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2017 Vol. 14, No. 21
Published: 2017-07-25
 
 
4 Preparation of hyaluronic acid modified CS-g-PEI/pDNA nanoparticles and mediated gene transfection against chondrocytes in vitro
CHEN Mingwei1 FAN Binbin1 LU Huading2
Objective To investigate the feasibility of hyaluronic acid modified CS-g-PEI/pDNA nanoparticles and mediated gene transfection against osteoarthritis. Methods CP was engaged with pEGFP into nanopartricles by using complex coaceration method and then covering the CP/pDNA with HA-SH, HA-SH forming disulfide cross-linked gene complex, and on the surface of the CP/pDNA form cross-linked network with HA, synthesis HA-CP/pDNA; the morphy of nanoparticle was obtained by TEM, the particle size and surface potential were measured by Malvern particle size analyzer; The pDNA binding ability with CP and the influence of HA-SH on CP were evaluated by gel retardation assay; the cytotoxicity of HA-CP/pDNA nanoparticles was evaluated by CCK-8 assays; HA-CP/pDNA nanoparticles, nake pDNA, CS/pDNA, CP/pDNA nanoparticles and lipofectamineTM2000 transfected chondrocytes, the transfection efficiency was measured by flurescence microscope, flow cytometry. Results ①TEM showed that the nanoparticles were well-formed spherical shapes with compact structure, and increased with HA-SH∶CP weight ratio increasing, nanoparticle sizes decreased firstly then increased, the surface potentials gradually decrease. ②The cytotoxicity of HA-CP/pDNA nanoparticles had lower cellular toxicity than LipofectamineTM2000 (P < 0.05). ③The HA-CP/pDNA nanoparticles transfection efficiency for chondrocytes was highly improved compared to CS/pDNA, CP/pDNA nanoparticles (P < 0.05). ④Free HA decrease HA-CP/pDNA nanoparticles transfection efficiency notably. Conclusion HA-CP/pDNA has lower cytotoxicity and higher transfection efficiency, and it has the ability of targeting chondrocytes.
2017 Vol. 14 (21): 4-9 [Abstract] ( 173 ) HTML (1 KB)  PDF (1162 KB)  ( 135 )
10 Research on the effect of Tripterygium wilfordii attenuated toxicity by compatibility
HE Fenglei1 CHEN Guiying2 PENG Fuquan1 CHEN Yanqin2 GUO Qiuping2 MO Guoqiang1 GUO Zhonghui1
Objective To observe the toxicity effect of Tripterygium wilfordii and the influence on the toxicity after compatibility with Chinese nourishing kidney herbs. Methods Sixty SD rats were randomly divided into negative control group, Tripterygium wilfordii group and compatibility group (Tripterygium wilfordii combined with Cuscuta chinensis, Fructus Lycii and Epimedium davidii) according to the body weight of animals, with 20 rats in each group. All groups were administrated with corresponding drugs by intragastric administration once a day for 30 days, the rats of negative control group were treated with 0.5% sodium carboxyl methyl cellulose (CMC-Na). During the test, the physical signs, behaviors, gland secretion, respiratory and stool characters of animals were observed every day, and weight was measured every week. After 30 days, the animals were killed for the examination of hematology, hormone levels and pathology. Results Compared with the negative control group, the body weight of Tripterygium wilfordii group was grown slowly (P ＜ 0.05), the hematological indexes such as white blood cell count (WBC), hemoglobin (HGB), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), reticulocyte count (RET) and RET% were decreased significantly (P ＜ 0.05), neutrophile granulocyte (NEUT), NEUT% and the ratio of lymphocyte (LYMPH%) were decreased significantly (P ＜ 0.05); hormone examination showed that follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (TTE) were decreased (P ＜ 0.05) in the Tripterygium wilfordii group; histopathologic examination showed that the male ductus epididymis was empty, the lumen was narrow, and no sperm or cell components in the lumen were found in the Tripterygium wilfordii group. Compared with Tripterygium wilfordii group, the body weight of compatibility group showed no significant difference, the hematology indexes such as WBC, HGB and RET were increased (P ＜ 0.05), TTE level was increased (P ＜ 0.05) and the degree of epididymal lesions was reduced in compatibility group. Conclusion Under the experimental conditions, Tripterygium wilfordii has obvious toxic effects on reproductive system and blood system. Compatibility of Cuscuta chinensis, Fructus Lycii and Epimedium davidii can reduce the toxicity of Tripterygium wilfordii on reproductive system and blood system, which has obvious effects of attenuated toxicity by compatibility.
2017 Vol. 14 (21): 10-13 [Abstract] ( 168 ) HTML (1 KB)  PDF (802 KB)  ( 165 )
14 Study on the expression levels of KA2 in kainic acid-induced epileptic rat
WANG Jing YIN Liang LYU Yongtao FENG Xiaoya
Objective To determine the expression levels of multifunction cytokine AK2 in kainic acid-induced epileptic rat, and to investigate whether AK2 participates in the pathogenesis of EP. Methods 60 male SD rats were divided into control group (10 cases) and model group (50 cases), the animal model of temporal lobe epilepsy was established in model group by intra cerebroventricular injection of KA. Then the rats in the model group were divided into 6 hours group, 12 hours group, 1 day group, 3 days group and 1 week group, with 10 cases in each group, according to the different time points after seizures. AK2 mRNA and protein expression in frontal lobe and hippocampus tissue were measured by semi-quantitative RT-PCR and Western blotting. Results In the model group, expression levels of AK2 in both front al lobe and hippocampus tissue were significant increased compared with the control group (P < 0.05). And the expression levels were reached the highest 3 days after seizures and then gradually declined 1 week later (P < 0.05). Conclusion AK2 may participate in the pathophysiological process of EP and provide a new mechanistic basis for the pathogenesis of epilepsy.
2017 Vol. 14 (21): 14-17 [Abstract] ( 167 ) HTML (1 KB)  PDF (721 KB)  ( 89 )
18 Study on the acute toxicity of Prunus mira oil in rats and rabbits
SUN Weijun1* ZHOU Xi2* WANG Zhang3 ZHANG Jing3 GAN Dali1 LIANG Yuan1 FAN Gang3 YIN Hongxiang3
Objective To study the acute toxicity of Prunus mira oil used in mice, rats and rabbits. Methods 40 KM mice which were half male and half female were divided into blank control group and Prumus mira oil group, each group had 20 mice. Within 24 hours, the mice were given normal saline and Prumus mira oil with one maximum dosage amounting to 289.224 g dried medicinal herbs/(kg·d) in a total of 3 times through mouth. 40 SD rats which were half male and half female were divided into blank control group and Prumus mira oil group, each group had 20 mice. Within 24 hours, the rats were also given normal saline and Prumus mira oil with one maximum dosage of 144.612 g dried medicinal herbs/(kg·d) in a total of 3 times in the same way. 16 New Zealand rabbits which were half male and half female were divided into blank control group and Prumus mira oil group, each group had 8 rabbits. Within 24 hours, the rabbits were given normal saline and Prumus mira oil with one maximum amount of 482.28 mg dried medicinal herbs/(cm2·d) 3 times through skin. And the toxic effects (death, poisoning symptoms) and the severity and recovery, as well as delayed reaction shown on these three animals were carefully observed within 14 days. Results There was no death, poisoning symptoms and organ abnormality visible to the naked eye. Compared with the blank control group, the mice and rats produce loose stool and oily hair after being given medicine within 1-3 d, but return to normal in 4 d. Compared with the blank control group, weights were significantly increased in the male mice (P < 0.01), but significantly decreased in the male rats (P < 0.01) after taking Prunus mira oil of 7 d (P < 0.01), and the weights of female rabbits decreased significantly after taking Prunus mira oil of 2 d through skin (P < 0.05). Compared with the blank control group, the feed consumption of mice in the Prumus mira oil group were decreased after 1 d medication (P < 0.01). Compared with the blank control group, the average hemoglobin concentration of mice in the Prumus mira oil group were abnormal (P < 0.05), but their blood biochemical indexes showed no obvious change. Some blood indexes [RDW-CV, PLT, PCT (P < 0.05) as well as Mid% (P < 0.01)] and blood biochemical indexes [ALB, TP, GLO, CHOL, BUN, T-Bil and TG (P < 0.05) as well as CREA (P < 0.01)] showed abnormalities in rats of the Prumus mira oil group. Certain blood indexes [PLT, GR#, PCT, Mid%, PDW, LY% and GR% (P < 0.05)] and blood biochemical indexes [AST and CREA (P < 0.05)] showed abnormalities in rabbits of the Prumus mira oil group. Conclusion Maximum dosage given to the mice and rats through mouth are respectively 289.224 g dried medicinal herbs/(kg·d) and 144.612 g dried medicinal herbs/(kg·d), while the maximum dosage given to the rabbits through skin is 482.28 mg dried medicinal herbs/(cm2·d). There is no security problem, but the blood routine and blood biochemistry indexes should be monitored during clinical application.
