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2019 Vol. 16, No. 8 Published: 2019-03-15

	9	Effects of different acupuncture methods in Mongolian Medicine on monoamine neurotransmitters and PKA/CREB/BDNF pathway in prefrontal lobe of depressed rats
		AI Liya1 YANG Lijuan2 LIU Juntong2 AO Qier3 Saiyinchaoketu3
		Objective To observe the effects of different acupuncture methods on the neurotransmitter and protein kinase (PKA) activity, cyclophosphamide receptor binding protein (CREB) phosphorylation and brain-derived neurotrophic factor (BDNF) expression in the prefrontal cortex of depressed rats. To explore the antidepressant mechanism of different acupuncture methods in Mongolian medicine. Methods A total of 40 male Sprague-Dawley rats were divided into blank group, model group, Mongolian medical silver needle group, Mongolian medicine three balanced needle group and drug group according to random number table method, with 8 rats in each group. Rats in the other groups were orphaned except for the blank group and were given unpredictable 7 different stress stimulation for 28 days. Among them, 4 rats in the blank group were raised in each cage and fed with normal drinking water without any stimulation; the model group did not receive any treatment; the Mongolian medical silver needle group received the silver needle for stimulation of the Bada dry point, Heart acupuncture points and black and white points 1 h before the daily stress stimulation; acupuncture and acupuncture points of the Mongolian medicine three balanced needle group were the same as those in the Mongolian medical silver needle group, but one acupoint was selected daily for warm needle stimulation; the drug group was given 1 hour before stress stimulation daily, Fluoxetine hydrochloride was formulated into a 1 mg/1 mL solution in a 0.9% sodium chloride solution, and the drug group received intragastric administration according to a body weight of 2 mg/kg. After 28 days, the rats were decapitated and the prefrontal lobe was taken. The 5-hydroxytryptamine (5-HT), norepinephrine (NE), dopamine (DA) content, PKA activity, CREB phosphorylation and BDNF expression level were detected by enzyme-linked immunosorbent assay and Western blot. Results Compared with the blank group, the contents of 5-HT, NE and DA in the prefrontal cortex of the model group were significantly decreased after 28 days of modeling (P ＜ 0.01), and the levels of PKA activity, CREB and BDNF expression were also significantly decreased (P ＜ 0.05). Compared with the model group, the contents of NE, DA and 5-HT in the prefrontal cortex of Mongolian medicine three balanced needle group and drug group were significantly increased, the differences were statistically significant (P ＜ 0.05), the contents of DA and 5-HT in the prefrontal cortex of Mongolian medical silver needle group were significantly increased, the differences were statistically significant (P ＜ 0.05). Compared with the model group, the levels of PKA activity, CREB and BDNF expression in the prefrontal cortex of Mongolian medical silver needle group, Mongolian medicine three balanced needle group and drug group were significantly increased, the differences were statistically significant (P ＜ 0.05). Conclusion Different needles of Mongolian medicine can effectively increase the content of 5-HT/NE/DA, the activity of PKA and the expression of CREB-BDNF, suggesting that different needles of Mongolian medicine have antidepressant effects.
		2019 Vol. 16 (8): 9-12,38 [Abstract] ( 245 ) HTML (1 KB) PDF (1123 KB) ( 110 )

	13	Study on the intervention effect of Buyang Huanwu Decoction on APP/PS1 double transgenic mice based on metabolic network technology
		WANG Baiyun1 YANG Liu2 LIU Guoliang2 PIAO Chengyu3 LI Qingwei2 XUE Hui2 LIU Bin2
		Objective To reveal the regulation mechanism of Buyang Huanwu Decoction on APP/PS1 double transgenic mice based on metabolomics. Methods An animal model of Alzheimer′s disease was replicated in 20 APP/PS1 double transgenic mice. APP/PS1 double transgenic mice were randomly divided into two groups: model group and Buyang Huanwu Decoction group (9.26 g/kg). Ten healthy BALB/c mice were selected as control group. Buyang Huanwu Decoction group orally administrated with Buyang Huanwu Decoction, control group and model group were given the same volume of normal saline. Water maze behavior part tested the learning and memory ability of mice after 35 days′ intragastric administration. Then, the urine metabolites profiling analysis was conducted based on the high-throughput liquid chromatography mass spectrometry. The key metabolic enzymes or metabolic pathways in the course of disease development were traced by terminal metabolites, focusing on the pathogenesis of APP/PS1 double transgenic mice and the mechanism of Buyang Huanwu Decoction. Results In the Morris space exploration experiment, compared with the blank group, the number of platform crossing in the model group was significantly reduced (P < 0.05), the number of platform crossing in the Buyang Huanwu Decoction group was significantly increased (P < 0.05), and the residence time in the target quadrant was significantly increased (P < 0.05). In the space positioning navigation experiment, the escape latency of Buyang Huanwu Decoction group was significantly shorter than that of model group (P < 0.05). Through further analysis of urine metabolic trajectory, it was found that the urine of mice in each group was clustered well, and the separation between blank group and model group was obvious, while Buyang Huanwu Decoction group was located between the two groups. Through the contribution analysis of larger difference ion, it was found that lipid metabolism and lipid metabolism were mainly distributed in sphingolipids. The above metabolic pathways showed a significant callback trend after supplementing Buyang Huanwu Decoction. Four biomarkers with abnormal metabolism were identified by high resolution mass spectrometry. They were arachidonate acid, sphingosine, L-palmitoyl carnitine and glycerophosphocholine. Through online analysis of the metastadata data processing platform Metaboanalyst 4.0, it was known that it mainly involved two metabolic pathways, namely sphingolipid metabolism and arachidonic acid metabolism. Conclusion Buyang Huanwu Decoction has obvious effects on Alzheimer′s disease mice, which can effectively improve the learning and memory ability. Regulation of amino acid metabolism and energy conversion may be key mechanisms for its regulation of Alzheimer′s disease.
		2019 Vol. 16 (8): 13-17 [Abstract] ( 186 ) HTML (1 KB) PDF (779 KB) ( 193 )

	18	Effects of artesunate on ischemia-reperfusion injury after testicular torsion/detorsion in rats
		WANG Lei WENG Xiaodong CHEN Zhiyuan LIU Xiuheng
		Objective To investigate the effects of artesunate on ischemia-reperfusion injury after testicular torsion/detorsion in rats. Methods Eighteen adult healthy male Sprague Dawley rats were divided into control group, ischemia group and artesunate group by random number table method, with 6 rats in each group. The model rats with testicular torsion/detorsion were made (720° for 2 h). The artesunate group was given artesunate 25 mg/(kg·d) by gavage administration at 3 d before operation. The control group and ischemia group were given same volume of distilled water by gavage administration. After detorsion for 4 h, the rats were sacrificed and the testicular tissue was obtained, the contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were detected, and the histopathological parameters and apoptosis index (AI) were tested by HE staining and TUNEL staining. The expression of poly (ADP-ribose) polymerase (PARP)-1 and apoptosis-inducing factor (AIF) in testicular tissue of each group was detected by Western blot. Results Compared with control group, the SOD activity and the diameter of seminiferous tubules in ischemia group and artesunate group were significantly decreased, while the MDA content, apoptosis index, the expression of PARP-1 and AIF in ischemia group and artesunate group were significantly increased, the differences were statistically significant (P ＜ 0.05). Compared with ischemia group, the SOD activity and the diameter of seminiferous tubules in artesunate group were significantly increased, while the MDA content, apoptosis index, the expression of PARP-1 and AIF in artesunate group were significantly decreased, the differences were statistically significant (P ＜ 0.05). Conclusion Artesunate has protective effects on ischemia-reperfusion injury after testicular torsion/detorsion, its mechanism may be achieved through inhibiting apoptosis and oxidative stress.
		2019 Vol. 16 (8): 18-21 [Abstract] ( 174 ) HTML (1 KB) PDF (1002 KB) ( 141 )

	22	Methylation of O6- methylguanine -DNA transferase gene promoter in bladder cancer and its clinical significance
		LIU Xiaoli1 SHAN Xiaohui2 WANG Taoxia1 FENG Shuning1 WANG Xiaoying1 DUAN Xiaoting1 LI Guiying1
		Objective To investigate the methylation of O6-methyl guanine-DNA methyl transferase (MGMT) gene promoter in bladder cancer was studied. Methods Ninety-three patients cases of bladder cancer patients admitted to the Affiliated Hospital of Hebei University of technology from January 2010 to January 2012 and treated with surgical resection were selected. Forty-seven cases of bladder cancer patients Admitted to the Hospital during the same period and confirmed to have no bladder tumor by cystoscopy were selected as the control group. Methylation of MGMT gene in bladder cancer and normal bladder mucosa were detected by methylation specific polymerase chain reaction (PCR). The correlation between MGMT methylation and clinical-pathological characteristics was statistically analyzed, and Kaplan-Meier was used to analyze the correlation between MGMT methylation and patient prognosis. Results The relative methylation of MGMT in bladder cancer tissues was significantly higher than that in normal bladder tissues, and the difference was statistically significant (P ＜ 0.01). MGMT methylation was correlated with staging and grading (all P ＜ 0.01), but not with age, gender, tumor size, number, morphology and recurrence frequency (all P ＞ 0.05), Kaplan-Meier survival analysis found that the 5-year overall survival rate of the methylated group was significantly lower than that of the non-methylated group (all P ＞ 0.05). Conclusion The methylation level of MGMT gene was significantly increased in bladder cancer tissue, and MGMT methylation was related to tumor stage, grade and poor prognosis, which could be used as a molecular marker for the diagnosis and prognosis of bladder cancer and worthy of clinical attention.
		2019 Vol. 16 (8): 22-25 [Abstract] ( 279 ) HTML (1 KB) PDF (587 KB) ( 98 )

