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2018 Vol. 15, No. 1
Published: 2018-01-05
 
 
4 Dexmedetomidine pretreatment inhibits the cerebral ischemia/reperfusion-induced neuroinflammation via AMPK/SIRT1 pathway in rats
DONG Haiping ZHOU Wei WANG Zhenhong HE Zhenzhou▲
Objective To investigate the mechanism of Dexmedetomidine (Dex) pretreatment on inflammation after cerebral ischemia/reperfusion (I/R) injury in rats. Methods Seventy-two SD rats were randomly divided into 6 groups (n=12). In sham group, after anesthesia by 3% Pentobarbital sodium (50 mg/kg), the rates were isolated the right internal carotid artery, but they were not made into the middle cerebral artery occlusion (MCAO) model. In IR group, after the molding, the rat brain was received reperfusion at 90 min after ischemia. In Dex10 and Dex50 groups, after the molding, they were intraperitoneal injected Dex (10 μg/kg or 50 μg/kg) at 30 min before ischemia. In Dex50+Yoh group, after the molding, they were intraperitoneal injected Yoh (0.5 mg/kg) before 50 μg/kg of Dex. In Yoh group, after the molding, they were intraperitoneal injected Yoh (0.5 mg/kg) before ischemia. The neurofunctional damage assessment, infarct area assessment, the levels of TNF-α and IL-1β, TUNEL staining, SIRT1 protein detection and Neuro-motor function score (TMS) in each group were detected respectively. Results In the IR group, neurofunctional scores, infarction area, TUNEL (+) cell count, levels of TNF-a and IL-1 β and SIRT1 protein expression were significantly higher than those in the sham group, while the TMS scores at 1, 2 and 5 days after reperfusion were lower than those in the sham group, with highly statistically significant difference (P < 0.01). The neurofunctional scores, infarction area, TUNEL (+) cell count, and TNF-a and IL-1 β levels in the Dex10 group and Dex50 group were significantly lower than those in the IR, Dex50+YOH and YOH group, while the TMS score and SIRT1 protein expression were higher than those in the IR, Dex50+YOH and YOH group, with highly statistically significant difference (P < 0.01). The nerve function scores, infarction area, TUNEL (+) cell count, and TNF-a and IL-1 β levels in the Dex50 group were significantly lower than those in the Dex10 group, TMS score and SIRT1 protein expression were significantly higher than those in the Dex10 group (P < 0.01). There was no statistically significant difference between the test indexes of Dex50+YOH group and YOH group (P > 0.05). Conclusion Dexmedetomidine preconditioning can activate α2 adrenergic receptor through AMPK/SIRT1 pathway to inhibit cerebral ischemia reperfusion injury in rats, and reduce the inflammation.
2018 Vol. 15 (1): 4-9 [Abstract] ( 573 ) HTML (1 KB)  PDF (809 KB)  ( 318 )
10 Effects of icariin on proliferation and apoptosis of bone mesenchymal stem cell under microgravity
ZHANG Fang1* YAN Haiyang2* CHEN Xuyi2 LYU Dan3▲ LI Li2▲
Objective To investigate the effect of icariin (ICA) on the proliferation and apoptosis of bone mesenchymal stem cell (BMSCs) under microgravity. Methods The BMSCs were randomly divided into three groups: microgravity group, dosing group and control group. The microgravity group and dosing group was treated with microgravity, besides, 1×10-6 mol/L ICA was applied to the cells in dosing group; the control group was cultured without any additional operation. Cell proliferation was detected by MTT assay, each group was set up 3 reduplicateholes; the apoptosis was detected by FCM, each group was detected 3 times; the expression of apoptosis related genes were detected by QPCR with 3 reduplicate holes, and the subcellular structure was observed by TEM. Results The result of MTT examination showed that the absorbance value of microgravity group was significantly lower than that of the control group (P < 0.01), and the dosing group was significantly higher than that of the control group (P < 0.01). FCM method showed that the apoptosis rate of microgravity group was significantly higher than control group (P < 0.01), and the apoptosis rate of the dosing group was significantly lower than control group (P < 0.01). Compared with the control group, the expression of Bcl-2, Bax/bcl-2, Bax, and P53 gene was up-regulated in microgravity group (all P < 0.05); in dosing group, the expression of Bax, Bcl-2, P53 and the Bax/bcl-2 gene was significantly down-regulated when compared with microgravity group (all P < 0.01). TEM indicated that the dosing group cells were generally normal and with no obvious changes when compared with the control group; in microgravity group, the cells existed membrane shrinking, microvilli decreasing, cytoplasmic concentration, ribosome and mitochondria gathering, nucleus pyknosis, and dense chromatin gathering to the margin of nuclear membrane. Conclusion Microgravity can inhibit osteoblast′s proliferation and promote apoptosis of BMSCs. But the appropriate concentration of ICA can protect BMSCs from the harmful effect caused by microgravity.
2018 Vol. 15 (1): 10-13,21 [Abstract] ( 329 ) HTML (1 KB)  PDF (1230 KB)  ( 157 )
14 Effect of flavones derived from Saussurea medusa Maxim. for the HaCaT cells injury caused by UVB radiation
WENG Tengyu1 GUO Yan2
Objective To investigate the effects of flavones derived from Saussurea medusa Maxim. for the HaCaT cells injury caused by UVB radiation. Methods The HaCaT cells line cultured in vitro was randomly divided into two groups, which were respectively given UVB irradiation at doses of 25 mJ/cm2 and 50 mJ/cm2. Then, the cells were further subdivided into four groups, including control group, UVB model group, 2 g/L of flavones derived from Saussurea medusa Maxim. group, and 8 g/L of flavones derived from Saussurea medusa Maxim. group. The cell proliferation was determined by MTT assay. The activities of superoxide dismutase (SOD), contents of glutathione (GSH), malondialdehyde (MDA) and activities of catalase (CAT) were detected by enzyme linked immunosorbent assay. Results In different doses of UVB radiation groups, compared with control group, the absorbance of UVB model group was decreased, the activities of SOD, CAT and the contents of GSH were reduced, while the contents of MDA were increased, the differences were all statistically significant (P ＜ 0.05). Compared with UVB model group, the absorbance of flavones derived from Saussurea medusa Maxim. group was increased, the activities of SOD, CAT and the contents of GSH were enhanced, while the contents of MDA were decreased, the differences were all statistically significant (P ＜ 0.05). Conclusion The flavones derived from Saussurea medusa Maxim. have certain protective effects for the oxidative damage of HaCaT cells after UVB radiation
2018 Vol. 15 (1): 14-17 [Abstract] ( 269 ) HTML (1 KB)  PDF (698 KB)  ( 191 )
18 The effect of Probucol on diabetic nephropathy in type 2 diabetic rats
CHENG Lilan1 HUANG Jinhua2 MA Aijiang1 QI Shan1 JIN Sumin1 ZUO Songbo1
Objective To explore the effect of Probucol on diabetic nephropathy in type 2 diabetic rats. Methods Sixteen diabetic rats were divided into DM group and PB group, 8 rats in each group. 9 normal rats  were chosen as normal control group (NC group). The UMA, BUN, Scr, HOMA-IR, renal hypertrophy index, TG, TC, LDL-C, SOD, GSH-PX, MDA and Pathologic histology were selected as detected indicators. The effect of Probucol on diabetic nephropathy in type 2 diabetic rats were evaluated. Results In DM group, BUN, UMA, renal hypertrophy index, HOMA-IR, Scr, TG, TC, LDL-C, MDA were significantly higher than those of the NC group, SOD and GSH-PX were significantly lower than those of the NC group (P < 0.05 or P < 0.01). In PB group, BUN, Scr, UMA, TC, LDL-C, MDA were significantly lower than DM group, SOD and GSH-PX were significantly higher than DM group (P < 0.05or P < 0.01). The changes of renal pathological structure in PB group was significantly severer than DM group. Conclusion  Probucol may play a role in diabetic nephropathy.
2018 Vol. 15 (1): 18-21 [Abstract] ( 291 ) HTML (1 KB)  PDF (584 KB)  ( 179 )
22 Study on reporter gene assay for determination of the bioactivity of polyethylene glycol typeⅠinterferon
PEI Dening YU Lei GUO Ying SHI Xinchang RAO Chunming
Objective To investigate the applicability of reporter gene assay in Chinese Pharmacopoeia (2015 edition) for determination of the bioactivity of polyethylene glycol (PEG) type Ⅰ interferon. Methods According to ICH guidelines, specificity, linearity, accuracy (recovery) and precision (repeatability and intermediate precisions) of reporter gene assay were validated. The determination results of reporter gene assay and cytopathic inhibition assay were also compared. Results Application of reporter gene assay for determination of the bioactivity of PEG type Ⅰ interferon had good specificity, the dose-response of which was fitted to 4-parameter logistic model. The recovery rate was 85%-115%, the repeatability of the assay was 6.66%-7.70%. The intermediate precision of different dates, laboratory technicians and cell passages was 10.64%-11.01%, 13.04%-15.12% and 7.61%-8.54% respectively. The difference of the determination results between reporter gene assay and cytopathic inhibition assay was not statistically significant (P ＞ 0.05). Conclusion The reporter gene assay is applicable for bioactivity determination of PEG type Ⅰ interferon.
