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2018 Vol. 15, No. 13
Published: 2018-05-05
 
 
4 Expression and clinical significance of IMP3 and CD164 in glioblastomas
WU Jing1,2 WANG Jingjing2 LI Xiaojie2 HE Jie2,3
Objective To investigate the expression and clinical significance of the oncogene IMP3 and sialomucin CD164 in glioblastoma (GBM) tissues. Methods One hundred and nineteen GBM paraffin-embedded samples from January 2011 to July 2015 in Anhui Provincial Hospital were collected. The expression of IMP3 and CD164 was detected by automated immunohistochemistry. The results were analyzed with clinical and pathological factors and the survival of patients. Results The high expression rates of IMP3 and CD164 were 69.7% (83/119) and 81.5% (97/119) respectively. There was a positive correlation between the two expression levels (r = 0.252, P = 0.006). In the connected analysis with clinical data, the expression of IMP3 was positively correlated with EGFR state (r = 0.242, P = 0.008), and was not significantly related to the gender, age, tumor size, tumor location and Ki67 proliferation index (P ＞ 0.05). CD164 expression was positively correlated with age (r = 0.285，P = 0.002) and EGFR (r = 0.249，P = 0.006), not other factors (P ＞ 0.05). The overall survival (OS) of patients with CD164 high expression were significantly shorter than those with low expression (P = 0.004). There was no significant correlation between IMP3 and OS alone (P = 0.056). Age and CD164 were independent prognostic factors of GBM (P = 0.036, 0.005). Conclusion The expression of CD164 in GBM is closely related to IMP3, and CD164 can be used as an independent predictor of poor prognosis of GBM.
2018 Vol. 15 (13): 4-8 [Abstract] ( 240 ) HTML (1 KB)  PDF (603 KB)  ( 242 )
9 The effects of non-myeloablative conditioning and cord blood hematopoietic stem cells transplantation on spermatogenic function in mice
ZHANG Meng1* YAO Guanping2* HE Fang1 LI Yuxiang3 FAN Zhenhai1 YU Limei1
Objective To explore the effects of chemotherapeutic drugs non-myeloablative pretreatment and human cord blood hematopoietic stem cells transplantation (HSCT) on the spermatogenic function in mice. Methods Thirty Balb/c mice were divided into 3 groups by random digital table method, with 10 mice in each group, which contained control group, Busulfan (BU) combined with Cyclophosphamide (CY) non-myeloablative conditioning (BU+CY) group and HSCT group. In addition to the control group, BU+CY group and HSCT group were pretreated with BU 10 mg/kg and CY 120 mg/kg to induce testicular lesion, and HSCT group was taken human umbilical cord blood HSCT. After HSCT for three weeks, the testicular index was calculated by weighting method. The routine quality analysis of semen and histopathological examination of the testis and epididymis were carried out. Results Compared with the control group, the testicular index, sperm density and sperm viability in BU+CY group were significantly decreased, but the sperm malformation rate was significantly increased (P ＜ 0.01). The graft versus host disease (GVHD) score was higher in HSCT group. The testicular index, sperm density and sperm viability in HSCT group were higher than those in BU+CY group (P ＜ 0.05), while the sperm malformation rate was lower than that of BU+CY group (P ＜ 0.01). The pathological examination found that the testicular volume in BU+CY group was smaller, the seminiferous tubules were obviously impaired, the spermatogenic cells were swelled and arranged in disorder, the seminiferous epithelia was thinned, the number of sperm was significantly reduced in seminiferous tubule and epididymal tubule. Compared with the BU+CY group, the testicular volume in HSCT group was increased, the seminiferous tubule was more integrity, and the number of sperm was increased, but the number of layers seminiferous epithelia cells and spermatocyte were still less. Conclusion BU+CY non-myeloablative conditioning can cause obvious testis injury. Cord blood HSCT can promote a certain extent recovery of testicular spermatogenesis in mild acute GVDH mice.
2018 Vol. 15 (13): 9-13 [Abstract] ( 235 ) HTML (1 KB)  PDF (1302 KB)  ( 110 )
14 Experimental study on culturing Schwann cells of neonatal rats in vitro
ZHOU Min HU Ming HE Songming HUANG Guotao CHEN Qian HONG Li
Objective To explore a culture of SCs method with higher cell purity and shorter time by comparing the two methods including the double-enzyme digestion and single-enzyme digestion of primary culture of Schwann cells (SCs) using the neonatal rats. Methods Bilaterally sciatic nerves were dissected from 4 to 5-day-old neonatal SD rats and double-enzyme digestion method and single-enzyme digestion method which both combined with hemi-explants-culture method, differential digestion method, differential adhesion method, and cytosine arabinoside were used to culture and purify SCs, respectively. The morphology of the SCs was observed under the inverted microscope at 48 hours and 6 days of culture. Then the SCs were digested and passaged after 7 days in culture and counted under the cell counter. The purity of SCs was identified by S-100 immunofluorescence staining. CCK-8 was used to measure the P3 cells growth curve, respectively. Results With the same number of neonatal rats, double-enzyme digestion method acquired SCs at least twice than single-enzyme digestion method. (95.29±1.71)% of S-100 positive SCs cultured were shown by immunofluorescence staining in the double-enzyme digestion method group, and (72.41±2.16)% in the single-enzyme digestion method group (P < 0.05). There was no difference between the two cultures in the growth curve of P3 cells (P > 0.05). Conclusion The double-enzyme digestion combined with hemi-explants-culture method, differential digestion method, differential adhesion method, and cytosine arabinoside may obtain sufficient amount of high-purity SCs in a short time and be applied in the further cytological experimental research on peripheral nerve regeneration.
2018 Vol. 15 (13): 14-17 [Abstract] ( 431 ) HTML (1 KB)  PDF (838 KB)  ( 73 )
18 Effect of macrophages and pulmonary microvascular endothelial cells on Aspergillus fumigatus
SONG Jun HAN Jing SUN Yue SHI Weimin▲
Objective To investigate effect of macrophages and pulmonary microvascular endothelial cells (HPMVECs) on Aspergillus fumigatus. Methods In vitro models of Aspergillus fumigatus-treated HPMVECs and Aspergillus fumigatus-treated J774 cells were established, confocal laser scanning microscope was used for detection of co-cultures. Results Aspergillus fumigatus were phagocyted by J774 cells. However, Aspergillus fumigatus were not internalized by HPMVECs, onlu morphology change and integrity compromise were observed on Aspergillus fumigatus-treated HPMVECs. Conclusion J774 cells phagocytes Aspergillus fumigatus. HPMVECs can't internalize Aspergillus fumigatus. Aspergillus fumigatus can cause morphology change and integrity compromise in HPMVECs.
2018 Vol. 15 (13): 18-20 [Abstract] ( 265 ) HTML (1 KB)  PDF (898 KB)  ( 438 )
21 Study on SPTLC2 expression level in brain tissue in the rat model of Parkinson disease
HE Xiaoyan JIAO Yan LI Hongyan
Objective To explore the expression of serine palmitate transferase long chain subunit 2 (SPTLC2) in brain tissue in the rat model of Parkinson disease. Methods Parkinson rat model was prepared by injecting 6-hydroxy dopamine, and the rats were divided into three groups, 2-week model group, 4-week model group, 8-week model group, and normal group. Behaviors of rats in each group were assessed by using abnormal involuntary movement (AIM) scoring method. Rotation duration, starting time, the cycle number of agent peak were recorded meanwhile. HE staining method was used to observe the changes of brain tissue in rats, and the expression of α synuclein in rat brain tissue was detected by immunohistochemistry, and SPTLC2 and mRNA expression in brain tissue of rats was detected by using RT-PCR, and SPTLC2 protein expression in rat brain tissue was detected by Western blot. Results AIM score, rotation duration, starting time, agent peak rotation number in model group was higher than those in the normal group, and with the extension of time, AIM score, rotating start time, agent peak rotation number gradually increased, while the rotation starting time gradually decreased, with statistically significant differences (P ＜ 0.05). Immunohistochemical staining showed that the number of positive cells in the normal group was less than that in the normal group, and the number of positive cells in the model group increased significantly. The number of positive cells increased gradually with the extension of time. Compared with those in the normal group and 2-week model group, SPTLC2 gene and protein expression in 4-week and 8-week model group increased, and compared with 4-week model group, SPTLC2 gene and protein expression in 8-week model group increased, with statistically significant differences (P ＜ 0.05). Conclusion SPTLC2 may be involved in the pathogenesis of Parkinson disease, via up-regulating SPTLC2 gene and protein expression.
