中国医药导报
      Home                                                                                    中文
中国医药导报
 
Forthcoming Articles
Current Issue
Archive
Advanced Search
Read Articles
Download Articles
Email Alert
 
Quick Search  
  Adv Search
2018 Vol. 15, No. 10
Published: 2018-04-05
 
 
4 The effect of Pien Tze Huang on the growth of human osteosarcoma tumor stem cell transplantation tumor in nude mice
GUO Wentao ZHANG Yan
Objective To explore the inhibitory effect of Pien Tze Huang on the growth of human osteosarcoma stem cells transplantation tumor of nude mice and its effect on ABCB1 and ABCG2  protein expression. Methods The model of human osteosarcoma tumor stem cell transplantation tumor was established by subcutaneous tumor stem cells in nude mice. According to the random number table method , 48 nude mice were divided into control group, adriamycin group, Pien Tze Huang group, Pien Tze Huang and adriamycin combined group, with 12 mice in each group, The nude mice were executed after four weeks of intervention. The volume and weight of the tumor were measured and the tumor volume and weight inhibition rate were calculated. The expression of ABCB1 and ABCG2 in the tumor was detected by immunohistochemistry (SP) and Western blot. Results Compared with the control group, the volume and weight of tumor were significantly reduced in theadriamycin group, Pien Tze Huang group and combined group (P < 0.05). Compared with Pien Tze Huang group, the combined group growth was significantly inhibited. (P < 0.01). SP and Western blot results showed that the expressions of ABCB1 and ABCG2 in the Pien Tze Huang group and combined group were significantly down-regulated than that in the control group (P ＜ 0.05), and combined group was significantly lower than that in the Pien Tze Huang group (P < 0.05). Conclusion Pien Tze Huang can significantly inhibit the growth of tumor stem cells in human osteosarcoma tumor stem cells, and it can enhance the effect of adriamycin. Which may be related to inhibit the expression of membrane transporter ABCB1 and ABCG2.
2018 Vol. 15 (10): 4-7,12 [Abstract] ( 219 ) HTML (1 KB)  PDF (931 KB)  ( 160 )
8 Effects of flavanomarein on oxidative stress and expression of VEGF and ICAM-1 protein in HUVEC cells induced by high glucose
WANG Lili1 LI Linlin1 ZHANG Nannan1 WANG Ye1 LUO Xin1 TAO Yicun1 MAO Xinmin2
Objective To observe the effects of flavanomarein glycosides of Coreopsis tinctoria Nutt. on oxidative stress and expression of vascular endothelial growth factor (VEGF) and intercellular cell adhesion molecule-1 (ICAM-1) protein in human umbilical vein endothelial cells (HUVEC) induced by high glucose. Methods The cultured HUVEC cells were divided into normal control group (NG group), high glucose model group (HG group) and different concentrations of flavanomarein group (50, 100, 200, 400 μmol/L). The activity of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in supernatant were detected by enzyme-linked immunosorbent assay (ELASA). The expression levels of VEGF and ICAM-1 protein in cells were detected by Western blot method. Results Compared with NG group, the content of MDA in HG group was significantly increased, the levels of SOD, CAT and GSH-Px in HG group were significantly decreased (P ＜ 0.05 or P < 0.01). Compared with HG group, after flavanomarein intervention, the content of MDA in HG group was significantly decreased, CAT, GSH-Px activity increased significantly (P ＜ 0.05). The results of Western blot showed that compared with NG group, the expression of VEGF and ICAM-1 protein in HG group increased (P ＜ 0.05 or P < 0.01). Compared with HG group, after flavanomarein intervention, the expression of VEGF and ICAM-1 protein increased with the increase of concentration followed by reduction (P ＜ 0.05 or P < 0.01). Conclusion Flavanomarein has a protective effect on HUVEC induced by high glucose may be through improving the level of oxidative stress and decreasing the levels of VEGF and ICAM-1.
2018 Vol. 15 (10): 8-12 [Abstract] ( 251 ) HTML (1 KB)  PDF (793 KB)  ( 85 )
13 Study of the inhibition effect of alpinetin on human ovarian cancer cell line OVCAR-8 and its mechanism
ZENG Jing WANG Jing▲ YIN Jinfeng SONG Qi
Objective To explore the effect of alpinetin on human ovarian cancer cell line OVCAR-8 and its mechanism. Methods OVCAR-8 cells were treated with different concentrations of alpinetin (25, 50, 100, 200 and 400 μmol/L, DMSO as control) for different hours (24, 48 and 72 h), then cell viabilities were detected by CCK-8 assays. 3D spheroid assay was used to measure the spheroid formation capacity of OVCAR-8 cell after treated with 50 μmol/L alpinetin (DMSO as control) for 7 days. Besides, wound healing assay was used to measure the migration capacity of OVCAR-8 cell after treated with 100 μmol/L alpinetin (DMSO as control) for 24 h. In addition, Western blot was used to detect the protein levels of STAT3 signaling related and apoptosis related proteins in OVCAR-8 cells after alpinetin treatments (50, 100 and 200 μmol/L, DMSO as control) for 48 h. Results 25-400 μmol/L alpinetin had remarkable inhibiting effects on OVCAR-8 cells and in a dose-and time-dependent manner(all P ＜ 0.05). After 7 days′ treatment, the diameter of spheroid in 50 μmol/L alpinetin group was significantly shorter than DMSO control group (P ＜ 0.05). Besides, the width of wound in 100 μmol/L alpinetin group was significantly longer than DMSO control group after  treatment for 24 h (P ＜ 0.05). In addition, the protein levels of Bcl-2, p-STAT3, c-myc and survivin were decreased in 50, 100 and 200 μmol/L group of OVCAR-8 cells than DMSO control group after 48 exposure. On the contrary, the protein level of Bax, cleaved-caspase-3 and -9 was increased (all P ＜ 0.05), and there were no significantly changes in STAT3 levels (P ＞ 0.05). Conclusion Alpinetin can inhibit the proliferation and migration of OVCAR-8 cells,its mechanism may be related to the inhibition of STAT3 signaling pathway and the activation of mitochondrial apoptosis signaling．
2018 Vol. 15 (10): 13-17 [Abstract] ( 249 ) HTML (1 KB)  PDF (904 KB)  ( 77 )
18 Effect of rehabilitation training on cognitive dysfunction in type 2 diabetic rats
TANG Mingwei MEI Xi WANG Fengbo▲
Objective To observe the effect of rehabilitation training on the improvement of cognitive impairment in type 2 diabetic rats. Methods 36 adult Wistar rats were divided into type 2 diabetes model group (n=24) and blank control group (n=12) by the random number table method. Preparation of type 2 diabetic rat model with high fat and high sugar diet plus intraperitoneal injection of streptozotocin. After the rat models were successfully prepared, the rats of type 2 diabetes group were randomly divided into non-intervention group (n=12) and rehabilitation training group (n=12). The rehabilitation training group began to intervene on the following day after the success of the model preparation, 1 times a day. The cognitive function (space learning and memory ability) of all rats were tested by the Morris water maze in the second, fourth, sixth week of the rehabilitation training group. Results Compared with the blank control group, the escape latency in the non-intervention group was significantly prolonged (P < 0.01), and gradually increased, and the number of crossing platform was also significantly reduced (P < 0.01). Compared with non-intervention group, the escape latency of the rehabilitation training group was significantly improved (P < 0.01), and the number of crossing platform increased significantly (P < 0.01). There were no significant difference between the sixth week rehabilitation training group and the blank control group in the escape latency and the number of crossing platforms (P > 0.05). Conclusion Rehabilitation training can significantly improve the spatial learning and memory ability of type 2 diabetes rats. With the increase of training time, the improvement effect is more significant, which is conducive to the recovery of cognitive dysfunction in diabetic rats.
2018 Vol. 15 (10): 18-21 [Abstract] ( 762 ) HTML (1 KB)  PDF (513 KB)  ( 99 )
22 Mechanism of HMGB1/NF-κB signal pathway affecting cerebral development of offspring mice after exposure to PM2.5 during pregnancy
LI Wenyan1 GUO Ya'nan1 ZHENG Xinrui1 ZHANG Hongxia1 YU Li1 JIANG Hong2
Objective To investigate the mechanism of HMGB1/NF-κB signal pathway affecting cerebral development of offspring after exposure to PM2.5 during pregnancy. Methods Fourty pregnant Kunming mice were randomly divided into 5 groups, 8 rats in each group. PM2.5 low-dose group, medium-dose group and high-dose group were instilled 30 μL PM2.5 suspension of 0.25920, 1.56695, 3.45600 μg/μL respectively by improved technique for rapid intratracheal instillation. The control group was instilled PBS of the same volume. The blank group received no treatments. Mice were instilled tracheally once every 3 days for 7 times during pregnancy. ELISA was used to measure the levels of TNF-α, IFN-γ, HMGB1 and NF-κB p65 in the cerebral cortex of offspring mice. Western blot and RT-PCR were used to detect the protein and mRNA expression of HMGB1 and NF-κB p65. Results ELISA showed that compared with the control group, the levels of IFN-γ, NF-κB p65 and HMGB1 in the high and middle dose groups were significantly lower (P < 0.05); high-dose group TNF-α increased (P < 0.05). Western blot showed that HMGB1 decreased and NF-κB p65 increased in the high and middle dose groups compared with the control group (P < 0.05). PCR showed HMGB1 mRNA unchanged; high-dose group NF-κB p65 mRNA was higher than the control group (P < 0.05). Conclusion PM2.5 exposure during pregnancy may cause inflammation in the cerebral cortex of offspring mice, and HMGB1/NF-κB pathway may play an important role.