2017 Vol. 14 (21): 18-24 [Abstract] ( 164 ) HTML (1 KB)  PDF (699 KB)  ( 71 )
25 Analysis on the effectiveness and safety of stellate ganglion block in the treatment of migraine and its influence on the hemodynamics of intracranial and cervical arteries
WANG Huiying1,2 LI Hongliang3 WANG Baoguo1
Objective To investigate the effectiveness and safety of stellate ganglion block (SGB) in the treatment of migraine and its influence on the hemodynamics of intracranial and cervical arteries. Methods Eighty patients met with the diagnostic criteria of migraine treated in Department of Pain, Sanbo Brain Hospital, Capital Medical University from June 2014 to January 2015 were selected. The pain degree was evaluated by numerical rating scale (NRS), the postoperative complications were recorded, and the efficacy was evaluated after operation for 6 months. The bilateral cervical arteries and intracranial arteries were detected by transcranial doppler sonography to record the indexes of hemodynamics. Results Compared with those before SGB, the scores of NRS after SGB were decreased, the difference was statistically significant (P ＜ 0.05). The total effective rate of SGB after 6 months was 91.25%. Compared with that before block and the opposite block side, SGB could significantly increase the diastolic flow velocities of the block side of external carotid artery and common carotid artery, decrease the pulse index, the differences were all statistically significant (P ＜ 0.05). The blood flow velocities, the changes of flow velocities and the pulse indexes of the block side and the opposite block side of the other cervical arteries and intracranial arteries had no statistically significant differences before and after SGB (P ＞ 0.05). After SGB, among 80 cases of patients, there were 6 cases (7.50%) with hoarseness (completely disappeared within 1 h), 2 cases (2.50%) with transient upper limb numbness, 3 cases (3.75%) with brachial plexus block, 4 cases (5.00%) with puncture site pain, 3 cases (3.75%) with foreign body sensation. Conclusion SGB is efficacious and safe to treat migraine. SGB can relieve the symptoms of migraine may be achieved by lowering the vascular resistance of external carotid artery and common carotid artery, speeding up their blood flow velocity, and mildly lowering the vascular resistance of intracranial arteries.
2017 Vol. 14 (21): 25-28,49 [Abstract] ( 244 ) HTML (1 KB)  PDF (1077 KB)  ( 111 )
29 Butein exerts pro-apoptotic activity in lung adenocarcinoma A549 cells via the regulation of STAT3 signaling
CHEN Shaoyang YUE Honglin LIU Xu
Objective To investigate the pro-apoptotic effect of Butein (BTN) in lung adenocarcinoma A549 cells and the role of STAT3 signaling in this process. Methods A549 cells were cultured and treated with different concentrations of BTN (0, 25, 50 μmol/L) for 24 h. BTN 0 μmol/L was taken as the control group. The survival rate, apoptotic rate, cell migration, ROS generation, Caspase-3 activity were respectively detected after treatment. Western blot was used to detect expression of p-STAT3, STAT3, Bcl-2 and Bax. NAC 5 mmol/L was used to block the generation of ROS, then BTN treatment (0, 50 μmol/L) was implemented for further detection as mentioned. Besides, in vivo studies were also used to verify whether abdominal injection with BTN (2, 4 mg/kg) exerted anticancer activity, while the control group was injected with PBS. Results Compared with the control group, BTN (25, 50 μmol/L) treatment resulted in reduction of cell viability and migration (P < 0.05), promotion of cell apoptosis (P < 0.05), up-regulation of ROS and Caspase-3 levels (P < 0.05). BTN treatment resulted in a significant down-regulation of p-STAT3 and Bcl-2, and up-regulation of Bax (P < 0.05). Moreover, inhibition of ROS by NAC antagonized the anti-STAT3 phosphorylated and pro-apoptotic role of BTN on A549 cells (P < 0.05). In vivo studies showed that compared the control group, tumor volume and p-STAT3 expression in nude mice were significantly reduced after BTN treatment (P < 0.05). Conclusion BTN treatment effectively inhibits cell viability and promotes apoptosis in A549 cells, which may be related to the up-regulation of ROS level thus inhibiting STAT3 phosphorylation.
2017 Vol. 14 (21): 29-33 [Abstract] ( 172 ) HTML (1 KB)  PDF (1296 KB)  ( 32 )
34 The progress of the effect of immune-endocrine modulation at the maternal-fetal interface on early pregnancy
JIANG Guojing SHEN Mingjie ZENG Weiwei QI Cong
Pregnancy is an example of the natural allogeneic transplantation. The success of pregnancy depends mostly on a successful immune-endocrine cross-modulation at the maternal–fetal interface. Hormones have important effect on early pregnancy including progesterone, estradiol and human chorionic gonadotrop in play essential roles in the immune crosstalk at the maternal-fetal interface. This review focuses on the immunomodulatory effect of these hormones during early pregnancy on the establishment of maternal-fetus immune tolerance, invasion of trophoblastic cells and formation of placenta. The present review will provide important insights into novel therapeutic strategies of the recurrent spontaneous miscarriage, pre-eclampsia and intrauterine growth restriction.
2017 Vol. 14 (21): 34-37,56 [Abstract] ( 208 ) HTML (1 KB)  PDF (635 KB)  ( 91 )
38 Research progress of microRNA and lncRNA in myocardial fibrosis
GAO Yuqiu JIAO Lei ZHANG Ying
Myocardial fibrosis is the process of excessive accumulation of collagen fibers in myocardial tissue. In recent years, the role of non coding RNA including small RNA (miRNA) and long non-coding RNA (lncRNA), has become a hot topic. miRNA and lncRNA regulate the occurrence and development of myocardial fibrosis by activating multiple signaling pathways and mechanisms. This article reviews the mechanism and research progress of miRNA and lncRNA in myocardial fibrosis.
2017 Vol. 14 (21): 38-41 [Abstract] ( 225 ) HTML (1 KB)  PDF (641 KB)  ( 59 )
42 Roles of nursing staff in treatment of STEMI under operating mode of chest paincenter
HOU Jixin LUO Jing HUANG Xiuping ZHOU Peng WANG Peijian
With chest pain as the main clinical manifestation, acute myocardial infarction, aortic dissection and pulmonary embolism have the characteristics of short time window for treatment, quick change and high mortality, they are serious harm to human health and life. The chest pain center can provide a rapid diagnosis and treatment for the patients with severe chest pain as the main clinical manifestations, especially in patients with ST segment elevation acute myocardial infarction (STEMI). Therefore, the operation of chest pain center has great significance to improve the treatment of acute chest pain, especially in patients with STEMI. In order to make high risk chest pain patients, especially STEMI patients get timely treatment, the construction and operation of chest pain centers need to cooperate actively with different subjects, including the cooperation of nursing team. However, there is still a lack of standardized and unified treatment nursing process of chest pain at present. In addition, informations about the roles of nursing staff in the construction and operation of chest pain center is also less. In this paper, it reviewes the roles of the nursing team in the treatment of STEMI patients in chest pain center, in order to provide a reference for the peer.
2017 Vol. 14 (21): 42-45 [Abstract] ( 181 ) HTML (1 KB)  PDF (606 KB)  ( 136 )
46 Research progress on nursing care of infants with hydrocephalus treated by hyperbaric oxygen
LI Lin ZHANG Yu XIAO Hong GUO Dazhi ZHANG Dunxiao CAO Jianqiu WANG Haidong
This paper reviews the nursing progress of hyperbaric oxygen therapy for infants with hydrocephalus, introduces routine nursing operation mode, nursing intervention mode, holistic nursing model and comprehensive nursing model in hyperbaric oxygen therapy for infants with hydrocephalus, analyzes the application limitations of various nursing models. This paper finds that the comprehensive nursing is a kind of high quality nursing mode, in order to provide reference for improvement of specialized nursing level of hyperbaric oxygen treatment.
2017 Vol. 14 (21): 46-49 [Abstract] ( 154 ) HTML (1 KB)  PDF (606 KB)  ( 56 )
50 Research progress of MTHFR C677T polymorphism and plasma homocysteine level with coronary heart diseases patients with heart failure
ZHANG Jianwu1 CHEN Liang2
There are many kinds of mutation sites in methylenetetrahydrofolate reductase (MTHFR) gene, among which, C677T is the most common one. MTHFR gene mutation causes the decrease of enzyme activity and heat resistance, causes hyperhomocysteinemia. Homocysteine (Hcy) is an independent risk factor for coronary heart diseases. The average level of Hcy is higher in patients with heart failure. Hcy level increases gradually as the cardiac function deteriorates. This paper reviews the research progress of MTHFR C677T polymorphism and plasma Hcy level with coronary heart diseases patients with heart failure.
2017 Vol. 14 (21): 50-53,65 [Abstract] ( 193 ) HTML (1 KB)  PDF (886 KB)  ( 113 )
53 Research progress on mechanism of comorbidity of epilepsy and Alzheimer disease
ZHAO Juan ZHANG Yanmei ZHENG Naizhi
Epilepsy and Alzheimer disease (AD) are common diseases in neurology, whose pathogenesis is complex and lack of effective treatment. The existing studies have shown that significant correlations are found in the etiology, pathogenesis, pathological changes, clinical, manifestations, treatment and other aspects of these two diseases. In recent years, researches on the two disease are mainly about the inflammation, degeneration and the function abnormalities of the neural network and disease caused by neural immune. In this paper, it reviews the research progress on the pathogenesis of epilepsy and AD from neural degeneration and abnormal network function [including: amyloid  beta protein and abnormal excitatory neural network; hippocampal sclerosis in the pathogenesis of EP and AD correlation; EP in AD patients; tau protein dependent voltage-gated potassium channel Kv4.2 (Kv4.2) deletion and dendritic hyperexcitability] and the role of immune inflammatory response in the pathogenesis of EP and AD .