	26	Advance of BET family and its inhibitors in tumor
		ZHOU Xiao CHENG Zhixiang
		Bromodomain and extra terminal domain (BET) family is one of bromodomain proteins, which is up-regulated in various tumors and involved in tumor pathogenesis by regulating cell growth, proliferation and differentiation, apoptosis and necrosis. Several BET inhibitors have been developed and clinically tested, the antitumor efficacy of BET inhibitor alone or in combination with existing drugs has been identified in different tumors types, so it may become a new target for tumor treatment. This paper summarizes the current knowledge of BETs and its inhibitors in tumor.
		2019 Vol. 16 (8): 26-29,46 [Abstract] ( 423 ) HTML (1 KB) PDF (617 KB) ( 158 )

	30	Research progress on post-stroke depression
		CHEN Shun PANG Tao▲
		Post-stroke depression (PSD) is the most common mental health problem, accounting for approximately 33% of stroke survivors. PSD has a negative impact on post-stroke rehabilitation, leading to cognitive deficits and a significant increase in neurovascular recurrence. Biological and psychological factors have been shown to play an important role in PSD. Risk factors and predictors of PSD have been found to be of concern in screening and diagnosis, but no corresponding testing tools have been developed. According to the patient′s basic conditions and response characteristics, drug treatment should be selected, and the importance of psychotherapy also should be emphasized. This article is based on the existing research on PSD prediction, screening and treatment, and provides reference for future related research.
		2019 Vol. 16 (8): 30-34 [Abstract] ( 187 ) HTML (1 KB) PDF (722 KB) ( 92 )

	35	Advances in microenvironment of chronic myelogenous leukemia stem cells and their response to drug therapy
		HE Ling1,2 ZHANG Xiaoyan3 YANG Yazhi2 LI Jian2▲
		Chronic myelogenous leukemia (CML) is a malignant myeloproliferative disease. With the use of chemotherapy drugs such as tyrosine kinase, improve its clinical remission rate, but drug resistance is up to 15 percent and rising every year. It was found that residual leukemia stem cells (LSCs) in bone marrow microenvironment (BM) play an important role in the acquisition of drug resistance in patients with chronic myelogenous leukemia. LSCs secrete certain cytokines, which play a role in remodeling BM and establishing protective “niches”. Among them, the cytokine CCL3 in the microenvironment can recruit Nestin+ mesenchymal stem cells (MSCs) to form a protective “niche” to shelter LSCs; then TGF-β1 converts MSCs Nestin+ to -SMA +MSCs, becoming a mature “niche” protecting LSCs. Thereby tolerating the killing of chemotherapy drugs, leading to disease recurrence. On the other hand, the bone marrow microenvironment can cause abnormalities in the signaling pathways of LSCs, epigenetic changes, and increased activity of transmembrane transport proteins through a variety of mechanisms, resulting in the “monitoring” of LSCs to escape the immune system. This article reviews the BM and LSCs mediated chronic myelogenous leukemia drug resistance.
		2019 Vol. 16 (8): 35-38 [Abstract] ( 186 ) HTML (1 KB) PDF (594 KB) ( 77 )

	39	Progress in the treatment and management of inflammatory bowel disease
		FENG Gong1 MI Man1 LI Xueping1 LIU Manling1 FAN Liping1 NIU Chunyan2
		Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the intestine with a tendency to relapse for life, which requires long-term medication and the cost is expensive. Based on the latest research progress in the treatment and management of IBD, this paper starts from several key points, involving the optimized treatment of 5-aminosalicylic acid (5-ASA) of IBD, biological agents and immunomodulatory therapy, the psychotherapy scheme, the intestinal microflora treatment scheme, and 5S principles in IBD management, in order to enhance the understanding of IBD and improve the level of treatment and management of IBD among grass-roots physicians.
		2019 Vol. 16 (8): 39-42 [Abstract] ( 228 ) HTML (1 KB) PDF (615 KB) ( 209 )

	43	Resaerch progress in the relationship between human papillomavirus infection and male reproductive health
		ZHOU Qing LIU Shuaimei ZHANG Ruijin SHI Hui LIN Ning WU Yulin XU Haoqin
		Human papillomavirus (HPV) infection is common in the population and is one of the common sexually transmitted diseases. HPV-DNA may exist on the epithelium of male and female reproductive organs, which may affect human reproductive health. Male HPV infection is associated with reproductive health problems such as abnormal semen parameters, impacting sperm DNA integrity, reducing assisted reproductive pregnancy rate, the production of anti-sperm antibodies and the formation of reproductive organ tumors. Therefore, some measures should be taken to reduce HPV infection rate and improve reproductive health, including HPV vaccination, correct use of condoms and reducing sexual partners.
		2019 Vol. 16 (8): 43-46 [Abstract] ( 215 ) HTML (1 KB) PDF (580 KB) ( 91 )

	47	Advances in the study of the mechanism of resistance of Acinetobacter baumannii to antibiotics
		MU Peng1 HU Fangfang2 YUAN Jun1,3▲
		Acinetobacter baumannii (Ab) is an important pathogens responsible for hospital-acquired infections, it is the undoubtedly one of the most successful pathogens responsible for the patients with a defective or low immune system or the ill patients in the ICU. The mechanism of multidrug resistance of 1Ab is mainly related to the hydrolysis of ndogenous enzymes to antibiotics. The reduction of affinity of antibiotics to target caused by changes in the target of agents that under the regulate of the modified enzyme. Excretion of antibiotic mediated by over-expression of efflux pump and abnormal structure of cell wall porin. The reduction of antibiotic sensitivity mediated by membrane potential abnormality that caused by permeability defect of cytomembrane. Changes of antibiotic targets. The decrease of sensitivity of antibiotics to targets mediated by changes of cell wall composition. Drug resistance mediated by enzymes dysfunction and protein synthesis abnormality that caused by insertion or deletion of endogenous or exogenous genes. In the review, we summarize current studies that contribute to the mechanism of multidrug resistance in Ab by utilizing the mechanism of various antibiotics and the virulence factors of Ab.
		2019 Vol. 16 (8): 47-50 [Abstract] ( 251 ) HTML (1 KB) PDF (595 KB) ( 94 )

	51	Progress in diagnosis and treatment of child with anemia
		ZHAO Li1 YE Fang2▲
		Anemia is a common clinical disease in child. Serious cases will affects the growth and development without being discovered, diagnosed and treated in time. The etiology of child anemia is complex. There are many differences in the pathogenesis, diagnosis, treatment and prognosis of various types of anemia. Malnutritional anemia, especially iron deficiency anemia is the most common. The younger the age, the higher the incidence. At the community hospital, blood routine testing can give a preliminary diagnosis of iron deficiency anemia. Iron supplements can improve exsanguine conditin. If the treatment is invalid, and the diagnosis is unclear, we should consider the other typs of anemia, such as anemia of inflammation, rheumatic immune disease related anemia, cancer related anemia, hemolytic anemia and chronic disease, and the patient need to be transferred to the hospital specialized in hematology for correct diagnosis and prompt treatment. This paper described the research progress in the clinical prevalence, pathogenesis, diagnosis and treatment of mulnutritional anemia, anemia of inflammation, rheumatic immune disease related anemia, cancer related anemia, hemolytic anemia, secondary anemia and provided a solid foundation for further studies of child anemia.
		2019 Vol. 16 (8): 51-54 [Abstract] ( 303 ) HTML (1 KB) PDF (589 KB) ( 140 )

	55	Study on the correlation between job burnout and job stressors of young nurses in the first-class hospital at Grade 3 of Shandong Province
		LI Jie1 YU Jun2
		Objective To investigate the job burnout of young nurses in the first-class hospital at Grade 3 of Shandong province and its correlation with job stressors. Methods From May to June 2014, 405 young nurses from Affiliated Hospital of Shandong University of Traditional Chinese Medicine, the Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Qianfoshan Hospital, Qilu Hospital of Shandong University and the Second Hospital of Shandong University were selected by the convenient sampling method, and a questionnaire survey was conducted using the nursing burnout scale and the Chinese nurses′ work-stressor scale, and its correlation was analyzed. Results Among the scores of young nurses for job burnout, the scores of somatic symptoms and influencing social activities and family life were the highest, (7.79±2.63) points and (7.70±2.69) points respectively. The dimension with the highest score of work stressors were the nature of work and workload, (37.94±9.98) points and (32.04±7.58) points respectively. There was a positive correlation between the job burnout of young nurses and the total score of job stressors and the scores of each dimension (P ＜ 0.01). Conclusion There is a significant correlation between job burnout and job stressors of young nurses.
		2019 Vol. 16 (8): 55-57,62 [Abstract] ( 191 ) HTML (1 KB) PDF (471 KB) ( 111 )

	58	Prevalence and influencing factors of anxiety and depression among hospitalized patients with cardiovascular diseases in Dalian City
		MO Wenping CHEN Li LI Na GUAN Hong
		Objective To investigate the current situation of anxiety and depression in hospitalized patients with cardiovascular diseases in different grade hospitals in Dalian, and to analyze the influencing factors. Methods A total of 1334 cases of patients with cardiovascular diseases in 20 different grade hospitals in Dalian City were investigated at the same time at 8∶00 November 15, 2017 to 8∶00 November 16, 2017 (within 24 hours). The general information questionnaire, self-rating anxiety scale (SAS), self-rating depression scale (SDS), self-management behavior scale and social support scale (SSRS) were used to investigate. Multiple linear regression analysis was used to analyze the influencing factors. Results The scores of SAS and SDS of hospitalized patients with cardiovascular diseases in Dalian were higher than those of the national norm, and the differences were highly statistically significant (P ＜ 0.01). The scores of SAS in patients with heart failure were the highest, the scores of SDS in patients with intervention were the highest. There were significant differences in SAS scores among different hospital grades, gender, occupation, education level, residence, economic status, living conditions and major economic source groups (P ＜ 0.05). There were significant differences in SDS scores among patients with different hospital grades, age, occupation, education, economic status, living conditions and major economic sources (P ＜ 0.05). SAS score was negatively correlated with total scores of self-management and SSRS (r = -0.165, -0.210, all P ＜ 0.01), SDS score was negatively correlated with total scores of self-management and SSRS (r = -0.164, -0.254, all P ＜ 0.01). The results of multiple linear regression analysis showed that the living condition, SDS score, self-management and SSRS score were the influencing factors of anxiety (P ＜ 0.05), and occupation, age, hospital grade, SAS score, self-management and SSRS score were the influencing factors of depression (P ＜ 0.05). Conclusion The level of anxiety and depression of inpatients with cardiovascular diseases in Dalian is high. Medical staff should pay more attention to the patients living alone, without stable work and in grass-roots hospital, and give targeted treatment and intervention to improve the negative mood of patients.
		2019 Vol. 16 (8): 58-62 [Abstract] ( 525 ) HTML (1 KB) PDF (452 KB) ( 94 )