2018 Vol. 15 (13): 21-24,30 [Abstract] ( 244 ) HTML (1 KB)  PDF (704 KB)  ( 206 )
25 Expression status and prognostic significance of lncRNA H19 in thyroid carcinoma
LIU Na1 QI Yanhua1 ZHOU Qi1 CHENG Chuantao2 YANG Wenbin2
Objective To explore the expression of long non-coding RNA H19 (lncRNA H19) in thyroid carcinoma (TC), and its correlation with the clinicopathologic characteristics and prognosis of TC. Methods From January 2009 to January 2012, 131 TC tissues and adjacent tissues were collected from patients after thyroid surgery between as case group. Concurrently, additional 122 normal thyroid tissues from patients with benign thyroid lesions were obtained as normal control group. The expression level of lncRNA H19 were detected by quantitative real-time PCR (qRT-PCR). five years follow-ups were conducted. Receiver operating curve(ROC) was used to evaluate the diagnostic values of lncRNA H19 for TC. The Kaplan-Meier method were employed to analyze 5-year survival rate of TC patient. Both univariate and multivariate analyses were performed to analyze the prognostic factors of TC. Results The lncRNA H19 expression was higher in TC tissues than that in the adjacent and normal tissues(P < 0.05). The area under the ROC curve(AUC) of lncRNA H19 were 0.801. Diagnostic boundary point was 3.58. Sensitivity and specificity was 72.5% and 75.4% respectively. Youden index was 0.479. The lncRNA H19 expression in TC tissue with tumor diameter no less than 1 cm, TNM Ⅲ+Ⅳ stage, or lymph node metastasis was evidently higher than that in TC tissue with tumor smaller than 1 cm, TNM Ⅰ+Ⅱ stage, or without lymph node metastasis(P < 0.05). The 5-year survival rate of TC patients with high lncRNA H19 expression declined (28.44%). The expression of  lncRNA H19, tumor diameter, TNM stage and lymph node metastasis were independent prognostic factors of TC patients (P < 0.05). Conclusion The increased lncRNA H19 expression levels are associated with clinicopathologic characteristics and poor prognosis in TC patients. And it may be one of the molecular regulation mechanism in TC occurrence and development. This study also can provide basis for noninvasive diagnosis and therapeutic target searching in TC.
2018 Vol. 15 (13): 25-30 [Abstract] ( 194 ) HTML (1 KB)  PDF (711 KB)  ( 76 )
31 Study on the effect of Magnoliae Officinalis Cortex on gastrointestinal motility disorders
CHAO Lei1 CAO Yudan2 CHEN Peidong2 ZHANG Li2
Objective To investigate the mechanism of Magnoliae Officinalis Cortex on promoting gastrointestinal motility of mice. Methods Three hundred and four male ICR mice were randomly divided into low, medium, high dose of Magnoliae Officinalis Cortex extract groups (1, 2, 4 g/kg), low, medium, high dose of magnolol groups (40, 80, 160 mg/kg), positive control group (Etobil Hydrochloride, 50 mg/kg) and blank control group according to the body weight, with 8 mice in each group. The intestinal propulsive rate of normal mice, and the gastric empty and intestinal propulsive rate of Atropine (1 mg/kg) and Ondansetron (1.6 mg/kg) induced mice were measured respectively. The multi-channel physiological instrument was used to detect the muscle electrophysiological waveform. Results Compared with blank control group, the intestinal propulsive rates of normal mice in medium, high dose of Magnoliae Officinalis Cortex extract groups and medium, high dose of magnolol groups were significantly improved (P ＜ 0.05). Compared with Atropine model group, the gastric emptying rates in medium, high dose of Magnoliae Officinalis Cortex extract groups and low, medium, high dose of magnolol groups were significantly improved (P ＜ 0.05 or P ＜ 0.01). Compared with Atropine model group, the intestinal propulsive rates in high dose of Magnoliae Officinalis Cortex extract group and high dose of magnolol group were improved, the differences were statistically significant (P ＜ 0.05). Compared with Ondansetron model group, the gastric emptying rate and intestinal propulsive rate in high dose of magnolol group were improved, the differences were statistically significant (P ＜ 0.05). Conclusion Magnoliae Officinalis Cortex extract and magnolol can improve the gastric emptying rate and intestinal propulsive rate of mice, the positive effect for gastrointestinal motility may be connected with 5-HT3 receptor and M acetylcholine receptor.
2018 Vol. 15 (13): 31-34 [Abstract] ( 331 ) HTML (1 KB)  PDF (535 KB)  ( 78 )
35 Advanced in the research of miR-375 on tumor development and diagnosis
SUI Chengliang1 HUANG Zhaoquan2
MicroRNAs (miRNAs) are conserved small non-coding RNAs that play important roles in various biological processes via controlling gene expression. Among them, miR-375 is firstly identified in pancreatic β-cells and proved to regulate insulin secretion and islet formation. Furthermore, it turns that miR-375 is involved in cancer development and progression by targeting several important oncogenes including AEG-1, YAP1, IGF1R and PDK1. Unlike some other cancers, the levels of miR-375 in prostate and breast cancer specifically increase, indicating its action in tumor development. Therefore, miR-375 may be a potential target for suppressing tumor growth, and has the clinical value as a biomarker for early diagnosis and prognosis prediction.
2018 Vol. 15 (13): 35-37,46 [Abstract] ( 260 ) HTML (1 KB)  PDF (833 KB)  ( 162 )
38 Research progress of health management for PCI postoperative patients
FU Guangfang1 WU Min2 SUN Jing3▲
Cardiovascular diseases, especially coronary heart disease, seriously threaten human health. With the development of medical science, PCI has become an important method for the revascularization of coronary heart disease and a more effective measure for patients with acute coronary syndrome (ACS). PCI has many advantages, such as less trauma and obvious relief in symptoms. However, postoperative risk of coronary artery restenosis, stent thrombosis, and recurrence of adverse cardiac events were still present. The significance, main content and commonly used methods of health management for PCI postoperative patients are introduced in this review. It also emphasizes the application of the internet-based new health management strategies for PCI postoperative patients. The paper is aimed at providing the nursing staff more references to further explore and improve the existing health management model for PCI postoperative nursing patients.
2018 Vol. 15 (13): 38-41 [Abstract] ( 189 ) HTML (1 KB)  PDF (407 KB)  ( 370 )
42 Study of cognitive function in ultra-high-risk psychosis population and first episode schizophrenia
ZHANG Rongrong YAN Wei LV Lanlan XIE Shiping▲
Objective To evaluate the cognitive function of patients with the first episode schizophrenia and Ultra-high-risk for psychosis and figure out the cognitive function of schizophrenia in different periods and related factors. Methods Twenty-eight patients with first episode schizophrenia (first episode group) and 24 patients with ultra-high-risk for psychosis (UHR group) were treated in Affiliated Nanjing Brain Hospital of Nanjing Medical University from May 2017 to February 2018 were selected, and 38 well-matched healthy controls (control group) were assessed with the Wechsler Adult Intelligence Scale for intelligence and MATRICS Consensus Cognitive Battery (MCCB) for cognitive function, the differences of cognitive function among the three groups were compared, while the correlation analysis was carried out. Results The difference of the scores of IQ, speed of processing, verbal learning, working memory, reasoning, visual learning, social cognition and attention/vigilance in three groups was significant (P < 0.05). Compared with the control group, both the first episode group and UHR group showed worse performance in IQ, speed of processing, verbal learning, working memory, reasoning, visual learning, social cognition and attention/vigilance (P < 0.05), compared with the UHR group, first episode group showed worse performance in speed of processing, verbal learning, working memory, visual learning, social cognition (P < 0.05). The positive symptoms of UHR group were negatively correlated with the scores of TMT and NAB, the negative symptoms were negatively correlated with the scores of TMT, BACS, Fluency, WMS, NAB, BVMT-R, CPT-IP, (P < 0.05) IQ were positively correlated with the scores of TMT, BACS, Fluency, HVLT-R, NAB, CPT-IP (P < 0.05). Conclusion Widespread cognitive impairments exist in ultrahigh-risk psychosis and the impairments may start before the onset of the disease, and cognitive function will aggravate with the progression of the disease.
