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| Analgesic effect of Dexmedetomidine combined with Tramadol intraperitoneal injection on bone cancer pain rats |
| YUAN Xiaomin DONG Xiang▲ |
| Department of Anesthesiology, the Ninth People′s Hospital Affiliated to Medical College of Shanghai Jiaotong University, Shanghai 200011, China |
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Abstract Objective To study analgesic effect of Dexmedetomidine (DEX) combined with Tramadol (TRA) intraperitoneal injection on bone cancer pain rats. Methods One hundred female Wistar rats were randomly divided into 5 groups: T5 group (TRA 5 mg/kg), T10 group (TRA 10 mg/kg), D1 group (DEX 1 μg/kg), T5+D1 group (TRA 5 mg/kg+DEX 1 μg/kg) and NS group (saline of equal volume, 0.5 mL), 20 rats in each group. At 21 days after establishment of bone cancer pain model, 10 rats in each group were injected intraperitoneally with drugs in above groups. The mechanical pain threshold was measured in 10 rats in each group, and the number of spontaneous leg lifting was measured in 10 rats 2 hours before and 2 hours after intraperitoneal administration. Results At 1, 4, 7, 10, 14, 17 and 21 days after model establishment, the mechanical pain threshold of the tibia operation side was lower than that of control side (P < 0.05). There was no significant difference in mechanical pain threshold between D1 group and NS group at different time after administration (P > 0.05). The peak time of analgesia in T5 group and T10 group was 60 minutes after administration, and the threshold of mechanical pain in T10 group was higher than that in T5 group (P < 0.05). The mechanical pain threshold of bone cancer pain rats in T5+D1 group was significantly higher than that in T5 group. Although the peak time of mechanical pain threshold elevation remained unchanged, it was still 60 minutes after administration, but the time of pain threshold elevation was prolonged to 180 minutes after administration (P < 0.05). There was no significant difference in mechanical pain threshold between T10 group and T5+D1 group at different time after administration (P > 0.05). T5 group, T10 group and T5+D1 group had fewer spontaneous leg lifting times 2 hours after administration than 2 hours before administration, and the differences were statistically significant (P < 0.05). At 2 hours after administration, the number of spontaneous leg lifting in T5+D1 group was less than that in T5 group (P < 0.05). Conclusion DEX combined with TRA can reduce the amount of TRA used at the target analgesic level, prolong the analgesic time and reduce the occurrence of adverse reactions at a single dose.
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