastoma (GB) cells. Methods LN299, U87MG and normal human astrocytes (NHA) were cultured. miR-361-3p mimic or inhibitor and MTCP1 siRNA (si-MTCP1) were transfected into U87MG cells, respectively. Gene expression was tested by RT-PCR and Western blot. Cell proliferation and apoptosis were measured by CCK-8 and Caspase3 kits, respectively. The binding of miR-361-3p and MTCP1 were detected by luciferase reporter assay. Results Relative to NHA, miR-361-3p levels were lower in LN299 and U87MG. Transfected with mimic, MTCP1 levels and the proliferation of U87MG cells were decreased, and the Caspase3 activity was increased, and luciferase activity in WT-MTCP1 was decreased. Furthermore, transfected with inhibitor, the proliferation of U87MG cells were increased, while the apoptosis rate in the si-MTCP1 group was increased. Conclusion MiR-361-3p may inhibit the proliferation and promote apoptosis of GB cells by inhibiting MTCP1.

Key words： Glioblastoma Cell proliferation Apoptosis miR-361-3p

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	GUO Xiaoye MAO Ping WANG Jia LIAN Haiping WANG Wei BAI Xiaobin SONG Jinning

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GUO Xiaoye MAO Ping WANG Jia LIAN Haiping WANG Wei BAI Xiaobin SONG Jinning. Studies of miR-361-3p in regulating the function of glioblastoma cells[J]. 中国医药导报, 2019, 16(28): 7-11.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I28/7

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