2018 Vol. 15 (10): 22-24,28 [Abstract] ( 270 ) HTML (1 KB)  PDF (628 KB)  ( 66 )
25 Study on the effect of Fuzheng Yiliu Decoction on invasion and metastasis of hepatocellular carcinoma cells
PENG Jianxin HE Junming CAI Bingqing CHEN Guihao LIU Ming
Objective To investigate the effect of Fuzheng Yiliu Decoction on invasion and metastasis of hepatocellular carcinoma cells (HCC). Methods Hepatocellular carcinoma cells were treated by Fuzheng Yiliu Decoction. MTT assay was used to detect the proliferation of hepatocellular carcinoma cells. The migration ability of HCC cells was detected by scratch test, while the invasion ability of HCC cells was detected by Transwell assay. Results MTT results showed that in HCC lines HepG2, MHC97-H, the proliferation ability of 400 mg/mL Fuzheng Yiliu Decoction group was significantly lower than the control group (P < 0.05). Scratch test demonstrated that in liver cancer cell MHC97-H, the migration rate of liver cancer cells in Fuzheng Yiliu Decoction 400 mg/mL group was significantly lower than that in the control group (P < 0.05). Transwell experiment proved that in liver cancer cell MHC97-H, the number of invading hepatoma cells in the 400 mg/mL Fuzheng Yiliu Decoction group was significantly lower than that in the control group (P < 0.05). Conclusion Fuzheng Yiliu Decoction can inhibit the proliferation, invasion and metastasis of HCC.
2018 Vol. 15 (10): 25-28 [Abstract] ( 200 ) HTML (1 KB)  PDF (687 KB)  ( 137 )
29 Study on the quality standard and preliminary pharmacodynamics of Freeze-dried Toad Skin Gel 
LU Hongyan1 HUANG Shengwu2▲
Objective To establish the quality standard and preliminary pharmacodynamic study of Freeze-dried Toad Skin Gel. Methods TLC was used for the qualitative identification of Cinobufagin and Resibufogenin, HPLC was adopted to determine content of Cinobufagin and Resibufogenin. Chromatographic column: kromasil C18 (4.6 mm×250 mm, 5 μm); flowing phase: acetonitrile (A)- water (B) (48∶52); flow rate: 1 mL/min; UV detection wavelength: 296 nm; column temperature: 30℃; the volume: 10 μL. S180 ascites solid tumor mice were used to study the pharmacodynamics of inhibition of tumor growth. Results The fluorescence spots of the same color were shown in the corresponding position of the Silica gel G thin layer, and the spot size was moderate and the separation degree was good. Cinobufagin and Resibufogenin showed good linearity between the peak area and the concentration in the range of 0.056-4.48 μg/mL (r=0.9987) and 0.256-10.24 μg/mL (0.9996), respectively. The sample recovery rate(n=6) was 100.27%, 101.68%, RSD was 1.56% and 1.67%. The Preliminary pharmacodynamics study showed that all groups of Freeze-dried Toad Skin Gel had significant anti-tumor effect, and the high-dose group had the best effect, with the inhibitory rate of 48.03%. Conclusion The quality standard of Freeze-dried Toad Skin Gel is established, and the preliminary pharmacodynamics shows that it has a significant anti-tumor effect.
2018 Vol. 15 (10): 29-33 [Abstract] ( 252 ) HTML (1 KB)  PDF (585 KB)  ( 86 )
34 Study of Pharmacokinetics of Cefoperazone-Sulbactam in patients with ICU sepsis
YI Lingao CHEN Xiaohai HE Guoxin CHEN Jie LIN Jie
Objective To investigate the pharmacokinetics of Cefoperazone/Sulbactam in intensive care unit patients with sepsis. Methods The patients with sepsis in intensive care unit of Ruian people′s Hospital of Zhejiang Province from January to September 2017 who was administered 2.0 g Cefoperazone/Sulbactam for the first time were inrolled. Serial serum samples were collected before the first antibiotic dose and at 0, 0.25, 0.5, 1, 2, 3, 4, 6, 8 h after Cefoperazone/Sulbactam was administered. Then the serum was separated and the concentration was determined by HPLC. Results Cefoperazone: half-life (t1/2): (1.575±0.325) h, clearance rate（CL）: (0.095±0.023) L/(h·kg), apparent volume of distribution (Vd): (0.215±0.037) L/kg, peak concentration (Cmax): (83.633±19.324) mg/L, area under the curve AUC (0→∞): (190.213± 42.625) h·mg/L; Sulbactam: t1/2: (1.049±0.263) h, CL: (0.281±0.065) L/(h·kg), Vd: (0.424±0.103) L/kg, Cmax: (42.526±11.210) mg/L, AUC(0→∞): (64.394±15.435) h·mg/L. Conclusion The apparent volume of distribution of Cefoperazone in intensive care unit patients with sepsis is slightly larger than that reported in the literature, the peak concentration is slightly lower, and the half-life is shorter, while the change of Sulbactam is relatively small. Therefore, the dosage of Cefoperazone should be improved and the dosing interval should be shortened in those patients.
2018 Vol. 15 (10): 34-37,41 [Abstract] ( 270 ) HTML (1 KB)  PDF (1044 KB)  ( 147 )
38 Research progress of traditional Chinese medicine in regulating the autophagy of cardiovascular diseases
XU Jiwen1 LI Jinxia2 ZHANG Huamin3▲
Autophagy is a highly conservative process in which eukaryotic cells maintain homeostasis and it plays an regulatory role in the occurrence and development of cardiovascular disease (CVD) such as myocardial ischemia and reperfusion injury and atherosclerosis, which is a new target for CVD. The theory of traditional Chinese medicine, such as reestablishment to yin-yang equilibrium, phlegm stasis due to deficiency of qi, liver controlling conveyance and dispersion are closely related to autophagy. Many literatures show that the protective effect of TCM on cardiovascular disease is achieved through autophagy. In this paper, the autophagy in CVD is discussed, and a brief overview of the research progress in the regulation of CVD autophagy in Chinese medicine is presented.
2018 Vol. 15 (10): 38-41 [Abstract] ( 217 ) HTML (1 KB)  PDF (489 KB)  ( 114 )
42 Research progress on SIRT5 as deacylase
ZHENG Chunlei1,2 LU Wenqing1,2 CHE Xiaofang1,2 LIU Yunpeng1,2▲
Sirtuin 5, also called SIRT5, is a member of Sirtuins family. Besides its deacetylation activity, SIRT5 also has strong desuccinylation, demalonylation and deglutarylation activities. Therefore, SIRT5 has been attracted much attention in recent years. Currently, about more than tenproteins were identified as substrates of SIRT5. SIRT5 is involved in the regulation of glucose oxidation, ROS metabolism and fatty acid oxidation, ammonia removal, detoxification, anti apoptosis, inflammation and so on, by playing the same enzymatic activity on different substrates, or playing different enzymatic activity on the same substrate. The abnormal expression of SIRT5 is closely related to the occurrence and development of tumor, though its function is not clarified yet. SIRT5 can play the role of oncogene to promote tumor proliferation, metastasis, drug resistance and metabolic reprogramming. On the other hand, SIRT5 also play the role of tumor suppressor gene to inhibit tumor cell growth and apoptosis. In addition, SIRT5 abnormalities are also closely related to hypertrophic cardiomyopathy, myocardial infarction and other cardiovascular diseases, such as Parkinson′s disease and Alzheimer′s disease. In order to have a good comprehensive on the research progress of SIRT5, this paper reviews the structure and regulation, enzyme activity and biological function of SIRT5, and the relationship between SIRT5 and desease in details here.