	63	Heart damage caused by KCNJ5 gene mutation in patients with primary aldosteronism
		HU Junli HONG Jing WU Ting LI Juan LUO Qin WANG Guoliang WANG Menghui LI Nanfang
		Objective To analyze the genetic variation and clinical data of the inward rectifier potassium channel J subfamily member 5 (KCNJ5) in patients with primary aldosteronism, and to investigate the cardiac damage in patients with different KCNJ5 gene mutations in primary aldosteronism. Methods From January 2011 to December 2015, 10 patients with unilateral adrenal cortical hyperplasia (UAH) and 18 patients with aldosterone-producing adenoma (APA) were collected from the Hypertension Center of the People′s Hospital of Xinjiang Uygur Autonomous Region. The general clinical data, biochemical parameters, KCNJ5 genotype were recorded, and echocardiography was performed. Results Among the 128 patients, 7 patients had G151 mutation, 5 patients with L168 mutation, and 16 patients with S209 mutation, and the systolic blood pressure and aldosterone renin activity ratio (ARR) of G151 mutation were higher than those of L168 and S209 mutation, and their blood potassium levels were lower than those of L168 and S209 mutation, and the differences were statistical significant (P < 0.05). The left ventricular diastolic diameter, left ventricular posterior wall thickness and left ventricular mass (LVM) of patients with G151 mutation were higher than those of patients with other two mutation types, and their ejection fraction (EF) was lower than those of patients with L168 and S209 mutation, but there was no significant difference among the three mutations (P > 0.05). In APA patients, the left ventricular diastolic diameter and LVM of G151 mutation patients were higher than those of the other two mutation types, and there were significant difference compared with L168 mutation patients (P < 0.05). Conclusion The degree of damage of different KCNJ5 gene mutations in target aldosteronism patients may be different. The heart damage of patients with G151 mutation is more serious than that of L168 and S209.
		2019 Vol. 16 (8): 63-67 [Abstract] ( 189 ) HTML (1 KB) PDF (352 KB) ( 78 )

	68	Application of enhanced recovery after surgery in patients with hypertensive cerebral hemorrhage
		HUANG Liheng LONG Liangchun YANG Qiumin GUO Shengmin
		Objective To explore the application value and safety of enhanced recovery after surgery (ERAS) in perioperative patients with hypertensive intracerebral hemorrhage. Methods One hundred and sixty-four patients with hypertensive intracerebral hemorrhage, treated in the Affiliated Hospital of Southwest Medical University from June 2014 to June 2016 were collected. The patients were divided into traditional group (82 cases) and ERAS group (82 cases) according to random number table method. The postoperative recovery of postoperative Glasgow outcome score (GOS), activities of daily living (ADL) and Fugl-Meyer assessment scale (FMA) scores in the two groups were compared. Results There was no significant difference in Glasgow coma scale (GCS) score between the two groups at admission (P ＞ 0.05). After 1, 6 months of follow-up, the condition of GOS score in the ERAS group was better than that in the traditional group (P ＜ 0.05). The percentage of patients with good prognosis at 1, 6 months after operation in ERAS group was higher than that in the traditional group (P ＜ 0.05). At 1, 6 months after operation, the scores of ADL and FMA in the two groups were higher than those at admission (P ＜ 0.05), which in ERAS group were higher than those in traditional group (P ＜ 0.05). The time of patients staying in hospital in ERAS group was significantly shorter than that in traditional group (P ＜ 0.05). The incidence of re-bleeding, cerebral infarction, mental disorder, urinary tract infection and bedsore after operation in ERAS group were significantly lower than those in the traditional group, and the differences were statistically significant (P ＜ 0.05). There was no significant difference in the incidence of intracranial infection, epilepsy and lung infection between the two groups (P ＞ 0.05). Conclusion ERAS is safe and effective in the patients with hypertensive intracerebral hemorrhage during perioperative period, and it does not increase the risk of complications. ERAS is beneficial to postoperative rehabilitation and shorten the time of hospitalization.
		2019 Vol. 16 (8): 68-71 [Abstract] ( 220 ) HTML (1 KB) PDF (397 KB) ( 145 )

	72	Changes of immune cell function in children with EB virus infection and its clinical significance
		LIU Xuekai1 WANG Yingshi1 XIN Qin1 GAO Yuhan2
		Objective To study the changes of lymphocyte subsets and T lymphocyte function in peripheral blood caused by Epstein-Barr virus (EBV) infection in children, and to explore the relationship between EBV DNA load and lymphocyte subsets. Methods Twenty-five children with EBV infection were selected as the infection group from January 2015 to July 2018 in the Aerospace Center Hospital (“our hospital” for short), and another 25 healthy subjects who underwent physical examination in our hospital in the same time were selected as the control group. Flow cytometry was used to detect the expression of perforin and granulase B in peripheral blood lymphocyte subsets and CD8+ T lymphocyte. The levels of IFN-γ and interleukin 4 (IL-4) secreted by peripheral blood mononuclear cells (PBMC) were measured by enzyme-linked immunosorbent assay (ELISA). Serum EBV DNA load was detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR). Results The ratios of CD3+ and CD8+ cells in peripheral blood of the infected group were significantly higher than that of the control group (P < 0.001). The ratio of CD4+ cells was significantly lower than that of the control group (P < 0.001), but there was no significant difference between the absolute values of the two groups(P > 0.05), the ratio of CD4/CD8 in the infection grous was significantly lower than the control group (P < 0.001), but there was no linear correlation between the change in the proportion and serum EBV DNA load (r = 0.19, P > 0.05). The ratio and absolute value of CD19+ cells were lower than those of the control group (P < 0.001). There was no significant difference in the ratio and absolute value of CD16+CD56+ NK cells between the two groups (P > 0.05). Compared with the control group, the secretion of IFN-γ was increased in the infection group (P < 0.01), and the secretion of IL-4 was decreased (P < 0.05). The secretion of perforin and granzyme B in CD8+ T cells were significantly increased (P < 0.05). Conclusion The lymphocyte subsets and their functions in the peripheral blood of the infected group have obvious changes. It is important to detect the changes of lymphocyte subsets for understanding the cellular immune function of children and guiding clinical treatment.
		2019 Vol. 16 (8): 72-75,95 [Abstract] ( 221 ) HTML (1 KB) PDF (865 KB) ( 84 )

	76	Correlation analysis of the expression of plasma miRNA-126 and hypertensive disorder complicating pregnancy
		HE Xiaoyan1 PENG Shihan1 ZHAO Lei1 LUO Yuping1 LIU Xia2
		Objective To study the relationship between the expression of plasma miRNA-126 and hypertensive disorder complicating pregnancy. Methods A total of 93 patients with hypertensive disorder complicating pregnancy admitted in Luzhou People′s Hospital of Sichuan Province from February 2015 to February 2018 were selected as the study subjects and grouped according to their diseases. There were 30 cases in pregnancy hypertension group, 29 cases in mild preeclampsia group and 34 cases in severe preeclampsia group. Another 20 healthy pregnant women who underwent physical examination during the same period were taken as control group. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of plasma miRNA-126 of each group, and the levels of systolic blood pressure, diastolic blood pressure and 24-hour proteinuria level were compared, and the correlation between them and the expression of plasma miRNA-126 were analyzed. Results Compared with the control group, the expression of plasma miRNA-126 was down-regulated in the pregnancy hypertension group, while the expression of plasma miRNA-126 in the mild preeclampsia group was lower than that in the pregnancy hypertension group, and the miRNA-126 expression in the severe preeclampsia group was lower than that in the mild preeclampsia group, the difference were statistically significant (P < 0.05). The systolic blood pressure and 24-hour proteinuria level in pregnancy hypertension group were significantly higher than those in control group; the systolic blood pressure and 24-hour proteinuria level in mild preeclampsia group were higher than those in pregnancy hypertension group; the systolic blood pressure and 24-hour proteinuria level in severe preeclampsia group were higher than those in mild preeclampsia group; the diastolic blood pressure levels in control group were lower than those in pregnancy hypertension group, mild preeclampsia group, severe preeclampsia group, the differences were statistically significant (all P < 0.05). According to Spearman correlation analysis, the expression of miRNA-126 gene in patients with hypertensive disorder complicating pregnancy was negatively correlated with systolic blood pressure, diastolic blood pressure and 24-hour proteinuria level (r = -0.583, -0.626, -0.611, P < 0.05). Conclusion The expression of plasma microRNA-126 in patients with hypertensive disorder complicating pregnancy is significantly down-regulated, and with the aggravation of the disease, the expression of plasma miRNA-126 is down-regulated more obviously. The down-regulation of the expression of plasma miRNA-126 may play an important role in the occurrence and development of hypertensive disorder complicating pregnancy.
		2019 Vol. 16 (8): 76-79 [Abstract] ( 196 ) HTML (1 KB) PDF (612 KB) ( 89 )

