中国医药导报
      Home                                                                                    中文
中国医药导报
 
Forthcoming Articles
Current Issue
Archive
Advanced Search
Read Articles
Download Articles
Email Alert
 
Quick Search  
  Adv Search
2017 Vol. 14, No. 33
Published: 2017-11-25
 
 
4 Construction and application of LOXP-DsRed-LOXP system
SONG Dan ZHENG Yongbin▲ TONG Shilun XIAO Kuang YANG Chao SUN Wei QIN Kaidi
Objective To Establish a cell line contains the LOXP-DsRed-LOXP system, in order to study its application in detecting the activity of Cre recombinase in vitro and in vivo. Methods MCA38LOXP-DsRed-LOXP cells were established to validate its ability of detecting the activity of Cre recombinase in vitro. Balb/c nude mice were randomly divided into two groups (n=10). MCA38Cre-Luciferase and MCA38Luciferase cells were transplanted in colorectal mucosa respectively and then collected enema two week later to validate its ability of detecting the activity of Cre recombinase in vivo. APCLOXP/LOXP mice were randomly divided into two groups (n=20). Lentivirus expressing Cre and luciferase enzyme or expressing luciferase only were injected to colorectal mucosa respectively. Enema were collected at the 3rd day and 3 weeks later the activity of Cre recombinase was detected. Meanwhile, the in vivo imaging of mice was applied to detect the expression of luciferase. The application of this system in detecting the activity of Cre recombinase and predicting the success rate of gene modification was verified. Results The results showed that the red fluorescence was significantly faded in MCA38 LOXP-DsRed-LOXP cells which was modificated by Cre recombinase for more than 48 hours in vitro. Besides, the red fluorescence of MCA38LOXP-DsRed-LOXP cells treated with nude mice enema for more than 48 hours was significantly faded in MCA38Cre-Luciferase group. In group CL, Luciferase and Cre recombinase were positive in 16 mice at the 3rd day and 8 mice at the third weekend after injecting Lentivirus. In group L, Luciferase was positive in 17 mice at the 3rd day and 8 mice at the third weekend after injecting Lentivirus, while Cre recombinase was negative in all. Conclusion LOXP-DsRed-LOXP system are successfully constructed. The in vivo and in vitro non-invasive detection of Cre recombinase activity and expression was achieved. By which it could evaluate the efficiency of virus infection and gene modification of colorectal mucosal basal stem cells in APCLOXP/LOXP mice.
2017 Vol. 14 (33): 4-8 [Abstract] ( 372 ) HTML (1 KB)  PDF (799 KB)  ( 137 )
9 Effects of Yishen Quzhuo Decoction on the expression of Ghrelin, SHBG and VF in rats with PCOS-IR
ZHANG Xiaodan1 CAI Yun2 XU Xin1▲
Objective To observe the effects of Yishen Quzhuo Decoction on the expression of Ghrelin, sex hormone-binding globulin (SHBG) and visfatin (VF) in rats with polycystic ovarian syndrome-insulin resistance (PCOS-IR). Methods Eighty-four female SD rats aged 6-week old were completely randomized into 7 groups, with 12 rats in each group, the groups were as followed: blank group, model group, high dose of Yishen Quzhuo Decoction group, middle dose of Yishen Quzhuo Decoction group, low dose of Yishen Quzhuo Decoction group, Yishen group and Metformin group. After the model was established successfully, different doses of Yishen Quzhuo Decoction or Yishen Decoction were given by gavage (high dose of Yishen Quzhuo Decoction group: 6460 mg/kg; middle dose of Yishen Quzhuo Decoction group: 3230 mg/kg; low dose of Yishen Quzhuo Decoction group: 1615 mg/kg; Yishen group: 1400 mg/kg; blank group: normal saline), Metformin was taken as control, the level changes of Ghrelin, SHBG and VF in rats of each group after medication were observed. Results The level of Ghrelin in the model group was significantly lower than that of blank group (P ＜ 0.05), while the levels of Ghrelin in each dose of Yishen Quzhuo Decoction group and Yishen group were significantly higher than that of model group, the differences were statistically significant (P ＜ 0.05). The level of SHBG in the model group was significantly lower than that of blank group (P ＜ 0.05), while the levels of SHBG in each treatment group were significantly higher than that of model group, the differences were statistically significant (P ＜ 0.05). The level of VF in the model group was significantly higher than that of blank group (P ＜ 0.05), while the levels of VF in each treatment group were significantly lower than that of model group, the differences were statistically significant (P ＜ 0.05). The levels of testosterone (T), luteinizing hormone/follicle stimulating hormone (LH/FSH), fasting insulin (FINS) were significantly higher than those of blank group (P ＜ 0.05), the levels of T, LH/FSH in each dose of Yishen Quzhuo Decoction group were lower than those of model group, the level of T in Yishen group was lower than that of model group, the levels of FINS in each dose of Yishen Quzhuo Decoction group and Metformin group were lower than that of model group, the differences were statistically significant (P ＜ 0.05). Conclusion Yishen Quzhuo Decoction can improve the factors related to insulin resistance of rats with PCOS-IR, inhibit the increase of androgen synthesis, restore the balance of glycometabolism and lipid metabolism, contain the vicious circle of PCOS-IR, so as to achieve the purpose of treating PCOS-IR.
2017 Vol. 14 (33): 9-12,21 [Abstract] ( 256 ) HTML (1 KB)  PDF (612 KB)  ( 107 )
13 Effects of Yupingfeng San and Siwu Decoction on seven kinds of bacteria in rats with chronic obstructive pulmonary disease
PENG Jing YANG Zhongshan LI Yuqing YUAN Jiali
Objective To observe the effect of Yupingfeng San and Siwu Decoction on Klebsiella pneumoniae, Pseudomonas aeruginosa, Haemophilus influenzae, Enterobacter, Streptococcus, Neisseria and Lactobacillus in rats with chronic obstructive pulmonary disease (COPD), in order to provide experimental basis for Yupingfeng San and Siwu Decoction in the treatment of COPD. Methods Fifty SPF male wistar rats were divided into 5 groups, incliding normal group, model group, Yupingfeng San and Siwu Decoction high dose, middle dose and low dose groups, with 10 cases in each group. The COPD rat model was established by intratracheal instillation of lipopolysaccharide plus smoked methods. After molding, the rats in the dose groups were given to different concentrations of Yupingfeng San and Siwu Decoction by gavage, while the rats in the normal group and model group were fed with sterile distilled water, once time a day for 30 days. All the lung tissues of rats were collected, to for high-throughput sequencing of various bacteria, and the left lung tissue was used to make pathological sections. Results ①Compared with the normal group, the content of Lactobacillus in the model group was significantly decreased, and the contents of the other six bacteria were significantly increased, with statistically significant difference (P < 0.05).②Compared with model group, the contents of Klebsiella pneumoniae, Pseudomonas aeruginosa in the Yupingfeng San and Siwu Decoction high, middle and low dose groups were significantly reduced, the contents of Haemophilus influenzae, Enterobacter, Streptococcus, Neisseria in the high dose group were significantly reduced, with statistically significant difference (P < 0.05), but there was no significant difference between the middle and low dose groups (P > 0.05).③The contents of Lactobacillus in the Yupingfeng San and Siwu Decoction low and middle dose groups was significantly increased, compared with model group, with statistically significant difference (P < 0.05), there was no significant difference between high dose group and model group (P < 0.05). Conclusion The contents of Klebsiella pneumoniae, Pseudomonas aeruginosa, Haemophilus influenzae, Enterobacter, Streptococcus and Neisseria can be significantly reduced in rats with COPD by  Yupingfeng San and Siwu Decoction, the content of Lactobacillus increasing, which may be one of the important mechanisms for the treatment of COPD .
2017 Vol. 14 (33): 13-16 [Abstract] ( 302 ) HTML (1 KB)  PDF (785 KB)  ( 149 )
17 Study on the mechanism of Metformin in the treatment of polycystic ovary syndrome
LU Chenyu QIN Peigang DU Hanyu WU Haixia Aihemaiti·Guliyaer AI Lixia XU Qi
Objective To investigate the therapeutic mechanism of Metformin on Treg cells and Th17 cells in polycystic ovary syndrome (PCOS). Methods 45 female Wister rats were gavaged by Letrozole in order to develop PCOS, and randomly chosen 5 rats to confirm the establishment of model. Then rats were divided into Metformin group [300 mg/(kg·d)], Diane group [180 mg/(kg·d)], Metformin [300 mg/(kg·d)] combined with Diane [180mg/(kg·d)] group and model group, randomly, each group had 10 rats. The other 10 female rats were arranged as control group. PCOS rats were given corresponding drugs, while control group were administrated with physiological saline, the treatment period of all groups were 28 days. The weight changes of rats weekly were observed. Ovarian histological changes were examined by Yihong-hematoxylin staining (HE). Flow cytometry was applied to delineate the phenotype of Treg cells and Th17 cells in spleen. ELISA was used to measure IL-6 and TGF-β in serum. The expression levels of Foxp3 and ROR-γt were measured by RT-PCR. Results The weight, the Treg and Th17 in spleen, Treg/Th17 ratio, the levels of IL-6 and TGF-β in serum, the expression of Foxp3 of PCOS model group were higher than control, with statistically significant difference (P < 0.05). The weight of Metformin group, Diane group and combined group were similar to control group (P > 0.05), but significantly lower than model group (P ＜ 0.05). The cystic dilated follicle in Metformin group, Diane group and combined group decreased significantly compared with the model group. Treg cells and Th17 cells in spleen, IL-6 and transcription factor ROR-γt in serum of the Metformin group were significantly higher than the model group (P ＜ 0.05). Th17 cells in spleen and IL-6 in serum of Diane group and the combined group were significantly lower than the model group (P ＜ 0.05), but the expressions of transcription factor Foxp3 in ovarian tissue were significantly higher than the model group (P ＜ 0.05). Conclusion Metformin treatment of PCOS may affect the number and proportion of Treg and Th17 cells by affecting the expression level of the serum IL-6, which can improve the microenvironment of ovarian tissue.