2018 Vol. 15 (1): 22-25 [Abstract] ( 351 ) HTML (1 KB)  PDF (737 KB)  ( 193 )
26 Population pharmacokinetics study of Omeprazole Enteric-Coated Capsules in Chinese healthy subjects
ZHAO Zheng SONG Boli LIU Yunxin ZOU Jianjun ZHU Yubing
Objective To establish population pharmacokinetics model of Omeprazole Enteric-Coated Capsules in Chinese healthy subjects, thus quantitatively evaluating the factors that could influence clinical pharmacokinetics of Omeprazole. Methods Twenty healthy male subjects recruited in Nanjing First Hospital from April 2016 to June 2016 were given 20 mg single dose of Omeprazole Enteric-Coated Capsules and plasma concentrations of the drug were determined by LC-MS/MS. CYP2C19 gene polymorphism was measured by polymerase chain reaction-restriction fragment length polymorphism method. The population pharmacokinetic model was established by nonlinear mixed effect model (NONMEM)program and estimated by bootstrap procedure. Results One-compartmen model of first-order elimination had fit for Omeprazole pharmacokinetics. Inter-individual variability was described by exponential model．The population typical values of CL/F,V/F and Ka were19.5L/h,16Land0.633/h. BUN had a significant influence on Ka. CYP2C19 phenotype showed significant effects on CL/F. The parameters estimated from bootstrap procedure were comparable to those from NONMEM. Conclusion The established population pharmacokinetics model can explain the individual pharmacokinetic variations of Omeprazole, which provides guidance for clinical personalized drug administration.
2018 Vol. 15 (1): 26-30 [Abstract] ( 355 ) HTML (1 KB)  PDF (808 KB)  ( 245 )
31 Preliminary treatment process containing inclusion compound of volatile oil
LI Chunhua LI Jiajia DUN Jiaying ZHENG Peng
Objective To screen the best pretreatment technology of volatile oil inclusion compound. Methods The research on factors and the type of organic solvent, solvent volume, extraction time, the content of cinnamic aldehyde as the score index, inclusion extracted by steam distillation method, through L9（34） orthogonal test, the optimum extraction process. Chromatographic conditions: temperature programmed: 90-180℃; injection volume: 1 μL; split ratio was 50:1. Results The optimum process was the extraction of n-butanol, the volume was 2 mL, and the extraction time was 2 hours. And the volume of solvent had significant influence on this experiment. Conclusion The optimum extraction process is proved to be scientific and feasible, and it can be widely used in inclusion complex study.
2018 Vol. 15 (1): 31-33,48 [Abstract] ( 273 ) HTML (1 KB)  PDF (938 KB)  ( 163 )
34 Effects of flavonoids extracted from Alternanthera philoxeroides on HBsAg and HBeAg expression content in HepG2. 2. 15 cells
WU Qian-hu1 XU Weidong2 LI Weidong3 XU Lu4 LING Mei1 WU Yuhua1
Objective To study the anti-HBV action of total flavonoids extracted from Alternanthera philoxeroides in vitro. Methods HepG2.2.15 cells were inoculated into the cell culture plates and then randomly divided into negative control group, experimental group and positive control group. The negative control group was treated with 100 mL/L DMSO, the experimental groups were treated with 800, 400, 200 and 100 mg/L total flavonoids extracted from Alternanthera philoxeroides. In the positive control groups, lamivudine was added at the concentration of 800, 400, 200 and 100 mg/L. Cell culture and optical density assay were used to study the effects of total flavonoids from Alternanthera philoxeroides on the expression of HBsAg and HBeAg in HepG2.2.15 cell line. Results The inhibit the expression of HBsAg in HepG2.2.15 cells in each experimental group was obviously higher, compared with the negative group, the difference was statistically significant (P < 0.01) and compared with the positive control group, the difference was statistically significant (P < 0.05), the inhibition rate of HBsAg in the experimental group was positively correlated with the dose of the drug. Each dose of the experimental groups had obvious inhibitory effects on the expressions of HBeAg in HepG2.2.15 cells, compared with the negative control group, the difference was statistically significant (P < 0.01) compared with and positive control group, the difference was statistically significant (P < 0.05), the inhibition rate of HBeAg in the experimental group was positively correlated with the dose of the drug. Conclusion Total flavonoids extracted from Alternanthera philoxeroides has obvious inhibitory effects on the expressions of HBsAg and HBeAg in HepG2. 2. 15 cells. Total flavonoids extracted from Alternanthera philoxeroides has strong anti-HBV action in vitro.
2018 Vol. 15 (1): 34-37 [Abstract] ( 296 ) HTML (1 KB)  PDF (649 KB)  ( 139 )
38 Study on optimization and stability of Emu Oil microemulsion prescription
LAN Xiaoqun1 SHEN Xiao2 YUN Xian1 WANG Lisheng2
Objective To prepare the Emu oil Microemulsion and investigate the stability of the preparation. Methods The Emu oil Microemulsion was prepared by adding water and the prescription process was optimized by single factor method and orthogonal test. The appearance shape score, the centrifugal stability constant and the particle size were selected as the index, the Emu oil Microemulsion was prepared by screening the ratio of Tween 80/Span80 and the amount of glycerol and the stability of the preparation prepared by the optimal formulation was investigated. Results The optimal Microemulsion was composed of emu oil 0.03 g, Tween80/Span80 （4∶1） 0.80 g, glycerol 0.20 g and plused deionized water to 20 mL. The prepared Microemulsion was O/W type, which was clear and transparent, the average particle size of 100-200 nm and there is no separation after the separation and oil droplets. Conclusion The Emu oil Microemulsion made by this optimum formulation and preparation procedure has excellent physicochemical properties.
2018 Vol. 15 (1): 38-41 [Abstract] ( 309 ) HTML (1 KB)  PDF (691 KB)  ( 289 )
42 Effect of HPMC E3 on the improvement of melting wall sticking of Fructus Jujubae extractum in spray drying
DU Zhongmei
Objective To study the effect of hypromellose E3 （HPMC E3） on the melting wall sticking of Fructus Jujubae Extractum during spray drying. Methods A certain percentage of HPMC E3 were added to the total Fructus Jujubae Extractum to conduct the spray drying. The effect of HPMC E3 against the wall sticking effect of the spray drying was studied. Moreover, the  spray drying parameters were investigated. The optimized effect was evaluated by the characterization of the critical physical attributes of the spray drying powder. Results The best formula consisted of 8% HPMC E3, and the optimal spray-drying parameters were composed of inlet air temperature of 120℃,1.05 bar atomization pressure, and 4.5 mL/min pump speed, respectively. Conclusion HPMC E3 is a vital role in improving the melting wall sticking of Fructus Jujubae Extractum.
2018 Vol. 15 (1): 42-44,63 [Abstract] ( 255 ) HTML (1 KB)  PDF (1049 KB)  ( 158 )
45 Research progress on the mechanism of osteopontin and kidney stone formation
XIA Yuqi CHENG Fan YUAN Run NING Jinzhuo
OPN is one of the main components of the organic phase of stone, which is highly expressed in the renal tissue of the kidney stones patients and animal stone models, and plays an important role in the process of crystal aggregation and adhesion, oxidative stress of renal tubular epithelial cells and apoptosis. A large amount of OPN expression will lead to crystal deposition, stone formation, resulting in kidney cell damage. OPN antibodies, antioxidants and apoptotic pathway inhibitors can significantly reduce OPN mediated stone formation. This review aims to discuss recent advances of the role of osteopontin in the formation of kidney stones and the new stone prevention and treatment target for OPN.
