青蒿虎酯对大鼠睾丸扭转复位后缺血再灌注损伤的影响

Abstract
Figure/Table
References (20)
Related Citation (15)

Download: PDF (1002 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the effects of artesunate on ischemia-reperfusion injury after testicular torsion/detorsion in rats. Methods Eighteen adult healthy male Sprague Dawley rats were divided into control group, ischemia group and artesunate group by random number table method, with 6 rats in each group. The model rats with testicular torsion/detorsion were made (720° for 2 h). The artesunate group was given artesunate 25 mg/(kg·d) by gavage administration at 3 d before operation. The control group and ischemia group were given same volume of distilled water by gavage administration. After detorsion for 4 h, the rats were sacrificed and the testicular tissue was obtained, the contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were detected, and the histopathological parameters and apoptosis index (AI) were tested by HE staining and TUNEL staining. The expression of poly (ADP-ribose) polymerase (PARP)-1 and apoptosis-inducing factor (AIF) in testicular tissue of each group was detected by Western blot. Results Compared with control group, the SOD activity and the diameter of seminiferous tubules in ischemia group and artesunate group were significantly decreased, while the MDA content, apoptosis index, the expression of PARP-1 and AIF in ischemia group and artesunate group were significantly increased, the differences were statistically significant (P ＜ 0.05). Compared with ischemia group, the SOD activity and the diameter of seminiferous tubules in artesunate group were significantly increased, while the MDA content, apoptosis index, the expression of PARP-1 and AIF in artesunate group were significantly decreased, the differences were statistically significant (P ＜ 0.05). Conclusion Artesunate has protective effects on ischemia-reperfusion injury after testicular torsion/detorsion, its mechanism may be achieved through inhibiting apoptosis and oxidative stress.

Key words： Artesunate Testis Torsion Ischemia

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Lei WENG Xiaodong CHEN Zhiyuan LIU Xiuheng

Cite this article:

WANG Lei WENG Xiaodong CHEN Zhiyuan LIU Xiuheng. Effects of artesunate on ischemia-reperfusion injury after testicular torsion/detorsion in rats[J]. 中国医药导报, 2019, 16(8): 18-21.

URL:

http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I8/18

[1] Yazawa H，Sasagawa I，Suzuki Y，et al. Glucocorticoid hormone can suppress apoptosis of rat testicular germ cells induced by testicular ischemia [J]. Fertil Steril，2001，75（5）：980-985.
[2] 赵豫刚，郑新民，周吉，等.睾丸扭转复位后生精上皮细胞核转录因子表达的改变及其凋亡[J].中华医学杂志，2006，86（20）：1381-1385.
[3] Kapoor S. Testicular torsion：a race against time [J]. Int J Clin Pract，2008，62（5）：821-827.
[4] Lysiak JJ，Nguyen QA，Turner TT. Peptide and nonpeptide reactive oxygen scavengers provide partial rescue of the testis after torsion [J]. J Androl，2002，23（3）：400-409.
[5] Filho DW，Torres MA，Bordin AL，et al. Spermatic cord torsion，reactive oxygen and nitrogen species and ischemia-reperfusion injury [J]. Mol Aspects Med，2004，25（1/2）：199-210.
[6] Efferth T，Dunstan H，Sauerbrey A，et al. The anti-malarial artesunate is also active against cancer [J]. Int J Oncol，2001，18（4）：767-773.
[7] 许光兰，梁劲松，以敏，等.青蒿琥酯对肺纤维化大鼠肺组织转化生长因子β1的影响[J].广西医科大学学报，2008， 25（3）：400-403.
[8] 刘文东，李英娴，娄婷婷，等.青蒿琥酯对肝纤维化大鼠肝组织基质金属蛋白酶表达的影响[J].天津医科大学学报，2013，19（5）：362-364，372.
[9] 孙倩，曾诚，段薇娜，等.血管紧张素-（1-7）/血管紧张素-Ⅱ通过调节Nrf2/HO-1通路在糖尿病肾脏缺血再灌注损伤中的作用及机制[J].中国医药导报，2017，14（20）：12-15，35.
[10] 胡东来，舒强.新生儿睾丸扭转的临床分析[J].中华泌尿外科杂志，2017，38（2）：118-121.
[11] Lysiak JJ，Turner SD，Nguyen QA，et al. Essential role of neutrophils in germ cell-specific apoptosis following ischemia/reperfusion injury of the mouse testis [J]. Biol Reprod，2001，65（3）：718-725.
[12] 张华锋，赵佳，徐道明.白藜芦醇对大鼠睾丸扭转损伤的保护作用[J].中华实验外科杂志，2015，32（6）：1473.
[13] 郭晶晶，俞丽云，黄美，等.持续低流量复灌注对兔睾丸不全扭转细胞凋亡的影响[J].中国男科学杂志，2015， 29（1）：22-27.
[14] 刘子明，郑新民，李世文，等.大鼠睾丸扭转复位后的组织结构变化和相关因素的研究[J].医学新知杂志，2003， 13（1）：17-19.
[15] 张晓文，李建华，徐赫，等.丹参酮ⅡA对成年大鼠睾丸扭转的保护作用和机制研究[J].东南大学学报：医学版，2017，36（5）：795-799.
[16] Payabvash S，Kiumehr S，Tavangar SM，et al. Ethyl pyruvate reduces germ cell-specific apoptosis and oxidative stress in rat model of testicular torsion/detorsion [J]. J Pediatr Surg，2008，43（4）：705-712.
[17] 徐波，陈海琛，黄国显，等.褪黑激素对大鼠睾丸扭转缺血和再灌注损伤的保护作用[J].中华小儿外科杂志，2012， 33（7）：532-535.
[18] 官毅，郑新民，杨志伟，等.左旋卡尼汀对大鼠睾丸缺血再灌注损伤的防护作用[J].中华医学杂志，2009，89（26）：1858-1861.
[19] 刘桂勇，刘修恒，王磊，等.青蒿琥酯对大鼠肾脏缺血再灌注后纤维化改变的保护作用[J].中华实验外科杂志，2015，32（10）：2485-2487.
[20] Germain M，Affar EB，D'Amours D，et al. Cleavage of automodified poly（ADP-ribose）polymerase during apoptosis. Evidence for involvement of caspase-7 [J]. J Biol Chem，1999，274（40）：28379-28384.