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| The effects of non-myeloablative conditioning and cord blood hematopoietic stem cells transplantation on spermatogenic function in mice |
| ZHANG Meng1* YAO Guanping2* HE Fang1 LI Yuxiang3 FAN Zhenhai1 YU Limei1 |
1.Key Laboratory of Cell Engineering in Guizhou Province, Affiliated Hospital of Zunyi Medical University, Guizhou Province, Zunyi 563000, China;
2.Human Reproductive Medicine Center, Affiliated Hospital of Zunyi Medical University, Guizhou Province, Zunyi 563000, China;
3.Department of Phylaxiology and Pediatric Dentistry, Hospital of Stomatology, Zunyi Medical University, Guizhou Province, Zunyi 563000, China |
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Abstract Objective To explore the effects of chemotherapeutic drugs non-myeloablative pretreatment and human cord blood hematopoietic stem cells transplantation (HSCT) on the spermatogenic function in mice. Methods Thirty Balb/c mice were divided into 3 groups by random digital table method, with 10 mice in each group, which contained control group, Busulfan (BU) combined with Cyclophosphamide (CY) non-myeloablative conditioning (BU+CY) group and HSCT group. In addition to the control group, BU+CY group and HSCT group were pretreated with BU 10 mg/kg and CY 120 mg/kg to induce testicular lesion, and HSCT group was taken human umbilical cord blood HSCT. After HSCT for three weeks, the testicular index was calculated by weighting method. The routine quality analysis of semen and histopathological examination of the testis and epididymis were carried out. Results Compared with the control group, the testicular index, sperm density and sperm viability in BU+CY group were significantly decreased, but the sperm malformation rate was significantly increased (P ＜ 0.01). The graft versus host disease (GVHD) score was higher in HSCT group. The testicular index, sperm density and sperm viability in HSCT group were higher than those in BU+CY group (P ＜ 0.05), while the sperm malformation rate was lower than that of BU+CY group (P ＜ 0.01). The pathological examination found that the testicular volume in BU+CY group was smaller, the seminiferous tubules were obviously impaired, the spermatogenic cells were swelled and arranged in disorder, the seminiferous epithelia was thinned, the number of sperm was significantly reduced in seminiferous tubule and epididymal tubule. Compared with the BU+CY group, the testicular volume in HSCT group was increased, the seminiferous tubule was more integrity, and the number of sperm was increased, but the number of layers seminiferous epithelia cells and spermatocyte were still less. Conclusion BU+CY non-myeloablative conditioning can cause obvious testis injury. Cord blood HSCT can promote a certain extent recovery of testicular spermatogenesis in mild acute GVDH mice.
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