2018 Vol. 15 (10): 42-45 [Abstract] ( 395 ) HTML (1 KB)  PDF (491 KB)  ( 313 )
46 Strategies for immunotherapeutic targeting of regulatory T cells in tumor microenvironment
LIU Jialing1* WANG Shimin1* ZHUO Zhaozhen2 YUAN Jun3
Regulatory T cell (Treg) is a group of lymphocytes with negetive regulation of inhibiting tumor-associated antigen T cell-mediated immune responses, targeted agents may influence tumor immunotherapy and vaccine activation. Systemic removal of CD25+CD4+ T cells perhaps elicit tumor growth in rodents, but also increasing the severity of the autoimmune diseases as peripheral self-tolerance is maintained by Treg cells. According to sources of Regulatory T cells, it can be classfied as two population of nTreg and iTreg cells. Regulatory T cells are phenotypically diversity and functionally heterogeneous in different types of tumor and tissues, whereas conferring the same marker of CD4, CD25 and foxp3. Because of its tumor heterogeneity and organ heterogeneity, therefore, the strategy of targeting Treg cells in tumor immunotherapy is different. On the other hand, removal Treg cell strategies is different in tumor immunotherapy, it can remove regulatory T cells (Treg) or dampen the function of Treg cells through limit its immunosuppression protein, such as GITR, T-bet, Neoropilin-1, CTLA-4, IDO or PD-1. The autoimmunities can be prevented by inoculating OVA vaccine when removing Tregs .Ipilimumab, which has recently been FDA-approved for therapy patients with advanced melanoma mechnism is targeting CTLA- 4 in Treg cells. In this paper, we review the phenotype, heterogeneity and tumor therapy strategies of Treg treatment in order to avoid immune disorders in the future.
2018 Vol. 15 (10): 46-50 [Abstract] ( 359 ) HTML (1 KB)  PDF (503 KB)  ( 186 )
51 The interaction of Clopidogrel with SSRIs mediated by CYP2C19
FAN Youmin1 MA Xiuping2 CHEN Songjie1 GENG Deqin2▲
Along with the general rising of the incidence of post-stroke depression, selective serotonin reuptake inhibitors (SSRI) and dual anti-platelet therapy are widely used for patients with post-stroke depression in clinic. However, both SSRI and Clopidogrel are metabolized by hepatocyte pigment P450 isoenzyme system, some meta-analysis and small sample researches indicate that SSRI can reduce the anti-platelet effects of Clopidogrel by inhibiting the activity of hepatocyte pigment P450 isoenzyme CYP2C19. According to the different chemical structures and pharmacokinetic characteristics of SSRIs, the degrees of suppression of SSRI are not alike. This article summaries the drug metabolism pathway, mechanism and clinical research progress of this two drugs.
2018 Vol. 15 (10): 51-54,67 [Abstract] ( 249 ) HTML (1 KB)  PDF (1073 KB)  ( 117 )
55 Analysis of pathogen distribution and drug resistance of urinary tract infection in senile diabetics
HAN Shuxia NI Wei
Objective To discuss the pathogen distribution and drug resistance of urinary tract infection in senile diabetics, in order to offer evidence for reasonable use of antibiotics. Methods The urine culture and drug sensitivity results were analyzed among senile diabetics with urinary tract infection from 2014 to 2016 in Hubei Provincial Hospital of Traditional Chinese Medicine. Results Among the 331 strains of pathogenic bacteria, the first three were Escherichia coli (194 strains, 58.6%), Enterococcus faecalis (30 strains, 9.1%) and Pseudomonas aeruginosa (27 strains, 8.2%). The detection rate of ESBLS-producing strains was 55.2%（107/194) of Escherichia coli, which of Klebsiella pneumoniae was 66.7% (16/24). Resistant rate of Gram-negative bacteria on Amikacin and Imipenem was low, but the rates of that on Ampicillin/Sulbatam, Levofloxacin, Ciprofloxacin and Ceftriaxone were all greater than 50%. The resistant rate of Enterococcus faecalis and Enterococcus faecium were greater than 50% to Clindamycin, high concentration Gentamycin and high concentration Streptomycin, but were highly sensitive to Vancomycin and Linezolid. Conclusion Urinary tract infection in senile diabetics is mainly caused by Escherichia coli and Enterococcus, which are highly resistant to routine antibiotics. Clinicians should attach great importance to drug sensitivity results and select antibiotics according to the result of urine culture and drug sensitive test.
2018 Vol. 15 (10): 55-57,71 [Abstract] ( 233 ) HTML (1 KB)  PDF (657 KB)  ( 90 )
58 Application effect of traditional Chinese medicine lung rehabilitation model in stable period of chronic obstructive pulmonary disease
TIAN Yuan1 LI Guoqin2 XU Huilian1 LI Chunyu1 Quan Jianfeng1 LIU Yushan1 CHU Chen1 LI Hui1
Objective To observe the effect of traditional Chinese medicine (TCM) lung rehabilitation therapy and drug treatment on patients with chronic obstructive pulmonary disease (COPD). Methods From November 2015 to November 2016, 94 COPD stable patients were selected, by random number table, they were divided into rehabilitation group (47 cases) and control group (47 cases). The control group was treated by simple drug therapy, and the rehabilitation group was treated with traditional Chinese medicine pulmonary rehabilitation therapy on the basis of drug treatment. The data were collected before treatment, 1 month after treatment, 3 months after treatment, 6 months after treatment, and the therapeutic effect was analyzed and evaluated. The indexes included St Georges Respiratory Questionnaire (SGRQ); a second forced expiratory volume (FEV1), forced expiratory volume in one second percentage of predicted value (FEV1%pred), forced vital capacity (FVC), forced expiratory volume in one second forced vital capacity ratio (FEV1/FVC), per minute ventilation volume (MVV); 6 minutes walk test and BODE score. And the effect of treatment was analyzed and evaluated. Results The number of admissions in two groups after the treatment were less than before the treatment, and that in rehabilitation group was less than control group, the differences were statistically significant (P < 0.05). After the treatment, the SGRQ scores of the rehabilitation group was lower than control group (P < 0.05); MVV detection in the rehabilitation group after 1 months treatment was better than control group at the same period (P < 0.05); FEV1%pred, FVC and MVV detection after 3 months treatment were better than control group at the same time (P < 0.05); FEV1, FEV1%pred, FVC, MVV and FEV1/FVC detection after 6 months treatment were better than control group at the same time (P < 0.05); 6 minutes walking test results in rehabilitation group after treatment were better than the control group (P < 0.05); the BODE index scores of rehabilitation group after treatment were lower than control group (P < 0.05). Conclusion TCM pulmonary rehabilitation model can improve COPD patients daily living and quality of life, improve exercise tolerance and respiratory muscle function, thereby improve the lung function that drugs is difficult to reverse.
2018 Vol. 15 (10): 58-62 [Abstract] ( 766 ) HTML (1 KB)  PDF (694 KB)  ( 138 )
63 Clinical effect of thoracoscopic surgery for patients with pulmonary aspergilloma and the influence on the postoperative stress reaction
CAI Renzhong CHEN Xianshan CHEN Fengxia
Objective To discuss the possibility and safety of video-assisted thoracoscope surgery (VATS) for pulmonary aspergilloma and the postoperative stress reaction. Methods The clinical data of 66 patients with pulmonary aspergilloma in People′s Hospital of Hainan Province from February 2011 to September 2016 were retrospectively analyzed. The patients were divided into two groups according to their operation method including a conventional thoracotomy surgery group (open group, n = 36) and a video-assisted thoracoscope pneumonectomy group (VATS group, n = 30). The clinical data, complications, NPS and stress reaction-related indicators of the two groups were observed after surgery. Results The operations of all patients were performed successfully. The VATS group was significantly lower than the open group in the operation time, bleeding, total postoperative drainage, the 7th day pain score and the 30th day pain score (P < 0.05). There were no statistical differences in the time of hospitalization, the 1st day pain score and postoperation complication (P > 0.05). In stress response indexes (cortisol, prostaglandin E2 and P substance), the postoperative levels of 1st day and 7th day in the VATS group were significantly lower than those in the open group (P < 0.05). In inflammatory reaction indexes (C-reactive protein, interleukin 6 and tumor necrosis factor-α), the postoperative levels of 1st day and 7th day in the VATS group were significantly lower than those in the open group (P < 0.05). Conclusion VATS for pulmonary aspergilloma, if applicable, may be reliable therapeutic effect, and less impact on the postoperative stress reaction. It is worthy of clinical application.
2018 Vol. 15 (10): 63-67 [Abstract] ( 219 ) HTML (1 KB)  PDF (761 KB)  ( 90 )
68 Analysis of the characteristics of lipid metabolism and stone composition in patients with cholecystolithiasis of Uyghur people in Kashgar region
Alimujiang·ABUDUREYIMU1▲ Mushajiang·ABUDUKELIMU1 Sipireguli·ABUDUKADI2▲ Nueraili·KADI3
Objective To investigate the characteristics of lipid metabolism and stone composition in the patients with simple cholecystolithiasis of Uyghur people in Kashgar region. Methods One hundred and eighty-eight patients with simple cholecystolithiasis treated in the Second People′s Hospital of Kashgar from January 2015 to December 2016 were selected as the patient group, and 172 healthy examined people were selected as the normal control group. The blood lipid levels and stone composition of each group were checked. The differences on plasma lipid levels and gallstone compositions in different gender of the patient group were analyzed. Results The contents of plasma CHOL, TG and APO-B in the patient group were significantly higher than those in the normal control group (P < 0.05). The contents of plasma HDL-C and APO-A1 levels were lower in the female patients than in the men patients (P < 0.05) in the patients group, while cholelithiasis was mainly composed of cholesterol stone (56.13%). Conclusion Abnormal lipid metabolism is one of the causes of cholecystolithiasis, especially in female.