	80	Detection of BDNF and CA125 levels in patients with ovarian endometriosis and analysis of their diagnostic value
		LI Hongxia MENG Xianning▲ HUO Zhiping WU Ya′nan WANG Hongli HAO Yuanyuan
		Objective To investigate the changes of brain-derived neurotrophic factor (BDNF) and carbohydrate antigen 125 (CA125) levels in patients with ovarian endometriosis and their diagnostic value. Methods From February 2017 to June 2018, 78 patients with ovarian endometriosis (EMs) admitted to the Department of Obstetrics and Gynecology, China National Petroleum Corporation Central Hospital ("our hospital" for short) were selected as the EMs group. A total of 50 healthy women who underwent routine physical examination in our hospital during the same period were selected as the control group. The expression of BDNF and serum CA125 levels in two groups of patients and the patients with different r-AFS pathological stages were compared. The correlations between the onset and stage of ovarian EMs and the expression of BDNF and the level of serum CA125 were analyzed. The diagnostic efficacy of BDNF, CA125 and combined detection in the diagnosis of ovarian EMs were calculated. Results The expression of BDNF and serum CA125 in patients with EMs were significantly higher than those in the control group (P < 0.05). There were significant differences in BDNF expression and serum CA125 levels between patients with different r-AFS pathological stages (P < 0.05). BDNF expression and serum CA125 levels were significantly increased with the increase of r-AFS pathological stage (P < 0.05). BDNF expression and serum CA125 levels were positively correlated with ovarian EMs and r-AFS pathological stage (P < 0.05). The sensitivity, specificity and accuracy of combined detection of BDNF and CA125 in diagnosis of ovarian EMs were 98.72%, 100.00% and 99.22% respectively, which were significantly higher than those of single detection of BDNF and CA125 (r > 0.7, P < 0.05). Conclusion The abnormal increase of BDNF expression and serum CA125 level are closely related to the occurrence and development of ovarian EMs. The combined detection of BDNF and CA125 level is helpful to the staging diagnosis and evaluation of ovarian EMs.
		2019 Vol. 16 (8): 80-83 [Abstract] ( 183 ) HTML (1 KB) PDF (660 KB) ( 88 )

	84	Clinical effect of Diphereline combined with laparoscopy surgery in the treatment of endometriosis
		XU Chang CHEN Ying LU Yulian
		Objective To investigate the clinical effect of Diphereline combined with laparoscopy surgery in the treatment of endometriosis. Methods A total of 103 patients with endometriosis admitted to the First People′s Hospital of Yongkang City, Zhejiang Province were selected from January 2016 to October 2017, and they were divided into control group(51 cases) and observation group(52 cases) according to random table method. The control group was treated with laparoscopic surgery, the observation group was treated with Diphereline combined with laparoscopic surgery. The effective rate, pelvic mass size, quality of life score for endometriosis (EHP-5), dysmenorrhea visual analogue scale (VAS), sex hormone level and vascular related indices were evaluated and compared between the two groups before and after treatment. The incidence of adverse reactions was recorded and compared between the two groups. Results The total effective rate of the observation group was higher than the control group (P ＜ 0.05). After treatment, the pelvic mass size, EHP-5 score and dysmenorrhea VAS score in the two groups were lower than those before treatment, and those in the observation group were lower than those in the control group, the differences were statistically significant (P ＜ 0.05). After treatment, the levels of luteinizing hormone (LH), estradiol (E2), follicle-stimulating hormone (FSH), vascular growth factor(VEGF), Angiopoietin (Ang)-2 and Angiopoietin receptor (Tie)-2 in the two groups were lower than those before treatment, and those in the observation group were lower than those in the control group, the differences were statistically significant (P ＜ 0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P ＞ 0.05). Conclusion The clinical efficacy of Diphereline combined with laparoscopy surgery in the treatment of endometriosis is remarkable, it can significantly reduce the body′s sex hormone level, VEGF, Ang and other related factors expression level, and improve the clinical treatment effect of endometriosis, which can provide some ideas for clinical research.
		2019 Vol. 16 (8): 84-87 [Abstract] ( 210 ) HTML (1 KB) PDF (660 KB) ( 88 )

	88	Clinical effect of high frequency oscillatory ventilation combined with Sildenafil in the treatment of neonatal persistent pulmonary hypertension
		WU Yanqiu1 XU Dongmei1 ZHOU Qili1 WANG Zhijie1 LIU Huiling2 DONG Zhiyong3 LIU Xia1
		Objective To investigate the effect of high frequency oscillatory ventilation combined with Sildenafil in the treatment of neonatal persistent pulmonary hypertension (PPHN). Methods A total of 109 newborns with PPHN admitted to the Affiliated Hospital of Chengde Medical College from March 2014 to March 2018 were selected as subjects. Newborns were divided into control group (54 cases) and study group (55 cases) according to random number table method. The control group was treated with high frequency oscillatory ventilation, and the study group was treated with Sildenafil. The clinical effect of the two groups was compared after 72 hours′ treatment. The arterial partial pressure of carbon dioxide (PaCO2), fraction of inspired oxygen (FiO2), arterial oxygen saturation (SaO2), arterial partial pressure of oxygen (PaO2), pulmonary artery pressure (SPAP) and systemic blood pressure (SBP) were compared between the two groups before and after treatment. Adverse reactions of the two groups during treatment were observed. Results The total effective rate of the study group was significantly higher than that of the control group, and the difference was statistically significant (P ＜ 0.05). The PaCO2 and FiO2 of the two groups were lower than those before treatment, and those in the study group were lower than those in the control group (P ＜ 0.05). The SaO2 and PaO2 of the two groups increased after treatment, and those in the study group were higher than those in the control group (P ＜ 0.05). The SPAP of the study group after treatment was significantly lower than that before treatment (P ＜ 0.05), while there was no significant difference in the control group before and after treatment (P ＞ 0.05), and that in the study group was lower than the control group (P ＜ 0.05). There was no significant difference in SBP before and after treatment in the same group nor between the two groups (P ＞ 0.05). There was no significant difference in the incidence of adverse reactions between the two groups during treatment (P ＞ 0.05). Conclusion High frequency oscillatory ventilation combined with Sildenafil in the treatment of PPHN is effective remarkbly, which can significantly improve clinical symptom and related clinical indicators, and it has high clinical application value.
		2019 Vol. 16 (8): 88-91 [Abstract] ( 266 ) HTML (1 KB) PDF (613 KB) ( 138 )

	92	Study on the current situation and risk factors of infection in Department of Neonatology in Affiliated Hospital of North Sichuan Medical College
		LIU Jiaojiao1 FANG Ping2▲
		Objective To explore the situation and risk factors of hospital infection in Department of Neonatology， Affiliated Hospital of North Sichuan Medical College (“our hospital” for short) and clarify the direction of intervention. Methods A cross-sectional study was conducted on newborns hospitalized in our hospital′s Department of Neonatology from January to December 2017. Researches were carried on for 12 times during the first week of each month， and 592 hospitalized children were enrolled. Univariate and multivariate Logistic regression analysis were used to analyze and screen the risk factors of nosocomial infection in neonatology. Results In 2017， there were 21 children with nosocomial infection totally in the Department of Neonatology， with an incidence of 3.53% (21/592)， and no statistical differences were found in the infection rate among quarters or gender (P ＞ 0.05). High birth weight (OR = 0.221) and full term gestational age (OR = 0.081) were protective factors for nosocomial infection of newborn (P ＜ 0.05). Prolong of hospitalization (OR = 6.023)， intravenous nutrition (OR = 2.290)， respiratory distress syndrome (OR = 1.688)， neonatal pneumonia (OR = 1.876) and neonatal hyperbilirubinemia (OR = 1.672) were risk factors for neonatal nosocomial infection (P ＜ 0.05). Conclusion Low gestational age， low birth weight， long hospitalization and neonatal related diseases are the main risk factors for nosocomial infection in Department of Neonatology. In order to reduce the incidence of nosocomial infection the hospital should strengthen training on nosocomial infection， implement disinfection and isolation measures and so on.
		2019 Vol. 16 (8): 92-95 [Abstract] ( 263 ) HTML (1 KB) PDF (662 KB) ( 75 )

	96	Application value of colposcope biopsy and with loop electrosurgical excision procedure in the diagnosis and treatment of cervical lesions
		LIU Ming
		Objective To explore the value of cervical biopsy combined loop electrosurgical excision procedure (LEEP) in the diagnosis and treatment of cervical lesions. Methods Clinical data of 159 patients diagnosed by cervical biopsy under colposcope because of abnormal cervical TCT and/or HPV detection and volunteered for cervical ring electrotomy (LEEP) in Affiliated Hospital of Qingdao University from January 2016 to May 2017 were retrospectively analyzed, the results of and pathological examination after LEEP operation and diagnose accordance rate were observed. Meanwhile, the efficacy of LEEP in patients with cervical lesions was observed. Results Among 159 patients, there were 2, 4, 60, 59 patients were diagnosed as chronic cervicitis, condyloma, cervical intraepithelial neoplasia (CIN) Ⅰ, CIN Ⅱ/CIN Ⅲ both by cervical biopsy under colposcope and pathological examination after LEEP surgery respectively, 4 patients were diagnosed as cervical carcinoma in situ by pathological examination after LEEP surgery, and the coincidence rate was 78.62%. Among 4 cases of chronic cervicitis, there were 2 cases were pathologically up to CIN Ⅰ; among 11 patients with condyloma, 5 patients were pathologically up to CIN Ⅰ, 2 cases pathologically up to the CIN Ⅱ/CIN Ⅲ; among 76 patients of CIN Ⅰ, 11 cases were pathologically up to CIN Ⅱ/CIN Ⅲ, 5 cases down to cervicitis; among 68 patients of CIN Ⅱ/CIN Ⅲ, 2 cases were pathologically up to cervical carcinoma in situ, 7 cases were pathologically down to CIN Ⅰ. The consistency in the diagnosis of cervical lesions between cervical biopsy and LEEP was 0.632, with a high consistency. All patients successfully completed LEEP surgery, with an average operative time (27.50±5.03) min and an average intraoperative blood loss (31.75±8.29) mL. Except for 4 cases of cervical cancer patients diagnosed by LEEP postoperative pathological, 155 patients were all implement regular follow-up, follow-up time was 12-28 months, TCT examination results 6 months after operation showed that 155 patients without CIN, the cure rate was 97.48%, 2 patients were diagnosed as CIN Ⅱ again 1 year after operation. Conclusion Cervical biopsy under colposcope and histopathological examination after LEEP surgery are effective methods for the diagnosis of CIN and early cervical cancer, with a high consistency of two methods. The LEEP surgery can further lower the missed diagnosis rate of the high grade of CIN and cervical cancer, which is worthy of clinical application.
		2019 Vol. 16 (8): 96-99 [Abstract] ( 167 ) HTML (1 KB) PDF (655 KB) ( 58 )