2017 Vol. 14 (33): 17-21 [Abstract] ( 306 ) HTML (1 KB)  PDF (748 KB)  ( 145 )
22 A preliminary study on the cross blood-brain-barrier transport of exosomal microRNA-135a
LIU Chengeng1 HAO Ting2 YANG Tingting1 MENG Shuang3 WANG Peichang1
Objective To study the effect of microRNA-135a (miR-135a) on blood-brain barrier and cell transport in exocrine. Methods The high miR-135a exosomes were extracted from the cerebrospinal fluid (CSF) of the transgenic mice and injected into the ventricles of the wild type mice and intervened with SH-SY5Y cells. The levels of miR-135a in the peripheral blood of mice and the exosomes of the culture, and the expression and activity of β-secretase-1 (BACE-1) in SH-SY5Y cells were determined. Results Intraventricular injection of miR-135a exosomes resulted in a significant increase in CSF and plasma exosomal miR-135a in wild-type mice (P < 0.05). The activity of BACE-1 in SH-SY5Y cells treated with exosomes harvest from the CSF of APP/PS1 double transgenic mice was significantly lower than that in other intervention group and control group (P < 0.05), and the mRNA expression level of SH-SY5Y cells was significantly lower than that of control (P < 0.05). The trend of change is consistent with the trend of activity. Conclusion The signal of "high miR-135a" can be transferred from CSF to blood through the blood-brain barrier, which provides a more solid experimental basis for the use of exosomal miR-135a as a biomarker of Alzheimer's disease.
2017 Vol. 14 (33): 22-25,30 [Abstract] ( 660 ) HTML (1 KB)  PDF (929 KB)  ( 308 )
26 Effects of Bawei Longzuan Granules in rats with adjuvant arthritis
LUO Yudong1,2 WU Yuqiang1 PANG Yuzhou2,3 TAN Anqiang1 MI A'na3
Objective To study the effects of Bawei Longzuan Granules in rats with adjuvant arthritis. Methods Sixty SD rats were randomly divided into blank control group, model group, Tripterygium Wilfordii Tablets group, and low, medium, high dose of Bawei Longzuan Granules group. Except for blank control group, the other groups were only given 0.1 mL of Freund complete adjuvant (FCA) injection into the left-hind feet of each rat. At the eighth day of the experiment, intragastric administration was started, once a day, the dosing volume was 10 mL/kg, the dosages of Bawei Longzuan Granules were 5.9, 11.8, 23.6 g/kg, the dosage of Tripterygium Wilfordii Tablets was 0.009 45 g/kg, blank control group and model group were given same volume of distilled water, continuous intragastric administration for 21 days. The conditions of ankle joint swelling and pathologic changes in model rats with adjuvant arthritis were observed, and enzyme-linked immunosorbent assay (ELISA) was used to detect the contents of interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α) in serum and joint tissue homogenate of rats. Results Compared with blank control group, started from the eighth day of making model, the feet thickness of model group and all medication groups was increased, the differences were statistically significant (P ＜ 0.01 or P < 0.05); compared with model group, the swelling degree of toes in high dose of Bawei Longzuan Granules group at the 29th day of modeling was decreased, the difference was statistically significant (P ＜ 0.05); the pathological examination showed that the proliferative degree of synovial cells and the invasive degree of inflammatory cells in high dose of Bawei Longzuan Granules group were all reduced; there were no statistically significant differences of the contents of serum IL-1β, TNF-α in each dose of Bawei Longzuan Granules group compared with those of model group (P ＞ 0.05). Conclusion Bawei Longzuan Granules can decrease the swelling degree of toes in rats with adjuvant arthritis, reduce the proliferation of synovial cells and inflammatory cell infiltration, which has certain improving effects for the pathological model of rats with adjuvant arthritis.
2017 Vol. 14 (33): 26-30 [Abstract] ( 270 ) HTML (1 KB)  PDF (888 KB)  ( 106 )
31 Advances in anti-inflammatory mechanism of berberine
LI Hongmei1 DANG Wantai2，3 YANG Xiaohong1
Berberine is an isoquinoline alkaloid. It was used as a broad-spectrum antimicrobial agent in the clinical treatment of pseudomembranous enteritis, chronic gastritis, peptic ulcer and pneumonia. In recent years, with the pharmacological effects of berberine research, its anti-inflammatory mechanism has gradually become a hot research. In this paper, the clinical anti-inflammatory mechanism and research status of berberine in recent years were briefly reviewed, which provided the theoretical basis for the anti-inflammatory mechanism of berberine.
2017 Vol. 14 (33): 31-34 [Abstract] ( 420 ) HTML (1 KB)  PDF (618 KB)  ( 285 )
35 Study on the influencing factors of sub-health
LIN Yuanqi1 XU Jun1▲ LI Wenyuan2 XIA Cong1 CHU Xueqin1 XIANG Qian1 WU Weixuan1
This paper analyzes the domestic and international methods to carry out the study of the influencing factors of sub-health, summarizes the influence factor of sub-health and the factors affecting the way resulting in sub-health, in order to lay a theoretical foundation for investigation study design of influence factor of sub-health. This paper analyzes the recent research literature related influence factors of sub-health, summarizes them from the definition of the concept of sub-health, the influence factor analysis method and result in sub-health by several ways, and so on. The results show that there are few articles study the sub-health influencing factors of the susceptible population, and the analysis model of sub-health is not deep enough. Therefore, a large number of epidemiological investigations, diagnostic criteria for sub-health status, and the establishment of a scientific and rational analysis model of the influence factors are the future directions of the study of sub-health factors in academia.
2017 Vol. 14 (33): 35-39 [Abstract] ( 366 ) HTML (1 KB)  PDF (666 KB)  ( 202 )
40 Research progress of interaction of thyroid disorders and trace elements
CHENG Guilin1 LI Guidong2 WANG Jianfeng1
Thyroid is one of the most important endocrine glands, and its primary function is to synthesize thyroid hormone. In recent years, the incidence of thyroid diseases has gradually increased, which mainly related to the secretion and metabolism of thyroid hormone. It is important that trace elements will regulate or affect the metabolism of thyroid hormone through different mechanisms. This article summarizes the interaction of thyroid disorders and trace elements, based on the three disorders as thyroid dysfunction, inflammation and carcinoma. The mutual correlation between them may provide a certain reference value for prevention and treatment of thyroid disorders.
2017 Vol. 14 (33): 40-43 [Abstract] ( 361 ) HTML (1 KB)  PDF (613 KB)  ( 141 )
44 Research progress of animal models of intracranial aneurysms
LI Li1* WU Xuejia2* FU Hao1 WANG Zhihong1 CHEN Dong3 SUN Li1▲ ZHANG Fang4▲
Intracranial aneurysm (IA) is a tumor like process caused by pathological and localized dilatation of intracranial arteries, once ruptured, it can result in subarachnoid hemorrhage and cause serious consequences. At present, the pathogenesis of IA has yet to be clarified, which result in that onlypatients with high risk of rupture can be treated. Many IA patients have to bear the risk of IA rupture at any time. Therefore, clarifying the mechanism of IA and developing preventive and therapeutic drugsis the key to solve the above problems. Because of the difficulty of obtaining human tissue, IA animal model has become the main research approach. This article will review the progress of IA animal model construction methods, pathological changes and drug treatment, in order to provide reference for further research.
2017 Vol. 14 (33): 44-46 [Abstract] ( 271 ) HTML (1 KB)  PDF (655 KB)  ( 109 )
47 Research progress of echocardiography for the evaluation of left ventricular systolic function in coronary heart disease
ZHANG Ling MU Yuming▲
The accurate determination of left ventricular function is important for judging the prognosis of patients and the development of clinical treatment programs. Echocardiography has the advantages of low cost, non-invasive and easy to operate and repeatable. It has become the first choice of clinical evaluation of cardiac function and synchronicity. With the rapid development of ultrasound technology, a variety of new technologies to evaluate left ventricular function are also changing rapidly, of which, speckle tracking technology is particularly prominent. This paper reviews the recent advances of new echocardiographic techniques in the evaluation of left ventricular systolic function and synchronization.