2018 Vol. 15 (1): 45-48 [Abstract] ( 417 ) HTML (1 KB)  PDF (354 KB)  ( 311 )
49 The differences of Xpert MTB/RIF and BACTEC MGIT960 in detecting Mycobacterium tuberculosis and Rifampin resistance
CHEN Junlin1 HUANG Fei1 XU Feifan1 GU Delin1▲ HU Zhongyi2
Objective To explore the differences between Xpert MTB/RIF and BACTEC MGIT960 in detecting Mycobacterium tuberculosis (MTB) and Rifampin (RFP) resistance. Methods From June 2014 to June 2017, sputum specimens from 188 retreated smear positive pulmonary tuberculosis patients in the Sixth People's Hospital of Nantong were selected as research objects. Xpert MTB/RIF and BACTEC MGIT960 were used to detect MTB in the sputum specimens of the patients and the RFP resistance was analyzed combined with the medical history. Results Then were 146 cases of consistency results between Xpert MTB/RIF and BACTEC MGIT960 in detecting MTB, while 38 cases of heterogeneity. The number of cases in Xpert MTB/RIF group was higher than that of BACTEC MGIT960 group in the use of the drug with rifamycin and the duration above 3 months, with statistically significant difference (χ2=6.31，P ＜ 0.05). There were no statistically significant differences between Xpert MTB/RIF and BACTEC MGIT960 in the comparison of other medical history groups (P ＞ 0.05). Conlusion The results of Xpert MTB/RIF and BACTEC MGIT960 in detecting MTB and RFP resistance from sputum specimens of smear positive are differential. It may be associated with medical history of the patients. Clinical physicians should pay attention to the differences of the two detection methods and formulate anti-tuberculosis treatment plan accurately.
2018 Vol. 15 (1): 49-52 [Abstract] ( 308 ) HTML (1 KB)  PDF (675 KB)  ( 164 )
53 Effect of BAL on bronchiectasia with infection and its effects on blood gas and serum inflammatory factors
Miriguli·Yilihamu LU Yao WANG Hui Sawuti·Tuerdi
Objective To investigate the effect of bronchoalveolar lavage (BAL) on bronchiectasia(BE) with infection and its influence on the levers of blood gas indexes and serum inflammatory indexes. Methods From January 2015 to December 2016, 120 patients came from the First People′s Hospital of Aksu for treatment of BE with infection were selected as the study object. They were divided into groups by random number table. 60 patients of control group were treated with basic treatment measures, 60 patients of treatment group were treated with BAL under fiberoptic bronchoscope on the basis treatment of the control group. The blood gas indexes and serum inflammatory indexes of the two groups were measured before and after treatment, and the clinical efficacy was evaluated at the end of the course. The blood gas index, serum inflammatory index and clinical effective rate were compared between the two groups before and after treatment. Results Before treatment, the levels of arterial partial pressure of oxygen (PaO2), arterial partial pressure of carbon dioxide (PaCO2) and ]oxygenation index (PaO2/FiO2) in the two groups were not statistically significant (P > 0.05); after treatment, the levels of PaO2 and PaO2/FiO2 in the treatment group were higher than those in the control group (P < 0.05), and PaCO2 was lower than that in the control group (P < 0.05). Before treatment, the levels of TNF-α, Hypersensitivity C reactive protein (hs-CRP), Interleukin -2 (IL-2), IL-4, IL-23 and transforming growth factor-β (TGF-β) in the treatment group and the control group were not statistically significant (P > 0.05); After treatment, the level of IL-2 in the treatment group was higher than that in the control group (P < 0.05), and the levels of TNF-α, hs-CRP, IL-4, IL-23 and TGF-β in the treatment group were lower than those in the control group (P < 0.05). After treatment, the effective rate of the treatment group was 58.33%, which was higher than control group 41.67% (P < 0.05). Conclusion Serum inflammatory factor levels and blood gas level of BE and infection can be improved after BAL treatment, it is worthy of clinical application.
2018 Vol. 15 (1): 53-56 [Abstract] ( 308 ) HTML (1 KB)  PDF (660 KB)  ( 105 )
57 Clinical significance of indocyanine green clearance test in the diagnosis of hepatitis B liver cirrhosis
GUO Lu CHEN Xiaoli WANG Dexin YAN Xiuping
Objective To study the clinical significance of indocyanine green excretion test in the diagnosis of patients with hepatic B liver cirrhosis. Methods The clinical data of 275 patients with hepatitis B (chronic hepatitis B group) and 306 patients with hepatic B liver cirrhosis (hepatitis B liver cirrhosis group) in the Sixth People's Hospital of Qingdao from June 2016 to July 2017 was analyzed retrospectively. The indole cyanogen green (ICG) discharge tested, liver stiffness detection and abdominal ultrasonography were carried out in the two groups. Results Compared with chronic hepatitis B group, T1/2,R15 value and E value was increased in hepatic B liver cirrhosis group, and the K value was decreased, the differences were statistically significant (P < 0.05). K value and CAP value were negatively correlated with the degree of liver sclerosis (r = -0.672, -0.826, all P < 0.05), R15 value was positively correlated with the degree of liver sclerosis (r = 0.891, P < 0.05). Conclusion Indocyanine green test is helpful to evaluate the liver sclerosis, as a supplement to Fibroscan, and can be the new indication and method to investigate the degree of liver sclerosis.
2018 Vol. 15 (1): 57-59,94 [Abstract] ( 272 ) HTML (1 KB)  PDF (1129 KB)  ( 234 )
60 Analysis of influence factors of successful offline extraction of patients with mechanical ventilation in ICU
TIAN Yueming1 ZOU Min1 ZHANG Qiqi2
Objective To explore the influence factors of the successful removal of the mechanical ventilation patients in intensive care unit (ICU). Method 89 cases of patients with COPD admitted in ICU of Rocket Army General from March 2013 to January 2017 were retrospectively analyzed. The various basic information of all patients was collected, and the offline tube drawing of patients with mechanical ventilation in ICU was analyzed. At the same time the influence factors of successful decannulation weaning of patients with mechanical ventilation were detected by using single factor analysis and multivariable Logistic regression analysis method in turn. Results The successful off-line extraction rate of 89 cases of patients with mechanical ventilation was 59.55% (53/89), which was treated as the successful group, and 36 cases of patients of failure of the extractor and the death were treated as the failure group; The single factor and multiple factors Logistic regression analysis showed that age (≥50 years), ventilation time (≥7 days), APACHE Ⅱ (≥30 points), presence of infection, ALB (<35 g/L) and the existence of bad psychology was influence factors ofsuccessfully weaning tube drawing of patients in ICU. Conclusion The offline decannulation rate of patients with mechanical ventilation in ICU isn′t high, of which age, aeration time, APACHE Ⅱ, infection, propagated and unhealthy emotion are the influence factors of offline tube drawing of the patients with mechanical ventilation in ICU. Pay attention to the clinical risk factors improves mechanical ventilation weaning decannulation rate.
2018 Vol. 15 (1): 60-63 [Abstract] ( 364 ) HTML (1 KB)  PDF (631 KB)  ( 116 )
64 The expression of galectin-3 in serum of patients with egg allergy and its correlation with egg-specific IgE
ZHANG Shuchen YANG Lin HU Lintao YANG Yongshi ZHU Rongfei
Objective To detect the expression of galectin-3(Gal3) in serum of patients with egg allergy and the correlation with egg-specific IgE. Methods Screening 68 patients with egg allergy according to the history of allergy, prick test, egg-specific IgE and food exclusion from April 2016 to April 2017 in Department of Allergy of Tongji hospital Affiliated to Tongji Medical College of Huazhong University of Science & Technology. The patients with egg allergymanifested withanaphylactic shock, eczema, chronic urticaria, mild dermatitis, allergic asthma, cough variant asthma and allergic rhinitis were selected as egg allergy group. Twenty patients with no history of allergies, skin prick test and egg serum specific IgE were negative were selected as healthy controlsin the same period. The correlation between Gal3 expression and egg-specific IgE in patients with different symptoms was analyzed. Results The average concentration of Gal3 in serum of control group was (4.23±0.53) ng/mL, the average concentration of Gal3 in serum of egg allergy group was(9.95±6.31)ng/mL. The difference was statistically significant (P = 0.0032). The expression of Gal3 in each group: the concentration of Gal3 in anaphylactic shock group was (17.11±2.54) ng/mL and allergic asthma was (10.95±1.97) ng/mL significant higher than those of control group, the difference was statistically significant (P= 0.0007, 0.0001); the group of chronic urticaria was (6.71±2.13) ng/mL and allergic rhinitis was(7.24±1.75) ng/mL, compared with the control group, the differences were statistically significant (P = 0.038, 0.022); the eczema group (5.04±0.15) ng/mL, mild dermatitis group was(4.24±1.33) ng/mL and cough variant asthma group was (3.24±2.75) ng/mL, the differences were not statistically significant(P = 0.156, 0.354, 0.282 respectively). The expression of Gal3 was positively correlated with egg-specific IgE in serum (r = 0.5932). There was a positive correlation among allergic asthma, allergic rhinitis and cough variant asthma in patients with egg-specific IgE (r = 0.7792, 0.8658, 0.8091, all P < 0.01), but there was no significant difference between Gal3 expression and egg-specific IgE in the other symptoms (P > 0.05). Conclusion The expression of Gal3 in serum of patients with allergic asthma, allergic rhinitis and chronic urticaria was significantly increased, which can prompt allergy patients provoked by egg.