2018 Vol. 15 (13): 42-46 [Abstract] ( 755 ) HTML (1 KB)  PDF (662 KB)  ( 127 )
47 The expression and influencing factors of AIRE in peripheral blood of myasthenia gravis
WANG Shengyuan1* XIAO Fenglin2* LI Hang1 ZHANG Yu1
Objective To study the expression and influencing factors of AIRE in peripheral blood of MG. Methods Clinical data and peripheral blood samples of MG patients were collected at Tangdu Hospital of air force Military Medical University from January 2016 to January 2017. All data were divided into general type MG (GMG) group (16 cases), ocular MG (OMG) group (14 cases) and control group (10 cases). Flow cytometry was used to analyze the ratio of AIRE positive cells, Tfr cells and Tfh cells. Results The express of AIRE in GMG group was significantly lower than that in OMG group and control group (P=0.003), but there was no significant difference between OMG group and control group (P=0.152). The express of AIRE was positively correlated with the ratio of Tfr/Tfh (r=0.605, P ＜ 0.001), but negatively correlated with the QMG score and clinical absolute score (r=-0.622, P ＜ 0.001; r=-0.513, P=0.004). Conclusion AIRE may be involved in the pathogenesis of MG, and low level of AIRE may play a key role in the pathogenesis of MG.
2018 Vol. 15 (13): 47-50 [Abstract] ( 207 ) HTML (1 KB)  PDF (856 KB)  ( 98 )
51 Rehabilitation efficacy of exercise rehabilitation training combined with ultrashort wave and dynamic interference on periarthritis of high level athletes
LIU Ming1 LU Hongyuan2
Objective To explore the rehabilitation efficacy of continuous passive motion combined with ultrashort wave and dynamic interference in the treatment of periarthritis of the high level athletes. Methods From May 2016 to May 2017, 150 cases of periarthritis of shoulder athletes in Xi′an Institute of Physical Education Sports Injury Rehabilitation Hospital and Rehabilitation Hospital of Shaanxi Province were selected, according to the research needs to randomly set athletes as experimental group (n=50) and control group 1 (n=50) and the control group 2 (n=50). The experimental group, the control group 1 and the control group 2 athletes were treated with continuous passive motion combined with ultrashort wave and dynamic interference treatment, continuous passive motion therapy and ultrashort wave and dynamic interference treatment respectively. Results athletes in The total efficiency of experimental group was 98.0%, significantly higher than that in the control group 1 (74.0%) and control group 2 (78.0%), the differences were statistically significant (P < 0.05). The VAS score and Melle score of two groups were compared before treatment, the difference was not statistically significant (P < 0.05). 1, 3 months and 5 months of treatment, VAS score and Melle score were significantly lower than before treatment, the differences were statistically significant (P < 0.05). However, the VAS scores and Melle scores of athletes in the experimental group at 1, 3 months and 5 months of treatment were significantly lower than those in the control group 1 and the control group 2, and the differences were statistically significant (P < 0.05). However, the VAS scores and Melle scores of the control group 1 and the control group 2 at 1, 3  months and 5 months of the treatment were not statistical significant (P < 0.05). Conclusion Continuous passive motion combined with ultrashort wave and dynamic interference current therapy for high level athletes can significantly improve the pain degree of scapulohumeral periarthritis of athletes, improve the shoulder joint function recovery effect, clinical treatment effect has been improved , so as to promote the periarthritis of shoulder.
2018 Vol. 15 (13): 51-54 [Abstract] ( 200 ) HTML (1 KB)  PDF (647 KB)  ( 105 )
55 Research on leptin in the pathogenesis of obese adult asthma
LI Jun WENG Yuqing ZENG Liheng ZENG Fanrui
Objective To observe the correlationship between serum leptin, Th1/Th2 cytokine and symptom severity in obese adult asthma patients, and investigate the pathogenesis of  obese adult asthma. Methods From July to December 2016, 100 patients with asthma admitted to the Zhuhai People′s Hospital (“our hospital” for short) were selected as research objects, and they were divided into obese asthma group (50 cases) and non-obese asthma group (50 cases)by BMI. Fifty cases of healthy people at the same time in our hospital for physical examination were selected as control group, and were divided into obese group (25 cases) and non-obese control group (25 cases). Serum leptin, INF-γ and IL-4 were detected using ELISA, as well as did the concurrent pulmonary function and asthma contml test (ACT). The correlationship between serum leptin, INF-γ, IL-4, pulmonary function and ACT was analyzed. Results The serum leptin, IFN-γ and IFN-γ/IL-4 in obese asthma groud were significantly higher than those in other groups (P < 0.05). The leptin levels in obese asthma groud showed a negative correlation with ACT and FEV1%Pred (r= -0.501, -0.384, all P < 0.05), but a positive correlation with IFN-γ and IFN-γ/IL-4 (r= 0.446, 0.423, all P < 0.05), and no correlation with IL-4 (r= -0.189, P ＞ 0.05). Conclusion The serum leptin in obese asthma patients has a higher level and a positive correlation with clinical severity, and probably plays a role in airway hyperresponsiveness by Th1 cytokine mediating.
2018 Vol. 15 (13): 55-58 [Abstract] ( 225 ) HTML (1 KB)  PDF (767 KB)  ( 379 )
59 Clinical study on skin distractor combined with negative pressure wound therapy in treating large area cutaneous defect wound
ZHU Hongwei1 YAN Mengyang1 ZHANG Yulong2 YU Yonglin3 SHENG Quanfeng1
Objective To discuss the clinical effect of skin distractor combined with negative pressure wound therapy (NPWT) in the treatment of large area cutaneous defect wound (LACDW). Methods From January 2015 to June 2017, in Second Hospital of Qinhuangdao City, 61 patients with LACDW were selected, they were divided into observation group (31 cases) and control group (30 cases) according to the random number table. The control group was treated with NPWT technique, the observation group was treated with skin distractor on the basis of control group. The wound area, hyaluronic acid (HA) and vascular endothelial growth factor (VEGF), bacterial count of wound secretions in two groups were compared, the correlations of HA and VEGF levels with wound area and bacterial count of wound secretions were analyzed. Results The wound area and the bacterial count of the wound secretions in the two groups were gradually decreased after treatment, the wound area and the bacterial count of wound secretions on the 1st, 7th and 10th day after treatment in observation group was significantly smaller than the control group, the differences were statistically significant (P < 0.05). On the 10th day after treatment, the levels of HA and VEGF in the two groups were significantly higher than before treatment, the levels of HA and VEGF in observation group were significantly higher than the control group, the differences were statistically significant (P < 0.05). The result of Spearman correlation analysis method showed that the level of HA was negatively correlated with the wound area and the bacterial count of wound secretions (r = -0.688, -0.579, P < 0.05). The level of VEGF was negatively correlated with the wound area and the bacterial count of wound secretions (r =-0.623, -0.594, P < 0.05). Conclusion The clinical effect of skin distractor combined with NPWT in the treatment of LACDW is effective, it can effectively reduce the area of defect wound and bacteria count of wound secretions, and increase the levels of VEGF and HA, it is worth to be promoted.
2018 Vol. 15 (13): 59-62,71 [Abstract] ( 249 ) HTML (1 KB)  PDF (673 KB)  ( 78 )
63 The analysis of the expression of miR-200a and miR-92a and the relation of clinicopathological features in colorectal cancer
YANG Xinhui ZHAO Weimin DAReBai·Redati ZENG Xiangyue
Objective To analyze the expression of miR-200a and miR-92a in colorectal cancer and the relation of clinicopathological features. Methods The 60 patients with colorectal cancers in Affiliated Tumor Hospital, Xinjiang Medical University for surgical treatment from February 2011 to August 2012 were collected. And the 60 colorectal cancer tissue specimens and paracancerous tissues were achieved in patients with colorectal cancer respectively. The positive expression rates of miR-200a in colorectal cancer tissues and paracancerous tissues were detected by in situ hybridization technique. The expression levels of miR-92a in colorectal cancer tissues and paracancerous tissues were detected by real-time PCR. The relativities of the expression of miR-92a and miR-200a and the clinicopathological features were analyzed. Follow-up 5 years, all patients with colorectal cancers were divided into survival group and death group according to the survival status of patients. The expression levels of miR-92a and miR-200a were compared between two groups. Results The positive rate of miR-200a and the expression level of miR-92a in colorectal cancer tissues were significantly higher than in paracancerous tissues (all P ＜ 0.05). The positive expression rates of miR-200a in the colorectal cancer patients of the middle and high degree of differentiation and TNM stage Ⅰ-Ⅱ were significantly lower than those of the low degree of differentiation and TNM stage Ⅲ-Ⅳ, which were 40.00%(18/45) vs. 73.33%(11/15), 58.06%(18/31) vs. 86.21%(25/29) (all P ＜ 0.05). The relative expressions of miR-92a in colorectal cancer patients of the TNM stage Ⅰ-Ⅱ and without lymph node metastasis were significantly lower than those of the TNM stage Ⅲ-Ⅳ and lymph node metastasis, the differences were statistically significant (all P ＜ 0.05). Follow-up 5 years, the survival group had 21 cases and death group had 39 cases. The positive rate of miR-200a and the relative expression of miR-92a in survival group were significantly lower than death group, the differences were statistically significant (all P ＜ 0.05). Conclusion The expression levels of miR-200a and miR-92a are highly in colorectal cancer, and are closely associated with the severity and prognosis.
