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2017 Vol. 14, No. 30
Published: 2017-10-25
 
 
4 Establishment and verification of TaqMan-MGB two-color fluorescent probe method for detecting the gene polymorphisms of insulin receptor substrate 1 rs1801278
ZHAO Yangyang1,2 XU Zhihui2 LIU Yan2 WU Rongrong3 LIU Xinguang1 XU Dongping2▲ HAN Jin3▲
Objective To establish an experimental method suitable for clinical routine detection of the gene polymorphisms of insulin receptor substrate 1 (IRS1) rs1801278. Methods The peripheral blood samples of 200 healthy controls in 302 Military Hospital of China from March 2015 to June 2016 were collected, the specific primers and fluorescent probe aimed at the polymorphic sites of gene polymorphisms of IRS1 rs1801278 were designed, and the gene polymorphisms of rs1801278 were detected by the TaqMan-MGB real-time fluorescence polymerase chain reaction and validated by gene sequencing method (golden standard). The distribution frequencies of different genotypes of IRS1 rs1801278 in this population were discussed as well. Results The TaqMan-MGB two-color fluorescent probe method could identify 3 genotypes of IRS1 rs1801278, achieving 100% consistence with gene sequencing method. The distribution frequencies of T/T, C/T and C/C genotypes of gene polymorphisms of IRS1 rs1801278 in this population were 0%, 1% and 99%, respectively. Conclusion The established TaqMan-MGB fluorescent probe method based on the specific probe and primer of single nucleotide polymorphisms is rapid and effective in identifying the genotypes of gene polymorphisms of IRS1 rs1801278, the results are reliable, and the operation is easy, but the predictive value for the individualized therapeutic effect of Chinese Han type 2 diabetes patients is limited, which is not recommended for clinical routine practice.
2017 Vol. 14 (30): 4-8 [Abstract] ( 508 ) HTML (1 KB)  PDF (1438 KB)  ( 285 )
9 The influence of Abnormal Savda Munziq on mRNA expressions of Id4 and p21 genes in abnormal savda hepatocellular carcinoma rat models
Zulipikaer·Abudureheman WANG Yanjiao Nazilamu·Yusufujiang Gulinigeer·Xuehelati Sikandeer·Baikeli
Objective To investigate the anticancer role of Uyghur medicine Abnormal Savda Munziq (ASM) in abnormal savda hepatocellular carcinoma rat models. Methods 90 male Wistar rats were divided into 6 groups, including normal group, control hepatic carcinoma group, abnormal savda hepatocellular carcinoma group and ASM high, middle and low dosage group by random number table, with 15 cases in each group. The normal group rats were placed in normative feeding environment. After 21 days of feeding in laboratory environment, the control hepatic carcinoma group rats were induced by diethylnitrosamine (DEN) 20 weeks to establish the liver cancer rat model. The rats in the abnormal savda hepatocellular carcinoma group, ASM high, middle and low dosage group were established abnormal black bile models, through dry cold property feed, dry cold climate environment, plantar electrical stimulation, tail and forced swimming, etc; after 21 days they were induced by DEN for 20 weeks to establish the abnormal savda hepatocellular carcinoma models. At the same time, the rats in the ASM high, middle and low dosage group were intervened by 6.0, 3.0, 1.5 g/kg of ASM. The mRNA was extracted from liver tissue and Id4, p21 gene were verified by RT-qPCR after screened by gene chip technology. Results The Id4 gene expression in liver tissue was down-regulated in control hepatic carcinoma group ,compared with the normal control group (P ＜ 0.01). The Id4 gene expression in liver tissue was down-regulated in abnormal savda syndromeliver cancer group, compared with the control hepatic carcinoma group (P ＜ 0.01). Compared with the abnormal savda hepatocellular carcinoma group, the expression of Id4 gene in liver tissue was up-regulated all in ASM high, middle and low dosage group, the high and middle dosage groups were obviously up-regulated (P ＜ 0.01). Compared with the normal group, the liver tissue p21 gene expression was up-regulated in control hepatic carcinoma group (P ＜ 0.01). The p21 gene expression in liver tissue wasup-regulated in abnormal savda syndromeliver cancer group, compared with the control hepatic carcinoma group (P ＜ 0.05). Compared with the abnormal savda hepatocellular carcinoma group, the expressions of p21 gene were down-regulated all in ASM high, middle and low dosage group, the middle and low dosages group were dramatic decline (P ＜ 0.01). Conclusion The ASM could have an impact on mRNA expressions of Id4 and p21 gene in abnormal savda hepatocellular carcinoma rat models.
2017 Vol. 14 (30): 9-12,17 [Abstract] ( 230 ) HTML (1 KB)  PDF (736 KB)  ( 74 )
13 Protective effect and mechanism of ginseng extract on human neuroblastoma injury induced by hydrogen peroxide
ZHANG Huiyuan YANG Qing SUN Jiaming LIU Yingna LIN Jia′nan SHI Xiaozheng LI Na QU Xiaobo
Objective To observe the influence of ginseng extract on the injury induced by hydrogen peroxide in human neuroblastoma cells (SH-SY5Y) and explore its mechanism. Methods The optimal concentrations of panaxadiol saponins (PDS), ginsenoside(GS) and ginseng non-saponin(GN) were determined by the MTT assay. SY5Y cells in the logarithmic growth phase were supplemented with hydrogen peroxide (final concentration of 300 μmol/L) and were incubated for 4 h. The cell injury model was established. SY5Y cells were divided into model control group, PDS group, GS group and GN group. A blank control group was added to an equal volume of cell culture fluid. MTT assay was used to detect the cell viability. The protein expressions of Nrf2, Keap1, Bcl-2, Bax, Caspase3 and Caspase9 were detected by Western blot. Results Compared with the blank control group, the survival rate of SH-SY5Y cells in the model control group was significantly decreased (P < 0.01). Compared with the model control group, the survival rates of PDS group, GS group and GN group increased, and the concentration of 1, 10, 240 μg/mL was selected as test concentration in PDS group, GS group and GN group (P < 0.01). Compared with the blank control group, the protein levels of Keap1, Bax, Caspase3 and Caspase9 in SH-SY5Y of model control group were significantly increased (P < 0.05 or P < 0.01), and the protein levels of Bcl-2 and Nrf-2 were significantly decreased (P < 0.01). Compared with the model control group, in PDS group and GS group the protein levels of Keap1, Bax, Caspase3 and Caspase9 were significantly decreased (P < 0.05 or P < 0.01), and the protein levels of Bcl-2 and Nrf-2 were significantly increased (P < 0.01). Conclusion PDS and GS from ginseng extract can protect human SH-SY5Y cells from the hydrogen peroxide-induced damage, and the mechanism may inhibit the oxidative stress by activating the Nrf-2 pathway in SH-SY5Y cells, and reduce apoptosis.
2017 Vol. 14 (30): 13-17 [Abstract] ( 237 ) HTML (1 KB)  PDF (913 KB)  ( 177 )
18 Influence of glucose carbon source on the constitutive expression of recombinant human C-reactive protein
LI Junming YANG Liping WANG Chenyu ZHAO Qi ZHENG Qing SUN Chengming▲
Objective To investigate the effect of different concentrations of glucose on the constitutive expression of recombinant human C-reactive protein (rhCRP) in Pichia pastoris, and to screen out the optimum concentration of the glucose carbon source. Methods The different concentrations [0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% and 3.5% (w/v)] of glucose were used to constitutively express rhCRP in Pichia pastoris. Samples were taken from individual tubes of the cultures at the desired time points and the expression of rhCRP in the culture supernatant was analyzed by Western blot and gray scale. Results When rhCRP was constitutively expressed in Pichia pastoris X-33 using glucose as carbon source, 1.0% glucose (w/v) was selected as the best carbon source. Conclusion The concentration of glucose carbon source plays an important role in the constitutive expression of rhCRP in Pichia pastoris. The results of this study will provide a strong experimental basis for further optimized expression and large-scale production of rhCRP.
2017 Vol. 14 (30): 18-20,41 [Abstract] ( 276 ) HTML (1 KB)  PDF (1218 KB)  ( 4651 )
21 Role of UNR in regulating the epithelial mesenchymal transition of epithelial ovarian cancer cells
WENG Jianrong MIAO Yi SUN Yunyan
Objective To detect the expression of UNR gene in epithelial ovarian cancer cells, and to observe the effect of UNR gene on epithelial mesenchymal transition (EMT) of ovarian cancer cells. Methods Real-time quantitative PCR (qPCR) was used to detect and compare the expression of UNR gene in human epithelial ovarian cancer cell lines SKOV-3, HO-8910, ES-2 and immortalized human ovarian epithelial cell lines MOODY in vitro. qPCR and Western blot were used to detect the UNR-RNAi interfering efficiency of ovarian cancer cell SKOV-3, the invasion ability of SKOV-3 cells was detected by Transwell invasion assay. The protein expression of E-cadherin in SKOV-3 cells was detected by Western blot. Results UNR was significantly expressed in the epithelial ovarian cancer cell lines SKOV-3, HO-8910 and ES-2. After SKOV-3 was transfected by shUNR, Transwell assay showed that the number of transmembrane cells in UNR-RNAi group was (29.33±5.14) per field, which was significantly lower than that in negative control group [(76.00±5.42) per field] (P ＜ 0.01). In addition, Western blot showed that the expression of E-cadherin protein in SKOV-3 cells was significantly up-regulated after UNR gene interference. Conclusion shUNR can markedly inhibit the expression of UNR gene in ovarian cancer SKOV-3 cells, decrease the invasive ability of ovarian cancer cells. The up-regulated expression of E-cadherin after interference shows that UNR may promote the occurrence of ovarian cancer EMT, thus, the interfering technology taking UNR gene as target is expected to become a new strategy for the treatment of ovarian cancer.
