黄诺玛苷对高糖诱导HUVEC细胞氧化应激及VEGF和ICAM-1蛋白表达的影响

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Abstract Objective To observe the effects of flavanomarein glycosides of Coreopsis tinctoria Nutt. on oxidative stress and expression of vascular endothelial growth factor (VEGF) and intercellular cell adhesion molecule-1 (ICAM-1) protein in human umbilical vein endothelial cells (HUVEC) induced by high glucose. Methods The cultured HUVEC cells were divided into normal control group (NG group), high glucose model group (HG group) and different concentrations of flavanomarein group (50, 100, 200, 400 μmol/L). The activity of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in supernatant were detected by enzyme-linked immunosorbent assay (ELASA). The expression levels of VEGF and ICAM-1 protein in cells were detected by Western blot method. Results Compared with NG group, the content of MDA in HG group was significantly increased, the levels of SOD, CAT and GSH-Px in HG group were significantly decreased (P ＜ 0.05 or P < 0.01). Compared with HG group, after flavanomarein intervention, the content of MDA in HG group was significantly decreased, CAT, GSH-Px activity increased significantly (P ＜ 0.05). The results of Western blot showed that compared with NG group, the expression of VEGF and ICAM-1 protein in HG group increased (P ＜ 0.05 or P < 0.01). Compared with HG group, after flavanomarein intervention, the expression of VEGF and ICAM-1 protein increased with the increase of concentration followed by reduction (P ＜ 0.05 or P < 0.01). Conclusion Flavanomarein has a protective effect on HUVEC induced by high glucose may be through improving the level of oxidative stress and decreasing the levels of VEGF and ICAM-1.

Key words： Flavanomarein glycosides Human umbilical vein endothelial cells Oxidative stress Vascular endothelial growth factor Intercellular cell adhesion molecule-1

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	WANG Lili1 LI Linlin1 ZHANG Nannan1 WANG Ye1 LUO Xin1 TAO Yicun1 MAO Xinmin2

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WANG Lili1 LI Linlin1 ZHANG Nannan1 WANG Ye1 LUO Xin1 TAO Yicun1 MAO Xinmin2. Effects of flavanomarein on oxidative stress and expression of VEGF and ICAM-1 protein in HUVEC cells induced by high glucose[J]. 中国医药导报, 2018, 15(10): 8-12.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2018/V15/I10/8

[1] Wang FH，Liang YB，Zhang F，et al. Prevalence of diabetic retinopathy in rural China：the Handan Eye Study [J]. Ophthalmology，2009，116（3）：461-467.
[2] 于晓忠，李金涛，刘志广.血清胆红素水平与2型糖尿病视网膜病变的关系[J].贵阳中医学院学报，2013，35（6）：171-172.
[3] Liu M，Zhang Y，Liu X，et al. Protective effect of α-melanocyte stimulating hormone on glutamate-induced retinal excitotoxicity [J]. Zhonghua Shiyan Yanke Zazhi，2013，31（5）：440-445.
[4] 李冬明.昆仑雪菊的药学研究进展[J].浙江中医杂志，2012，47（10）：776-777.
[5] 古扎力努尔·艾尔肯，李新霞，毛新民等.新疆两色金鸡菊中绿原酸与总黄酮含量测定及指纹图谱的建立[J].西北药学杂志，2013，28（3）：248-251.
[6] 兰怡，卢伟，李琳琳，等.两色金鸡菊醇提物对实验性糖尿病大鼠血糖血脂的影响[J].中国药物应用与监测，2014， 11（5）：272-275.
[7] 木合布力·阿布力孜，张燕，景兆均，等.新疆昆仑雪菊化学成分的初步定性研究[J].新疆医科大学学报，2010，33（6）：628-630.
[8] 卢伟，兰怡，李琳琳，等.两色金鸡菊醇提物对自发性高血压大鼠血压和血浆ET，AngⅡ，CGRP的影响[J].中国实验方剂学杂志，2014，20（5）：149-153.
[9] 张瑞，李新霞，毛新民，等.HPLC法测定两色金鸡菊提取物中Flavanomarein和Marein的含量[J].安徽农业科学，2015（6）：73-74.
[10] 陈瑶，李新霞，李琳琳，等.两色金鸡菊血清药物化学研究[J].新疆医科大学学报，2017，40（4）：420-424.
[11] 李卉，张雨洁，姚蓝，等.两色金鸡菊乙酸乙酯提取物对糖尿病大鼠视网膜VEGF和ICAM1表达的影响[J].新疆医科大学学报，2017，40（4）：411-414.
[12] Tryggestad JB，Vishwanath A，Jiang S，et al. Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA [J]. Clin Sci，2016，130（21）：1955-1967.
[13] Krause BJ，Costello PM，Mu■ozurrutia E，et al. Role of DNA methyltransferase 1 on the altered eNOS expression in human umbilical endothelium from intrauterine growth restricted fetuses [J]. Clin Epigenetics，2013，8（9）：944-952.
[14] Profumo E，Buttari B，D'Arcangelo D，et al. The Nutraceutical Dehydrozingerone and Its Dimer Counteract Inflammation- and Oxidative Stress-Induced Dysfunction of In Vitro Cultured Human Endothelial Cells：A Novel Perspective for the Prevention and Therapy of Atherosclerosis [J]. Oxid Med Cell Longev，2016，2016：1246485.
[15] Adeshara KA，Diwan AG，Jagtap TR，et al. Relationship between plasma glycation with membrane modification，oxidative stress and expression of glucose trasporter-1 in type 2 diabetes patients with vascular complications [J]. Complicat，2017，31（2）：439-448.
[16] 王莉莉，刘堃，许迅，等.糖尿病性视网膜病变抗血管内皮生长因子治疗进展[J].上海交通大学学报：医学版，2012，32（2）：219-225.
[17] Morita A，Ushikubo H，Mori A，et al. A delay in vascularization induces abnormal astrocyte proliferation and migration in the mouse retina [J]. Dev Dyn，2017，246（3）：186-200.
[18] Amato R，Biagioni M，Cammalleri M，et al. VEGF as a Survival Factor in Ex Vivo Models of Early Diabetic Retinopathy [J]. Invest Ophthalmol Vis Sci，2016，57（7）：3066-3076.
[19] Fan WY，Liu NP. Meta-analysis of association between K469E polymorphism of the ICAM-1 gene and retinopathy in type 2 diabetes [J]. Int J Ophthalmol，2015，8（3）：603-607.
[20] Sim■es MJ，Lobo C，Egas C，et al. Genetic variants in ICAM1，PPARGC1A and MTHFR are potentially associated with different phenotypes of diabetic retinopathy [J]. Acta Ophthalmol，2014，232（3）：156-162.
[21] Rangasamy S，Mcguire PG，Das A. Diabetic retinopathy and inflammation：novel therapeutic targets [J]. Middle East Afr J Ophthalmol，2012，19（1）：52-59.
[22] Nakao S，Arima M，Ishikawa K，et al. Intravitreal anti-VEGF therapy blocks inflammatory cell infiltration and re-entry into the circulation in retinal angiogenesis [J]. Invest Ophth Vis Sci，2012，53（7）：4323-4328.