2018 Vol. 15 (13): 63-66,75 [Abstract] ( 296 ) HTML (1 KB)  PDF (822 KB)  ( 70 )
67 Study on the effects of Somatostatin, Pituitrin and Omeprazole triple therapy in the treatment of cirrhosis with hemorrhage of gastroesophageal varices in serum GAS, GLC and hemodynamics
LI Chao
Objective To investigate the effects of Somatostatin, Pituitrin and Omeprazole triple therapy in the treatment of cirrhosis with hemorrhage of gastroesophageal varices in serum GAS, GLC and hemodynamics. Methods From December 1, 2012 to June 5, 2016, 117 cases of cirrhosis with hemorrhage of gastroesophageal varices were collected in the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, according to the type of treatment, were randomly divided into Somatostatin group, Pituitrin group and combined group, with 39 cases in each group, Somatostatin group was treated with Somatostatin+Omeprazole, Pituitrin group were treated with Pituitrin+Omeprazole and the combined group were treated with Somatostatin+Pituitrin+Omeprazole, and three groups were treated for 24 to 48 h. The pressure, portal venous pressure, portal vein diameter, portal vein blood flow velocity of central vein were measured, the determination of GAS and GLC in blood samples were collected and clinical effect, dynamic changes, adverse events were compared after treatment. Results Compared with that before treatment, PVP, DPV value, GAS, and GLC of the three groups after treatment decreased, the differences were statistically significant （P < 0.05). Compared with Pituitrin group and Somatostatin group, levels of PVP and DPV were lower, GAS and GLC were lower, the differences were statistically significant （P < 0.05). The improved rate of the Pituitrin group （74.36%) and Somatostatin group （71.79%) were lower than the combined group （92.31%), the difference was statistically significant （P < 0.05). The hemostatic time, average blood transfusion and hospitalization time of the Pituitrin group and the Somatostatin group were higher than  the combined group （P < 0.05). Conclusion Somatostatin, Pituitrin and Omeprazole triple therapy can improve the liver cirrhosis with esophageal and gastric variceal bleeding bleeding, reduce portal vein pressure, gastrointestinal hormone, is a kind of effective treatment.
2018 Vol. 15 (13): 67-71 [Abstract] ( 235 ) HTML (1 KB)  PDF (709 KB)  ( 137 )
72 Effect of synchronized intermittent mandatory ventilation on blood gas indexes in neonates with respiratory failure
YAO Heping XU Peilin Wang Yingying
Objective To investigate the effect of synchronized intermittent mandatory ventilation (SIMV) on blood gas indexes in neonates with respiratory failure. Methods From June 2015 to June 2017, in NICU of Shaoxing Central Hospital, 116 neonates with respiratory failure were selected, according to the choice of ventilation mode, they were divided into SIMV group (60 cases, given SIMV ventilation) and continuous positive airway pressure (CPAP) group (56 cases, given CPAP). The clinical curative effect, blood gas indexes (PaCO2, PaO2, SpO2, OI) 3 d after treatment, the proportion of intubation and ventilator setting, duration of ventilation, hospital stay and incidence of complications in the two groups were compared. Results The effective rate in SIMV group was 93.33%, that in CPAP group was 80.36%,  the difference was not statistically significant (P > 0.05). 3 d after treatment, the PaCO2 in SIMV group [(36.21±4.38) mmHg] was significantly lower than that in CPAP group [(42.54±5.19) mmHg], the difference was statistically significant (P < 0.05), while the PaO2, SpO2 and OI [(63.29±5.19) mmHg, (0.95±0.08), (239.54±36.41)] were significantly higher than those in CPAP group [(57.19±5.09) mmHg, (0.91±0.09), (222.96 ±32.48)], the differences were statistically significant (P < 0.05). The duration of ventilation, hospital stay, the proportion of intubation and ventilator setting, the total incidence of complications in SIMV group [(34.12±10.78) h, (15.13±4.29), 8.33%, 20.00%] were compared with CPAP group [(48.75±12.49) h, (18.64±5.31) d, 25.00%, 44.64%], the differences were statistically significant (P < 0.05). Conclusion The curative effect of SIMV is obvious in neonates with respiratory failure. It can improve blood gas indexes, shorten the duration of ventilation and reduce intubation and complications.
2018 Vol. 15 (13): 72-75 [Abstract] ( 218 ) HTML (1 KB)  PDF (648 KB)  ( 65 )
76 Effect of laser coagulation for the stress response and prognosis of patients with varicose of great saphenous vein
WANG Tao1 YANG Hongyu2
Objective To explore the effects of laser coagulation for the stress response and prognosis of patients with varicose of great saphenous vein. Methods Ninety-six patients with varicosis of great saphenous vein admitted to Jiangning Hospital Affiliated to Nanjing Medical University from January 2014 to June 2017 were selected as research objects. They were divided into observation group and control group according to random number table, with 48 cases in each group. Patients in the control group received the high ligation of great saphenous vein and varicose vein exfoliation, while the observation group received the laser coagulation. The surgery-related indicators (operation time, number of incision, intraoperative blood loss, postoperative hospital stay), stress response before and after operation, complications and the recurrence rate after follow-up for 12 months were compared between the two groups. Results The operation time, number of incision, intraoperative blood loss, postoperative hospital stay in the observation group were significantly lower than those in the control group (P ＜ 0.01). After operation for three days, the serum levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and NO in the observation group were significantly lower than those in the control group (P ＜ 0.01), and the incidence of incision infection, local hematoma ecchymosis, saphenous nerve damage were lower than those in the control group (P ＜ 0.05). After 12 months of follow-up, the recurrence rate of the two groups was not statistically significant (P ＞ 0.05). Conclusion The laser coagulation can help relieve the surgical stress response, promote the recovery and reduce the postoperative complications in patients with varicosis of great saphenous vein.
2018 Vol. 15 (13): 76-79 [Abstract] ( 226 ) HTML (1 KB)  PDF (693 KB)  ( 53 )
80 Effect of low central venous pressure combined with acute hypervolemic hemodilution on pulmonary shunt and arterial oxygen pressure in patients with thoracic surgery
HU Zhengrong1 WEI Binhua1 LI Changke1 TAN Jiangbo2 WU Qinru3
Objective To investigate the effect of low central venous pressure (LCVP) combined with acute hypervolemic hemodilution (AHHD) on intrapulmonary shunt and arterial oxygen partial pressure during one lung ventilation in thoracic surgery. Methods From January 2015 to December 2017, ASA grade Ⅰ-Ⅱ undergoing elective radical resection of lung cancer and 50 thoracic surgery patients in the Affiliated Yuebei People's Hospital, Shantou University Medical College, were randomly divided into two groups: group AHHD with LCVP (group LA) and control group (group C), each group had 25 cases. Group LA was given the limited crystalloid the amount of nitroglycerin and continuousmicro pump injection method after anesthesia in advance of LCVP technology, control of CVP in 2-5 cmH2O, then combined with the application of AHHD technology, rapid infusion of 6% hydroxyethyl starch (130/0.4) 15 mL/kg on blood thinners, keep Hct at 25%-30%. Group C was maintained at the normal level of 6-12 cmH2O CVP. On the each time as 10 min after double lung ventilation in the supine position (T0), 10 min after one lung ventilation in supine position (T1), 10 min after single lung ventilation in lateral position (T2), 10 min after pulmonary lobectomy (T3), the blood glucose and cortisol level were measured by collecting, and the blood of the elbow. The radial artery blood and right atrial mixed venous blood was collected for blood gas analysis, and record blood glucose concentration, serum cortisol level, SpO2, HR, MAP, CVP, BIS, PetCO2, arterial oxygen content (CaO2), mixed venous oxygen content (CvO2), arterial and mixed venous blood oxygen partial pressure (PO2), arterial and mixed venous blood oxygen saturation of hemoglobin (SO2), arterial and mixed venous blood hemoglobin (Hb) etc.. The rate of intrapulmonary shunt (Qs/Qt), Qs/Qt = (CcO2-CaO2)/(CcO2-CvO2)×100% were calculated according to the standard three compartment model of pulmonary blood flow distribution. Results The blood glucose concentration, serum cortisol level, SpO2, HR, MAP, BIS, PetCO2 of patients in two groups at each time point was basically stable, but after one lung ventilation, LA group and group C significantly increased intrapulmonary shunt (P < 0.05), which increased by 13.7% and 14.9% respectively in supine position, lateral position increased 11.4% and 14.3% respectively. arterial oxygen partial pressure of two groups were significantly decreased (P < 0.05), and compared with the supine lateral position decreased more obviously, but there was no significant difference between the two groups (P > 0.05). After lobectomy, the intrapulmonary shunt in group LA and group C was significantly reduced (P < 0.05), 7.2% and 8.6% respectively, and the arterial oxygen partial pressure increased significantly in two groups (P < 0.05), but there was no significant difference between the two groups (P > 0.05). The proportion of blood transfusion in group LA was significantly lower than that in group C (χ2 = 4.902, P < 0.05). Conclusion Low central venous pressure combined with acute hypervolemic hemodilution has no significant inhibition on intrapulmonary shunt and arterial oxygen partial pressure during one lung ventilation during thoracic surgery, and can significantly reduce the proportion of intraoperative blood transfusion.