2017 Vol. 14 (33): 47-50 [Abstract] ( 318 ) HTML (1 KB)  PDF (661 KB)  ( 133 )
51 Advances in the study of PD-1/PD-L1 in triple-negative breast cancer
FAN Yu QIAN Zhen SU Qinjun LI Tiantian LIU Bin▲
Immunotherapy is one of the most popular research fields, and has proved to be effective by many clinical research centers in tumor drug trials. Program-med death receptor ligand 1 (PD-L1) is a molecule involved in immunosuppression pathway, and express in more solid tumor cells and the peripheral T lymphocyte significantly on the surface. It can combine with the programmed death receptor 1 (PD-1), inhibit the immune micro-environment around the tumor tissue, promote the deterioration of the tumor cells. Triple-negative breast cancer(TNBC), as a sub-type of breast cancer, is not sensitive to breast cancer treatment, the prognosis and life quality of patients are poor. This paper elaborates the progress in clinical trials of PD-1/PD-L1 expression and related inhibitory drugs in TNBC, and provides new ideas for the treatment of individual patients with TNBC.
2017 Vol. 14 (33): 51-54,63 [Abstract] ( 401 ) HTML (1 KB)  PDF (957 KB)  ( 240 )
55 Research progress of acupotomy treatment guided by ultrasound
SHEN Yifeng ZHOU Qiaoyin LI Shiliang▲
Ultrasound-guided technique can be widely used in the operation of acupotomelysis, nerve stimulation and needle-knife combined with drug injection, which has special advantages in the diagnostic accuracy and operational safety. This paper reviews the application status and development of acupotomy treatment guided by ultrasound, so as to provide reference and basis for clinical treatment.
2017 Vol. 14 (33): 55-58 [Abstract] ( 424 ) HTML (1 KB)  PDF (654 KB)  ( 115 )
59 Investigation and analysis of epidemiological characteristics and correlative risk factors of stroke in rural and urban communities of Hainan Province
MA Fei1 CHEN Tao2 LIU Yanhui2 PENG Xia3 ZHENG Yingying3 XIAO Jianhong3 SHENG Qing3 DENG Yidong2
Objective To investigate the epidemic characterization and risk factors of elderly residents with stroke in rural and urban communities of Hainan Province, in order to provide scientific basis for formulating prevention and intervention measures for stroke. Methods Cluster random sampling was used to investigate 18 000 residents over 40 years old in 3 urban communitis and 3 rural communitis in Hainan Province. The demographic characteristics were recorded. Stroke incidence and the stroke risk factors were analyzed. Results The prevalence rate of strok among the 18 000 individuals was 0.72%, the prevalence of stroke in urban communities was significantly higher than that that in rural communities (0.87% vs 0.56%), and the high risk population of stroke in urban communities was significantly higher than that in rural communities (10.32% vs 6.58%). Compared with the residents in rural community, there were much more residents got systemic treatment for stroke, transient ischemic attack, hypertension, hyperlipidemia, diabetes mellitus and atrial fibrillation in urban communities, the differences were highly statistically significant (P < 0.01). The rate of self-care after acute stroke of rural residents was far lower than that of urban residents (43.18% vs 75.00%), the difference was highly statistically significant (P < 0.01). Conclusion The prevalence of stroke of middle-aged and old residents in Hainan Province is significantly lower than the national averaging. The prevalence of stroke and risk factors of urban residents in Hainan are significantly higher than those in rural areas. The prognosis of stroke patients in rural areas is relatively poor, and the disability rate is higher than that in urban community patients. The most rural residents in Hainan Province are highly short of the knowledge of prevention and treatment of stroke. It is suggested that awareness of risk factors and correlated knowledge of stroke and regular physical examination should be popularized to prevent stroke in rural population.
2017 Vol. 14 (33): 59-63 [Abstract] ( 312 ) HTML (1 KB)  PDF (567 KB)  ( 245 )
64 Investigation of the community rehabilitation service ability of Tianhe District in Guangzhou City
CHEN Lianghua LIU Gang
Objective To investigate the community rehabilitation service ability of Tianhe District in Guangzhou City, in order to provide the basis for community rehabilitation service ability construction. Methods 30 community health service centers were selected to investigate the medical personnel, rehabilitation facilities, and rehabilitation treatment methods from September to November 2016. A sample survey was also performed to investigate the current rehabilitation status of patients with stroke in these communities. Results Complete survey reports were obtained from 25 centers, of which only 1 center did not offer rehabilitation services. There were 131 cases of rehabilitation medical staffs, of which the rehabilitation physician accounting for 51.91%, rehabilitation therapists accounting for 32.06%, and rehabilitation nurses accounting for 16.03%. The surveyed community health service centers had rehabilitation physicians with intermediate and junior titles accounting for 70.83% and 83.33%, respectively. Fewer rehabilitation physicians had senior title. The titles of most rehabilitation physicians were junior titles below, accounting for 20.83% of the communities. All community health service centers had physical therapy and traditional rehabilitation programs. However, only 4.17% of speech-swallowing-therapy programs was carried out. Furthermore, 87.50% of information campaigns and education programs for stroke rehabilitation were carried out by community health service centers. Thus, only 29.17% of the rehabilitation training programs for the disabled was performed. 19 patients with stroke were selected by sampling survey, 68.42% continued rehabilitation training at home, and 57.89% were admitted to relevant professional institutions for rehabilitation. Conclusion At present, the community rehabilitation service ability has some foundation in Tianhe District of Guangzhou City, but it is difficult to obtain better rehabilitation services of stroke patients. Therefore, communities should provide attention to the establishment of community rehabilitation services, promote rehabilitation awareness, and develop traditional Chinese and Western medicine community rehabilitation services.
2017 Vol. 14 (33): 64-68 [Abstract] ( 291 ) HTML (1 KB)  PDF (555 KB)  ( 133 )
69 Early beta-blocker use in acute myocardial infarction in Central-Western Urban China in 2001, 2006, 2011
ZHANG Lihua ZHANG Haibo BAI Xueke HUO Xiqian LIU Jiamin JIANG Lixin
Objective To assess trends of early beta-blocker use during hospitalization for ideal patients with acute myocardial infarction (AMI) and to analyze factors associated with not being treated in Central-Western Urban China from 2001 to 2011. Methods Two-stage random sampling design was used to create a representative sample of patients in Central-Western Urban China admitted to hospital for AMI. In the first phase, a simple random-sampling procedure was used to identify participating hospitals. In the second stage, a systematic sampling approach was applied for selecting patients admitted to each participating hospitals for AMI in 3 years (2001, 2006 and 2011). Then clinical information was obtained via central medical record abstraction for each patients. Weight calculation was used for each year to represent the overall situation. Logistic regression model was used to identify factors associated with early beta-blocker use. Results Finally 705 ideal candidates were included in study. Beta-blocker was used 55.5% in 2001, 74.6% in 2006, and 62.6% in 2011 (P=0.5927 for trend). Chest discomfort (OR=2.22，95% CI: 1.21-4.08) was found as the predictor of non-treatment. Conclusion The use of early beta-blocker therapy in Central-Western Urban China is suboptimal for ideal AMI patients who could definitely benefit from it. Patterns of use have not changed over time. It is urgent to implement strategy to improve clinician guidance adherence and quality of care for AMI.
2017 Vol. 14 (33): 69-73 [Abstract] ( 317 ) HTML (1 KB)  PDF (666 KB)  ( 119 )
74 Cognitive investigation of chronic obstructive pulmonary disease and pulmonary rehabilitation management in community nurses
LI Chunyu1 TANG Xuelian1▲ NI Wenqiong2 ZENG Jinwu1 WAN Zhiying3 WANG Xiaoyan4
Objective To know the awareness of chronic obstructive pulmonary disease (COPD) and pulmonary rehabilitation knowledge in grassroots community nurses. Methods In December 2016, self-edited questionnaire was used to investigate the awareness of COPD and pulmonary rehabilitation knowledge in 77 community nurses in Jingzhou City. Results Nobody knew the assessment of COPD patients with the modified version of the medical research council dyspnea index (mMRC), the methods of rehabilitation included exercise training, smoking cessation, nutrition consultation and education. Nobody knew the assessment of oxygenation status with pulse oximeter, and the standard and methods of breathing exercises and long term oxygen therapy. 93.51% community nurses did not know the assessment of the health and life status of COPD patients with chronic obstructive pulmonary disease assessment (CAT). 88.31% community nurses did not know that the 6-minute-walking test could be used to assess the effectiveness of impaired exercise tolerance and lung rehabilitation in patients. Conclusion The community nurses lack the knowledge of COPD and pulmonary rehabilitation. It is necessary to promote the train for community nurses to improve their ability to manage COPD patients and perform pulmonary rehabilitation.
2017 Vol. 14 (33): 74-77,85 [Abstract] ( 400 ) HTML (1 KB)  PDF (788 KB)  ( 157 )
78 Expression of DNMT1 and HMGA2 in the bladder urothelial carcinoma tissues and their clinical significance
WEI Fukui WANG Yaofeng
Objective To detect the protein expressions of the DNA methyltransferase 1 (DNMT1) and high mobility group protein A2 (HMGA2) in the bladder urothelial carcinoma (BUC) tissues and to investigate their clinical significance. Methods From January 2010 to June 2012, 89 cases of BUC cancer tissues and 45 cases of normal bladder tissues were collected from Department of Urology Surgery of Baoji Affiliated Hospital of Xi'an Medical University. The immunohistochemical SP method was used to detect the protein expression of DNMT1 and HMGA2 in each tissue. In addition, the relationship between the protein expression of DNMT1/HMGA2 and the clinical pathologic characteristics and prognosis was analyzed. Results The positive expression rate of DNMT1 in BUC cancer tissues and normal bladder tissues was 70.79% (63/89) and 6.67% (3/45), respectively (P ＜ 0.05). The positive expression rate of HMGA2 in BUC cancer tissues and the normal bladder tissues was 65.17% (58/89) and 2.22% (1/45), respectively (P ＜ 0.05). In BUC cancer tissues, the DNMT1 protein expression was correlated with the tumor diameter, lymph node metastasis status and the clinical stages (P ＜ 0.05), while the HMGA2 protein expression was correlated with the tumor diameter, differentiation grades and the clinical stages (P ＜ 0.05). The DNMT1-positive patients' survival time was much shorter than the DNMT1-negative patients (P ＜ 0.05), while the HMGA2-positive patients' survival time was much shorter than the HMGA2-negative patients (P ＜ 0.05). Conclusion In BUC cancer tissues, the DNMT1 and HMGA2 are both highly expressed, which may play critical roles in the tumor genesis and the development of BUC. DNMT1 and HMGA2 may serve as biomarkers for the prognosis and the therapy of BUC.