2018 Vol. 15 (10): 68-71 [Abstract] ( 269 ) HTML (1 KB)  PDF (645 KB)  ( 87 )
72 Correlation between Cytochrome P450 Enzyme 2E1 expression and Sevoflurane MACBAR in patients with passive smoking
XU Yongsheng1 LU Jingang2 XIA Zunping1
Objective To detect the change of CYP2E1 level in passive smoking patients and analyze its correlation with Sevoflurane MACBAR value. Methods From May 2017 to October 2017, in the Fifth Hospital of Harbin City, 90 patients with femoral head necrosis underwent artificial joint replacement under general anesthesia were selected. By inquiring the patients′ own tobacco exposure and combining with the content of cotinine, the patients were divided into passive smoking group (group A, 49 cases), non-smoking group (group B, 41 cases). Sevoflurane MACBAR was measured by single stimulus. HR, MAP before induction of anesthesia, 3 min and 1 min before cutting, 1 min and 3 min after cutting were recored. On the day before surgery, 5 mL of patients′ morning venous blood was gotten, centrifuged and all patients′ sera was collected for the detection of Cotinine and CYP2E1 content. Results In group A, the increase of average MAP value at 1 min and 3 min after skin incision was greater than that of group B, the difference was statistically significant (P < 0.05). The contents of Sevoflurane MACBAR, CYP2E1 and cotinine in group A were significantly higher than those in group B (P < 0.05). The CYP2E1 level in group A was positively correlated with MACBAR (r = 0.45, P < 0.05). Conclusion The level of CYP2E1 in passive smoking is correlated with MACBAR of Sevoflurane, it suggests that CYP2E1 in serum of passive smokers may affect the Sevoflurane MACBAR value.
2018 Vol. 15 (10): 72-74,90 [Abstract] ( 207 ) HTML (1 KB)  PDF (940 KB)  ( 97 )
75 Application of pepsinogen, thymidine kinase 1 joint detection in the screening for gastric cancer
LI Weibing1 WANG Kuiming1 HOU Weiwei1 YU Haizhong1 YUAN Jianfen1 YAO Hui2 XIAO Chunhong3▲
Objective To investigate the value of pepsinogen (PG) and thymidine kinase 1 (TK1) combined detection for gastric cancer screening. Methods Two hundred and fifty-eight cases were included in the research from February 2016 to June 2017, who were stomach cancer (n=178) [including early gastric cancer (n=46), progress gastric cancer (n=60)], atrophic gastritis (n=72), and healthy individuals (n=80) as control respectively. Blood PG and TK1 were assayed with enzyme linked immonosorbent assay (ELISA) and Western blot-enayme immunoassay chemilumiescence commercial kit. Results PGⅠand PGⅠ/Ⅱ(PGR) of gastric cancer were lower than those of atrophic gastritis and normal control (P < 0.05), and which of advanced gastric cancer were lower than those of early gastric cancer (P < 0.05). TK1 of gastric cancer was higher than that of atrophic gastritis and normal control patients (P < 0.05), which had nothing to do with the staging of gastric cancer (P > 0.05). Sensitivity and specificity for PG and TK1 were 52.1%, 81.9% and 47.2%, 86.1% respectively. Sensitivity and specificity for PG and TK1 combined assay were 81.1% and 79.2%, which were significantly higher than those of individual assay (P < 0.05). Conclusion This study shows that PG and TK1 combined detection has excellent value for gastric cancer screening.
2018 Vol. 15 (10): 75-78 [Abstract] ( 713 ) HTML (1 KB)  PDF (687 KB)  ( 120 )
79 Curative effect of single-port and double-port thoracoscopic pleural fiberboard treating encapsulated pleural effusion
LI Pengfei1 SHANG Na2 WANG Yanjun1
Objective To explore the effect of single-port and double-port thoracoscopic fibrostectomy treating of encapsulated pleural effusion. Methods From April 2010 to April 2015, in Yan′an People′s Hospital, the clinical data of 135 patients with encapsulated pleural effusion were retrospectively analyzed, according to the operation method, they were divided into three groups, single-port thoracoscopic fibrostectomy group (single-port group, 45 cases), double-port thoracoscopic fibrostectomy group (double-port group, 53 cases), open-thoracic operation (tradition group, 37 cases). The clinical outcomes and complications of three groups were analyzed and compared. Results The clinical indicators of three groups were compared, single-port group patients had the shortest incision length (F = 8585.958, P = 8585.958), the least intraoperative blood (F = 686.322, P = 0.000), the shortest hospital stay (F = 406.947, P = 0.000), the shortest chest tube drainage (F = 95.563, P = 0.000), the highest score of incision satisfaction score (F = 68.241, P = 0.000), the lowest VAS score of 6 h postoperative pain (F = 90.219, P = 0.000) and he lowest VAS score of 24 h postoperative pain (F = 263.125, P = 0.000). Meanwhile, the incidence of atelectasis (χ2 = 17.667, P = 0.000) and incisional infection rate (χ2 = 13.841, P = 0.001) of the single-port group were significantly lower than the double-port group and traditional group. The postoperative incision pictures showed that two weeks recovery state after the operation of the single-port group were better than the double-port group and the traditional group. Conclusion Compared with double-port thoracoscopy and traditional open-thoracic surgery, single-port thoracoscopic surgery has certain advantages, which can obviously improve the curative effect to treat encapsulated pleural effusion.
2018 Vol. 15 (10): 79-82 [Abstract] ( 176 ) HTML (1 KB)  PDF (721 KB)  ( 132 )
83 Application effect of damage control theory in elderly patients with hip fracture
YE Zheng CHEN Jian
Objective To investigate the effect of damage control theory on the cure rate and complication rate of elderly patients with hip fracture. Methods From January 2015 to May 2017, 145 elderly patients with hip fracture were selected and randomly divided into two groups from Putuo People′s Hospital Affiliated to Tongji University. The control group was received conventional treatment and the treatment group was received the injury control theory on the basis of conventional treatment. The efficacy, quality of life, self-rating anxiety scale (SAS) and self-rating depression scale (SDS), Oxford disability scale (OHS scale) and complication were compared. Results The excellent and good rate of the treatment group was significantly higher than the control group, the difference was statistically significant (P < 0.05). The scores of quality of life of the treatment group were higher than the control group, the differences were statistically significant (P < 0.05). SAS and SDS scores of the two groups after treatment were significantly lower than before treatment, and the treatment group scores were lower than the control group, the differences were statistically significant (P < 0.05). There were no significant differences between the two groups in the OHS scores before treatment, and the scores of the treatment group after treatment were lower than those of the control group (P < 0.05). The incidence of complications in the treatment group was lower than that in the control group, the difference was statistically significant (P < 0.05). Conclusion Adopting the theory of injury control in elderly patients with hip fracture can speed up the healing time, reduce the pain and the incidence of complications, which has high clinical value.
2018 Vol. 15 (10): 83-86 [Abstract] ( 201 ) HTML (1 KB)  PDF (644 KB)  ( 85 )
87 Effects of Propofol and Dexmedetomidine on patients undergoing intraperitoneal stress by intraperitoneal anesthesia for abdominal surgery
LIANG Lu1 SU Baoxiong2 QU Xiaoxiao3

Objective To investigate the effect of Propofol combined with Dexmedetomidine on stress response in patients undergoing peritoneal surgery under anesthesia. Methods One hundred and six patients admitted to the Affiliated Hospital of Yan'an University undergoing abdominal surgery from February 2015 to February 2017 were divided into two groups according to random number table. In the control group, Propofol was used to maintain anesthesia. In the observation group, Propofol and Dexmedetomidine were used for anesthesia maintenance. The serum levels of A-Ⅱ, NE, COR and E at T0, skin incision (T1), the beginning of surgery 0.5 hours (T2) and before the tube (T3) were compared between two groups. The changes of GSH-PX, LPO and Cu-Zn SOD in both groups before and after extubation were observed. The occurrence of adverse reactions within 24 h after surgery were record in two groups. Results Compared with T0, the serum levels of A-Ⅱ, NE, COR and E increased significantly during T1 to T3(P < 0.05), and the above indexes in the observation group were significantly lower than those in the control group at three time points from T1 to T3(P < 0.05). After extubation, the levels of GSH-PX, Cu-Zn SOD were significantly decreased(P < 0.05), and LPO levels were significantly increased in the two groups (P < 0.05). The levels of GSH-PX, Cu-Zn SOD in the observation group were significantly higher than those in the control group (P < 0.05), and the LPO level was significantly lower than that of the control group (P < 0.05). The incidence of adverse reactions in the observation group was only 5.66% at 24 h after operation, which was significantly lower than that in the control group (18.87%) (P < 0.05). Conclusion Propofol and Dexmedetomidine combined application of anesthesia in patients undergoing abdominal surgery, can effectively inhibit the stress response during surgery, ease intraoperative oxidative damage, has fewer adverse reactions, and has high safety.