2017 Vol. 14 (21): 53-56 [Abstract] ( 189 ) HTML (1 KB)  PDF (610 KB)  ( 128 )
57 Clinical research situation of asymptomatic cerebral infarction
WU Xiangju ZHANG Chunmei▲
Asymptomatic cerebral infarction (ACI) is a kind of cerebrovascular disease, which is associated with age, hypertension, carotid atherosclerosis and so on. Because of the ACI does not show typical symptoms and signs, both doctors and patients often ignore it, most of them are found by physical examination, at this time the patient's brain parenchyma has been damaged, and even irreversible pathological changes have happened. Early detection, intervention and treatment timely is extremely important. If not timely control the risk factors of ACI, it can increase the risk of cerebral infarction, neurocognitive dysfunction and mental illness. At present, ACI is detected mostly by MRI, high resolution MRI can improve the detection rate of ACI and reduce the misdiagnosis rate.
2017 Vol. 14 (21): 57-60 [Abstract] ( 138 ) HTML (1 KB)  PDF (609 KB)  ( 65 )
61 Investigation on the health status among middle-aged and old males in the rural areas of Nansha District in Guangzhou City
ZUO Liandong1 QU Yanxia1 GENG Keting1 GUO Ruizhen2 MO Linghong2 LI Honggang3 GUAN Huangtao3 TANG Linguo1▲
Objective To carry out the investigation on the status of middle-aged and old males in rural areas, and provide data support for middle-aged and old males health service model. Methods A cluster sampling method was used to investigate the current situation of middle-aged and old males in 6 villages of Nansha District in Guangzhou from December 2014 to Novembe 2015. 1134 people aged 40-79 years were selected and signing the informed consent, established archives for family health in health questionnaire, physical examination. Results ①The proportions of smoking, drinking and high purine diet in men aged 40-79 years were higher, due to poor health awareness and lack of proper evaluation and cognition of self-health. ②The physical examination showed that the proportions of obesity, hypertension, hypertriglyceridemia, high cholesterol, hyperuricemia and diabetes mellitus among the middle-aged and old males were higher than other reported, which was 12.88% (according to BMI standards), 40.79%, 22.65%, 73.98%, 43.19%, 11.15% respectively. ③The detection rate of prostate calcification, the incidence of BPH and the positive rate of PSA was 26.31%, 14.20% and 5.06%, respectively. The characteristics increased with age were consistent with those reported at home and abroad, but it also had regional characteristics. Conclusion For serious health hazard of common diseases, chronic diseases, diseases with regional characteristics among middle-aged and old males, through the questionnaire and routine census, can provide evidence-based medical basis for carrying out health education and development of health care-related services among middle-aged and old males
2017 Vol. 14 (21): 61-65 [Abstract] ( 193 ) HTML (1 KB)  PDF (478 KB)  ( 99 )
66 Comparison analysis on the physical growth and development of sport quality among Yi children and adolescents in Liangshan between 2010 and 2014
WANG Shijing1 WANG Qixing1 BIAN Shaoyong1 YOU Lu1 JI Lu1 YANG Shujuan2
Objective To compare the physical development and athletic quality of children and adolescents in Liangshan Yi Autonomous Prefecture between 2010 and 2014, understand the development situation of children and adolescents of the Yi nationality, in order to improve the healthy level of children and adolescents of the Yi nationality. Methods Based on the multistage random cluster sampling, a total of 2838 and 2871 children aged 7-18 years were selected from Xide County of Liangshan Yi Autonomous Prefecture in 2010 and 2014 respectively, and indexes regarding physical growth and development of sport quality were collected and analyzed. All the data were analyzed by SPSS 20.0 software. Results The weight, height and body mass indexes of male and female students of the Yi nationality were significantly improved in 2014 compared with those in 2010 (P < 0.05), but the vital capacity values of young ages in 2014 were significantly lower than those in 2010 (P < 0.05). The time for 50 meter running and grip strength of males in 2014 were significantly lower than those in 2010 (P < 0.05), while the standing broad jump scores of males in 2014 were significantly higher than those in 2010 (P < 0.05). The standing broad jump scores and sit-and-reach distances of females in 2014 were significantly lower than those in 2010 (P < 0.05), and the grip strength of females in 2014 were significantly higher than those in 2010 (P < 0.05). Conclusion There is a significant improvement of the Yi students' nutrition status, but the vital capacity values of young ages are reduced. Moreover, the improved level of physical growth of females is lower than that of males. Therefore, corresponding nutrition and health education strategies should be taken to improve the health levels of the Yi students.
2017 Vol. 14 (21): 66-69 [Abstract] ( 184 ) HTML (1 KB)  PDF (475 KB)  ( 109 )
70 Analysis on the clinical related influence factors of thyroid stimulating hormone in the early pregnancy of pregnant women
JIANG Qiuming ZHANG Xiyang LEI Lei
Objective To observe the relationship of serum thyroid stimulating hormone (TSH) with human chorionic gonadotropin (β-hCG), estrogen and thyroid-associated antibodies in the early pregnancy of pregnant women, and to evaluate the clinical value of TSH screening in early pregnancy. Methods Total 102 healthy pregnant women with 10-12 weeks of perinatal period treated in Aerospace Center Hospital from January to October 2016 were selected as research objects. According to the serum TSH level, they were divided into TSH≤2.5 μU/mL group and TSH＞2.5 μU/mL group. The differences of β-hCG and estrogen between the two groups and the relationship with thyroglobulin antibodies (TGAb) and thyroid peroxidase antibody (TPOAb) were compared. Results There were significant differences in the levels of β-hCG between the two groups (P ＜ 0.05), and the increase of serum TSH had a correlation with thyroid-associated antibodies. Conclusion The increase of TSH levels in early pregnancy is related to β-hCG and thyroid-associated antibodies.
2017 Vol. 14 (21): 70-73 [Abstract] ( 193 ) HTML (1 KB)  PDF (341 KB)  ( 107 )
74 The anti-reflux effectiveness of direct ureteral nipple in laparoscopic ureteral reimplantation
BAI Zunguang WANG Zhaohui DAI Ruixin ZHU Shoulun PAN Jun WU Tao
Objective To explore the anti-reflux effectiveness of direct ureteral nipple in laparoscopic ureteral reimplantation. Methods Thirteen patients (14 sides) underwent laparoscopic direct nipple ureteroneocystostomy. In each case, the lower end of the ureter was freed and delivered out of the peritoneal cavity through the ipsilateral trocar. Then the lateral ureter was tailored and folded back onto itself approximately 1 cm to make into nipple evaginated mode. A double-J stent was placed in the ureter and replaced in the abdomen. The posterolateral wall of bladder was opened. Then the nipple and double-J stent were placed into the bladder lumen and a mucosa-to-full thicknessanastomosis was made. Results Surgery was successful in all 13 patients. 13 sides monitored with cystourethrography and cystoscopy did not demonstrate vesicoureteral reflux. Cystoscopy revealed the intravesical nipple was 0.8-1.0 cm long. The shape of nipple was regular without depression and atrophy. But 1 case was revealed with third-degree reflux. Cystoscopy revealed the shape of nipple was irregular with partial depression and atrophy. The nipple remained open during the bladder-filling period. The median follow-up was 24 months (6-63 months). No patients showed the improvement of hydronephrosis or ureterectasia on ultrasound. No patients occurred acute pyelonephritis or the symptom of backache. Conclusion Laparoscopic ureteral reimplantation with direct ureteral nipple shows effective anti-reflux effectiveness. It is relatively easy to operate during the process to implant the nipple and worthy of being widely applied in clinic. However, such technical issues need to be standardized.
2017 Vol. 14 (21): 74-77 [Abstract] ( 186 ) HTML (1 KB)  PDF (541 KB)  ( 88 )
78 Effect of telectrovaporization and bipolar plasma kinetic resection in the treatment of prostatic hyperplasia
SUN Shujuan SHEN Hongmei WANG Ying▲ YU Chunhu XIN Xin LI Qin'e WANG Peipei LI Yuting
Objective To compare the clinical efficacy of telectrovaporization and bipolar plasma kinetic resection in the treatment of benign prostatic hyperplasia. Methods The clinical data of 120 patients who underwent prostate surgery in Beijing Rehabilitation Hospital, Capital Medical University from January 2013 to January 2016 were analyzed retrospectively. 60 patients treated with transurethral bipolar plasma kinetic resection were selected as the electric resection group, 60 patients treated with 2 μm laser vaporization cutting were selected as the laser group. The operation time, intraoperative bleeding, amount of irrigation fluid, postoperative bladder irrigation time, foley catheter indwelling time, time to ambulation, and average hospital stay, symptoms improvement situation of two groups were analyzed statistically. Results The international prostate symptom score (IPSS), life quality score (QOL), maximum urinary flow rate (Qmax), residual urine (RU) volume before and after operation between the two groups had no significant differences (P ＞ 0.05). The IPSS score, QOL score, Qmax and RU after operation in the two groups were all better than those before operation (P ＜ 0.01). The operation time of laser group was shorter than that of electric resection group (P ＜ 0.05), intraoperative amount of irrigation fluid and blood loss was less than that of electric resection group (P ＜ 0.01), the postoperative bladder irrigation time, foley catheter indwelling time and average hospital stay of laser group was shorter than those of electric resection group (P ＜ 0.01 or P ＜ 0.05). Conclusion Transurethral 2 μm laser and bipolar plasma kinetic resection are both effective methods for the treatment of benign prostatic hyperplasia, but transurethral 2 μm laser has shorter operative time, less intraoperative blood loss, less irrigation saline and shorter postoperative bladder irrigation time, indwelling catheter time, and hospital stay, which has obvious clinical advantages.