2017 Vol. 14 (33): 78-81 [Abstract] ( 276 ) HTML (1 KB)  PDF (422 KB)  ( 163 )
82 Effects of hemoperfusion combined with hemodialysis on cardiac valve calcification caused by maintenance hemodialysis
SONG Juxiang SONG Pei MA Hui
Objective To investigate the effects of the two methods of hemoperfusion combined with hemodialysis and hemodiafiltration for the cardiac valve calcification caused by maintenance hemodialysis. Methods Sixty patients with cardiac valve calcification underwent hemodialysis because of chronic renal failure treated in the Third Hospital of Shijiazhuang City from April 2014 to May 2016 were selected and divided into group A and B by random number table method, with 30 cases in each group. Group A was taken hemoperfusion combined with hemodialysis therapy, group B was taken hemodiafiltration, both groups were treated for 6 months. The levels of fetuin-A, high-sensitivity C-reactive protein (hs-CRP), intact parathyroid hormone (iPTH) of fasting venous blood, and the improvement of blood calcium, serum phosphate, β2-microglobulin (β2-MG) before and after treatment between the two groups were compared, and the occurrence of adverse reactions of the two groups during treatment was recorded. Results After treatment, the levels of fetuin-A in the two groups were all higher than those before treatment, which of group A was higher than that of group B, the differences were all statistically significant (P ＜ 0.05); in addition, the levels of hs-CRP, iPTH, serum phosphorus and β2-MG in the two groups were all significantly lower than those before treatment, and the indexes above in group A were all significantly lower than those of group B, the differences were all statistically significant (P ＜ 0.05); after treatment, the levels of serum calcium in the two groups were all higher than those before treatment, while group A was significantly lower than that of group B, the differences were all statistically significant (P ＜ 0.05). The total incidence of clinical adverse reactions of group A was significantly lower than that of group B, the difference was all statistically significant (P ＜ 0.05). Conclusion The effects of hemoperfusion combined with hemodialysis for preventing and controlling cardiac valve calcification caused by maintenance hemodialysis are better than that of hemodiafiltration.
2017 Vol. 14 (33): 82-85 [Abstract] ( 249 ) HTML (1 KB)  PDF (450 KB)  ( 99 )
86 Clinical observation of BuganYishen prescription in the treatment of postmenopausal osteoporosis
FU Jiangtao1 PENG Ye2 HUANG Yonghong1▲ LEI Liang1
Objective To explore the clinical effects of BuganYishen prescription in the treatment of postmenopausal osteoporosis. Methods A total of 92 cases of patients with postmenopausal osteoporosis treated in Baoji Hospital of Traditional Chinese Medicine from February 2015 to August 2016 were selected as research objects and divided into observation group and control group by random number table, with 46 cases in each group. The control group was given Alfacalcidol Soft Capsules combined with Vitamin D chewable tablets orally and the observation group was added with BuganYishen prescription on the basis of control group. The two groups were all treated for 2 courses totally, 3 months as a course. The clinical effects, the scores of visual analogue scale(VAS), bone mineral density, levels of related biochemical indexes and the incidence of adverse reactions in the two groups before and after treatment were compared. Results After treatment for 6 months, the total clinical effective rate in the observation group(95.7%) was significantly higher than that of control group(82.6%), with statistically significant difference(P ＜ 0.05). After treatment for 3 months, the VAS scores in the two groups were all lower than those of before treatment(P < 0.05), while there was no significant difference between the two groups (P ＞ 0.05). After treatment for 6 months, the VAS scores in the observation group were significantly lower than those of after treatment for 3 months and those of control group at the same stage(P ＜ 0.05). After treatment for 3 months, the bone mineral density in the two groups was all significantly higher than those before treatment(P ＜ 0.05). After treatment for 6 months, the bone mineral density in the two groups was significantly higher than those after treatment for 3 months, and that in observation group was significantly higher than that of control group (P ＜ 0.05). After treatment for 3 months, the levels of alkaline phosphatase in the two groups were lower than those of before treatment, and the levels of blood calcium, estradiol, activated Vitamin D3 were all higher than those of before treatment(P ＜ 0.05). After treatment for 6 months, the indexes above in the two groups were all better than those after treatment for 3 months, and those in the observation group was much better than those of control group(P ＜ 0.05). Conclusion BuganYishen prescription on the basis of conventional western medicine in the treatment of postmenopausal osteoporosis can obviously improve the clinical efficacy, increase bone mineral density, relieve pain and the safety is good. It is worthy of clinical promotion.
2017 Vol. 14 (33): 86-89,93 [Abstract] ( 268 ) HTML (1 KB)  PDF (577 KB)  ( 138 )
90 The effect of body mass index and age on the deep venous thrombosis of lower extremity after artificial joint replacement
Suo′nan′ang′xiu ZHOU Jingyuan QI Tengmin ZHU Hainian LIU Limin WANG Zengshun LIN Xuchen
Objective To explore the effect of body mass index (BMI) and age on the deep venous thrombosis (DVT) of lower extremity after artificial joint replacement. Methods From May 2015 to August 2016, 96 clinical data of patients underwent artificial joint replacement in Qinghai Province People′s Hospital were reviewed retrospectively and divided into DVT group (45 cases) and non-DVT group (51 cases). The proportion of BMI and age were compared between the two groups. Results A total of 45 cases of patients in artificial joint replacement occurred DVT, the incidence was 46.88%. The incidence of DVT in single hip arthroplasty was low (12.5%) and that in unilateral and bilateral knee arthroplasty were higher (65.00%, 80.66%). The proportion of non-obese patients in DVT group was lower than that of non-DVT group, and the proportion of pathological obesity, obesity and overweight were higher than those of non- DVT group, with statistically significant difference (P ＜ 0.05). The age proportion of ≤40 patients in DVT group was less than that of non-DVT group, and the proportion of ＞40-60, ＞60-70 and ＞70 were higher in DVT group than those of non-DVT group, with statistically significant difference (P ＜ 0.05). Conclusion BMI and age are the two risk factors of DVT to patients underwent artificial joint replacement. The prevention of DVT should be enhanced to prevent the occurrence of pulmonary embolism in the treatment of artificial joint replacement, especially for the obese and elderly patients.
2017 Vol. 14 (33): 90-93 [Abstract] ( 257 ) HTML (1 KB)  PDF (579 KB)  ( 83 )
94 Comparison of efficacy and hidden blood loss between anti-rotation intramedullary nail and joint replacement in the treatment of unstable intertrochanteric fractures in elderly patients
WANG Jianhua1 LIN Yan2 WU Yongdong1 GUO Pengcheng1 WANG Youbao1 LIAN Zhengang1
Objective To compare the efficacy and hidden blood loss of anti-rotation intramedullary nail and joint replacement in the treatment of unstable intertrochanteric fractures in elderly patients. Methods A total of 86 elderly patients with unstable intertrochanteric fractures in the First Hospital of Zhangjiakou from October 2014 to October 2016 were selected as the research objects and randomly divided into the observation group (43 cases) and the control group  (43 cases) by random number table. The observation group was treated with anti-rotation intramedullary nail and the control group was treated with joint replacement. The clinical parameters of the two groups (including the operation time, postoperative drainage volume and walking time under ground load), postoperative hip function, complications and hidden blood loss were compared between the two groups. Results The operation time, postoperative drainage volume and the walking time under ground load in the observation group were lower than those of control group, with statistically significant differences(P ＜ 0.05). The scores of Harris in the observation group after operation of 1, 3, 6 months were significantly higher than those of control group, with statistically significant differences (P ＜ 0.05). The incidence of postoperative complications in the observation group(6.9%, 3/43) was lower than that of control group (27.9%, 12/43), with statistically significant difference(P ＜ 0.05). The hidden blood loss and total blood loss in the observation group were significantly lower than those of control group, with statistically significant differences (P ＜ 0.05). Conclusion The curative effect of anti-rotation intramedullary nail is better than joint replacement in the treatment of unstable intertrochanteric fracture in elderly patients and has less postoperative complications. It is worthy of popularization and promotion.