2018 Vol. 15 (13): 80-84 [Abstract] ( 207 ) HTML (1 KB)  PDF (492 KB)  ( 134 )
85 Effect of different concentrations of the incidence and onset time of rate of Fentanyl-induced cough
WANG Maohua ZHANG Jing ZHAO Mengya CHEN Maogui ZHANG Zhuan SUN Xiaohong SUN Jianhong
Objective To investigate the effect of different concentrations of the incidence and onset time of rate of Fentanyl-induced cough. Methods From January to November 2017, 300 patients with general anesthesia for elective surgery in Affiliated Hospital of Yangzhou University were selected as research subjects. They were randomly divided into six groups using a random number table, non dilution control group A (group A1). One times dilute group A (group A2)︰ Fentanyl and normal saline 1︰1 were diluted and was uniform; 2 times dilute group A (group A3)︰ Fentanyl and normal saline 1︰2 were diluted and was uniform, and those three groups were injected at 5 s, non dilution control group B (group B1). One times dilute group B (group B2)︰ Fentanyl and normal saline 1︰1 were diluted and was uniform; 2 times dilute group B (group B3)︰ Fentanyl and normal saline 1︰2 were diluted and was uniform, and those three groups were injected at 10 s, with 50 cases in each group. The time of cough and the incidence rate of cough among the six groups were recorded and compared. Results When the concentration of the drug was the same and the speed of drug delivery was different, the cough onset time in group B1 were significantly longer than group A1 (P < 0.05). The incidence of cough in group B1 were significantly lower than group A1 (P < 0.05), the cough onset time in group B2 were significantly longer than group A2 (P < 0.05). The incidence of cough in group B2 were significantly lower than group A2 (P < 0.05), the cough onset time in group B3 were significantly longer than group A3 (P < 0.05). When the speed of drug delivery was the same and the concentration of the drug was different, compared with group group A1, there was no statistically significant differences of cough onset time in group A2 and group A3 (P > 0.05), the incidence of cough in group A2 were lower than group A1 (P ＜ 0.05), and the incidence of cough in group A3 were significantly lower than group A1 and group A2 (P < 0.05). Compared with group group B1, there was no longer of the cough onset time in group B2 (P > 0.05), and the incidence of cough in group B2 were lower than group B1, the differen (P < 0.05ce was statistically signifincant). Compared with group B1 and group B2, the cough onset time in group B3 were significantly longer than group B1 and group B2, and the incidence of cough in group B3 were significantly lower than group B1 and group B2, the differences were statistically significant (P < 0.05). Conclusion Reducing the speed of drug delivery and concentration of Fentanyl, which can prolonging the time of the cough onset time, reduce the incidence of cough, when the Fentanyl and normal saline 1︰2 were diluted and was uniform, which can significant inhibition of cough, thus it is significance to improve the safety of the patients′ anesthesia.
2018 Vol. 15 (13): 85-88 [Abstract] ( 202 ) HTML (1 KB)  PDF (476 KB)  ( 174 )
89 Oxycontin combined with Morphine Tablets in the treatment of cancer pain and its effect on the quality of life of patients
SONG Jianming1 LI Bin2▲
Objective To analysis of Oxycontin combined with Morphine Tablets in treatment of cancer pain and its effect on the quality of life of patients. Methods From August 2016 to September 2017 in Qianjiang Central Hospital of Chongqing, 100 patients with cancer pain were randomly divided into observation group and control group, with 50 patients in each group. The control group was treated with Oxycontin, the observation group was treated with Oxycontin combined with Morphine Tablets. Before and after treatment, the visual analogue score （VAS） was used in the assessment of the clinical efficacy in the treatment of patients, the QLQ-C30 scale was used to evaluate the life quality of the patients, the ECOG-PS physical status scale was used for patients′ physical condition evaluation, statistical analysis of the clinical curative effect and adverse reactions of patients was carried out. Results There was no significant difference between the two groups of visual analogue score （VAS）, QLQ-C30 and ECOC-PS before treatment （P > 0.05）. The scores of VAS, QLQ-C30 and ECOC-PS in the two groups were significantly improved after treatment （P < 0.05）, and the VAS, QLQ-C30 and ECOC-PS scores of the patients after treatment in the observation group were significantly better than those of the control group （P < 0.05）. The analgesic efficacy of the observation group was significantly higher than that of the control group （P < 0.05）, and the incidence of nausea, vomiting, dizziness, constipation and dysuria was significantly lower in the observation group than that in the control group （P < 0.05）. Conclusion The Oxycontin combined with Morphine Tablets for the treatment of cancer pain patients can alleviate the pain symptoms of patients, and effectively improve the quality of life of patients, has high clinical application value.
2018 Vol. 15 (13): 89-92 [Abstract] ( 694 ) HTML (1 KB)  PDF (499 KB)  ( 74 )
93 Clinical effect of Wenjing Plaster combined with Union Jack type cervical exercise in the treatment of cervical spondylotic radiculopathy
YANG Yanyang1,2 CHEN Xiaoyong3
Objective To investigate the clinical effect of Wenjing Plaster with Union Jack type cervical exercise in the treatment of cervical spondylotic radiculopathy. Methods Sixty cases of patients with cervical spondylotic radieulopath admitted to Outpatient Department of Orthopaedics, Sanya Hospital of Traditional Chinese Medicine from January 2016 to August 2017 were selected, and they were divided into treatment group and control group by random number table, with 30 cases in each group. The treatment group was given external application of Wenjing Plaster on the neck combined with Union Jack type cervical exercise, while the control group received traditional Chinese medicine hydropathic compress on the neck with the addition of visible light and Union Jack type cervical exercise. After treatment for 2 weeks, the neck pain visual analogue scale (VAS), neck disability index (NDI), total effective rate, and recurrence rate after 3 months in the two groups were observed. Results After treatment for 2 weeks, the neck pain VAS scores and NDI in the two groups were all significantly lower than those before treatment (P ＜ 0.01), and the neck pain VAS scores and NDI of treatment group were lower than those of control group, the differences were highly statistically significant (P ＜ 0.01). After treatment for 2 weeks, the total effective rate of the treatment group (86.67%) and control group (73.33%) had no statistically significant difference (P ＞ 0.05). Three months later, the recurrence rate of the treatment group (7.69%) was lower than that of the control group (36.36%), the difference was statistically significant (P ＜ 0.05). Conclusion Wenjing Plaster combined with Union Jack type cervical exercise in the treatment of cervical spondylotic radiculopathy has obvious efficacy, which can improve cervical function in a certain extent, and the total effective rate is relatively satisfactory, the recurrence rate is relatively low.
2018 Vol. 15 (13): 93-96 [Abstract] ( 223 ) HTML (1 KB)  PDF (562 KB)  ( 86 )
97 Construction and practice of education system for information management and information system major based on advantages and characteristics of Chinese medicine subject
MI Li1 WANG Zhenguo2 HE Yong1 LI Xuebo1 WANG Ruixiang1
At present, 42 medical colleges and universities in China (9 Chinese medicine colleges) have set up the information management and information system major. In the Chinese medicine colleges, unclear students training target causes the mismatching between students′ training and the development of traditional Chinese medicine (TCM). Because of the multidimensional main courses (economics, management, computer science and background courses of TCM), the curriculum system is disintegrated. At the same time, the theory of information management and information system technology has become an important support and protection in the field of TCM that calls for students with the ability of practice, application and innovation. Since the establishment of information management and information system specialty in 2006, the education idea of highlighting characteristic and innovation were put forward, and the college carried out some research and innovation in the training project, curriculum system, teaching mode, theory, practice and support platform, having improved the “three stage penetration” courses teaching mode of knowledge learning. Course practice and job practice, formed the “multi-leading” practice teaching mode such as Chinese literature, Chinese medicine information, information technology, information management. The information management and information system specialty has constructed abundent ways to strengthen the platform for innovation, forming the multi-level student training system.
