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| Study on the mechanism of Metformin in the treatment of polycystic ovary syndrome |
| LU Chenyu QIN Peigang DU Hanyu WU Haixia Aihemaiti·Guliyaer AI Lixia XU Qi |
| Basic Medical College, Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830011, China |
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Abstract Objective To investigate the therapeutic mechanism of Metformin on Treg cells and Th17 cells in polycystic ovary syndrome (PCOS). Methods 45 female Wister rats were gavaged by Letrozole in order to develop PCOS, and randomly chosen 5 rats to confirm the establishment of model. Then rats were divided into Metformin group [300 mg/(kg·d)], Diane group [180 mg/(kg·d)], Metformin [300 mg/(kg·d)] combined with Diane [180mg/(kg·d)] group and model group, randomly, each group had 10 rats. The other 10 female rats were arranged as control group. PCOS rats were given corresponding drugs, while control group were administrated with physiological saline, the treatment period of all groups were 28 days. The weight changes of rats weekly were observed. Ovarian histological changes were examined by Yihong-hematoxylin staining (HE). Flow cytometry was applied to delineate the phenotype of Treg cells and Th17 cells in spleen. ELISA was used to measure IL-6 and TGF-β in serum. The expression levels of Foxp3 and ROR-γt were measured by RT-PCR. Results The weight, the Treg and Th17 in spleen, Treg/Th17 ratio, the levels of IL-6 and TGF-β in serum, the expression of Foxp3 of PCOS model group were higher than control, with statistically significant difference (P < 0.05). The weight of Metformin group, Diane group and combined group were similar to control group (P > 0.05), but significantly lower than model group (P ＜ 0.05). The cystic dilated follicle in Metformin group, Diane group and combined group decreased significantly compared with the model group. Treg cells and Th17 cells in spleen, IL-6 and transcription factor ROR-γt in serum of the Metformin group were significantly higher than the model group (P ＜ 0.05). Th17 cells in spleen and IL-6 in serum of Diane group and the combined group were significantly lower than the model group (P ＜ 0.05), but the expressions of transcription factor Foxp3 in ovarian tissue were significantly higher than the model group (P ＜ 0.05). Conclusion Metformin treatment of PCOS may affect the number and proportion of Treg and Th17 cells by affecting the expression level of the serum IL-6, which can improve the microenvironment of ovarian tissue.
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