2017 Vol. 14 (33): 94-97 [Abstract] ( 211 ) HTML (1 KB)  PDF (554 KB)  ( 121 )
98 Effects of minimally invasive percutaneous osteosynthesis on shoulder function of proximal humeral fractures
WANG Shouyun RUAN Wenli FENG Dianpeng
Objective To explore the effects of minimally invasive percutaneous osteosynthesis on shoulder function of proximal humeral fractures. Methods A total of 63 clinical data of patients with proximal humeral fractures treated in the Second People′s Hospital of Dalian City from June 2015 to June 2016 were reviewed retrospectively and divided into the minimally invasive group (31 cases) and the traditional invasive group (32 cases) according to the different operation methods. The minimally invasive group was treated with minimally invasive percutaneous osteosynthesis and the traditional invasive group was treated with open reduction and internal fixation. The general situation (including operation time, intraoperative blood loss, hospital stays), pain degree, the recovery of shoulder function, patients′ satisfaction and the adverse reactions were compared between the two groups. Results The operation time, intraoperative blood loss, hospital stays in the minimally invasive group were shorter or less than those of the traditional invasive group (P ＜ 0.05). After 7 d operation, the VAS score of the minimally invasive group was lower than that of traditional invasive group (P ＜ 0.05). There were no significant differences in VAS scores between the two groups in 3, 6 months after operation (P ＞ 0.05). After 15 d operation, the excellent and good rate of Neer shoulder function score of minimally invasive group was higher than that of the traditional invasive group (P ＜ 0.05). There were no significant differences in excellent and good rate of Neer shoulder function score between the two groups in 3, 6 months after operation (P ＞ 0.05). The patients′ satisfaction in the minimally invasive group was higher than that of traditional invasive group (P ＜ 0.05). Conclusion Compared with open reduction and internal fixation, minimally invasive percutaneous osteosynthesis in the treatment of proximal humeral fractures has shorter operation time and hospital stays, less intraoperative blood loss, faster shoulder function recovery, less pain and higher patients′ satisfaction.
2017 Vol. 14 (33): 98-101,105 [Abstract] ( 244 ) HTML (1 KB)  PDF (600 KB)  ( 94 )
102 The efficacy of Etoricoxib and Glucosamine Hydrochloride Capsules combined with kinesitherapy in the treatment of knee osteoarthritis
MA Chunfang YANG Qiong
Objective To explore the efficacy of Etoricoxib and Glucosamine Hydrochloride Capsules combined with kinesitherapy in the treatment of knee osteoarthritis. Methods A total of 219 clinical data of patients with knee osteoarthritis in Ningbo No.6 Hospital of Zhejiang Province from January 2016 to June 2017 were retrospectively analyzed and divided into the control group (99 cases, treated with Glucosamine Hydrochloride Capsules) and the observation group (120 cases, treated with Etoricoxib and Glucosamine Hydrochloride Capsules combined with kinesitherapy) by different treatment methods. The western Ontario and McMaster university osteoarthritis index (WOMAC), hospital of special surgery (HSS), visual analogue scale(VAS), five repetition sit-to-stand test(FTSST) and total effective rate of the two groups were compared. Results There were no statistically significant differences in the pain, stiffness, the scores of daily activity level, HSS, VAS and FTSST in the two groups before treatment (P ＞ 0.05). After treatment, the pain, stiffness, scores of daily activity level, VAS and FTSST in the two groups were lower than those of before treatment and the score of HSS was higher than that of before treatment, the indicators above in the observation group changed more significantly, with statistically significant differences (P ＜ 0.05). The total effective rate in the observation group was higher than that of control group, with statistically significant differences(P ＜ 0.05). Conclusion The clinical symptoms in the patients of knee osteoarthritis treated with Etoricoxib and Glucosamine Hydrochloride Capsules combined with kinesitherapy is improved significantly. The efficacy is good. It is worthy of promotion and application.
2017 Vol. 14 (33): 102-105 [Abstract] ( 588 ) HTML (1 KB)  PDF (554 KB)  ( 373 )
106 Therapeutic effect of Manchester surgery on patients with pelvic organ prolapse
SHEN Zhen FANG Zheng ZHOU Ying PENG Cheng WU Dabao
Objective To evaluate the therapeutic effect of Manchester surgery in patients with pelvic organ prolapse. Methods A retrospective analysis was made on 83 cases with pelvic organ prolapse who were treated with  Manchester surgery in Provincial Hospital Affiliated to Anhui Medical University from January 2009 to January 2016. The operation time, intraoperative bleeding and postoperative recovery of patients were recorded; the subjective symptoms, pelvic examination and the quality of life of patients were followed up through clinic and telephone consultation and questionnaire survey. Results 83 patients had no surgery injury or death. The average blood loss of patients of <60 years and ≥60 years [(82.31±36.91) mL vs (70.18±33.62) mL] had statistically significant difference (P < 0.05); while the mean operative time [(105.52±46.29) min vs (81.28±32.47) min], hospitalization time [(6.46±1.71) d vs (6.51±1.36) d] had no statistically significant difference (P > 0.05). The POP-Q scores postoperative showed that the symptoms of prolapse were improved significantly compared with before operation, the difference was statistically significant (P < 0.05). The pelvic floor incontinence questionnaire score and pelvic floor distress inventory score were significantly lower than those before surgery, the differences were statistically significant (P < 0.01); POP/urinary incontinence sexual questionnaire scored was lower than before surgery, with no significant difference (P > 0.05). Conclusion As a traditional surgical treatment of pelvic organ prolapse, Manchester surgery is still an effective method of treatment. Which can improve the prolapse symptoms and the quality of life.
2017 Vol. 14 (33): 106-110 [Abstract] ( 287 ) HTML (1 KB)  PDF (524 KB)  ( 117 )
111 Diagnostic value of CE-Chirp ABR in infants with suspected hearing loss and clinical application analysis
WANG Yuanming LI Chuling▲ LUO Xianglin LUO Renzhong WANG Xiaoya XU Yaxiong TAN Shujuan YIN Baozhu
Objective To explore the diagnostic value and clinical application of CE-Chirp ABR in  infants with suspected hearing loss. Methods From February 2014 to May 2015, 67 infants(143 ears)with suspected hearing loss in Dongguan Women and Children Hospital were analyzed in this study. Click (short sound stimulation) sound and CE-Chirp evoked auditory brainstem response (ABR) were performed in two groups of participants respectively and thresholds of wave V were recorded and analyzed. Results There were no significant difference in the positive rate of different degrees hearing loss or thresholds of wave V between Click sound and CE-Chirp sound (P > 0.05). Conclusion CE-Chirp ABR can be used for the evaluation of hearing loss and its value is equal to that of click ABR. However, a careful analysis and  standardization of detection method and combination with other methods should be noted in practical application.
2017 Vol. 14 (33): 111-113,125 [Abstract] ( 245 ) HTML (1 KB)  PDF (734 KB)  ( 84 )
114 Effect of dynamic-static equilibrium principle on postoperative recovery of joint function in children with supracondylar fracture of humerus
CHENG Xin ZHANG Qinfei
Objective To investigate the effect of dynamic-static equilibrium principle on postoperative recovery of joint function in children with supracondylar fracture of humerus (SFH). Methods 72 SFH children in our hospital from February 2015 to August 2016 were selected, and they were treated with open reduction and percutaneous Kirschner internal fixation. All the children were divided into two groups by random number table, 36 cases in each group. Experimental group was given functional rehabilitation based on dynamic-static equilibrium principle, while control group was given functional rehabilitation based on traditional rehabilitation principle. The rehabilitation time of fracture, flexion-extension angle of elbow joint, postoperative two-week pain degree [visual analogue scale score (VAS), the face, legs, activity, cry, consolability behavioral tool (FLACC)], postoperative twelve-week rehabilitation of elbow joint, complications in two groups were recorded. Results The rehabilitation time of fracture in experimental group was shorter than control group, while the flexion-extension angle of elbow joint was larger than control group, the differences were statistically significant (P < 0.01); the excellent rate of rehabilitation of elbow joint in experimental group was 97.22% (35/36), which was higher than 80.56% (29/36) in control group, the differences were statistically significant (P < 0.05); after surgery for two weeks, the scores of VAS, FLACC in experimental group were lower than control group, the differences were statistically significant (P < 0.01); the incidence of complications in control group was higher than that in experimental group, but there was no statistical difference (P > 0.05). Conclusion Functional exercise based on dynamic-static equilibrium principle for SFH children can shorten the rehabilitation time of fracture, promote recovery of elbow joint, enlarge the flexion-extension angle of elbow joint, decrease postoperative pain, reduce complications and have higher safety.
2017 Vol. 14 (33): 114-117 [Abstract] ( 245 ) HTML (1 KB)  PDF (509 KB)  ( 67 )
118 Analysis of the relationship between perineural invasion and clinical features of salivary adenoid cystic carcinoma
CHEN Qi
Objective To analyze the relationship between perineural invasion (PNI) and clinical features of salivary adenoid cystic carcinoma (SACC). Methods The clinical data of SACC patients treated in Stomatology Department of the Third People's Hospital of Chengdu from January 1995 to December 2015 were retrospectively analyzed. The clinical features of 54 cases potentially relevant to PNI were selected. Logistic model was used for multivariate factors analysis. Results Among 54 cases with SACC, there were 11 patients with PNI before operation, 8 cases in palatine gland, 2 cases in sublingual gland and 1 case in parotid gland. The results showed that there were no statistically significant differences in PNI among patients with different gender, ages, clinical stages, tumor sizes and lymphatic metastasis (P ＞ 0.05), but there were statistically significant differences in PNI among patients with different lesion location, pathological type and biological indicators (P ＜ 0.05). The postoperative recurrence rate of patients with PNI was higher than that of patients without PNI (P ＜ 0.05). Conversely, postoperative survival rate of patients with PNI was lower than that of patients without PNI (P ＜ 0.05). Logistic regression results showed that lesion location, pathological type and biological indicators were closely related with PNI (P ＜ 0.05). Conclusion Lesion location, pathological type and biological indicators can be used to determine whether patients have PNI or not before operation. PNI is an independent risk factor for prognosis of SACC, which can obviously increase the postoperative recurrence rate and reduce the postoperative survival rate. Preoperative examination of patients with PNI or not can be helpful for evaluation of the prognosis of the tumor.