2017 Vol. 14 (21): 78-81 [Abstract] ( 177 ) HTML (1 KB)  PDF (471 KB)  ( 121 )
82 The effect and mechanism of extract of Folium Ginkgo in protecting the early renal injury caused by extracorporeal shock wave lithotripsy
ZHAO Junlong1 JIANG Ning2 LI Yan1 XU Jidong2 ZHANG He2 HU Chuanyi2▲
Objective To study the protective effect of extract of Folium Ginkgo in the early renal injury caused by extracorporeal shock wave lithotripsy (ESWL), and to explore it possible mechanism. Methods Eighty patients with unilateral lithotripsy pelvis/calyceal calculi underwent ESWL admitted to Shanghai Pudong New Area Gongli Hospital from January 2016 to January 2017 were randomly divided into observation group and control group, with 40 cases in each group. The observation group was administrated with Folium Ginkgo 20 mL plus normal saline 250 mL, 3 d before ESWL, intravenous drip, q12h, 5 d; the control group was administrated with normal saline 250 mL at the same time point. The differences of renal injury [urine neutrophil gelatinase-associated lipocalin (NGAL), cystatin C (Cys-C)] and the levels of serum oxygen radical [malondialdehyde (MDA), myeloperoxidase (MPO)], antioxidation [total antioxidant capacity (TAC), total superoxide dismutase (TSOD)] before and after lithotripsy in the two groups were compared. Results 24 h after ESWL, the levels of urine NGAL and urine Cys-C in observation group [(17.56±3.94), (38.29±3.84) ng/mL] were lower than those of control group [(22.68±4.82), (45.63±5.37) ng/mL], the differences were highly statistically significant (P ＜ 0.01); the levels of serum MDA and MPO [(5.05±0.84) mmol/L, (68.64±21.35) U/mg] were lower than those of control group [(5.91±1.13) mmol/L, (76.28±22.17) U/mg], the differences were statistically significant (P ＜ 0.05); the levels of serum TSOD and TAC [(147.65±43.38) kU/L, (12.58±3.34) U/mg] were higher than those of control group [(123.52±41.29) kU/L, (8.35±2.14) U/mg], the differences were also statistically significant (P ＜ 0.05, P ＜ 0.01). Conclusion The extract of Folium Ginkgo can effectively protect the renal injury caused by ESWL, the mechanism may be through enhancing the antioxidation ability and eliminating oxygen free radicals.
2017 Vol. 14 (21): 82-85 [Abstract] ( 164 ) HTML (1 KB)  PDF (483 KB)  ( 110 )
86 Clinical effect of dynamic cervical implantversus and anterior cervical diskectomy and fusion in the treatment of single-level cervical degenerative disc disease
SONG Qi GUO Weichun DAI Guo QIU Bo
Objective To evaluate clinical outcome of dynamic cervical implant versus (DCI) or anterior cervical diskectomy and fusion (ACDF) in the treatment of cervical spinal cord or cervical spondylotic myelopathy caused by single-level cervical degenerative disc disease. Methods 60 patients with single-level cervical degenerative disc disease in Renmin Hospital of Wuhan University from March 2013 to August 2014 were divided into DCI group (24 cases) and ACDF group (36 cases) according to their different surgical methods. The operation time, bleeding volume, the visual analog scale for neck (VAS-N) and the arm pain (VAS-A), the neck pain and disability scale (NPAD) and the European quality of life scale (EQ-5D), surgery adjacent segmental mobility (flexion and extension angle) and operative segmental cervical vertebra activity (ROM) before after operation were observed. Results The operation time and bleeding volume of the DCI group were significantly lower than those of the ACDF group (P < 0.05). DCI group and ACDF group were followed up for 12-18 months, 14.6 months on average. The VAS-N, VAS-A, NPAD and EQ-5D scores were statistically significant in the two groups before and after operation (P < 0.05), and the clinical outcome was significant. But there was no significant difference between the two groups (P > 0.05). There was no significant difference in the ROM of DCI group before and after operation (P > 0.05); however, the ROM of the ACDF group was increased 12 months after operation (P < 0.05), which was higher than DCI group (P < 0.05). The cobb angle in the ACDF group 3 months after operation had significant difference compared with before operation (P < 0.05), while the cobb angle in the ACDF group 12 months after operation had no significant difference compared with before operation (P > 0.05). Conclusion DCI and ACDF treatment of single segment cervical spondylosis in the short-term follow-up clinical results are satisfactory. DCI can effectively retain the activity of the surgical segment and protect the movement of the spine compared with ACDF.
2017 Vol. 14 (21): 86-89 [Abstract] ( 149 ) HTML (1 KB)  PDF (603 KB)  ( 96 )
90 Comparison of effect of proximal femoral nail anti-rotation and proximal femoral anatomic locking plate in the treatment of intertrochanteric fracture in the elderly
XIE Xiaomin1 CHEN Yufei2 ZHOU Xiaoqing1 JIA Bin1 GOU Bo1▲
Objective To compare the effect of proximal femoral nail anti-rotation (PFNA) and proximal femoral anatomic locking plate in the treatment of intertrochanteric fracture in the elderly. Methods From March 2012 to March 2016, 94 elderly patients with unstable type femur fractures for treatment in Baoji Hospital of Traditional Chinese Medicine were selected and divided into two groups by random number table, with 47 cases in each group. The patients in group A were treated with proximal femoral anatomic lock plate, and the patients in group B were treated with bone proximal nailing. The operation situation in the two groups were compared. After 12 months after operation, the occurrence of complications, hip joint function and fracture healing in the two groups were compared. Results The operation was completed without death. The incision length, operative time, intraoperative blood loss, intraoperative blood volume, postoperative hospitalization days and the load time in group B were lower than those in group A, with statistically significant difference (P < 0.05). The X-ray showed that the group A healed slowly after surgery and the plate was more easily broken, while the group B was well healed, and there was no displacement of the lateral fracture. The postoperative complication of group B was lower than that in the group A, and the overall optimal rate of clinical effect in the group B was better than that in the group A (P < 0.05). Conclusion PFNA treatment for the elderly with intertrochanteric fracture has the advantages of quick recovery, simple operation, strong stability and few complications, which is worthy of clinical application.
2017 Vol. 14 (21): 90-93 [Abstract] ( 178 ) HTML (1 KB)  PDF (673 KB)  ( 95 )
94 Fracture analysis of femoroplasty for the prevention of hip fracture in the elderly
SUN Wentao1 LI Pengfei1 LIN Ziling2 HE Xiangxin1 LI Honggeng3 LU Ronggui3 FENG Lianying4
Objective To analyze effect of bone cement intervention on osteoporotic hip fracture in elderly patients based on biomechanical simulation of femoral neck fracture before and after femoroplasty. Methods CT image data of one case of femoral neck fracture was collected. Mimics software was used to rebuild the three-dimensional finite element model of femoral. Model was imported in HYPERMESH software for meshing, defining material properties, setting the boundary conditions, loading the force curve and the role of points such as pre-processing, generated model for the control model. In the case of the same boundary conditions, the finite element grid inside the femoral neck region was redefined the material properties, and the generated model was selected as the experimental model. Then the two models were imported into the solver LS-DYNA software for operating respectively, then dynamic fracture processes and Von Mises cloud maps  were observed. Results The Von Mises cloud showed a high stress on the femoral neck fracture. There was no obvious fracture line in the femoral neck of the experimental model, and the Von Mises cloud showed a hip bone around the hip stress was significantly lower than the control model. Conclusion From the perspective of biomechanics, femoral angioplasty is feasible and practical for the prevention of osteoporotic femoral neck fractures, and provides reference for clinical application
2017 Vol. 14 (21): 94-96 [Abstract] ( 182 ) HTML (1 KB)  PDF (630 KB)  ( 66 )
97 Effectiveness and safety of different application route of Tranexamic Acid in patients undergoing primary total hip arthroplasty
FAN Ya'nan1 WEN Yangyang1 ZHANG Leilei2 LI Wenlong1 ZHU Yingjie2 LIU Youwen2
Objective To investigate the application of two different routes of administration of Tranexamic Acid on the blood loss and its safety in patients with initial unilateral total hip arthroplasty (THA). Methods 90 patients with femoral head necrosis who need to undergo initial unilateral THA in He'nan Luoyang Orthopedic Traumatological Hospital were randomly divided into three groups by STATA software from October 2014 to August 2016 in Luoyang Orthopedic Hospital of He'nan. They were group A (staticly drop Tranexamic Acid before 10 minutes of the surgery), group B (injecting Tranexamic Acid after completion of surgery) and group C (without using Tranexamic Acid). The three groups of blood loss, postoperative drainage, invisible blood loss, total blood volume, the number of blood transfusions after surgery, blood transfusion and transfusion rate, the rate of deep vein thrombosis and pulmonary embolism after one week and one month follow-up were compared. Results The quantity of postoperative drainage, invisible blood loss, total blood volume, the number of blood transfusions after surgery, blood transfusion and transfusion rate differences were statistically significant (P < 0.05) among three groups, and the blood loss in the surgery had no statistically significant difference (P > 0. 05). Patients in the group C respectively 2 and 4 days after the surgery in 1 case between limb muscle, vein thrombosis and 2 cases of limb phil superficial vein thrombosis, pulmonary embolism was not occurred, who were timely received symptomatic treatment, with leaving hospital improved, after 1 month reviewing, color Doppler ultrasound prompted that the venous thrombosis had disappeared. The patients of group A and group B had not lower limb venous thrombosis and pulmonary embolism in postoperative 1 week and followed up for 1 month. Conclusion Preoperative intravenous and retrograde injection through the drainage tube application of Tranexamic Acid can significantly reduce the primary unilateral total hip arthroplasty in patients with postoperative blood loss and transfusion rate, and the recent security is a very high, and local injections of Tranexamic Acid is more safe and effective, without postoperative adverse reactions and complications.