2017 Vol. 14 (30): 21-24,69 [Abstract] ( 273 ) HTML (1 KB)  PDF (1265 KB)  ( 150 )
25 Clinical effect of Wusheng Tincture combined with WHO "three stage" analgesic regimen in treating patients with mild and moderate cancer pain
HE Wenfeng1 YANG Xiaobing1 XU Lei2 ZHANG Liling3 LI Qiuping1 BI Jiayi1 LI Qiuxia4 DENG Hong1
Objective To observe the clinical effect of traditional Chinese medicine Wusheng Tincture combined with WHO "three stage" analgesic regimen in the treatment of mild and moderate cancer pain. Methods One hundred and twenty-six patients with cancer pain treated in Department of Oncology, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine from September 2015 to February 2017 were selected as research objects, and they were randomly divided into the treatment group (65 cases) and control group (61 cases). According to numerical rating scale, in the treatment group, mild pain patients accounted for 50.8% (33/65), moderate pain patients accounted for 49.2% (32/65), while in the control group, mild pain patients accounted for 50.8% (31/61), moderate pain patients accounted for 49.2% (30/61). The control group was taken WHO "three stage" analgesic regimen and the pain site was treated with placebo tincture; the treatment group was treated with Wusheng Tincture on the pain site on basis of control group. The two groups were treated for 7 days as one course. NRS was used to assess the pain level of the patients, the brief pain inventory (BPI) was used to evaluate the quality of life, the clinical effects and adverse reactions between the two groups were observed. The data was analyzed and processed by SPSS 18.0. Results A total of 120 patients completed the study (6 patients were excluded for the external use of cold stimulation). After treatment, the NRS scores in the two groups were all lower than those before treatment (P ＜ 0.01), and the NRS score of the treatment group was significantly lower than that of the control group, the difference was highly statistically significant (P ＜ 0.01). After treatment, the scores of BPI in the two groups were all significantly lower than those before treatment (P ＜ 0.05), and the BPI score of the treatment group was significantly lower than that of the control group, the difference was highly statistically significant (P ＜ 0.01). The BPI score of patients with mild pain in the treatment group after treatment was significantly lower than that of control group (P ＜ 0.05), while there was no significant difference of the BPI score between the patients with moderate pain in treatment group and control group (P ＞ 0.05). There was no statistically significant difference of total effective rate between the two groups (P ＞ 0.05). The incidence of constipation with Ⅳ degree in the treatment group was significantly lower than that in the control group (P ＜ 0.01), and the overall incidence of adverse reactions in the treatment group was significantly lower than that in the control group (P ＜ 0.05). Conclusion Compared with patients with moderate pain, external use of Wusheng Tincture has better effects for the analgesic efficacy and improvement of quality of life.
2017 Vol. 14 (30): 25-29 [Abstract] ( 310 ) HTML (1 KB)  PDF (706 KB)  ( 218 )
30 Analyzing gene expression profiles in ventricular remodeling after myocardial infarction via bioinformatics methods
NIU Xiaowei1,2 ZHANG Jingjing3 PENG Yu2,4 BAI Ming2,4 ZHANG Zheng2,4
Objective To identify the molecular mechanisms underlying ventricular remodeling after myocardial infarction using bioinformatics methods. Methods Microarray data about ventricular remodeling after myocardial infarction were searched from GEO database. Results The array data of GSE47495 were obtained. A total of 611 DEG were identified. Gene ontology analysis showed that the DEG significantly enriched in biological processes, molecular function, and cell component. Pathway analysis showed the DEGs were involved in signaling pathways. The top 3 hub genes, Acox1, Anxa1, and F13a1 were identified from protein-protein interaction network. Results from drug screening indicated that palm oil and curcumin may regulate the above genes. Conclusion During post infarction ventricular remodeling, the identified DEG are mainly enriched in metabolism, extracellular matrix, inflammation, and coagulation. The hub genes, Acox1, Anxa1, and F13a1 provid potential targets for treatment of ventricular remodeling. Palm oil and curcumin may improve the ventricular remodeling through the regulation of these genes.
2017 Vol. 14 (30): 30-33 [Abstract] ( 309 ) HTML (1 KB)  PDF (885 KB)  ( 399 )
37 The progress of GWAS in lung cancer research
YANG Xingli1 FU Yongquan1 LIU Xiaojun2 ZHANG Wei2 TAO Tao2
In the last decade, there have been numerous genome-wide association studies for lung cancer. The studies′ target population have expanded from Caucasians to multi-racial, incorporating the disease and genetic relations with environment factors, such as smoking, air pollution, gender and drug effects for deeper understandings. Through these studies, researchers discover series of genetic mutations related to different cancer types using high-density microarray and next generation sequencing technologies. Some of these mutation findings have high values in health management and clinical usages. This article systemic reviews current genome-wide association studies for lung cancer.
2017 Vol. 14 (30): 37-37 [Abstract] ( 244 ) HTML (1 KB)  PDF (503 KB)  ( 222 )
38 Research progress and application prospect of bone cement in osteoporotic vertebral compression fractures
CAO Weining1,2 GUO Tuanmao2 XING Yanli2 ZHU Haiyun2 LI Wanru1
With China gradually entering the aging society in recent years, the increasing osteoporotic vertebra compression fractures occurs in the elderly. After the conservative and open surgery, the treatment of OVCF has evolved into minimally invasive surgical procedures, which mainly include percutaneous vertebroplasty and percutaneous kyphoplasty. Obviously, they can facilitate a less trauma, pain relief, and low complication. Bone cement plays an important role in the two operations on account of both the recovery of vertebral height and subsequent pain relief and its great economic and social benefits. Therefore, this research on bone cement was introduced to guide the clinical practice in this paper.
2017 Vol. 14 (30): 38-41 [Abstract] ( 744 ) HTML (1 KB)  PDF (503 KB)  ( 184 )
42 Research progress of Sorafenib combined with locoregional therapy in advanced hepatocellular carcinoma
XIONG Lin XU Ximing
At present, the incidence and mortality rate of hepatocellular carcinoma (HCC) are on the rise. HCC is characterized by insidious onset, difficult early diagnosis, and rapid progression. Surgical treatment can only be used for less than 30% of patients, therefore non-surgical treatment used for prolonging the survival time of patients with advanced HCC is significant. As one of the non-surgical treatment of advanced HCC, Sorafenib, can only prolong overall survival time for several months. More than two thirds of patients with advanced HCC have died from liver failure due to the progression of intrahepatic tumors; local treatment can control the progress of intrahepatic tumors, but relapse is common. At present, a large number of clinical trials about combination Sorafenib with locoregional therapy have been carried out at home and abroad. This review, by describing the theory of combination therapy and the results of clinical trials, intends to reach a feasible conclusion that can be used as a reference for clinical doctors to make the treatment plan.
2017 Vol. 14 (30): 42-45 [Abstract] ( 269 ) HTML (1 KB)  PDF (506 KB)  ( 108 )
46 Research status of establishment of animal model of knee osteoarthritis
SONG Hanbing1 ZHAO Feng2 GE Yuanyuan2 YANG Dan2 YU Yingjun3▲
Based on the continuous exploration on the fundamental researches of etiology and treatment mechanism of knee osteoarthritis, this paper takes in-depth analysis and finds that the animals used for establishing animal models with osteoarthritis at present are mainly rats and rabbits, the application of other animals, such as dogs, pigs, chickens and others, is less. The commonly model replicating methods are spontaneous models, operation models, and other methods, different animals and different methods have relatively strict distinction, so the choice of models should be based on the need of study.
2017 Vol. 14 (30): 46-49 [Abstract] ( 275 ) HTML (1 KB)  PDF (502 KB)  ( 213 )
50 Investigation and analysis of physicians and pharmacists′ awareness and attitudes towards the implementation of the national essential medicine system in the secondary and tertiary hospitals in Nanjing City
SUN Xiaoluan1 ZHANG Yu2,3 ZHANG Kaichao1 ZHA Yueqi2 LI Xin1,2
Objective To evaluate the awareness and attitudes of physicians and pharmacists on the national essential drug system from secondary and tertiary hospitals in Nanjing, in order to provide policy suggestions for priority use of basic medications in the secondary and tertiary hospitals. Methods A self-designed questionnaire was used to investigate doctors and pharmacists from 15 hospitals in Nanjing City by representative sampling. Results About the selection of antibiotics and traditional Chinese medicines in essential drugs, pharmacists′ correct rates were respectively higher by 1.5 percent and 21.1 percent than physicians. The physicians′ and pharmacists′ average scores of familiarity for basic drug system were (15.73±3.62) points and (16.15±4.31) points (t=1.110, P=0.268). The physicians′ and pharmacists′ average scores of recognition were (17.31±3.35) points and (18.29±3.94) points (t=2.848, P=0.005). The proportions of physicians and pharmacists through professional training to understand the basic drug system were respectively 28.3% and 54.1% (χ2=31.497, P=0.000). Conclusion The pharmacists′ and physicians′ familiarity and recognition of the basic drug system are in the middle level, and pharmacists are higher than physician in recognition. There are differences in the understanding of the accessibility of essential drugs between physicians and pharmacists. The majority of physicians have not participated in related knowledge training or learning, and more than half of the pharmacist understand the basic drug system through professional training. 