2017 Vol. 14 (33): 118-121 [Abstract] ( 275 ) HTML (1 KB)  PDF (445 KB)  ( 127 )
122 The effect comparison between septum suture and nasal cavity filling after septum diorthosis
FU Baohua1 LIU Hong2▲ CHEN Fuquan2 ZHOU Min2
Objective To compare the effects of septum suture and nasal cavity filling after septum diorthosis. Methods The clinical data of 100 patients with perennial angiokinesis rhinitis combined with septal deviation in Department of Otorhinolaryngology Head and Neck Surgery, Xijing Hospital, Air Force Medical University from January 2016 to January 2017 were retrospectively analyzed. According to the treatment after septum diorthosis, patients were divided into septum suture group (50 cases) and nasal cavity filling group (50 cases). The VAS scores of clinical symptoms, clinical effects, nasal bleeding within 24 h after surgery, hematoma of nasal septum after surgery, ankylorrhinia of the two groups were compared. Results The VAS scores of nasal pain, headache, nasal obstruction, lacrimation, tinnitus/ear fullness, dysphagia, dyscoimesis, pain uncomfortable of clean up the nasal cavity after surgery of the nasal cavity filling group were higher than those of the septum suture group, the differences were statistically significant (P < 0.05). The total effective rate, nasal bleeding within 24 h after surgery, hematoma of nasal septum after surgery, ankylorrhinia between the two groups had no statistically significant differences (P > 0.05). Conclusion The clinical treatment effect between septum suture and nasal cavity filling after septum diorthosis has no obviously difference, but the pain of septum suture is lighter than nasal cavity filling treatment, which is worthy of clinical application.
2017 Vol. 14 (33): 122-125 [Abstract] ( 236 ) HTML (1 KB)  PDF (440 KB)  ( 99 )
126 Analysis of the community basic public health service project efficiency of Chaoyang District in Beijing
MENG Yaping1 SUN Lingli2 SONG Shuang1 WANG Shan1 WU Xin1
Objective To evaluate the efficiency of basic public health services in the communities of Chaoyang District in Beijing. Methods In 2015, 30 community health service centers in Chaoyang District of Beijing were enrolled to evaluate the efficiency of community basic public health services using DEA method. Input indicators and output indicators were designed by searching essays and Delphi method. Results The average overall efficiency, pure technical efficiency, and scale efficiency of the 30 centers were 0.838, 0.880 and 0.950. Among the 30 centers, 12 centers showed effectiveness in DEA with constant returns to scale, and 18 centers showed ineffectiveness in which 8 centers had progressively decreased scale efficiency and 10 centers had progressively increased scale efficiency. Conclusion Public health resource allocation efficiency of the 30 centers is relatively high. The effective utilization rate of public health workers is lower than that of funds. The initiative of public health personnel need to be improved. Meanwhile, it is needed to strengthen the rationality of allocation of public health funds.
2017 Vol. 14 (33): 126-129,137 [Abstract] ( 303 ) HTML (1 KB)  PDF (603 KB)  ( 115 )
130 Determination the contents of six compounds in Gansuibanxia Decoctions by high performance liquid chromatography
LI Wenjing HONG Bo LIU Jun ZHANG Jie XU Tianjiao WANG Yuchun
Objective To establish a HPLC method for simultaneous determination of six compounds (uridine, carnine, guanosine, albiflorin, liquiritin, liquiritin apioside) in Gansuibanxia Decoctions, in order to provide a theoretical basis for the quality control of Gansuibanxia Decoction. Methods The Agilent TC-C18 column (4.6 mm×250 mm×5 μm) was used at 30℃ with acetonitrile-water (gradient elution) as the mobile phase; the detective wavelength was 254 nm; the flow rate was 1.0 mL/min; the injection volume was 20 μL. Results Every standards showed a good linear relationship in the linear range, the correlation coefficients were all more than 0.9995. The recoveries were in the range of 98.1%-101.2%. RSDs were all less than 3.3%. Conclusion The method established in this paper is simple and fast with good accuracy and repeatability. It may lay the foundation for the quality control of Gansuibanxia Decoctions.
2017 Vol. 14 (33): 130-132,142 [Abstract] ( 236 ) HTML (1 KB)  PDF (883 KB)  ( 102 )
133 A Meta-analysis of the effect and safety of Lianhua Qingwen Capsules in the treatment of community acquired pneumonia
ZHOU Zhen WANG Sumei LIU Yanping
Objective To evaluate the efficacy and safety of Lianhua Qingwen Capsules in the treatment of community acquired pneumonia. Methods Medline, The Cochrane Libary, PubMed, Wanfang Database, VIP database, CNKI and CBM were searched to collect the randomized controlled trials on Lianhua Qingwen Capsule combined with conventional western medicine therapy in treating community acquired pneumonia from built library to December 2016. The studies were screened according to the inclusion and exclusion criteria, the quality of the literature was evaluated by reference to the Jadad score method, and Meta-analysis was performed by Rev Man 5.3 software. Results A total of 6 randomized controlled trials were included. The effective rate of combined treatment with Lianhua Qingwen Capsule was significantly higher than that of western medicine treatment alone (OR = 1.70, 95%CI: 1.15-2.52, P=0.008), the antipyretic time (WMD = -2.33, 95%CI: -2.52 - -2.13, P < 0.00 001), cough alleviated time (WMD = -2.63,95%CI: -2.84 - -2.41, P < 0.00001), imaging outcome time (WMD = -1.95，95%CI: -2.73 - -1.18, P < 0.00 001) were significantly shorter than those of western medicine treatment alone, the difference between the two groups were statistically significant. Two studies reported adverse reactions, and there was no statistically significant difference between the combined treatment with Lianhua Qingwen Capsules and the treatment of western medicine alone (P > 0.05). Conclusion Lianhua Qingwen Capsules combined treatment for patients with community acquired pneumonia can improve the effective rate, shorten the time of symptom relief, image outcome, less adverse reactions, and has a good adjuvant effect on community acquired pneumonia. Due to the quality and quantity of the literature, the above conclusions still need to expand the sample size, select the high quality literature for further verification.
2017 Vol. 14 (33): 133-137 [Abstract] ( 297 ) HTML (1 KB)  PDF (868 KB)  ( 120 )
138 The integrated application of School of Acupuncture and Chinese Medicine Mutual Synergizing in Lingnan in treating peripheral facial paralysis
DU Wenyi1 LI Ziping2
In long-term clinical practice, School of Acupuncture and Chinese Medicine Mutual Synergizing in Lingnan is continuous developing and innovating theory of traditional Chinese medicine (TCM) theory and therapy. Especially for the treatment of peripheral facial paralysis, It has formed a TCM treatment system which is based on the combination of acupuncture and medicine, combined with the acupuncture and cupping in Dazhui acupoint, facial flash tank, psychological counseling and personal care. In the course of treatment, the school not only pay attention to the advantage of acupuncture and point-injection, but also make a focus on the other particular therapies, and for the peripheral facial paralysis morbidity characteristic, staging treatment, individual therapy, it has achieved remarkable curative effect. This paper systematically summarizes the experience and experience of School of Acupuncture and Chinese Medicine Mutual Synergizing in Lingnan in the treatment of peripheral facial paralysis, and attached a typical medical case to discuss it together.
2017 Vol. 14 (33): 138-142 [Abstract] ( 235 ) HTML (1 KB)  PDF (726 KB)  ( 120 )
143 Analysis on medication rules of traditional Chinese medicine prescriptions for rheumatism based on data mining
ZHANG Linhua1,2 WUJiarui1 JIANG Di2
Objective To explore the medication rules of Chinese medicine in the treatment of rheumatic diseases. Methods The traditional Chinese medicine prescriptions for the treatment of rheumatism were collected and the related database was built based on Traditional Chinese Medicine Inheritance Support System (TCMISS), then the data were analyzed by Apriori algorithm and complex system entropy clustering algorithm in TCMISS to acquire the frequency of single medicine, the frequency of drug combinations, the association rules, etc. Results The highest frequency drugs included Angelicae Sinensis Radix, Glycyrrhizae Radix et Rhizoma, Paeoniae Radix Alba, Astragali Radix, Notopterygii Rhizoma et Radix, Salviae Miltiorrhizae Radix et Rhizoma, etc. The most frequent drug combinations included “Angelicae Sinensis Radix, Paeoniae Radix Alba” “Glycyrrhizae Radix et Rhizoma, Angelicae Sinensis Radix” “Chuanxiong Rhizoma, Angelicae Sinensis Radix”, etc. The association rules with confidence coefficient more than 0.7 were “CyperiRhizoma->Angelicae Sinensis Radix” “Spatholobi Caulis->Angelicae Sinensis Radix” “Chuanxiong Rhizoma->AngelicaeSinensis Radix”, etc. Conclusion The traditional Chinese medicine prescriptions for rheumatism mostly contained the drugs with the effects of benefiting Qi, nourishing blood, dispelling pathogenic wind, removing dampness, promoting blood circulation and removing blood stasis, which demonstrated the therapeutic principle of looking into both roots cause and symptoms, and treatment based on syndrome differentiation.