2017 Vol. 14 (21): 97-100,116 [Abstract] ( 172 ) HTML (1 KB)  PDF (628 KB)  ( 136 )
101 Effect of iliac bone flap pedicled with deep circumflex iliac artery with decalcified human dental matrix material in the treatment of osteonecrosis of the femeral head
ZHANG Leming FENG Jianshu▲ FU Qiang WANG Jiangyong
Objective To investigate the effect of iliac bone flap pedicled with deep circumflex iliac artery with decalcified human dental matrix material (DTM) in the treatment of osteonecrosis of the femeral head. Methods From September 2013 to October 2015, 166 patients with osteonecrosis of the femeral head from the Department of Orthopedics, Shijiazhuang the Third Hospital were selected and divided into control group and experiment group, with 83 cases in each group. The control group was treated with implantation of iliac bone flap pedicled with deep circumflex iliac artery, while the experiment group was received implantation of iliac bone flap pedicled with deep circumflex iliac artery with DTM, hip symptoms and functional score, serum osteocalcin (BGP), type Ⅰ procollagen N propeptide (PINP), type Ⅰ procollagen C terminal peptide (CTX-Ⅰ) levels, SF-36 quality of life score were compared. Results Compared with the control group, pain visual analogue scale (VAS) score in the experiment group was lower, Japanese Society of Department of orthopedics (JOA) hip function score and Harris hip score in the experiment group were higher after treatment, serum levels of BGP、PINP、CTX-Ⅰ were lower, phyisical component summary scale (PCS) and mental component summary scale (MCS) score in the SF-36 quality of life were higher, with significant difference (P < 0.05). Conclusion The clinical curative effect of iliac bone flap pedicled with deep circumflex iliac artery with DTM in the treatment of osteonecrosis of the femeral head is exactly, which can reduce the levels of serum BGP, PINP, CTX-Ⅰ and hip pain symptoms, improve the function of hip joint and life quality of the patients.
2017 Vol. 14 (21): 101-104 [Abstract] ( 167 ) HTML (1 KB)  PDF (647 KB)  ( 104 )
105 Change of interleukin-17 levels in both synovial fluid and serum of patients with medial compartment knee osteoarthritis after proximal fibular osteotomy
BAI Yuming1 ZHANG Haisen1 LIU Chang1 JIN Shengli1 WANG Wenjie2 LIU Ying3 ZHANG Yanping3 LYU Zhichang4
Objective To investigate interleukin-17 (IL-17) levels in both synovial fluid and serum of patients with medial compartment knee osteoarthritis (KOA) after proximal fibular osteotomy. Methods Thirty patients with medial compartment KOA underwent proximal fibular osteotomy at the Cangzhou Central Hospital from December 2014 to June 2015 were selected as experimental group, and 30 healthy individuals were selected as control group into the study. Visual analog pain score (VAS) and Knee Society score (KSS) were used to evaluate pain level and function of the experimental group. The IL-17 levels in both serum and synovial fluid of the experimental group at preoperative period and postoperative 6, 12, 18 months were measured by ELISA, also the serum levels of IL-17 in the control group were measured by ELISA. Results All incisions were healed in phase I. And all patients were followed up at least 18 months. The average tibiofemoral angle was (168.6±3.4)° at 18 months after surgery. Average postoperative VAS pain and KSS scores were significantly improved compared with preoperative period (P < 0.05), while there were no statistically significant differences during postoperative points (P > 0.05).The serum levels of IL-17 in the experimental group at different time points were higher than that in the control group (P < 0.05). The IL-17 levels in both serum and synovial fluid of the experimental group during postoperative points were decreased compared with preoperative period (P < 0.05), while there were no statistically significant differences during postoperative points (P > 0.05). Conclusion Proximal fibular osteotomy can significantly improve the pain symptoms and joint function of the patients with medial compartment KOA. It also can reduce the IL-17 levels in both synovial fluid and serum, while which can not back to healthy level.
2017 Vol. 14 (21): 105-108 [Abstract] ( 164 ) HTML (1 KB)  PDF (600 KB)  ( 52 )
109 Effect of laminectomy decompression and poking reduction combined with vertebral bone graft in the treatment of thoracolumbar fracture with spinal canal encroachment
SHANG Pingfu1 XU Qinhua1 WANG Guifang1 LI Dongfeng2
Objective To investigate the clinical effect of laminectomy decompression and poking reduction combined with vertebral bone graft for thoracolumbar fracture with spinal canal encroachment. Methods All of 63 patients who had thoracolumbar fracture in AO-A type accepted operation in the period of August 2010 to May 2013. They were divided into two groups based on the surgical procedure. The group A (30 cases) was accepted laminectomy decompression and poking reduction combined with vertebral bone graft. The group B (33 cases) was accepted short-segment fixation and laminectomy decompression. The operative time, intraoperative blood loss, vertebral body height, Cobb angle, and Oswestry disability index (ODI) before and one week after operation, and final follow-up were recorded and analyzed. Results All the operations were completed successfully, and all incisions were healed in phase I. The operation time and intraoperative blood loss in the group B were less than those in the group A (P < 0.05). One week after operation, the vertebral body height, local Cobb angle of the two groups were better than those before operation, and the vertebral body height, local Cobb angle in the group A were better than those in the group B (P < 0.05). At final follow-up time, in the group B, the vertebral body height was lost, and local Cobb angle was increased (P < 0.05), while group A was sustained well (P ＞ 0.05). Conclusion Laminectomy decompression and poking reduction combined with vertebral bone graft will, but can maintain the height of the vertebral body well and avoid the shell-like vertebral body occurred, although it will increase the operation time.
2017 Vol. 14 (21): 109-112,132 [Abstract] ( 140 ) HTML (1 KB)  PDF (1106 KB)  ( 144 )
113 The effect of lumbar disc herniation treated with posterior spinal fusion and instrumentation in pedicle
WEI Xiaoling1 SHE Gang2
Objective To observe the effect of lumbar disc herniation treated with posterior spinal fusion and instrumentation in pedicle. Methods 87 cases patients received treatment in Jianyang TCM Hospital from March 2013 to September 2016 of disc herniation were divided into control group (43 cases) and experimental group (44 case) by random number table. Patients in the experimental group were treated by pedicle screw fixation, while patients in the control group were treated with disc hermetination paste. The scores of JOA, ODI and VAS, clinical efficacy and adverse reactions were compared. Results There was significant difference between the two groups before and 6 months after treatment, the JOA, ODI and VAS scores in the two groups were better than those before treatment, and scores in the experimental group was superior to the control group (P ＜ 0.05). After treatment, the effect of experimental group was better than that in control group (P ＜ 0.05). The statistical difference was not statistically significant between two groups (P ＞ 0.05). Conclusion The effect of lumbar disc herniation treated with posterior spinal fusion and instrumentation in pedicle is better, which has a clinical reference value.