2018 Vol. 15 (13): 97-100,104 [Abstract] ( 246 ) HTML (1 KB)  PDF (664 KB)  ( 58 )
101 Relevant theories discussion on the incidence of depression and yang qi in the five internal organs
WANG Liyuan1 ZHU Weining2 SHI Ling3
Yang qi is the foundation of human spirit life activity, which plays a crucial role in our life. The adequate yang qi ensures the orderly life metabolism. On the contrary, insufficient yang qi will weaken or decrease the function of viscera, and make the ascending and descending functional activities of qi abnormal. It will thus affect the five minds and cause a series of complex emotional diseases. As a common thymopathy, depressive disorder is classified as the category of depression in traditional Chinese medicine. It is believed that the pathogenesis of depression is closely related to yang qi, and is the product of insufficiency of yang qi and low dynamic state. Meanwhile, this article discusses that the insufficiency of the five internal organs is closely related to the occurrence of depression, and analyzes theoretically in detail. Combining with the etiology and pathogenesis, this article puts forward a basic depression treatment principle based on warming yang and regulating qi and related viscera function. Common symptoms are summarized in clinical diagnosis and treatment, so as to provide an effective basis for the clinical prevention and treatment of the disease.
2018 Vol. 15 (13): 101-104 [Abstract] ( 171 ) HTML (1 KB)  PDF (510 KB)  ( 71 )
105 Application in the evaluation of neoadjuvant chemotherapy for breast cancer with contrast-enhanced ultrasonography combined with color doppler ultrasound
HAN Yanfeng1 ZOU Shuwei2 WANG Baohua3▲
Objective To discuss the value in the neoadjuvant chemotherapy for breast cancer with contrast-enhanced ultrasonography and color Doppler ultrasound. Methods From March 2016 to March 2017, 65 cases of breast cancer patients in Chinese and Western Medicine Hospital of Taizhou were selected as the observe object, all patients underwent neoadjuvant chemotherapy (NAC), ultrasound imaging and color Doppler ultrasound (CDFI) examination were used before and after NAC. The value of contrast-enhanced ultrasonography, CDFI ultrasonography and combined application in assessing the efficacy of NAC in breast cancer were observed. Results According to the Miller and Payne pathology classification criteria, all the patients could be divided into chemotherapy (54 cases) and ineffective (11 cases) after NAC treatment. The sensitivity of CDFI was 79.25%, the specificity was 33.33%, the sensitivity of the dual-mode and CDFI ultrasound was 96.23%, the specificity was 75.00%. The accuracy of the dual-mode and ultrasound examination was significantly higher than CDFI, the results of the coherence analysis showed that the Kappa value was 0.664, the patients underwent CEUS before and after NAC. There was no significant difference in time (TTP) and enhancement time (MTT), the differences was not statistically significant (t=1.052, 1.179, all P > 0.05), the peak intensity (PI) and area under the curve were significantly lower after chemotherapy than before NAC, the differences were statistically significant (t = 4.513, 125.631, all P < 0.05), pathological assessment of NAC effective relief of peak intensity changes was significantly higher than ineffective patients, the difference was statistically significant (t = 5.916, P < 0.05). The ROC curve showed that is the most accurate when resolving NAC validity by the peak intensity change rate corresponding to the sum of sensitivity and specificity was used as the cutoff value. Conclusion Dual-mode contrast-enhanced ultrasonography combined with CDFI examination can accurately assess the effect of breast cancer NAC treatment, relative to the CDFI evaluation, the results are more reliable, accurate, it can be widely used in the clinical.
2018 Vol. 15 (13): 105-108 [Abstract] ( 739 ) HTML (1 KB)  PDF (506 KB)  ( 108 )
109 Application of multi-slice spiral computed tomography angiography for diagnosis of left main coronary disease
GUO Zhen1 ZHAO Bin1 YUAN Yonggang2 YU Shujing3 XU Zesheng2▲
Objective To compare the values of multislice spiral computed tomography angiography (MSCTA) and CAG in diagnosis of left main coronary artery (LMCA) lesion, and the value of MSCTA in the qualitative analysis of LMCA plaques. Methods A total of 359 patients examined with MSCTA and CAG in Cangzhou Central Hospital from October 2012 to January 2015 were selected as research objects. The LMCA lesion was divided into: the proximal part, the middle part, and the distal part. Then the sensibility, specificity and consistency in diagnosis of LMCA lesion using MSCT were compared, with CAG as the gold standard. Results Through the comparison, the results were similar in the diagnosis of the proximal, the middle part between MSCTA and CAG, without statistically significant difference (P > 0.05). The sensitivity, specificity, false negative rate for diagnosis in the distal part were 91.2%, 99.4%, and 6.1%, Kappa index was 0.917 (P > 0.05). Conclusion MSCTA has high accuracy in diagnosis of LMCA lesion and it can be used for qualitative analysis of LMCA plaques．The results are similar in the diagnosis of the proximal and middle part. The accuracy of MSCT in the diagnosis of proximal LMCA lesions is higher than that of the distal lesions．
2018 Vol. 15 (13): 109-112 [Abstract] ( 276 ) HTML (1 KB)  PDF (411 KB)  ( 167 )
113 Efficacy of Atorvastatin combined with Dimethylbiguanide in treating elderly type 2 diabetes with dyslipidemia
WANG Yonghai1 JIANG Meiju1▲ WANG lin2 ZHOU Hongli1
Objective To investigate effect of Atorvastatin combined with Dimethylbiguanide in treating elderly type 2 diabetes with dyslipidemia. Methods The clinical data of 280 cases of elderly patients diagnosed as type 2 diabetes with dyslipidemia in Chengyang People's Hospital from January 2014 to January 2017 was analyzed, they were divided into two control group (Atorvastatin) and observation group (Atorvastatin combined with Dimethylbiguanide), with 140 cases in each group. The levels of fasting blood glucose (FBG), postprandial blood glucose (PBG), glycosylated hemoglobin (HbA1c), fasting insulin (Fins), insulin resistance (HOMA-IR), triacylglycerol (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterin (LDL-C) of two groups were detected before and after treatment. The clinical effects of the two groups were observed. Results The levels of FBG, PBG, HbA1c, Fins, HOMA-IR, TG, TC, HDL-C, LDL-C between two groups had no significant differences before treatment (P ＞ 0.05). After treatment, the levels of FBG, PBG, HbA1c, Fins, HOMA-IR, TG, TC, LDL-C in the two groups were all significantly lower than those before treatment, while the levels of HDL-C were higher than those before treatment (P ＜ 0.05). After treatment, the levels of FBG, PBG, HbA1c, Fins, HOMA-IR, TG, TC, LDL-C of observation group were lower than those of control group, while the level of HDL-C was higher than that of control group (P ＜ 0.05). The total effective rate of observation group was higher than that of control group, the difference was statistically significant (P ＜ 0.05). Conclusion Atorvastatin combined with Dimethylbiguanide in treating elderly type 2 diabetes with dyslipidemia can significantly improve the levels of blood glucose and blood lipid, the clinical efficacy is remarkable and it is worthy of promotion and application.
2018 Vol. 15 (13): 113-116,155 [Abstract] ( 235 ) HTML (1 KB)  PDF (706 KB)  ( 127 )
117 Enlightenment of American graduate medical education system on Chinese nephrology specialty education
BAI Ming LI Lu LI Yangping ZHAO Lijuan WANG Huang SUN Shiren
Specialty education is one of the most important stages of thetraining of high-quality physicians. The prevalence of chronic kidney disease in China is 10.8%. There is short of nephrology specialties for the supply of high quality medical services. The American specialty education system is one of the advanced system for graduate medical education. This article analyzes the strategies of American nephrology specialty education, including the organization model, training time, training targets, training arrangement, and training evaluation strategies. Furthermore, it presentes several suggestions for the nephrology specialty education of our country and believes that the American strategies of the training time and training evaluation have important reference value for the construction of our training strategies. Of course, the nephrology specialty training model of our country should adapt the current situation of our hospitals and have Chinese characteristics.