2017 Vol. 14 (33): 143-146 [Abstract] ( 207 ) HTML (1 KB)  PDF (787 KB)  ( 100 )
147 Study on the efficacy of external adjuvant application of Fructus Evodiae plus Natrii Sulfas on severe acute pancreatitis
LIN Lijun ZHOU Chunjiao HE Junming LIU Ming CAI Bingqin
Objective To study the influence of external adjuvant application of Fructus Evodiae plus Natrii Sulfas on the degree of systemic inflammatory response and target organ damage in patients with severe acute pancreatitis. Methods Eighty cases of patients with severe acute pancreatitis admitted to Guangdong Hospital of Traditional Chinese Medicine from February 2013 to January 2017 were collected, and the enrolled patients were divided into two groups by single blind randomized controlled method, with 40 cases in each group. The control group received conventional therapy, on basis of which, the observation group was added with external adjuvant application of Fructus Evodiae plus Natrii Sulfas. At admission, one week after treatment, the contents of serum inflammatory indexes were measured by enzyme linked immunosorbent assay (ELISA), the contents of serum liver function indexes were detected by semi automatic biochemical analyzer, the contents of intestinal mucosal barrier indexes were measured by spectrophotometer method. Results At admission, there were no significant differences in the degree of systemic inflammatory response, liver function and intestinal mucosal barrier function between the two groups (P ＞ 0.05). One week after treatment, the contents of serum interleukin-6 (IL-6), interleukin-8 (IL-6), monocyte chemoattractant protein-1 (MCP-1), alanine aminotransferase (ALT), total bilirubin (TBiL), alkaline phosphatase (AKP), lactic acid, D-amine oxidase (DAO), endotoxin of the two groups were significantly lower than those at admission, and the contents of serum IL-6, IL-8, MCP-1, ALT, TBiL, AKP, D-lactic acid, DAO, endotox of observation group were significantly lower than those of control group (P ＜ 0.05). Conclusion External adjuvant application of Fructus Evodiae plus Natrii Sulfas can alleviate the degree of systemic inflammatory response in patients with severe acute pancreatitis, and protect target organ function.
2017 Vol. 14 (33): 147-150 [Abstract] ( 280 ) HTML (1 KB)  PDF (752 KB)  ( 89 )
151 Commentary of research on constitution categories in TCM
CHEN Chao1 ZHOU Lingyun2 ZHANG Jiaqi2 WEI Hao1 YIN Junxian1 LIU Jia2 WANG Nanyue2▲
The study of traditional Chinese medicine (TCM) Constitution is one of the hot spots, however, the depth of this areais was not satisfied. There were three main problems——how to understand the concept of physical and syndromes, how to standardize identification methods and how to apply in clinical. Therefore, it is the main direction of the study of TCM Constitution to grasp the connotation of constitution, which can make physical identification method diversification, develop towards a direction of combined macro and micro, in order to achieve the combination of subjective and objective, macro and micro.
2017 Vol. 14 (33): 151-153,160 [Abstract] ( 237 ) HTML (1 KB)  PDF (720 KB)  ( 151 )
154 The clinical effect of Chaihu Guizhi Ganjiang Decoction combined with acupuncture in treating primary insomnia with syndrome of disharmony between liver and spleen
LI Shanshan1 LIU Qingquan2▲ YU Xiang1 SHI Guangxia2
Objective To explore the clinical effect of Chaihu Guizhi Ganjiang Decoction combined with acupuncture in treating primary insomnia. Methods From May 2014 to May 2016, 90 cases with primary insomnia treated in Beijing Changping Hospital of Integrated Chinese and Western Medicine were selected and divided into control group and observation group by random number table, with 45 cases in each group. The control group was treated by acupuncture therapy, and the observation group was treated by Chaihu Guizhi Ganjiang Decoction based on the control group. With 14 d as one course of treatment, the two groups were treated for 3 consecutive courses. The clinical effect, PSQI score and sleeping parameter in the two groups were compared. Results The total effective rate of the observation group was better than that of control group (P < 0.05). After treatment, the PQSI scores in the two groups were lower than those before treatment, and the PQSI score in the observation group was lower than that in the control group, with statistically significant difference (P < 0.05). After treatment, the sleeping parameter in the two groups was better than that before treatment, and the sleeping parameter in the observation group was better than that in the control group, with statistically significant difference (P < 0.05). Conclusion The clinical effect of Chaihu Guizhi Ganjiang Decoction combined with acupuncture in treating primary insomnia is remarkable, which can effectively improve sleep quality.
2017 Vol. 14 (33): 154-156,168 [Abstract] ( 254 ) HTML (1 KB)  PDF (725 KB)  ( 102 )
157 Effect of Huangdi Neijing five-note therapy combined with auricular point sticking in the treatment of hyperemesis gravidarum
HU Rong ZENG Shuxian SI Xu
Objective To explore the effect of Huangdi Neijing five-note therapy combined with auricular point sticking in the treatment of hyperemesis gravidarum. Methods The clinical data of 100 cases of early pregnant women diagnosed as hyperemesis gravidarum in Hu'nan Maternal and Child Health Hospital from January 2015 to January 2016 was analyzed retrospectively. According to different treating methods, the patients were divided into two groups. 50 cases of control group received fluid infusion of western medicine therapy, 50 cases of observation group received Huangdi Neijing five-note therapy combined with auricular point sticking. The levels of gastrin (GAS), motilin (MLT), vasoactive intestinal peptide (VIP) in the two groups were recorded before and after treatment, the required time to stop vomiting of the two groups was observed, the clinical efficacy of the two groups was evaluated, the unhealthy mentality before and after treatment in the two groups was compared by self-rating anxiety scale (SAS) and self-rating depression scale (SDS). SPSS 17.0 statistical software was used to take statistical analysis. Results After treatment, the levels of GAS, MLT, VIP in the two groups were all significantly better than those before treatment, and the levels of GAS, MLT, VIP in the observation group were significantly better than those in the control group, the differences were statistically significant (P ＜ 0.05). The required time to stop vomiting in the observation group was shorter than that in the control group, the difference was statistically significant (P ＜ 0.05). The total effective rate of observation group was 98.0%, which of control group was 88.0%, the observation group was higher than the control group, the difference was statistically significant (P ＜ 0.05). After treatment, the scores of SAS, SDS in the two groups were all lower than those before treatment, the differences were statistically significant (P ＜ 0.05), and the scores of SAS, SDS in the observation group were lower than those of control group, the differences were statistically significant (P ＜ 0.05). Conclusion Huangdi Neijing five-note therapy combined with auricular point sticking can significantly improve the unhealthy mentality of patients with hyperemesis gravidarum, and improve the clinical efficacy.
2017 Vol. 14 (33): 157-160 [Abstract] ( 309 ) HTML (1 KB)  PDF (757 KB)  ( 130 )
161 Analysis on the application of Chinese medicine injections for activating blood and resolving stasis used in hospitalized patients in one hospital from 2014 to 2016
CUI Hongjiang1 CHEN Jiaxing1 ZHANG Xiaoli1 LI Huifang2
Objective To study the use of Chinese medicine injections for activating blood and resolving stasis in one hospital for nearly three years, in order to provide reference for clinical rational drug use. Methods The number of varieties and data of the annual sales volume, the frequency of drug use, the sorting ratio and the limited daily expenses of Chinese medicine injections for activating blood and resolving stasis in the Second Affiliated Hospital of Inner Mongolia Medical University from 2014 to 2016 were collected and retrospectively analysed, and the rationality of the medicine was evaluated comprehensively. Results From 2014 to 2016, the number of varieties and annual sales of Chinese medicine injections for activating blood and resolving stasis decreased year by year. The sales volume was decreased by 8.14% and 27.64% respectively in 2015 and 2016, compared with the previous year. The annual sales amount accounted for the total sales amount 16.13%, 14.95% and 12.25%, respectively. Conclusion The medical institutions should continue to regulate the clinical application of Chinese medicine injections for activating blood and resolving stasis. Physicians should strictly follow the instructions to avoid the occurrence of related adverse reactions.