2017 Vol. 14 (21): 113-116 [Abstract] ( 179 ) HTML (1 KB)  PDF (639 KB)  ( 97 )
117 Clinical research of respiratory distress syndrome in late preterm infants
DING Sufang LIU Yingying WU Rong▲
Objective To investigate the incidence, risk factors, clinical features of respiratory distress syndrome (RDS) in the late preterm infants. Methods The clinical data of prematures who were hospitalized and cured in Maternal and Child Healthcare Hospital of Huaian Affiliated to Yangzhou University Medical Academy from January 2010 to December 2013 were retrospectively analyzed. All the prematures who ′s age were less than 72 hours and the gestational age were 34+1-36+6 weeks. Results Participants included 452 cases, RDS group was 152 cases and control group was 300 cases. The incidence rate of RDS in late preterm infants was 33.6% (152/452). There were no statistical significance of two groups in birth weight, gestational age, fetal time (P > 0.05). The rest of basic situation of two groups was compared, with statistical difference (P < 0.05).Incidence rate of premature rupture of membranes in RDS group was lower than that in control group (P < 0.05). Incidence rate of placental abnormality, fetal distress, CPAP, tracheal intubation, pulmonary hemorrhage, PPHN, gas leak, VAP and intracranial hemorrhage in RDS group were higher than those in control group (P < 0.05). Logistic regression analysis showed that the risk factors of RDS in the late preterm infants were premature rupture of memberane, cesarean elicery, elective caesarean section leaded by scar uterus and social factors (P < 0.05). Conclusion Late preterm infants with a higher incidence of RDS, heavier and more complications. The risk factors of RDS in the late preterm infants are premature rupture of memberane, cesarean elicery, elective caesarean section leaded by scar uterus and social factors.
2017 Vol. 14 (21): 117-120 [Abstract] ( 192 ) HTML (1 KB)  PDF (538 KB)  ( 123 )
121 Comparison of clinical application effects with different methods of induction of labor in late pregnancy
XUE Juan WANG Yuelan LI Youjuan WANG Na WANG Lixiu XIA Fengna WANG Junpu
Objective To discuss application effects with low small bladders, small dose of Misoprostol after vaginal fornix and small dose of Oxytocin intravenous drip for induced labor of late pregnancy. Methods 180 late pregnant women from April 2014 to January 2016 were selected and randomly divided into 60 patients of them accepted with foley catheter low water bladders for induced labor were taken as water bladders group, 60 patients of them accepted with small dose of Misoprostol after vaginal fornix for induced labor were taken as Misoprostol group, and another 60 patients of them accepted with small dose of Oxytocin intravenous drip for induced labor were taken as Oxytocin group. The effect of cervical ripening and induced labor, delivery outcome and birth state of newborns among three groups were observed. Results Bishop scores in water bladders group and Misoprostol group after interventions were higher than those in Oxytocin group, the differences were statistically significance (P ＜ 0.05). Ttotal effective rate of induced labour in water bladders group and Misoprostol group were higher than those in Oxytocin group, the differences were statistically significant (P ＜ 0.05). Induction of labor to labor time and total labor in water bladders group and Misoprostol group were shorter than those in Oxytocin group, the differences were statistically significant (P ＜ 0.05). Vaginal delivery rates in water bladders group and Misoprostol group were higher than those in Oxytocin group, cesarean section rate in water bladders group and Misoprostol group were lower than those in Oxytocin group, the differences were statistically significant (P ＜ 0.05). The amount of postpartum hemorrhage, newborn′s weight, amniotic fluid contamination and newborn asphyxia rate among three groups were compared, with no statistical difference (P ＞ 0.05). Conclusion Foley catheter low water bladders and small dose of Misoprostol after vaginal fornix apply to induce labor of late pregnancy with effects of cervical ripening that can increase the success rate of induced labor, reduce the cesarean section rate, which are worthy of clinical use.
2017 Vol. 14 (21): 121-124 [Abstract] ( 208 ) HTML (1 KB)  PDF (537 KB)  ( 83 )
125 The relationship of short PR interval during pregnancy and the level of thyroid hormone
LIAO Qi
Objective To investigate the incidence of the short PR interval and relationship with the level of thyroid hormone during pregnancy. Methods From January to December 2016, in Shunyi Maternal and Children Health Care Hospital in Beijing City ("our hospital" for short), 8645 cases of pregnant women and 8650 cases of non-pregnant women from physical examination were performed an electrocardiogram as study group and control group. The pregnant women with the short PR interval were divided into three groups according to different gestational age: early pregnancy group (＜14 weeks), mid pregnancy group (14-＜28 weeks) and late pregnancy group (≥28 weeks). TSH, FT3, FT4, TT3, TT4 were tested. Results The incidence of short PR interval in pregnant women was 1.504%, and incidence of control group was 0.358%, the difference was statistically significant (P ＜ 0.01). They were statistically significant in short PR interval, TSH, FT3, FT4, TT3 and TT4 among groups (P ＜ 0.01). TSH: there was no significant difference between early pregnancy group and mid pregnancy group (P ＞ 0.05). There were significant difference between early pregnancy group and late pregnancy group, mid pregnancy group and late pregnancy group (P ＜ 0.05). Short PR interval, FT3, FT4, TT3 and TT4: there were significant difference between early pregnancy group and mid pregnancy group, early pregnancy group and late pregnancy group (P ＜ 0.01). There was significant difference between mid pregnancy group and late pregnancy group (P ＜ 0.01). Conclusion The incidence of short PR interval in pregnant women is significantly higher than that of healthy non-pregnant women. The thyroid hormone level and the short PR interval can be monitored by clinical observation. It can prevent the occurrence of arrhythmia by combining clinical symptoms effectively. It is important clinical significance to improve the safety of women with short PR interval during pregnancy.
2017 Vol. 14 (21): 125-128 [Abstract] ( 158 ) HTML (1 KB)  PDF (539 KB)  ( 259 )
129 Clinical effect of Diazepam combined with Phenobarbital in the treatment of infantile convulsion
HUANG Jing1 DENG Jing2▲
Objective To analyze the effect of Diazepam combined with Phenobarbital in the treatment of infantile convulsion and the influence of serological indicators on children. Methods 91 cases with febrile convulsion treated in Jiangjin Chinese Medicine Hospital of Chongqing City from March 2013 to April 2015 were divided into control group and observation group according to the random number table. The control group was treated with Diazepam drugs administered as intravenous note. The observation group was treated with Phenobarbital based on control group. Clinical efficacy, serum parameters, prognosis and immune function of two groups were evaluated. Results Total efficacy rate of observation group was significantly higher than that of control group, the difference was statistically significant (P < 0.05). The cumulative recurrence rates at 6, 12 months in control group were significantly higher than those in observation group, the differences were statistically significant (P < 0.05). The number of patients transferinto epilepsy at 6, 12 months in control group were significantly higher than those in observation group, the differences were statistically significant (P < 0.05). After treatment, the serum level of NSE, S-100 beta, BNDF in observation group were significantly lower than those in control group, the differences were statistically significant (P < 0.05). After treatment, IgA, IgM and IgG levels in observation group were significantly lower than those in control group, the differences were statistically significant (P < 0.05). After treatment, the proportion of CD4+ in observation group was significantly higher than that in control group (P < 0.05), the proportion of CD8+ in observation group was significantly lower than that in control group (P < 0.05), the ratio value of CD4+/CD8+ in observation group was significantly higher than that in control group (P < 0.05). Conclusion The effect of Diazepam combined with Phenobarbital in the treatment of infantile convulsion is exact, can reduce serum NSE, S-100β, BNDF levels, has a good clinical application prospects.
2017 Vol. 14 (21): 129-132 [Abstract] ( 154 ) HTML (1 KB)  PDF (538 KB)  ( 61 )
133 Influence of blue light irradiation combined with abdominal circular massage on bilirubin and jaundice regression in neonatal jaundice
ZHU Yuanyuan LIU Ying▲
Objective To explore the effect of blue light irradiation combined with abdominal circular massage on neonatal jaundice. Methods Clinical data of 150 patients with neonatal jaundice in the First People's Hospital of Nantong City, Jiangsu Province from June 2015 to December 2016 were retrospectively analyzed. They were divided into two group by different treatment methods, control group (phenobarbital treatment) of 70 cases and observation group (phenobarbital, blue light irradiation combine with abdominal circular massage treatment) of 80 cases. The meconiun turning yellow time, daily defecation number, eliminating jaundice time, bilirubin, clinical effects of two groups were detected. Results The meconiun turning yellow time, eliminating jaundice time in observation group were shorter than those in control group, daily defecation number in observation group was more than that in control group, with statistical differences (P < 0.05). There was no significant difference in the levels of bilirubin before treatment between two groups (P > 0.05). The bilirubin levels of observation group after treatment of 1, 3, 7 d were lower than those of control group, with statistical differences (P < 0.05). With the continuous prolongation of treatment time, the levels of bilirubin in two groups of neonatal jaundice were gradually decreased, the difference was statistically significant (P < 0.05). Total effective rate of clinical treatment of neonatal jaundice in observation group was higher than that in control group, with statistical difference (P < 0.05). Conclusion Clinical symptom of blue light combined with abdominal circular massage in the treatment of neonatal jaundice is improved significantly, bilirubin decreased significantly, the effect is good, it is worthy of clinical promotion and application.
