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| Application of pepsinogen, thymidine kinase 1 joint detection in the screening for gastric cancer |
| LI Weibing1 WANG Kuiming1 HOU Weiwei1 YU Haizhong1 YUAN Jianfen1 YAO Hui2 XIAO Chunhong3▲ |
1.Department of Laboratory Medicine, Nantong Hospital of Traditional Chinese Medicine, Jiangsu Province, Nantong 226001, China;
2.Department of Laboratory Medicine, Nantong Maternal and Children Health Care Service Hospital, Jiangsu Province, Nantong 226000, China;
3.Department of Laboratory Medicine, Nantong Tumor Hospital, Jiangsu Province, Nantong 226361, China |
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Abstract Objective To investigate the value of pepsinogen (PG) and thymidine kinase 1 (TK1) combined detection for gastric cancer screening. Methods Two hundred and fifty-eight cases were included in the research from February 2016 to June 2017, who were stomach cancer (n=178) [including early gastric cancer (n=46), progress gastric cancer (n=60)], atrophic gastritis (n=72), and healthy individuals (n=80) as control respectively. Blood PG and TK1 were assayed with enzyme linked immonosorbent assay (ELISA) and Western blot-enayme immunoassay chemilumiescence commercial kit. Results PGⅠand PGⅠ/Ⅱ(PGR) of gastric cancer were lower than those of atrophic gastritis and normal control (P < 0.05), and which of advanced gastric cancer were lower than those of early gastric cancer (P < 0.05). TK1 of gastric cancer was higher than that of atrophic gastritis and normal control patients (P < 0.05), which had nothing to do with the staging of gastric cancer (P > 0.05). Sensitivity and specificity for PG and TK1 were 52.1%, 81.9% and 47.2%, 86.1% respectively. Sensitivity and specificity for PG and TK1 combined assay were 81.1% and 79.2%, which were significantly higher than those of individual assay (P < 0.05). Conclusion This study shows that PG and TK1 combined detection has excellent value for gastric cancer screening.
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[1] DeVries AC，Haringsma J，Kuipers EJ. The detection surveillance and treatment of premalignant gastric lesions related to Helicobacter pylori in fection [J]. Helicobacter，2007， 12（1）：1-15.
[2] Chen W，Zheng R，Baade PD.Cancer statistics in china 2015 [J]. CA Cancer J Clin，2016，66（2）：115-132.
[3] 顾同进，黄海.早期胃癌的诊断与治疗进展[J].老年医学与保健，2015，21（4）：210-211，214.
[4] Koga M，Miyakawa K，Ikeda S. Annual Report of Gastroenterological Mass Survey in Japan，2002 [M]. Tokyo：Japanese Society of Gastroenterology MassSurvey，2004.
[5] 吴钢，赵昌杰，姜琦，等.胃癌血清标志物的临床意义及研究进展[J].中国当代医药，2016，23（14）：10-12.
[6] 房静远，刘文忠，李兆申，等.中国慢性胃炎共识意见（2012年，上海）[J].中国医学前沿杂志：电子版，2013，5（7）：44-55.
[7] 中华医学会消化内镜学分会，中国抗癌协会肿瘤内镜专业委员会.中国早期胃癌筛查及内镜诊治共识意见（2014年，长沙）[J].中华消化杂志，2014（7）：433-448.
[8] 梁艳丽，李凤，王欣，等.血清胃蛋白酶原检测在胃部疾病诊断中的临床意义[J].临床医药实践，2016，25（8）：594-596.
[9] 唐军梅，唐军辉.幽门螺旋杆菌感染与胃癌患者病理特征性改变的相关性研究[J].世界最新医学信息文摘，2016，16（90）：151，153.
[10] Yoshihara M，Sumii K，Haruma K，et al. Correlation of ratio of serum pepsinogenⅠand Ⅱ with prevalence of gastric cancer and adenoma in Japanese subjects [J]. Am J Gastroenterol，1998，93：1090-1096.
[11] Ohata H，Oka M，Yanaoka K，et al. Gastric cancer screening of a high risk population in Japan using pepsinogen and barium digital radio graphy [J]. CancerSci，2005，96：713-720.
[12] Dinis Ribeiro M，Yamaki G，Miki K，et al. Meta analysis on the validity of pepsinogen test for gastric carcinoma dysplasia or chronic a trophic gastritis screening [J]. Journal of Medical Screening，2004，11：141-147.
[13] Tanaka T，Nakamura J，Kitajima Y，et al. Loss of trefoil factor 1 is regulated by DNA methylation and is an independent predictive factor for poor survival in advancedgastric cancer [J]. Int J Oncol，2013，42（3）：894-902.
[14] Baker DJ，Dawlaty MM，Galardy P，et al. Mitotic regulation of the anaphase promoting complex [J]. Cell Mol Life Sci，2007，64 （5）：589-600.
[15] 朱艳哲，马泰，张从军，等.血清胸苷激酶1在肿瘤患者中的表达及临床意义[J].安徽医科大学学报，2015，50（7）：1012-1015.
[16] 程晓虎，项和平.胃癌患者检测细胞质胸苷激酶的临床意义[J].安徽医药，2015，19（8）：1571-1573.
[17] 王彬，李国锋，王健，等.血清TK1水平检测在非小细胞肺癌诊断中的应用[J]. 国际检验医学杂志，2016，37（18）：2563- 2565.
[18] 李祚品，张平安.血清AFP、TK1、DKK1联合检测对原发性肝癌的诊断价值[J].现代中西医结合杂志，2016， 25（1）：94-95，98.
[19] 刘新风，徐洪玉，尹胜菊，等.乳腺癌患者血清TK1、CA153、CEA的变化及临床意义[J].国际检验医学杂志，2016，37（17）：2392-2393.
[20] 王金亮，孟祥朝，张自立，等.结肠癌患者血清中microRNA-183及胸苷激酶1的表达及意义[J].天津医药，2017，45（1）：72-75. |
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