2018 Vol. 15 (10): 87-90 [Abstract] ( 201 ) HTML (1 KB)  PDF (451 KB)  ( 178 )
91 The application of target controlled infusion with Propofol by Marsh model versus Schnider model during gynecological laparoscopic surgery 
WEI Hong FENG Jiehua Tu Hankun LI Chaoyang▲ WU Dong LUO Xiaoming
Objective To evaluate the applicability of target controlled infusion (TCI) with Propofol by March model and Schnider model during gynecological laparoscopic surgery. Methods One hundred unpremedicated patients (18 to 55 years, ASA Ⅰor Ⅱ ) undergoing gynecologic laparoscopy in Shenzhen Nanshan People′s Hospital from Janurary to June 2016 were randomized into two groups (n = 25 each ) using a random number table ：group M and group S. Propofol was administered by TCI, with respectively March model and Schnider model in two groups, and combined with Remifentanil. The age, weight, anesthetic time, operative time, time for loss of consciousness, the corresponding amount of Propofol and effect site concentration of Propofol (Ce) at loss of consciousness (LOC) and Ce at recovery of consciousness(ROC), time from discontinuation to analepsia, time from discontinuation to extubation, heart rate (HR), mean arterial blood pressure (MAP) and bispectral index (BIS) were monitored and recorded before induction (T0), loss of consciousness (T1), before intubating (T2), after intubating (T3), skin incision (T4), consciousness (T5), extubation (T6), the total amount and unit time amount of Propofol. Intraoperative awareness was evaluated at 24 hours postoperation. Results There was no significant different in the time of lose consciousness and the amount of Propofol between group M and S (P > 0.05). but The total amount and unit time amount of Propofol in group S were more than that in group M (P < 0.05). The time from discontinuation to analepsia and the time from discontinuation to extubation of group S were both longer than group M (P < 0.05). Compared with T0, there were no significant different in HR, MAP at T1, T2, T3, T4, T5 and T6 in both groups(P > 0.05). Compared with group M, there were no significant different in HR, MAP at T0-6 in group S(P > 0.05). There were no significant different in BIS at T0, T2, T3 between the two groups, but at T1, T4, T5, T6 BIS in group S were lower than in group M (P < 0.05). No intraoperative awareness happended. In group M Ce at LOC was positively correlated to Ce at ROC (P < 0.05). Conclusion TCI with Propofol during gynecological laparoscopic surgery, Marsh model was better than Schnider model.
2018 Vol. 15 (10): 91-94 [Abstract] ( 270 ) HTML (1 KB)  PDF (452 KB)  ( 122 )
95 Effects of Dexmedetomidine combined with Lidocaine on oxidative stress and inflammatory response in laparoscopic hysterectomy patients
ZHANG Nu XU Liansheng
Objective To expiore the effects of Dexmedetomidine combined with Lidocaine on oxidative stress and inflammatory response in patients with laparoscopic total hysterectomy. Methods One hundred and twenty patients with laparoscopic total hysterectomy in gynaecology of the First People′s Hospital of Yibin from September 2014 to October 2017 were selected as the subjects. According to the random digital table method,120 patients were divided into group A, B, C and control group, 30 cases in each group. Group A was treated with dexmedetomidin combined with Lidocaine by intravenous pump. Group B was treated with Dexmedetomidine by intravenous pump. Group C was treated with Lidocaine by intravenous pump. The control group treated with normal saline by intravenous pump. The inflammatory factors (IL-6, TNF-α) and oxidative stress indexes (H2O2, MDA and TAS) were monitored before administration (T1), at the end of operation (T2), 2 h after operation (T3) and 24 h after operation (T4). The incidence of postoperative adverse reactions was compared between the four groups. Results At T2, T3 and T4, levels of serum H2O2 and MDA, group A < group B< group C< control group, the difference between groups was statistically significant (P < 0.05). Levels of serum IL-6, TNF-α in group A < group B< group C< control group, and the TAS leves, group A> group B> group C> control group, the difference between groups was statistically significant (P < 0.05). The incidence of nausea and vomiting in group A was lower than that in group B and control group, the difference was statistically significant (P < 0.05). The incidence of somnolence and bradycardia in group A and B was higher than that in group C and control group, the difference was statistically significant (P < 0.05). Conclusion Dexmedetomidine combined with Lidocaine can alleviate the oxidative stress response and inflammatory response in patients with laparoscopic total hysterectomy. However, the incidence of somnolence and bradycardia increased after operation.
2018 Vol. 15 (10): 95-98,134 [Abstract] ( 260 ) HTML (1 KB)  PDF (754 KB)  ( 110 )
99 Application of aromatic drugs in the diagnosis and treatment of allergic rhinitis
ZHANG Yu1 LI Jingyi2 ZHUO Ga3 LI Fuyao1 DAI Ning1 REN Qingjia4 LI Feng1
The number of patients with allergic diseases will grow rapidly with the prosperity of the economy, in which allergic rhinitis can develop into allergic asthma, induce anaphylactic shock, and then endanger life. Therefore, effective treatment of allergic rhinitis and blocking the process can prevent the occurrence of allergic asthma and many other diseases. At present, drug treatment in western medicine is mainly symptomatic treatment, but the recrudescence of allergic rhinitis and the process of disease can not be effectively prevented; immunotherapy for allergies can be used as a cure, but there are many allergies that have not developed immune agents and the variety of allergies is too numerous to enumerate. Therefore, the Chinese medicine treatment of allergic rhinitis is especially worthy of research. Starting from the etiology and pathogenesis of allergic rhinitis, utilize aromatic drugs in natural drugs to reduce phlegm dampness, regulate qi and activate blood, open the orifices and spread the collaterals, clarify the mechanism of the treatment of allergic rhinitis, and then carry out the study on the effective components of medicinal herbs in the treatment of allergic rhinitis, action target and therapeutic pathway, and lay the foundation for the development and research of new drugs and new dosage forms for allergic rhinitis.
2018 Vol. 15 (10): 99-101 [Abstract] ( 217 ) HTML (1 KB)  PDF (679 KB)  ( 135 )
102 Analysis of Shi Jinmo's experience in the application of fermented traditional Chinese medicine
LIU Tongting
This paper analyzes the use of fermented Chinese medicine in 38 cases of Shi Jinmo's medical records, and investigates the usage and concerted application characteristics of the fermented Chinese medicine. As a processing method of traditional Chinese medicine, fermentation can improve the extraction ratio of effective constituents, come into new compound and optimize decomposition, promote drug absorption and utilization, and it also can decrease toxicity and increase efficacy. Semen Sojae Praeparatum, Rhizoma Pinelliae Fermentata, Fujian medicated leaven, Chinese eaglewood leaven, saute red yeast rice, Xiatian leaven 6 kinds of fermented traditional Chinese medicine are widely used in Shi Jinmo′s medical records, which are made couplet medicines with different Chinese herbal medicines to treat respiratory and digestive, cardiovascular and other types of cases positively, the curative effect is definite, and it is worthy of inheritance and development.
2018 Vol. 15 (10): 102-105 [Abstract] ( 156 ) HTML (1 KB)  PDF (647 KB)  ( 120 )
106 Clinical effect of Yougui Pills combined with ginger moxibustion in the treatment of knee osteoarthritis with syndrome of yang deficiency and coagulated cold
SHAO Jieqi ZENG Zhijiang YANG Min LU Xiangran ZHONG Qiusheng
Objective To explore the clinical curative effect of Yougui Pills combined with ginger moxibustion in the treatment of knee osteoarthritis with syndrome of yang deficiency and coagulated cold. Methods One hundred and ten cases of knee osteoarthritis patients with syndrome of yang deficiency and coagulated cold treated in the Third People's Hospital of Dongguan City form June 2013 to June 2017 were selected and randomly divided into control group and experimental group by random number table method, with 55 cases in each group. The control group was given oral Yougui Pills, the experimental group was given Yougui Pills combined with ginger moxibustion. The serum inflammatory activity indicators, serum immunological indices, and the scores of Lequesne index and yang deficiency syndrome of the two groups before and after treatment were compared. Results After treatment, the serum inflammatory activity indicators of erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and 28 human body joint function assessment (DAS28), the serum immunological indices of interleukin 1 (IL-1), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α) and the scores of Lequesne index and yang deficiency syndrome in the two groups were significantly lower than those before treatment (P ＜ 0.05), and the serum inflammatory activity indicators, serum immunological indices, the scores of Lequesne index and yang deficiency syndrome in the experimental group after treatment were all significantly lower than those of control group at the same period, the differences were all statistically significant (P ＜ 0.05). Conclusion Yougui Pills combined with ginger moxibustion in the treatment of knee osteoarthritis with syndrome of yang deficiency and coagulated cold has remarkable curative effects, which can significantly improve the inflammatory symptoms and immunity, relieve pain, with high safety.
