淫羊藿苷对微重力下骨髓间充质干细胞增殖与凋亡的影响

Abstract
Figure/Table
References (20)
Related Citation (15)

Download: PDF (1230 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the effect of icariin (ICA) on the proliferation and apoptosis of bone mesenchymal stem cell (BMSCs) under microgravity. Methods The BMSCs were randomly divided into three groups: microgravity group, dosing group and control group. The microgravity group and dosing group was treated with microgravity, besides, 1×10-6 mol/L ICA was applied to the cells in dosing group; the control group was cultured without any additional operation. Cell proliferation was detected by MTT assay, each group was set up 3 reduplicateholes; the apoptosis was detected by FCM, each group was detected 3 times; the expression of apoptosis related genes were detected by QPCR with 3 reduplicate holes, and the subcellular structure was observed by TEM. Results The result of MTT examination showed that the absorbance value of microgravity group was significantly lower than that of the control group (P < 0.01), and the dosing group was significantly higher than that of the control group (P < 0.01). FCM method showed that the apoptosis rate of microgravity group was significantly higher than control group (P < 0.01), and the apoptosis rate of the dosing group was significantly lower than control group (P < 0.01). Compared with the control group, the expression of Bcl-2, Bax/bcl-2, Bax, and P53 gene was up-regulated in microgravity group (all P < 0.05); in dosing group, the expression of Bax, Bcl-2, P53 and the Bax/bcl-2 gene was significantly down-regulated when compared with microgravity group (all P < 0.01). TEM indicated that the dosing group cells were generally normal and with no obvious changes when compared with the control group; in microgravity group, the cells existed membrane shrinking, microvilli decreasing, cytoplasmic concentration, ribosome and mitochondria gathering, nucleus pyknosis, and dense chromatin gathering to the margin of nuclear membrane. Conclusion Microgravity can inhibit osteoblast′s proliferation and promote apoptosis of BMSCs. But the appropriate concentration of ICA can protect BMSCs from the harmful effect caused by microgravity.

Key words： Bone mesenchymal stem cell Icariin Microgravity Proliferation Apoptosis

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHANG Fang1* YAN Haiyang2* CHEN Xuyi2 LYU Dan3▲ LI Li2▲

Cite this article:

ZHANG Fang1* YAN Haiyang2* CHEN Xuyi2 LYU Dan3▲ LI Li2▲. Effects of icariin on proliferation and apoptosis of bone mesenchymal stem cell under microgravity[J]. 中国医药导报, 2018, 15(1): 10-13,21.

URL:

http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I1/10

[1] 郭中平.2015新版中华人民共和国药典与中国生物医药产业发展[J].中国新药杂志，2015，24（20）：2352-2356.
[2] 赵文静，王历，王芝兰，等.淫羊藿的药理作用及临床应用研究进展[J].中医药信息，2016，33（2）：105-108.
[3] Chen KM，Ge BF，Liu XY，et al. Icariin inhibits the osteoclast formation induced by RANKL and macrophage-colony stimulating factor in mouse bone marrow culture [J]. Pharmazie，2007，62（5）：388-391.
[4] 朱鸿飞，郑军，徐小燕，等.不同浓度淫羊藿苷修复骨损伤：争议与探索[J].中国组织工程研究，2014，18（2）：301-306.
[5] 牟欢，刘洋，李俊杰，等.骨髓间充质干细胞在重度烧伤大鼠心肌损伤中的保护作用[J].中国医药导报，2017，14（12）：12-16.
[6] Korn C，Méndez-Ferrer S. Myeloid malignancies and the microenvironment [J]. Blood，2017，129（7）：811-822.
[7] 李军，张春秋，宋光明，等.高重力下成骨细胞的力学生物学响应[J].医用生物力学，2016，31（3）：278-283，290.
[8] 孟芮，续惠云，王哲，等.模拟失重骨细胞条件培养基对成骨细胞活性的影响[J].航天医学与医学工程，2009，22（4）：247-251.
[9] 李静，陈军，李秀玉，等.模拟微重力对骨髓间充质干细胞全细胞瘤苗干预A549移植瘤增殖的研究[J].中国医药导报，2016，13（3）：8-12.
[10] 毛新建.模拟微重力对骨髓间充质干细胞增殖和迁移的影响及相关分子机理研究[D].重庆:重庆大学，2014.
[11] 黄懿文.模拟微重力状态下过氧化物酶体增殖物激活受体γ基因表达对成骨分化的影响[D].广州：南方医科大学， 2013.
[12] 李钢.淫羊藿苷及富自体浓缩生长因子促进兔颅骨缺损修复的分子机制探讨[D].石家庄：河北医科大学2015.
[13] 黄国平，郑强，杨金凤，等.模拟微重力对人骨髓间充质干细胞向成骨细胞分化中细胞信号通路影响的分析[J].空间科学学报，2008（1）：87-96.
[14] 石东燕.模拟微重力对人骨髓间充质干细胞向成骨细胞分化的影响[D].杭州：浙江大学，2010.
[15] 刘天麟，张学斌，张忻，等.淫羊藿苷对犬骨髓间充质干细胞增殖、成骨和成脂分化的影响[J].口腔颌面外科杂志，2014，24（4）：276-280.
[16] Balaburski GM，Hontz RD，Murphy ME. p53 and ARF：unexpected players in autophagy [J]. Trends Cell Biol，2010， 20（6）：363-369.
[17] 胡汪来，吴缅.p53磷酸化修饰及其功能研究进展[J].中国科学：生命科学，2015，45（11）：1093-1100.
[18] 杜联芳，刘望彭.诊断超声辐照后大鼠睾丸生精细胞P53 mRNA、Bcl-2 mRNA表达改变[J].中华超声影像学杂志，2001，（3）：53-55.
[19] 李晓宁，迟蕾，吴磊，等.头针对急性脑梗死大鼠不同时间窗Bax与Bcl-2影响的研究[J].针灸临床杂志，2016， 32（4）：73-76，97.
[20] Salakou S，Kardamakis D，Tsamandas AC，et al. Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis [J]. In Vivo，2007，21（1）：123-132.