	100	Efficacy of OxyContin combined with Zoledronic Acid in treatment of advanced breast cancer bone metastasis cancer pain concurrent with chemoradiation therapy
		WU Kuo LIU Mengqi ZHAO Qingshu
		Objective To observe the clinical efficacy of OxyContin combined with Zoledronic Acid in the treatment of advanced breast cancer with bone metastasis cancer pain concurrent with chemoradiation therapy. Methods From April 2017 to April 2018, in Jizhong Energy Xingtai Mining Group General Hospital, 80 patients with advanced breast cancer bone metastasis cancer pain concurrent with chemoradiation therapy were selected, according to random number table method, they were divided into control group and observation group, with 40 cases in each group. Patients in the control group were treated with OxyContin for analgesia, patients in the observation group were treated with OxyContin and Zoledronic Acid for analgesia. The pain level was evaluated by numeric rating scale (NRS), the quality of life of the two groups was evaluated by Karnofsky functional status score (KPS) and the quality of life score (QOL) of the tumor patients, the increase and decrease of OxyContin dosage and adverse reactions in the two groups were observed. Results After 28 days of treatment, the NRS scores of patients in the two groups were lower than before treatment, the KPS score and QOL score were higher than before treatment, the differences were statistically significant (P < 0.05). The NRS scores of patients in the observation group were lower than the control group, the KPS score and QOL score were higher than the control group, the differences were statistically significant (P < 0.05). The rate of OxyContin reduction of patients in the observation group was higher than control group, the difference was statistically significant (P < 0.05). The incidence of constipation of patients in the observation group was lower than control group, the difference was statistically significant (P < 0.05). Conclusion OxyContin combined with Zoledronic Acid has a definite effect in the treatment of advanced breast cancer bone metastasis cancer pain concurrent with chemoradiation therapy. It can effectively reduce the degree of cancer pain, improve the quality of life of patients, and reduce the adverse effects of the simple application of OxyContin, worthy of clinical promotion.
		2019 Vol. 16 (8): 100-103 [Abstract] ( 195 ) HTML (1 KB) PDF (546 KB) ( 62 )

	104	Effect of different doses of Aspirin combined with chemotherapy on prognosis of patients with advanced colorectal cancer
		ZHU Zhou QIN Jianing CHEN Rixin LI Jingchang LI Min GUO Xufei LIN Cheng TAN Zhenzhe NI Bingqiang
		Objective To investigate the effect of different doses of Aspirin combined with chemotherapy on the prognosis of patients with advanced colorectal cancer. Methods From January 2012 to April 2013, in Liuzhou People′s Hospital, 80 patients with advanced colorectal cancer were selected, they were divided into low dose group (40 cases) and high dose group (40 cases), low dose group was given Aspirin 100 mg/d after chemotherapy, and high dose group was given Aspirin 200 mg/d after chemotherapy. The total effective rate of the two groups after treatment was compared, the progression-free survival time (PFS), 3-year overall survival rate (OS) and 5-year OS of the two groups was recorded. The serum tumor marker before and after treatment was compared, and the adverse reaction was recorded. Results Total effective rate and disease control rate in high dose group were significantly higher than low dose group, the differences were statistically significant (P < 0.05). After treatment, serum CA199 and CEA of two groups were lower than before treatment, and these of the high dose group were lower than low dose group, the differences were statistically significant (P < 0.05). PFS, 3-year OS and 5-year OS in high dose group were significantly higher than low dose group, the differences were statistically significant (P < 0.05). The total incidence of adverse reactions in high dose group was higher than low dose group, the difference was statistically significant (P < 0.05). Conclusion High dose aspirin therapy for advanced colorectal cancer patients after chemotherapy has a better curative effect, it can significantly reduce the level of serum tumor markers, improve the survival rate of patients, but there are many adverse reactions, clinical patients can choose the dose according to the specific circumstances.
		2019 Vol. 16 (8): 104-107 [Abstract] ( 192 ) HTML (1 KB) PDF (499 KB) ( 79 )

	108	Significance of mismatch repair/microsatellite instability in the chemotherapy sensitivity and prognosis of late-stage colorectal cancer
		WANG Li MAO Haiyan TONG Jiandong WANG Zhu▲
		Objective To investigate the significance of mismatch repair/microsatellite instability (MSI) in the chemotherapy sensitivity and prognosis of late-stage colorectal cancer (CRC). Methods From December 2009 to December 2016, in Affiliated Hospital of Yangzhou University, 181 primary tumor tissues were collected from CRC patients who received first-line chemotherapy (FOLFOX or XELOX). Immunohistochemistry was used to detect the expression of MLH1, PMS2, MSH2 and MSH6 in tumor tissues. Based on the staining results, they were divided into MSI group (18 cases) and microsatellite stability (MSS) group (163 cases). The intergroup difference in chemotherapy sensitivity and prognosis were analyzed. Results Except for tumor location (P < 0.05), the differences were not statistically significant in clinical features between two groups (P > 0.05). Kaplan-Meier model was used to analyze follow-up data, there was no statistically significant difference in patient prognosis of two groups (P > 0.05). In chemotherapy sensitivity, there was no statistically significant difference in the total effective rate of two groups (P > 0.05). However, disease control rate of MSI group was significant higher than MSS group, the difference was statistically significant (P < 0.05). Conclusion Although MSI status is not related with the prognosis of late-stage CRC patients, but it is correlated with disease control rate. Therefore, MSI can act as an important marker for evaluating the chemotherapy efficacy in late-stage CRC patients.
		2019 Vol. 16 (8): 108-111,127 [Abstract] ( 249 ) HTML (1 KB) PDF (983 KB) ( 98 )

	112	Effect of Brucea Javanica Oil Emulsion combined with GP chemotherapy on curative effect and immune function of patients with non-small cell lung cancer
		DENG Lan1 PENG Guolin1 JIANG Mo1 JIANG Yilan2
		Objective To study the effect of Brucea Javanica Oil Emulsion combined with Cisplatin (GP) chemotherapy on the curative effect and immune function of patients with non-small cell lung cancer (NSCLC). Methods From January 2017 to January 2018, in the 184th Hospital of PLA and Affiliated Hospital of Institute of Traditional Chinese Medicine of Hu'nan Province, 80 patients with NSCLC were treated with chemotherapy were selected, they were divided into observation group and control group by random grouping method, with 40 cases in each group. The control group was treated with GP chemotherapy, and the observation group was treated with Brucea Javanica Oil Emulsion on the basis of control group. The clinical effect was compared between the two groups, the immune function index (CD3+, CD4+, CD8+, CD4+/CD8+) and quality of life in the two groups before and after treatment were compared, the adverse reaction of the two groups during the treatment was observed. Results After treatment, the total effective rate of the observation group was significantly higher than the control group, the difference was statistically significant (P < 0.05). Before treatment, there was no statistically significant difference in immune function before treatment (P > 0.05), after treatment, the positive rate of CD3+ and CD4+ cells and the ratio of CD4+/CD8+ in the observation group were higher than the control group, while the positive rate of CD8+ cells was lower than the control group, the differences were statistically significant (P < 0.05). Before treatment, there was no statistically significant difference in KPS score of the two groups (P > 0.05), after treatment, the KPS score of the two groups was increased significantly, and this of the observation group was higher than the control group, the differences were statistically significant (P < 0.05). The incidence of adverse reaction of the observation group was significantly lower than that in the control group, the difference was statistically significant (P < 0.05). Conclusion Brucea Javanica Oil Emulsion combined with GP chemotherapy in the treatment of NSCLC patients has a significant short-term effect, it can improve the immune function, reduce the incidence of adverse reactions, and improve the life quality of the patients, and provide a new reference for the chemotherapy of non-small cell lung cancer.
		2019 Vol. 16 (8): 112-115 [Abstract] ( 231 ) HTML (1 KB) PDF (537 KB) ( 52 )

	116	Serum adipocyte-type fatty acid binding protein and interleukin-17 levels in patients with type 2 diabetes mellitus and their relationship with lower limb atherosclerosis
		WANG Lu1 BAI Hua1 HAN Mei1 ZHAO Yuyan2
		Objective To investigate the relationship between serum adipocyte-type fatty acid binding protein (A-FABP) and interleukin-17 (IL-17) expression levels and lower limb atherosclerosis (LEAD) in patients with type 2 diabetes. Methods A total of 131 patients with type 2 diabetes admitted to Jinqiu Hospital of Liaoning Province (hereinafter referred to as "our hospital") from February 2017 to November 2017 were divided into non-lead group (n = 34) and non-lead group (n = 97) according to ankle-brakle index (ABI), and 45 healthy subjects in our hospital were randomly selected as the control group during the same period. Serum levels of A-FABP and IL-17 were determined by enzyme-linked immunosorbent assay, and the influencing factors of LEAD in type 2 diabetes were analyzed by Logistic regression. ROC curve was used to evaluate the predictive value of A-FABP and IL-17 on LEAD. Results The duration of diabetes in the LEAD group was longer than that in the non-lead group (P ＜ 0.05). The systolic blood pressure, glycosylated hemoglobin (HbA1c), fasting blood glucose (FBG), low-density lipoprotein cholesterol (LDL-C), serum creatinine (SCr), A-FABP, and IL-17 levels in the LEAD group and the non-lead group were higher than those in the control group (P ＜ 0.05). Multivariate Logistic regression analysis showed that LDL-C, A-FABP and IL-17 were independent risk factors of LEAD in type 2 diabetes (OR = 1.418, 1.657, 1.619, P ＜ 0.05). The area under the curve (AUC) of A-FABP combined with IL-17 was 0.906, the sensitivity and specificity were 0.89 and 0.86, respectively, and the accuracy was 0.90. Conclusion Serum A-FABP and IL-17 are independent risk factors of LEAD in patients with type 2 diabetes. Early combined detection is helpful to predict LEAD in the early clinical stage, so as to guide clinical development of early intervention measures.
		2019 Vol. 16 (8): 116-119,135 [Abstract] ( 160 ) HTML (1 KB) PDF (760 KB) ( 51 )