2018 Vol. 15 (13): 117-120 [Abstract] ( 291 ) HTML (1 KB)  PDF (626 KB)  ( 66 )
121 The explore in training mode of clinical pharmacists in Department of Respiration
LIANG Xiuqun LI Jianzhe LU Cuilin CHEN Qingjie LI Zhenfu LIU Sixiang
To provide reference for the training of clinical pharmacist in Respiratory Department, the author reviewed the work patterns and method in clinical practice, and summarized the training mode of respiratory clinic pharmacist based on clinical practice. The author think the initial training of clinical pharmacists in Respiratory Department should take a textbook-based and problem-oriented case teaching method, and combine the relevant guidelines and expert consensus to systematically study medical knowledge and pharmacy knowledge, participate in drug treatment and help physicians choose more rational drug treatment programs, and gradually establish clinical thinking and clinical pharmacy thinking, improve the ability of the application of drugs. At the same time, through the search of literature, standard medicine calendar, case analysis, literature reading reports and other pharmaceutical instruments to improve oral expression and communication skills. By continuously improving the training mode and assessment standards, we can better cultivate clinical pharmacists suitable for clinical needs.
2018 Vol. 15 (13): 121-125 [Abstract] ( 219 ) HTML (1 KB)  PDF (690 KB)  ( 95 )
126 Exploration and research on curriculum construction of Chinese medicine culture
FU Wendi
Traditional Chinese medicine discipline is the comprehensive summary of traditional Chinese medicine culture, which is systematic and theoretical. In current situation, traditional Chinese medicine is highly valued by our country, which speeds up the college education development of Chinese medicine that regards Chinese medicine culture as the breakthrough and brings about great opportunities for Chinese medicine culture. Although Chinese medicine culture discipline develops in a fast way, it is in the spontaneous and free construction stage, which has no consensus about some basic problems in Chinese medicine culture. The construction of Chinese medicine culture discipline should be considered from the aspects of theory construction, practice exploration and future development. Its basic theory, course exploitation, technology integration and method creation should be paid more attention. Guiding ideology, basic content, teaching method and education for teachers should be the breakthrough, which makes the course construction of Chinese medicine culture be the pioneer of Chinese medicine education reform. It contributes to the cultural heritage of Chinese medicine.
2018 Vol. 15 (13): 126-130 [Abstract] ( 252 ) HTML (1 KB)  PDF (638 KB)  ( 153 )
131 Enlightenment of narrative medicine on medical ethics education of young medical student in China
DUAN Jinning1 HAN Shuangxue2 CAO Xisheng3 WEI Hong4▲
The concept of narrative medicine has been put forward for nearly 15 years, and has been recognized to a large extent in western developed countries such as the US, Europe and Asia. Firstly, this paper analyzes the current situation and problems of medical ethics education among medical students of our country, and illustrates idea and application area of narrative medicine. It introduces in detail the ethics of narrative medicine ethics education strategy based on young medical students, suggests that we should carry out the evaluation of medical ethics and medical ethics in clinical teaching and clinical practice, and establish and implement the medical ethics and medical ethics reward and punishment plan. This paper hopes to provide reference for the application of the domestic field of medical ethics education narrative.
2018 Vol. 15 (13): 131-134 [Abstract] ( 735 ) HTML (1 KB)  PDF (631 KB)  ( 247 )
135 Research on the ecological environment of computer teaching in traditional Chinese medicine colleges and universities based on virtualization
LI Jingzhong WANG Ping LI Jianzhi LI Xiaoyan▲
The traditional computer teaching ecological environment information system is scattered, teachers and students are lack of interaction; the laboratory management is tedious and the training teaching is difficult to carry out. Based on the analysis of virtualization technology, it is considered that the combination of server virtualization technology and desktop virtualization technology are the best choice to improve the computer teaching environment, which can improve the training effect, reduce the cost of hardware and software operation and infrastructure integration. It has constructive meaning for the reform of computer teaching and the cultivation of students' comprehensive ability.
2018 Vol. 15 (13): 135-138 [Abstract] ( 211 ) HTML (1 KB)  PDF (1103 KB)  ( 59 )
139 The practice and exploration of scientific research training program based on the cultivation and promotion of college students′ innovation ability
XU Qi1 MA Xiaomei2 GU Danjin1 WAN Ming1 ZHOU Yong1 CHENG Lufeng1
The innovation education of college students has become the inevitable requirement and the direction of the future development of higher education. Student research training program is an important segment in the training system. This paper makes a useful exploration on the development of SRTP based on the research project of the tutor. Practice proved that relying on scientific research projects, enable the students to initially establish scientific research thinking, clear research objectives, cultivate and enhance scientific research ability, and produce better innovative results. At the same time, it can produce good innovation results and play a good foundation role for students' undergraduate and follow-up study. It is benefit the talent cultivation.
2018 Vol. 15 (13): 139-142 [Abstract] ( 170 ) HTML (1 KB)  PDF (633 KB)  ( 117 )
143 Innovation of hospital safety management committee in complaint handling
ZHANG Taojing HU Kaiwen ZHAO Chang▲ XU Weina ZHANG Huishu
With the new stage of medical reform, constructing a harmonious doctor-patient relationship has become the focus of the work of every hospital. Dongfang Hospital, Beijing University of Chinese Medicine, established a safety management committee system in 2014. As a result, patients′ problems are solved effectively, the complaint handled process standardized, the prevarication is reduced and the dispute resolution channels are unimpeded. What is more the amount of medical compensation has decreased by 89% and complaints have decreased by 11%. Meanwhile, the patients′ satisfaction is greatly improved. A harmonious doctor-patient relationship is constructed. This paper introduces the establishment, the organization structure and the management mode of safety management committee system in Dongfang Hospital, in order to share the practical experience with other hospitals for reference.
2018 Vol. 15 (13): 143-146 [Abstract] ( 222 ) HTML (1 KB)  PDF (531 KB)  ( 135 )
147 Structural evaluation of 2014-2016 hospitalization expenses in a grade Ⅲ level A general hospital based on structural variability degree
FENG Jun1 LI Yingli1,2▲ XU Tingting1 GAO Hongda1 QIN Xianjing1 FENG Qiming1 WANG Jianzheng3
Objective To analysis the trend and characteristics of hospitalization expenses structure change in a grade Ⅲ level A general hospital, and provide evidence for the unreasonable increase of hospitalization expenses. Methors The hospitalization expenses of patients in a grade Ⅲ level A general hospital from 2014 to 2016 were collected by analyzing the structure changes value, degree of structure variation and structure contribution rate. Results The total hospitalization expenses of the top three hospitals increased year by year from 2014 to 2016, but the growth rate decreased. The proportion of medical expenses and medical materials expenses accounted for the total hospitalization expenses of top 2. The value of negative changes in structure of 2014-2016 medicine fees, examination fees, examination fees, bed charges, treatment fees and nursing; value positive changes in the structure of health laboratory fees, material fee, operation fee and other expenses. The cost of medicine and medical materials contribute greatly to the change of hospitalization expenses. The cumulative contribution rate of these 2 items was 47.06%. Conclusion Expenses control policies for medicine has achieved initial success, but the proportion of medicine expenses is still high which still need to strengthen the medicine expenses control. The structure of hospitalization expenses is unreasonable. The proportion of medical materials and laboratory fees are high. However, the cost of operation and nursing care which reflects the technical labor value of medical staff is relatively low. Expenses structure needs to be adjusted and optimized.
2018 Vol. 15 (13): 147-150 [Abstract] ( 196 ) HTML (1 KB)  PDF (477 KB)  ( 69 )
151 Ppractice and solution path of hospital risk management
ZHOU Wei HU Pingling LIANG Yaoze LIAO Shengwu
Hospital risk management is a managerial process which studies potential risk links during medical service, reduces uncertain factors and minimizes malpractice, so as to ensure the quality of medical care. Through summarizing experience and shortages of risk management at home and abroad, as well as integrating risk management practice in outpatients′ critical value notification, preoperative condition assessment, surgical patient transfer, surgical site identification, surgical safety verification, and other high risk procedures, the solutions and suggestions of risk management are conducted. More importantly, medical risk management needs to establish a comprehensive risk management system from the decision-making level, manager and executive level. Based on the evaluations of key quality assessment and surveillance, by utilizing the skills of risk identification, risk assessment, risk control and risk tracking, as well as applying medical quality management methods and tools, the optimal goal of this research is to ensure patients′ safety, build a comprehensive, long-term, continuously improving hospital quality management system.  