2018 Vol. 15 (10): 106-109 [Abstract] ( 200 ) HTML (1 KB)  PDF (701 KB)  ( 133 )
110 Correlation analysis between distribution features of tongue manifestations and some risk factors of the heart failure in dialysis patients
WANG Xiaolan1 XIAO Yonghua1 REN Chuanyun1 CAO Po1 LIU Yongguo2 YANG Shuming2 YIN Di1 ZHONG Jian1
Objective To investigate the correlation between the distribution of tongue image in patients with maintenance hemodialysis and some indices of the heart failure. Methods From August 2016 to April 2017, 77 cases of MHD patients (≥3 months) from Dongzhimen Hospital of Beijing University of Chinese Medicine and the First Integrated Traditional Chinese and Western Medicine Hospital were collected tongue information uniformly with the portable tongue image instrument. The tongue image was imported into the computer software "TCM expert system of tongue diagnosis". Three experts who are over deputy director of the physician level quantitated the 16 parameters of tongue image including the tongue spirit, the tongue color, the tongue shape, the teeth marks, cracks and etc to analyze MHD patients' distribution features of tongue manifestations; at the same time, the blood samples were took on the first day of tongue shooting, including blood routine, renal function, ion detection, serum albumin, BNP before hemodialysis, and the urea clearance index (Kt/V), the rate of urea decline (URR), the rate of interdialysis weight growth were calculated, then the correlation between tongue manifestations and objective indices was analyzed and the rules from it was found. Results Among the 77 patients undergoing maintenance hemodialysis, 41 cases were male and 36 cases were female. The score of teeth-marked tongue was positively associated with the rate of interdialysis weight growth (r = 0.488). The score of purple tongue, dark tongue, ecchymosis and thick coating were positively associated with BNP, which was positively correlated with congestion tongue (the value of r was in turn 0.532, 0.491, 0.525, 0.332, all P ＜ 0.01). Conclusion There is a positive correlation between the teeth-marked tongue and the rate of interdialysis weight growth in the maintenance hemodialysis patients. The congestion tongue is positively associated with the serum BNP. So the congestion tongue with teeth marks can be used as a reference for the hemodialysis patients with potential heart failure.
2018 Vol. 15 (10): 110-113 [Abstract] ( 183 ) HTML (1 KB)  PDF (688 KB)  ( 89 )
114 Ideas and methods of pharmacological research on TCM tonic medicine
Sarmad Sheraz Jadoon1 DING Li2 ZHANG Shunbo2 LI Fan2 YOU Qiuyun2
Tonic medicine is one of the important chapters in courses such as "Chinese materia medica", "pharmacology of traditional chinese Medicine" and some others. In order to make students better grasp the contents of this chapter, and apply it both to real life and to TCM clinical treatment, this article explores the ideas and methods of researches on tonic medicines from the four aspects including the functions of tonic medicines in regulating the nerve-endocrine-immune system, the effects of tonic medicines on metabolism, the exploration on animal models with deficiency syndromes and varied research focuses of different tonic medicines. The purposes of the research are to establish the scientific methods and approaches for researches on tonic medicines, and lay a solid foundation for better explaining the scientific connotation of the medical treatment by tonic medicines under the guidance of TCM theories and for providing guidance to the scientific and rational doses of TCM clinical treatment.
2018 Vol. 15 (10): 114-117 [Abstract] ( 227 ) HTML (1 KB)  PDF (654 KB)  ( 177 )
118 The value of CO2-DSA in the diagnosis of small intestinal bleeding
LU Wei YANG Chao ZHANG Jiacheng YANG Jian DU Peng MA Junpeng ZHANG Zhe JIANG Fuqiang
Objective To compare the role of CO2-DSA and non-ionic iodine contrast agent in diagnosis of intestinal bleeding. Methods 20 cases of patients with small intestinal bleeding treated in Navy General Hospital of People′s Liberation Army from October 2015 to June 2017 were selected. Superior and inferior mesenteric arteries angiography were performed in 20 patients by use of both CO2 and iodinated contrast agent. Repeated angiography was performed with super-selective catheterization of the third level branches of suspected arteries. The images were evaluated blindly. The positive depicted of bleeding in both CO2 and iodine contrast agents were compared. Results Small intestinal bleeding was demonstrated in 7 cases (7/20) with CO2-DSA, while 9 cases with iodine contrast agent-DSA (9/20), there was no significant difference between the two groups (χ2=0.417, P > 0.05). Small intestinal bleeding was showed in 19 of the 20 cases by CO2-DSA (95.0%), while 14 cases (70.0%) by iodine contrast agents, with statistically significant difference (χ2=4.329, P < 0.05). Conclusion CO2 gas is superior to iodine contrast agent in demonstrating small intestinal bleeding in case of super-selective catheterization near the bleeding arteries.
2018 Vol. 15 (10): 118-121 [Abstract] ( 190 ) HTML (1 KB)  PDF (445 KB)  ( 106 )
122 Effect of real-time three-plane automated function imaging in assessing a single hemodialysis on left ventricular longitudinal systolic function of patients with uremia
ZHANG Bingyi1 GUO Ruiqiang2▲ ZHOU Qing2 PING Jie1 ZHANG Ling1 ZHANG Xiaofeng1
Objective To assess the immediate impact of preload reduction caused by single hemodialysis on left ventricular longitudinal systolic function in uremic patients with real-time three plane (real-time three-plane, RT-3PE) automatic functional imaging (AFI) technology. Methods Thirty-one patients with uremia were selected who were underwent routine hemodialysis in People′s Hospital of China Three Gorges University and Renmin Hospital of Wuhan University from December 2013 to March 2017. Left ventricular peak systolic longitudinal strain (PSLS) and bovine eye diagram were detected by using RT-3PE AFI technology before and after routine hemodialysis immediately. The change of indicators was compared before and after routine hemodialysis. Results Compared with pre-hemodialysis, post- hemodialysis the LVEF values had no statistically significant changed (P > 0.05), the average values of left ventricular whole PSLS were significantly reduced (P < 0.05). Segmental analysis showed that, the values of local PSLS only appeared in the middle period decreased significantly after hemodialysis (P < 0.01). There was no significant change in the apex section and basal segment (average P > 0.05). After hemodialysis, left ventricular overall PSLS and LVEF was significantly negative correlated (r = -0.78, P < 0.01), and Sm also showed significant negative correlation (r = -0.50, P < 0.50). While left ventricular overall PSLS and dialysate volume was significantly positive correlated (r = 0.63, P < 0.05). Conclusion As showing by the results of PSLS measured by AFI analysis, application of left ventricular PSLS can accurately evaluate left ventricular longitudinal contraction function in patients with uremia. Under the condition of no changing the whole systolic function of left ventricular, single dialysis can affect the left ventricular longitudinal systolic function by reducing preload, significant effects change only appeared in the middle section. When using PSLS assessment of left ventricular systolic function in uremia patients, doctors should pay attention to the state of preload.
2018 Vol. 15 (10): 122-126 [Abstract] ( 239 ) HTML (1 KB)  PDF (645 KB)  ( 80 )
127 Pharmaceutical care for a patient with parapsilosis Candidaemia treated with high dose Fluconazole
ZHONG Ling1 LOU Yan2 LI Ben1
Fluconazole, a three azole antifungal agent, is widely used in the treatment of Candida and Cryptococcal infections. However, with the increasingly prominent problem of Candida resistance, the choice of antifungal drugs and the use of dose has become a difficult point in clinical medication. This paper makes analysis for a case with parapsilosis Candidaemia treated with high dose Fluconazole, from the angle of disease diagnosis, treatment and the safety of high dose Fluconazole, combined with pharmaceutical car, and evaluates and intervenes the safety and rationality of drug use. The article provides reference for clinical rationly application of Fluconazole in the treatment of parapsilosis Candidaemia.
2018 Vol. 15 (10): 127-130 [Abstract] ( 214 ) HTML (1 KB)  PDF (399 KB)  ( 102 )
131 Evaluation and application of uncertainty in HbA1c measurement by ion exchange chromatography
DIAO Qizhi1 HUANG Lijun1 MO Zhan1 ZENG Qiang1 WANG Kaihua2▲
Objective To evaluate the uncertainty of glycosylated hemoglobin (HbA1c) measuredby ion-exchange chromatography, and todiscussthe application value of uncertainty in clinical diagnosis and treatment. Methods The source of uncertainty were combed from pre-analysis, analytical phase, physiological variation, some diseases influence factors, and so on. Then the standard uncertainty and expanded uncertainty were calculated and the clinical applications of uncertainty in clinical diagnosis and treatmentwere illustrated. Results The main source of the uncertainty on HbA1c measured by ion exchange chromatography were imprecision, systematic error, the uncertainty of calibration product and errors caused by calibration product redissolved during measurement. The pre-analysis factors could be controlled by pre-analysis quality control. Physiological variation and other disease affecting factors wereonly related to some individuals without universality, which could not taken into consideration. The relative standard uncertainties of the internal quality control concentrationsin 5.11% and 10.04% were 6.33% and 4.54% respectively, the standard uncertainties were 0.32% and 0.46% respectively and the expanded uncertainty were 0.64% and 0.92% respectively. Two HbA1c measurements near by 5.11%, 10.04% for respectively. Taking the uncertainty into consideration, the possibility of suffering from diabetes could be excluded when HbA1c concentration of patient ＜5.86%, in addition diabetes could be diagnosed when HbA1c>7.14%. While patientscould not be determined whether suffering from diabetes when HbA1c value is located between 5.86% and 7.14%, which needed further examination to confirm. Conclusion The main source of the uncertainty on HbA1c measurement by ion exchange chromatography are measurement imprecision, systematic error, the uncertainty of calibrator productand the errors caused by calibrator product redissolving. The uncertainty is significant to diagnose clinical diseases and evaluate curative effect.
