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| A preliminary study on the cross blood-brain-barrier transport of exosomal microRNA-135a |
| LIU Chengeng1 HAO Ting2 YANG Tingting1 MENG Shuang3 WANG Peichang1 |
1.Department of Clinical Laboratory, Xuanwu Hospital, Capital Medcial University, Beijing 100053, China;
2.Department of Pathology, Heze Municipal Hospital, Shandong Province, Heze 274000, China;
3.State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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Abstract Objective To study the effect of microRNA-135a (miR-135a) on blood-brain barrier and cell transport in exocrine. Methods The high miR-135a exosomes were extracted from the cerebrospinal fluid (CSF) of the transgenic mice and injected into the ventricles of the wild type mice and intervened with SH-SY5Y cells. The levels of miR-135a in the peripheral blood of mice and the exosomes of the culture, and the expression and activity of β-secretase-1 (BACE-1) in SH-SY5Y cells were determined. Results Intraventricular injection of miR-135a exosomes resulted in a significant increase in CSF and plasma exosomal miR-135a in wild-type mice (P < 0.05). The activity of BACE-1 in SH-SY5Y cells treated with exosomes harvest from the CSF of APP/PS1 double transgenic mice was significantly lower than that in other intervention group and control group (P < 0.05), and the mRNA expression level of SH-SY5Y cells was significantly lower than that of control (P < 0.05). The trend of change is consistent with the trend of activity. Conclusion The signal of "high miR-135a" can be transferred from CSF to blood through the blood-brain barrier, which provides a more solid experimental basis for the use of exosomal miR-135a as a biomarker of Alzheimer's disease.
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