2018 Vol. 15 (1): 64-67 [Abstract] ( 366 ) HTML (1 KB)  PDF (760 KB)  ( 169 )
68 Expression level and clinical value of serum growth differentiation factor -15 and C reactive protein in chronic obstructive pulmonary disease
WANG Shanquan1 LIU Chengzhou1 LIU Tongxiang2
Objective To observe the expression level and clinical significance of serum growth differentiation factor-15 and C reactive protein in chronic obstructive pulmonary disease. Methods From February 2015 to May 2017 in 239 cases of COPD patients in Linqu People's Hospital of Shandong Province. Grouped according to the patient's condition: 136 stable patients, 103 cases of patients in acute stage at the same time, 80 healthy volunteers were selected as control group. All patients were selected for determination of serum growth differentiation factor -15 and C reactive protein, the expression levels of the above indexes in the three groups were analyzed. The ROC curve was used to analyze the sensitivity and specificity of the above indexes in the diagnosis of COPD. Results Growth differentiation factor-15 and C reactive protein in the control group, stable COPD group, acute COPD group were significantly increased, the difference between the three groups are statistically significant (P < 0.01). The area of growth differentiation factor-15 in the diagnosis of acute COPD curve was 0.79 (95%CI: 0.61-0.89, P < 0.01), the diagnostic accuracy was 72%. The area under the curve of C reactive protein in the diagnosis of acute COPD was 0.66 (95%CI: 0.51-0.76, P < 0.01), and the diagnostic accuracy was 61%. Conclusion The expression levels of GDF-15 and CRP in patients with CRP are significantly increased, and positively correlated with the severity of the disease, the accuracy of GDF-15 in the diagnosis of COPD is higher than that of CRP.
2018 Vol. 15 (1): 68-71 [Abstract] ( 241 ) HTML (1 KB)  PDF (726 KB)  ( 129 )
72 Clinical role of monitoring of oxygenation index and alveolar arterial oxygen partial pressure difference in children with severe pneumonia
YANG Yi′nan WANG Haiyan Chaolumen Qiqige
Objective To explore the clinical role of monitoring of oxygenation index (OI) and Alveolar arterial oxygen partial pressure difference (A-aDO2) for children with severe pneumonia. Methods The clinical data of 71 children with severe pneumonia who had been seeking treatment in the Affiliated Hospital of Inner Mongolia Medical University from March 2016 and March 2017 were set as the study group, and according to the acute physiology and chronic health evaluation (APACHE-Ⅱ) scores, these patients were divided into study group A (≥40 points), the study group B (20 -＜40 points) and study group C (<20 points). Another 71 healthy children were selected during the same period as the control group. Blood gas monitoring was carried in all the three groups. The OI/endothelial surface area, A-aDO2/endothelial surface area at difference time periods, and prognosis in the three study groups were compared. Results The OI/endothelial surface area was registered at in study group A, in study group B and in study group C, all lower than the control group, and the study group A was the lowest, where the differences were statistically significant (P < 0.01). The A-aDO2/endothelial surface area in study group A, study group B, and study group C were higher than that in the control group, and the study group A was the highest (P ＜ 0.01). The OI/endothelial surface area increased over the treatment time, whereas the A-aDO2/endothelial surface area decreased over the treatment time. All of the above differences were statistically significant (P < 0.01). Out of the 71 cases of children with severe pneumonia, 70 cases survived and 1 died. Conclusion For children with severe pneumonia, dynamic monitoring of OI and A-aDO2 can assess their conditions, thus providing guidance for its clinical treatment and prognosis improvement. Therefore, it is of significant value to be popularized in clinical application.
2018 Vol. 15 (1): 72-75 [Abstract] ( 378 ) HTML (1 KB)  PDF (620 KB)  ( 135 )
76 Clinical observation and effect of Salvia Tetramethylpyrazine Injection on serum homocysteine, high-sensitivity C-reactive protein and lipid levels in ischemic stroke
CHEN Guoning LIN Zehui DU Yongming Li Quan
Objective To explore the effect of Salvia Tetramethylpyrazine Injection on serum homocysteine, high-sensitivity C-reactive protein and lipid levels in ischemic stroke and clinical efficacy. Methods From February 2014 to November 2016, 100 patients with ischemic stroke in the emergency department of Chinese Medicine Hospital of Hainan Province, Guangzhou University of Chinese Medicine were selected and divided into treatment group and control group according to therapeutic schedule, with 50 cases in each group. The control group was treated with conventional treatment, while the treatment group was treated Salvia Tetramethylpyrazine Injection on the basis of control group. The serum homocysteine, high-sensitivity C-reactive protein, lipid levels, clinical efficacy, NIHSS score of the two groups were observed. Results After treatment, the levels of serum homocysteine, high-sensitivity C-reactive protein , total cholesterol, triacylglycerol and lipoprotein (a) in the treatment group were lower than those in the control group, with statistically significant difference ( P ＜ 0.05). The NIHSS score and clinical efficacy of treatment group were significantly better than those of control group, with statistically significant difference (P ＜ 0.05). Conclusion Salvia Tetramethylpyrazine Injection can reduce the serum homocysteine, high sensitivity c-reactive protein and serum lipid levels in patients with ischemic stroke, improve the clinical efficacy and neurological function．
2018 Vol. 15 (1): 76-79 [Abstract] ( 654 ) HTML (1 KB)  PDF (618 KB)  ( 161 )
80 Clinical application of strengthening Yang guidance in the cardiac rehabilitation
LI Haixia1 LI Jun1 LI Ruijie2▲
Yang plays an irreplaceable role in the body, strengthening Yang is essential for the treatment and rehabilitation of disease. Daoyin is the key medicine of strengthening Yang that accorded with moving produces Yang, happiness produces Yang and kindness produces Yang. Cardiac rehabilitation attaches great importance to exercise therapy. As a rehabilitation exercise therapy under the guidance of TCM theory, Daoyinhas a long history and a variety of forms. Daoyin is different from other sports. Daoyin of strengthening Yang emphasizes the combination of static and dynamic, pays attention to regulating body, heart and breath. Movement mode of Daoyin is that repairing the circle of Yang, balancing Yin and Yang. It has positive effect on prevention and adjuvant treatment of cardiovascular disease, and it has advantages in the rehabilitation treatment of heart diseases. Gymnastic qigong is a kind of Daoyin which combines with body movement, breathing and mental adjustment,it has the characteristics of low intensity. There are eight frequently-used gymnastic qigong: Baduanjin, six words, Tai Chi, MawangduiDaoyin, Wuqinxi, muscle-bone strengthening exercise, dance and Tai Chi sticketc. They have different characteristics, are suitable for acute stage, recovery period and consolidation phase of cardiac rehabilitation. Gymnastic qigong is safe and suitable for clinical cardiac rehabilitation, and it is conducive to patients with systemic rehabilitation.
2018 Vol. 15 (1): 80-85 [Abstract] ( 342 ) HTML (1 KB)  PDF (723 KB)  ( 279 )
86 Study on thermal structure characteristics of phlegm dampness constitution in young people
WANG Yuting1 DENG Pin1 XIANG Lekang2 LI Hongjuan2
Objective To study differences of caloricity data ΔT in 25 regions including viscera, head and face among youth people (aged from 15 to 34 years old) who have Phlegm-dampness constitution and mild physical to compare thermal structure of the three visceral cavities, viscera, Ren and Du meridians and face and head and five sense organs. Methods Classification and determination of constitution in TCM was adopted with combination of TCM four diagnostic methods. Thirty cases for Phlegm-dampness constitution group and thirty cases for mild physical group were chosen from healthy youth from TCM Clinic, Beijing University of Chinese Medicine and Beijing University of Chinese Medicine, Liangxiang Campus from May to September 2016. Infrared thermopraphy was taken to obtain infrared thermal value of three visceral cavities, viscera, Ren and Du meridian and face and head and five sense organs. Results Compared with mild physical group, both Ren and Du meridians in the Phlegm-dampness constitution group indicated cool departure (P < 0.05), upper-jiao heat departure (P < 0.01), middle jiao and lower jiao cool departure (P < 0.01), anterior chest heat departure (P < 0.05), upper abdomen and lower abdomen cool departure (P < 0.01) and nose cool departure (P < 0.05). Conclusion Thermal structure for youth people with Phlegm-dampness constitution features much yin and less yang inside their body and weak spleen and stomach. It is also characterized by the tendency of dampness and heat accumulation.