	120	Meta analysis of the effect of laser treatment on lumbago
		LI Hetong1 GOU Yu2 ZHANG Liu1,3▲
		Objective To investigate the clinical efficacy of laser treatment of low back pain (LBP), and Meta analgsis was porformed. Methods Domestic and foreign databases, such as PubMed, Embase, the Cochrane Library, CNKI, WIP and Wanfang, were searched by computer, and the eligible literatures were screened, and divided into experimental group and control group according to intervention weasures, and their methodological quality was evaluated. Meta analysis was conducted on the included literatures through RevMan 5.3 software. Results Finally, 14 randomized controlled trials were included, with a total of 1117 patients. Meta analysis results showed that visual simulation score (VAS) in the laser group was better than that in the control group [MD = -0.77; 95%CI (-1.04, -0.50); P < 0.000 01]. The improvement score of the Roland-Morris dysfunction questionnaire (RDQ) in the laser group was better than that in the control group [MD = -1.37; 95%CI (-1.97, -0.76); P < 0.000 01]. The modified oswestry disability questionnaire (MODQ) score in the laser group was superior to that in the control group [MD = -2.69; 95%CI (-4.45, -0.94); P = 0.003]. Conclusion Laser therapy has certain curative effect and advantage in improving the pain symptoms and lumbar activities of patients with low back pain.
		2019 Vol. 16 (8): 120-123,135 [Abstract] ( 216 ) HTML (1 KB) PDF (1177 KB) ( 70 )

	124	Effect of 5-aminolevulinic acid photodynamic therapy combined with imiquimod in the treatment of HIV/AIDS with perianal condyloma acuminatum
		WANG Jing YANG Xiaolin WANG Zhangli ZHANG Hongwei DAI Lili SHAO Ying▲
		Objective To observe the clinical effect of 5-aminolevulinic acid photodynamic (ALA-PDT) combined with imiquimod in the treatment of perianal condyloma acuminatum. Methods The clinical data of 146 patients with HIV/AIDS and perianal condyloma acuminata treated in Beijing You′an Hospital from February 2016 to October 2017 were analyzed. According to different treatments, they were divided into observation group and control group, 82 patients in the observation group were treated with 5-aminolevulinic acid photodynamic therapy （ALA-PDT） combined with Imiquimod treatment, 64 patients in the control group were treated with ALA-PDT combined with laser, and the clinical efficacy and adverse reaction rate between the two groups were compared. Results After 6 months of treatment, the total effective rate of the observation group was significantly higher than that of the control group, the difference was statistically significant (P ＜ 0.05). The incidence of patients with pain discomfort and local erosion in the observation group was significantly lower than that of the control group, the differences were statistically significant (P ＜ 0.05). Conclusion ALA-PDT combined with imiquimod has significant curative effect and low adverse reaction rate in the treatment of perianal condyloma acuminatum. It is worth further promotion in clinical practice.
		2019 Vol. 16 (8): 124-127 [Abstract] ( 185 ) HTML (1 KB) PDF (596 KB) ( 80 )

	128	Analysis of risk factors for hyperuricemia and gout
		ZHU Gang1,2 HUANG Chuanbing2▲ LIU Sidi1,2 SHI Zhouzhen1,2 WANG Guizhen2 WAN Lei2
		Objective To explore and analyze the correlation between high risk factors of hyperuricemia and gout. Methods From April 2015 to April 2017, 90 cases of hyperuricemia patients and 90 cases of gout patients admitted to the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine were selected as the hyperuricemia group and the gout group respectively, Logistic multivariate regression model was used to analyze the correlation between the occurrence of gout and obesity, liver function, blood lipid and other factors. Results Compared with the high uric acid group, the gout group had a higher proportion of male patients, higher proportion of patients with total cholesterol ≥5.72 mmol/L and triglycerin (TG) ≥1.71 mmol/L, and higher proportion of patients with high density lipoprotein cholesterol ＜0.91 mmol/L. The proportion of patients with low-density lipoprotein cholesterol(LDL-C) ≥3.37 mmol/L, apolipoprotein B ≥1.20 g/L and alanine aminotransferase ≥50 U/L was higher, and the difference were statistically significant (P ＜ 0.05). There was no significant difference in BMI and apolipoprotein A levels between the two groups (P ＞ 0.05). The above factors were analyzed by Logistic multivariate regression, and it was found that male, TG ≥1.71 mmol/L, and LDL-C≥3.37 mmol/L could be considered as high risk factors for gout (OR = 2.05, 2.45, 3.12, respectively). Conclusion Men can be the main morbidity group of gout, and high TG and LDL-C can be the high risk factors of gout.
		2019 Vol. 16 (8): 128-130 [Abstract] ( 202 ) HTML (1 KB) PDF (603 KB) ( 107 )

	131	Correlation between coronary artery lesion score and serum cystatin C level in acute ST-segment elevation myocardial infarction patients
		ZHANG Shiyin1 LIAO Haihong2 LI Guodong1
		Objective To investigate the correlation between coronary artery lesion score and serum cystatin C (CysC) level in acute ST-segment elevation myocardial infarction (STEMI). Methods A total of 384 STEMI patients admitted to Huzhou First People′s Hospital of Zhejiang Provmce from September 2016 to April 2018 were selected. The CysC level was recorded. Patients were assessed for coronary artery disease by coronary artery stenosis score (Gensini). The correlation between CysC level and Gensini score was observed. Results The CysC levels gradually decreased with the increase of lesion severity, and there was remarkably statistically significant difference among the groups (P ＜ 0.01). The Pearson correlation analysis showed that gender, residence, alcohol consumption were not correlated with the Gensini score of STEMI patients (P ＞ 0.05), and the gensini score was positively correlated with onset to intervening time, age, diabetes, hypertension, hyperlipidemia, smoking, family history of coronary heart disease, body mass index, blood glucose, total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), systolic blood pressure, diastolic blood pressure, creatine kinase isoenzyme (CK-MB), brain natriuretic peptide (NT-proBNP), hypersensitive c-reactive protein (hs-CRP) (P ＜ 0.05), while the CysC, high density lipoprotein cholesterol (HDL-C), left ventricular ejection fraction (LVEF) were negatively correlated with Gensini score (P ＜ 0.05). Gensini score was used as dependent variable, other factors as independent variables were analyzed in multiple regression analysis, and the results showed that LDL-C, CysC, hs-CRP were independent risk factors affecting Gensini evaluation (P ＜ 0.05). Conclusion The CysC level in acute STEMI patients are negatively correlated with Gensini levels, which is helpful to assess the severity of STEMI.
		2019 Vol. 16 (8): 131-135 [Abstract] ( 211 ) HTML (1 KB) PDF (625 KB) ( 111 )

	136	Prognostic value of CK-MB, cTnI, BNP, BUN and cystatin in patients with carbon monoxide poisoning
		JIN Lili1 QIE Tao2 ZHANG Tongle1 LI Liqin3 LIANG Qing2 TIAN Xiang4
		Objective To explore the prognostic value of creatine kinase isozyme (CK-MB), serum troponin (cTnI), brain natriuretic peptide (BNP), urea nitrogen (BUN), cystatin in patients with carbon monoxide poisoning. Methods From October 2016 to March 2018, 136 patients with acute carbon monoxide poisoning treated in the First Central Hospital of Baoding City were selected as the study subjects. According to the clinical prognosis, the patients were divided into good prognosis group (n = 122) and poor prognosis group (n = 14). The levels of CK-MB, cTnI, BNP, BUN and cystatin were compared and analyzed between the two groups at admission and 7 days after admission. Results The levels of CK-MB, BNP and cystatin were significantly lower in the good prognosis group than in the poor prognosis group, and the differences were highly statistically significant (P < 0.01). The levels of CK-MB, cTnI, BNP, BUN and cystatin in the good prognosis group and the poor prognosis group were significantly lower than those at admission, and the differences were statistically significant (P < 0.05). The levels of cTnI, BNP and cystatin in the good prognosis group were significantly lower than those in the poor prognosis group 7 days after admission, and the differences were highly statistically significant (P < 0.01). The correlations between BNP levels at admission and 7 days after admission and the prognosis of carbon monoxide poisoning were good. The areas under the curve were 0.861 and 0.856, respectively, which were much higher than the area under the curve of other indicators. The specificity and sensitivity of BNP at admission were 71.4% and 91.8%, respectively. The specificity and sensitivity of BNP 7 days after admission for were 64.3% and 97.5%, respectively, which were higher than other indicators. Conclusion The level of BNP in patients with carbon monoxide poisoning at admission and after treatment has a good correlation with the prognosis of carbon monoxide poisoning. It has good specificity and sensitivity, and can be used as an effective marker to judge the prognosis of patients with carbon monoxide poisoning.
		2019 Vol. 16 (8): 136-139 [Abstract] ( 200 ) HTML (1 KB) PDF (655 KB) ( 142 )

