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| Role of UNR in regulating the epithelial mesenchymal transition of epithelial ovarian cancer cells |
| WENG Jianrong MIAO Yi SUN Yunyan |
| Department of Gynecology and Obstetrics, the First People′s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200080, China |
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Abstract Objective To detect the expression of UNR gene in epithelial ovarian cancer cells, and to observe the effect of UNR gene on epithelial mesenchymal transition (EMT) of ovarian cancer cells. Methods Real-time quantitative PCR (qPCR) was used to detect and compare the expression of UNR gene in human epithelial ovarian cancer cell lines SKOV-3, HO-8910, ES-2 and immortalized human ovarian epithelial cell lines MOODY in vitro. qPCR and Western blot were used to detect the UNR-RNAi interfering efficiency of ovarian cancer cell SKOV-3, the invasion ability of SKOV-3 cells was detected by Transwell invasion assay. The protein expression of E-cadherin in SKOV-3 cells was detected by Western blot. Results UNR was significantly expressed in the epithelial ovarian cancer cell lines SKOV-3, HO-8910 and ES-2. After SKOV-3 was transfected by shUNR, Transwell assay showed that the number of transmembrane cells in UNR-RNAi group was (29.33±5.14) per field, which was significantly lower than that in negative control group [(76.00±5.42) per field] (P ＜ 0.01). In addition, Western blot showed that the expression of E-cadherin protein in SKOV-3 cells was significantly up-regulated after UNR gene interference. Conclusion shUNR can markedly inhibit the expression of UNR gene in ovarian cancer SKOV-3 cells, decrease the invasive ability of ovarian cancer cells. The up-regulated expression of E-cadherin after interference shows that UNR may promote the occurrence of ovarian cancer EMT, thus, the interfering technology taking UNR gene as target is expected to become a new strategy for the treatment of ovarian cancer.
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