2017 Vol. 14 (21): 133-136 [Abstract] ( 136 ) HTML (1 KB)  PDF (534 KB)  ( 68 )
137 Clinical effect of complex trabeculectomy on glaucoma patients with thin Tenon's capsule
MA Yinghui YANG Jie FU Xiaoxiao DONG Weili
Objective To explore the effect of complex trabeculectomy for glaucoma patients with thin Tenon's capsule. Methods 63 primary glaucoma patients (63 eyes) with thin Tenon's capsule treated in Affiliated Hospital of Chengde Medical College from August 2014 to August 2016 were enrolled retrospectively in the study. All patients accepted trabeculectomy with intraoperative appropriate application of Mitomycin. All patients were observed closely after surgery and accepted early needle suture cut if necessary. The early postoperative anterior chamber formation, filtering bleb and intraocular pressure were observed. The long-term effect of 6 months of follow-up was observed. Results Postoperative 1 month, 60 cases (95.24%) were successful, including 31 cases (93.94%) with Mitomycin, 29 cases (96.67%) without Mitomycin, the difference was not statistically significant (P ＞ 0.05). Type Ⅰ filtering blebs were observed in 7 cases (21.21%) with Mitomycin, and 1 case (3.33%) without Mitomycin, the difference was statistically significant (P < 0.05). Postoperative 6 months, 52 cases (82.54%) were successful, including 30 cases (90.91%) with Mitomycin, 22 cases (73.33%) without Mitomycin, the difference was statistically significant (P ＜ 0.05). Type Ⅰ filtering blebs were observed in 10 cases (30.3%) with Mitomycin, and 2 case (6.67%) without Mitomycin, the difference was statistically significant (P < 0.05). The mean intraocular pressure at 1 week, 1 month and 6 months after operation was (12.63±2.29), (13.69±2.43), (15.33±1.88) mmHg, respectively, those were significantly decreased than that before operation, the differences were statistically significant (P < 0.05). Conclusion Complex trabeculectomy is effective for primary glaucoma with thin Tenon's capsule, early needle suture cut after operation for primary glaucoma with thin Tenon's capsule is simple and effective. It is worthy of promotion and application in primary hospitals.
2017 Vol. 14 (21): 137-140 [Abstract] ( 186 ) HTML (1 KB)  PDF (422 KB)  ( 158 )
141 Effect analysis of root canal therapy of MB2 in the maxillary first molar
HAO Zuoqi1 WU Bing2 LIU Baogang1 DING Wen3 YANG Lei4 WANG Xinhua1 YANG Fan5
Objective To investigate the clinical efficacy of treatment for the second root canal (MB2) in the maxillary first molar. Methods A total of 57 patients (57 teeth) with MB2 in the maxillary first molar treated in Department of Stomatology, Lishilu Clinic, PLA Rocket Force General Hospital from June 2010 to December 2015 were enrolled in this study. Before the operation X-ray film was used, and careful exploration and diagnosis of MB2 were necessary during the operation, then prepared and disinfected the root canal, filled the root canal using lateral pressure combined with sharp X-ray film. After the operation MB2 were filled in place according to X-ray film. Results 12 months after the review, the success rate was 89.47% with 51 successful teeth and 6 failed teeth. Conclusion X-ray film before and after the operation according to partial angle projection method combining careful exploration with root canal therapy completly during the operation will be useful to cure MB2 in the maxillary first molar.
2017 Vol. 14 (21): 141-144 [Abstract] ( 183 ) HTML (1 KB)  PDF (449 KB)  ( 355 )
145 Simultaneous determination of berberin and cepharanthine in Stephania longa L. by high performance liquid chromatography-tandem mass spectrometry
BAI Lili1 DAI Hua2▲ CHENG Sha1 KONG Dulin1
Objective To establish a method for the simultaneous determination of berberin and cepharanthine in Stephania longa L. by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The separation of the analytes were carried out on a Phenomenex Synergi Fusion-RP 80A column (2.0 mm×50 mm, 4 μm) using methanol containing 0.1‰ formic acid as mobile phase, the column temperature was 40℃, flow rate was 0.35 mL/min, gradient elution. HPLC-MS/MS was performed in multiple reaction monitoring mode via positive electrospray ionization (ESI+). Results The calibration curves showed good linearity over the tested range from 5.0 to 1000.0 ng/mL for 2 compounds (the correlation coefficients＞ 0.98).The recoveries and the relative standard deviations of berberin and cepharanthine was 101.46%-104.76%, 2.14% and 95.96%-104.39%, 4.63% respectively. The content of berberin and cepharanthine in Stephania longa L. from Wenchang, Xinglong 2 areas were 0.889, 1.07 μg/g and 7.62, 38.0 μg/g. Conclusion The method is simple, fast and highly sensitivity, which can be used to control the quality of alkaloids from Stephania longa L..
2017 Vol. 14 (21): 145-147 [Abstract] ( 156 ) HTML (1 KB)  PDF (418 KB)  ( 130 )
148 Correlation between neonate's blood concentration of Vancomycin and adverse reaction
LYU Shengxia1 LU Guozhou1 HUANG Xinwu2
Objective To study the correlation between neonate's blood concentration of Vancomycin and adverse reaction, in order to provide objective basis for clinical application of Vancomycin. Methods 160 neonates treated in Xichang People's Hospital from January 2014 to January 2016 were selected. The blood concentrations of all neonates were monitored during the Vancomycin treatment. The incidece of of adverse reaction was observed, and the correlation between neonate's blood concentration of Vancomycin and adverse reaction was analzyed. Results In the aspect of adverse reaction, the incidence of liver function damage was the highest, followed by hearing injury. There were no statistically significant differences in the incidence of adverse reactions among different blood concentrations of Vancomycin (P > 0.05). The change of the incidence of adverse reaction was not followed the increased blood concentration of Vancomycin. Conclusion Application of Vancomycin in neonates has higher incidence of adverse reaction, including liver function damage and hearing injury, but the incidence of adverse reaction is not related with the blood concentration of Vancomycin.
2017 Vol. 14 (21): 148-150 [Abstract] ( 170 ) HTML (1 KB)  PDF (351 KB)  ( 96 )
151 The theory of etiology and pathology of chronic visceral disease
ZHANG Tianmin1 LIU Jianmin2 WANG Ruolin3
This paper analyzes the knowledge and lack of traditional Chinese medicine and Western medicine for the chronic visceral disease, leading to a new understanding of acupotomology for chronic visceral disease, describes the definition, etiology and pathogenesis of acupotomy for chronic visceral disease and the ideas and methods of diagnosis and treatment for chronic visceral disease, analyzes the internal relationship between chronic visceral disease and spinal, put forward that the aim of acupotomlogic therapy is to restore the balance of the bowstring mechanics anatomical system of viscera, the treatment of acupotomology is to recovery the balance of the visceral bowstring mechanics anatomical system through the overall solution of the adhesion, scar and contracture of the bowstring part and adhesion stress part of the spinal bowstring mechanics anatomical system and head-ridge-limb bowstring mechanics anatomical system.
2017 Vol. 14 (21): 151-154 [Abstract] ( 229 ) HTML (1 KB)  PDF (499 KB)  ( 104 )
155 Discussion on theory origin and function principle of acupotomy in The Internal Canon of Medicine
HE Ningning LI Kaiping
Nine needles are formally defined by The Internal Canon of Medicine, Many of the needles that emerged are developed based on nine needles. Meridian sinew theory is formally defined by The Internal Canon of Medicine, and it is one of the important guiding thoughts of acupuncture clinical. The paper compares nine needles in The Internal Canon of Medicine with acupotomy, explores and analyzes the theoretical origin of acupotomy from the shape of the needle and the treatment of the disease, explores and analyzes the action principle of acupotomy from the point of view of the meridian sinew theory, in order to provide theoretical foundation for the clinical application and modern development of the acupotomy.
2017 Vol. 14 (21): 155-158 [Abstract] ( 165 ) HTML (1 KB)  PDF (508 KB)  ( 28 )
159 Clinical research of needle-knife combined with stellate ganglion block in the treatment of cervicogenic tinnitus
JIN Yinhua LI Shiliang SUN Ruihua ZHU Xinyue LIU Zixiang SHEN Yifeng ZHOU Qiaoyin
Objective To observe the effect of needle-knife combined with stellate ganglion block for cervicogenic tinnitus preliminary, and to provide new idea for the treatment of tinnitus. Methods The research objects were the patients with the first chief complaint as tinnitus treated in Outpatient Department of Acupuncture and Moxibustion, China-Japan Friendship Hospital from July 2014 to February 2016. 94 patients with cervicogenic tinnitus met with criteria were randomly divided into needle-knife combined treatment group (52 cases) and control group (42 cases). The needle-knife combined treatment group was taken needle-knife therapy (once a week, total 4 times) combined with stellate ganglion block (3 times per week, total 12 times) at the same time. The control group was only taken stellate ganglion block (3 times per week, total 12 times). The tinnitus scores, tinnitus classification, and the changes of vertebral-basilar artery blood flow velocity before and after treatment in the two groups were observed. Results The total effective rate of needle-knife combined treatment group was 53.85%, which was higher than that of control group (21.43%), the difference was statistically significant (χ2=10.2298, P ＜ 0.05). There were no significant differences of the vertebral-basilar artery blood flow velocity before and after treatment within the two groups (P ＞ 0.05). Conclusion The curative effect of needle-knife combined with stellate ganglion block in the treatment of tinnitus is remarkable. However, the correlation between improvement of tinnitus and vertebral-basilar artery blood flow velocity is not confirmed.
