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| Effect of macrophages and pulmonary microvascular endothelial cells on Aspergillus fumigatus |
| SONG Jun HAN Jing SUN Yue SHI Weimin▲ |
| Department of Dermatology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China |
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Abstract Objective To investigate effect of macrophages and pulmonary microvascular endothelial cells (HPMVECs) on Aspergillus fumigatus. Methods In vitro models of Aspergillus fumigatus-treated HPMVECs and Aspergillus fumigatus-treated J774 cells were established, confocal laser scanning microscope was used for detection of co-cultures. Results Aspergillus fumigatus were phagocyted by J774 cells. However, Aspergillus fumigatus were not internalized by HPMVECs, onlu morphology change and integrity compromise were observed on Aspergillus fumigatus-treated HPMVECs. Conclusion J774 cells phagocytes Aspergillus fumigatus. HPMVECs can't internalize Aspergillus fumigatus. Aspergillus fumigatus can cause morphology change and integrity compromise in HPMVECs.
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