2018 Vol. 15 (10): 131-134 [Abstract] ( 209 ) HTML (1 KB)  PDF (480 KB)  ( 127 )
135 Application effect of anti-D immunoglobulin on Rh negative blood pregnant women
Zhang Jing LU Xin SUN Xuan PAN Li CHEN Lidong
Objective To dicuss  the application effect of anti-D immunoglobulin on Rh negative blood pregnant women. Methods From January 2016 to November 2017, in Haidian District Maternal and Child Health Care Hospital, 119 pregnant women with Rh negative blood were selected, 33 pregnant women injected with anti-D immunoglobulin by themselves at 28 weeks of pregnancy were as the injected anti-D immunoglobulin group (experiment group), and 33 randomly selected from 86 pregnant women not received anti-D immunoglobulin were as the control group. The anti-human globulin detection card of Diana microcolumn gel was applied to test the anti-D antibody of the Rh negative blood pregnant women, and the RhD blood type was tested by test tube method, and the difference of two groups were statistically analyzed. Results The postpartum hemorrhage, neonatal infection, premature birth, stillbirth and neonatal anemia of experiment group and control group were compared, the differences were not statistically significant (P > 0.05). The mode of delivery, proportion of Rh-negative newborns, neonatal hyperbilirubinemia of two groups were compared, the differences were statistically significant (P < 0.05) Conclusion Injecting anti-D immunoglobulin is an effective way to avoid alloimmunization to produce anti-D antibody, it can reduce incidence of Rh immunosuppression, the management of anti-D immunoglobulin in our country needs to be improved.
2018 Vol. 15 (10): 135-138 [Abstract] ( 249 ) HTML (1 KB)  PDF (461 KB)  ( 205 )
139 Discussion on the application of learning and memory science in pharmacology teaching
LI Xiufang LIN Qing YU Zepu ZHOU Ningna
The learning and memory includes three processes, which are the gain, storage, recall of information. The important guarantee of improving the effects of learning and memory is that get out the stored information successfully by avoiding interference phenomenon, which depends on whether the information has been encoded with a favorable clue or not. From the view of neuroscience mechanism of learning and memory, taking the difficult teaching point drug transmembrane transport in pharmacology course as example, this paper discusses the method of applying the neuroscience mechanism of learning and memory to improve teaching quality of pharmacology. In the teaching of pharmacology, application of neuroscience mechanism of learning and memory can connect new knowledge (new information) with original information and within new information, integrate fragmented information to form a desired memory cues, avoid interference effectively, get the best learning and memory effect, and help students to improve learning efficiency, which is worthy of promotion and application in the teaching of pharmacology and other courses.
2018 Vol. 15 (10): 139-141 [Abstract] ( 163 ) HTML (1 KB)  PDF (477 KB)  ( 90 )
142 The practical effect of integrating ISO 15189：2012 elements into teaching practice of clinical immunology
CHENG Yong LIU Chengeng CAO Jingrong WANG Peichang
Objective To evaluate the value of ISO 15189:2012 elements into the teaching of clinical immunology practice. Methods A total of 62 clinical laboratory students in Xuanwu Hospital, Captital Medcial University, Capital Medical University Yanjing Medical School, and Beijing Health School from 2015 to 2017 were divided into experimental group and control group, and each group was 31 students. The control group adopted the traditional teaching method, and the experimental group integrated the ISO 15189:2012 elements into the practice band teaching. The theoretical knowledge, operating skills, comprehensive performance, teaching effects evaluation, and employer evaluation of two groups were compared. Results The theoretical knowledge, practical operation ability, Interpretation report, and comprehensive performance in after-department examination of the experimental group were higher than those of the control group, the differences were statistically significant (P ＜ 0.05). The recognition rate of teaching methods, communication skills and theoretical level in the experimental group were higher than those of the control group, the differences were statistically significant (P ＜ 0.05). The teaching attitude to teachers of the two groups had no statistically significant difference (P > 0.05). Peer teachers grade of the experimental group was better than that of the control group, professional skill, standardized work, and quality document execution of the experimental group were higher than those of the control group, the difference was statistically significant (P ＜ 0.05). Conclusion The integration of the elements of ISO 15189:2012 into the clinical immunological practice is conducive to improving the professional knowledge, increasing the satisfaction of employers, and providing a good reference value for the clinical laboratory diagnosis teaching.
2018 Vol. 15 (10): 142-145 [Abstract] ( 208 ) HTML (1 KB)  PDF (528 KB)  ( 71 )
146 Thought about the humanistic quality education of medical students keeping pace with the times in view of ideological and political education
XIA Xingkun1 CHENG Huaizhi2 KONG Lingshan3
The quality of medical students' humanistic quality education is directly related to the comprehensiveness of medical personnel training, the smooth transition of medical model and the improvement of doctor-patient relationship. At present, there are many problems in higher medical education in China, such as the lack of humanistic quality education concept and the weak of education content. How to explore a new way to cultivate medical humanistic spirit is an important issue in the field of higher medical education in view of ideological and political education. Guided by the marxism, based on the reference to the education, philosophy, medicine and other related disciplines, centered on the content and necessity of medical students' humanistic quality education in contemporary China, this paper summarizes the current situation and existing problems. It is believed that the concept of education should be updated first, and the development concept of equal scientific and humanistic quality should be set up. Secondly, we should refer to the successful cases of developed countries to build the education curriculum system and education and assessment mechanism of humanistic quality.
2018 Vol. 15 (10): 146-149 [Abstract] ( 179 ) HTML (1 KB)  PDF (524 KB)  ( 111 )
150 The comparative study of glidescope combined with glowstick in trachea intubation of clinical anesthesia
ZHAO Bo1 WU Yang1 GAO Wenwei2 HOU Jiabao1 ZHAN Liying1
Objective To explore the comparative study of glidescope combined with glowstick in trachea intubation in intern students of clinical anesthesia. Methods Twenty-four intern students from September 2016 to April 2017 in Anesthesiology Department of Renmin Hospital of Wuhan University were randomly allocated into 4 groups by random numbers table method: normal intubation group (group G), glidescope intubation group (group A), glowstick intubation group (group B) and glidescope combined with glowstick intubation group (group C), with 6 each group. Before trachea intubation, according to the air way assessed by Mallampati and Comark-Lehane patients were divided into normal air way and difficult air way, 12 in each group. The successful rate and time of trachea intubation at the last week of 1,3 months and 6 months were recorded. Results After 1 month, the successful rate of intubation was no difference between four groups, however, the time of intubation was little longer in group B on normal air way (P < 0.05), the successful rate of intubation in group C were higher than other groups, all groups need more time for intubation on difficult air way (P < 0.05). After 3 months, there were no difference between four groups on normal air way (P > 0.05), the successful rate of intubation in four groups were increased than 1 month ago, but group C was still higher than other groups on difficult air way (P < 0.05). After 6 months, the successful rate of intubation in A, B and C groups was higher than that of the group G, group C was the highest group on difficult air way (P < 0.05). Conclusion Glidescope combined with glowstick can quickly improve the successful rate of trachea intubation for the abecedarian, and it has a great advantage in the intubation of patients with difficult air way.
2018 Vol. 15 (10): 150-153 [Abstract] ( 265 ) HTML (1 KB)  PDF (495 KB)  ( 140 )
154  Experience of constructing the harmonious doctor-patient relationship in our hospital
ZHANG Taojing1 HU Kaiwen2 ZHAO Chang1▲ XU Weina1 ZHANG Huishu1
In recent years, the doctor-patient conflicts, the medical dispute rate increased significantly, the difficulty of medical dispute mediation is more and more serious, and even disrupt the normal working order of hospital and medical personnel safety against malignant medical disputes, which caused a bad influence. With the new stage of health care reform, medical institutions attach importance to constructing a harmonious doctor-patient relationship increasingly. In order to solve patients problems effectively, Dongfang Hospital of Beijing University of Chinese Medicine (“our hospital” for short) has tried to make the solution more unimpeded through the cultural construction, institutional improvement, preventive training, implement incentive and disincentive, active communication and quick fixe since 2014. As a result, the amount of medical compensation has decreased by 89% and complaints have decreased by 11%. Meanwhile, the patient satisfaction has been greatly improved. A harmonious doctor-patient relationship has been constructed. This paper shares some experiences of building harmonious doctor-patient relationship in our hospital in the past few years, and builds a harmonious doctor-patient relationship with the power of the whole hospital.