2017 Vol. 14 (30): 50-54 [Abstract] ( 290 ) HTML (1 KB)  PDF (598 KB)  ( 75 )
55 Health education needs and influencing factors of patients with stable chronic obstructive pulmonary disease
LI Yang1 ZHONG Xiaohong2 GENG Renwen2
Objective To provide theory for better health education through investigating health education needs of patients with stable chronic obstructive pulmonary disease (COPD), and analyzing the factors as well. Methods A self-designed questionnaire was used to investigate 119 patients with stable COPD chosen by convenient sampling in three hospitals in Guangzhou from September 2016 to January 2017. Data were described by general statistics and analyzed by t-test, variance analysis or multiple linear regression analysis with SPSS 22.0 software. Results The score of disease awareness in COPD patients was (11.73±0.39) points, which showed that COPD patients were lack of disease cognition and the demand for health education was strong. Patients′educational level, occupation, income, medical insurance payment and duration of disease had influence on their health education needs (P < 0.05). Conclusion The demand for health education of patients with COPD is affected by many factors, so it is indispensable to fully assess their needs before carrying out health education of patients. Moreover, it′s suggested to improve patients′ health literacy so as to provide targeted and personalized health education.
2017 Vol. 14 (30): 55-58 [Abstract] ( 341 ) HTML (1 KB)  PDF (569 KB)  ( 89 )
59 Epidemiological survey of hand-foot-mouth disease in Zhangjiakou area from 2008 to 2012
WU Hongxia1 WANG Jie2 XU Xiaonan3 WANG Hao1
Objective To summarize the incidence of HFMD disease (HFMD) in Zhangjiakou area from 2008 to 2012, and explore its incidence trend in this area, in order to provide scientific basis for the future prevention and control strategy. Methods The data of HFMD disease came from the national disease surveillance information management system. The population data came from the census yearbook. Descriptive epidemiological analysis was adopted and Excel software was used to systemize the data. SPSS 16.0 software was used for statistical analysis. Results In Zhangjiakou area there were 20 595 cases suffered from HFMD disease from 2008 to 2012, and the male to female ratio was 1.38∶1, among whom children under 3 years old accounted for 49%, under 5 years old accounted for 81%. Scattered children accounted for 68.34%, nursery children accounted for 24.09%. In most areas, the onset time was mainly from May to October, and it has a single peak epidemic. The peak was from June to July. Since the beginning of 2008, the occurrence regularity presented a three-year cycle, and the morbidity increased sharply in the second year of each cycle, with the highest ratio in the region closing to Huailai County, Chicheng County around Beijing. Conclusion For the incidence trend of the HFMD in Zhangjiakou area, it′s needed to further strengthen the management of floating population, the prevention and treatment towards the scattered children under 5 years old is the major task, as well as pay more attention to the peak period of incidence from Jun to July, and the rule of the epidemic cycle.
2017 Vol. 14 (30): 59-62 [Abstract] ( 246 ) HTML (1 KB)  PDF (637 KB)  ( 108 )
63 Utilization and evaluation of community health service in the elderly under the mode of home-based care for the aged
MENG Haoting HU Lingjuan CUI Yuanyue
Objective To understand the status quo and evaluation of the use of community health services by the elderly in the city at present, to explore the improvement direction of community health institutions in terms of home care under existing conditions. Methods The method of convenience sampling was used. The self-made questionnaire was used to investigate 1433 elderly people in the country from July to December 2015. The data were analyzed by descriptive analysis and Logistic regression analysis. Results The community health service utilization rate was acceptable, 80% of the elderly had the experience of community health institutions treatment. The regression analysis showed that the elderly whose income was lower (P = 0.0009, OR = 2.1201), medical expenses accounted for a higher proportion of total income (P = 0.0181, OR = 0.5027) and who lived with children and their spouses (P = 0.0100, OR = 1.7564) were more inclined to choose community health services. The elderly aged 70-<80 or the elderly who was suffering from ≥ 3 kinds of chronic diseases had the highest utilization rate of community health services. The number of chronic diseases was the main influencing factor of the elderlys′ satisfaction with community health services (P < 0.05). Conclusion Community health institution plays a vital role in solving the nursing needs of home care for the elderly with its advantages of broad mass base, lower cost and close distance. Taking into account the different needs of medical care services with different living conditions and health status, community health institutions should pay more attention to the individual needs of the elderly.
2017 Vol. 14 (30): 63-69 [Abstract] ( 375 ) HTML (1 KB)  PDF (603 KB)  ( 78 )
70 Effect of dipeptidyl peptidase Ⅳ inhibitor on blood glucose fluctuation in patients with brittle diabetes mellitus by dynamic blood glucose monitoring system
LI Zhenghan XU Binhua▲ WANG Jing WANG Wei
Objective To observe the effect of dipeptidyl peptidase Ⅳ (DPP-4) inhibitor on blood glucose fluctuation in patients with brittle diabetes mellitus by dynamic blood glucose monitoring system. Methods 72 cases of outpatient and hospitalized patients with brittle diabetes from May 2013 to October 2016 in the First Hospital of Harbin City were selected, all patients enrolled after giving four intensive insulin (Insulin Aspart/Insulin Lispro + Glargine Insulin/Insulin Detemir) treatment, then continuously monitored the blood glucose of the patients with CGMS for 3 days. After 3 days, the patients were divided into DPP-4 group and control group by random number table method, each group of 36 cases. The above hypoglycemic scheme was continued to use in control group, Shah Glenn Dean 5 mg/d was used in DPP-4 group on the basis of control group. All patients were continuously monitored with CGMS before grouping, 1-3 days after grouping and 13-15 days after grouping. Blood glucose fluctuation [mean blood glucose fluctuation range (MAGE), standard deviation of blood glucose level (SDBG), maximum blood glucose fluctuation range (LAGE), mean blood glucose level (MBG), blood glucose> 10.0 mmol/L time percentage (PT10.0), blood glucose <3.9 mmol/L time percentage (PT3.9)], the amount of insulin and C peptide level between two groups were compared. Results Intra-group comparison: after grouping of 1-3 d, MAGE, MBG, LAGE, PT10.0 and PT3.9 of DPP-4 group were lower than before grouping (P < 0.05); after grouping of 13-15 d, the indicators of blood glucose fluctuations in DPP-4 group were lower than before grouping (P < 0.01); there were no significant differences on blood glucose fluctuation indexes before grouping and after grouping of 1-3 d in control group (P > 0.05); after grouping of 13-15 d, MBG, LAGE, PT10.0 in control group were lower than before grouping (P < 0.05). Comparison among groups: after grouping of 1-3 d, MAGE, LAGE, PT10.0 of DPP-4 group were lower than those of control group (P < 0.05); after grouping of 13-15 d, the indicators of blood glucose fluctuations in DPP-4 group were lower than those in control group (P < 0.05 or P < 0.01). The dosage of insulin after grouping of 15 d in DPP-4 group was significantly lower than before grouping (P < 0.01). There was a statistically significant difference on the amount of insulin between two groups after grouping of 15 d (P < 0.01). There was no significant difference on FC-P before and after grouping between two groups both intra-group comparison and comparison among groups (P > 0.05). Conclusion Shah Glenn Dean combined with insulin four times can improve blood glucose fluctuation in patients with fragile diabetes.
2017 Vol. 14 (30): 70-73,84 [Abstract] ( 268 ) HTML (1 KB)  PDF (759 KB)  ( 85 )
74 Effect of different dose of tranexamic acid local application on the postoperative drainage of total hip arthroplasty
HAO Shenshen LUAN Yanjun LIU Zhibin WANG Fei LIU Yanxiong LIU Jun
Objective To observe the effect of different dose of tranexamic acid (TXA) topical application on the postoperative drainage of total hip arthroplasty (THA). Methods 75 patients with primary unilateral THA from January to December 2016 were divided into three groups random number table, 25 patients in each group. The group A was treated by retrograde injection of 1.0 g (NS: 100 mL) TXA via the drainage tube when incision was sewed up. Group B and group C were injected with 2.0 g (NS: 100 mL) TXA and 3.0 g (NS: 100 mL) TXA in the same way, respectively. The main observation items were postoperative drainage volume, hemoglobin (Hb) of the third day after operation and number of blood transfusion. The secondary items included operation time, intraoperative blood loss, prothrombin time and activated partial thromboplastin time of the third day after surgery, number of pulmonary embolism, number of deep vein thrombosis and number of infections. Results There was no significant difference among the three groups in the base data (P > 0.05). In the main observation items, the postoperative drainage volume decreased successively; the group A was significantly larger than the group B and the group C respectively (P < 0.05), while the difference between the group B and the group C was not statistically significant (P > 0.05). The Hb of the third day after operation increased successively; group A was significantly lower than group B and group C respectively (P < 0.05), while there was no significant difference between group B and group C (P > 0.05). The incidence of blood transfusion was not statistically significant among the three groups (P > 0.05). There was no significant difference among the three groups in the secondary observation index (P > 0.05). Conclusion The topical application of 2.0 g TXA is superior to 1.0 g TXA in reducing the postoperative drainage volume of THA, but similar to that of 3.0 g TXA. Therefore, it was recommended that 2.0 g TXA is used as a proper dose to decrease the postoperative drainage volume of THA.
