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| Influence of glucose carbon source on the constitutive expression of recombinant human C-reactive protein |
| LI Junming YANG Liping WANG Chenyu ZHAO Qi ZHENG Qing SUN Chengming▲ |
| Department of Clinical Laboratory, Yantai Yuhuangding Hospital, Shandong Province, Yantai 264000, China |
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Abstract Objective To investigate the effect of different concentrations of glucose on the constitutive expression of recombinant human C-reactive protein (rhCRP) in Pichia pastoris, and to screen out the optimum concentration of the glucose carbon source. Methods The different concentrations [0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% and 3.5% (w/v)] of glucose were used to constitutively express rhCRP in Pichia pastoris. Samples were taken from individual tubes of the cultures at the desired time points and the expression of rhCRP in the culture supernatant was analyzed by Western blot and gray scale. Results When rhCRP was constitutively expressed in Pichia pastoris X-33 using glucose as carbon source, 1.0% glucose (w/v) was selected as the best carbon source. Conclusion The concentration of glucose carbon source plays an important role in the constitutive expression of rhCRP in Pichia pastoris. The results of this study will provide a strong experimental basis for further optimized expression and large-scale production of rhCRP.
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