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| Study on the quality standard and preliminary pharmacodynamics of Freeze-dried Toad Skin Gel |
| LU Hongyan1 HUANG Shengwu2▲ |
1.Xisha Department of Pharmacy, Sir Run Run Shaw Hospital Affiliated to Zhejiang University School of Medicine, Zhejiang Province, Hangzhou 310000, China;
2.School of Pharmacy, Zhejiang University of Traditional Chinese Medicine, Zhejiang Province, Hangzhou 310053, China |
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Abstract Objective To establish the quality standard and preliminary pharmacodynamic study of Freeze-dried Toad Skin Gel. Methods TLC was used for the qualitative identification of Cinobufagin and Resibufogenin, HPLC was adopted to determine content of Cinobufagin and Resibufogenin. Chromatographic column: kromasil C18 (4.6 mm×250 mm, 5 μm); flowing phase: acetonitrile (A)- water (B) (48∶52); flow rate: 1 mL/min; UV detection wavelength: 296 nm; column temperature: 30℃; the volume: 10 μL. S180 ascites solid tumor mice were used to study the pharmacodynamics of inhibition of tumor growth. Results The fluorescence spots of the same color were shown in the corresponding position of the Silica gel G thin layer, and the spot size was moderate and the separation degree was good. Cinobufagin and Resibufogenin showed good linearity between the peak area and the concentration in the range of 0.056-4.48 μg/mL (r=0.9987) and 0.256-10.24 μg/mL (0.9996), respectively. The sample recovery rate(n=6) was 100.27%, 101.68%, RSD was 1.56% and 1.67%. The Preliminary pharmacodynamics study showed that all groups of Freeze-dried Toad Skin Gel had significant anti-tumor effect, and the high-dose group had the best effect, with the inhibitory rate of 48.03%. Conclusion The quality standard of Freeze-dried Toad Skin Gel is established, and the preliminary pharmacodynamics shows that it has a significant anti-tumor effect.
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[1] 张振海，王晋艳，陈彦，等.不同品种及产地蟾皮中抗肿瘤活性成分含量比较[J].中华中医药杂志，2011，26（11）：2698-2701.
[2] 樊佳佳，宋明，宋驰，等.中药材蟾皮及其混伪品的DNA 条形码鉴定研究[J].中国药学杂志，2015，55（15）：1292-1296.
[3] 张振海，王晋艳，陈彦，等.不同品种及产地蟾皮中抗肿瘤活性成分含量比较[J].中华中医药杂志，2011，26（11）：2698-2701.
[4] 王燕华，金春爱，孙印石，等.不同加工方式的鹿茸脂肪酸的气相色谱分析[J].中草药，2017，48（12）：2431-2440.
[5] 辛少鲲，司南，王宏洁，等. 蟾皮中亲水性成分的化学研究[J].中国中药杂志，2016，41（20）：3767-3772.
[6] 赖宝林，王利胜，张升，等.中药凝胶剂的研究进展[J].中药新药与临床药理，2010，21（2）：211-213.
[7] 谷福根，张艺馨，王毅，等.甲磺酸雷沙吉兰鼻用凝胶剂的制备及质量评价[J].中国新药杂志，2017，26（3）：351-355.
[8] 邱仁杰，黄艺钟，王秀丽.乳剂型凝胶剂预混辅料的初步研制[J].中国中药杂志，2017，42（3）：498-504.
[9] 曾明辉，熊静悦，朱九群，等.析因设计优化塞来昔布凝胶中透皮促渗剂用量研究[J].中国药学杂志，2017，52（4）：293-296.
[10] Munarin F，Tanzi MC，Petrini P. Advances in biomedical applications of pectin gels [J]. Int J Biol Macromol，2012， 51（4）：681-689.
[11] Peeters J，Neeskens P，Tollenaere JP，et al. Charaeterization of the interation of 2-hydroxypropyl-β-cyclodextrin with itraconazole at pH 2，4 and 7 [J]. J Pharm Sci，2002， 91（6）：1414-1422.
[12] Wang DL，Qi FH，Tang W，et al. Chemical constituents and bioactivities of the skin of Bufo bufo gargarizans Cantor [J]. Chem Biodivers，2011，8（4）：559-567.
[13] 郝敏，陆兔林，毛春琴，等.基于中药质量标志物的饮片质量控制研究[J].中草药，2017，48（9）：1699-1707.
[14] 张永文.试谈中药新药质量标准制定的整体思路[J].中国新药杂志，2017，26（18）：2185-2189.
[15] 黄玉叶，宋霄宏.蟾皮的提取和含量测定及临床应用研究进展[J].中华中医药学刊，2011，29（7）：1636-1638.
[16] 昝日增，胡万杨，黄玉叶，等.干蟾皮中蟾毒内酯类成分的提取工艺研究[J].中草药，2011，42（7）：1330-1332.
[17] 谢红梅，赵丹丹，李雅雅，等.蟾皮凝胶剂制备工艺优选及体外透皮性能考察[J].中国实验方剂学杂志，2014， 20（12）：21-24.
[18] 张璐璐，张维东，武力存，等.蝎毒多肽提取物促进自噬抑制S180肉瘤作用机制的研究[J].中草药，2015，46（8）：1190-1194.
[19] Liu R，Li J，Zhang T，et al. Itraconazole suppresses the growth of glioblastoma through induction of autophagy：Involvement of abnormal cholesterol trafficking[J]. Autophagy，2014，10（7）：1241-1255.
[20] 郑婷婷，付艳丽，焦丽静，等.解毒散结方醇提物抗裸小鼠人肺癌术后转移作用的研究[J].中药新药与临床药理，2016，27（6）：815-821.
[21] 张璐璐，张维东，武力存，等.蝎毒多肽提取物促进自噬抑制S180肉瘤作用机制的研究[J].中草药，2015，46（8）：1190-1194. |
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