2017 Vol. 14 (30): 74-77 [Abstract] ( 275 ) HTML (1 KB)  PDF (626 KB)  ( 127 )
78 Clinical effect of open reduction and locking plate internal fixation and half shoulder joint replacement in the treatment of proximal humeral fractures in elderly patients
Suonan′angxiu TANG Weidong WANG Jun GUO Qiang XU Weisong DOU Tai
Objective To observe the clinical effect of open reduction and locking plate internal fixation and half shoulder joint replacement in the treatment of elderly patients with proximal humeral fractures. Methods The clinical data of 62 elderly patients with proximal humeral fractures treated in Qinghai Provincial People′s Hospital from January 2015 to June 2016 were retrospectively analyzed, 35 cases (observation group) were treated by open reduction and internal fixation with locking plate fixation, and 27 cases (control group) were treated with half shoulder joint replacement. The operation time, blood loss, Constant-Murley score and postoperative complications were compared between the two groups. Results There were no statistically significant differences between the two groups of the operation time, blood loss, Constant-Murley score and postoperative complications (P > 0.05). Conclusion For elderly patients with proximal humeral fractures, both open reduction and locking plate internal fixation and half shoulder joint replacement can achieve satisfactory therapeutic effect in the clinical application, and there are no differences in the postoperative complications, and the safety is high. Therefore both two kinds of surgical procedures are worthy of clinical application.
2017 Vol. 14 (30): 78-80,92 [Abstract] ( 609 ) HTML (1 KB)  PDF (631 KB)  ( 63 )
81 Clinical study of filiform needle combined with bone setting in the treatment of lumbar spinal stenosis
LIU Jinying1 LI Changsheng2▲ LI Donghui2 CAI Yongli1 KANG Zhi1 ZHANG Chaodong2 WANG Chunhong2
Objective To observe the clinical effect of filiform needle combined with bone setting in the treatment of lumbar spinal stenosis. Methods One hundred and thirty cases of patients with lumbar spinal stenosis treated in Department of Traditional Chinese Medicine, Sanhe Hospital of Hebei Province, and Department of Acupuncture, Sanhe Hospital of Traditional Chinese Medicine from August 2015 to January 2017 were randomly divided into treatment group and control group, with 65 cases in each group. The treatment group was given filiform needle combined bone setting, with iliform needle once daily, ten days in a course, total of two courses and bone setting once every two days, four times in a course, total of two courses. The control group was given acupuncture combined with bone setting, with acupuncture once daily, ten days in a course, total of two courses, with the same frequency of bone setting in treatment group. The curative effect was evaluated after treatment of two courses and the effective patients were followed up for 3 months. The degree of pain in the waist and legs was evaluated by the visual analogue scale (VAS). The adverse reactions were observed between the two groups. Results The rate of excellent and good in the treatment group (93.85%) was higher than that of control group (70.77%), with statistically significant difference (P ＜ 0.05). After treatment and at the end of follow-up, the scores of VAS in the two groups were lower than those before treatment, and the scores in the treatment group were lower than that of control group, with statistically significant differences (all P ＜ 0.05). No obvious adverse reactions were observed during treatment in the two groups. Conclusion The curative effect of filiform needle combined with bone setting is better than that of acupuncture combined with bone setting in the treatment of lumbar spinal stenosis. It can alleviate the pain and it is safe. Filiform needle combined with bone setting is a simple, effective and practical treatment.
2017 Vol. 14 (30): 81-84 [Abstract] ( 291 ) HTML (1 KB)  PDF (652 KB)  ( 107 )
85 Clinical study of filiform needle combined with massage in the treatment of degenerative lumbar spinal stenosis
KANG Guanghua1 LIU Haiyong1▲ LI Xuejun2 YANG Jianqiang1 GAO Fuxiang1 LI Donghui2 ZHANG Chaodong2
Objective To observe clinical effect of filiform needle combined with massage in the treatment of patients with degenerative lumbar spinal stenosis (DLSS). Methods Eighty-two cases of elderly patients with DLSS treated in Department of Traditional Chinese Medicine, Sanhe Hospital of Hebei Province, and Department of Acupuncture, Sanhe Hospital of Traditional Chinese Medicine from August 2015 to May 2017 were selected and divided into treatment group (41 cases) and control group (41 cases) by random number table. The treatment group was treated with filiform needle combined with massage. The control group was treated with acupuncture combined with massage. The two groups were all treated once daily, 10 days as 1 course, rest for 2 days during the treatment, total of three courses. All of the patients were followed up for 3-7 months. The degree pain of waist and legs was evaluated by the visual analogue scale (VAS). The subjective symptoms, daily of life, clinical signs were assessed by the Japanese orthopaedic association (JOA) score. The health-related quality of life (HQOL) was evaluated by the Chinese version standard scale SF-36. The adverse reactions were observed between the two groups. Results After treatment and at the end of follow-up, the scores of VAS in the two groups were all lower than those before treatment, and the scores of JOA were all higher than those before treatment, and the score of VAS in the treatment group was lower than that of control group, the score of JOA in the treatment group was higher than that of control group, with statistically significant differences (all P ＜ 0.05). The total effective rate in the treatment group (92.68%) was much higher than that of control group (78.05%), with highly statistically significant difference (P ＜ 0.01). After treatment and at the end of follow-up, the scores of HQOL in the two groups were higher than those before treatment, and the score in the treatment group was much higher than that of control group, with statistically significant differences (P ＜ 0.05). No obvious adverse reactions were observed between the two groups during the treatment. Conclusion The curative effect of filiform needle therapy combined with massage is better than that of acupuncture combined with massage in the treatment of DLSS, which can alleviate the pain, improve the signs and symptoms, promote the living quality, and it is safe. It is worthy of further study in large sample, so as to provide evidence for clinical treatment of DLSS.
2017 Vol. 14 (30): 85-88 [Abstract] ( 292 ) HTML (1 KB)  PDF (631 KB)  ( 108 )
89 Remediation effect of modified cross finger flap and abdominal flap to perioperative clinical index of patients with fingertip skin defect
WANG Peng
Objective To explore remediation effect of modified cross finger flap and abdominal flap treatment on patients with fingertip skin defect. Methods 166 patients with skin defect who had been treated in Langfang People's Hospita from February 2012 to August 2016 were selected as research objects. They were divided into modified group (99 cases) and abdominal group (67 cases), according to the different surgical methods. The patients in the modified group were treated by modified cross finger flap therapy, and the patients in the abdominal group were treated by abdominal flap surgery. The terms of joint range of motion, length of stay, length of operation, and functional restoration effect in perioperative follow up exanimation in the groups were compared. Results The excellence treatment rate, length of operation, length of stay, follow-up exanimation score, joint range of motion, dissatisfaction degree to repair appearance, pain sensitivity, sensory numbness and two-point discrimination test result in the modified group were superior to those in the abdominal group, with statistically significant difference (P < 0.05). Conclusion Conducting modified cross finger flap for patients with finger tip skin defect can not only improve the clinical indexes, but also accelerate recovery, compared with abdominal flap therapy.
2017 Vol. 14 (30): 89-92 [Abstract] ( 260 ) HTML (1 KB)  PDF (619 KB)  ( 124 )
93 Application of microendoscopic discectomy associated with annulus fibrosus repair in prolapse of lumbar intervertebral disc
LI Dong CHANG Zhiyong CHEN Gang LI Kaihua
Objective To investigate the application effect of microendoscopic discectomy associated with annulus repair in patients with single segment of prolapse of lumbar intervertebral disc. Methods The clinical data of 19 cases of patients with prolapse of lumbar intervertebral disc underwent microendoscopic discectomy associated with annulus fibrosus repair treated in Jiangsu Province Hospital of Traditional Chinese Medicine from May 2014 to December 2015 was analyzed retrospectively. The postoperative effects were evaluated by visual analogue score (VAS), Oswestry disability index (ODI) and MacNab standard. Results All patients completed the follow-up, and the mean follow-up was (27.30±5.24) months (18-37 months). No recurrence was found in 19 cases. The scores of VAS before operation, after operation for 3 months, after operation for 1 year and at the final follow-up were (7.11±0.86), (1.58±1.03), (1.37±0.81), (1.26±0.73) scores respectively. The scores of each postoperative time point were all lower than those before operation, with highly statistically significant differences (all P ＜ 0.01). The ODI before operation, after operation for 3 months, after operation for 1 year and at the final follow-up were (63.73±10.86)%, (22.26±7.58)%, (16.47±3.34)% and (14.21±3.67)% respectively. The scores of each postoperative time points were all lower than those before operation, with highly statistically significant differences (all P ＜ 0.01). Short term clinical efficacy was evaluated by MacNab standard: 14 cases were excellent, 4 cases were good and 1 case was better. The excellent and good rate was 94.7%. Conclusion Microendoscopic discectomy associated with annulus fibrosus repair is an effective mini-invasive therapy for the treatment of single segment of prolapse of lumbar intervertebral disc. Controlling the indications strictly is helpful to achieve the satisfactory curative effect and low recurrence rate.