2018 Vol. 15 (1): 86-89 [Abstract] ( 245 ) HTML (1 KB)  PDF (670 KB)  ( 114 )
90 Study on the medication rules of traditional Chinese medicine in the treatment of psoriasis based on ancient literatures
LI Yang PEI Li
Objective To explore the medication rules of traditional Chinese medicine in the treatment of psoriasis in the ancient medical literatures of China, and to provide reference for the treatment of contemporary psoriasis. Methods The contents of traditional Chinese medicine in the treatment of psoriasis in ancient literatures were collected and sorted. The data mining technology was used to analyze the frequency, type and frequency of drug properties of psoriasis treatment, and 51 high frequency drugs were taken clustering analysis through SPSS software. Results This paper showed that the drugs involved in the treatment of psoriasis in the ancient medical literatures of China were 294 flavors, of which, high frequency drugs were 51 flavors. The kinds of 294 drugs were mainly heat-clearing drugs, superficies-relieving drugs, expelling superficial evils and destroying parasites drugs, restoring vital energy drugs, accounted for 50% of all drugs. The drug properties and pharmaceutical tastes were mainly warm, cold, calm and bitter, pungent, sweet. Conclusion This paper summarizes the medication rules of psoriasis in ancient China, reveals the characteristics of traditional Chinese medicine in the treatment of psoriasis, and provides reference and basis for clinical research of dominant diseases of traditional Chinese medicine.
2018 Vol. 15 (1): 90-94 [Abstract] ( 305 ) HTML (1 KB)  PDF (873 KB)  ( 86 )
95 Single case randomized controlled research of Modified Huangqi Chifeng Decoction in the treatment of proteinuria due to IgA nephropathy
JIAO Zhina ZHAO Mingming ZHANG Yu▲ LI Liusheng SI Yuan
Objective To observe the efficacy and safety of Modified Huangqi Chifeng Decoction in the treatment of proteinuria due to IgA nephropathy. Methods The cases came from the Outpatient Department of Nephrology in Xiyuan Hospital, China Academy of Chinese Medical Sciences from December 2011 to December 2012. 5 cases of patients with IgA nephropathy with mild to moderate proteinuria, normal kidney function and syndrome of blood stasis due to qi deficiency were enrolled. By using single case randomized controlled design, Modified Huangqi Chifeng Decoction combined with Telmisartan was applied in the treatment period and single Telmisartan was applied in the control period. The efficacy indices were 24-hour urine protein quantification and TCM symptom scores, the safety indices were liver and kidney function and blood routine examination. Results The TCM symptom scores of each case in the treatment period were significantly reduced in different degrees compared with those of control period (P ＜ 0.05); after TCM symptom scores of 5 patients were integrated, the treatment period was significantly improved compared with those of control period (P ＜ 0.05). The 24-hour urine protein quantification of each patient in the treatment period was significantly lower than those of control period (P ＜ 0.05), after 24-hour urine protein quantification of 5 patients was integrated, the treatment period was significantly less than that of control period (P ＜ 0.05). Conclusion Modified Huangqi Chifeng Decoction in treatment of proteinuria due to IgA nephropathy is safe and effective, and it is feasible to use single case randomized control design in clinical research of TCM.
2018 Vol. 15 (1): 95-98 [Abstract] ( 291 ) HTML (1 KB)  PDF (708 KB)  ( 108 )
99 The experience of Professor LU Min in treating diarrhea predominant irritable bowel syndrome
KE Shuifang1 LU Min2
Irritable bowel syndrome (IBS) is a kind of functional intestinal disorder, which, characterized by diarrhea, is called diarrhea predominant irritable bowel syndrome (IBS-D). Professor LU Min believes that the causes of IBS-D are aeipathia lost treatment, affect-mind dissatisfaction and improper diet, which tend to liver dysfunction, spleen deficient, thus lead to liver qi invading spleen. In the clinical work, the method of supporting the spleen and restricting the liver supplemented by restoring normal coordination between heart and kidney is used to treat IBS-D and has obtained a remarkable therapeutic efficacy.
2018 Vol. 15 (1): 99-101,105 [Abstract] ( 275 ) HTML (1 KB)  PDF (691 KB)  ( 136 )
102 Changes and significances of platelet parameters in hepatocellular carcinoma patients with different syndrome classified by traditional Chinese medicine
OUYANG Bohui XIE Qingqing TANG Jian SUN Yifan▲
Objective To studythe changes and significances of platelet distribution width(PDW)and mean platelet volume (MPV) in hepatocellular carcinoma (HCC) patients with different syndrome classified by traditional Chinese Medicine(TCM). Methods A retrospective study was carried out to analyze the clinical data of 155 HCC patients from January 2013 to December 2016 in Liuzhou Hospital of Traditional Chinese Medicine. All patients was divided into four groups according to TCM syndrome: stagnation of liver-Qi, qi-stagnation and blood stasis, dampness and heat accumulation, stagnation of liver-Qi with deficiency of the spleen.The differences of PDW and MPV among different groups were analyzed. Results The level of PDW in stagnation of liver-Qi group were significantly higher than that of the other groups (P < 0.01), however, the level of MPV in stagnation of liver-Qi group was significantly lower than that of the other groups (P < 0.01). Compared with qi-stagnation and blood stasis and dampness and heat accumulation group, the level of PDW increased significantly in stagnation of liver-Qi with deficiency of the spleen group(P < 0.01), and the level of MPV in the stagnation of liver-Qi with deficiency of the spleen group were significant higher than that of the other three groups (P < 0.01). Conclusion The PDW and MPV can be used as important reference indexes for HCC patients with stagnation of liver-Qi and syndrome of stagnation of liver-Qi with deficiency of the spleen in primary
2018 Vol. 15 (1): 102-105 [Abstract] ( 289 ) HTML (1 KB)  PDF (708 KB)  ( 129 )
106 The effect of scalp acupuncture therapy combined with synchronous MOTOmed training on lower limb function in patients with stroke
GUO Yangyang LI Yi
Objective To observe the effect of scalp acupuncture therapy combined with synchronous MOTOmed training on lower limb function of hemiplegic patients after stroke. Methods Sixty patients with cerebral apoplexy admitted to Department of Rehabilitation, Affiliated hospital of Zunyi Medical University from January 2016 to January 2017 were selected and divided into observation group and control group by random number table method, with 30 cases in each group. In the observation group, the MOTOmed training was applied during scalp acupuncture treatment. In the control group, the scalp acupuncture was done in the morning and the MOTOmed training was applied in the afternoon. The efficacy was evaluated by national institute of health stroke scale (NIHSS), Fugl-Meyer assessment (FMA), Berg balance scale (BBS) and modified Barthel index (MBI) before and in eight weeks of treatment, respectively. Results The NIHSS scores of the two groups after treatment were lower than those before treatment (P ＜ 0.05), and the NIHSS scores of observation group after treatment were lower than those of control group, the differences were statistically significant (P ＜ 0.05). The FMA, BBS and MBI scores of the two groups after treatment were higher than those before treatment (P ＜ 0.05), and the FMA, BBS and MBI scores of observation group after treatment were higher than those of control group, the differences were statistically significant (P ＜ 0.05). Conclusion For the hemiplegic patients after stroke, the model of scalp acupuncture therapy combined with synchronous MOTOmed training achieves the superior improvements of the lower limb motor function, balance function and the activity of daily life as compared with non-synchronous model.
2018 Vol. 15 (1): 106-108,126 [Abstract] ( 273 ) HTML (1 KB)  PDF (1132 KB)  ( 234 )
109 Meta-analysis of combined treatment with oral Finasteride and topical Minoxidil in male androgenetic alopecia
ZHANG Shudan1 LI Qi2
Objective To review systematically the curative effect and safety of combined treatment with oral Finasteride and topical Minoxidil in male androgenetic alopecia (AGA). Methods The databases of PubMed, EMbase, Cochrane Library, CBM, WanFang, CNKI and VIP were retrieved for collecting RCT and CCT about combined treatment with oral Finasteride and topical Minoxidil in AGA before March 20th 2017, and reference lists were also searched. According to the inclusion and exclusion criteria, two reviewers screened and assessed independently the potential studies, and then the data was extracted. The data was given a Meta-analysis by using RevMan 5.3 software. Results Seven study articles were included, involving 1786 patients. The Meta-analysis showed: after 3 months of treatment, the clinical curative effect of the combination group in treating AGA was better than the Minoxidil group (OR = 1.95, 95%CI: 1.37-2.76, P = 0.0002) and the Finasteride group (OR = 2.30, 95%CI: 1.64-3.23, P ＜ 0.000 01). After 6 months of treatment, the clinical curative effect of the combination group in treating AGA was better than the Minoxidil group (OR = 2.20, 95%CI: 1.26-3.84, P = 0.005) and the Finasteride group (OR = 2.50, 95%CI: 1.46-4.30, P = 0.0009). After 12 months of treatment, the clinical curative effect of the combination group in treating AGA was better than the Minoxidil group (OR = 6.83, 95%CI: 2.92-15.99, P < 0.000 01) and the Finasteride group (OR = 2.77, 95%CI: 1.77-4.32, P ＜ 0.000 01). There were no serious adverse reactions in each group. Conclusion The combination treatment of Finasteride and topical Minoxidil has significant curative effect,  and the safety is good.
