人参提取物对过氧化氢致人神经母瘤细胞损伤的保护作用及机制研究

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Abstract Objective To observe the influence of ginseng extract on the injury induced by hydrogen peroxide in human neuroblastoma cells (SH-SY5Y) and explore its mechanism. Methods The optimal concentrations of panaxadiol saponins (PDS), ginsenoside(GS) and ginseng non-saponin(GN) were determined by the MTT assay. SY5Y cells in the logarithmic growth phase were supplemented with hydrogen peroxide (final concentration of 300 μmol/L) and were incubated for 4 h. The cell injury model was established. SY5Y cells were divided into model control group, PDS group, GS group and GN group. A blank control group was added to an equal volume of cell culture fluid. MTT assay was used to detect the cell viability. The protein expressions of Nrf2, Keap1, Bcl-2, Bax, Caspase3 and Caspase9 were detected by Western blot. Results Compared with the blank control group, the survival rate of SH-SY5Y cells in the model control group was significantly decreased (P < 0.01). Compared with the model control group, the survival rates of PDS group, GS group and GN group increased, and the concentration of 1, 10, 240 μg/mL was selected as test concentration in PDS group, GS group and GN group (P < 0.01). Compared with the blank control group, the protein levels of Keap1, Bax, Caspase3 and Caspase9 in SH-SY5Y of model control group were significantly increased (P < 0.05 or P < 0.01), and the protein levels of Bcl-2 and Nrf-2 were significantly decreased (P < 0.01). Compared with the model control group, in PDS group and GS group the protein levels of Keap1, Bax, Caspase3 and Caspase9 were significantly decreased (P < 0.05 or P < 0.01), and the protein levels of Bcl-2 and Nrf-2 were significantly increased (P < 0.01). Conclusion PDS and GS from ginseng extract can protect human SH-SY5Y cells from the hydrogen peroxide-induced damage, and the mechanism may inhibit the oxidative stress by activating the Nrf-2 pathway in SH-SY5Y cells, and reduce apoptosis.

Key words： Panaxadiol saponins Ginsenoside Ginseng non-saponin Hydrogen peroxide Neuroblastoma injury

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	ZHANG Huiyuan YANG Qing SUN Jiaming LIU Yingna LIN Jia′nan SHI Xiaozheng LI Na QU Xiaobo

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ZHANG Huiyuan YANG Qing SUN Jiaming LIU Yingna LIN Jia′nan SHI Xiaozheng LI Na QU Xiaobo. Protective effect and mechanism of ginseng extract on human neuroblastoma injury induced by hydrogen peroxide[J]. 中国医药导报, 2017, 14(30): 13-17.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I30/13

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