2017 Vol. 14 (30): 93-96 [Abstract] ( 383 ) HTML (1 KB)  PDF (653 KB)  ( 215 )
97 The clinical application of arthroscope with limited debridement and extensive debridement in treating knee osteoarthritis
GUAN Lei TAN Yetong▲ TIAN Xiangdong ZHU Guangyu WANG Jian XUE Zhipeng YANG Chen DU Dongfeng
Objective To retrospectively analyze the clinical effects of limited debridement and extensive debridement under arthroscopy in the treatment of knee osteoarthritis (KOA), in order to discuss the clinical value of the two methods. Methods From January 2015 to December 2016, 88 patients with KOA treated by arthroscopic surgery in the Third Affiliated Hospital of Beijing University of Chinese Medicine were selected and divided into finite group (45 cases) and broad group (43 cases). The patients in the finite group were treated by limited debridement, and the patients in the broad group were treated by extensive debridement. The total effective rate, operation time and hospitalization time were compared between the two groups. Results There was no statistically significant difference between the two groups in total effective treatment (P > 0.05). The operation time and hospitalization time in the finite group were significantly shorter than those in the broad group, with statistically significant difference (P < 0.05). Conclusion The clinical effect of limited arthroscopic debridement and extensive debridement in treating KOA is similar. Limited debridement has advantages, such as shorter operation time, less trauma, rapid recovery, compared with extensive debridement; while extensive debridement cannot further improve the symptoms of patients requiring too much surgery. Therefore selective limited debridement is better than blind extensive debridement.
2017 Vol. 14 (30): 97-100 [Abstract] ( 273 ) HTML (1 KB)  PDF (648 KB)  ( 118 )
101 Preventive effect of Yiqi Huoxue Tongluo Decoction on lower extremity deep venous thrombosis in patients underwent total hip replacement
BAI Minghua1 CHENG Lei2 ZHANG Ziqiang1
Objective To observe the preventive effect of Yiqi Huoxue Tongluo Decoction in lower extremity deep venous thrombosis after total hip replacement. Methods One hundred and seventy-four patients underwent total hip replacement in Baoji Hospital of Traditional Chinese Medicine from January 2015 to April 2017 were selected as research objects, and they were randomly assigned into control group and observation group by random number table, with 87 cases in each group. The control group was given low molecular weight heparin calium by subcutaneous injection, on basis of which, the observation group was added with Yiqi Huoxue Tongluo Decoction. The parameters of coagulation function [prothrombin time (PT), thrombin time (TT), activated partial thromboplastin time (APTT), fibrinogen (FIB), D-Dimer (D-D)] and blood platelet count (PLT) were detected before operation and after operation for 1, 7, 14 days. The intraoperative blood loss, postoperative volume of drainage and total blood transfusion volume of the two groups were collected. Follow-up for 3 months after operation, the incidence of lower extremity deep venous thrombosis of the two groups was recorded. Results Through analysis of variance, the differences of PT, TT, APTT, FIB, D-D and PLT at different time points were statistically significant (P ＜ 0.05), the treatment factors and timing showed no reciprocal action (P ＞ 0.05), the PT, TT, APTT, FIB, D-D and PLT among different treatment factors had no statistically significant differences (P ＞ 0.05). The intraoperative blood loss and total blood transfusion volume between the two groups had no statistically significant differences (P ＞ 0.05). The postoperative volume of drainage in the observation group was less than that of control group, the difference was statistically significant (P ＜ 0.05). During follow-up, 7 cases in observation group and 5 cases in control group failed to finish the study. The incidence of lower extremity deep venous thrombosis in the observation group was 21.25% (17/80), which was lower than that of control group [35.37% (29/82)], the difference was statistically significant (P ＜ 0.05). Conclusion Yiqi Huoxue Tongluo Decoction has significant anticoagulation, thrombolysis effects, which can significantly improve clinical symptoms, reduce postoperative volume of drainage, decrease the incidence of lower extremity deep venous thrombosis after total hip replacement, it is worthy of clinical promotion and application.
2017 Vol. 14 (30): 101-104 [Abstract] ( 275 ) HTML (1 KB)  PDF (624 KB)  ( 147 )
105 Analysis on clinical application of blood component transfusion in obstetric perinatal period
HE Feiyan1 XU Li2▲ XU Hengshi3
Objective To guide timely and rational use of blood in clinic through the analysis of the application of the blood in the obstetric perinatal period. Methods The clinical data of 31 patients who delivered and received perinatal transfusion in Shanghai Ninth People′s Hospital, Shanghai JiaoTong University School of Medicine from October 2013 to February 2017 was analyzed, and the utilization of blood components was retrospectively analyzed. Results The main infusion of blood components was red blood cell products, and the blood transfusion was mainly caused by uterine atony. Conclusion The blood component transfusion can contribute to effective utilization of the blood, improve the coagulation function and stop the bleeding, which is the key measure for maternal treatment. Besides, paying attention to prenatal examination and standardized diagnosis and treatment, and grasping the opportunity of blood transfusion and reasonable utilization of blood may contribute to cutting down the rate of blood transfusion, reducing the amount of blood transfusion, and reducing the maternal mortality.
2017 Vol. 14 (30): 105-107 [Abstract] ( 231 ) HTML (1 KB)  PDF (475 KB)  ( 137 )
108 The clinical significance and changes of serum sB7-H3 and related cytokines in children with Mycoplasma Pneumoniae pneumonia
XIN Sufang1 LI Limin1 BIAN Li2
Objective To explore the relationship between serum sB7-H3 and related cytokines, and to evaluate its process in the development of Mycoplasma Pneumoniae pneumonia (MPP) through the analysis of the expression of serum soluble B7-H3 (sB7-H3) and related cytokines in MPP. Methods 110 cases children with MPP in the Fourth Hospital of Hebei Medical University from January 2016 to January 2017 were selected as the MPP group and divided into severe MPP group (54 cases), mild MPP group (56 cases) according to the condition of children. In addition, healthy children were selected as the normal control group (38 cases). The levels of sB7-H3, IFN-γ, GM-CSF, IL-4 and IL-17 in serum of MPP were detected by automatic microplate reader. Results The levels of sB7-H3, IFN-γ, GM-CSF and IL-17 in the serum of MPP children was significantly higher than those in the recovery phase and the control group (P < 0.05). The expression level of IL-4 in acute phase was lower than that in recovery phase and control group (P < 0.05). The levels of serum in the recovery phase of the MMP group was significantly higher than those in the control group (P < 0.05). IL-4 levels was lower than those in the control group. The levels of sB7-H3, IFN-γ, GM-CSF and IL-17 in the serum of MPP group were significantly higher than those of mild MPP (P < 0.05). There was no statistical difference in IL-4 levels between the two groups (P > 0.05). Serum level of sB7-H3 was positively correlated with IFN- and GM-CSF in acute phase of MPP (r = 0.573, 0.682, all P < 0.01), but not significantly related to the levels of IL-4 and IL-17 (P > 0.05). Conclusion sB7-H3 and cytokines IFN-γ, GM-CSF, IL-4 and IL-17 play an important role in the infection of children with MPP and participate in the pathogenesis of MPP.The levels of them is related to the severity of the disease.
2017 Vol. 14 (30): 108-111 [Abstract] ( 300 ) HTML (1 KB)  PDF (532 KB)  ( 114 )
112 Correlation between expression of IGF-1 and IGF-2 in placenta and the occurrence of gestational diabetes mellitus
CAI Manhong CHENG Qing▲
 Objective To explore the correlation of gestational diabetes mellitus (GDM) macrosomia with the expression of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-2 (IGF-2). Methods Forty neonates with macrosomia given birth by primiparas who were admitted to Department of Obstetrics of the Affiliated Maternity Hospital of Nanjing Medical University from April 2014 to December 2016 and confirmed with GDM were assigned to the GDM macrosomia group, 40 neonates with macrosomia given birth by healthy primiparas over the same period were assigned to the healthy macrosomia group, and 40 neonates with normal weigh given birth by healthy primiparas over the same period were assigned to the control group. The expression of IGF-1 and IGF-2 in the neonate′s placenta was compared between the three groups, and the correlation of IGF-1 and IGF-2 expression with the development of GDM macrosomia was analyzed. Results Expression of IGF-1 and IGF-2 mRNA in placenta was significantly higher in GDM macrosomia group than that in healthy macrosomia group and control group (P < 0.01), and there were no statistically significant differences between healthy macrosomia group and control group in the levels of IGF-1 or IGF-2 mRNA (P > 0.05); binary Logistic regression analysis identified IGF-1 and IGF-2 levels as the risk factors for macrosomia in GDM (OR = 3.490, 95%CI: 2.537-6.928, P = 0.017; OR = 2.077,95%CI: 1.098-4.997, P = 0.023). Conclusion Expression of IGF-1 and IGF-2 in placenta is significantly elevated in patients with GDM macrosomia; expression of IGF-1 and IGF-2 is significantly correlated with the development of GDM macrosomia.