2018 Vol. 15 (1): 109-113,149 [Abstract] ( 392 ) HTML (1 KB)  PDF (1305 KB)  ( 197 )
114 The effect of chest CT scan of different dose on the patients with pacemaker implantation
XU Kai1 ZHAO Cunrui2 ZHANG Huijun1 SUN Mu1 XIANG Zongxing1 CHEN Rufei1 WANG Anxing1▲
Objective To explore the effect of chest CT scan of different dose on the patients with pacemaker implantation. Methods The clinical data of 152 patients with pacemaker implantation with CT coronary angiography in Baoji Hospital of Traditional Chinese Medicine from August 2015 to August 2017 were analyzed retrospectively. According to the different dose for CT scan , the patients were divided into conventional dose group (76 cases) and low dose group (76 cases). All used the OPTIMA CT660 scanners of 64 row 128 layers produced by GE, the parameters of conventional dose group were set of voltage 120 V, CARE Dose 4D, current 150 mA, pitch 1.0, layer thickness 5.0 mm, layer distance 5.0 mm, impact matrix 512×512. The parameters of low dose group were set of except current 30 mA, the remaining parameters were as same as the conventional dose group. The image quality and radiation dose of the two groups were compared. Results The pulmonary image and mediastinum window image in conventional dose group showed good blood vessels without metal artifacts, the surrounding tissue was clear. The pulmonary images and mediastinum window image in the low dose group showed that with mild metal artifacts, edges were fuzzy, and still visible from the surrounding tissue. There was no significant difference in the vascular distribution of CT image quality scores between the two groups (P > 0.05). CT dose irldex (CT- DI), dose-length product (DLP) and effective dose (ED) were compared between the two groups, the low dose group were lower than those in the conventional dose group, with statistically significant difference (P < 0.05). Conclusion For the pacemaker implantation patients with chest CT scans, the conventional dose and low dose of CT scans can provide excellent image quality. In the case of approximately the same case data, the low dose chest CT scan in patients with pacemaker implantation has a wider heart rate applicability compared to the conventional dose, which is worth of clinical application.
2018 Vol. 15 (1): 114-117 [Abstract] ( 409 ) HTML (1 KB)  PDF (461 KB)  ( 156 )
118 Diagnostic value of galacto-mannan (GM) test of bronchoalveolar lavage fluid for invasive fungal infections
SHAN Yu LIU Junquan▲ ZHU Yuqin LIAO Yuqin
Objective To analyze the correlation between the results of galacto-mannan (GM) test in bronchoalveolar fluid (BALF) and the positive results of invasive pulmonary aspergillus. Methods From January 2014 to December 2016, 8086 BALF and 1459 serum from patients with respiratory disease in 650 cooperative medical institutions (including tertiary, secondary and community hospitals) in Zhejiang Province were collected. By using the GM test kits to carry out GM experiments in BALF and serum samples according to the instructions of the kit, the diagnostic value of the invasive pulmonary aspergillosis (IPA) was analyzed by the results of the I value, the IPA diagnosis compliance rate was analyzed with BALF results. Fungal culture was carried out in a conventional manner by using sand-fortified medium. Aspergillus identification: the fungal from the positive samples were cultured, so they could be subjected to 10% KOH based smear before microscopic observation, and then the Aspergillus were differentiated by their morphology and color. Results There were 1193 Aspergillus positive specimens among these 8086 BALF samples. In the Aspergillus positive samples, according to the results of I value of GM test: <0.5 for 61 cases (5.11%), 0.5 to 1 for 98 (8.21%), 1.1-2.0 for 164 (9.56%) and ＞2.1 for 870 (77.12%); and in 5103 cases which had no fungal growth, the results of I value of GM test was: <0.5 for 3623 cases (70.99%), 0.5-1 for 827 cases (16.21%), 1.1-2.0 for 376 cases (7.37%) and >2.1 for 277 cases. There was a significant difference between these two groups (P ＜ 0.01). There also was a significant difference between the Aspergillus positive group and the Candida positive group (P ＜ 0.01). The analysis of I value of GM test in BALF and invasive pulmonary aspergillosis (IPA) diagnostic coincidence rate: When the I value was set to ≥2.1, the sensitivity, specificity, positive predictive value and negative predictive value of IPA diagnosis were as follows: 77.12% 94.57%, 76.86% and 76.85%, the differences were statistically significant (P ＜ 0.01); with the analysis thatwhen I value was set to be≥0.5 for positive results (94.89%, 9.00%, 43.34% and 43.34%, respectively). The results of GM test in serum showed that the I value of the Aspergillus positive group was 0.5.63%, and the growth without fungal was only 6.54%, the differences were statistically significant (P ＜ 0.01). Conclusion When the I value is set to ≥2.1, the result of the GM test in BALF is significantly more valuable. When the I value isset to ≥0.5, the I value of GM test in serumis more appropriate.
2018 Vol. 15 (1): 118-122 [Abstract] ( 248 ) HTML (1 KB)  PDF (392 KB)  ( 229 )
123 Effect and practice of case three-dimensional teaching method in clinical practice teaching of international medical students
LIU Yang1 LI Li1 SUN Jin2 ZHENG Aijun1 LI1 Xinxin1 ZHOU Gang1
Objective To study and summarize the effect of casethree-dimensional teaching method in clinical practice teaching for international medical students. Methods International medical students at grade 2011 of China Three Gorges University and arranged to practice at Yichang Central People′s Hospital were selected. A total of 108 students were randomly divided into two groups, problem-based learning method was carried in the control group (54 students), including 28 men and 26 women; the case three-dimensional teaching method was carried in the experimental group (54 students), including 32 men and 22 women. At the end of the semester, the two groups were evaluated by comprehensive assessment and questionnaire survey. Results Comparison of the teaching results in two groups, the collection and writing of medical case, comprehensive written in the experimental group were higher than the control group, the differences were statistically significant（P < 0.05）; the satisfaction rate in the experimental group was also higher than the control group, the difference was statistically significant （P < 0.05）. Conclusion Case three-dimensional teaching method not only improves the international medical students′ case records collection and writing, comprehensive written examination results, but also to meet the needs of students for clinical probation study, effectively improve the quality of clinical practice of international medical students.
2018 Vol. 15 (1): 123-126 [Abstract] ( 262 ) HTML (1 KB)  PDF (652 KB)  ( 146 )
127 Status quo and correlation analysis of self-efficacy and professional identity of nursing students
Parida·Muhamment SUN Junyu WU Yunyun SHU Jiaojiao
Objective To investigate the status quo of self-efficacy and professional identity of nursing students and explore their relationship, and to provide reference for nursing educator to improve nursing students′ self-efficacy and professional identity. Methods By convenient sampling method a total of 1263 nursing students from the Xinjiang Medical University, Shihezi University and Modern Vocational Technical College in Xinjiang were surveyed by self-efficacy comprehensive scale and professional identity scale from October 2015 to June 2016. Results The nursing students′  total score of self-efficacy was (119.79±17.95) scores. The nursing students′ score of professional identity was (119.30±21.69) scores. By single factor analysis, the factors affecting the self-efficacy were gender, college entrance examination, nationality, and educational background. The difference was statistically significant (P < 0.05). There was a positive correlation between self-efficacy and occupational identity (r = 0.540, P < 0.01). By multifactor analysis, the change of influencing factors could lead to 39.2% of the change of occupational identification of nursing students. Conclusion Nursing students′ self-efficacy is an important factor of professional identity. The nursing educators should be based on the different characteristics of nursing students to take scientific and feasible interventions to improve the self-efficacy and professional identity.
