山姜素抑制卵巢癌OVCAR-8细胞增殖迁移及其机制研究

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Abstract Objective To explore the effect of alpinetin on human ovarian cancer cell line OVCAR-8 and its mechanism. Methods OVCAR-8 cells were treated with different concentrations of alpinetin (25, 50, 100, 200 and 400 μmol/L, DMSO as control) for different hours (24, 48 and 72 h), then cell viabilities were detected by CCK-8 assays. 3D spheroid assay was used to measure the spheroid formation capacity of OVCAR-8 cell after treated with 50 μmol/L alpinetin (DMSO as control) for 7 days. Besides, wound healing assay was used to measure the migration capacity of OVCAR-8 cell after treated with 100 μmol/L alpinetin (DMSO as control) for 24 h. In addition, Western blot was used to detect the protein levels of STAT3 signaling related and apoptosis related proteins in OVCAR-8 cells after alpinetin treatments (50, 100 and 200 μmol/L, DMSO as control) for 48 h. Results 25-400 μmol/L alpinetin had remarkable inhibiting effects on OVCAR-8 cells and in a dose-and time-dependent manner(all P ＜ 0.05). After 7 days′ treatment, the diameter of spheroid in 50 μmol/L alpinetin group was significantly shorter than DMSO control group (P ＜ 0.05). Besides, the width of wound in 100 μmol/L alpinetin group was significantly longer than DMSO control group after treatment for 24 h (P ＜ 0.05). In addition, the protein levels of Bcl-2, p-STAT3, c-myc and survivin were decreased in 50, 100 and 200 μmol/L group of OVCAR-8 cells than DMSO control group after 48 exposure. On the contrary, the protein level of Bax, cleaved-caspase-3 and -9 was increased (all P ＜ 0.05), and there were no significantly changes in STAT3 levels (P ＞ 0.05). Conclusion Alpinetin can inhibit the proliferation and migration of OVCAR-8 cells,its mechanism may be related to the inhibition of STAT3 signaling pathway and the activation of mitochondrial apoptosis signaling．

Key words： Alpinetin Ovarian cancer Proliferation Migration Spheroid Apoptosis

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	ZENG Jing WANG Jing▲ YIN Jinfeng SONG Qi

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ZENG Jing WANG Jing▲ YIN Jinfeng SONG Qi. Study of the inhibition effect of alpinetin on human ovarian cancer cell line OVCAR-8 and its mechanism[J]. 中国医药导报, 2018, 15(10): 13-17.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I10/13

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