2017 Vol. 14 (30): 112-114,128 [Abstract] ( 464 ) HTML (1 KB)  PDF (843 KB)  ( 206 )
115 The meta analysis of the clinical effects of Flurbiprofen axetil preemptive analgesia combined Propofol anesthesia on painless abortion
ZHANG Heng RAO Kun-lin▲ HUANG Junmin
Objective To systematically evaluate the clinical effectiveness and safety of Flurbiprofen axetil preemptive analgesia combined Propofol anesthesia on painless abortion. Methods The databases of PubMed, CNKI, Wanfang and VIP were retrieved from the inception to Mar 2017. The literature search was conducted using the following terms: "flurbiprofen "OR" flurbiprofen axetil" AND "preemptive analgesia" AND "induced abortion" OR "abortion technique" OR "abortion". Meta analysis was conducted by Stata 12.0 software after the data was extracted. The pooled results expressed as odds ratio and weighted mean difference, with 95% confidence interval, respectively. The fixed-effect or random-effect model was performed according to the heterogeneity. Results 14 literatures and involved in 1261 patients were analyzed. Meta analysis showed that the patients with Flurbiprofen axetil preemptive analgesia was significantly associated with shorter induction time (WMD=-1.87, P=0.02), lower intraoperative MAP (P=0.04), postoperative MAP (P=0.00), intraoperative HR (P=0.00) and postoperative HR (P=0.03).And treatment with Flurbiprofen axetil preemptive analgesia also decreased the Propofol induced pain (OR=0.56, P=0.04), intraoperative patient writhe (OR=0.23, P=0.00), respiratory depression (OR=0.10, P=0.00) and Propofol dos (WMD=-31.16, P=0.00). The shorter awakening time (WMD=-1.54, P=0.00) and the lesser incidence of  postoperation nausea (OR=0.56, P=0.04), uterus contraction pain (OR=0.15, P=0.00) and postoperation visual analogue scale (WMD=-2.18, P=0.00) was found in patients with Flurbiprofen axetil preemptive analgesia than the patients in control group. Conclusion This meta analysis suggests that Flurbiprofen axetil preemptive analgesia combined Propofol anesthesia on painless abortion is a safe and effective approach based on current evidence. But further randomized controlled trials should be undertaken to confirm these findings.
2017 Vol. 14 (30): 115-120 [Abstract] ( 310 ) HTML (1 KB)  PDF (1242 KB)  ( 163 )
121 Effect of endoscopic ketoconazole sliver combined with auditory canal irrigation on the otitis externa mycotic
LU Yayan1 LYU Jin2 WANG Xi1 ZHANG Meiguang1 WANG Weiqian1 XU Jincao1
Objective To observe the effect of endoscopic ketoconazole sliver combined with auditory canal irrigation on otitis externa mycotic. Methods 126 patients with otitis external mycotic were consecutively collected in the General Hospital of the PLA Rocket Force from May 2015 to May 2017. They were divided into two groups by random number table, with 64 cases in each goup. The patients in the control group were treated by endoscopic eloson sliver treatment for 7 days, and the patients in the experimental group were treated by ketoconazole sliver treatment combined with endoscopic ear canal irrigation for 7 days. The effect of two groups were observed. Results In the experimental group, 54 cases were cured, 8 cases were developed, 2 cases were effective and 0 case was invalid; in the control group, 38 cases were cured, 11 cases were developed, 10 cases were effective and 5 cases were invalid. The effect of the experimental group was better than that of the control group, and the difference was highly statistically significant (P < 0.01). Conclusion Higher cure rate could be obtained with endoscopic ketoconazole sliver combined with auditory canal irrigation to otitis externa mycotic.
2017 Vol. 14 (30): 121-123 [Abstract] ( 275 ) HTML (1 KB)  PDF (626 KB)  ( 180 )
124 Investigation of psychological distress in patients with sudden heaing loss and its path analysis of influencing factors
SUN Jianhua XIE Hui LIN Saimei KONG Zhe
Objective To investigate the psychological distress of patients with sudden hearing loss (SHL), in order to explore the influence path of social support and coping style on psychological distress. Methods 108 patients with SHL treated in Guangdong Provincial Hospital of Traditional Chinese Medicine from Angust to December in 2016 were investigated by kessler psychological distress scale (Kessler-10), MOS social support scale (MOS-SSS) and simple coping style questionnaire (SCSQ). Results The psychological distress score of patients with SHL was (28.85±5.88) score, more than 99% of the patients had different degrees of psychological distress; family income, the degree of hearing loss, each dimension of social support and coping style were the influence factors of psychological distress, which account for 77% of the variance; further the implementation of multiple regression analysis, social support and coping style could direct effect on psychological distress, and social support indirectly affect psychological distress by coping style. Conclusion There are widespread psychological distress in patients with SHL. Clinical doctors and nurses can carry out targeted intervention nursing according to the role of social support and coping style, so as to improve their mental health.
2017 Vol. 14 (30): 124-128 [Abstract] ( 747 ) HTML (1 KB)  PDF (599 KB)  ( 116 )
129 Clinical effect of autologous bone graft combined with Bio-OSS bone meal in bone defect after jaw cyst
YU Wei XIANG Le▲
Objective To investigate the clinical effect of autologous bone graft combined with Bio-OSS bone meal in patients with bone defect after jaw cyst. Methods From March 2014 to December 2015, 60 patients with jaw cysts treated in the Department of Stomatology, the People′s Hospital Affiliated to Hubei Medical College were divided into control group (30 cases) and observation group (30 cases) by random number table. The control group was treated with Bio-OSS bone meal. The observation group was treated with autologous bone grafting on the basis of the control group. CT examination was performed at different time points before and after operation. The CT values and difference values in bone graft area were calculated, and the clinical efficacy and incision healing were compared. Results The healing rate in the observation group was better than that in the control group (P < 0.05). The CT values of the observation group were higher than those of the control group at 3, 6 and 9 months after operation (P < 0.05). The difference values of the observation group werre lower than those of the control group at 3, 6 and 9 months after operation (P < 0.05). Conclusion Autologous bone graft combined with Bio-OSS bone meal is effective in the postoperative bone defect of jaw cysts, which is helpful for incision healing and improving the CT value and difference value. It is worthy to be popularized.
2017 Vol. 14 (30): 129-131,135 [Abstract] ( 323 ) HTML (1 KB)  PDF (747 KB)  ( 79 )
132 Research of the content of total saponins in commercially available Paris polyphylla from Wudang Mountain Area
HU Huaiming1,2 SHEN Ting3 YE Fang1,2 HUANG Liangyong1,2 LIANG Wenbin1,2
Objective To compare the content of total saponins in commercially available Paris polyphylla from Wudang Mountain Area to investigate the quality of herbal pieces purchased from hospitals, pharmacies and other pharmaceutical markets. It provides a reference for production, distribution, clinical application and regulation of Paris polyphylla. Methods Herbal pieces of the Paris polyphylla were collected from hospitals and pharmacies in Wudang Mountain Area (mainly in Shiyan, Hubei Province). The content of total saponins in Paris polyphylla was determined by an ultraviolet-visible spectrophotometer at 406 nm with perchloric acid as chromogenic reagent. Results The content of saponins in Paris polyphylla from 23 samples had obvious differences, the minimum was 1.79%, and the maximum was up to 11.79%. Conclusion The quality of Paris polyphylla is unstable which can affect the safety and efficacy of clinical application. The supervision of the production and marketing of Paris polyphylla need to be strengthened. Only by promoting the standardized planting of Chinese medicine materials, the stable quality of Paris polyphylla can be ensured.
2017 Vol. 14 (30): 132-135 [Abstract] ( 216 ) HTML (1 KB)  PDF (449 KB)  ( 160 )
136 Determination of narural vitamin E in Natural Vitamin E Soft Capsules
XU Wenfeng XU Shuo HE Xiaorong JIANG Wenqing WU Xuejun KUANG Yongmei JIN Pengfei
Objective To establish a high performance liquid chromatography (HPLC) method for the determination of narural vitamin E in Natural Vitamin E Soft Capsules. Methods An Alltima C18 column (150 mm× 4.6 mm, 5 μm) was used for the separation, with methanol as the mobile phase at the flow rate of 1.0 mL/min. The detection wavelength was 285 nm. Results The method showed good linearity with correlation coefficient of 0.9999 for vitamin E. The assay method showed good specificity. The precision and stability were satisfactory with the relative standard deviations (RSD) of 0.17% and 0.87% respectively. The average spiked recovery was 100.8% (n = 6), the recovery rate was good. The determination results was 101.2%, 100.8% and 102.7% of the labeled amount of the three batches of samples respectively. Conclusion The established method is accurate, simple and fast, and can be used for determination of narural vitamin E in Natural Vitamin E Soft Capsules.
