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| Effects of different acupuncture methods in Mongolian Medicine on monoamine neurotransmitters and PKA/CREB/BDNF pathway in prefrontal lobe of depressed rats |
| AI Liya1 YANG Lijuan2 LIU Juntong2 AO Qier3 Saiyinchaoketu3 |
1.Graduate School, Inner Mongolia Medical University, Inner Mongolia Autonomous Region, Hohhot 010110, China;
2.School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China;
3.the Fifth Department of Rehabilitation, Inner Mongolia International Mongolian Hospital, Inner Mongolia Autonomous Region, Hohhot 010065, China |
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Abstract Objective To observe the effects of different acupuncture methods on the neurotransmitter and protein kinase (PKA) activity, cyclophosphamide receptor binding protein (CREB) phosphorylation and brain-derived neurotrophic factor (BDNF) expression in the prefrontal cortex of depressed rats. To explore the antidepressant mechanism of different acupuncture methods in Mongolian medicine. Methods A total of 40 male Sprague-Dawley rats were divided into blank group, model group, Mongolian medical silver needle group, Mongolian medicine three balanced needle group and drug group according to random number table method, with 8 rats in each group. Rats in the other groups were orphaned except for the blank group and were given unpredictable 7 different stress stimulation for 28 days. Among them, 4 rats in the blank group were raised in each cage and fed with normal drinking water without any stimulation; the model group did not receive any treatment; the Mongolian medical silver needle group received the silver needle for stimulation of the Bada dry point, Heart acupuncture points and black and white points 1 h before the daily stress stimulation; acupuncture and acupuncture points of the Mongolian medicine three balanced needle group were the same as those in the Mongolian medical silver needle group, but one acupoint was selected daily for warm needle stimulation; the drug group was given 1 hour before stress stimulation daily, Fluoxetine hydrochloride was formulated into a 1 mg/1 mL solution in a 0.9% sodium chloride solution, and the drug group received intragastric administration according to a body weight of 2 mg/kg. After 28 days, the rats were decapitated and the prefrontal lobe was taken. The 5-hydroxytryptamine (5-HT), norepinephrine (NE), dopamine (DA) content, PKA activity, CREB phosphorylation and BDNF expression level were detected by enzyme-linked immunosorbent assay and Western blot. Results Compared with the blank group, the contents of 5-HT, NE and DA in the prefrontal cortex of the model group were significantly decreased after 28 days of modeling (P ＜ 0.01), and the levels of PKA activity, CREB and BDNF expression were also significantly decreased (P ＜ 0.05). Compared with the model group, the contents of NE, DA and 5-HT in the prefrontal cortex of Mongolian medicine three balanced needle group and drug group were significantly increased, the differences were statistically significant (P ＜ 0.05), the contents of DA and 5-HT in the prefrontal cortex of Mongolian medical silver needle group were significantly increased, the differences were statistically significant (P ＜ 0.05). Compared with the model group, the levels of PKA activity, CREB and BDNF expression in the prefrontal cortex of Mongolian medical silver needle group, Mongolian medicine three balanced needle group and drug group were significantly increased, the differences were statistically significant (P ＜ 0.05). Conclusion Different needles of Mongolian medicine can effectively increase the content of 5-HT/NE/DA, the activity of PKA and the expression of CREB-BDNF, suggesting that different needles of Mongolian medicine have antidepressant effects.
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[1] Goda R，Otsuka T，Iwamoto A，et al. Serotonin levels in the dorsal raphe nuclei of both chipmunks and mice are enhanced by long photoperiod，but brain dopamine level response to photoperiod is species-specific [J]. Neurosci Lett，2015，593：95-100.
[2] Huang SJ，Zhang XH，Wang YY，et al. Effects of Kaixin Jieyu Decoction on behavior，monoamine neurotransmitter levels，and serotonin receptor subtype expression in the brain of a rat depression model [J]. Chin J Integr Med，2014，20（4）：280-285.
[3] Park H，Poo MM. Neurotrophin regulation of neural circuit development and function [J]. Nat Rev Neurosci，2013，14（1）：7-23.
[4] 李昆，孙国平，卢豪忠，等.曲古抑菌素A对抑郁模型大鼠行为及脑源性神经营养因子表达的作用研究[J].中国医药导报，2017，14（24）：25-29.