2018 Vol. 15 (1): 127-130,135 [Abstract] ( 366 ) HTML (1 KB)  PDF (662 KB)  ( 140 )
131 Analysis of effects on revolution of humanity-education teaching contents in nursing department
MAO Chun1 KONG Lingyu2▲ HUANG Chunrong2 LI Yanhui2 SU Xuehua2 WEI Xiaoli2
Objective To analyze the real effects on humanity-education teaching contents reform in Nursing Department. Methods By reforming the humanity-Edu-1st-class contents as well as developing effective course resources of clinical medicine humanity. 76 year-2 nursing students in Hubei University of Arts and Science were randomly divided into experimental group （39 students） and control group （37 students）. Contents of experimental group were nurse′s humanity and medicine humanity; control group was offered common teaching contents （nurse′s humanity）. The sets of class, students′ before-and-after and total behavior assessments were compared. Results The survey results of the set of first class course of humanistic quality education in the two groups showed that, satisfaction rate of each survey dimension of the experimental group was higher than those of the control group, the differences were statistically significant (P < 0.05); the scores of each dimension of humanistic quality before and after the intervention of the two groups were compared, the scores of moral quality, cultural quality, legal quality and psychological quality of the experimental group was superior to the control group (P < 0.05); there was no statistically significant difference in aesthetic quality (P > 0.05). Conclusion Reforming teaching contents, developing course resources of clinical medicine humanity to enrich the 1st-class, can improve quality of student humanity, the teaching effect is obvious, worthy of the peer reference and promotion.
2018 Vol. 15 (1): 131-135 [Abstract] ( 252 ) HTML (1 KB)  PDF (653 KB)  ( 115 )
136 Application research of "flip classroom" teaching mode based on video micro-course in nursing teaching of spine surgery
CHANG Chunjia YU Mei ZHANG Yiying
Objective To explore the application effect of "flip classroom" teaching mode based on video micro-course in nursing teaching of spine surgery. Methods According to the student ID of nursing students, 126 directed interns of Tianjin Medical College in Department of Spinal Surgery, the 309th Hospital of Chinese People's Liberation Army from June 2014 to June 2015 were divided into the control group and observation group randomly, with 63 interns in each group. The control group was taken traditional teaching methods for teaching management, while the observation group utilized "flip classroom" teaching mode based on video micro-course on the basis of traditional teaching methods. After 2 weeks of internship, the theory scores, clinical operation scores and adverse events rates of the two groups were compared. Results After 2 weeks of internship, more than 95% of the nursing students in the observation group were agreed with the "flip classroom" teaching mode based on the video micro-course. Besides, both of the theory and the clinical operation scores of the observation group were higher than those of control group, the rate of adverse events in the observation group was lower than that of the control group, the differences between the two groups were all statistically significant (P ＜ 0.05). Conclusion It is possible to increase the enthusiasm of nursing students to enter clinical practice and improve the clinical operation skills and the relationship between teachers and students by using the teaching method of "flip classroom" teaching mode based on video micro-course. Therefore, it is also possible to reduce the incidence rates of nursing disputes and improve the satisfaction of nursing students in clinical teaching.
2018 Vol. 15 (1): 136-138 [Abstract] ( 252 ) HTML (1 KB)  PDF (635 KB)  ( 109 )
139 Discussion on new teaching methods of medicinal chemistry
WEI Zhao LI Xiaoye JIANG Ru YAO Lin LIU Xueying
The strategies to improve the teaching method of medicinal chemistry according to its characteristics was put forward in this paper, it was advocated that the teaching should take the students as the main body, and use the heuristic teaching mode to stimulate the students take the initiative to think. Teachers should explore the teaching resources from daily life and link the textbook closely with practice to highlight the practical value of medicinal chemistry, increasing interest in learning. Meanwhile, special attention should be paid to experimental teaching to cultivate students′ practical ability and scientific innovation ability, laying a good foundation for the cultivation of innovative and applied pharmaceutical talents.
2018 Vol. 15 (1): 139-141 [Abstract] ( 302 ) HTML (1 KB)  PDF (627 KB)  ( 99 )
142 Exploration on the pharmacology teaching practice of cultivation of outstanding innovative ability and medical humanistic quality
YAO Jihong LYU Li GAO Dongyan ZHOU Junjun
The cultivation of outstanding innovative ability and medical humanistic quality is very important for cultivating high-quality medical talented persons. In the teaching practice of pharmacology, we focus on integrating the improvement of innovative ability and humanistic quality into the teaching process. The students not only learn knowledge, but also improve various abilities especially the innovative ability of clinical thinking. The teaching practice is conducive to the cultivation of medical talented persons with exquisite technology, noble medical ethics.
2018 Vol. 15 (1): 142-144 [Abstract] ( 306 ) HTML (1 KB)  PDF (594 KB)  ( 118 )
145 Application of teachers standardized patients in the teaching of gynecology and obstetrics
ZHANG Yuanyuan ZHU Mingyue GAO Dongmei▲ Guzalinur·Ashley
Objective To explore the application value of teachers standardized patients (TSP) in clinical teaching practice of obstetrics and gynecology. Methods A total of 115 students from the five-year clinical medicine specialty of 2013 in the Third Clinical Medical College of Xinjiang Medical University were selected as research objects and randomly divided into two groups: experimental group (60 students) and control group (55 students). They were taken TSP teaching and regular teaching respectively. At the end of the teaching, the theoretical and clinical skills in the two groups were assessed; at the same time, the teaching satisfaction of the two groups for the teaching situation was surveyed. Results The satisfactory rate of the students in the experimental group was significantly higher than that in the control group, the theoretical scores of the students in the experimental group were significantly higher than those in the control group, the differences were all statistically significant (P ＜ 0.05). There was no statistically significant difference of the scores of writing complete medical records between the two groups (P ＞ 0.05). The scores of history-taking, gynecologic examination, diagnostic basis, differential diagnosis, doctor-patient communication in the students of experimental group were higher than those of the control group(P ＜ 0.05). While there was no statistically significant difference of the scores of making plans of diagnosis and treatment and the accept attitude between the two groups (P ＞ 0.05). Conclusion In the clinical teaching of obstetrics and gynecology, the application of TSP can improve the clinical practice skills of medical students.
2018 Vol. 15 (1): 145-149 [Abstract] ( 307 ) HTML (1 KB)  PDF (620 KB)  ( 133 )
150 A study on the relative weight setting of diagnosis-related groups by three methods
PENG Weibin1,2 LIUYan1▲ MEI Jing1
Objective To explore the relative weight setting of diagnosis related groups. Methods Based on the five categories of cost composition of the cost of hospitalization, through three methods to determine the proportion of various types of costs, and calculated the each DRG group relative weight on Peking University Shenzhen Hospital through the CN-DRGs (2016 version). Results The number of cases admitted to the study in the hospital in 2016 was 57719. The DRG group distribution, total weight, CMI was not very different among the three methods of calculation results. The total weight and CMI was respectively 57523.54, 57538.42, 56955.98 and 0.9966, 0.9969, 0.9868. Conclusion To apply DRGs for the performance evaluation of capacity dimension, the relative weight of each DRG group should be further studied in combination with clinical experience and statistical method.
2018 Vol. 15 (1): 150-154 [Abstract] ( 289 ) HTML (1 KB)  PDF (380 KB)  ( 68 )
155 The effect of postoperative system nursing intervention on the physical function of children after the orthopaedic surgery for developmental dislocation of the hip
WANG Xiaolei HU Xiaoyun LI Lianyong
Objective To explore the effect of systemic nursing intervention on the physical function of children after the orthopaedic surgery for developmental dislocation of the hip. Methods Ninety cases of children with developmental dislocation of the hip accepted orthopaedic surgery and treated in Shengjing Hospital of China Medical University from August 2014 to August 2016 were selected, according to the random number tables children were divided into control group and observation group, 45 cases in each group. The control group was given conventional nursing, the observation group was given the systematic nursing intervention, both groups were given continuous nursing for 2 months. The nursing satisfaction of family members, complications of children, lower limb motor function of children in 6 months of followed up, the quality of life, and Harris hip score were compared between two groups. Results The nursing satisfaction of the family members in the control group was 75.56%, and the nursing satisfaction of the family members in the observation group was 93.33%, the satisfaction degree of the family members in the observation group was higher than that in the control group, the difference was statistically significant (P < 0.05). There was no significant difference in the incidence of common complications in the two groups (P > 0.05). Compared with before treatment, the scores of motor function of the lower extremities, the quality of life, and the Harris score of hip joints in the two groups were increased after treatment, and the above scores of the observation group after treatment were higher than those of the control group, the differences were statistically significant (P < 0.05). Conclusion For the children with developmental dislocation of the hip, postoperative nursing intervention can significantly improve the nursing satisfaction of the family members, reduce the incidence of postoperative complications, and effectively promote the recovery of limb function.