2017 Vol. 14 (30): 136-138,167 [Abstract] ( 322 ) HTML (1 KB)  PDF (941 KB)  ( 133 )
139 Clinical study of Guan Yanling Granules in the treatment of non-erosive reflux disease with syndrome of stomach heat and qi stagnation
HUANG He HUANG Fubin ZHANG Yang LIU Zhucheng QIU Gan
Objective To study the clinical efficacy of Guan Yanling Granules in the treatment of non-erosive reflux disease (NERD) with syndrome of stomach heat and qi stagnation. Methods Eighty-six NERD patients with syndrome of stomach heat and qi stagnation admitted to Yancheng Hospital of Traditional Chinese Medicine from January 2015 to January 2017 were selected. According to the random number table method, the patients were divided into the observation group and the control group, with 43 cases in each group. The control group was treated with Omeprazole Capsules, and the observation group was treated with Guan Yanling Granules on basis of the control group. Both groups were treated for 4 weeks. The treatment effect of TCM symptoms, changes in the scores of reflux disease questionnaires, scores of clinical symptoms (regurgitation, fullness of gastral cavity, dry and bitter taste, paraesthesia pharynges, chest distress and chest pain, annoyance and irritability, heartburn), adverse reactions, and the levels of serum Ghrelin and lipid peroxide (LPO) in the two groups were observed. Results After treatment, the RDQ scores of the two groups were significantly lower than those before treatment, and the RDQ scores of the observation group were significantly lower than those of the control group, the difference was statistically significant (P ＜ 0.05). The total effective rates of TCM symptoms in the observation group and the control group were 86.05% and 55.81%, the difference was statistically significant (P ＜ 0.05). The serum Ghrelin levels of the two groups were significantly higher than those before treatment, and the levels of LPO were significantly lower than those before treatment, the differences were statistically significant (P ＜ 0.05). After treatment, the scores of clinical symptoms in the observation group were significantly lower than those before treatment, the differences were statistically significant (P ＜ 0.05), in addition to dry and bitter taste, the other symptoms in the observation group had statistically significant differences compared with those of control group (P ＜ 0.05). In the observation group, 2 cases had slight diarrhea, and 3 cases in the control group suffered from abdominal distention. Conclusion Guan Yanling Granules in the treatment of patients with NERD has significant effects and no obvious adverse reactions, which is worthy of clinical attention.
2017 Vol. 14 (30): 139-142 [Abstract] ( 212 ) HTML (1 KB)  PDF (460 KB)  ( 139 )
143 Effects of Danshen Chuanxiongqin Injection for the indexes of hemorheology, blood coagulation and oxidative stress after treatment with hyperbaric oxygen in elderly patients with cerebral embolism
LIN Xiaoyan1 BAI Jin′e1 WANG Xingning2
Objective To investigate the efficacy of Danshen Chuanxiongqin Injection for the indexes of hemorheology, blood coagulation and oxidative stress after treatment with hyperbaric oxygen in elderly patients with cerebral embolism. Methods Eighty-six patients with cerebral embolism receiving treatment with hyperbaric oxygen in Affiliated Hospital of Yan′an University from January 2016 to January 2017 were randomly divided into observation group and control group by random number table method, with 43 cases in each group. All patients were given hyperbaric oxygen therapy, on basis of which, the observation group was added with Danshen Chuanxiongqin Injection. The treatment time of the two groups was 15 days. After treatment, the scores of national institutes of health stroke scale (NIHSS) and Barthel scores in the two groups were recorded, and the clinical efficacy was evaluated. Before and after treatment, the levels of blood coagulation indexes (prothrombin time, activated partial thromboplastin time, thrombin time, international normalized ratio), hemorheology indexes (whole blood high shear viscosity, plasma viscosity, haematocrit, fibrinogen, platelet adhesion rate), and oxidative stress indexes (superoxide dismutase, methane dicarboxylic aldehyde, total antioxidant capacity, glutathione peroxidase) in the two groups were detected. Results The scores of NIHSS in the observation group were lower than those of control group, Barthel scores were higher than those of control group (P ＜ 0.05). The total effective rate of observation group was higher than that of control group (P ＜ 0.05). After treatment, the levels of blood coagulation indexes, hemorheology indexes and oxidative stress indexes in the two groups were significantly improved compared with those before treatment, and the improvement of observation group was better than that of control group (P ＜ 0.05). Conclusion Danshen Chuanx?鄄iongqin Injection combined with hyperbaric oxygen in the treatment of cerebral embolism has better effects, which can significantly improve the hemorheology indexes, and it is worthy of further exploration.
2017 Vol. 14 (30): 143-146,175 [Abstract] ( 238 ) HTML (1 KB)  PDF (965 KB)  ( 147 )
147 Effects of Shuxuetong Injection on serum inflammatory factors and platelet activating factors in patients with acute cerebral infarction
LI Xiang1 CAO Yan2 QU Lei3
Objective To study the effects of Shuxuetong Injection on serum inflammatory factors and platelet activating factors in patients with acute cerebral infarction (ACI). Methods Sixty patients with ACI admitted to Beijing Shijingshan Hospital from April 2015 to December 2016 were randomly divided into the conventional treatment group and the study group, with 30 cases in each group. The conventional treatment group was treated with the conventional western medicine, on basis of which, the study group was treated with Shuxuetong Injection. The clinical effects of the two groups were compared. The national institute of health neurological impairment scale (NIHSS) and Barthel index (BI) were evaluated. The serum inflammatory factors and platelet activating factors were examined before and after treatment. Results The total efficacy rate of the study group was 93.33%, which was significantly higher than 73.33% of the conventional treatment group (P ＜ 0.05). After treatment, the NIHSS scores of the two groups were significantly reduced, BI scores were significantly increased, and the improvement of NIHSS, BI in the study group was significantly better than that of conventional treatment group, the differences were all statistically significant (all P ＜ 0.05). After treatment, the levels of serum inflammatory factors and platelet activating factors in the two groups were all significantly decreased, and the levels of serum inflammatory factors and platelet activating factors in the study group were significantly lower than those in the conventional treatment group, the differences were all statistically significant (all P ＜ 0.05). Conclusion Shuxuetong Injection for patients with acute cerebral infarction has a good efficacy, which can improve the nervous function and daily life, reduce the serum inflammatory factors and platelet activating factors, and it is worthy of clinical promotion and application.
2017 Vol. 14 (30): 147-150 [Abstract] ( 237 ) HTML (1 KB)  PDF (458 KB)  ( 184 )
151 Systematic evaluation on the curative effect of traditional Chinese medicine combined with chemotherapy for colorectal cancer after radical operation
LIU Ningning MIAO Wenrong ZHU Huirong LI Qi▲
Objective To evaluate the clinical effect of traditional Chinese medicine combined with chemotherapy in the treatment of patients with colorectal cancer after radical resection. Methods Computer was used to search various domestic and foreign medical journals published from January 1995 to January 2015, including China biomedical literature database (CBM), China national knowledge infrastructure (CNKI), Wanfang database, China scientific journal database (VIP), PubMed, ISI Web of Knowledge, Cochrane Library and important Chinese conference paper full-text database. The quality of the selected literature was determined according to the inclusion and exclusion criteria. The quality evaluation standard provided by the Cochrane manual was referenced for quality evaluation with inclusion criteria. RevMan 5.3 software was conducted for statistical analysis. Results A total of 12 articles were included in this study. The results showed that the application of traditional Chinese medicine combined with chemotherapy could improve the quality of life (QOL) (RR = 1.76, 95%CI: 1.35-2.30, P ＜ 0.0001), increase the 5-year survival rate (RR = 1.72, 95%CI: 1.18-2.49, P = 0.005); raise CD3+, CD4+ cell count, CD4+/CD8+ ratio (MD = 18.95, 95%CI: 0.09-37.82, P ＜ 0.05; MD = 5.87,95%CI: 2.25-9.49, P = 0.00; MD = 0.24, 95%CI: 0.11-0.38, P = 0.00), decrease the incidence of gastrointestinal reactions(RR = 0.51, 95%CI: 0.42-0.61, P ＜ 0.000 01), heighten the leukocyte levels (RR = 0.51, 95%CI: 0.31-0.83, P = 0.007). Conclusion Traditional Chinese medicine combined with chemotherapy can improve the long-term curative effect of radical resection of colorectal cancer, reduce the side effects of chemotherapy, improve the quality of life and promote the immunity of the patients.
2017 Vol. 14 (30): 151-156 [Abstract] ( 722 ) HTML (1 KB)  PDF (871 KB)  ( 252 )
157 Effect of continuous pharmaceutical care on anticoagulation in a patient with VTE
TAN Hongyang1 WAN Jianwei2
Clinical pharmacists played active roles in the safety and effectiveness of anticoagulant therapy in a patient with VTE, by means of continuous pharmaceutical care. When the patient was hospitalized, clinical pharmacists participated in the formulation of initial therapeutic schedule and carried out pharmaceutical care. Then clinical pharmacists assisted physicians to develop individualized drug regimen, according to the anticoagulant effect and patient′s medication situation. After the patient was stable and discharged, clinical pharmacists offered regular follow-up, and assisted the physician to adjust the medication regimen again according to the patient′s follow-up situation, so that the international normalized ratio of the patient could reach the standard and maintain stability.
2017 Vol. 14 (30): 157-160 [Abstract] ( 248 ) HTML (1 KB)  PDF (366 KB)  ( 169 )
161 Treatment of lumbar disc herniation with tiaojitongdu by needle knife
WU Ping1 DING Deguang2
Lumbar disc herniation (LDH) is a common disease, has a certain disability. Needle knife therapy is a effective mean, but operate differently, imbalance biomechanical is usually taken to be the sally port, while there are less studies about dumai. Thus, this paper draws forth the new understanding for the etiology and pathology from the aspects of tiaoji and tongdu, points out that LDH is closed to disorder spine internal environment and imbalance biomechanical, the basic pathogenesis is "dumai stasis and yangqi losing transports". The guidance for how to choose operating position of needle knife, how to use needle knife therapy in clinic, compared with the normal needle knife, tiaojitongdu needle knife reestablish the balance of the spine, change the environment and anatomical structure of the diseased tissue, dredge dumai, restore yangqi, effectively improve the pain symptoms and the ability of the waist activity, simple operation and satisfactory effects.