2017 Vol. 14 (21): 159-162 [Abstract] ( 221 ) HTML (1 KB)  PDF (537 KB)  ( 151 )
163 Clinical study on 4 cases with uterine rupture
JIAO Ying FU Yujing XU Caiyan
The article analyzes 4 cases diagnosed as uterine rupture in recent 5 years at Beijing Tongren Hospital, CMU, including 3 cases of compelete uterine rupture and 1 case of incompelete uterine rupture. Gstational weeks ranging from 25 weeks to 40+6 weeks. 4 cases with the typical syndrome of sudden severe abdominal pain, shock, abdominal tenderness and fetal heart sounds disappeared. Cesarean scar rupture was found in 1 case, myomectomy were found in 2 cases , unknown cause was found in 1 case (history of artificial abortion).The 4 cases underwent emergency exploratory laparotomy and uterine repair, after a series of symptomatic treatment, improved and discharged within 7 days. No case of maternal death and 3 cases of fetal death. Through comparative study of domestic and foreign literatures, on etiology, clinical characteristics, main points of diagnosis and treatment of uterine rupture it can be seen that uteru scar, uterine injuried operations are high risk factors of uterine rupture.
2017 Vol. 14 (21): 163-166 [Abstract] ( 159 ) HTML (1 KB)  PDF (356 KB)  ( 96 )
167 Research on mode of traditional rehabilitation therapy on work process
HONG Qiaoyu1 BU Xunsheng1▲ WANG Kerong1 LI Jiang1 WANG Jing1 WANG Bin1 JIANG Liyun2 YU Hui1
This article analyzes the present situation of talent cultivation of traditional rehabilitation therapy, puts forward the feasibility to develop and innovate the teaching mode of traditional rehabilitation therapy, crystallize the advantages of the use of micro material in the teaching of traditional rehabilitation therapy,investigate the mode of traditional rehabilitation therapy based on work process.
2017 Vol. 14 (21): 167-170 [Abstract] ( 146 ) HTML (1 KB)  PDF (685 KB)  ( 139 )
171 Soul ward round developed by sympathetic technique based on humanistic care
PENG Xiang1 LIU Yinghua2 CHEN Lianghua3 LIU Yuping2 LI Xiaoxia2 HUANG Jiongzhou2 YANG Weiwen2
After registration, patients are to be in procedure of ward round by doctors everyday clinically. Few doctors communicate with patients soul-by-soul even if they play guiding roles of direction during the ward round. In sequence, we propose a new pattern so called soul ward round in humanistic medicine developed by sympathetic technique based on humanistic care. During this soul ward round, patients usually act as principals of depiction, while doctors act as soul helpers accompanying patients to let them narrate more. The synopsis and method includes self-sympathy, patient-sympathy and sympathy on suitable options. Through soul ward round practiced by these techniques, the doctors feel patients' most real perception by heart, and provide care and assistance so that the patients are courage by confidence while considering themselves to be primary act, and the medical pattern of patient-centralized based on human being is realized in the reality.
2017 Vol. 14 (21): 171-174 [Abstract] ( 158 ) HTML (1 KB)  PDF (687 KB)  ( 96 )
175 Meta analysis on the influence of traditional Chinese medicine treatment for the hemorheology indexes of patients with diabetic retinopathy
CHEN Qiuye MA Yanyun TANG Hong
Objective To compare the influence of traditional Chinese medicine treatment and single western medicine treatment for the hemorheology indexes of patients with diabetic retinopathy by meta analysis. Methods The databases of PubMed, Medline, CBM disc, Cochrane library, CNKI, VIP, Wanfang, China Master's Theses Full-text Database and proceedings of the academic conference were retrieved. The retrieval time limit was from January 2000 to October 2016. The related clinical randomized controlled trials were researched, and the enrolled literatures were taken quality assessment and data extraction, and Revman5.3 software was used to take Meta analysis. Results In total, 26 clinical randomized controlled trials and 2421 patients were enrolled, with 1307 cases in experimental group and 1114 cases in control group. The levels of whole high blood viscosity (200/s) (SMD = -0.63, 95%CI: -0.91 - -0.34, P ＜ 0.01), whole low blood viscosity (5/s) (WMD = -0.76, 95%CI: -1.02 - -0.50, P ＜ 0.01), plasma viscosity (SMD = -0.62, 95%CI: -1.02 - -0.22, P = 0.002), fibrinogen (SMD = -0.67, 95%CI: -0.80 - -0.54, P ＜ 0.01), red blood cell aggregation index (SMD = -0.96, 95%CI: -1.46 - -0.47, P = 0.0001) in the experimental group were significantly lower than those of control group, the fundus changing curative effect (RR = 1.22, 95%CI: 1.17 - 1.28, P ＜ 0.01) and TCM syndrome curative effect (RR = 1.55, 95%CI: 1.18 - 2.03, P = 0.002) were significantly better than those of control group. The whole middle blood viscosity (30/s) between two groups had no statistically significant difference (MD = -1.22, 95%CI: -2.66 - 0.21, P = 0.10). Sensitivity analysis showed that the results were stable and reliable. Conclusion Traditional Chinese medicine treatment can reduce the whole high blood viscosity (200/s), whole low blood viscosity (5/s), plasma viscosity, fibrinogen, erythrocyte aggregation index of patients with diabetic retinopathy, and improve the fundus changing curative effect and TCM syndrome curative effect, all of which are superior to those of single western medicine treatment, while it has no effect on the whole middle blood viscosity (30/s). This paper shows that traditional Chinese medicine treatment for diabetic retinopathy has certain creative effect, but the methodological quality remains to be further improved, which needs more high-quality randomized, double-blind, controlled literatures to confirm.
2017 Vol. 14 (21): 175-180,184 [Abstract] ( 178 ) HTML (1 KB)  PDF (1532 KB)  ( 93 )
181 Analysis of outpatient narcotics medical record writing situation in our hospital and its countermeasure
CHEN Jinfeng ZHU Qingmei ZHONG Ming WANG Yubo LIU Bing▲
This article retrospectively investigates the outpatient narcotics medical record writing situation in the First Affiliated Hospital of Xinjiang Medical University From January 2014 to December 2015, analyzes the types of non-standard narcotics medical record, adopts corresponding countermeasures, through literature review, reference of cancer normalized pharmacy's medical history writing, and communication with clinical, designs new version of outpatient narcotics medical record in accordance with reality of our hospital, and puts into use. After train of outpatient clinical physicians and pharmacy pharmacists, this article investigates new version record non-standard writing from June to December 2016 again. Through comparsion between before and after, after adopting the new policy, non-standard medical record writing is decreased obviously, which improve the standard of outpatient narcotics medical record writing in our hospital.
2017 Vol. 14 (21): 181-184 [Abstract] ( 160 ) HTML (1 KB)  PDF (790 KB)  ( 98 )
185 Strategy research on transmission of wushu boxing in traditional health-care P.E. course of universities of Chinese medicine
SHI Zhenwen WANG Bin
This article, in the perspective of communication, studies the wushu boxing transmission in the traditional health-care P.E. course of universities of Chinese medicine by means of the communication principle and the demand characteristics of college students'. The thesis let wushu boxing get further promotion among college students. There were 4 basic elements in wushu boxing transmission, namely, disseminator, content, mode and communication target. Disseminators are mainly college wushu teachers and wushu boxing management department; the contents are wushu boxing. The transmitted mode is mainly interpersonal communication, organizational communication, mass communication, and network communication, etc. The communication targets are mainly for contemporary college students. The article puts forward that strengthening wushu boxing performance, holding wushu boxing contests, launching the teaching of wushu boxing, strengthening the propaganda, setting wushu boxing grades; attaching great importance to the wushu boxing technology, using opportunely of wushu boxing, carring forward the wushu boxing innovation; enhancing wushu boxing theory; intensifying wushu boxing morality; digging the connotation of wushu boxing. In the wushu boxing propagation, it can achieve good communication effects by integrating a variety of mode of transmission, using of communication strategies, fully mobilizing various aspects strength, broadening the channels, and propagating all-round multi-level propagated.
2017 Vol. 14 (21): 185-188 [Abstract] ( 142 ) HTML (1 KB)  PDF (635 KB)  ( 107 )
189 Discussion on elements of clinical teaching management in Chinese medical hospitals
LANG Senyan ZHANG Qing
Clinical teaching is an important stage in the process of medical students training. The quality of clinical teaching directly affects the quality of medical students training. To strengthen the clinical teaching management and carry out clinical teaching research is of great significance in improving the quality of clinical teaching and training medical personnel. According to the clinical teaching in Chinese medical hospital, five key elements should be carried. Improvement of clinical teaching management system, strengthen the construction of teaching staff, ensure the teaching funds, standardize the clinical teaching process management, pay attention to clinical teaching evaluation. Finally improve the teaching methods and the model of clinical theory and skills and promote the quality of clinical teaching and medical students training.
2017 Vol. 14 (21): 189-192 [Abstract] ( 157 ) HTML (1 KB)  PDF (632 KB)  ( 133 )
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