2017 Vol. 14 (33): 161-164 [Abstract] ( 273 ) HTML (1 KB)  PDF (707 KB)  ( 204 )
165 Clinical effect of Yougui Pills combined with Levothyroxine Sodium Tablets in the treatment of hypothyroidism
JIANG Min WANG Rui WANG Fen LI Shan▲
Objective To observe the clinical effect of Yougui Pills combined with Levothyroxine Sodium Tablets in the treatment of hypothyroidism. Methods One hundred cases of patients with hypothyroidism treated in the Third Affiliated Hospital of Beijing University of Chinese Medicine from June 2015 to June 2016 were selected as research objects, and they were randomly divided into control group and experimental group according to random, double blind, control principle, with 50 cases in each group. The control group received Levothyroxine Sodium Tablets, on basis of which, the experimental group received Yougui Pills. Three months later, the clinical curative effect, scores of TCM syndrome and indexes of thyroid function between the two groups were compared. Results The total effective rate of experimental group (94.0%) was significantly higher than that of the control group (80.0%), the difference was statistically significant (P ＜ 0.05). After treatment, the scores of TCM syndrome of the two groups were significantly lower than those before treatment, and the experimental group was significantly lower than that of control group, the differences were statistically significant (P ＜ 0.05). After treatment, the contents of thyroid stimulating hormone (TSH) in the two groups were significantly lower than those before treatment, and the contents of serum free triiodothyronine three (FT3) and free triiodothyronine four (FT4) were significantly higher than those before treatment, and the content of TSH in the experimental group after treatment was significantly lower than that of the control group, while the contents of FT3 and FT4 were significantly higher than those of the control group, the differences were statistically significant (P ＜ 0.05). Conclusion Yougui Pills combined with Levothyroxine Sodium Tablets in the treatment of hypothyroidism can not only increase the treatment efficiency but also improve the thyroid function of patients, which is worthy of promotion.
2017 Vol. 14 (33): 165-168 [Abstract] ( 256 ) HTML (1 KB)  PDF (750 KB)  ( 226 )
169 Exploration on anatomic approach and theory of acupotomy in the treatment of gluteus minimus myofascial pain syndrome
LIU Meiding1 LI Kaiping2 LI Dianning1
This paper reviews the literatures on gluteus minimus myofascial pain syndrome, and finds the rule that most of the trigger points of gluteus minimus are located in the middle of muscle belly, on basis of which, the anterior and posterior treatment approaches of acupotomy are designed, and all the trigger points are located accurately under relaxing posture technique, then the target treatment of acupotomy is taken. The factors influencing acupotomy efficacy, such as anatomy of gluteus minimus, complete target therapy of trigger points, recovery of trigger points, nutritional status and other factors, are analyzed, aimed at putting forward the importance of guiding acupotomy in the treatment of gluteus minimus myofascial pain by anatomical rules, so as to provide reference for acupotomy in the treatment of gluteus minimus myofascial pain in clinic.
2017 Vol. 14 (33): 169-172 [Abstract] ( 380 ) HTML (1 KB)  PDF (480 KB)  ( 200 )
173 Effect comparison of acupotomy in the treatment of cervical vertigo with simultaneous release of posterior occipital triangle and levator scapular muscle and the single release of posterior occipital triangle
GAO Xin LYU Ya'nan
Objective To compare the effect of acupotomy in the treatment of cervical vertigo with simultaneous release of posterior occipital triangle and levator scapular muscle and the single release of posterior occipital triangle. Methods From May 2015 to December 2016, 42 patients with cervical vertigo treated in Department of Tuina, the People's Hospital of Guangxi Zhuang Autonomous Region were selected and divided into two groups according to the random number table method. In the treatment group, both posterior occipital triangle and levator scapular were loosened by acupotomy at the same time. In the control group, single posterior occipital triangle was loosened by acupotomy, both groups were treated for 5 times, with each treatment interval of 2 days. Before and after treatment, the cervical vertigo symptoms and function evaluation scale of two groups were compared, and the clinical efficacy of the two groups was evaluated. Results After treatment, the scores of cervical vertigo symptoms and function evaluation scale of treatment group and control group [(24.29±3.57), (21.14±2.95) points] were significantly higher than those before treatment [(16.90±4.30), (16.76±3.73) points], and the scores of cervical vertigo symptoms and function evaluation scale of treatment group were significantly higher than those of control group, the differences were statistically significant (P ＜ 0.05). The curative effect of the two groups was evaluated, there were 15 cases of cure, 5 cases of effective, 1 case of invalid in the treatment group and 6 cases of cure, 13 cases of effective, 2 cases of invalid in the control group, the difference was statistically significant (Z = 2.62, P ＜ 0.05). Conclusion The effect of acupotomy in the treatment of cervical vertigo with simultaneous release of posterior occipital triangle and levator scapular muscle is better.
2017 Vol. 14 (33): 173-175,186 [Abstract] ( 257 ) HTML (1 KB)  PDF (847 KB)  ( 85 )
176 A case report of acute febrile neutrophilic dermatosis
OU Mei WU Hongmei LUO Yuanyuan LU Mao
The clinical manifestations of acute febrile neutrophilic dermatosis are painful red plaques or nodules, usually accompanied by fever and increased peripheral white blood cell count, percentage of neutrophils, erythrocyte sedimentation rate and C-reactive protein. Its histopathological expression is an inflammatory vascular response mainly infiltrated by neutrophils in the dermis. Acute febrile neutrophilic dermatosis is a rare skin disease that is easily misdiagnosed. To explore the clinical characteristics, differential diagnoses and treatments of acute febrile neutrophilic dermatosis, this paper reports a patient with acute febrile neutrophilic dermatosis treated in the First Affiliated Hospital of Chengdu Medical College, including his clinical and laboratory examination features and the process of diagnoses and treatments. The results indicate that to improve the definite diagnosis rate of acute febrile neutrophilic dermatosis, the clinician should attach importance to the histopathological biopsy and collect samples in multiple sites timely and repeatedly.
2017 Vol. 14 (33): 176-178 [Abstract] ( 252 ) HTML (1 KB)  PDF (457 KB)  ( 224 )
179 The application of performance appraisal in improving the quality of basic public health service
SUN Min1 JING Chunfeng2 CHEN Daozhen2
In 2009, the ministry of health issued a view on promoting the gradual equalization of basic public health services, which officially launched the equalization of public health services. Promoting the gradual equalization of basic public health services is a key part of deepening the reform of the medical and health care system in China and one of the main contents to ensure the health of the general public. Through the implementation of scientific and effective performance appraisal, it can play a powerful internal promotion and supervisory role in promoting the gradual equalization of basic public health services. Since 2016, Yangjian Health Center in Xishan District of Wuxi City break down the barriers of performance appraisal mechanism, make the concrete measures in practice, perfect the public health supervision system gradually, and then the quality of basic public health services has been improved comprehensively.
2017 Vol. 14 (33): 179-182 [Abstract] ( 259 ) HTML (1 KB)  PDF (558 KB)  ( 76 )
183 Pollution conditions and protective measures of anesthetic waste gas inhalation in Harbin operation room
LIAN Ailing1 MA Hongyan2 XING Dongmei2 YANG Fan3 WANG Dan2
Objective To investigate the pollution conditions of anestheic waste gas inhalation in Harbin operation room and develop protective measures accordingly. Methods From January to May 2015, 180 operation room in Harbin were selected from 20 hospitals by convenient sampling method and divided into inhalation group and non-inhalation group according to inhalation anesthesia. The detection rate of exhaust gas and the exhaust gas exceeding rate were compared between the two groups. The self-designed questionnaire was used to assess the congnitive of the operating room staff. Results The detection rate of exhaust gas in inhalation group (98.2%) was higher than that of non-inhalation group (72.8%), with statistically significant difference (P ＜ 0.05). The exhaust gas exceeding rate in inhalation group (58.2%) was higher than that of non-inhalation group (22.4%), with statistically significant difference (P ＜ 0.05). The proportion of choosing anesthetic exhaust gas (98.0%) as the potential risk factors was significantly higher than biological risk factor (80.0%), physical factors (56.0%) and chemical factors (73.0%) in operating room staff. Conclusion Inhalation anestheic waste gas can cause the pollution of the operation room. The staff in the operating room have higher awareness of anesthetic waste gas. It is necessary to take active and effective protective measures.
2017 Vol. 14 (33): 183-186 [Abstract] ( 263 ) HTML (1 KB)  PDF (589 KB)  ( 72 )
187 Application of virtual reality and augmented reality technology based on multimodal functional navigation in neurosurgical teaching
LI Wentao1 GONGSANG Mingjiu2 REN Chunying1▲ WEI Yan1 MENG Xijun1
Neuroanatomy is complicated and difficult to master. The operation is difficult and it has higher requirements for minimally invasive and meticulous level. In addition, the teaching, training and dissection methods of department of neurosurgery operation are limited. At present, the application of virtual reality technology in department of neurosurgery teaching and clinical practice has been reported, but there are few reports about the application of augmented reality technology in neurosurgical teaching. This paper introduces the multimodal neuronavigation planning platform, virtual reality and augmented reality technology in neurosurgical teaching are analyzed and discussed.
2017 Vol. 14 (33): 187-189 [Abstract] ( 257 ) HTML (1 KB)  PDF (544 KB)  ( 103 )
190 Study on the cultivation of socialist core values of college students from the perspective of"drug morality"
TAO Hui
To implement the "healthy China" strategy, the safety and regulatory level of food and drug production need to be improved and a contingent of highly qualified medical talents with both ability and political integrity needs to be built. Medical colleges shoulder the important task of cultivating national medical talents and should adhere to the fundamental moral education, promote the development of pharmaceutical industry, ensure the drug safety. The socialist core values guide "drug morality" education and integrate it into students' life, work and spiritual world. From the fusion perspective of medical ethics and socialist core values, this article puts forward four elements innovating cultivation path such as designing special activities, playing a professional background, integrating industry resources and relying on the information technology, thereby improving the educational effects of socialist core values.
2017 Vol. 14 (33): 190-193 [Abstract] ( 255 ) HTML (1 KB)  PDF (703 KB)  ( 105 )
Copyright © Editorial Board of 中国医药导报
Supported by:Beijing Magtech