	140	Quality of life in children and adolescents with type 1 diabetes mellitus in Qinhuangdao
		QUAN Liyuan LI Mingli XIA Mingyue FENG Yue HUANG Wei LIU Ling
		Objective To explore the influencing factors of quality of life of children and adolescents with type 1 diabetes mellitus in Qinhuangdao area. Methods A total of 110 children and adolescents with type 1 diabetes who were enrolled in the First Hospital of Qinhuangdao and county-level hospital in Qinhuangdao from January 2015 to June 2018 were selected as observation group, while 110 healthy children and adolescents were selected as control group. The qualities of life of the two groups were assessed by the Pediatric Quality of Life Inventory Measurement Models. Results The scores of physiological function, emotional function, social function, school function and total score of the observation group were significantly lower than those of the control group, and the differences were statistically significant (P < 0.05). The rates of poor quality of life in parents separated or divorced, mother junior high school culture below, father junior high school culture below, and non-only children were significantly higher than those of parents who were married or cohabited, mother junior high school culture above, father junior middle school culture above, and only one children (P < 0.05). Logistic regression analysis showed that parents′ separation or divorce and non-only children were risk factors for the quality of life of children and adolescents with type 1 diabetes mellitus (OR = 2.489, 1.988, P < 0.05), and the mother′s educational level in junior middle school and above was the protective factor for the quality of life of children and adolescents with type 1 diabetes mellitus (OR = 0.800, P < 0.05). Conclusion The quality of life of children and adolescents with type 1 diabetes in Qinhuangdao area are related to their parents′marital status, whether they have only one child or not and their mother′s educational level.
		2019 Vol. 16 (8): 140-142,147 [Abstract] ( 199 ) HTML (1 KB) PDF (971 KB) ( 124 )

	143	Meta analysis on the effect comparison of Nebivolol and the second generation of beta blockers in the treatment of hypertension
		ZHAO Qun
		Objective To compare the effect of Nebivolol and the second generation of beta blockers in the treatment of hypertension by Meta analysis. Methods The databases of PubMed, Cochrane Library, EMbase, China National Knowledge Infrastructure (CNKI), Chinese biomedicine literature database (CBM), WanFang data were electronically searched from inception to December 2015. The case-control studies about Nebivolol and the second generation of beta blockers such as Metoprolol in the treatment of hypertension were screened, and the data of changes of blood pressure, heart rate after administration and adverse reactions was obtained, then the Meta-analysis was performed by RevMan 5.3 software. Results Seven studies were enrolled including 481 cases with hypertension. After treatment, there was no statistically significant difference of systolic pressure [MD = 0.18，95%CI (-1.50, 1.86)，P = 0.83], while there were statistically significant differences of diastolic pressure [MD = 1.07，95%CI (0.24, 1.90), P = 0.01], heart rate [MD = -4.20, 95%CI (-5.19, -3.20), P < 0.00001], and there was no statistically significant difference of adverse reactions [MD = 0.52, 95%CI (0.27, 0.99), P = 0.05] between Nebivolol and the second generation of beta blockers in the treatment of patients with hypertension. Conclusion The falling range of diastolic blood pressure treated by Nebivolol in patients with hypertension is bigger than the second generation of beta blockers, the change of heart rate is less, the security of Nebivolol and the second generation of beta blockers is almost the same.
		2019 Vol. 16 (8): 143-147 [Abstract] ( 199 ) HTML (1 KB) PDF (1273 KB) ( 74 )

	148	Effect of Bifidobacterium Tetravaccine Tablets combined with early enteral nutrition in the treatment of severe craniocerebral injury
		PEI Tingna1 GAO Shujie2 LI Chunmin3
		Objective To investigate the effect of Bifidobacterium Tetravaccine Tablets combined with early enteral nutrition on severe craniocerebral injury. Methods A total of 102 patients with severe craniocerebral injury who were treated in Benxi Central Hospital of Liaoning Province from January 2016 to May 2018 were selected. The patients were randomly divided into control group (51 cases) and study group (51 cases) according to the random number table method. The control group was given early enteral nutrition treatment. The study group was treated with Bifidobacterium Tetravaccine Tablets on the basis of the control group. The nutritional status, humoral immunity and inflammatory factors of the two groups were compared before and after treatment, the complications in the two groups during treatment were recorded. Results After treatment, the levels of albumin, albumin, prealbumin and hemoglobin in the two groups were higher than those before treatment, which of the study group were higher than those of the control group (P ＜ 0.05). After treatment, the levels of immune globulin A, immune globulin G and immune globulin M in the two groups were all higher than those before treatment, which of the study group were higher than those of the control group (P ＜ 0.05). After treatment, the levels of inflammatory factors interleukin-6, C-reactive protein, tumor necrosis factor-α and white blood cell counts in the two groups were all lower than those before treatment, which of the study group were lower than those of the control group (P ＜ 0.05). The incidence of complications during treatment in the study group was significantly lower than that in the control group (P ＜ 0.05). Conclusion Bifidobacterium Tetravaccine Tablets combined with early enteral nutrition in the treament of severe craniocerebral injury can effectively improve the nutritional status of patients, enhance immunity, alleviate inflammatory reactions, and reduce the incidence of complications, which has a certain clinical value.
		2019 Vol. 16 (8): 148-151 [Abstract] ( 177 ) HTML (1 KB) PDF (485 KB) ( 80 )

	152	Clinical study of Yangwei Granules combined with quadruple therapy in the treatment of chronic atrophic gastritis with positive Hp
		ZHANG Lele1 GENG Lei2 LIN Xurong1 DANG Hong1
		Objective To investigate the clinical efficacy of Yangwei Granules combined with quadruple therapy in the treatment of chronic atrophic gastritis (CAG) with positive Helicobacter pylori (Hp). Methods Eighty-one cases of Hp-positive CAG admitted to Fushun Hospital of Traditional Chinese Medicine from January 2015 to January 2017 were selected, they were randomly divided into control group (40 cases) and study group (41 cases) according to the number table method. The control group was treated with quadruple therapy and the study group was treated with Yangwei Granules combined with quadruple therapy.The clinical efficacy and Hp eradication of the two groups was compared at 90 d after treatment. The levels of motilin (MLT), endothelin (ET), gastrin (GAS), pepsinogen Ⅰ (PGI), pepsinogen Ⅱ (PGⅡ), PGⅠ/PGⅡ were recorded before and 90 d after treatment, the adverse reactions during the treatment were recorded. Results 90 days after treatment, the total effective rate in the study group was significantly higher than the control group (P ＜ 0.05). The eradication rate of Hp in the study group was significantly higher than that in the control group (P ＜ 0.05). After 90 days of treatment, MLT and ET in the two groups were lower than those before treatment, and the GAS in the study group was higher than that in the control group (P ＜ 0.05), which in the study group was higher than that in the control group (P ＜ 0.05). There were no significant differences in PGⅡ between the two groups before and 90 days after treatment (P ＞ 0.05); PGⅠ, PGⅠ/PGⅡ in the two groups increased significantly after 90 days of treatment, and the study group was higher than the control group (P ＜ 0.05). There was no significant difference in the incidence of adverse reactions between the two groups during treatment (P ＞ 0.05). Conclusion Yangwei Granules combined with quadruple therapy is effective in the treatment of Hp-positive CAG. It can effectively improve the level of pepsinogen and gastrointestinal hormones without increasing the incidence of adverse reactions. It has high clinical value.
		2019 Vol. 16 (8): 152-155 [Abstract] ( 311 ) HTML (1 KB) PDF (490 KB) ( 72 )

	156	Meta-analysis of the effect of psychological intervention on the quality of life and psychological status of leukemia patients
		LIU Wenfeng DENG Huan FU Xiaofeng WANG Yunyun▲
		Objective To assess the effect of psychological intervention on the quality of life and psychological status of leukemia patients. Methods The relevant literatures home and abroad were retrieved from the database established to August 2018 with the keywords of “leukemia”“psychological intervention”“quality of life”“psychological state”“anxiety and depression” in Chinese and English. Quality of life scale (QLQ-C30), self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were used as outcome indicators, and Meta analysis was conducted by RevMan 5.3 software. Results A total of 23 studies involving 1833 patients were included, with 929 cases in intervention group and 904 cases in control group. After psychological intervention, the total scores of QLQ-C30 (MD = 9.73, 95％CI: 7.51-11.95, P ＜ 0.01), SAS (MD = -8.54, 95％CI: -10.96--6.12, P ＜ 0.01), SDS (MD = -8.88, 95％CI: -11.47--6.29, P ＜ 0.01) in the psychological intervention group were significantly better than those in the control group. Conclusion Psychological intervention could significantly improve the quality of life and relieve the anxiety and depression symptoms of leukemia patients.
		2019 Vol. 16 (8): 156-160 [Abstract] ( 242 ) HTML (1 KB) PDF (1488 KB) ( 92 )

	161	Construction of the evaluation index system of the core competence of palliative care nurse
		LI Xue1 PENG Qing1 SU Jianping1 WANG Juan2 LI Xiaofeng2 WANG Huihui1 MA Lili1
		Objective To construct the core competence evaluation index system of palliative care nurse, and to provide objective reference standard for appointment, training and assessment of palliative care training system. Methods The framework of core competence evaluation index system was established by literature review and expert meeting, and 32 nursing experts were consulted twice by Delphi method, and the weight of each index was determined by analytic hierarchy process. Results The response rates of the two rounds of expert consultation were 100%, and the expert authority coefficient was 0.82-0.95. The coordination coefficients of the first, second and third grade indices were 0.366, 0.374 and 0.320 (P ＜ 0.001), respectively. Finally, the core competence evaluation index system of nursing specialists in palliative care was established, including 3 first-level indicators, 29 second-level indicators and 120 third-level indicators. Conclusion This questionnaire has a high degree of expert initiative and authority, and the coordination of opinions is favorable. The developed core competence evaluation index system can provide objective reference standards for the appointment, training and assessment of palliative care nurse.
		2019 Vol. 16 (8): 161-164 [Abstract] ( 260 ) HTML (1 KB) PDF (554 KB) ( 118 )

