EZH2在大鼠睾丸扭转后缺血再灌注损伤中的作用及机制

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Abstract Objective To investigate the effects of EZH2 on testicular ischemia-reperfusion injury in rats. Methods Eighteen adult healthy male SD rats were randomly divided into control group, ischemia group and EZH2 inhibition group, 6 rats in each group. The free testicles were immediately repositioned and fixed after clockwise torsion of 720° in control group. The free testicles were rotated torsion of 720° and maintained for 2 h in ischemia group and EZH2 inhibition group. Before clockwising torsion, EZH2 inhibitor group was intraperitoneally injected with E2H2 inhibitor UNC1999 [50 mg/(kg·d)] for 3 days, while the control group and ischemia group were intraperitoneally injected with the phosphate buffer saline (PBS) of same volume. Rats in each group were sacrificed after 4 h of torsion and reposition, and the ischemic testicular tissues were obtained. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. Histopathoiogical changes and germ cell apoptosis indices (AI) were observed using hematoxylin-eosin staining and in situ terminal transferase labeling technique. The expressions of EZH2, NF-E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were detected using Western blot. Results Compared with control group, the activity of SOD and the expression of Nrf2 and HO-1 were decreased after reduction, the expressions of MD, AI and EZH2 were increased in ischemic group and EZH2 inhibitor group, the differences were statistically significant (all P < 0.05). Compared with ischemic group, the activity of SOD and the expression of Nrf2 and HO-1 were increased after reduction, the expressions of MDA, AI and EZH2 were decreased in EZH2 inhibitor group, the differences were statistically significant (all P < 0.05). Conclusion EZH2 plays an important role in testicular ischemia-reperfusion injury. EZH2 inhibitor exerts a beneficial effect of testicles torsion repositioned after ischemia-reperfusion injury. This effect may be achieved through enhancing Nrf2/HO-1 expression and inhibiting apoptosis.

Key words： Zeste homologous enhancer 2 Testicle Torsion Ischemia

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	ZHONG Yunping ZHANG Jianjian WANG Lei LIU Xiuheng▲

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ZHONG Yunping ZHANG Jianjian WANG Lei LIU Xiuheng▲. Effect of EZH2 on testicular ischemia-reperfusion injury in rats[J]. 中国医药导报, 2019, 16(28): 21-24.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I28/21

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