2018 Vol. 15 (13): 151-155 [Abstract] ( 179 ) HTML (1 KB)  PDF (781 KB)  ( 95 )
156 Effect of objective nursing mode in chronic obstructive pulmonary disease patients with diaphragm pacemaker adjuvant therapy
DAI Yan LIU Yunqiu▲ GOU Haichao YANG Min MA Hongfang SONG Lu
Objective To explore the application effect of target nursing mode in adjuvant therapy of EDP in AECOPD patients. Methods From March 2015 to April 2017, in Kailuan General Hospital, 200 AECOPD patients undergoing EDP therapy were selected, according to random number table, they were divided into target group (n = 100, given routine nursing and nursing target) and convention group (n = 100, given routine nursing). The negative emotional scores, chronic obstructive pulmonary symptom score (CAT), exercise endurance, and lung function indexes and the degree of satisfaction were compared. Results After implementation, the scores of anxiety and depression, CAT in two groups were lower than those before implementation (P < 0.05), and these in the target group were lower than those in the routine group (P < 0.05). After implementation, the 6 min walking distance (6MWT), the respiratory volume (FEV1) and forced expiratory volume (FEV1/FVC) in two groups were higher than those before implementation (P < 0.05), these in the target group were higher than those in the convention group (P < 0.05). The satisfaction and the good rate in two groups were compared, the difference was not statistically significant (P < 0.05). Conclusion In the process of EDP assisted treatment, the AECOPD targeted nursing mode can significantly relieve negative emotions, improve clinical symptoms, exercise endurance and lung function, and enhance nursing satisfaction.
2018 Vol. 15 (13): 156-159 [Abstract] ( 200 ) HTML (1 KB)  PDF (600 KB)  ( 67 )
160 Effect of nursing intervention on reducing incidence of peritoneal dialysis associated peritonitis
MA Miguo1 HE Xin2 CHEN Taiai1
Objective To integrative the effect of nursing intervention on reducing incidence of peritoneal dialysis associated peritonitis. Methods From January 2014 to December 2015, in Department of Nephrology, People's Hospital of Qionghai City, 87 patients with peritoneal dialysis were selected, according to random number table method, they were divided into the observation group (44 cases) and control group (43 cases). The observation group was given the nursing intervention established by the hospital. The control group was given routine nursing care. The incidence of peritonitis associated peritoneal dialysis in the two groups were compared. Results Among 87 patients, 28 cases occurred peritonitis, with a rate of 32.18%. The incidence of the observation group (8 cases) was significantly lower than the control group (20 cases) (P < 0.05). The main cause of peritonitis associatation peritoneal dialysis was non-strict aeptic operation (50.0%). In 28 cases, 19 patients (67.86%) had positive bacterial culture, 9 cases (47.37%) were gram positive bacteria, 7 cases (36.84%) were gram negative bacteria, and 3 cases (15.79%) were fungi. Gram positive bacteria was susceptible to Vancomycin, and the resistance rate was 0.00%. Gram negative bacteria was sensitive to Imipenem, and the resistance rate was 0.00%. Conclusion Peritoneal dialysis related peritonitis has a lot of causes. The nursing intervention can effectively reduce the risk of peritonitis, improve the life quality of patients, and protect the peritoneal function of patients. It plays a positive role in the treatment of patients with peritoneal dialysis.
2018 Vol. 15 (13): 160-163 [Abstract] ( 189 ) HTML (1 KB)  PDF (645 KB)  ( 146 )
164 Construction of practical training system of traditional Chinese medicine nursing talents in Nanjing based on Delphi method
GUO Xiujun1 WU Qiong1 MENG Yahui1 Zhang Huiling2 XU Guihua3 HONG Yanyan1 DU Yuan1
Objective To construct a training system of practice TCM nursing talents that meets the actual needs of clinical practice, and to provide a scientific theoretical framework for the development of TCM nursing personnel training. Methods Through literature study, expert interview, group discussion, form the practice of traditional Chinese medicine nursing personnel training system questionnaire, the Delphi method was adopted for 15 nursing experts from 8 provinces and cities in 2 rounds of experts enquiry. Results The recovery rate of the questionnaire was 100% and 80% respectively, and the expert authority coefficient was 0.86, 0.84, and the Kendall harmonious coefficient was 0.24 and 0.32 respectively, and the difference was statistically significant (P < 0.01). Six first-level entries, 17 secondary entries and 113 tertiary entries were selected. The maximum weight of practical courses was training and then were theory course,teacher′s condition, inclusion object and training target respectively. Conclusion The results of this study are highly reliable, scientific and operable, and provide an operable theoretical framework for the further development of practical TCM nursing personnel training.
2018 Vol. 15 (13): 164-167 [Abstract] ( 220 ) HTML (1 KB)  PDF (650 KB)  ( 108 )
168 Correlation analysis between emotional regulatory belief and the self-concept level of nursing specialty in operating room nursing
LI Jingxian1 WANG Li2▲ YONG Jie3 YI Min4 DONG Xiaomei5
Objective To investigate the level of emotional regulation and nurses' self-concept in operating room nurses, and to explore the correlation between them. Methods A total of 102 nurses in operating room from 4 hospitals in Dandong, Liaoning Province were selected by convenient sampling method in March 2016, and the level of emotional regulation and nurses' self-concept were tested by the emotion control beliefs scale (ERBS) and the nursing professional self concept scale (PSCNI). All subjects were divided into professional attitude positive group (54 cases, positive group) and the professional attitude negative group (48 cases, negative group) according to the evaluation results of PSCNI. Relevant data was collected and collated, and their association was analyzed. Results ①In this study, the level score of emotion control belief of nurses in operating room was (82.57±4.73) scores, and the self-concept level of nursing specialty was (77.86±4.51) scores. ②The total score and dimension of PSCNI in positive group were higher than that in negative group (P < 0.05). The scores of flexibility, skills, communication and satisfaction in positive group were higher than those in negative group (P < 0.05). ③The ERBS scores was higher, and motional constraint and emotional stress were lower in positive group than those in negative group (P < 0.05). ④Pearson correlation analysis showed that PSCNI total scores was positively correlated with ERBS scores, and the self-concept level of nursing was positively correlated with emotion regulation value, and was negatively correlated with emotion constraint and emotion stress (P < 0.05). Conclusion The level of emotional regulatory beliefis positively correlated with self-concept level of nursing specialty in operating room nurses, and the higher level of emotion regulationbelief can promote the improvement of self-concept level of nursing profession, and promotes healthy and positive occupational attitudein operating room nurses together.
2018 Vol. 15 (13): 168-172 [Abstract] ( 206 ) HTML (1 KB)  PDF (656 KB)  ( 147 )
173 Study on cognitive status of nursing students on hospital injury and its correlation with occupational values and employment trends
LI Li1 PEI Hualian2 WU Xing3▲ WANG Meixin3
Objective To explore the influence of hospital injury doctors on the professional values and employment trend of interns from different nationalities. Methods In June 2017, three questionnaires were distributed to 300 nursing students of master, undergraduate and specialty in First Affiliated Hospital of Xinjiang Medical University including the cognition questionnaire of nursing students to hospital injury doctors, the occupational values scale of nursing students and the questionnaire of employment tendency, and the correlation and regression analysis were carried out on the results of the questionnaire. Results There was correlation between the employment tendency of interns and the language attack, physical attack and the time of occurrence of injury medical events (P < 0.05), there was no correlation between the employment tendency of interns and nation (P > 0.05). There were correlations between the professional values of interns and their education level, language attack, physical attack, sexual harassment and time of occurrence (P < 0.05). There was correlation between the employment trend of nursing students and their professional values (P < 0.05). Conclusion Hospital injury events have a close impact on the professional values and employment trends of interns, there is no ethnic differences in Xinjiang.
2018 Vol. 15 (13): 173-176 [Abstract] ( 228 ) HTML (1 KB)  PDF (646 KB)  ( 135 )
177 Effect of phacoemulsification for cataract with high myopia combined with cooperation procedures for intraocular lens implantation
QIU Aizhu HOU Lihuan▲ HE Xiaojuan CHEN Daoting HUANG Xiaohong
Objective To discuss the effect of Phacoemulsification for cataract with high myopia combined with cooperation procedures for intraocular lens implantation. Methods Forty cases patients with high myopia and cataract surgeries (40 eyes) in the department of Ophthalmology, the First Affiliated Hospital of Ji′nan University whose operation were performed by the same surgeon doctor from February 2014 to September 2015 were selected and divided into experimental group and control group by random number table, with 20 cases patients (20 eyes) in each group. Patients in the control group were treated with  routine operation and procedure nursing care; while the patients in experimental group were treated with routine operation and improved operation. The improvement of intraoperative distention and pain, the time of operation, the incidence of corneal edema after operation, doctor and patient satisfaction of the patients in the two groups were compared. Results Compared with the control group, the improvement of intraoperative distention and pain, the time of operation, the incidence of corneal edema after operation, doctor and patient satisfaction of the patients in experimental group were significantly better than control group, the difference had statistical significant (P < 0.05). Conclusion To improve the operation coordination rules can significantly shorten the operation time, reduce postoperative complications, improve the satisfaction of doctors and patients.
2018 Vol. 15 (13): 177-180 [Abstract] ( 252 ) HTML (1 KB)  PDF (645 KB)  ( 97 )
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