	165	Effect of nursing intervention on relieving pain in minimally invasive small incision thumb valgus correction
		WANG Xiaorui1 HOU Shengfang2 ZHANG Yahui3
		Objective To study the effect of nursing intervention on relieving pain in minimally invasive small incision thumb valgus correction. Methods Two hundred patients undertaking minimally invasive small incision thumb valgus correction were selected from January 2016 to May 2018 in Hebei Medical University Third Hospital. According to the random number table method, 200 patients were divided into control group and observation groups, with 100 cases in each group. The routine perioperative nursing intervention was performed in the control group, and comprehensive nursing was performed in the observation group. The functional score of hallux valgus angle, hallux-metatarsophalangeal joint function score, the score of pain, the score of comfort, the score of sleep quality, the score of psychological state, the score of life quality and nursing satisfaction rate were compared between the two groups. Results After nursing, the hallux valgus angle in both groups decended compared with that before nursing (P ＜ 0.05), and the functional score of the hallux valgus and metatarsophalangeal joint in both groups increased compared with that before nursing (P ＜ 0.05). After nursing, the hallux valgus angle of the observation group was smaller than that of the control group (P ＜ 0.05), and its bunions-metatarsophalangeal joint function score was higher than that of the control group (P ＜ 0.05). The scores of pain, sleep quality and mental state in the two groups after nursing were lower than those before nursing (P ＜ 0.05), and the scores of life quality and comfort were higher than those before nursing (P ＜ 0.05). After nursing, the scores of comfort and quality of life in the observation group were higher than those in the control group (P ＜ 0.05), and the scores of pain, sleep quality and mental state were lower than those in the control group (P ＜ 0.05). The total satisfaction rate of the observation group was higher than that of the control group (P ＜ 0.05). Conclusion During the minimally invasive mini-incision correction of hallux valgus, comprehensive nursing can effectively relieve the pain, improve the comfort, improve the sleep quality, and improve the psychological state of the patients. It is beneficial to the recovery of the function of the hallux-metatarsophalangeal joint function and the improvement of life quality and the evaluation of nursing service.
		2019 Vol. 16 (8): 165-168 [Abstract] ( 174 ) HTML (1 KB) PDF (603 KB) ( 88 )

	169	Influence of evidence-based nursing intervention for the patients with liver cirrhosis combined with upper gastrointestinal hemorrhage
		LANG Ying ZHAO Lei▲ HAN Wei XUN Jingwen XU Wenjing
		Objective To investigate the influence of evidence-based nursing intervention for the patients with liver cirrhosis combined with upper gastrointestinal hemorrhage. Methods From January 2014 to June 2016, 88 patients with liver cirrhosis combined with upper gastrointestinal bleeding admitted to the Department of Gastroenterology of Beijing Luhe Hospital were enrolled as the study subjects. The patients were numbered according to the order of admission, then equally divided into control group and experimental group by random number table, there were 44 cases in each group. The control group received routine nursing intervention, the experimental group received evidence-based nursing intervention beside of routine nursing intervention. The changes of scores of SAS and SDS in the two groups before and after intervention were observed. The difference of incidence of reheamorrhagia within half a year, success rate of hemostasis, hospital stay and degree of nursing satisfaction between the two groups were compared. Results The scores of SAS, SDS in both groups were significantly reduced after intervention（P ＜ 0.05）, and the scores of SAS, SDS in the experimental group after intervention were significantly lower than those in control group, and the diffenences were statistically significant （P ＜ 0.05）. Compared with the control group, the success rate of hemostasis in the experimental group was higher, the incidence of rehaemorrhagia within half a year was much lower, hospital stay was shorter, all of the differences were statistically significant（P ＜ 0.05）. The degree of nursing satisfaction in the intervention group was significantly higher than that of control group（P ＜ 0.05）. Conclusion Application of evidence-based nursing intervention for the patients with liver cirrhosis combined with upper gastrointestinal hemorrhage can improve the states of anxiety, depression, promote hemostasis, decrease the incidence of rehaemorrhagia, shorten hospital stay, increase the degree of nursing satisfaction, which is worthy of clinical promotion and application.
		2019 Vol. 16 (8): 169-172 [Abstract] ( 207 ) HTML (1 KB) PDF (597 KB) ( 54 )

	173	Effects of nasal feeding tube combined with careful nursing on enteral nutrition and pulmonary infection in patients with cerebralstroke
		XU Hong WANG Yingli XIN Yuying
		Objective To explore the effects of nasal feeding tube combined with careful nursing on enteral nutrition and pulmonary infection in cerebralstroke patients. Methods From April 2016 to March 2018, 116 patients with cerebralstroke admitted into the Department of Nursing in Dalian Central Hospital were divided into control group and observation group according to random number table method, with 58 cases in each group. The control group was given nasal feeding tube combined with routine nursing treatment, and the observation group was given nasal feeding tube combined with careful nursing treatment. The nutrition index, immunity level, the score of national institutes of health stroke scale score (NIHSS) and the rate of pulmonary infection were compared between the two groups. Results After nursing, the levels of nutritional indicators in the two groups all increased, and those in the observation group were higher than those in the control group, the differences were statistically significant (P ＜ 0.05). The levels of IgG, IgA and IgM all descended, and the levels of IgG, IgA and IgM in the observation group were higher than those in the control group, the differences were statistically significant (P ＜ 0.05). The NIHSS scores of patients in both groups were lower than those before nursing, and the NIHSS scores of patients in the observation group were lower than those in the control group, the differences were statistically significant (P ＜ 0.05). The rate of pulmonary infection in the observation group was 10.31%, which was significantly lower than that 25.90% in the control group, the difference was statistically significant (P ＜ 0.05). Conclusion Nasal feeding tube combined with fine nursing can ensure the nutrition supply in stroke patients, resist the stress effect of the body, reduce the occurrence of pulmonary infection, and promote the recovery of patients′ immune function and neurological function.
		2019 Vol. 16 (8): 173-176 [Abstract] ( 198 ) HTML (1 KB) PDF (596 KB) ( 101 )

	177	A case of professor Zhu Zhangzhi′s experience in treating neurogenic bladder of diabetes by using the method of acrid openning and bitter descending
		LIU Wenhua1 DENG Bin2 ZHU Zhangzhi3
		The diabetic neurogenic bladder is one of the chronic complications of diabetes mellitus. Its clinical manifestations mainly include bladder emptying disorder, urinary retention and urinary incontinence, etc., which has a great impact on the quality of life of patients. This paper introduces a case of diabetic neurogenic bladder diagnosed. The main symptoms of the patient are dysuria, dribbling urine, no urgency of urination and urodynia, nocturia for about half an hour, accompanied by abdominal distension, heart irritation, stomach noise, heartburn and other symptoms. The symptoms of the patients were not significantly improved after visiting to several hospitals. Professor Zhu Zhangzhi used the method of acrid openning and bitter descending to regulate the qi-lifting mechanism of spleen and stomach, then warm yang to invigorate the kidney and treat cold and heat. After treatment, the above symptoms basically disappeared. Therefore, the treatment of neurogenic bladder of diabetes should be based on the root of Taiyin, and help Shaoyin and Yuanyang. It can be seen that the concurrent treatment of both cold and heat is effective by the method of acrid openning and bitter descending.
		2019 Vol. 16 (8): 177-179 [Abstract] ( 185 ) HTML (1 KB) PDF (331 KB) ( 207 )

	180	Comparative analysis of 4 types of hospitals in China based on bibliometrics analysis
		SONG Haiyan MENG Kai
		Objective To reveal the current researches of four types of hospitals such as research hospitals and college hospitals in recent years in China, compare their differences, and provide reference for classification and development of large public hospitals under the background of graded diagnosis and treatment. Methods Using the research papers of this four types of hospitals recorded in CNKI and Wanfang database through NoteExpress software since 2000 to 2016 as data basis, some bibliometric indexes were analyzed, including the title, publication year, first author, abstract, keywords and other content, EXCEL database was built and statistical analysis was performed. Results It was found that the connotations of four different types of hospitals were similar, with emphasis on scientific research innovation and the transformation of results. The research content was also concentrated in clinical, teaching, personnel training, scientific research and cultural construction, but the construction background was different, the development level was not balanced and the construction path was still being explored. Conclusion The 4 types of hospitals were proposed to conform to the development of the times, but there were problems of small differences in connotation and unclear construction paths. Whether the 4 types of hospitals should be raised should be discussed. The classification of science in large public hospitals also needs to be further explored.
		2019 Vol. 16 (8): 180-封三 [Abstract] ( 188 ) HTML (1 KB) PDF (643 KB) ( 136 )

	185	Analysis on coping strategies of network public opinion in new media era
		LI Jiayao ZHANG Nan
		With the rapid development of new media technology, the field of health communication has entered a state of barbarism. On the one hand, the development and research of health communication research is not systematic in China. On the other hand, it is a mismatch between public health literacy and health needs, which leads to the fact that health communication is often facing the dilemma of rumor rather than truth. To study the coping strategies of health communication network public opinion in the new media era, it is well-founded to clarify the concept of health communication, then analyze the characteristics of health communication in the new media era, and finally formulate corresponding strategies for Internet public opinion. The spread effect of health communication is reflected in public opinion. The characteristics of the new media age influence the public opinion. Based on the new media communication that exists in the establishment of "relationship", the main stream of communication is user generated content (UGC). The mode of communication is complex and changeable, which greatly expands the extension of communication. Therefore, in the face of Internet public opinion, strategy formulation should start from the characteristics of communication, attach importance to collection of public opinion, pay equal attention to prevention and control, improve the ability of new media′s public opinion guidance, and establish and improve relevant legal system.
		2019 Vol. 16 (8): 185-封三 [Abstract] ( 224 ) HTML (1 KB) PDF (793 KB) ( 155 )

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