2018 Vol. 15 (10): 154-157 [Abstract] ( 203 ) HTML (1 KB)  PDF (419 KB)  ( 115 )
158 Effect of comprehensive nursing care on Duhamel modified surgery for Hirschsprung's disease
XIA Hongxia WANG Fang LIAO Xiaofeng WANG Yuzhen
Objective To investigate the effect of comprehensive nursing care on Duhamel modified surgery for Hirschsprung's disease. Methods From January 2016  to June 2017, in Xiangyang Centrol Hospital, 80 Hirschsprung disease children were selected, according to random number table method, they were divided into observation group and control group, with 40 cases in each group, the two groups underwent modified Duhamel surgery, the control group received routine nursing, the observation group received nursing intervention. The curative effect, complication rate and nursing satisfaction of the two groups were compared. Results The total effective rate in the observation group was 95%, that in the control group was 90%, the difference was not statistically significant (P > 0.05); the complication rate in the observation group (5%) was that lower than that in the control group (30%), the difference was statistically significant (P < 0.01). The total satisfaction rate in the observation group (97.50%) was lower than that in the control group (62.50%), the difference was statistically significant (P < 0.01). After nursing, the quality of life scores in the observation group [(58.66±5.28) scores] was higher than that in the control group [(47.41±5.37) scores], the difference was statistically significant (P < 0.05). Conclusion The comprehensive nursing effect of modified Duhamel operation is effective, it can significantly reduce the incidence of complications, improve the satisfaction of patients and their families and it is worthy of popularization and application.
2018 Vol. 15 (10): 158-160,173 [Abstract] ( 178 ) HTML (1 KB)  PDF (594 KB)  ( 114 )
161 Preliminary compilation and reliability and validity test of family pension burden scale
WANG Jie WANG Ying ZHANG Yuanyuan WANG Aihua DING Xiao CUI Min
Objective To compile the family pension burden scale and test its reliability and validity, in order to provide a tool for measuring the burden of family support. Methods Through the literature review and interview method, the family pension burden initial scale was compiled and 210 women of childbearing age in Shandong Province sampled by cluster sampling was used to assess the validity and reliability. Results The burden of family pension scale consisted of 4 dimensions and 21 items: Burden of activity (7 entries), physical and mental burden (6 entries), economic burden (5 entries), and emotional burden (3 entries). The Cronbach's α of the total table was 0.933, the half half reliability coefficient was 0.885, the content validity index of the total table was 0.905, and the cumulative validity of the 4 factors was 65.658%. Conclusion The preliminary family pension burden scale has good reliability and validity, which can be used as a tool to assess the burden of family support.     
2018 Vol. 15 (10): 161-164 [Abstract] ( 183 ) HTML (1 KB)  PDF (634 KB)  ( 140 )
165 Application effect of WeChat group in continuous nursing care of postoperative patients with indwelling double J tube in Department of Urology
ZHANG Lijuan BI Qingquan▲
Objective To explore the application effect of WeChat group in extended nursing in patients with Double J stents placement afer surgery. Methods From June 2016 to May 2017, in Department of Urology, Fourth Affiliated Hospital of Anhui Medical University, 90 patients with indwelling double J tube and discharged were selected, according to the random number table method, they were divided into observation group and control group, with 45 in each group. The control group received follow-up by regular health education and telephone, the observation group received extended care using WeChat group additionally. After the end of the catheter, the incidences of complications and self-care ability and satisfaction of patient were compared between two groups. Results The incidences of various complications and repetitive rate of nursing labor power in the observation group were significantly lower than those in the control group, the differences were statistically significant (P < 0.05). In the observation group, the knowledge of the indwelling double J tube, the prevention of complications, the mastery of the countermeasures, the satisfaction degree and the total score of the continuing nursing education were higher than those in the control group, the differences were statistically significant (P < 0.05). Conclusion The application of WeChat group for extended care in discharged patients with double J stents placement after surgery in Department of Urology can significantly reduced the incidence of complications and improve self-care ability and quality.
2018 Vol. 15 (10): 165-168 [Abstract] ( 216 ) HTML (1 KB)  PDF (607 KB)  ( 92 )
169 Meta-analysis of transitional care intervention on quality of life for adult patients with asthma
LAI Jiahao1 GAO Zhen2 ZHANG Gaohua3 GAN Yuhong4 LI Wen5▲
Objective To assess the effect of transitional care intervention on quality of life for adult patients with asthma. Methods PubMed, Cochrane, Embase, WanFang data, CNKI (China National Knowledge Infrastructure), Chinese scientific journals full-text database, CBMdisc (China Biology Medicine disc) were searched for continuity of patient care with conventional treatment randomised controlled trials (RCT) (published from established the database to January 31 2017; no language restrictions). They were divided into two groups named ACT group and AQLQ group according to research index that ACT and AQLQ. The data were analyzed by using Revman 5.2 software. Results ACT group involved 12 RCT, 684 cases in experiment group and 598 cases in control group. The scores of experiment group were higher than control group in ACT group, with combined effect of the amount of random effects mode: WMD = 3.13, 95%CI: 2.26-4.00, P < 0.01. AQLQ group involved 13 RCT, 641 cases in experiment group and 628 cases in control group. The scores of experiment group were higher than control group in AQLQ group, with combined effect of the amount of random effects mode: WMD = 3.72, 95%CI: 3.98-6.46, P < 0.01. Conclusion Transitional care can improve the quality of life of adult patients with asthma.
2018 Vol. 15 (10): 169-173 [Abstract] ( 169 ) HTML (1 KB)  PDF (1349 KB)  ( 132 )
174 Effect of Orem self-care theory on psychological status and prognosis of patients with gastric ulcer
LIANG Yanfang
Objective To analyze the influence of psychological status and  self-care ability after gastric ulcer patients treated with Orem self-care theory. Methods From October 2015 to October 2017, in Xiaolan People′s Hospital Affiliated to Southern Medical University, 120 gastric ulcer patients were selected, they were divided into observation group and control group by random envelope method, with 60 cases in each group. The control group was given routine nursing intervention program, the observation group was given Orem intervention programme self-care theory. The nursing satisfaction of patients was assessed before and after nursing, patients′ self-care ability was assessed by andusing self-nursing can scale; anxiety and depression of patients were assessed by the self-rating anxiety scale (SAS) and self-rating depression scale (SDS). Results After the treatment, the health knowledge, self-concept, self-care ability and self-care responsibility score increased significantly in two groups, and the indexs in observation group were significantly higher than the control group, the differences were statistically significant (P < 0.05). The SAS and SDS scores in two groups reduced after nursing, and the observation group was significantly lower than the control group, the differences were statistically significant (P < 0.05). The satisfaction degree of the observation group was significantly higher than the control group, the differences were statistically significant (P < 0.05). Conclusion Under the intervention of Orem self-care theory, the patients with gastric ulcer can significantly improve the psychological status and improve the quality of prognosis, it has a wide range of clinical application value.
2018 Vol. 15 (10): 174-176,181 [Abstract] ( 227 ) HTML (1 KB)  PDF (701 KB)  ( 65 )
177 Effect of motivational interview combined with IKAP model on compliance behavior and quality of life in elderly patients with advanced cancerous pain of gastric cancer
HU Cen1 LI Shuling1 YUAN Liu2▲
Objective To explore the effect of the motivational interview combined with the information knowledge attitude and practice (IKAP) model on the compliance behavior and the quality of life in elderly patients with advanced cancerous pain of gastric cancer. Methods From May 2015 to May 2017, in 161st Hospital of PLA, 69 elderly patients with advanced cancerous pain of gastric cancer were selected, according to random number table, they were divided into two groups, the control group (34 cases) was given routine nursing intervention, observation group (35 cases) was given motivational interview combined with IKAP model on the basis of routine nursing. After 3 months of intervention, the remission, control status of cancer pain and compliance behavior were observed and compared in the groups. The nursing satisfaction and quality of life (QOL) scores of two groups were recorded. Results  The total remission rate of the observation group was 91.43% (32/35), it was higher than that of the control group (67.65%, 23/34), the difference was statistically significant (P < 0.05). After the intervention, the degree of worrying about the addiction of anesthesia analgesia, out-of-control pain, drug resistance and side effects and the doctors' treatment score of the observation group were lower than those of the control group, and the QOL score was higher than that of the control group, the differences were statistically significant (P < 0.05). The total compliance rate of the observation group was 88.57% (31/35), it was higher than that of the control group (67.65%) (23/34), the difference was statistically significant (P < 0.05). The nursing satisfaction rate of the observation group was 85.71% (30/35), it was higher than that of the control group (64.71%, 22/34)， the difference was statistically significant (P < 0.05). Conclusion IKAP model combined with motivational interview can improve the compliance behavior of elderly patients with advanced cancerous pain of gastric cancer, reduce the degree of pain, and it contributes to improve the quality of life, and it has high degree of nursing satisfaction.
2018 Vol. 15 (10): 177-181 [Abstract] ( 744 ) HTML (1 KB)  PDF (739 KB)  ( 125 )
Copyright © Editorial Board of 中国医药导报
Supported by:Beijing Magtech