2017 Vol. 14 (30): 161-163 [Abstract] ( 250 ) HTML (1 KB)  PDF (701 KB)  ( 75 )
164 Establishment and management of birth-defect biobank
JIANG Yan1* LI Lu2* WU Yulin1 LIN Ning1 XU Haoqing1
Birth defects samples are collected from primary maternal and child health care and family planning service sites, the relevant information of service objects collected and the samples detected. Designing biobank management software, encoding storage space, using the management software to manage large sample of standardized processing, store in planned space. This article discusses the work experience of birth-defect biobank establishment based on the basic level, in order to provide service for grass-roots and accumulate sample resources for research. Jiangsu Family Planning Science and Technology Research Institute has ten thousands of blood samples and DNA extractions of screening for Down symdrome, deafness, noninvasive prenatal screening, congenital heart disease, preliminarily establishes a standardized birth-defect biobank, put biobank management software into birth-defect prevention and research, which lay the foundation for the continued study.
2017 Vol. 14 (30): 164-167 [Abstract] ( 302 ) HTML (1 KB)  PDF (707 KB)  ( 98 )
168 Exploration of innovation and entrepreneurship training approaches of pharmaceutical majors in local universities
YIN Deming1 SHAN Yuxin2
Under the background of "internet+" and the big health industry, innovation and entrepreneurship talents of pharmaceutical majors are trained to adapt to the development of market economy through the appropriate transformation and development pathway at the local universities in the study. According to directional function of market development, two pharmaceutical majors including undergraduate and higher vocational are designed. The cultural atmosphere of Chinese medicine is highly shaped and students′ healthy personality is respected on the student-centered education mode. Specialized courses are mainly guided by practice cases and project tasks, and practical teaching is moreover studied the connotation of the course teaching in the factory. Pharmaceutical enterprise resources are fully used and deeply integrated with practice teaching, which drove the sustainable profession development. Both the construction of double-quality teachers are trained and the construction of specialty characteristics and advantages is featured. The evaluation system of examination and appraisals of talent development quality are reformed, and application-oriented technical talents with innovative and entrepreneurial ability is mainly focused on process and results.
2017 Vol. 14 (30): 168-171 [Abstract] ( 285 ) HTML (1 KB)  PDF (784 KB)  ( 89 )
172 Effect of community individual service plan on rehabilitation of patients with schizophrenia in Huizhou City
WANG Fengrui1 LI Penghui1 WANG Lihui2 HUANG Qunming1
Objective To explore the impact of community-based individual service plan (ISP) on the disease situation and life quality of patients with schizophrenia in Huizhou, in order to provide evidence for the promotion of ISP management. Methods 396 cases of patients with schizophrenia in Department of Psychiatry, the Second People′s Hospital of Huizhou City and Demobilized Veterans′ Hospital of Huizhou City from January 2012 to December 2015 were selected as the observational group, and given community-based ISP. Another 350 patients with schizophrenia from January 2009 to December 2011 were selected as the control group, and given conventional community management. The positive and negative symptoms scale (PANSS) and general quality of life inventory (GQOL-74) were used to assess the disease situation and quality of life of the patients in the two groups before intervention and 12 months after intervention. Results Before intervention, there were no significant differences in items scores of PANSS (P > 0.05). After intervention, the items scores of PANSS were significantly lower than before intervention, with statistically significant differences (P < 0.05). After intervention, scores of negative symptom, positive symptom and total item of patients in the observational group were significantly lower than those in the control group, with statistically significant differences (P < 0.05). Before intervention, there were no significant differences in items scores of GQOL-74 (P > 0.05). After intervention, the items scores of GQOL-74 were significantly higher than before intervention, with statistically significant differences (P < 0.05). After intervention, scores of physical function, psychological function, social function of patients in the observation group were significantly higher than those in the control group, with statistically significant differences (P < 0.05). Conclusion The implementation of ISP management model can improve the condition of patients with schizophrenia, improve their quality of life and social performance.
2017 Vol. 14 (30): 172-175 [Abstract] ( 251 ) HTML (1 KB)  PDF (713 KB)  ( 95 )
176 Analysis on application of proton-pump inhibitor of 259 patients during the perioperative period in our hospital
ZHANG Li WANG Yijun
Objective To investigate the application of proton pump inhibitors (PPIs) in patients during the perioperative period in the Second Affiliated Hospital of Nanjing Medical University (“our hospital” for short), analyze the irrational phenomenon, in order to provide reference for rational drug use. Methods 259 patients during the perioperative period used PPIs were chosen from July 2016 to January 2017 in several departments of our hospital, and their PPIs application situation were analyzed. Results All the PPIs were given by intravenous administration. 165 (63.7%) cases had no risk of stress ulcer. 137 cases (52.9%) had irrational of drug frequency. The average duration was 4.59 d. The consumption sum of PPIs accounted for 5.63% of the total drug consumption sum and 1.98% of the total hopspitalization cost, among which, the consumption sum of PPIs in patients with no risk factors for stress ulcer accounted for 47.7% of the total consumption sum for PPIs. Conclusion There is high rate of irrational perioperative use of PPIs in our hospital, including irrational of drug frequency and duration. More powerful management should be given, in order to regulate the use of PPIs.
2017 Vol. 14 (30): 176-179,184 [Abstract] ( 247 ) HTML (1 KB)  PDF (807 KB)  ( 184 )
180 Community management of screening and intervention of high-risk population of stroke under the guidance of medical partnerships system and its effectiveness evaluation
ZHANG Pengchuan SONG Wei WANG Haiying HE Shaoqi ZHAO Ya′nan XIE Xiujuan
Objective To study the community management of screening and intervention of high-risk population of stroke under the guidance of medical partnerships and evaluate its effectiveness. Methods From January to December 2015, Demao Community and Hongxing Building Community in Daxing District of Beijing were selected as study bases. 200 cases with high-risk of stroke aged 41-91 were collected, and randomly divided into the intervention group (100 patients) and the control group (100 patients). 11 doctors from the two communities had been trained for standard of high-risk population of stroke screening and intervention pilot project in a tertiary general hospital that under the medical partnerships system. Patients in the intervention group were given follow-up management, patients in the control group were given no intervention. The main follow-up indicators for community doctors included awareness rate of screening and intervention in high-risk population of stroke, positive rate of screening, acceptance degree of standardized treatment; for patients included awareness rate of risk factors of stroke, medication rate, use rate of standardized drugs, self-daily life management rate. Results After standard training of high-risk population of stroke screening and pilot intervention, the awareness rate of community doctors on screening and intervention in high-risk population, the screening positive rate and the acceptance degree of standardized treatment were raised than before(P < 0.05). In the intervention group 96 cases had effective follow-up, and in the control group 97 cases had effective follow-up, the follow-up time was (18.5±0.5) months. The awareness rate of stroke risk factors, medication rate, rate of accepting standardized drugs and self-daily life management rate in the intervention group were higher than those in the control group (P < 0.05). Conclusion The community management model of screening and intervention in high risk population of stroke under the guidance of a tertiary general hospital that enrolled in medical partnerships system, improves the diagnosis and treatment levels of community doctors which in turn can be beneficial to recognizing and well-management of high-risk population, as well as the awareness rate and execution degree of management control program of community disease. It also improves the awareness rate and self-management level of patients with high risk factors, which contribute to patients′ medication rate and medical compliance, thus provides a new method for medical and health reform.
2017 Vol. 14 (30): 180-184 [Abstract] ( 629 ) HTML (1 KB)  PDF (799 KB)  ( 111 )
185 Reflection on constructing the harmonious doctor-patient relationship in university hospital
YIN Xiuxiu LIU Xiaofeng XING Yu′e CHEN Hongyan
The country's education and medical level are improving constantly. However, the current doctor-patient relationship is in a state of tension. The university hospital is no exception. This article takes the present situation of university hospital as the starting point. The reasons are analyzed from the aspects of medical environment, hospital, medical personnel and patients. The concrete measures to construct harmonious doctor-patient relationship are put forward. The following aspects are covered: establish the orientation and development direction of university hospital, improve the medical technical level of medical staff, perfect medical facilities construction and strengthen the communication between doctors and patients.
2017 Vol. 14 (30): 185-188 [Abstract] ( 218 ) HTML (1 KB)  PDF (708 KB)  ( 128 )
189 Analysis of irrational Chinese patent medicine prescription in outpatient department
KANG Xue XING Shuang XU Baohai
Objective To discuss the prescription of irrational Chinese patent medicine in outpatient department and put forward measures. Methods Based on Hospital prescription review management standard, Clinical application guidelines of Chinese patent medicine and the drug instructions. The prescriptions of irrational Chinese patent medicine in the outpatient from July 2016 to July 2017 were classified and analyzed. Results The irrational use of drugs was summed up as follows: inconsistent of diagnosis and prescriptions, unreasonable of combination, no signature of doctor, usage error, wrong prescription color, ultra-maximum dosage of instructions, inappropriate dosage of children, extraordinary prescription, contraindications, ultra-limit and repeated medication. The larger proportion departments in unreasonable use of drugs were mainly TCM department, hand surgery department, cardiology, orthopaedic orthopaedics, cadre branch and so on. Conclusion The pre-audit intervention of prescription is of great significance to the safety of patients, the doctors and pharmacists should constantly improve the level of knowledge about traditional Chinese medicine, measures of unreasonable prescription surveillance should improve and regulate the rational use of traditional Chinese medicine.
2017 Vol. 14 (30): 189-192 [Abstract] ( 342 ) HTML (1 KB)  PDF (707 KB)  ( 184 )
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