[5] Shinizu E，Hashimoto K，Okamura N，et al. Alterations of serum levels of brain-derived neurotrophic factor（BDNF）in depressed patients with or without antidepressants [J]. Biol Psychiatry，2003，54（1）：70-75.
[6] 邓海峰，孙缦利，吴琼，等.毛蕊花糖苷通过调控BDNF-TrkB信号通路改善CUMS大鼠的抑郁样行为[J].中国病理生理杂志，2018，34（9）：1633-1637.
[7] Katz RJ，Baldrighi G. A further parametric study of imi-pramine in an animal model of depression [J]. Phamacol Bichem Behav，1982，16（6）：969-972.
[8] 许晶，李晓秋.慢性应激抑郁模型的建立及其评价[J].中国行为医学科学，2003，12（1）：14-17.
[9] 赛音朝克图，白淑英，景泉凯，等.蒙医三根平衡针对慢性应激抑郁模型大鼠海马神经元形态及BDNF-CREB信号通路的影响[J].中华中医药杂志，2017，32（8）：3667-3670.
[10] Ruda-Kucerova J，Amchova P，Havlickova T，et al. Re- ward related neurotransmitter changes in a model of depression：an in vivo microdialysis study [J]. World J Biol Psychiatry，2015，16（7）：521-535.
[11] Imbe H，Kimura A. Repeated forced swim stress affects the expression of pCREB and △FosB and the acetylation of histone H3 in the rostral ventromedial medulla and locus coeruleus [J]. Brain Res Bull，2016，127：11-22.
[12] Kudo T， Uda S，Tsuchiya T，et al. Laguerre Filter Analysis with Partial Least Square Regression Reveals a Priming Effect of ERK and CREB on c-FOS Induction [J]. PLoS One，2016，11（8）：e0160548.
[13] Thome J，Sakai N，Shin K，et al. cAMP response element-mediated gene transcription is upregulated by chronic antidepressant treatment [J]. J Neurosci，2000，20（11）：4030-4036.
[14] Sharma N，Lopez DI，Nyborg JK. DNA binding and phosphorylation induce conformational alterations in the kinase-inducible domain of CREB. Implications for the mechanism of transcription function [J]. J Biol Chem，2007， 282（27）：19872-19883.
[15] Marzieh Rezai，Mehdi Mahmoodi，Ayat Kaeidi，et al. Effect of crocin carotenoid on BDNF and CREB gene expression in brain ventral tegmental area of morphine treated rats [J]. Asian Pacific Journal of Tropical Biome-dicine，2018，8（8）：387-393.
[16] Mlyniec K，Budziszewska B，Holst BA，et al. GPR39（Zinc receptor）knockout mice exhibit Depression-Like behavior and CREB/BDNF Down-Regulation in the hippocampus [J]. Int J Neuropsychopharmacol，2015，18（3）：1-8.
[17] Razavi BM，Sadeghi M，Abnous K，et al. Study of the Role of CREB，BDNF，and VGF Neuropeptide in Long Term Antidepressant Activity of Crocin in the Rat Cerebellum [J]. Iran J Pharm Res，2017，16（4）：1452-1462.
[18] Kim Y，Lee HY，Cho SH. Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor（BDNF）Expression in Chronic Stress Model of Mice [J]. J Acupunct Meridian Stud，2017，10（6）：402-408.
[19] Taliaz D，Stall N，Dar DE，et al. Knockdown of brain-derived neurotrophic factor in specific brain sites precipitates behaviors associated with depression and reduces neurogenesis [J]. Mol Psychiatry，2009，15（1）：80-92.
[20] Aznar S，Klein AB，Santini MA，et al. Aging and Depression Vulnerability Interaction Results in Decreased Serotonin Innervation Associated With Reduced BDNF Levels in Hippocampus of Rats Bred for Learned Helplessness [J]. Synapse，2010，64（7）：561-565.
[21] Mattson MP，Maudsley S，Martin B. BDNF and 5-HT：a dynamic duo in age-related neuronal plasticity and neurodegenerative disorders [J]. Trends Neurosci，2004，27（10）：589-594. |
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