2018 Vol. 15 (1): 155-158 [Abstract] ( 239 ) HTML (1 KB)  PDF (553 KB)  ( 134 )
159 The influence of recurrent respiratory tract infection children and patriarch by continuous nursing intervention
CHEN Lixin1 XU Hao2
Objective To approach the influence of continuous nursing intervention on recurrent respiratory tract infection children and patriarch. Methods The 76 cases clinical data of recurrent respiratory tract infection children in Affiliated Central Hospital of Shenyang Medical College from January 2012 to January 2013 were analyzed，which was to be divided into two groups by different nursing intervention, control group (38 cases) and observation group (38 cases). The disappearance time of clinical symptoms, disease knowledge score, treatment compliance of children and the children patriarch satisfaction of the two groups were detected. Results The fever disappearance time, cough disappearance time, occlusion disappearance time, pharyngeal tonsil congestion and disappearance time of observation group  children were lower than control group, the children treatment compliance and children patriarch satisfaction of observation group were higher than those of the control group, the life rule, environmental sanitation, conventional treatment, fitness exercise, acute episode management of the observation group  children patriarch were higher than control group, the differences were statistical significance (P < 0.05). Conclusion The application of continuous nursing intervention in children with recurrent respiratory tract infection can improve clinical symptoms，increase disease knowledge level of children′s patriarch, treatment compliance of children and the children′s patriarch satisfaction，it is worthy of clinical application.
2018 Vol. 15 (1): 159-162 [Abstract] ( 267 ) HTML (1 KB)  PDF (614 KB)  ( 152 )
163 Effect of comprehensive nursing intervention on the curative effect and psychological factors of deciduous tooth pulp disease and periapical disease
FENG Hongjing1 QIAN Ping′an2 CHEN Wenying1 WANG Yong1
Objective To investigate the effect of comprehensive nursing on the curative effect and psychological factors of deciduous tooth pulp disease and periapical disease. Methods The 154 cases of deciduous tooth pulp disease or periapical disease were treated in the Stomatological Center of Affiliated Hospital of Medical College of Ningbo University from April 2014 to December 2016, they were divided into intervention group (77 cases) and routine nursing group (77 cases) according to the random number method. The children in the routine group were given routine nursing support, while the comprehensive group was given comprehensive nursing intervention. The treatment effect, postoperative pain rate and pain degree, compliance in treatment and nursing service satisfaction of the two groups were compared. Results The total effective rate of the children in the comprehensive care group was significantly higher than that in the routine nursing group (P < 0.05); and the indexes of gingival index, depth of diagnosis, plaque index and bleeding index of gingival bleeding in children with comprehensive nursing intervention were significantly better than those in routine care group (P < 0.05); the incidence of postoperative pain and pain score of the comprehensive care group were lower than those of the routine care group, the differences were statistically significant (P < 0.05); the children′s compliance and the family nursing satisfaction of the comprehensive care group were higher than those of the routine care group, the differences were statistically significant (P < 0.05). Conclusion Comprehensive nursing intervention can significantly improve the treatment effect of deciduous tooth pulp disease and periapical disease, improve the oral condition of children, reduce the incidence of pain and the degree of pain, and can effectively improve children′s compliance and family nursing satisfaction, worthy of clinical promotion.
2018 Vol. 15 (1): 163-166 [Abstract] ( 227 ) HTML (1 KB)  PDF (602 KB)  ( 142 )
167 The effects of psycho-care intervention on the negative emotional and compliance for the young ischemic stroke patients
CHENG Min1 WANG Xinyan2 ZHANG Cheng2
Objective To explore the effect of psychological nursing intervention on the negative emotional and compliance for patients with ischemic stroke. Methods From April 2016 to April 2017, 90 cases of young patients with ischemic stroke were treated in Wanbei Coal and Electricity Group General Hospital were selected and divided into the control group and the observation group according to the random number table method, 45 cases in each group. The control group was given routine nursing care, the observation group on based of the control group was given psychological nursing intervention. Both groups were continuously nursing for 3 months. The nerve function defect score (NIHSS) scores, daily life ability score (ADL) before and after treatment, and the negative emotions and compliance were compared between two groups. Results After nursing, the NHISS scores decreased significantly and the ADL scores of two groups increased significantly compared with before the nursing. The scores of NHISS and ADL in the observation group were significantly improved compared with those in the control group, the differences were statistically significant （P ＜ 0.05）. After nursing, the scores of SAS and SDS in the two groups decreased significantly compared with those before nursing, and the scores of SAS and SDS in the observation group were significantly lower than those in the control group, the differences were statistically significant （P ＜ 0.05）. The compliance rate of the control group was 75.56% after treatment, and the compliance rate of the observation group was 93.33% after treatment, there was a significant difference in the treatment compliance between the observation group and the control group, the difference was statistically significant （P ＜ 0.05）. Conclusion In the process of treatment young patients with ischemic stroke, psychological nursing intervention on base of routine nursing can help promote recovery of neurological function and daily living ability, relieve negative emotions and improve treatment compliance.
2018 Vol. 15 (1): 167-170 [Abstract] ( 296 ) HTML (1 KB)  PDF (553 KB)  ( 113 )
171 A systematic review of the effect of comprehensive nursing intervention on pulmonary tuberculosis patients in domestic in mentation
SHEN Yanqin
Objective To evaluate the effect of comprehensive nursing intervention on pulmonary tuberculosis patients in domestic in mentation. Methods Databases including CNKI, CBM, VIP and WanFang Data were searched to collect randomized controlled trials (RCTs) related with comprehensive nursing intervention on pulmonary tuberculosis patients in mentation from Janurary in 2011 to April in 2017. One researcher screened literature independently, extracted data and evaluated the risk of bias of included studies. Meta-analysis was performed via RevMan 5.3 software. Results A total of 13 RCTs were included, which involved 1790 patients. The results of meta-analysis showed that the decrease of SAS scores in comprehensive nursing intervention group of the middle-youth-age patients, the middle-age patients, the quinquagenarian patients and the gerontism patients were all significantly higher than that in usual nursing group [MD=6.80, 95%CI (5.30, 8.30), P ＜ 0.000 01; MD=9.35, 95%CI (7.06, 11.65), P ＜ 0.000 01; MD=9.09, 95%CI (5.54, 12.65), P ＜ 0.000 01; MD=28.05, 95%CI (24.13, 31.97), P ＜ 0.000 01]. The decrease of SDS scores in comprehensive nursing intervention group of the middle-youth-age patients, the middle-age patients, the quinquagenarian patients and the gerontism patients were significantly higher than that in usual nursing group [MD=9.05, 95%CI (5.52, 12.58), P ＜ 0.000 01; MD=9.47, 95%CI (8.11, 10.82), P ＜ 0.000 01; MD=7.48, 95%CI (3.24, 11.11), P=0.0004; MD=23.10, 95%CI (22.46, 23.74), P ＜ 0.000 01]. Conclusion Comprehensive nursing intervention could effectively improve mental state of patients with pulmonary tuberculosis.
2018 Vol. 15 (1): 171-176 [Abstract] ( 285 ) HTML (1 KB)  PDF (1484 KB)  ( 123 )
177 Effect of quality nursing based on clinical pathways on the incidence of rehabilitation and complication after coronary artery stenting in patients with coronary heart disease
YANG Lijun MA Hongbo LI Juan ZHANG Fenlan
Objective To explore the effect of quality nursing based on clinical pathways on the incidence of rehabilitation and complication after coronary artery stenting in patients with coronary heart disease. Methods From April 2014 to June 2016, 62 patients with coronary heart disease in the department of cardiology of Guangyuan Central Hospital were randomly divided into control group and study group, 31 cases in each group. The control group was treated by routine nursing, and the study group carried out quality nursing based on clinical path on the basis of routine nursing. The incidence of complications, nursing satisfaction, left ventricular ejection fraction (LVEF), depression score (SDS) and anxiety score (SAS) at the time of admission and after care were compared. Results After treatment, the LVEF of the two groups increased significantly than before care, and the study group was higher than the control group, the difference was statistically significant (P < 0.05). After treatment, the SDS and SAS scores of the two groups were lower than those before treatment, and the study group was lower than the control group, the difference was statistically significant (P <0.05). The rate of radial spasm, the hematoma, the vagal reflex and the arrhythmia in the study group was lower than that in the control group, the difference was statistically significant (P < 0.05). The overall satisfaction of the study group was higher than that of the control group, the difference was statistically significant (P < 0.05). Conclusion Coronary heart disease patients underwent coronary stent implantation based on the clinical path of the quality of care is significant, it can effectively promote the recovery of cardiac function after surgery to relieve its negative emotions, reduce the incidence of complications and improve nursing satisfaction, it is worth promoting
2018 Vol. 15 (1): 177-180 [Abstract] ( 270 ) HTML (1 KB)  PDF (597 KB)  ( 128 )
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